RLG00000022372

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003475
Physical Location & Seq
Reverse (-)
82925169 .. 82932428
7260 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022372

Sequence Viewer

Length: 4905 bp
ATGGCTCACGGAAAAGTCAGTCTCCCTCAAGATCTCCTCCCCTCGCCGATCTTCTCCGCCACCAAAGATGGAGCCTTGGAAGGAAATGCTGAGGGAAAGGCTTTAACGGGGTCACTTGATGAGTCAAAAGATCATTTAGCATCTGAGAGCAGCATACCTTTGTCACCACAATGGCTACATGCTAAATCAGTTGAGGCTAAGCCACTTGCAAGTGGAACATCAGGAGAAATCCATGCACCAGGGTCTTTGTCTCATGGTAACTCTATTGATTCCAATGCGAGAGACATTTGGCGTTCAGATGGTTCTCGGGACAGAAAAGATTGGAGGAGGAATGCACCTGATTTAGATGTTAACCGTCGCTGGCGTGAGGAGGAGAGAGAAACAGGCTTACTTGGTAGGAGGGATCGCAAAAAAGAAGATCGCCGTGTAGGAGTCACTTCAACTAGGGATATTACTGAGAATCGAGCAGAAGATCATCGAGCAGGTGGCCCTTCTACTAGGGATGTTAGTGAGAATAAAATTTTGTCTTCTGATCGCTGGCATGAATCCCGGAGAGACAACAAATGGTCATCAAGGTGGGGCCCTGAAGATAAAGACAAGGACTCTCGAATAGAGAAGACAGAACTTGAGAGAGAAGATGCTCATGTTGACAAGCAGTCTCTTGTCAGTAGCAATCGTGCAATTCCTGAACGTGATGGTGATTCTCGCGATAAATGGAGGCCGCGCCATCGCATGGAGGTTCAGTCCGGGGGGCCAGCCCCATACCGTGCCGCACCGGGATTTGGGATGGCCAGAGGACAGGTGGAAAAAGTAGGATTTGCTTCTGGACGAGGGAGGTCCAAGACTAATGGTAGCCTACAGATTGGAAAACCTTCCTCTGTATCCACTATCGGGTCTGTTCTGGATCAGAATCAAAGTGTATTGGGAAAAGCTGGCCCCTTTAATGAGAAGTATCGTTACCCCAGGGGAAAGCTCCTTGATATCTATCGTAAGCAAAAGATGGAATCAACTTTCGATATTGTGCCTGATGGGATGGAGCATGTCTCTCCAATTACCCAAGTGGGCTCTATTGAGCCATTGGCCTTTTCTGCACCTCATGCAGAGGAAGAGGCTGTTATGGAGGATATATGGAAGGGAAGAATCACTAGCAGTGAAATGTTATGCAGTTCCTCCAGAGAAAAAAATGTGTTAAATGATGATGGTAAAGGAACTAGTAATGCCTCCTTAAGAAAGGAAGATCAAAAGATTTCACTGCAGACTGAAGAAAATGCCCTATCTACTGGAGAGCCCATCTTAAACAATTCTTTTCCAGTTATCGGTACTGAGGTCTCAACTATCTGTGGCCCACAGACACATATTTTAAATGAATGGGTTGAAGGTGAGCACAGAGTTCCTATTGTCACAGCAGTCGCTGATGGATCAAATCTGGTTGCTTCAATGAACTATGATTGCAAAAGTGCTGGGGAGATTGATGGCCTCAGTAATAGTGTCACTGAACTAAGAATTTCTGGATGCCAGGATGTGAGAGATTTTGGTCTTTCAAAGCATTCTATGTCGGAAAATTTTGGACCAGCTACTACTGTGATTGGTAGCCAGCTTCCTGATGATTCAAGTTCACTTTTTCATATACCATCTCTACAGCAAACCTTGATTAGTGATCAATATGTCAAGAGCAATGAGAAGGCAGATCTGGAAAGGGTTGTCTCGTTTGAGGATTTAAGTTTATGCTATCTTGATCCTCAAGGAAACATTCAGGGACCTTTCCTGGGGATCGACATAATTTCATGGTTTGATCAAGGATTTTTTGGAACTGACTTACTGGTGCGCTTGGCTGATGCCCCTGATGGTTCTCCTTTTCAAGAACTCGGTGAAATGATGCCAAATCTGAACACTAAATCTGGATTGGTCTCCAACTCCAATTTGCCTACCAACATGGAATCATTTGGTGGTGTTGGAAGCATCCTGGAAGAGAGAAAAACTGCTCCTCACTATGAGGAGTCTAACATTCTAAATATCCAACACTGGACTTCATCTGGACTTGAACCTACCTTGAGTGATGATTTGCACTTGAGAATACCTAATAATGGTTACAACTCTGAACTCCAGTACCTTGATAATCAAAGGTTTCAAAATTATGTTGAAGAAGATGAGGAAATAGTGTTTCCCGGAAGGCCTAAAAGTAGCAGTGATTTTCCTCTGAGGAGACCTTCAGCTGATATTCAGGGCTCATTTTCAAATTCCCCAAGCCTTCCTTCCCTTTCAAATGAAGTTTCCGAAAGTAACCTGCCTAATCGCCAGGATGATAAGTTGCACCCTTTTGGCCTGTTGATGTCTGAGCTCAGAGGCAGCTCTCATTTAAGACCTGCACAAGCTTCCAATGTGTCTTTAGGCATGGATGATCAGGCTCAGTTTAGAGATACCTTGTTTGAGGGAGGTATCTCCAAACTGAATTGGTTGAATAATCCTAATATCCCAATGCATCAAGTAGTGCCTGATATGGAACATCTAATAGAACTACAGCGTCAAGAGCAGCAGCATCGCCTATTGGAGCTTCAGCAACAACGCCAGTTTGAGTTTCATCGTCAGCAGCAGCACGAGTTGGAATTTCAACAGCAGCAGCAGCGGCAATTGGAACTCCAGCAACAGCGCCAGTTGGAACTTCACCACTTGGAGCTTCAGCAGCAGCACCAGTTGGAGCTACAGCTGCAGCGCCAGTTGGAGCTACAGCAACAGCGCCAGTTTGAGCTACAGAAACAGCGCCAGTTGGAGCTACAGCAACAACGCCAGTTGGAGCTTCAGCAACAACAGCAACACCATTTGGAGCTTCAACAACAGCAGCAGCGGAAGCTGGAGCTTCAGCAGCTGCAGCGTCATCAGCAACAGCGGCAGTTGGAGTTTCAGCAGCAGCGTCAATTGGAGCTTCAGCAAGAGCAGCATCAATTGGAGCTTCCGCCTCAGCACCACTTGCAGCAGCACCTTCGCCATCTCAAAAAATTGCAGCAACAACAGCAGTCCCAAGCTGAACAATTGCTCCTCGAACAATTATTGAATCGACAAATGTCTGATGGTTATGGGCAGTGGAAGATGGATGCTACACGAGCCAACCTTCATGATCAGTTACATTCCAGTAACCGCCTTCCAAATGATTTGCACCATGTATCTCATTCATCAAGGCACGATCCCTCGCTAGAGCAGATCATCCAAGCAAGTATGGGTGAGGGTGCGCTCCAAGGACAAACTGATTTTTTTGACCTCATATCGCAGGCCAAGCAAGGGAATAGGAACACTTCAGAGCTGCCACTTTGTCTTAATGAACAAGAGTTGCAAGCACAGCAATTATCTTTGGCATTGAGAGAGCAGCAGCAGATAGAAGGGGAAAGGCGTATTGGTGGGCCTTGGTTTGCAGATGAAGCAGGTCCTTTTTTGAGGGATCCCGTTGGGCATCACCAGGCTCACATGTTGGGGCATAATTCTTCAGAAAATTACCAGCAACGGCAGAGGTTTCCATCCAATGAACAGGAACCAAGCCATCATGATTGGAGTCATGCCTCTCAGGAGAGACCTCAGGCAGGGTTTTATGAACCTAACTCTATGGAACTTGATACATTCTCTGTTCCTGCTGGTTCTCATGGAATGAACTTGGATATTGCAAACATACGTGGACAAGATCTAGATGTGAGGGAACAATATCGGCTTAATTCCTCTATTGATGAGCGTGGATCTCTATCATCTGGCGTACCCTCTCACCGTCGTTTGGTTTCTGATGAGTTTTATGCTTCTCATCCAGTTACTTTTGAAAGCTTGCCATCTGGGAACAATGGGCAGCTAGAAAGCAGCTGCAGTGAAGCAGTGATGCCTTATTTGCATCTTGATGCTGAGCAAAAACGACGAGACACGGAAGACACCATTGCTTTTGCTGCTTCAAATAGATTGGCTTCAACTAACGGTGATAAGGAACACTCCAAACAAATTTTGATGGACCTTCAACAAAAACTTGGTCATCAGCCTGCACAGTCATCAGAAGTTGATTATCAGCATCACCTATCCTCATCCAGAAGTCAGGGAGGCTCTGTACATCTTCCTTTAAATCTTCTTCCAGATCCGTCAGTTGGTATGAGCAACTGCTTCACAGAAGGCCCACAGAGTTCAAATTTTAGTCTCTTGTTGCAAGATCAGTTGGGTAGTTATGGTATGAATGAGCAACCAAGTAATCTGGCAAGAAGATCTGATTCCAGAGCATTTGTGGACGCTCAATTCTTGTCAGGAACCAGGGATGCTCCTCATGAAGGCAACATGGCAAACCAACAAGACACAGCCATTGAGAGTGGGGAACTACCTAGTAATACCCATAGTAGGCATAGCTCACTGAGCAGTGCTGGTGGATGTGGGGGTTTTCACAGCTCTGAGATGGGATTAGATAAATCACTTGGAGAAGATGTTTCTGATGGGAGGCTGCCTTCTACTATAACTAAAGGGTTTGACAGTGCTACACAGAGACGCCGCGTCTTATCTTCACAGGATGTCTCATCTGAGTCTGCACTCTCTCTGCCTGTCAAGCCAAGAAGCTCCATAAGCCTCAGAACTTCTGAAAAAAATCCTGCTGCCACACTGGTCTCTGAATCTCGGGCATCTAGGAAAAATGATGCACAGTTTAGGAGGACTTCATCTTACAGTGATGCTGGTGTCTCTGAGACATCCTTCATAGATGTGCTCAAGAAACCAGTCATTCCAGAGGCTGATGTAGCCAACAGGGGTGCAGTGGAGTCATCTGATGGAGGTGGCCAAGTGGGGCGAACTGGCAAGAAGAAGGGCAAGAAAGGAAGACAGATCGATCCTGCTCTCCTTGGCTTTAAGGTATCCAGCAACCGGATCATGATGGGTGAGATCCATCGCCTTGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1635

Amino Acids

183.17

Weight (kDa)

5.61

Isoelectric Point (pI)

61.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 577 - 621 1.5e-10 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 473
Acc36I ACCTGC 4 cut(s) 473, 2292, 2371, 3404
AccB7I CCANNNNNTGG 1 cut(s) 733
AccII CGCG 3 cut(s) 708, 724, 4503
AciI CCGC 9 cut(s) 57, 722, 771, 2623, 2842, 2884, 2951, 3133, 4501
AcoI YGGCCR 2 cut(s) 789, 4780
AcsI RAATTY 7 cut(s) 519, 1503, 1561, 2236, 2603, 3969, 4150
AcyI GRCGYC 1 cut(s) 4498
AfaI GTAC 4 cut(s) 1321, 2108, 3738, 4074
AflII CTTAAG 1 cut(s) 1225
AflIII ACRYGT 1 cut(s) 3456
AhdI GACNNNNNGTC 2 cut(s) 655, 4502
AhlI ACTAGT 1 cut(s) 1211
AjnI CCWGG 8 cut(s) 238, 962, 1515, 1764, 1962, 2295, 3447, 4268
AjuI GAANNNNNNNTTGG 4 cut(s) 2236, 2268, 3369, 3401
AloI GAACNNNNNNTCC 2 cut(s) 3015, 3047
Alw21I GWGCWC 3 cut(s) 1386, 2340, 4713
AlwNI CAGNNNCTG 3 cut(s) 1412, 2863, 4736
Ama87I CYCGRG 2 cut(s) 306, 4623
ApaI GGGCCC 1 cut(s) 584
ApoI RAATTY 7 cut(s) 519, 1503, 1561, 2236, 2603, 3969, 4150
ArsI GACNNNNNNTTYG 2 cut(s) 3982, 4014
Asp700I GAANNNNTTC 1 cut(s) 871
AspLEI GCGC 7 cut(s) 726, 1827, 2649, 2712, 2736, 2760, 3226
AsuC2I CCSGG 4 cut(s) 550, 748, 777, 2166
AvaI CYCGRG 2 cut(s) 306, 4623
AvaII GGWCC 5 cut(s) 837, 1568, 1757, 3416, 3979
AxyI CCTNAGG 1 cut(s) 3564
BaeGI GKGCMC 1 cut(s) 584
BaeI ACNNNNGTAYC 4 cut(s) 1311, 1344, 2090, 2123
BalI TGGCCA 2 cut(s) 791, 4782
BamHI GGATCC 1 cut(s) 3430
BanII GRGCYC 5 cut(s) 584, 1067, 1290, 2228, 2340
BarI GAAGNNNNNNTAC 2 cut(s) 941, 973
BauI CACGAG 2 cut(s) 2594, 3096
BbsI GAAGAC 4 cut(s) 519, 623, 3906, 4828
Bbv12I GWGCWC 3 cut(s) 1386, 2340, 4713
BbvCI CCTCAGC 2 cut(s) 90, 2955
BceAI ACGGC 2 cut(s) 408, 3509
BcgI CGANNNNNNTGC 4 cut(s) 2519, 2553, 2960, 2994
BciT130I CCWGG 8 cut(s) 240, 964, 1517, 1766, 1964, 2297, 3449, 4270
BciVI GTATCC 2 cut(s) 892, 4867
BclI TGATCA 4 cut(s) 1657, 1792, 2398, 3112
BcnI CCSGG 4 cut(s) 550, 748, 777, 2166
BcuI ACTAGT 1 cut(s) 1211
BfaI CTAG 9 cut(s) 444, 498, 1146, 1212, 3188, 3671, 3827, 4338, 4632
BfoI RGCGCY 4 cut(s) 2650, 2713, 2737, 2761
BfrI CTTAAG 1 cut(s) 1225
BfuAI ACCTGC 4 cut(s) 473, 2292, 2371, 3404
BfuI GTATCC 2 cut(s) 892, 4867
BglII AGATCT 4 cut(s) 31, 1687, 3667, 4223
BlpI GCTNAGC 2 cut(s) 198, 3876
Bme18I GGWCC 5 cut(s) 837, 1568, 1757, 3416, 3979
BmeRI GACNNNNNGTC 2 cut(s) 655, 4502
BmeT110I CYCGRG 2 cut(s) 306, 4623
BmiI GGNNCC 9 cut(s) 73, 581, 582, 753, 937, 1758, 3432, 3522, 4267
BoxI GACNNNNGTC 1 cut(s) 3058
BpiI GAAGAC 4 cut(s) 519, 623, 3906, 4828
BplI GAGNNNNNCTC 2 cut(s) 1028, 1060
BpmI CTGGAG 5 cut(s) 1156, 1302, 2087, 2621, 2870
Bpu10I CCTNAGC 2 cut(s) 90, 2955
Bpu1102I GCTNAGC 2 cut(s) 198, 3876
BpuEI CTTGAG 6 cut(s) 12, 647, 1725, 2071, 2089, 4697
BpuMI CCSGG 4 cut(s) 550, 748, 777, 2166
Bsa29I ATCGAT 1 cut(s) 4830
BsaAI YACGTR 1 cut(s) 3659
BsaBI GATNNNNATC 3 cut(s) 909, 984, 3724
BsaHI GRCGYC 1 cut(s) 4498
BsaI GGTCTC 5 cut(s) 1333, 1912, 2197, 3553, 4618
BsaWI WCCGGW 1 cut(s) 4866
BsaXI ACNNNNNCTCC 8 cut(s) 728, 758, 3015, 3045, 4056, 4086, 4422, 4452
Bse21I CCTNAGG 1 cut(s) 3564
Bse3DI GCAATG 2 cut(s) 1681, 3906
Bse8I GATNNNNATC 3 cut(s) 909, 984, 3724
BseBI CCWGG 8 cut(s) 240, 964, 1517, 1766, 1964, 2297, 3449, 4270
BseCI ATCGAT 1 cut(s) 4830
BseJI GATNNNNATC 3 cut(s) 909, 984, 3724
BseMI GCAATG 2 cut(s) 1681, 3906
BseRI GAGGAG 9 cut(s) 26, 340, 383, 386, 1974, 2009, 2215, 3023, 4269
BseSI GKGCMC 1 cut(s) 584
BseYI CCCAGC 1 cut(s) 1460
BsgI GTGCAG 5 cut(s) 1074, 2349, 3993, 4521, 4776
Bsh1236I CGCG 3 cut(s) 708, 724, 4503
BshVI ATCGAT 1 cut(s) 4830
BsiHKAI GWGCWC 3 cut(s) 1386, 2340, 4713
BsiHKCI CYCGRG 2 cut(s) 306, 4623
BsiSI CCGG 5 cut(s) 550, 747, 776, 2166, 4867
BslFI GGGAC 3 cut(s) 323, 1770, 2998
BsmBI CGTCTC 1 cut(s) 4489
BsmFI GGGAC 3 cut(s) 323, 1770, 2998
BsmI GAATGC 2 cut(s) 337, 1546
Bso31I GGTCTC 5 cut(s) 1333, 1912, 2197, 3553, 4618
BsoBI CYCGRG 2 cut(s) 306, 4623
Bsp120I GGGCCC 1 cut(s) 580
Bsp1286I GDGCHC 7 cut(s) 584, 1067, 1290, 1386, 2228, 2340, 4713
Bsp1407I TGTACA 1 cut(s) 4072
Bsp1720I GCTNAGC 2 cut(s) 198, 3876
Bsp68I TCGCGA 1 cut(s) 708
BspACI CCGC 9 cut(s) 57, 722, 771, 2623, 2842, 2884, 2951, 3133, 4501
BspDI ATCGAT 1 cut(s) 4830
BspFNI CGCG 3 cut(s) 708, 724, 4503
BspHI TCATGA 4 cut(s) 3109, 3532, 4282, 4872
BspLI GGNNCC 9 cut(s) 73, 581, 582, 753, 937, 1758, 3432, 3522, 4267
BspMAI CTGCAG 4 cut(s) 1257, 2709, 2868, 3842
BspMI ACCTGC 4 cut(s) 473, 2292, 2371, 3404
BspTI CTTAAG 1 cut(s) 1225
BspTNI GGTCTC 5 cut(s) 1333, 1912, 2197, 3553, 4618
BsrDI GCAATG 2 cut(s) 1681, 3906
BsrGI TGTACA 1 cut(s) 4072
BssNI GRCGYC 1 cut(s) 4498
BssSI CACGAG 2 cut(s) 2594, 3096
BssT1I CCWWGG 4 cut(s) 75, 3229, 3395, 4843
Bst2BI CACGAG 2 cut(s) 2594, 3096
Bst2UI CCWGG 8 cut(s) 240, 964, 1517, 1766, 1964, 2297, 3449, 4270
Bst4CI ACNGT 9 cut(s) 356, 767, 1582, 3749, 3947, 4014, 4484, 4650, 4673
Bst6I CTCTTC 2 cut(s) 1101, 1962
BstACI GRCGYC 1 cut(s) 4498
BstAFI CTTAAG 1 cut(s) 1225
BstAPI GCANNNNNTGC 2 cut(s) 1097, 2965
BstAUI TGTACA 1 cut(s) 4072
BstBAI YACGTR 1 cut(s) 3659
BstC8I GCNNGC 9 cut(s) 362, 539, 756, 934, 1595, 3264, 3327, 3803, 4008
BstENI CCTNNNNNAGG 2 cut(s) 3567, 4284
BstFNI CGCG 3 cut(s) 708, 724, 4503
BstH2I RGCGCY 4 cut(s) 2650, 2713, 2737, 2761
BstHHI GCGC 7 cut(s) 726, 1827, 2649, 2712, 2736, 2760, 3226
BstNI CCWGG 8 cut(s) 240, 964, 1517, 1766, 1964, 2297, 3449, 4270
BstNSI RCATGY 3 cut(s) 182, 1043, 3460
BstPAI GACNNNNGTC 1 cut(s) 3058
BstSLI GKGCMC 1 cut(s) 584
BstUI CGCG 3 cut(s) 708, 724, 4503
BstV2I GAAGAC 4 cut(s) 519, 623, 3906, 4828
BstX2I RGATCY 8 cut(s) 31, 1687, 3430, 3667, 3719, 4099, 4223, 4884
BstYI RGATCY 8 cut(s) 31, 1687, 3430, 3667, 3719, 4099, 4223, 4884
Bsu15I ATCGAT 1 cut(s) 4830
Bsu36I CCTNAGG 1 cut(s) 3564
BsuI GTATCC 2 cut(s) 892, 4867
BsuTUI ATCGAT 1 cut(s) 4830
BtgZI GCGATG 3 cut(s) 713, 2522, 4874
BtsI GCAGTG 8 cut(s) 1156, 1250, 2191, 3083, 3847, 3855, 4378, 4764
BtuMI TCGCGA 1 cut(s) 708
BveI ACCTGC 4 cut(s) 473, 2292, 2371, 3404
Cac8I GCNNGC 9 cut(s) 362, 539, 756, 934, 1595, 3264, 3327, 3803, 4008
CaiI CAGNNNCTG 3 cut(s) 1412, 2863, 4736
CciI TCATGA 4 cut(s) 3109, 3532, 4282, 4872
CfoI GCGC 7 cut(s) 726, 1827, 2649, 2712, 2736, 2760, 3226
ClaI ATCGAT 1 cut(s) 4830
CseI GACGC 6 cut(s) 2510, 2858, 2897, 4256, 4492, 4506
Csp6I GTAC 4 cut(s) 1320, 2107, 3737, 4073
CviQI GTAC 4 cut(s) 1320, 2107, 3737, 4073
DraI TTTAAA 2 cut(s) 1362, 4086
DriI GACNNNNNGTC 2 cut(s) 655, 4502
EaeI YGGCCR 2 cut(s) 789, 4780
Eam1104I CTCTTC 2 cut(s) 1101, 1962
Eam1105I GACNNNNNGTC 2 cut(s) 655, 4502
EarI CTCTTC 2 cut(s) 1101, 1962
EciI GGCGGA 2 cut(s) 46, 2940
Ecl136II GAGCTC 1 cut(s) 2338
Eco130I CCWWGG 4 cut(s) 75, 3229, 3395, 4843
Eco147I AGGCCT 1 cut(s) 2173
Eco24I GRGCYC 5 cut(s) 584, 1067, 1290, 2228, 2340
Eco31I GGTCTC 5 cut(s) 1333, 1912, 2197, 3553, 4618
Eco32I GATATC 1 cut(s) 982
Eco47I GGWCC 5 cut(s) 837, 1568, 1757, 3416, 3979
Eco53kI GAGCTC 1 cut(s) 2338
Eco81I CCTNAGG 1 cut(s) 3564
Eco88I CYCGRG 2 cut(s) 306, 4623
EcoICRI GAGCTC 1 cut(s) 2338
EcoNI CCTNNNNNAGG 2 cut(s) 3567, 4284
EcoO109I RGGNCCY 4 cut(s) 580, 581, 1757, 3416
EcoRII CCWGG 8 cut(s) 238, 962, 1515, 1764, 1962, 2295, 3447, 4268
EcoRV GATATC 1 cut(s) 982
EcoT14I CCWWGG 4 cut(s) 75, 3229, 3395, 4843
EcoT22I ATGCAT 1 cut(s) 2481
EcoT38I GRGCYC 5 cut(s) 584, 1067, 1290, 2228, 2340
ErhI CCWWGG 4 cut(s) 75, 3229, 3395, 4843
Esp3I CGTCTC 1 cut(s) 4489
FalI AAGNNNNNCTT 2 cut(s) 2236, 2268
FaqI GGGAC 3 cut(s) 323, 1770, 2998
FbaI TGATCA 4 cut(s) 1657, 1792, 2398, 3112
FriOI GRGCYC 5 cut(s) 584, 1067, 1290, 2228, 2340
FspBI CTAG 9 cut(s) 444, 498, 1146, 1212, 3188, 3671, 3827, 4338, 4632
GlaI GCGC 7 cut(s) 725, 1826, 2648, 2711, 2735, 2759, 3225
GsaI CCCAGC 1 cut(s) 1464
GsuI CTGGAG 5 cut(s) 1156, 1302, 2087, 2621, 2870
HaeII RGCGCY 4 cut(s) 2650, 2713, 2737, 2761
HapII CCGG 5 cut(s) 550, 747, 776, 2166, 4867
HgaI GACGC 6 cut(s) 2510, 2858, 2897, 4256, 4492, 4506
HhaI GCGC 7 cut(s) 726, 1827, 2649, 2712, 2736, 2760, 3226
Hin1I GRCGYC 1 cut(s) 4498
Hin6I GCGC 7 cut(s) 724, 1825, 2647, 2710, 2734, 2758, 3224
HinP1I GCGC 7 cut(s) 724, 1825, 2647, 2710, 2734, 2758, 3224
HincII GTYRAC 2 cut(s) 352, 649
HindII GTYRAC 2 cut(s) 352, 649
HindIII AAGCTT 2 cut(s) 2370, 3799
HpaI GTTAAC 1 cut(s) 352
HpaII CCGG 5 cut(s) 550, 747, 776, 2166, 4867
Hpy166II GTNNAC 5 cut(s) 352, 649, 1616, 3662, 4246
Hpy8I GTNNAC 5 cut(s) 352, 649, 1616, 3662, 4246
Hpy99I CGWCG 3 cut(s) 360, 3753, 3891
HpyCH4III ACNGT 9 cut(s) 356, 767, 1582, 3749, 3947, 4014, 4484, 4650, 4673
HpyCH4IV ACGT 2 cut(s) 691, 3658
HpySE526I ACGT 2 cut(s) 691, 3658
Hsp92I GRCGYC 1 cut(s) 4498
HspAI GCGC 7 cut(s) 724, 1825, 2647, 2710, 2734, 2758, 3224
Ksp22I TGATCA 4 cut(s) 1657, 1792, 2398, 3112
KspAI GTTAAC 1 cut(s) 352
MaeI CTAG 9 cut(s) 444, 498, 1146, 1212, 3188, 3671, 3827, 4338, 4632
MaeII ACGT 2 cut(s) 691, 3658
MfeI CAATTG 4 cut(s) 2627, 2912, 2939, 3026
MflI RGATCY 8 cut(s) 31, 1687, 3430, 3667, 3719, 4099, 4223, 4884
MhlI GDGCHC 7 cut(s) 584, 1067, 1290, 1386, 2228, 2340, 4713
MlsI TGGCCA 2 cut(s) 791, 4782
MluNI TGGCCA 2 cut(s) 791, 4782
MlyI GAGTC 7 cut(s) 131, 441, 596, 2006, 3550, 4541, 4772
Mox20I TGGCCA 2 cut(s) 791, 4782
Mph1103I ATGCAT 1 cut(s) 2481
MroXI GAANNNNTTC 1 cut(s) 871
MscI TGGCCA 2 cut(s) 791, 4782
MslI CAYNNNNRTG 4 cut(s) 169, 574, 3870, 4706
Msp20I TGGCCA 2 cut(s) 791, 4782
MspA1I CMGCKG 7 cut(s) 2213, 2623, 2704, 2842, 2863, 2884, 3837
MspCI CTTAAG 1 cut(s) 1225
MspI CCGG 5 cut(s) 550, 747, 776, 2166, 4867
MunI CAATTG 4 cut(s) 2627, 2912, 2939, 3026
Mva1269I GAATGC 2 cut(s) 337, 1546
MvaI CCWGG 8 cut(s) 240, 964, 1517, 1766, 1964, 2297, 3449, 4270
MvnI CGCG 3 cut(s) 708, 724, 4503
NciI CCSGG 4 cut(s) 550, 748, 777, 2166
NlaIV GGNNCC 9 cut(s) 73, 581, 582, 753, 937, 1758, 3432, 3522, 4267
NmuCI GTSAC 5 cut(s) 111, 162, 433, 1399, 1489
NruI TCGCGA 1 cut(s) 708
NsiI ATGCAT 1 cut(s) 2481
NspI RCATGY 3 cut(s) 182, 1043, 3460
PagI TCATGA 4 cut(s) 3109, 3532, 4282, 4872
PaqCI CACCTGC 1 cut(s) 473
PasI CCCWGGG 1 cut(s) 963
PceI AGGCCT 1 cut(s) 2173
PciI ACATGT 1 cut(s) 3456
PctI GAATGC 2 cut(s) 337, 1546
PdmI GAANNNNTTC 1 cut(s) 871
PflMI CCANNNNNTGG 1 cut(s) 733
PfoI TCCNGGA 3 cut(s) 548, 1962, 2164
PleI GAGTC 7 cut(s) 130, 440, 596, 2005, 3549, 4540, 4771
PpsI GAGTC 7 cut(s) 130, 440, 596, 2005, 3549, 4540, 4771
Ppu21I YACGTR 1 cut(s) 3659
PpuMI RGGWCCY 2 cut(s) 1757, 3416
PscI ACATGT 1 cut(s) 3456
PshAI GACNNNNGTC 1 cut(s) 3058
Psp124BI GAGCTC 1 cut(s) 2340
Psp5II RGGWCCY 2 cut(s) 1757, 3416
Psp6I CCWGG 8 cut(s) 238, 962, 1515, 1764, 1962, 2295, 3447, 4268
PspFI CCCAGC 1 cut(s) 1460
PspGI CCWGG 8 cut(s) 238, 962, 1515, 1764, 1962, 2295, 3447, 4268
PspN4I GGNNCC 9 cut(s) 73, 581, 582, 753, 937, 1758, 3432, 3522, 4267
PspOMI GGGCCC 1 cut(s) 580
PspPPI RGGWCCY 2 cut(s) 1757, 3416
PstI CTGCAG 4 cut(s) 1257, 2709, 2868, 3842
PstNI CAGNNNCTG 3 cut(s) 1412, 2863, 4736
PsuI RGATCY 8 cut(s) 31, 1687, 3430, 3667, 3719, 4099, 4223, 4884
PvuII CAGCTG 4 cut(s) 2213, 2704, 2863, 3837
RruI TCGCGA 1 cut(s) 708
RsaI GTAC 4 cut(s) 1321, 2108, 3738, 4074
RsaNI GTAC 4 cut(s) 1320, 2107, 3737, 4073
RseI CAYNNNNRTG 4 cut(s) 169, 574, 3870, 4706
SacI GAGCTC 1 cut(s) 2340
SchI GAGTC 7 cut(s) 131, 441, 596, 2006, 3550, 4541, 4772
SduI GDGCHC 7 cut(s) 584, 1067, 1290, 1386, 2228, 2340, 4713
SinI GGWCC 5 cut(s) 837, 1568, 1757, 3416, 3979
SmiMI CAYNNNNRTG 4 cut(s) 169, 574, 3870, 4706
SmlI CTYRAG 7 cut(s) 27, 626, 1225, 1740, 2050, 2068, 4712
SmoI CTYRAG 7 cut(s) 27, 626, 1225, 1740, 2050, 2068, 4712
SpeI ACTAGT 1 cut(s) 1211
SseBI AGGCCT 1 cut(s) 2173
SsiI CCGC 9 cut(s) 57, 722, 771, 2623, 2842, 2884, 2951, 3133, 4501
SspMI CTAG 9 cut(s) 444, 498, 1146, 1212, 3188, 3671, 3827, 4338, 4632
SstI GAGCTC 1 cut(s) 2340
StuI AGGCCT 1 cut(s) 2173
StyI CCWWGG 4 cut(s) 75, 3229, 3395, 4843
TaaI ACNGT 9 cut(s) 356, 767, 1582, 3749, 3947, 4014, 4484, 4650, 4673
TaiI ACGT 2 cut(s) 694, 3661
TaqI TCGA 8 cut(s) 463, 478, 607, 1014, 1773, 3036, 3052, 4830
TatI WGTACW 1 cut(s) 4072
TauI GCSGC 5 cut(s) 724, 773, 2626, 2887, 4503
TseFI GTSAC 5 cut(s) 111, 162, 433, 1399, 1489
Tsp45I GTSAC 5 cut(s) 111, 162, 433, 1399, 1489
TspGWI ACGGA 3 cut(s) 24, 3911, 4092
Van91I CCANNNNNTGG 1 cut(s) 733
Vha464I CTTAAG 1 cut(s) 1225
VpaK11BI GGWCC 5 cut(s) 837, 1568, 1757, 3416, 3979
XagI CCTNNNNNAGG 2 cut(s) 3567, 4284
XapI RAATTY 7 cut(s) 519, 1503, 1561, 2236, 2603, 3969, 4150
XbaI TCTAGA 1 cut(s) 3670
XceI RCATGY 3 cut(s) 182, 1043, 3460
XcmI CCANNNNNNNNNTGG 2 cut(s) 799, 4240
XmnI GAANNNNTTC 1 cut(s) 871
XspI CTAG 9 cut(s) 444, 498, 1146, 1212, 3188, 3671, 3827, 4338, 4632
Zsp2I ATGCAT 1 cut(s) 2481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.