Rh2DG693700

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003475
Physical Location & Seq
Reverse (-)
91009581 .. 91017401
7821 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG693700.1

Sequence Viewer

Length: 4482 bp
ATGATGGCTCACGGAAAAGTCAGTCTCCCTCAAGATCTCCTCCCCTCGCCGATCTTCTCCGCCGCCAAAGATGGAGCCTTGGAAGGAAATGCTGAGGGAAAGGCTCTAACGGGGTCACTTGATGAGTCAAAAGATCATTTAGCATCTGAGAGCAGCATACCTTTGTCACCACAATGGCTACATGCTAAATCAGTTGAGGCTAAGCCACTTGCAAGTGGAACATCAGGAGAAATCCATGCACCAGGGTCTTTGTCGCATGGTAACTCTATTGATTCCAATGCGAGAGACATTTGGCGTTCAGATGGTTCTCGGGACAGAAAAGATTGGAGGAGGAATGCACCTGATTTAGATGTTAACCGTCGCTGGCGTGAGGAGGAGAGAGAAACAGGCTTACTTGGTAGGAGGGATCGCAAAAAAGAAGATCGCCGTGTAGGAGTCACTTCAACTAGGGATGTTGCTGAGAATCGAGCAGAAGATCATCGAGCAGGTGGCCCTTCTACTAGGGATGTTAGTGAGAATAAAATTTTGTCTTCTGATCGCTGGCATGAATCCCGGAGAGACAACAAATGGTCATCAAGGTGGGGCCCTGAAGATAAAGACAAGGACTCTCGAATAGAGAAGACAGAACTTGAGAGAGAAGATGCTCATGTTGACAAGCAGTCTCTTGTCAGTAGCAATCGTGCAATTCCTGAACGTGATGGTGATTCTCGCGATAAATGGAGGCCACGCCATCGCATGGAGGTTCAGTCCGGGGGGCCAGCCCCATACCGTGCCGCACCGGGATTTGGGATGGCCAGAGGACAGGTGGAAAAAGTAGGATTTGCTTCTGGCCGAGGGAGGTCCAAGACTAATGGTAGCCTACAGATTGGAAAACCTTCCTCTGTATCCACTATCGGGTCTGTTCTGGATCAGAATCAAAGTGTATTGGGAAACGCTGGCCCCTTTAATGAGAAGTATCGTTACCCCAGGGGAAAGCTCCTTGATATCTATCGTAAGCAAAAGATAGAATCAACTTTCGATATTGTGCCTGATGGGATGGAGCATGTCTCTCCAATTACCCAAGTGGGCTCTATTGAGCCATTGGCCTTTTCTGCACCTCATGCAGAAGAAGAGGCTGTTATGGAAGATATATGGAAGGGAAGAATCACTAGCAGTGAAATGTTGTGCAGTTCCTCCAGAGAAAAGAATGTGTTAAATGATGATGGAACTAGTAATGCCTCCTTAAGAAAGGAAGATCAAAAGATTTCACTGCAGACTGAAGAAAATGCCCTATCTACTGGAGAGCCCATCTTAAACAATTCTTTTCCAGTTATCGGTACTGAGGTCTCAACTATCTGTGGCCCACGGACACATATTTTAAATGAATGGGTTGAAGGTGAGCGCAGAGTTCCTATTGCCACAGCAGTTGCTGATGGATCAAATCTGGTTGCTTCAAAGAACTATGATTGCAAAAGTGCTGGGGAGATTGATGGCCTCAGTAATAGTGTCGCTGAACTAAGAATTTCTGGATGCCAGGATGTGAGAGATTTTGGTCTTCCAAAGCATTCTATGTCGGAAAATTTTGAACCAGCTACTACTGTGATTGGTAGCCAGCTTCCTGATGATTCAAGTTCACTTTTTCACATTCCTTCTCTACAGCAAACCTCGAGTAGTGATCAATATGTCAAGAGCAACGAGAAGGCAGATCTGGAAAGGGTTGTCTCATTTGAGGATTTAAGTTTATGCTATCTTGATCCTCAAGGAAACATCCAGGGACCTTTCCTGGGGATCGACATAATTTCATGGTTTGATCAAGGATTCTTTGGAACTGACTTACTGGTGCGCTTGGCTGATGCCCCTAATGGTTCTCCTTTTCAAGAACTCGGTGAAATGATGCCAAATCTGAACACTAAATCTGGATTGGTCTCCAACTCCAATTTGCCTACCAACGTGGAATCATTTGGTGGTGTTGGAAGCATCCTGGAAGAGAGAAAAACTGCTCCTCACTATGAGGAGTCTAACATTCTAAATATCCAACACTGGACTTCATCTGGACTTGAACCTACCTTGAGTGATGATTTGCACTCGAGAATACCTAATAATGGTTACAACTCTGAACTCCAGTACCTTGATAATCAAAGGTTTCAAAATTACGTTGAAGAAGATGAGGAAATAGTGTTTCCCGGAAGGCCTAAAAGTAGCAGTGATTTTCCTCTGAGGAGACCTTCAGCTGATATTCAGGGCTCATTTTCAAATTCCCCAAGCCTTCCTTCCCTTTCAAATGAAGTTTCCGAAAGTGACCTGCCTAATCGCCAGGATGATAAGTTGCACCCTTTTGGCCTGTTGATGTCTGAGCTCAGAGGCAGCTCTCATTTAAGACCTGCTCAAGCTTCCAATGTGTCTTTAGGCATGGATGATCAGGCTCAGTTTAGAGATACCTTGTTTGAGGGAGGTATCTCCAAACTGAGTTGGTTGAATAATCCTAATATCCCAATGCATCAAGTAGTGCCTGATATGGAACATCTAATAGAACTACAGCGTCAAGAGCAGCAGCATCGCCTATTGGAGCTTCAGCAACAACGCCAGTTTGAGTTTCATCGTCAGCAGCAGCACGAGTTGGAATTTCAACAGCAGCAGCAGCAGCAGCGGCAATTGGAACTCCAGCAACAGCGCCAGTTGGAGCTACAGCAGCAGCGCCAGTTGGAACTTCACCACTTGGAGCTTCAGCAGCAGCACCAGTTGGAGCTACAGCTGCAGCGCCAGTTGGAGCTACAGCAACAGCGCCAGTTTGAGCTACAGCAACAGCGCCAGTTGGAGCTACAGCAACAACGCCAGTTGGAGCTTCAGCAACAGCAGCAACACCATTTGGAGCTTCAACAACAGCAGCAGCGGAAGCTGGAGCTTCAGCAGCTGCAGCGTCATCAGCAACAGCGGCAGTTGGAGTTTCAGCAGCAGCGCCAATTGGAGCTTCAGCAACAGCAGCATCAATTGGAGCTTCCGCCTCAGCACCACTTGCAGCAGCACCTTCGCCATCAACAAAAATTGCAGCAACAACAGCAGTCCCAAGCCGAACAATTGCTCCTCGAACAATTATTGAATCGACAAATGTCTGATGGTTATGGGCAGTGGAAGATGGATGCTACACGAGCCAACCTTCATGATCAGTTACATTCCAGTAACCACCTTCCAAATGATTTGCACCATGTATCTCATTCATCAAGGCATGATCCCTCGCTAGAGCAGATCATCCAAGCAACTATGGGCGAGGGTGCACTCCAAGGACAGACTGATTTTTTTGACCTCATATCGCAGGCCAAGCAAGGGAATAGGAACACTTCAGAGCTGCCACTTCGTCTTAATGAACAAGAGTTGCAAGCACAGCAATTATCTTTGGCATTTAGAGAGCAGCAGCAGATAGAAGGGGAAAGGCGTATTGGTGGGCCTTGGTTTGCAGATGAAGCAGGTCCTTTTTTGAGGGATCCCGTTGGGCATCACCAGGCACACATGTTGGGGCATAATTCTTCAGAAAATTACCAGCAACAGCAGAGGTTTCCATCCAATGAACAGGAACCAAGCCATCACGATTGGAGTCATGCCTCTAAGGAGAGACCTCAGGGAGGGTTTTATGAATCTAACTCTATGGAATTTGATAGATTCTCTGTTCCTGCTGGTTCTCATGGAATGAACTTGGATATTGCAAACATACGTGGACAAGATCTAGATGTGAGGGAACAATATCGGCTTAATTCCTCTATTGATGAGCGTGGATCTCTATCATCTGGCGTAACCTCTCACCGTCGTTTGGTTTCTGATGAGTTTTATGCTTCTCATCCAGTTACTTTTGAAAGCTTGCCATCTGGGAACAATGGGCAGCTAGAAAGTAGCTGCACTGAAGCAGTAATGCCTTATTTGCATCTTGATGCTGAGCAAAAACGACGAGACACGGAAGACACCATTGCTTTTGCTGCTTCAAATAGATTGGCTTCAACTAACGGTGATAAGGAACACTCCAAACAAATTTTGATGGACCTTCATCAAAAACTTGGTCATCAGCCTACACAGTCATCAGAAGTTGATTATCAGCATCACCTATCCTCATCCAGAAGTCAGGGAGGCTCTGTACATCTTCCTTTAAATCTTCTTCCAGATCAGTCAGTTGGTATGAGCAACTGCTTCACAGAAGGCCCACAGAGTTCAAATTTTAGTCTCTTGTTGCAAGATCAGTTGGGTAGTTATGGTATGAATGAGCAACCAAGTAATCTGGCAAGAAGATCTGATTCCAGAGCATTTATGGACGCTCAATTCTTGTCAGGAACCAGGGATGCTCCTCATGAAGGCAACATGGCAAACCAACAAGACACAGCCATTGAGAGCGGGGAACTACCTAGTAATACCCATAGCAGGCATAGCTCACTGAGCAGTGCTGGTGGATGTGGGGGTTTTCACAGCTCTGAGATGGGATTAGATAAATCACTTGGAGAAGATGTTTCTGATGGGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1493

Amino Acids

168.37

Weight (kDa)

5.43

Isoelectric Point (pI)

63.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 576 - 620 1.3e-10 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 476
Acc36I ACCTGC 4 cut(s) 476, 2289, 2368, 3431
AccB7I CCANNNNNTGG 1 cut(s) 736
AccBSI CCGCTC 1 cut(s) 4353
AccII CGCG 1 cut(s) 711
AciI CCGC 8 cut(s) 60, 63, 774, 2626, 2869, 2911, 2978, 4353
AclWI GGATC 9 cut(s) 414, 915, 1423, 1727, 1775, 3200, 3452, 3465, 3754
AcoI YGGCCR 2 cut(s) 792, 829
AcsI RAATTY 8 cut(s) 522, 1500, 1558, 2233, 2600, 3623, 3996, 4177
AfaI GTAC 3 cut(s) 1318, 2105, 4101
AflII CTTAAG 1 cut(s) 1222
AflIII ACRYGT 1 cut(s) 3483
AhdI GACNNNNNGTC 1 cut(s) 658
AhlI ACTAGT 1 cut(s) 1208
AjnI CCWGG 9 cut(s) 241, 965, 1512, 1749, 1761, 1959, 2292, 3474, 4295
AjuI GAANNNNNNNTTGG 6 cut(s) 2233, 2265, 2931, 2963, 3396, 3428
AloI GAACNNNNNNTCC 2 cut(s) 3042, 3074
Alw21I GWGCWC 2 cut(s) 2337, 3253
Alw44I GTGCAC 1 cut(s) 3249
AlwI GGATC 9 cut(s) 414, 915, 1423, 1727, 1775, 3200, 3452, 3465, 3754
AlwNI CAGNNNCTG 2 cut(s) 1409, 2890
Ama87I CYCGRG 3 cut(s) 309, 1645, 2065
ApaI GGGCCC 1 cut(s) 587
ApaLI GTGCAC 1 cut(s) 3249
ApoI RAATTY 8 cut(s) 522, 1500, 1558, 2233, 2600, 3623, 3996, 4177
ArsI GACNNNNNNTTYG 2 cut(s) 4009, 4041
Asp700I GAANNNNTTC 1 cut(s) 874
AspLEI GCGC 8 cut(s) 1383, 1824, 2652, 2676, 2739, 2763, 2787, 2937
AsuC2I CCSGG 4 cut(s) 553, 751, 780, 2163
AsuHPI GGTGA 9 cut(s) 159, 713, 1388, 1877, 2681, 3464, 3764, 3986, 4058
AvaI CYCGRG 3 cut(s) 309, 1645, 2065
AvaII GGWCC 4 cut(s) 840, 1754, 3443, 4006
AxyI CCTNAGG 1 cut(s) 3591
BaeGI GKGCMC 2 cut(s) 587, 3253
BaeI ACNNNNGTAYC 4 cut(s) 1308, 1341, 2087, 2120
BalI TGGCCA 1 cut(s) 794
BamHI GGATCC 1 cut(s) 3457
BanII GRGCYC 5 cut(s) 587, 1070, 1287, 2225, 2337
BarI GAAGNNNNNNTAC 2 cut(s) 944, 976
BauI CACGAG 2 cut(s) 2591, 3123
BbsI GAAGAC 4 cut(s) 522, 626, 1526, 3933
Bbv12I GWGCWC 2 cut(s) 2337, 3253
BbvCI CCTCAGC 2 cut(s) 93, 2982
BceAI ACGGC 1 cut(s) 411
BcgI CGANNNNNNTGC 4 cut(s) 2516, 2550, 2987, 3021
BciT130I CCWGG 9 cut(s) 243, 967, 1514, 1751, 1763, 1961, 2294, 3476, 4297
BciVI GTATCC 1 cut(s) 895
BclI TGATCA 4 cut(s) 1654, 1789, 2395, 3139
BcnI CCSGG 4 cut(s) 553, 751, 780, 2163
BcuI ACTAGT 1 cut(s) 1208
BfaI CTAG 8 cut(s) 447, 501, 1149, 1209, 3215, 3698, 3854, 4365
BfoI RGCGCY 6 cut(s) 2653, 2677, 2740, 2764, 2788, 2938
BfrI CTTAAG 1 cut(s) 1222
BfuAI ACCTGC 4 cut(s) 476, 2289, 2368, 3431
BfuI GTATCC 1 cut(s) 895
BglII AGATCT 4 cut(s) 34, 1684, 3694, 4250
BlpI GCTNAGC 2 cut(s) 201, 3903
Bme18I GGWCC 4 cut(s) 840, 1754, 3443, 4006
BmeRI GACNNNNNGTC 1 cut(s) 658
BmeT110I CYCGRG 3 cut(s) 309, 1645, 2065
BmiI GGNNCC 9 cut(s) 76, 584, 585, 756, 940, 1755, 3459, 3549, 4294
BoxI GACNNNNGTC 1 cut(s) 3085
BpiI GAAGAC 4 cut(s) 522, 626, 1526, 3933
BplI GAGNNNNNCTC 2 cut(s) 1031, 1063
BpmI CTGGAG 5 cut(s) 1159, 1299, 2084, 2624, 2897
Bpu10I CCTNAGC 2 cut(s) 93, 2982
Bpu1102I GCTNAGC 2 cut(s) 201, 3903
BpuEI CTTGAG 5 cut(s) 15, 650, 1722, 2068, 2349
BpuMI CCSGG 4 cut(s) 553, 751, 780, 2163
BsaAI YACGTR 1 cut(s) 3686
BsaBI GATNNNNATC 3 cut(s) 912, 987, 3751
BsaI GGTCTC 4 cut(s) 1330, 1909, 2194, 3580
BsaXI ACNNNNNCTCC 8 cut(s) 731, 761, 3042, 3072, 4083, 4113, 4449, 4479
Bse21I CCTNAGG 1 cut(s) 3591
Bse3DI GCAATG 1 cut(s) 3933
Bse8I GATNNNNATC 3 cut(s) 912, 987, 3751
BseBI CCWGG 9 cut(s) 243, 967, 1514, 1751, 1763, 1961, 2294, 3476, 4297
BseJI GATNNNNATC 3 cut(s) 912, 987, 3751
BseMI GCAATG 1 cut(s) 3933
BseRI GAGGAG 9 cut(s) 29, 343, 386, 389, 1971, 2006, 2212, 3050, 4296
BseSI GKGCMC 2 cut(s) 587, 3253
BseYI CCCAGC 1 cut(s) 1457
BsgI GTGCAG 3 cut(s) 1077, 1186, 3850
Bsh1236I CGCG 1 cut(s) 711
BsiHKAI GWGCWC 2 cut(s) 2337, 3253
BsiHKCI CYCGRG 3 cut(s) 309, 1645, 2065
BsiSI CCGG 4 cut(s) 553, 750, 779, 2163
BslFI GGGAC 3 cut(s) 326, 1767, 3025
BsmFI GGGAC 3 cut(s) 326, 1767, 3025
BsmI GAATGC 2 cut(s) 340, 1543
Bso31I GGTCTC 4 cut(s) 1330, 1909, 2194, 3580
BsoBI CYCGRG 3 cut(s) 309, 1645, 2065
Bsp120I GGGCCC 1 cut(s) 583
Bsp1286I GDGCHC 6 cut(s) 587, 1070, 1287, 2225, 2337, 3253
Bsp1407I TGTACA 1 cut(s) 4099
Bsp1720I GCTNAGC 2 cut(s) 201, 3903
Bsp68I TCGCGA 1 cut(s) 711
BspACI CCGC 8 cut(s) 60, 63, 774, 2626, 2869, 2911, 2978, 4353
BspFNI CGCG 1 cut(s) 711
BspHI TCATGA 2 cut(s) 3136, 4309
BspLI GGNNCC 9 cut(s) 76, 584, 585, 756, 940, 1755, 3459, 3549, 4294
BspMAI CTGCAG 3 cut(s) 1254, 2736, 2895
BspMI ACCTGC 4 cut(s) 476, 2289, 2368, 3431
BspPI GGATC 9 cut(s) 414, 915, 1423, 1727, 1775, 3200, 3452, 3465, 3754
BspTI CTTAAG 1 cut(s) 1222
BspTNI GGTCTC 4 cut(s) 1330, 1909, 2194, 3580
BsrBI CCGCTC 1 cut(s) 4353
BsrDI GCAATG 1 cut(s) 3933
BsrGI TGTACA 1 cut(s) 4099
BssSI CACGAG 2 cut(s) 2591, 3123
BssT1I CCWWGG 3 cut(s) 78, 3256, 3422
Bst2BI CACGAG 2 cut(s) 2591, 3123
Bst2UI CCWGG 9 cut(s) 243, 967, 1514, 1751, 1763, 1961, 2294, 3476, 4297
Bst4CI ACNGT 6 cut(s) 359, 770, 1579, 3776, 3974, 4041
Bst6I CTCTTC 2 cut(s) 1104, 1959
BstAFI CTTAAG 1 cut(s) 1222
BstAPI GCANNNNNTGC 2 cut(s) 1100, 2992
BstAUI TGTACA 1 cut(s) 4099
BstBAI YACGTR 1 cut(s) 3686
BstC8I GCNNGC 9 cut(s) 365, 542, 759, 937, 1592, 3291, 3354, 3830, 4382
BstDSI CCRYGG 1 cut(s) 1343
BstENI CCTNNNNNAGG 2 cut(s) 3594, 4311
BstFNI CGCG 1 cut(s) 711
BstH2I RGCGCY 6 cut(s) 2653, 2677, 2740, 2764, 2788, 2938
BstHHI GCGC 8 cut(s) 1383, 1824, 2652, 2676, 2739, 2763, 2787, 2937
BstNI CCWGG 9 cut(s) 243, 967, 1514, 1751, 1763, 1961, 2294, 3476, 4297
BstNSI RCATGY 3 cut(s) 185, 1046, 3487
BstPAI GACNNNNGTC 1 cut(s) 3085
BstSLI GKGCMC 2 cut(s) 587, 3253
BstUI CGCG 1 cut(s) 711
BstV2I GAAGAC 4 cut(s) 522, 626, 1526, 3933
BstX2I RGATCY 6 cut(s) 34, 1684, 3457, 3694, 3746, 4250
BstYI RGATCY 6 cut(s) 34, 1684, 3457, 3694, 3746, 4250
Bsu36I CCTNAGG 1 cut(s) 3591
BsuI GTATCC 1 cut(s) 895
BtgI CCRYGG 1 cut(s) 1343
BtgZI GCGATG 2 cut(s) 716, 2519
BtsI GCAGTG 5 cut(s) 1159, 1247, 2188, 3110, 4405
BtsIMutI CAGTG 8 cut(s) 1159, 1247, 2017, 2188, 3110, 3867, 4391, 4405
BtuMI TCGCGA 1 cut(s) 711
BveI ACCTGC 4 cut(s) 476, 2289, 2368, 3431
Cac8I GCNNGC 9 cut(s) 365, 542, 759, 937, 1592, 3291, 3354, 3830, 4382
CaiI CAGNNNCTG 2 cut(s) 1409, 2890
CciI TCATGA 2 cut(s) 3136, 4309
CfoI GCGC 8 cut(s) 1383, 1824, 2652, 2676, 2739, 2763, 2787, 2937
CseI GACGC 3 cut(s) 2507, 2885, 4283
Csp6I GTAC 3 cut(s) 1317, 2104, 4100
CspCI CAANNNNNGTGG 2 cut(s) 1387, 1422
CviQI GTAC 3 cut(s) 1317, 2104, 4100
DraI TTTAAA 2 cut(s) 1359, 4113
DriI GACNNNNNGTC 1 cut(s) 658
EaeI YGGCCR 2 cut(s) 792, 829
Eam1104I CTCTTC 2 cut(s) 1104, 1959
Eam1105I GACNNNNNGTC 1 cut(s) 658
EarI CTCTTC 2 cut(s) 1104, 1959
EciI GGCGGA 2 cut(s) 49, 2967
Ecl136II GAGCTC 1 cut(s) 2335
Eco130I CCWWGG 3 cut(s) 78, 3256, 3422
Eco147I AGGCCT 1 cut(s) 2170
Eco24I GRGCYC 5 cut(s) 587, 1070, 1287, 2225, 2337
Eco31I GGTCTC 4 cut(s) 1330, 1909, 2194, 3580
Eco32I GATATC 1 cut(s) 985
Eco47I GGWCC 4 cut(s) 840, 1754, 3443, 4006
Eco53kI GAGCTC 1 cut(s) 2335
Eco81I CCTNAGG 1 cut(s) 3591
Eco88I CYCGRG 3 cut(s) 309, 1645, 2065
EcoICRI GAGCTC 1 cut(s) 2335
EcoNI CCTNNNNNAGG 2 cut(s) 3594, 4311
EcoO109I RGGNCCY 4 cut(s) 583, 584, 1754, 3443
EcoRII CCWGG 9 cut(s) 241, 965, 1512, 1749, 1761, 1959, 2292, 3474, 4295
EcoRV GATATC 1 cut(s) 985
EcoT14I CCWWGG 3 cut(s) 78, 3256, 3422
EcoT22I ATGCAT 1 cut(s) 2478
EcoT38I GRGCYC 5 cut(s) 587, 1070, 1287, 2225, 2337
ErhI CCWWGG 3 cut(s) 78, 3256, 3422
FalI AAGNNNNNCTT 2 cut(s) 2233, 2265
FaqI GGGAC 3 cut(s) 326, 1767, 3025
FauI CCCGC 1 cut(s) 4346
FbaI TGATCA 4 cut(s) 1654, 1789, 2395, 3139
FriOI GRGCYC 5 cut(s) 587, 1070, 1287, 2225, 2337
FspBI CTAG 8 cut(s) 447, 501, 1149, 1209, 3215, 3698, 3854, 4365
GlaI GCGC 8 cut(s) 1382, 1823, 2651, 2675, 2738, 2762, 2786, 2936
GsaI CCCAGC 1 cut(s) 1461
GsuI CTGGAG 5 cut(s) 1159, 1299, 2084, 2624, 2897
HaeII RGCGCY 6 cut(s) 2653, 2677, 2740, 2764, 2788, 2938
HapII CCGG 4 cut(s) 553, 750, 779, 2163
HgaI GACGC 3 cut(s) 2507, 2885, 4283
HhaI GCGC 8 cut(s) 1383, 1824, 2652, 2676, 2739, 2763, 2787, 2937
Hin6I GCGC 8 cut(s) 1381, 1822, 2650, 2674, 2737, 2761, 2785, 2935
HinP1I GCGC 8 cut(s) 1381, 1822, 2650, 2674, 2737, 2761, 2785, 2935
HincII GTYRAC 2 cut(s) 355, 652
HindII GTYRAC 2 cut(s) 355, 652
HindIII AAGCTT 2 cut(s) 2367, 3826
HpaI GTTAAC 1 cut(s) 355
HpaII CCGG 4 cut(s) 553, 750, 779, 2163
HphI GGTGA 9 cut(s) 159, 713, 1388, 1877, 2681, 3464, 3764, 3986, 4058
Hpy166II GTNNAC 5 cut(s) 355, 652, 1613, 3251, 3689
Hpy8I GTNNAC 5 cut(s) 355, 652, 1613, 3251, 3689
Hpy99I CGWCG 3 cut(s) 363, 3780, 3918
HpyCH4III ACNGT 6 cut(s) 359, 770, 1579, 3776, 3974, 4041
HpyCH4IV ACGT 4 cut(s) 694, 1929, 2133, 3685
HpySE526I ACGT 4 cut(s) 694, 1929, 2133, 3685
HspAI GCGC 8 cut(s) 1381, 1822, 2650, 2674, 2737, 2761, 2785, 2935
Ksp22I TGATCA 4 cut(s) 1654, 1789, 2395, 3139
KspAI GTTAAC 1 cut(s) 355
MaeI CTAG 8 cut(s) 447, 501, 1149, 1209, 3215, 3698, 3854, 4365
MaeII ACGT 4 cut(s) 694, 1929, 2133, 3685
MbiI CCGCTC 1 cut(s) 4353
MfeI CAATTG 4 cut(s) 2630, 2939, 2966, 3053
MflI RGATCY 6 cut(s) 34, 1684, 3457, 3694, 3746, 4250
MhlI GDGCHC 6 cut(s) 587, 1070, 1287, 2225, 2337, 3253
MlsI TGGCCA 1 cut(s) 794
MluNI TGGCCA 1 cut(s) 794
MlyI GAGTC 5 cut(s) 134, 444, 599, 2003, 3577
Mox20I TGGCCA 1 cut(s) 794
Mph1103I ATGCAT 1 cut(s) 2478
MroXI GAANNNNTTC 1 cut(s) 874
MscI TGGCCA 1 cut(s) 794
MslI CAYNNNNRTG 3 cut(s) 172, 577, 3897
Msp20I TGGCCA 1 cut(s) 794
MspA1I CMGCKG 6 cut(s) 2210, 2626, 2731, 2869, 2890, 2911
MspCI CTTAAG 1 cut(s) 1222
MspI CCGG 4 cut(s) 553, 750, 779, 2163
MunI CAATTG 4 cut(s) 2630, 2939, 2966, 3053
Mva1269I GAATGC 2 cut(s) 340, 1543
MvaI CCWGG 9 cut(s) 243, 967, 1514, 1751, 1763, 1961, 2294, 3476, 4297
MvnI CGCG 1 cut(s) 711
NciI CCSGG 4 cut(s) 553, 751, 780, 2163
NlaIV GGNNCC 9 cut(s) 76, 584, 585, 756, 940, 1755, 3459, 3549, 4294
NmeAIII GCCGAG 1 cut(s) 857
NmuCI GTSAC 4 cut(s) 114, 165, 436, 2276
NruI TCGCGA 1 cut(s) 711
NsiI ATGCAT 1 cut(s) 2478
NspI RCATGY 3 cut(s) 185, 1046, 3487
PaeR7I CTCGAG 2 cut(s) 1645, 2065
PagI TCATGA 2 cut(s) 3136, 4309
PaqCI CACCTGC 1 cut(s) 476
PasI CCCWGGG 1 cut(s) 966
PceI AGGCCT 1 cut(s) 2170
PciI ACATGT 1 cut(s) 3483
PctI GAATGC 2 cut(s) 340, 1543
PdmI GAANNNNTTC 1 cut(s) 874
PflMI CCANNNNNTGG 1 cut(s) 736
PfoI TCCNGGA 3 cut(s) 551, 1959, 2161
PleI GAGTC 5 cut(s) 133, 443, 599, 2002, 3576
PpsI GAGTC 5 cut(s) 133, 443, 599, 2002, 3576
Ppu21I YACGTR 1 cut(s) 3686
PpuMI RGGWCCY 2 cut(s) 1754, 3443
PscI ACATGT 1 cut(s) 3483
PshAI GACNNNNGTC 1 cut(s) 3085
Psp124BI GAGCTC 1 cut(s) 2337
Psp5II RGGWCCY 2 cut(s) 1754, 3443
Psp6I CCWGG 9 cut(s) 241, 965, 1512, 1749, 1761, 1959, 2292, 3474, 4295
PspFI CCCAGC 1 cut(s) 1457
PspGI CCWGG 9 cut(s) 241, 965, 1512, 1749, 1761, 1959, 2292, 3474, 4295
PspN4I GGNNCC 9 cut(s) 76, 584, 585, 756, 940, 1755, 3459, 3549, 4294
PspOMI GGGCCC 1 cut(s) 583
PspPPI RGGWCCY 2 cut(s) 1754, 3443
PspXI VCTCGAGB 1 cut(s) 1645
PstI CTGCAG 3 cut(s) 1254, 2736, 2895
PstNI CAGNNNCTG 2 cut(s) 1409, 2890
PsuI RGATCY 6 cut(s) 34, 1684, 3457, 3694, 3746, 4250
PvuII CAGCTG 3 cut(s) 2210, 2731, 2890
RruI TCGCGA 1 cut(s) 711
RsaI GTAC 3 cut(s) 1318, 2105, 4101
RsaNI GTAC 3 cut(s) 1317, 2104, 4100
RseI CAYNNNNRTG 3 cut(s) 172, 577, 3897
SacI GAGCTC 1 cut(s) 2337
SchI GAGTC 5 cut(s) 134, 444, 599, 2003, 3577
SduI GDGCHC 6 cut(s) 587, 1070, 1287, 2225, 2337, 3253
Sfr274I CTCGAG 2 cut(s) 1645, 2065
SinI GGWCC 4 cut(s) 840, 1754, 3443, 4006
SlaI CTCGAG 2 cut(s) 1645, 2065
SmiMI CAYNNNNRTG 3 cut(s) 172, 577, 3897
SmlI CTYRAG 8 cut(s) 30, 629, 1222, 1645, 1737, 2047, 2065, 2364
SmoI CTYRAG 8 cut(s) 30, 629, 1222, 1645, 1737, 2047, 2065, 2364
SpeI ACTAGT 1 cut(s) 1208
SseBI AGGCCT 1 cut(s) 2170
SsiI CCGC 8 cut(s) 60, 63, 774, 2626, 2869, 2911, 2978, 4353
SspMI CTAG 8 cut(s) 447, 501, 1149, 1209, 3215, 3698, 3854, 4365
SstI GAGCTC 1 cut(s) 2337
StuI AGGCCT 1 cut(s) 2170
StyI CCWWGG 3 cut(s) 78, 3256, 3422
TaaI ACNGT 6 cut(s) 359, 770, 1579, 3776, 3974, 4041
TaiI ACGT 4 cut(s) 697, 1932, 2136, 3688
TaqI TCGA 9 cut(s) 466, 481, 610, 1017, 1646, 1770, 2066, 3063, 3079
TatI WGTACW 1 cut(s) 4099
TauI GCSGC 4 cut(s) 65, 776, 2629, 2914
TscAI CASTG 8 cut(s) 1159, 1254, 2024, 2188, 3110, 3874, 4398, 4405
TseFI GTSAC 4 cut(s) 114, 165, 436, 2276
Tsp45I GTSAC 4 cut(s) 114, 165, 436, 2276
TspGWI ACGGA 3 cut(s) 27, 1360, 3938
TspRI CASTG 8 cut(s) 1159, 1254, 2024, 2188, 3110, 3874, 4398, 4405
Van91I CCANNNNNTGG 1 cut(s) 736
Vha464I CTTAAG 1 cut(s) 1222
VneI GTGCAC 1 cut(s) 3249
VpaK11BI GGWCC 4 cut(s) 840, 1754, 3443, 4006
XagI CCTNNNNNAGG 2 cut(s) 3594, 4311
XapI RAATTY 8 cut(s) 522, 1500, 1558, 2233, 2600, 3623, 3996, 4177
XbaI TCTAGA 1 cut(s) 3697
XceI RCATGY 3 cut(s) 185, 1046, 3487
XcmI CCANNNNNNNNNTGG 2 cut(s) 802, 4267
XhoI CTCGAG 2 cut(s) 1645, 2065
XmnI GAANNNNTTC 1 cut(s) 874
XspI CTAG 8 cut(s) 447, 501, 1149, 1209, 3215, 3698, 3854, 4365
Zsp2I ATGCAT 1 cut(s) 2478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.