MD17G1002800.v1.1

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0003475
Physical Location & Seq
Reverse (-)
201838 .. 208895
7058 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1002800.v1.1.491

Sequence Viewer

Length: 4500 bp
ATGGCGCACGGAAAAGTCAGTCTCCCTCAAGATCTCCTCCCCTCGAATCTGGTCGATTCACACTTCTCCGCCAAAGATGAAGCCTTCCAAGGAAACATTGAGGAGAAGGCGCTAACGGGGTCCCTTGACATGTCAATTGGTGATCAAGTTGCCTCAGACAGCAGCATCCCTTTGTCTCCCCAGTGGCTGTATGCTAAACCAGATTCCAAGACACTGGCAACTGGAGCATCAGGAGAAATGCATGCAAATGATTCTCTGTCTCATGGAAACTCAACTGATCCCAATTCGAGAGACATTTGGCGTGTAGATGGATCTCAGGAGAAAAAAGATTGGAAGAGGAGTGCACCTGATTTAGATAGTAGCCGCCGCTGGCGTGAAGAGGAGAGAGAAACAGGCTTACTTGGTAGGAGAGATCGAAAGAAAGAAGATCGCCGTGTAGGTGCTAGTCTAACGAGGGATGCTACTGAGAATACAGCAGAAGATCGCCGTGTAGGTGCGACTTCAACTAAGGATGCTGCAGAAAATAAACTATTGTCTTCTGACCGCCGGAATGAATCCCGAAGAGATAACAAATGGTCATCAAGGTGGGGGCCTGAAGATAAAGACAAGGACTCTCGGATAGAGAAAAGGGCTGAGGTCGAGAGGGAAGATGTACATGCTGAGAAGCAGTCTTTTTCCAATAGCAACCGTGCAGCTTCTGAGCGTGATAGTGATTCTCGTGATAAGTGGAGGCCGCGCCATCGCATAGAGGTTCAACCAGCTGGGGCAGCCCCATATCATGCTGCACCAGGATTTGGGATGGCTCGGGGGCGTGTGGAAAAAGTAGGATTTGCTGCTGGACGAGGGAGGTCTAACACTGGATGCCTACAGACTGGCAAACCTGTTCTTGGAAAATCTGGCCATTTTGCTGATATGTATTGCTATCCCAGAGGAAAGCTCCTTGATATTTATCGCAAGCAAAAGAAGGCCTCAACTTTTGATATTATGCCTGATGGGATGGAGCATGTATCACCAATAACCCAAGTGGGCTCGATTGAGCCATTGGCTTTTGTTGCACCTGATGCAGAGGAAGAGGCTGGTCTGGGAGATATATTGAAGGGAAGAATTACTAGCAGTGAAGTGGTATACAACTCGTCTGGGGACAAGAATGTATTAAATGAAGATGCTAAAGGATCTAGCAATGTCACTTTAAGCAAGGAGGAAGGACACTTTGCAGCTAACCCAGAACAAAATGTCCAATCTGCCGAAAAGGTCATTTTGAACAATTCTTTTCCAGTTACTGTCGCTGAGGTGTCACCTATTTGTGGCTTACAAACACATATGTTAAAGGAATGTGTTGCTACTGAAGATGAGCAGAAAGTTCTGACGGTCACAGCAGCGGCAGATAGGGAGATTGGTCCTAGTAATGATGATGCTAACTCAAGAAGTTCAGGGTGCCAGGAGCAAACCTCAAGCAGTGATCATCACTATTTCAAGAGCAATGAGGACGCCCTTCTATTGGAAAGAATTGTCTCACCTGAGGATATGAGTTTGTGCTACCTTGATCCTCAAGGGAATATCCAGGGGCCTTTCCTGGGGATCGATATAATTGCATGGTTTGAGCAAGGATTTTTCGGAATTGACTTGCCAGTTCGTTCATTTGATGCTCCCAATGGATCTCCTTTCCAAGAACTTGGTGAAGTGATGCCACATCTGAAAACTAAATCCGGGCCTGTCTCCGACTCTAGTTTGCCTACTCAATTAGAGCCATTGGGTGCTGTTGGAGGCAGCTTGGACGAGAGAATATCTGCTCCAAACTATGATGGGGGGTCTGCTATTCTAAACAATCAAGGCATTCAGAATGTTGTTGCAGAAGATGAGGAAATTGTATTTCCTGGAAGGTCTAAAGGCAGCAGTGATTGCCTTTTAAGGTCTTCACCTGACATTCATGGCTCATTTGCAAATCCTCCCAGCCTTCATTCCCATGCAAATGAAGTTTTGGAAACTAACATCCCTGATCAGCAGGACGAAAAGCTGCATCCTTTTGGCCTGTTGATGTCAGAGCTCGGAGGCAACTCACTTTTAAGGCCTGCTCAATCATCTCATTCGTTTTTCGGCATGGATGATCAGGTTCCTTTTAGAGATACCTTCATTGAAGGTGCCACTGTTGCTAATCAGGATTCCCTTGGTGCCATGATTGATCAACCTTCCTTCGTGGAGACACGGCCTGATAATTATATAAAAAATAACACCAATGTTAGTCTGGGTTCTATAGATTCTCCCCATTTACCTCACATGGGGCAAGCAAGCAATGGCTTTGACCCAGCAGAACATATAATGTCACAGAAGTTACTAAAAGAACAGCTTCAGCAGCTGAACCATCTTTCTCCATTTACTGTTGCACATAGTGCAGGAACAGGAGTGGACCGGTTCCCTGGATTTGGTTTTTCTGACAGCAAGAACCCTAATATCCAGCCGTCATTCCATCATCCAGTGGCAGATATGGAACATATTTTTGAAATGCAGCATCAGCAGCAGCGTCAGTTGGAGCTTCGTCAGCAGCAACACGAGTTGGAGCTTCAGCAACAGCAGCGTAAGTTGGAGCGTCAGCAACAGCAGCGTTGGTTGGAGCTTCAGGAACACCAGCGTCAGTTGGAGCTTCAGGAACACCAACGTCAGTTGGAGATTCAGCGCCAGCATCAGTTGGAGCTGCAGCACCACCGTCAATTGGAGCTTCAGCAGCAGCAGCGTCAGCTGGAGCTTCAGCAACAGCGTCTCTTGCAGGATCAACTTCACCAACACCAAATGAATTTGCAGCAACAACAGAAATCTCAAGCTCAACACTTGCTCCTTGAACATTTTTTGCATCAACAGATGTCCGATTCTGGTTATGGGCAGTGGAAGGCAGATGCTACAAGAGGCAACCCTTTCGATCATTTACATTCAAGGAAGCACCTTCTAGATGACTTGCACCAGATGTCCCATTCCTCGAGGCATGATCCATTATTAGAGCACATCATCCAAGCAAATATAGGACGCCCGGGACAGACTGATTTCTTTGACCTGATATCTCAGGCCAAGCAAAGGAATATGCACACTTCGGAGCTGCAACCTCGTCTTCAGCAGCATGAGCTGCAGGCACAGCAGTTGTCCGCAGCATTGAGGGAGCAACTGCAGATGGAAGGGGAAAGGCGTATCGGTGCGAACCGGTTTGTAGATGAAGCAAGCCAAGTTGTTAGGGATCCTGTTGGTCATCACCAGGCTCAGATGGTAGGATTTAGTTCTTCAGAAAATTACCAGCAACTTCCCACCCATGAACCGCAACTAAGCCATCTCAACTGGAATCATGCCATACGGGAGCGACTCCAGGGAGGGTTCTACGAACCTAACTCTCTGGAATTTGAGAGGTCATCTGTTCCTGCTAGTTCGCTTGGGATGAACTTGGATATTCTCAGTGCACGTGGGCAGGGTCTGGATGAGTTTTATTCGTCTCATCCATTTGGTTTGGAGCACTTGCCTTTTGGGAACAATGGACATCTGGAAGATGGCTCCGCTGAAGCACTTGTACCGCATTCGCATCTTCATGCCGAGCAAACCCGCCGAGACTCAGAAGCTGCCATGGATTTTTCTGATGCAAATATATGGTCTTCATCCGATGGTGATAAAGAACCCTCAAAACAAATTTTTATGGACCTTCACCAAAAAATCGGTCGTCAATCTACACGGTTATCAGAAGTTGATTATCAGCATCACCTATCATCTTCTAGAAACCGGGGAGAATCTGTACATCATCCATTTAGTCTTCTCCCTGATCAAGCAGTTGGTATGAACAACTCTTTCACGGAAGGGCCCTTTACATCTAATCCCAGAGCATTTATGGAAGATTTATCAGAGTTGGAGGGAAATAATTGGAAGAAGCAGGTTGTGAAAAGCTATGAAGGCAACATGGTAGAACAAGAAGAAACATCAAGGGAACTTCATAGTAATGCCCATAGCAGGCAGAGTTCACTTAGCAGTGCTGGGGGAGGTGGGAGTTTTTACAGCTCCGAGACAGGAATAGATAAACCGCTTGGAGAAGAGATTTCTAATGGGAGGCTGCCTTCTACTGTAACCAAAGGGTACGACAATGCTTTGCATAGACGCCGGGTTTTATCTTCCCAGGACGTTTTGTCTGAGGCAGCACTTTCTTTGCCTGTCAAACAAAGAAATCCAGCTGCAACCCAGGTCTCTGAAACGCAAGCATCTAGCAAGAATGATGCGCAATTTAGGAGGACTTCGTCTTACAGTGACGCTACAGTGTCCGAGGCATCATTCATAGACATGCTCAAGAAACCAGTCATTTCAGAGGCTGATGCAGCCAACAGAGCAGTGGCACTGGAGTCATCTGATGGAGGTGGCCAAGCGGGGCGGACTGGCAAGAAGAAAGGGAAGAAGGGAAGACAGATTGATCCTGCTCTCCTCGGCTTTAAGGTTTCCAGCAACCGGATCTTGATGGGCGAGATCCATCGCCTCAATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1500

Amino Acids

167.04

Weight (kDa)

5.66

Isoelectric Point (pI)

59.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 513 - 547 1.4e-09 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 2968
Acc16I TGCGCA 1 cut(s) 4239
Acc36I ACCTGC 1 cut(s) 3889
AccB1I GGYRCC 3 cut(s) 1434, 2138, 2168
AccB7I CCANNNNNTGG 1 cut(s) 794
AccI GTMKAC 1 cut(s) 1125
AccII CGCG 1 cut(s) 736
AcoI YGGCCR 2 cut(s) 898, 4375
AcsI RAATTY 3 cut(s) 2788, 3377, 3660
AcvI CACGTG 1 cut(s) 3440
AcyI GRCGYC 3 cut(s) 1488, 3016, 4120
AdeI CACNNNGTG 1 cut(s) 2387
AfaI GTAC 4 cut(s) 654, 3546, 3765, 4100
AflIII ACRYGT 1 cut(s) 129
AgeI ACCGGT 2 cut(s) 2406, 3186
AgsI TTSAA 9 cut(s) 504, 755, 1096, 1261, 1474, 2135, 2498, 2834, 2925
AhdI GACNNNNNGTC 1 cut(s) 4147
AloI GAACNNNNNNTCC 2 cut(s) 1218, 1250
Alw21I GWGCWC 5 cut(s) 346, 2046, 2994, 3439, 3492
Alw44I GTGCAC 2 cut(s) 342, 3435
AlwNI CAGNNNCTG 7 cut(s) 187, 698, 1280, 2353, 3451, 3593, 4328
Ama87I CYCGRG 3 cut(s) 804, 2968, 3019
ApaI GGGCCC 1 cut(s) 3831
ApaLI GTGCAC 2 cut(s) 342, 3435
ApoI RAATTY 3 cut(s) 2788, 3377, 3660
ArsI GACNNNNNNTTYG 4 cut(s) 3608, 3640, 3673, 3705
AsiGI ACCGGT 2 cut(s) 2406, 3186
Asp700I GAANNNNTTC 1 cut(s) 2343
AspLEI GCGC 5 cut(s) 7, 112, 738, 2673, 4240
AspS9I GGNCC 9 cut(s) 120, 590, 1397, 1565, 1709, 2404, 3670, 3827, 3828
AsuC2I CCSGG 5 cut(s) 1708, 3020, 3021, 3752, 4124
AvaI CYCGRG 3 cut(s) 804, 2968, 3019
AvaII GGWCC 4 cut(s) 120, 1397, 2404, 3670
AxyI CCTNAGG 1 cut(s) 1518
BaeGI GKGCMC 3 cut(s) 346, 3439, 3831
BalI TGGCCA 2 cut(s) 900, 4377
BamHI GGATCC 1 cut(s) 3220
BanI GGYRCC 3 cut(s) 1434, 2138, 2168
BanII GRGCYC 3 cut(s) 1031, 2046, 3831
BarI GAAGNNNNNNTAC 2 cut(s) 3528, 3560
BauI CACGAG 2 cut(s) 717, 2546
BbrPI CACGTG 1 cut(s) 3440
BbsI GAAGAC 6 cut(s) 528, 1905, 3089, 3618, 3773, 4423
Bbv12I GWGCWC 5 cut(s) 346, 2046, 2994, 3439, 3492
BbvCI CCTCAGC 2 cut(s) 633, 1287
BceAI ACGGC 4 cut(s) 417, 471, 2219, 2440
BcgI CGANNNNNNTGC 4 cut(s) 1571, 1605, 2890, 2924
BclI TGATCA 6 cut(s) 142, 1459, 1996, 2104, 2179, 3790
BcnI CCSGG 5 cut(s) 1708, 3020, 3021, 3752, 4124
BfaI CTAG 9 cut(s) 444, 1110, 1176, 1401, 1725, 2939, 3402, 3744, 4224
BfmI CTRYAG 7 cut(s) 516, 866, 2250, 2690, 3113, 3152, 4272
BfoI RGCGCY 2 cut(s) 113, 2674
BfuAI ACCTGC 1 cut(s) 3889
BglII AGATCT 1 cut(s) 31
Bme18I GGWCC 4 cut(s) 120, 1397, 2404, 3670
BmeRI GACNNNNNGTC 1 cut(s) 4147
BmeT110I CYCGRG 3 cut(s) 804, 2968, 3019
BmgT120I GGNCC 9 cut(s) 120, 590, 1397, 1565, 1709, 2404, 3670, 3827, 3828
BmrI ACTGGG 1 cut(s) 175
BmuI ACTGGG 1 cut(s) 175
BpiI GAAGAC 6 cut(s) 528, 1905, 3089, 3618, 3773, 4423
BplI GAGNNNNNCTC 4 cut(s) 921, 953, 1433, 1465
BpmI CTGGAG 4 cut(s) 243, 2756, 3329, 4376
Bpu10I CCTNAGC 2 cut(s) 633, 1287
BpuEI CTTGAG 6 cut(s) 12, 1405, 1435, 1533, 2796, 4289
BpuMI CCSGG 5 cut(s) 1708, 3020, 3021, 3752, 4124
Bsa29I ATCGAT 1 cut(s) 1581
BsaAI YACGTR 1 cut(s) 3440
BsaBI GATNNNNATC 1 cut(s) 948
BsaHI GRCGYC 3 cut(s) 1488, 3016, 4120
BsaI GGTCTC 1 cut(s) 4210
BsaWI WCCGGW 3 cut(s) 2406, 3186, 4461
BsaXI ACNNNNNCTCC 4 cut(s) 2811, 2841, 3747, 3777
Bse118I RCCGGY 2 cut(s) 2406, 3186
Bse21I CCTNAGG 1 cut(s) 1518
Bse3DI GCAATG 3 cut(s) 1186, 1486, 2296
Bse8I GATNNNNATC 1 cut(s) 948
BseCI ATCGAT 1 cut(s) 1581
BseJI GATNNNNATC 1 cut(s) 948
BseMI GCAATG 3 cut(s) 1186, 1486, 2296
BseRI GAGGAG 5 cut(s) 26, 116, 352, 395, 4427
BseSI GKGCMC 3 cut(s) 346, 3439, 3831
BseYI CCCAGC 4 cut(s) 761, 1949, 2302, 3998
BsgI GTGCAG 3 cut(s) 711, 768, 2409
Bsh1236I CGCG 1 cut(s) 736
Bsh1285I CGRYCG 1 cut(s) 3691
BshNI GGYRCC 3 cut(s) 1434, 2138, 2168
BshTI ACCGGT 2 cut(s) 2406, 3186
BshVI ATCGAT 1 cut(s) 1581
BsiEI CGRYCG 1 cut(s) 3691
BsiHKAI GWGCWC 5 cut(s) 346, 2046, 2994, 3439, 3492
BsiHKCI CYCGRG 3 cut(s) 804, 2968, 3019
BsiSI CCGG 8 cut(s) 547, 1707, 2407, 3020, 3187, 3751, 4123, 4462
BslFI GGGAC 4 cut(s) 106, 1154, 2944, 3036
BsmBI CGTCTC 2 cut(s) 2759, 3474
BsmFI GGGAC 4 cut(s) 106, 1154, 2944, 3036
BsmI GAATGC 2 cut(s) 1833, 3550
Bso31I GGTCTC 1 cut(s) 4210
BsoBI CYCGRG 3 cut(s) 804, 2968, 3019
Bsp120I GGGCCC 1 cut(s) 3827
Bsp1286I GDGCHC 7 cut(s) 346, 1031, 2046, 2994, 3439, 3492, 3831
Bsp1407I TGTACA 2 cut(s) 652, 3763
Bsp19I CCATGG 1 cut(s) 3597
BspDI ATCGAT 1 cut(s) 1581
BspFNI CGCG 1 cut(s) 736
BspMAI CTGCAG 4 cut(s) 520, 2694, 3117, 3156
BspMI ACCTGC 1 cut(s) 3889
BspT107I GGYRCC 3 cut(s) 1434, 2138, 2168
BspTNI GGTCTC 1 cut(s) 4210
BsrDI GCAATG 3 cut(s) 1186, 1486, 2296
BsrFI RCCGGY 2 cut(s) 2406, 3186
BsrGI TGTACA 2 cut(s) 652, 3763
BssAI RCCGGY 2 cut(s) 2406, 3186
BssNAI GTATAC 1 cut(s) 1126
BssNI GRCGYC 3 cut(s) 1488, 3016, 4120
BssSI CACGAG 2 cut(s) 717, 2546
BssT1I CCWWGG 3 cut(s) 88, 2164, 3597
Bst1107I GTATAC 1 cut(s) 1126
Bst2BI CACGAG 2 cut(s) 717, 2546
Bst6I CTCTTC 5 cut(s) 329, 372, 556, 1065, 4050
BstACI GRCGYC 3 cut(s) 1488, 3016, 4120
BstAPI GCANNNNNTGC 4 cut(s) 1061, 1899, 2387, 3112
BstAUI TGTACA 2 cut(s) 652, 3763
BstBAI YACGTR 1 cut(s) 3440
BstDSI CCRYGG 1 cut(s) 3597
BstFNI CGCG 1 cut(s) 736
BstH2I RGCGCY 2 cut(s) 113, 2674
BstHHI GCGC 5 cut(s) 7, 112, 738, 2673, 4240
BstMCI CGRYCG 1 cut(s) 3691
BstNSI RCATGY 5 cut(s) 133, 245, 659, 1007, 4303
BstSFI CTRYAG 7 cut(s) 516, 866, 2250, 2690, 3113, 3152, 4272
BstSLI GKGCMC 3 cut(s) 346, 3439, 3831
BstUI CGCG 1 cut(s) 736
BstV2I GAAGAC 6 cut(s) 528, 1905, 3089, 3618, 3773, 4423
BstX2I RGATCY 7 cut(s) 31, 311, 1172, 1655, 3220, 4464, 4479
BstXI CCANNNNNNTGG 2 cut(s) 214, 1673
BstYI RGATCY 7 cut(s) 31, 311, 1172, 1655, 3220, 4464, 4479
BstZ17I GTATAC 1 cut(s) 1126
Bsu15I ATCGAT 1 cut(s) 1581
Bsu36I CCTNAGG 1 cut(s) 1518
BsuTUI ATCGAT 1 cut(s) 1581
BtgI CCRYGG 1 cut(s) 3597
BtgZI GCGATG 2 cut(s) 725, 4469
BtsI GCAGTG 6 cut(s) 1120, 1462, 1900, 2882, 4000, 4353
BveI ACCTGC 1 cut(s) 3889
CaiI CAGNNNCTG 7 cut(s) 187, 698, 1280, 2353, 3451, 3593, 4328
CfoI GCGC 5 cut(s) 7, 112, 738, 2673, 4240
Cfr10I RCCGGY 2 cut(s) 2406, 3186
Cfr13I GGNCC 9 cut(s) 120, 590, 1397, 1565, 1709, 2404, 3670, 3827, 3828
Cfr9I CCCGGG 1 cut(s) 3019
ClaI ATCGAT 1 cut(s) 1581
CseI GACGC 9 cut(s) 1496, 2507, 2573, 2615, 2717, 2741, 3024, 4128, 4277
Csp6I GTAC 4 cut(s) 653, 3545, 3764, 4099
CspAI ACCGGT 2 cut(s) 2406, 3186
CviQI GTAC 4 cut(s) 653, 3545, 3764, 4099
DraIII CACNNNGTG 1 cut(s) 2387
DriI GACNNNNNGTC 1 cut(s) 4147
EaeI YGGCCR 2 cut(s) 898, 4375
Eam1104I CTCTTC 5 cut(s) 329, 372, 556, 1065, 4050
Eam1105I GACNNNNNGTC 1 cut(s) 4147
EarI CTCTTC 5 cut(s) 329, 372, 556, 1065, 4050
EciI GGCGGA 2 cut(s) 58, 4402
Ecl136II GAGCTC 1 cut(s) 2044
Eco130I CCWWGG 3 cut(s) 88, 2164, 3597
Eco147I AGGCCT 2 cut(s) 968, 2068
Eco24I GRGCYC 3 cut(s) 1031, 2046, 3831
Eco31I GGTCTC 1 cut(s) 4210
Eco32I GATATC 1 cut(s) 3048
Eco47I GGWCC 4 cut(s) 120, 1397, 2404, 3670
Eco53kI GAGCTC 1 cut(s) 2044
Eco72I CACGTG 1 cut(s) 3440
Eco81I CCTNAGG 1 cut(s) 1518
Eco88I CYCGRG 3 cut(s) 804, 2968, 3019
EcoICRI GAGCTC 1 cut(s) 2044
EcoO109I RGGNCCY 5 cut(s) 120, 590, 1565, 3827, 3828
EcoRV GATATC 1 cut(s) 3048
EcoT14I CCWWGG 3 cut(s) 88, 2164, 3597
EcoT22I ATGCAT 1 cut(s) 243
EcoT38I GRGCYC 3 cut(s) 1031, 2046, 3831
ErhI CCWWGG 3 cut(s) 88, 2164, 3597
Esp3I CGTCTC 2 cut(s) 2759, 3474
FalI AAGNNNNNCTT 2 cut(s) 2328, 2360
FaqI GGGAC 4 cut(s) 106, 1154, 2944, 3036
FauI CCCGC 2 cut(s) 3584, 4375
FauNDI CATATG 1 cut(s) 1320
FbaI TGATCA 6 cut(s) 142, 1459, 1996, 2104, 2179, 3790
FblI GTMKAC 1 cut(s) 1125
FriOI GRGCYC 3 cut(s) 1031, 2046, 3831
FspBI CTAG 9 cut(s) 444, 1110, 1176, 1401, 1725, 2939, 3402, 3744, 4224
FspI TGCGCA 1 cut(s) 4239
GlaI GCGC 5 cut(s) 6, 111, 737, 2672, 4239
GsaI CCCAGC 4 cut(s) 765, 1953, 2306, 4002
GsuI CTGGAG 4 cut(s) 243, 2756, 3329, 4376
HaeII RGCGCY 2 cut(s) 113, 2674
HapII CCGG 8 cut(s) 547, 1707, 2407, 3020, 3187, 3751, 4123, 4462
HgaI GACGC 9 cut(s) 1496, 2507, 2573, 2615, 2717, 2741, 3024, 4128, 4277
HhaI GCGC 5 cut(s) 7, 112, 738, 2673, 4240
Hin1I GRCGYC 3 cut(s) 1488, 3016, 4120
Hin6I GCGC 5 cut(s) 5, 110, 736, 2671, 4238
HinP1I GCGC 5 cut(s) 5, 110, 736, 2671, 4238
HpaII CCGG 8 cut(s) 547, 1707, 2407, 3020, 3187, 3751, 4123, 4462
Hpy166II GTNNAC 5 cut(s) 344, 1126, 2404, 3437, 3986
Hpy8I GTNNAC 5 cut(s) 344, 1126, 2404, 3437, 3986
HpyCH4IV ACGT 3 cut(s) 2653, 3439, 4143
HpySE526I ACGT 3 cut(s) 2653, 3439, 4143
Hsp92I GRCGYC 3 cut(s) 1488, 3016, 4120
HspAI GCGC 5 cut(s) 5, 110, 736, 2671, 4238
KflI GGGWCCC 1 cut(s) 120
Ksp22I TGATCA 6 cut(s) 142, 1459, 1996, 2104, 2179, 3790
MaeI CTAG 9 cut(s) 444, 1110, 1176, 1401, 1725, 2939, 3402, 3744, 4224
MaeII ACGT 3 cut(s) 2653, 3439, 4143
MaeIII GTNAC 8 cut(s) 1183, 1276, 1293, 1369, 2319, 2328, 4087, 4265
MfeI CAATTG 2 cut(s) 135, 2705
MflI RGATCY 7 cut(s) 31, 311, 1172, 1655, 3220, 4464, 4479
MhlI GDGCHC 7 cut(s) 346, 1031, 2046, 2994, 3439, 3492, 3831
MlsI TGGCCA 2 cut(s) 900, 4377
MluNI TGGCCA 2 cut(s) 900, 4377
MlyI GAGTC 5 cut(s) 605, 1715, 3336, 3578, 4367
Mox20I TGGCCA 2 cut(s) 900, 4377
Mph1103I ATGCAT 1 cut(s) 243
MroXI GAANNNNTTC 1 cut(s) 2343
MscI TGGCCA 2 cut(s) 900, 4377
MseI TTAA 6 cut(s) 1154, 1190, 1325, 1907, 2063, 4446
MslI CAYNNNNRTG 7 cut(s) 246, 583, 1962, 1968, 3561, 3963, 4298
Msp20I TGGCCA 2 cut(s) 900, 4377
MspA1I CMGCKG 7 cut(s) 369, 761, 1379, 2353, 2734, 3533, 4193
MspI CCGG 8 cut(s) 547, 1707, 2407, 3020, 3187, 3751, 4123, 4462
MunI CAATTG 2 cut(s) 135, 2705
Mva1269I GAATGC 2 cut(s) 1833, 3550
MvnI CGCG 1 cut(s) 736
NciI CCSGG 5 cut(s) 1708, 3020, 3021, 3752, 4124
NcoI CCATGG 1 cut(s) 3597
NdeI CATATG 1 cut(s) 1320
NmeAIII GCCGAG 3 cut(s) 3592, 3605, 4419
NmuCI GTSAC 5 cut(s) 1183, 1293, 1369, 2319, 4265
NsbI TGCGCA 1 cut(s) 4239
NsiI ATGCAT 1 cut(s) 243
NspI RCATGY 5 cut(s) 133, 245, 659, 1007, 4303
PaeI GCATGC 1 cut(s) 245
PaeR7I CTCGAG 1 cut(s) 2968
PceI AGGCCT 2 cut(s) 968, 2068
PciI ACATGT 1 cut(s) 129
PctI GAATGC 2 cut(s) 1833, 3550
PdmI GAANNNNTTC 1 cut(s) 2343
PflFI GACNNNGTC 1 cut(s) 4255
PflMI CCANNNNNTGG 1 cut(s) 794
PfoI TCCNGGA 1 cut(s) 1873
PinAI ACCGGT 2 cut(s) 2406, 3186
PleI GAGTC 5 cut(s) 605, 1715, 3336, 3578, 4366
PmaCI CACGTG 1 cut(s) 3440
PmlI CACGTG 1 cut(s) 3440
PpsI GAGTC 5 cut(s) 605, 1715, 3336, 3578, 4366
Ppu21I YACGTR 1 cut(s) 3440
PpuMI RGGWCCY 1 cut(s) 120
PscI ACATGT 1 cut(s) 129
Psp124BI GAGCTC 1 cut(s) 2046
Psp5II RGGWCCY 1 cut(s) 120
PspCI CACGTG 1 cut(s) 3440
PspFI CCCAGC 4 cut(s) 761, 1949, 2302, 3998
PspOMI GGGCCC 1 cut(s) 3827
PspPI GGNCC 9 cut(s) 120, 590, 1397, 1565, 1709, 2404, 3670, 3827, 3828
PspPPI RGGWCCY 1 cut(s) 120
PspXI VCTCGAGB 1 cut(s) 2968
PstI CTGCAG 4 cut(s) 520, 2694, 3117, 3156
PstNI CAGNNNCTG 7 cut(s) 187, 698, 1280, 2353, 3451, 3593, 4328
PsuI RGATCY 7 cut(s) 31, 311, 1172, 1655, 3220, 4464, 4479
PsyI GACNNNGTC 1 cut(s) 4255
PvuII CAGCTG 4 cut(s) 761, 2353, 2734, 4193
RsaI GTAC 4 cut(s) 654, 3546, 3765, 4100
RsaNI GTAC 4 cut(s) 653, 3545, 3764, 4099
RseI CAYNNNNRTG 7 cut(s) 246, 583, 1962, 1968, 3561, 3963, 4298
SacI GAGCTC 1 cut(s) 2046
SaqAI TTAA 6 cut(s) 1154, 1190, 1325, 1907, 2063, 4446
Sau96I GGNCC 9 cut(s) 120, 590, 1397, 1565, 1709, 2404, 3670, 3827, 3828
SchI GAGTC 5 cut(s) 605, 1715, 3336, 3578, 4367
SduI GDGCHC 7 cut(s) 346, 1031, 2046, 2994, 3439, 3492, 3831
SfcI CTRYAG 7 cut(s) 516, 866, 2250, 2690, 3113, 3152, 4272
Sfr274I CTCGAG 1 cut(s) 2968
SinI GGWCC 4 cut(s) 120, 1397, 2404, 3670
SlaI CTCGAG 1 cut(s) 2968
SmaI CCCGGG 1 cut(s) 3021
SmiMI CAYNNNNRTG 7 cut(s) 246, 583, 1962, 1968, 3561, 3963, 4298
SmlI CTYRAG 7 cut(s) 27, 1420, 1450, 1548, 2811, 2968, 4304
SmoI CTYRAG 7 cut(s) 27, 1420, 1450, 1548, 2811, 2968, 4304
SphI GCATGC 1 cut(s) 245
SseBI AGGCCT 2 cut(s) 968, 2068
SspMI CTAG 9 cut(s) 444, 1110, 1176, 1401, 1725, 2939, 3402, 3744, 4224
SstI GAGCTC 1 cut(s) 2046
StuI AGGCCT 2 cut(s) 968, 2068
StyI CCWWGG 3 cut(s) 88, 2164, 3597
TaiI ACGT 3 cut(s) 2656, 3442, 4146
TaqI TCGA 9 cut(s) 44, 54, 287, 415, 639, 1031, 1581, 2910, 2969
TaqII GACCGA 1 cut(s) 3677
TatI WGTACW 2 cut(s) 652, 3763
TauI GCSGC 4 cut(s) 366, 369, 736, 1382
Tru1I TTAA 6 cut(s) 1154, 1190, 1325, 1907, 2063, 4446
Tru9I TTAA 6 cut(s) 1154, 1190, 1325, 1907, 2063, 4446
TseFI GTSAC 5 cut(s) 1183, 1293, 1369, 2319, 4265
Tsp45I GTSAC 5 cut(s) 1183, 1293, 1369, 2319, 4265
TspGWI ACGGA 2 cut(s) 24, 3836
TspMI CCCGGG 1 cut(s) 3019
Tth111I GACNNNGTC 1 cut(s) 4255
Van91I CCANNNNNTGG 1 cut(s) 794
VneI GTGCAC 2 cut(s) 342, 3435
VpaK11BI GGWCC 4 cut(s) 120, 1397, 2404, 3670
XapI RAATTY 3 cut(s) 2788, 3377, 3660
XbaI TCTAGA 2 cut(s) 2938, 3743
XceI RCATGY 5 cut(s) 133, 245, 659, 1007, 4303
XcmI CCANNNNNNNNNTGG 3 cut(s) 2239, 3853, 4345
XhoI CTCGAG 1 cut(s) 2968
XmaI CCCGGG 1 cut(s) 3019
XmiI GTMKAC 1 cut(s) 1125
XmnI GAANNNNTTC 1 cut(s) 2343
XspI CTAG 9 cut(s) 444, 1110, 1176, 1401, 1725, 2939, 3402, 3744, 4224
Zsp2I ATGCAT 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.