Rroxscaffold_2G00076870

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003475
Physical Location & Seq
Forward (+)
569105 .. 574656
5552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00076870.1

Sequence Viewer

Length: 4173 bp
ATGCTAGATGGAGCCTTGGAAGGAAATGCTGAGGGAAAGGCTCTAACGGGGTCACTTGATGAGTCAAAAGATCATTTAGCATCTGAGAGCAGCATACCTTTGTCACCACAATGGCTACATGCTAAATCAGTTGAGGCTAAGCCACTTGCAAGTGGAACATCAGGAGAAATCCATGCACCAGGGTCTTTGTCTCATGGTAACTCTATTGATTCCAATGCGAGAGACATTTGGCGTTCAGATGGTTCTCGGGACAGAAAAGATTGGAGGAGGAATGCACCTGATTTAGATGTTAACCGTCGCTGGCGTGAGGAGGAGAGAGAAACAGGCTTACTTGGTAGGAGGGATCGCAAAAAAGAAGATCGCCGTGTAGGAGTCACTTCAACTAGGGATATTACTGAGAATCGAGCAGAAGATCATCGAGCAGGTGGCCCTTCTACTAGGGATGTTAGTGAGAATAAAATTTTGTCTTCTGATCGCTGGCATGAATCCCGGAGAGACAACAAATGGTCATCAAGGTGGGGCCCTGAAGATAAAGACAAGGACTCTCGAATAGAGAAGACAGAACTTGAGAGAGAAGATGCTCATGTTGACAAGCAGTCTCTTGTCAGTAGCAATCGTGCAATTCCTGAACGTGATGGTGATTCTCGCGATAAATGGAGGCCACGCCATCGCATGGAGGTTCAGTCGGGGGGCCCAGCCCCATACCGTGCTGCACCGGGATTTGGGATGGCCAGAGGACAGGTGGAAAAAGTAGGATTTGCTTCTGGACGAGGGAGGTCCAAGACTAATGGTAGCCTACAGATTGGAAAACCTTCCTCTGTATCCACTATCGGGTCTGTTCTGGATCAGAATCAAAGTGTATTGGGAAAAGTTGGCCCCTTTAATGAGAAGTATCGTTACCCCAGGGGAAAGCTCCTTGATATCTATCGTAAGCAAAAGATAGAATCAACTTTCGATATTGTGCCTGATGGGATGGAGCATGTCTCTCCAATTACCCAAGTGGGCTCTATTGAGCCATTGGCCTTTTCTGCACCTCATGCAGAGGAAGAGGCTGTTATGGAAGATATATGGAAGGGAAGAATCACTAGCAGTGAAATGTTATGCAGTTCCTCCAGAGAAAAAAATGTGTTAAATGATGATGGTAAAGGAACTAGTAATGCCTCCTTAAGAAAGGAAGATCAAAAGATTTCACTGCAGACTGAAGAAAATGCCCTATCGACTGGAGAGCCCATCTTAAACAATTCTTTTCCAGTTATCAGTACTGAGGTCTCAACTATCTGCGGCCCACAGACACATATTTTAAATGAATGGGTTGAAGGTGAGCGCAGAGTTCGTATTACCACAGCAGTCGCTGATGGATCAAATCTGGTTGCTTCAAAGAACTATGATTGCAAAAGTGCTGGGGAGATTGATGGCCTCAGTAATAGTGTCGCTGAACTAAGAATTTCTGGATGCCAGGATGTGAGAGATTTTGGTCTTCCAAAGCATTCTATGTCGGAAAATTTTGAACCAGCTACTACTGTGATTGGTAGCCAGCTTCCTGATGATTCAAGTTCACTTTTTCATATTCCATCTCTACAGCAAACCTCGAGTAGTGATCAATATGTCAAGAGCAATGAGAAGGAAGATCTGGAAAGGGTTGTCTCATTTGAGGATTTAAGTTTATGTTATCTTGATCCTCAAGGAAACATCCAGGGACCTTTCCTGGGGATCGATATAATTTCATGGTTTGATCAAGGATTCTTTGGAACTGACTTACTGGTGCGCTTGGCTGATGCCCCTGATGGTTCTCCTTTTCAAGAACTTGGTGAAATGATGCCAAATCTGAACACTAAATCTGGATTTGTCTCCAACTCCAATTTGCCTACCAACATGGAATCATTTGGTGGTGTTGGAAGCATCCTGGAAGAGAGAAAACTGCTCCTCACTATGAGGAAAATAGTGTTTCCCGGAAGGCCTAAAAGTAGCAGTGATTTTCCTCTGAGGAGACCTTCAGCTGATATTCAGGGCTCATTTTCAAATTCCCCAAGCCTTCCTTCCCTTTCAAATGAAGTTTCCGAAAGTAACCTGCCTAATCGCCAGGATGATAAGTTGCACCCTTTTGGCCTGTTGATGTCTGAGCTCAGAGGCAGCTCTCATTTAAGACCTGCTCAAGCTTCCAATGTGTCTTTAGGCATGGATGATCAGGCTCAGTTTAGAGATACCTTGTTTGAGGGAGGTATCTCCAAACTGAATTGGTTGAATAATCCTAATATCCCAATGCATCAAGTAGTGCCTGATATGGAACATCTAATAGAACTACAGCGTCAAGAGCAGCAGCATCGCCTATTGGAGCTTCAGCAACAACGCCAGTTTGAGTTTCATCGTCAGCAGCAGCACGAGTTGGAATTTCAACAGCAGCAGCAGCGGCAATTGGAACTCCAGCAACAGCGCCAGTTGGAACTTCACCACTTGGAGCTTCAGCAGCAGCACCAGTTGGAGCTACAGCTGCAGCGCCAGTTGGAGCTACAGCAACAGCGCCAGTTTGAGCTACAGCAACAGCACCAGTTGGAGCTACAGAAACAGCGCCAGTTGGAGCTTCAGCAACAGCAGCAACACCGATTGGAGCTTCAACAACAGCAGCAGCGGAAGCTGGAGCTTCAGCAGCTGCAGCGTCATCAGCAACAGCGGCAGTTGGAGTTTCAGCAGCAGCGTCAACTGGAGCTTCAGCAACAGCAGCATCAATTGGAGCTTCCGCCTCAGCACCACTTGCAGCAGCACCTTCGCCATCACCAAAAATTGCAGCAACAACAGCAGTCCCAAGCCGAACAATTGCTCCTCGAACAATTATTGAATCGGCAAATGTCTGATGGTTATGGGCAGTGGAAGATGGATGCTACACGAGCCAACCTTCGTGATCAGTTACATTCCAGTAACCACCTTCCAAATGATTTGCACCATGTATCTCATTCATCAAGGCACGATCCCTCGCTAGAGCAGATCATCCAAGCAACTATGGGCGAGGGTGCGCTCCAAGGACAGACTGATTTTTTTGACCTCATATCGCAGGCCAAGCAAGGGAATAGGAACACTTCAGAGCTGCCACTTCGTCTTAATGAACAAGAGTTGCAAGCACAGCAATTATCTTTGGCATTGAGAGAGCAGCAGCAGATAGAAGGGGAAAGGCGTATTGGTGGGCCTTGGTTTGCAGATGAAGCAGGTCCTTTTTTGAGGGATCCCGTTGGCCATCACCAGGCTCACATGTTGGGGCATAATTCTTCAGAAAATTACCAGCAACAGCAGAGTTTTCCATCCAATGAACAGGAACCAAGCCATCATGATTGGAGTCATGCCTCTCAGGAGATACCTCAGGGAGGGTTTTATGAACCCAACTCTATGGAATTTGATAGATTCTCTGTTCCTGCTGGTTCTCATGGAATGAACTTGGATATTGCAAACATACGTGGACAAGATCTAGATGTGAGGGAACAATATCGGCTTAATTCCTCTATTGATGAGCGTGGATCTCTATCATCTGGCGTACCCTCCCACCGTCGTTTGGTTTCTGATGAGTTTTATGCTTCTCATCCAGTTACTTTTGAAAGCTTGCCATCTGGGAACAATGGGCAGCTAGAAAGTAGCTGCACTGAAACAGTAATGCCTTATTTGCATCTTGATGCTGAGCAAAAACGACGAGACACGGAAGACACCATTGCCTATGCTGCTTCAAATAGATTGGCTTCAACTAACGGTGAGAAGGAACACTCCAAACAAATTTTGATGGACCTTCATCAAAAACTTGGTCATCAGCCTTCACAGTCATCAGAAGTTGATTATCAGCATCACCTATCCTCATCCAGAAGTCAGGGAGGCTCTGTACATCTTCCTTTAAATCTTCTTCCAGTTCAGTCAGTTGGTATGAGCAACTGCTTCACAGAAGGCCCACAGAGTTCAAATTTTAGTCTCTTGTTGCAAGATCAGTTGGGTAGTCATGGTATGAATGAGCAAACAAGTAATCTGGCAAGAAGATCTGATTCCAGAGCATTTATGGACGCTCAATTCTTGTCAGGAACCAGGGATGCTCCTCATGAAGTCAACATGGCAAACCAACAAGACACAGCCATTGAGAGCGGGGAACTACCTAGTAATACCCATAGCAGGCATAGCTCACTGAGCAGTGCTGGTAATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1390

Amino Acids

157.07

Weight (kDa)

5.65

Isoelectric Point (pI)

64.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 557 - 601 1.2e-10 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 413
Acc36I ACCTGC 4 cut(s) 413, 2076, 2155, 3188
AccB7I CCANNNNNTGG 1 cut(s) 673
AccBSI CCGCTC 1 cut(s) 4110
AccII CGCG 1 cut(s) 648
AciI CCGC 6 cut(s) 1281, 2407, 2626, 2668, 2735, 4110
AclWI GGATC 9 cut(s) 351, 852, 1366, 1670, 1718, 2957, 3209, 3222, 3511
AcoI YGGCCR 2 cut(s) 729, 3223
AcsI RAATTY 8 cut(s) 459, 1443, 1501, 2020, 2387, 3380, 3753, 3934
AfaI GTAC 3 cut(s) 1261, 3522, 3858
AfiI CCNNNNNNNGG 9 cut(s) 673, 722, 831, 1706, 3057, 3232, 3353, 3538, 4137
AflII CTTAAG 1 cut(s) 1165
AflIII ACRYGT 1 cut(s) 3240
AhdI GACNNNNNGTC 1 cut(s) 595
AhlI ACTAGT 1 cut(s) 1151
AjnI CCWGG 9 cut(s) 178, 902, 1455, 1692, 1704, 1902, 2079, 3231, 4052
AjuI GAANNNNNNNTTGG 4 cut(s) 2020, 2052, 3153, 3185
AloI GAACNNNNNNTCC 2 cut(s) 2799, 2831
Alw21I GWGCWC 1 cut(s) 2124
AlwI GGATC 9 cut(s) 351, 852, 1366, 1670, 1718, 2957, 3209, 3222, 3511
AlwNI CAGNNNCTG 2 cut(s) 1352, 2647
Ama87I CYCGRG 2 cut(s) 246, 1588
ApaI GGGCCC 2 cut(s) 524, 695
ApoI RAATTY 8 cut(s) 459, 1443, 1501, 2020, 2387, 3380, 3753, 3934
ArsI GACNNNNNNTTYG 2 cut(s) 3766, 3798
Asp700I GAANNNNTTC 1 cut(s) 811
AspLEI GCGC 7 cut(s) 1326, 1767, 2433, 2496, 2520, 2568, 3010
AsuC2I CCSGG 3 cut(s) 490, 717, 1950
AsuHPI GGTGA 9 cut(s) 96, 650, 1331, 1820, 2438, 2762, 3221, 3743, 3815
AvaI CYCGRG 2 cut(s) 246, 1588
AvaII GGWCC 4 cut(s) 777, 1697, 3200, 3763
AxyI CCTNAGG 1 cut(s) 3348
BaeGI GKGCMC 2 cut(s) 524, 695
BalI TGGCCA 2 cut(s) 731, 3225
BamHI GGATCC 1 cut(s) 3214
BanII GRGCYC 6 cut(s) 524, 695, 1007, 1230, 2012, 2124
BarI GAAGNNNNNNTAC 2 cut(s) 881, 913
BauI CACGAG 2 cut(s) 2378, 2880
BbsI GAAGAC 4 cut(s) 459, 563, 1469, 3690
Bbv12I GWGCWC 1 cut(s) 2124
BbvCI CCTCAGC 2 cut(s) 30, 2739
BceAI ACGGC 1 cut(s) 348
BcgI CGANNNNNNTGC 4 cut(s) 2303, 2337, 2744, 2778
BciT130I CCWGG 9 cut(s) 180, 904, 1457, 1694, 1706, 1904, 2081, 3233, 4054
BciVI GTATCC 1 cut(s) 832
BclI TGATCA 4 cut(s) 1597, 1732, 2182, 2896
BcnI CCSGG 3 cut(s) 490, 717, 1950
BcuI ACTAGT 1 cut(s) 1151
BfaI CTAG 9 cut(s) 5, 384, 438, 1086, 1152, 2972, 3455, 3611, 4122
BfoI RGCGCY 4 cut(s) 2434, 2497, 2521, 2569
BfrI CTTAAG 1 cut(s) 1165
BfuAI ACCTGC 4 cut(s) 413, 2076, 2155, 3188
BfuI GTATCC 1 cut(s) 832
BglII AGATCT 3 cut(s) 1627, 3451, 4007
BlpI GCTNAGC 2 cut(s) 138, 3660
BmcAI AGTACT 1 cut(s) 1261
Bme18I GGWCC 4 cut(s) 777, 1697, 3200, 3763
BmeRI GACNNNNNGTC 1 cut(s) 595
BmeT110I CYCGRG 2 cut(s) 246, 1588
BpiI GAAGAC 4 cut(s) 459, 563, 1469, 3690
BplI GAGNNNNNCTC 2 cut(s) 968, 1000
BpmI CTGGAG 5 cut(s) 1096, 1242, 2405, 2654, 2720
Bpu10I CCTNAGC 2 cut(s) 30, 2739
Bpu1102I GCTNAGC 2 cut(s) 138, 3660
BpuEI CTTGAG 3 cut(s) 587, 1665, 2136
BpuMI CCSGG 3 cut(s) 490, 717, 1950
Bsa29I ATCGAT 1 cut(s) 1713
BsaAI YACGTR 1 cut(s) 3443
BsaBI GATNNNNATC 3 cut(s) 849, 924, 3508
BsaI GGTCTC 2 cut(s) 1273, 1981
BsaJI CCNNGG 9 cut(s) 15, 179, 902, 903, 1693, 1705, 3013, 3179, 4053
BsaXI ACNNNNNCTCC 6 cut(s) 668, 698, 2799, 2829, 3840, 3870
Bsc4I CCNNNNNNNGG 9 cut(s) 673, 722, 831, 1706, 3057, 3232, 3353, 3538, 4137
Bse21I CCTNAGG 1 cut(s) 3348
Bse3DI GCAATG 2 cut(s) 1621, 3690
Bse8I GATNNNNATC 3 cut(s) 849, 924, 3508
BseBI CCWGG 9 cut(s) 180, 904, 1457, 1694, 1706, 1904, 2081, 3233, 4054
BseCI ATCGAT 1 cut(s) 1713
BseDI CCNNGG 9 cut(s) 15, 179, 902, 903, 1693, 1705, 3013, 3179, 4053
BseJI GATNNNNATC 3 cut(s) 849, 924, 3508
BseLI CCNNNNNNNGG 9 cut(s) 673, 722, 831, 1706, 3057, 3232, 3353, 3538, 4137
BseMI GCAATG 2 cut(s) 1621, 3690
BseRI GAGGAG 7 cut(s) 280, 323, 326, 1913, 1999, 2807, 4053
BseSI GKGCMC 2 cut(s) 524, 695
BseYI CCCAGC 2 cut(s) 694, 1400
BsgI GTGCAG 3 cut(s) 696, 1014, 3607
Bsh1236I CGCG 1 cut(s) 648
BshVI ATCGAT 1 cut(s) 1713
BsiHKAI GWGCWC 1 cut(s) 2124
BsiHKCI CYCGRG 2 cut(s) 246, 1588
BsiSI CCGG 3 cut(s) 490, 716, 1950
BslFI GGGAC 3 cut(s) 263, 1710, 2782
BslI CCNNNNNNNGG 9 cut(s) 673, 722, 831, 1706, 3057, 3232, 3353, 3538, 4137
BsmFI GGGAC 3 cut(s) 263, 1710, 2782
BsmI GAATGC 2 cut(s) 277, 1486
Bso31I GGTCTC 2 cut(s) 1273, 1981
BsoBI CYCGRG 2 cut(s) 246, 1588
Bsp120I GGGCCC 2 cut(s) 520, 691
Bsp1286I GDGCHC 6 cut(s) 524, 695, 1007, 1230, 2012, 2124
Bsp1407I TGTACA 1 cut(s) 3856
Bsp1720I GCTNAGC 2 cut(s) 138, 3660
Bsp68I TCGCGA 1 cut(s) 648
BspACI CCGC 6 cut(s) 1281, 2407, 2626, 2668, 2735, 4110
BspDI ATCGAT 1 cut(s) 1713
BspFNI CGCG 1 cut(s) 648
BspHI TCATGA 2 cut(s) 3316, 4066
BspMAI CTGCAG 3 cut(s) 1197, 2493, 2652
BspMI ACCTGC 4 cut(s) 413, 2076, 2155, 3188
BspPI GGATC 9 cut(s) 351, 852, 1366, 1670, 1718, 2957, 3209, 3222, 3511
BspTI CTTAAG 1 cut(s) 1165
BspTNI GGTCTC 2 cut(s) 1273, 1981
BsrBI CCGCTC 1 cut(s) 4110
BsrDI GCAATG 2 cut(s) 1621, 3690
BsrGI TGTACA 1 cut(s) 3856
BssECI CCNNGG 9 cut(s) 15, 179, 902, 903, 1693, 1705, 3013, 3179, 4053
BssSI CACGAG 2 cut(s) 2378, 2880
BssT1I CCWWGG 3 cut(s) 15, 3013, 3179
Bst2BI CACGAG 2 cut(s) 2378, 2880
Bst2UI CCWGG 9 cut(s) 180, 904, 1457, 1694, 1706, 1904, 2081, 3233, 4054
Bst4CI ACNGT 7 cut(s) 296, 707, 1522, 3533, 3634, 3731, 3798
Bst6I CTCTTC 2 cut(s) 1041, 1902
BstAFI CTTAAG 1 cut(s) 1165
BstAPI GCANNNNNTGC 2 cut(s) 1037, 2749
BstAUI TGTACA 1 cut(s) 3856
BstBAI YACGTR 1 cut(s) 3443
BstC8I GCNNGC 7 cut(s) 302, 479, 1535, 3048, 3111, 3587, 4139
BstENI CCTNNNNNAGG 1 cut(s) 3351
BstFNI CGCG 1 cut(s) 648
BstH2I RGCGCY 4 cut(s) 2434, 2497, 2521, 2569
BstHHI GCGC 7 cut(s) 1326, 1767, 2433, 2496, 2520, 2568, 3010
BstNI CCWGG 9 cut(s) 180, 904, 1457, 1694, 1706, 1904, 2081, 3233, 4054
BstNSI RCATGY 3 cut(s) 122, 983, 3244
BstSLI GKGCMC 2 cut(s) 524, 695
BstUI CGCG 1 cut(s) 648
BstV2I GAAGAC 4 cut(s) 459, 563, 1469, 3690
BstX2I RGATCY 5 cut(s) 1627, 3214, 3451, 3503, 4007
BstXI CCANNNNNNTGG 1 cut(s) 3376
BstYI RGATCY 5 cut(s) 1627, 3214, 3451, 3503, 4007
Bsu15I ATCGAT 1 cut(s) 1713
Bsu36I CCTNAGG 1 cut(s) 3348
BsuI GTATCC 1 cut(s) 832
BsuTUI ATCGAT 1 cut(s) 1713
BtgZI GCGATG 2 cut(s) 653, 2306
BtsI GCAGTG 5 cut(s) 1096, 1190, 1975, 2867, 4162
BtsIMutI CAGTG 7 cut(s) 1096, 1190, 1975, 2867, 3624, 4148, 4162
BtuMI TCGCGA 1 cut(s) 648
BveI ACCTGC 4 cut(s) 413, 2076, 2155, 3188
Cac8I GCNNGC 7 cut(s) 302, 479, 1535, 3048, 3111, 3587, 4139
CaiI CAGNNNCTG 2 cut(s) 1352, 2647
CciI TCATGA 2 cut(s) 3316, 4066
CfoI GCGC 7 cut(s) 1326, 1767, 2433, 2496, 2520, 2568, 3010
ClaI ATCGAT 1 cut(s) 1713
CseI GACGC 4 cut(s) 2294, 2642, 2681, 4040
Csp6I GTAC 3 cut(s) 1260, 3521, 3857
CviQI GTAC 3 cut(s) 1260, 3521, 3857
DraI TTTAAA 2 cut(s) 1302, 3870
DriI GACNNNNNGTC 1 cut(s) 595
EaeI YGGCCR 2 cut(s) 729, 3223
Eam1104I CTCTTC 2 cut(s) 1041, 1902
Eam1105I GACNNNNNGTC 1 cut(s) 595
EarI CTCTTC 2 cut(s) 1041, 1902
EciI GGCGGA 1 cut(s) 2724
Ecl136II GAGCTC 1 cut(s) 2122
Eco130I CCWWGG 3 cut(s) 15, 3013, 3179
Eco147I AGGCCT 1 cut(s) 1957
Eco24I GRGCYC 6 cut(s) 524, 695, 1007, 1230, 2012, 2124
Eco31I GGTCTC 2 cut(s) 1273, 1981
Eco32I GATATC 1 cut(s) 922
Eco47I GGWCC 4 cut(s) 777, 1697, 3200, 3763
Eco53kI GAGCTC 1 cut(s) 2122
Eco81I CCTNAGG 1 cut(s) 3348
Eco88I CYCGRG 2 cut(s) 246, 1588
EcoICRI GAGCTC 1 cut(s) 2122
EcoNI CCTNNNNNAGG 1 cut(s) 3351
EcoO109I RGGNCCY 5 cut(s) 520, 521, 691, 1697, 3200
EcoRII CCWGG 9 cut(s) 178, 902, 1455, 1692, 1704, 1902, 2079, 3231, 4052
EcoRV GATATC 1 cut(s) 922
EcoT14I CCWWGG 3 cut(s) 15, 3013, 3179
EcoT22I ATGCAT 1 cut(s) 2265
EcoT38I GRGCYC 6 cut(s) 524, 695, 1007, 1230, 2012, 2124
ErhI CCWWGG 3 cut(s) 15, 3013, 3179
FalI AAGNNNNNCTT 2 cut(s) 2020, 2052
FaqI GGGAC 3 cut(s) 263, 1710, 2782
FauI CCCGC 1 cut(s) 4103
FbaI TGATCA 4 cut(s) 1597, 1732, 2182, 2896
FriOI GRGCYC 6 cut(s) 524, 695, 1007, 1230, 2012, 2124
FspBI CTAG 9 cut(s) 5, 384, 438, 1086, 1152, 2972, 3455, 3611, 4122
GlaI GCGC 7 cut(s) 1325, 1766, 2432, 2495, 2519, 2567, 3009
GsaI CCCAGC 2 cut(s) 698, 1404
GsuI CTGGAG 5 cut(s) 1096, 1242, 2405, 2654, 2720
HaeII RGCGCY 4 cut(s) 2434, 2497, 2521, 2569
HapII CCGG 3 cut(s) 490, 716, 1950
HgaI GACGC 4 cut(s) 2294, 2642, 2681, 4040
HhaI GCGC 7 cut(s) 1326, 1767, 2433, 2496, 2520, 2568, 3010
Hin6I GCGC 7 cut(s) 1324, 1765, 2431, 2494, 2518, 2566, 3008
HinP1I GCGC 7 cut(s) 1324, 1765, 2431, 2494, 2518, 2566, 3008
HincII GTYRAC 4 cut(s) 292, 589, 2696, 4075
HindII GTYRAC 4 cut(s) 292, 589, 2696, 4075
HindIII AAGCTT 2 cut(s) 2154, 3583
HpaI GTTAAC 1 cut(s) 292
HpaII CCGG 3 cut(s) 490, 716, 1950
HphI GGTGA 9 cut(s) 96, 650, 1331, 1820, 2438, 2762, 3221, 3743, 3815
Hpy166II GTNNAC 6 cut(s) 292, 589, 1556, 2696, 3446, 4075
Hpy8I GTNNAC 6 cut(s) 292, 589, 1556, 2696, 3446, 4075
Hpy99I CGWCG 3 cut(s) 300, 3537, 3675
HpyCH4III ACNGT 7 cut(s) 296, 707, 1522, 3533, 3634, 3731, 3798
HpyCH4IV ACGT 2 cut(s) 631, 3442
HpySE526I ACGT 2 cut(s) 631, 3442
HspAI GCGC 7 cut(s) 1324, 1765, 2431, 2494, 2518, 2566, 3008
Ksp22I TGATCA 4 cut(s) 1597, 1732, 2182, 2896
KspAI GTTAAC 1 cut(s) 292
MaeI CTAG 9 cut(s) 5, 384, 438, 1086, 1152, 2972, 3455, 3611, 4122
MaeII ACGT 2 cut(s) 631, 3442
MaeIII GTNAC 9 cut(s) 51, 102, 197, 373, 896, 2063, 2901, 2912, 3571
MbiI CCGCTC 1 cut(s) 4110
MfeI CAATTG 3 cut(s) 2411, 2723, 2810
MflI RGATCY 5 cut(s) 1627, 3214, 3451, 3503, 4007
MhlI GDGCHC 6 cut(s) 524, 695, 1007, 1230, 2012, 2124
MlsI TGGCCA 2 cut(s) 731, 3225
MluNI TGGCCA 2 cut(s) 731, 3225
MlyI GAGTC 4 cut(s) 71, 381, 536, 3334
Mox20I TGGCCA 2 cut(s) 731, 3225
Mph1103I ATGCAT 1 cut(s) 2265
MroXI GAANNNNTTC 1 cut(s) 811
MscI TGGCCA 2 cut(s) 731, 3225
MslI CAYNNNNRTG 3 cut(s) 109, 514, 3654
Msp20I TGGCCA 2 cut(s) 731, 3225
MspA1I CMGCKG 6 cut(s) 1997, 2407, 2488, 2626, 2647, 2668
MspCI CTTAAG 1 cut(s) 1165
MspI CCGG 3 cut(s) 490, 716, 1950
MunI CAATTG 3 cut(s) 2411, 2723, 2810
Mva1269I GAATGC 2 cut(s) 277, 1486
MvaI CCWGG 9 cut(s) 180, 904, 1457, 1694, 1706, 1904, 2081, 3233, 4054
MvnI CGCG 1 cut(s) 648
NciI CCSGG 3 cut(s) 490, 717, 1950
NmuCI GTSAC 3 cut(s) 51, 102, 373
NruI TCGCGA 1 cut(s) 648
NsiI ATGCAT 1 cut(s) 2265
NspI RCATGY 3 cut(s) 122, 983, 3244
PaeR7I CTCGAG 1 cut(s) 1588
PagI TCATGA 2 cut(s) 3316, 4066
PaqCI CACCTGC 1 cut(s) 413
PasI CCCWGGG 1 cut(s) 903
PceI AGGCCT 1 cut(s) 1957
PciI ACATGT 1 cut(s) 3240
PctI GAATGC 2 cut(s) 277, 1486
PdmI GAANNNNTTC 1 cut(s) 811
PflMI CCANNNNNTGG 1 cut(s) 673
PfoI TCCNGGA 3 cut(s) 488, 1902, 1948
PleI GAGTC 4 cut(s) 70, 380, 536, 3333
PpsI GAGTC 4 cut(s) 70, 380, 536, 3333
Ppu21I YACGTR 1 cut(s) 3443
PpuMI RGGWCCY 2 cut(s) 1697, 3200
PscI ACATGT 1 cut(s) 3240
Psp124BI GAGCTC 1 cut(s) 2124
Psp5II RGGWCCY 2 cut(s) 1697, 3200
Psp6I CCWGG 9 cut(s) 178, 902, 1455, 1692, 1704, 1902, 2079, 3231, 4052
PspFI CCCAGC 2 cut(s) 694, 1400
PspGI CCWGG 9 cut(s) 178, 902, 1455, 1692, 1704, 1902, 2079, 3231, 4052
PspOMI GGGCCC 2 cut(s) 520, 691
PspPPI RGGWCCY 2 cut(s) 1697, 3200
PspXI VCTCGAGB 1 cut(s) 1588
PstI CTGCAG 3 cut(s) 1197, 2493, 2652
PstNI CAGNNNCTG 2 cut(s) 1352, 2647
PsuI RGATCY 5 cut(s) 1627, 3214, 3451, 3503, 4007
PvuII CAGCTG 3 cut(s) 1997, 2488, 2647
RruI TCGCGA 1 cut(s) 648
RsaI GTAC 3 cut(s) 1261, 3522, 3858
RsaNI GTAC 3 cut(s) 1260, 3521, 3857
RseI CAYNNNNRTG 3 cut(s) 109, 514, 3654
SacI GAGCTC 1 cut(s) 2124
ScaI AGTACT 1 cut(s) 1261
SchI GAGTC 4 cut(s) 71, 381, 536, 3334
SduI GDGCHC 6 cut(s) 524, 695, 1007, 1230, 2012, 2124
Sfr274I CTCGAG 1 cut(s) 1588
SinI GGWCC 4 cut(s) 777, 1697, 3200, 3763
SlaI CTCGAG 1 cut(s) 1588
SmiMI CAYNNNNRTG 3 cut(s) 109, 514, 3654
SmlI CTYRAG 5 cut(s) 566, 1165, 1588, 1680, 2151
SmoI CTYRAG 5 cut(s) 566, 1165, 1588, 1680, 2151
SpeI ACTAGT 1 cut(s) 1151
SseBI AGGCCT 1 cut(s) 1957
SsiI CCGC 6 cut(s) 1281, 2407, 2626, 2668, 2735, 4110
SspMI CTAG 9 cut(s) 5, 384, 438, 1086, 1152, 2972, 3455, 3611, 4122
SstI GAGCTC 1 cut(s) 2124
StuI AGGCCT 1 cut(s) 1957
StyI CCWWGG 3 cut(s) 15, 3013, 3179
TaaI ACNGT 7 cut(s) 296, 707, 1522, 3533, 3634, 3731, 3798
TaiI ACGT 2 cut(s) 634, 3445
TaqI TCGA 8 cut(s) 403, 418, 547, 954, 1217, 1589, 1713, 2820
TatI WGTACW 2 cut(s) 1259, 3856
TauI GCSGC 3 cut(s) 1284, 2410, 2671
TscAI CASTG 7 cut(s) 1096, 1197, 1975, 2867, 3631, 4155, 4162
TseFI GTSAC 3 cut(s) 51, 102, 373
Tsp45I GTSAC 3 cut(s) 51, 102, 373
TspGWI ACGGA 1 cut(s) 3695
TspRI CASTG 7 cut(s) 1096, 1197, 1975, 2867, 3631, 4155, 4162
Van91I CCANNNNNTGG 1 cut(s) 673
Vha464I CTTAAG 1 cut(s) 1165
VpaK11BI GGWCC 4 cut(s) 777, 1697, 3200, 3763
XagI CCTNNNNNAGG 1 cut(s) 3351
XapI RAATTY 8 cut(s) 459, 1443, 1501, 2020, 2387, 3380, 3753, 3934
XbaI TCTAGA 1 cut(s) 3454
XceI RCATGY 3 cut(s) 122, 983, 3244
XcmI CCANNNNNNNNNTGG 2 cut(s) 739, 4024
XhoI CTCGAG 1 cut(s) 1588
XmnI GAANNNNTTC 1 cut(s) 811
XspI CTAG 9 cut(s) 5, 384, 438, 1086, 1152, 2972, 3455, 3611, 4122
ZrmI AGTACT 1 cut(s) 1261
Zsp2I ATGCAT 1 cut(s) 2265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.