Rroxscaffold_2G00076860

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003475
Physical Location & Seq
Forward (+)
567942 .. 575758
7817 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00076860.1

Sequence Viewer

Length: 537 bp
ATGGTTCAGGTGGATGTGGGGGTTTTTCACAGCTCTGAGATGGGATTAGATAAATCACTTGGAGAAGATGTTTCTGATGGGAGGCAGCCTTCTACTATAACTAAAGGGTTTGACAGTGCTACACAGAGACGCCGCGTCTTATCTTCACAGGATGTTTCGTCTGAGTCTGCACTCTCTCTGCCTGTCAAGCCGAGAAGCTCCATAAGCCTCAGAACTTCTGAAAAAAATCCTGCTGCCACACTGGTCTCTGAATCTCGGGCATCCAGGAAAAATGATGCACAGTTTAGGAGGACTTCATCTTACAGTGATGCTGGTGTCTCTGAGACATCCTTCATAGATGTGCTCAAGAAACCAGCCATTCCAGAGGCTGATGTAGCCAACAGGGGTGCAGTGGAGTCATCTGATGGAGGTGGCCAAGTGGGGCGAACTGGCAAGAAGAAAGGCAAGAAAGGAAGACAGATCGATCCTGCTCTCCTTGGCTTTAAGGTATCCAGCAACCGGATCATGATGGGTGAGATCCATCGCCTGGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

178

Amino Acids

19.08

Weight (kDa)

9.59

Isoelectric Point (pI)

47.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 526
AccII CGCG 1 cut(s) 135
AciI CCGC 1 cut(s) 133
AclWI GGATC 3 cut(s) 458, 509, 511
AcoI YGGCCR 1 cut(s) 412
AcyI GRCGYC 1 cut(s) 130
AfiI CCNNNNNNNGG 2 cut(s) 498, 526
AhdI GACNNNNNGTC 1 cut(s) 134
AjnI CCWGG 2 cut(s) 263, 525
AluBI AGCT 2 cut(s) 33, 198
AluI AGCT 2 cut(s) 33, 198
Alw21I GWGCWC 1 cut(s) 345
Alw26I GTCTC 4 cut(s) 121, 250, 317, 322
AlwI GGATC 3 cut(s) 458, 509, 511
AlwNI CAGNNNCTG 1 cut(s) 368
Ama87I CYCGRG 1 cut(s) 255
AoxI GGCC 1 cut(s) 412
ApeKI GCWGC 2 cut(s) 85, 233
AsuHPI GGTGA 1 cut(s) 524
AvaI CYCGRG 1 cut(s) 255
BalI TGGCCA 1 cut(s) 414
BbsI GAAGAC 1 cut(s) 460
Bbv12I GWGCWC 1 cut(s) 345
BbvI GCAGC 2 cut(s) 97, 220
BccI CCATC 5 cut(s) 34, 71, 398, 502, 528
BciT130I CCWGG 2 cut(s) 265, 527
BciVI GTATCC 1 cut(s) 499
BcoDI GTCTC 4 cut(s) 121, 250, 317, 322
BfuI GTATCC 1 cut(s) 499
BisI GCNGC 3 cut(s) 86, 133, 234
BlsI GCNGC 3 cut(s) 87, 134, 235
Bme1390I CCNGG 2 cut(s) 265, 527
BmeRI GACNNNNNGTC 1 cut(s) 134
BmeT110I CYCGRG 1 cut(s) 255
BmrFI CCNGG 2 cut(s) 265, 527
BmsI GCATC 3 cut(s) 265, 269, 298
BpiI GAAGAC 1 cut(s) 460
BpuEI CTTGAG 1 cut(s) 329
Bsa29I ATCGAT 1 cut(s) 462
BsaHI GRCGYC 1 cut(s) 130
BsaI GGTCTC 1 cut(s) 250
BsaJI CCNNGG 1 cut(s) 475
BsaWI WCCGGW 1 cut(s) 498
BsaXI ACNNNNNCTCC 2 cut(s) 54, 84
Bsc4I CCNNNNNNNGG 2 cut(s) 498, 526
Bse1I ACTGG 2 cut(s) 246, 433
BseBI CCWGG 2 cut(s) 265, 527
BseCI ATCGAT 1 cut(s) 462
BseDI CCNNGG 1 cut(s) 475
BseGI GGATG 5 cut(s) 19, 157, 260, 326, 535
BseLI CCNNNNNNNGG 2 cut(s) 498, 526
BseMII CTCAG 4 cut(s) 27, 153, 223, 312
BseNI ACTGG 2 cut(s) 246, 433
BseXI GCAGC 2 cut(s) 97, 220
BsgI GTGCAG 2 cut(s) 153, 408
Bsh1236I CGCG 1 cut(s) 135
BshFI GGCC 1 cut(s) 414
BshVI ATCGAT 1 cut(s) 462
BsiHKAI GWGCWC 1 cut(s) 345
BsiHKCI CYCGRG 1 cut(s) 255
BsiSI CCGG 1 cut(s) 499
BslI CCNNNNNNNGG 2 cut(s) 498, 526
BsmAI GTCTC 4 cut(s) 121, 250, 317, 322
BsmBI CGTCTC 1 cut(s) 121
BsnI GGCC 1 cut(s) 414
Bso31I GGTCTC 1 cut(s) 250
BsoBI CYCGRG 1 cut(s) 255
Bsp1286I GDGCHC 1 cut(s) 345
Bsp143I GATC 4 cut(s) 459, 463, 501, 516
BspACI CCGC 1 cut(s) 133
BspANI GGCC 1 cut(s) 414
BspCNI CTCAG 4 cut(s) 28, 154, 222, 313
BspDI ATCGAT 1 cut(s) 462
BspFNI CGCG 1 cut(s) 135
BspHI TCATGA 1 cut(s) 504
BspPI GGATC 3 cut(s) 458, 509, 511
BspTNI GGTCTC 1 cut(s) 250
BsrI ACTGG 2 cut(s) 246, 433
BssECI CCNNGG 1 cut(s) 475
BssMI GATC 4 cut(s) 459, 463, 501, 516
BssNI GRCGYC 1 cut(s) 130
BssT1I CCWWGG 1 cut(s) 475
Bst2UI CCWGG 2 cut(s) 265, 527
Bst4CI ACNGT 3 cut(s) 116, 282, 305
BstACI GRCGYC 1 cut(s) 130
BstDEI CTNAG 4 cut(s) 36, 162, 209, 321
BstF5I GGATG 5 cut(s) 19, 157, 260, 326, 535
BstFNI CGCG 1 cut(s) 135
BstKTI GATC 4 cut(s) 462, 466, 504, 519
BstMAI GTCTC 4 cut(s) 121, 250, 317, 322
BstMBI GATC 4 cut(s) 459, 463, 501, 516
BstMWI GCNNNNNNNGC 3 cut(s) 187, 204, 374
BstNI CCWGG 2 cut(s) 265, 527
BstSCI CCNGG 2 cut(s) 263, 525
BstUI CGCG 1 cut(s) 135
BstV1I GCAGC 2 cut(s) 97, 220
BstV2I GAAGAC 1 cut(s) 460
BstX2I RGATCY 1 cut(s) 516
BstYI RGATCY 1 cut(s) 516
Bsu15I ATCGAT 1 cut(s) 462
BsuI GTATCC 1 cut(s) 499
BsuRI GGCC 1 cut(s) 414
BsuTUI ATCGAT 1 cut(s) 462
BtgZI GCGATG 1 cut(s) 506
BtsCI GGATG 5 cut(s) 19, 157, 260, 326, 535
BtsI GCAGTG 1 cut(s) 396
BtsIMutI CAGTG 4 cut(s) 121, 239, 310, 396
CaiI CAGNNNCTG 1 cut(s) 368
CciI TCATGA 1 cut(s) 504
ClaI ATCGAT 1 cut(s) 462
CseI GACGC 2 cut(s) 124, 138
CviAII CATG 1 cut(s) 505
DdeI CTNAG 4 cut(s) 36, 162, 209, 321
DpnI GATC 4 cut(s) 461, 465, 503, 518
DpnII GATC 4 cut(s) 459, 463, 501, 516
DriI GACNNNNNGTC 1 cut(s) 134
EaeI YGGCCR 1 cut(s) 412
Eam1105I GACNNNNNGTC 1 cut(s) 134
Eco130I CCWWGG 1 cut(s) 475
Eco31I GGTCTC 1 cut(s) 250
Eco88I CYCGRG 1 cut(s) 255
EcoRII CCWGG 2 cut(s) 263, 525
EcoT14I CCWWGG 1 cut(s) 475
ErhI CCWWGG 1 cut(s) 475
Esp3I CGTCTC 1 cut(s) 121
FaeI CATG 1 cut(s) 508
FaiI YATR 4 cut(s) 98, 203, 335, 506
FatI CATG 1 cut(s) 504
Fnu4HI GCNGC 3 cut(s) 86, 133, 234
FokI GGATG 4 cut(s) 26, 164, 247, 313
Fsp4HI GCNGC 3 cut(s) 86, 133, 234
GluI GCNGC 3 cut(s) 86, 133, 234
HaeIII GGCC 1 cut(s) 414
HapII CCGG 1 cut(s) 499
HgaI GACGC 2 cut(s) 124, 138
Hin1I GRCGYC 1 cut(s) 130
Hin1II CATG 1 cut(s) 508
HinfI GANTC 3 cut(s) 164, 251, 395
HpaII CCGG 1 cut(s) 499
HphI GGTGA 1 cut(s) 524
Hpy188I TCNGA 8 cut(s) 37, 76, 163, 212, 220, 250, 322, 403
Hpy188III TCNNGA 3 cut(s) 346, 362, 505
HpyAV CCTTC 2 cut(s) 99, 340
HpyCH4III ACNGT 3 cut(s) 116, 282, 305
HpyCH4V TGCA 3 cut(s) 170, 278, 389
HpyF10VI GCNNNNNNNGC 3 cut(s) 187, 204, 374
HpyF3I CTNAG 4 cut(s) 36, 162, 209, 321
Hsp92I GRCGYC 1 cut(s) 130
Hsp92II CATG 1 cut(s) 508
Kzo9I GATC 4 cut(s) 459, 463, 501, 516
LmnI GCTCC 1 cut(s) 203
Lsp1109I GCAGC 2 cut(s) 97, 220
LweI GCATC 3 cut(s) 265, 269, 298
MalI GATC 4 cut(s) 461, 465, 503, 518
MboI GATC 4 cut(s) 459, 463, 501, 516
MboII GAAGA 4 cut(s) 77, 135, 448, 465
MflI RGATCY 1 cut(s) 516
MhlI GDGCHC 1 cut(s) 345
MlsI TGGCCA 1 cut(s) 414
MluNI TGGCCA 1 cut(s) 414
MlyI GAGTC 2 cut(s) 173, 404
MnlI CCTC 5 cut(s) 75, 218, 282, 358, 401
Mox20I TGGCCA 1 cut(s) 414
MscI TGGCCA 1 cut(s) 414
MseI TTAA 1 cut(s) 483
MslI CAYNNNNRTG 1 cut(s) 338
Msp20I TGGCCA 1 cut(s) 414
MspI CCGG 1 cut(s) 499
MspR9I CCNGG 2 cut(s) 265, 527
MvaI CCWGG 2 cut(s) 265, 527
MvnI CGCG 1 cut(s) 135
MwoI GCNNNNNNNGC 3 cut(s) 187, 204, 374
NdeII GATC 4 cut(s) 459, 463, 501, 516
NlaIII CATG 1 cut(s) 508
NmeAIII GCCGAG 1 cut(s) 216
PagI TCATGA 1 cut(s) 504
PfeI GAWTC 1 cut(s) 251
PflMI CCANNNNNTGG 1 cut(s) 526
PfoI TCCNGGA 1 cut(s) 263
PkrI GCNGC 3 cut(s) 87, 134, 235
PleI GAGTC 2 cut(s) 172, 403
PpsI GAGTC 2 cut(s) 172, 403
Psp6I CCWGG 2 cut(s) 263, 525
PspGI CCWGG 2 cut(s) 263, 525
PstNI CAGNNNCTG 1 cut(s) 368
PsuI RGATCY 1 cut(s) 516
RseI CAYNNNNRTG 1 cut(s) 338
SaqAI TTAA 1 cut(s) 483
SatI GCNGC 3 cut(s) 86, 133, 234
Sau3AI GATC 4 cut(s) 459, 463, 501, 516
SchI GAGTC 2 cut(s) 173, 404
ScrFI CCNGG 2 cut(s) 265, 527
SduI GDGCHC 1 cut(s) 345
SetI ASST 5 cut(s) 12, 35, 200, 412, 489
SfaNI GCATC 3 cut(s) 265, 269, 298
SmiMI CAYNNNNRTG 1 cut(s) 338
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
SsiI CCGC 1 cut(s) 133
StyD4I CCNGG 2 cut(s) 263, 525
StyI CCWWGG 1 cut(s) 475
TaaI ACNGT 3 cut(s) 116, 282, 305
TaqI TCGA 1 cut(s) 462
TauI GCSGC 1 cut(s) 135
TfiI GAWTC 1 cut(s) 251
Tru1I TTAA 1 cut(s) 483
Tru9I TTAA 1 cut(s) 483
TscAI CASTG 4 cut(s) 121, 246, 310, 396
TseI GCWGC 2 cut(s) 85, 233
TspDTI ATGAA 2 cut(s) 285, 322
TspRI CASTG 4 cut(s) 121, 246, 310, 396
Van91I CCANNNNNTGG 1 cut(s) 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.