MD09G1007900.v1.1

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003475
Physical Location & Seq
Forward (+)
549095 .. 556311
7217 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1007900.v1.1.491

Sequence Viewer

Length: 4485 bp
ATGGCGCATGGAAAAGTCAGTCTCCCTCAAGATCTCCTCCCCTCGAATCTGGTCGATTCACACTTCTCCGCCAAAGATGAAGCCTTCCAAGGAAACAGTGAGGAGAAGGCGCTAACGGGGTCCCTTGACTTGTCAATTGGTGATCAAGTTGCCTCGGACAGCAGCATCCCTTTGTCTCCACAATGGCTATATGCTAAACCAGATTCCAAGACACTAGCAACTGGAACGTCAGGAGAAGTGCATGCAAATGATTCTCTGTCTCATGGAAACTCAACTGATCCCAATTCGAGAGACATTTGGCGTGTAGATGGATCTCAGGAGAAAAAAGATTGGAAGAGGAATGCACCTGATTTAGATAGTAGTCGCCGCTGGCGTGAAGAGGAGAGAGAAACCGGCTTACTTGGTAGGAGAGATCGAAAGAAAGAAGATCGCCGTCTAGGTACTAGTTTGACCAGGGATAATACTGAGACTAGAGCAGAAGATCGCCGTGTAGGTGCGACTACAACTAAGGATGCTGCAGAGAATAAAGTATTGTCTTCTGACCGCTGGATTGAATCCCGAAGAGATAACAAATGGTCATCAAGGTGGGGGCCTGAAGATAAAGAGAAGGACTCACGAATAGAGAAAAAGACTGATGTTGAGAGGGAAGATGTACATGTCGAGAAGCAGTCTTTTTCCAACAGCAACCGTTCCGCTTCTGAGCGTGATAGTGATTCTCGTGATAAGTGGAGGCCGCGCCATCGCATAGACGGTCAATCAGCTGGGGCAGGCCCATATCGTGCTGCACCAGGATTTGGGATGGCTCGAGGGCAGGTGGAAAAAGTAGGATTTGCTGCTGGACGAGGGAGGTCTAACACTAATGGAAGCCTACAGATTGGCAAACTTGTACTAGGAAAATCTAGCTGTTTTGCCAATATGTATTGCTATCCCAGAGGAAAGCTCCTTGATATTTATCGCAAGCAAAAGAAGGATTCGACTTTTGATATGCCTGATGGGATGGAGCATGTATCACCAATAACCCAAGTGGGCTCGATTGAGCCATTGGCTTTTGTTGCCCCTGATGCAGAGGAAGAGGCTGGTCTGGGTGACATATATAAGGGAAGAATTACTAGCAGTGAAGTGGTATACAACTCGTCTGGGGACATGAATGTATTAGATGAAGATGCTAAAGGATCTAGCAATGTCGGTGTAAGCAAGGAGGAAGGAAAAAATGTCCAATCTGCTGAAAAGGTCGATTTAAACAATTCTTTTCCGGTTACTGGTGCTGAGTCACCTATTTGTGGCTCACAAACACATATTTTAAAAGAAAGTGTTGCTACTGAAGGTGAGCAGAAAGTTCTGGCGGTCACAGCAGTGGGTGATAGGGAGATTGATCCCAGTAATGATGACGCTGACTTAAAAAGTTCAAGATGCCAGGAGCAAACCGCAAGCAGTGATCATCAGTATTTGAAGAGCAATGAGGACGCCCATCTCTTGGAAAGAATTGTCACACCTGAGGATATGAGTTTGTGCTACCTTGATCCTCAAGGGAATATCCAAGGGCCTTTCCTGGGGATCGATATAATTGCATGGTTTGAGCAAGGATTTTTCGGAATTGACTTGCCAGTTCGTTCATTTGATGCGCCCAATGGATCTCTTTTTCAAGAACTTGGTGAAGTGATGCCACATCTGAAAACTAAATCTGGGCCTGTCTATAATTGTAGTTTGCCTACTCAATTAGAGCCATTGGATGCTGTTGGAGGCAGCTTGGAAGAGAGAACATCTGCTCCTAACTATGATGGGGGGTCTGCAATTCTAAACAATCAAGGCATTCAGAATGTTGTAGCAGAAGATGAGGAAATCGTATTTCCCGAAAGGCCCAAAAGCAGCAGTGATTGCCTATTGAGGTATTCAGCTGACATTCATGGCACATTTGCAAATCCTCCCAGCCTTCATTCCCTTGCAAATGAAGTTTTAGAAACTAGCATGCCTGATCAGCAGGACAATAAGTTGCATCCTTTTGGCCTGTTGATGTCAGAGCTCAGGGGCAACTCGCGTTTAAGGCCTGCTCAATCATCTCATGCGTCTTTCCGCATTGATGATCAGGTTCATTTTAGAGATTCCTTCTTTGAGGAAGGTGCCACCGTTGCTAATCAGAATTCCCTTGCTGCCATGATTGATCAACCTTGCTTTGTGGAGATACAGCCTGATAATTATATAAAAAATAACACTAATGTTAGTCTGGGTTCTATAGATCCTCACCACTTATCTCGCATGGGACAACAAAACAGTGGCTTTGACCCGGCAGAGTATATAATGTCACAACAATTACTAAAAGAACGGTTTCAGCAGCTGAACCATCTTTCTCCACTTACTGTTGCACATAGTGCAGGAACAGGAGTGGACCGGTTCCCTGGATTTGGTTTTCCTGACAGCAAGAACCCTAATATCCAGCCGTCGTTCCATCATCCGGTGGCAGATATGGAACATATTTTTGAAATGCAGCATCAGTTGGAGCTTCGTCAACAGCAACACCAGTTGGAGCTTCAGCAACAGCAGCGTCAGTTGGAGCTTCAGCAACAGCAGCGTAAGTTGGAGCTTCAGCAACACCAGCGTCGGTTGGAGCTTCAGCAACACCAGCGTCAGTTGGAGCTTCAGCAACAGCATCGTCAGTTGGAGATTCAGGAACACCAGCGTCAGGTGGAGCTGCAGCACCAGCATCAGTTGGAGCTGCATCACCAACGTCAATTGGAGCTTCAGCAGCAACAGCGTCAGCTGGAGCTTCAGCAACAGCGTCACTTGCAGGAGCAGCTTCACCAACACCAAATGAAATTGCAGCAACAACAGAAATCTCAAGCTCAACATGTGCTCCTTGAACAGTTTTTGCATCAACAAATATCGGATTCTGGTTATGGGCAGTGGAAGGCGGATGCTACAAGAGGCAACCCTTTCGATCAATTACATTCAAGGAAGCACATTCTAGATGACTTGCACCAGATATCCCATTCCTCAAGACACGATCCATTGTTAGAGCATATCATCCAAGAAAATATAGGACGCTCAGGACAGACTGATTTCTTTGACCTCATATCTCAGGCCAAGCAGGGGAATATGCACATTTCGGATCTGCAAGCACAGCAGTTGTCCGCAGCATTGAGAGAGCAACTGCAGATGGAAGGGGAAAGGCATGTCGGTGGGCCTCGGTTCGTAGATGAAGCAAGCCAAGTTGTCAGGAATCCTGTTGGTCATCACCAGGCTCAGATGGTAGGATTTAGCTCTTCAGAAAATTACCAGCAACAGCAGAGGTTTCCCACCCATGAACAGCAACTAAGCCATCTCAATTGGAATCATGCCACACGGGAGCAACCTCAGGGAGGGTTCTACGAACCTAACTCTCTGGAATTTGAGAGGTCATCTGTTCCTGCTAGTTCCCATGCGATGAACTTGGATATTCTGAGTGCACGCGGGCGGGGTCTGGATGAGTTTTATTCTTCTCATCCAGTTGGTTTGGAGCACTTGCCCTTTGGGAACAATGGACATCTGGAAGATAGCTCCATTGAATCACTGCGTCGTCATGCTGAGCAAACCCGCGACTCAGAAGCTACCATGGCTTTTGCTGATTCAGATATATGGGCTTCATCCGATGGTGATAAAGAACACTCAAAACAATTTTTGATGGACCTTCACCAAAAATTCAGTCAATCTATACAGTTATCAGAAGTTGATTATCAGCATCACCTATCGTCCTCCGGAAGCGGGGGAGAATCTGTACATTATCCATTTAATCTTCTTCCTGATCAACCAGTTGGTATGAACAACTCCTTCACAGAAGGGCCTTTTAGATCTAATTCCAGAGCATTTGTGGAAGATTTATCAGAGTTGGAGGGAAATAATTGGAAGAAACAGGGAGTGAAAAGCTATGAAGGCAACATGGGAGAACAAGAAGAAACATCAAGGGATCTTCGTAGTAATGCCCATAGCAGGCAGAGTTCACTTAGCAGTGCTGGTGGAGGCGGGAGTTTTTACAGCTCTGAGACAGGGATAGATAAACCACTTGGAGAAGAGGTTTCTAATGGGAGACTGCCTTCTATTGTAACCAAAGGGTATGACAATGCTTTGAATAGACGCCGAGTTTTATCTTCCCAGGACGTTTTGTCTGAAGCAGCACTTCCTTTACCTGTCAAACAAAGAAATCCAGCTGCAACCCAGGTCTCTGAAACTCAAGCATGTAGCAGGAATGATGCGCAATTTAGGAGGACTTCGTCTTACAGTGACACATCTGTGTCCGAGACATCATTCATAGACATGCTCAAGAAACCAGTCATTTCAGAGGCTGATGCGGCCAACAGAGCAGCTGCACTGGAATCGTCTGATGGAGGTGGCCAAGCGGGGCGCACTGGCAAGAAGAAAGGGAAGAAAGGAAGACAGATCGATCCTGCTCTACTCGGCTTTAAGGTTACCAGCAACCGGATCTTGATGGGTGAGATCCATCGCCTTAACGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1495

Amino Acids

167.27

Weight (kDa)

5.61

Isoelectric Point (pI)

57.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 505 - 538 2.6e-09 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 802
Acc16I TGCGCA 1 cut(s) 4224
Acc36I ACCTGC 1 cut(s) 802
AccB1I GGYRCC 1 cut(s) 2117
AccB7I CCANNNNNTGG 2 cut(s) 794, 1474
AccI GTMKAC 1 cut(s) 1125
AccII CGCG 4 cut(s) 736, 2035, 3444, 3570
AccIII TCCGGA 1 cut(s) 3728
AcoI YGGCCR 2 cut(s) 4320, 4360
AcsI RAATTY 3 cut(s) 2137, 3380, 3671
AcyI GRCGYC 2 cut(s) 1464, 4105
AdeI CACNNNGTG 1 cut(s) 2366
AfaI GTAC 4 cut(s) 442, 654, 888, 3750
AflIII ACRYGT 2 cut(s) 655, 2842
AgeI ACCGGT 1 cut(s) 2385
AgsI TTSAA 9 cut(s) 554, 1407, 1450, 1643, 2477, 2855, 2946, 3539, 4099
AhdI GACNNNNNGTC 1 cut(s) 4132
AhlI ACTAGT 1 cut(s) 443
AjnI CCWGG 8 cut(s) 452, 787, 1413, 1548, 2392, 3231, 4122, 4185
AleI CACNNNNGTG 2 cut(s) 1352, 4259
AloI GAACNNNNNNTCC 2 cut(s) 1750, 1782
Alw21I GWGCWC 4 cut(s) 2022, 2850, 3442, 3495
Alw26I GTCTC 9 cut(s) 26, 180, 264, 285, 461, 4007, 4051, 4195, 4262
Alw44I GTGCAC 1 cut(s) 3438
AlwNI CAGNNNCTG 2 cut(s) 2332, 4313
Ama87I CYCGRG 1 cut(s) 804
Aor13HI TCCGGA 1 cut(s) 3728
ApaLI GTGCAC 1 cut(s) 3438
ApoI RAATTY 3 cut(s) 2137, 3380, 3671
ArsI GACNNNNNNTTYG 2 cut(s) 3661, 3693
AsiGI ACCGGT 1 cut(s) 2385
Asp700I GAANNNNTTC 1 cut(s) 2322
AspLEI GCGC 6 cut(s) 7, 112, 738, 1624, 4225, 4374
AsuC2I CCSGG 1 cut(s) 2282
AvaI CYCGRG 1 cut(s) 804
AvaII GGWCC 3 cut(s) 120, 2383, 3658
AxyI CCTNAGG 2 cut(s) 1494, 3348
BaeGI GKGCMC 1 cut(s) 3442
BalI TGGCCA 1 cut(s) 4362
BanI GGYRCC 1 cut(s) 2117
BanII GRGCYC 2 cut(s) 1031, 2022
BauI CACGAG 1 cut(s) 717
BbsI GAAGAC 2 cut(s) 528, 4408
Bbv12I GWGCWC 4 cut(s) 2022, 2850, 3442, 3495
BceAI ACGGC 3 cut(s) 417, 471, 2419
BcgI CGANNNNNNTGC 8 cut(s) 1547, 1581, 2627, 2661, 2911, 2945, 4326, 4360
BciT130I CCWGG 8 cut(s) 454, 789, 1415, 1550, 2394, 3233, 4124, 4187
BclI TGATCA 6 cut(s) 142, 1435, 1972, 2080, 2158, 3775
BcnI CCSGG 1 cut(s) 2282
BcoDI GTCTC 9 cut(s) 26, 180, 264, 285, 461, 4007, 4051, 4195, 4262
BcuI ACTAGT 1 cut(s) 443
BfmI CTRYAG 5 cut(s) 516, 869, 2229, 2687, 3146
BfoI RGCGCY 1 cut(s) 113
BfuAI ACCTGC 1 cut(s) 802
BglII AGATCT 2 cut(s) 31, 3821
BlpI GCTNAGC 1 cut(s) 3558
Bme1390I CCNGG 9 cut(s) 454, 789, 1415, 1550, 2282, 2394, 3233, 4124, 4187
Bme18I GGWCC 3 cut(s) 120, 2383, 3658
BmeRI GACNNNNNGTC 1 cut(s) 4132
BmeT110I CYCGRG 1 cut(s) 804
BmiI GGNNCC 5 cut(s) 121, 122, 591, 2119, 2390
BmrFI CCNGG 9 cut(s) 454, 789, 1415, 1550, 2282, 2394, 3233, 4124, 4187
BmrI ACTGGG 1 cut(s) 1371
BmuI ACTGGG 1 cut(s) 1371
BpiI GAAGAC 2 cut(s) 528, 4408
BplI GAGNNNNNCTC 4 cut(s) 596, 628, 924, 956
BpmI CTGGAG 1 cut(s) 2777
Bpu10I CCTNAGC 2 cut(s) 2021, 3040
Bpu1102I GCTNAGC 1 cut(s) 3558
BpuEI CTTGAG 6 cut(s) 12, 1509, 2817, 2974, 4185, 4274
BpuMI CCSGG 1 cut(s) 2282
Bsa29I ATCGAT 2 cut(s) 1557, 4410
BsaBI GATNNNNATC 1 cut(s) 951
BsaHI GRCGYC 2 cut(s) 1464, 4105
BsaI GGTCTC 1 cut(s) 4195
BsaWI WCCGGW 5 cut(s) 1252, 2385, 2449, 3728, 4446
BsaXI ACNNNNNCTCC 8 cut(s) 1750, 1780, 2832, 2862, 3732, 3762, 4029, 4059
Bse118I RCCGGY 2 cut(s) 392, 2385
Bse21I CCTNAGG 2 cut(s) 1494, 3348
Bse3DI GCAATG 2 cut(s) 1186, 1462
Bse8I GATNNNNATC 1 cut(s) 951
BseAI TCCGGA 1 cut(s) 3728
BseBI CCWGG 8 cut(s) 454, 789, 1415, 1550, 2394, 3233, 4124, 4187
BseCI ATCGAT 2 cut(s) 1557, 4410
BseJI GATNNNNATC 1 cut(s) 951
BseMI GCAATG 2 cut(s) 1186, 1462
BseRI GAGGAG 3 cut(s) 26, 116, 395
BseSI GKGCMC 1 cut(s) 3442
BseYI CCCAGC 2 cut(s) 761, 1925
BsgI GTGCAG 3 cut(s) 768, 2388, 4320
Bsh1236I CGCG 4 cut(s) 736, 2035, 3444, 3570
BshNI GGYRCC 1 cut(s) 2117
BshTI ACCGGT 1 cut(s) 2385
BshVI ATCGAT 2 cut(s) 1557, 4410
BsiHKAI GWGCWC 4 cut(s) 2022, 2850, 3442, 3495
BsiHKCI CYCGRG 1 cut(s) 804
BsiSI CCGG 7 cut(s) 393, 1253, 2282, 2386, 2450, 3729, 4447
BslFI GGGAC 3 cut(s) 106, 1154, 2271
BsmAI GTCTC 9 cut(s) 26, 180, 264, 285, 461, 4007, 4051, 4195, 4262
BsmFI GGGAC 3 cut(s) 106, 1154, 2271
BsmI GAATGC 2 cut(s) 346, 1809
Bso31I GGTCTC 1 cut(s) 4195
BsoBI CYCGRG 1 cut(s) 804
Bsp1286I GDGCHC 5 cut(s) 1031, 2022, 2850, 3442, 3495
Bsp13I TCCGGA 1 cut(s) 3728
Bsp1407I TGTACA 2 cut(s) 652, 3748
Bsp1720I GCTNAGC 1 cut(s) 3558
Bsp19I CCATGG 1 cut(s) 3585
BspDI ATCGAT 2 cut(s) 1557, 4410
BspEI TCCGGA 1 cut(s) 3728
BspFNI CGCG 4 cut(s) 736, 2035, 3444, 3570
BspLI GGNNCC 5 cut(s) 121, 122, 591, 2119, 2390
BspMAI CTGCAG 3 cut(s) 520, 2691, 3150
BspMI ACCTGC 1 cut(s) 802
BspQI GCTCTTC 2 cut(s) 1445, 3262
BspT107I GGYRCC 1 cut(s) 2117
BspTNI GGTCTC 1 cut(s) 4195
BsrDI GCAATG 2 cut(s) 1186, 1462
BsrFI RCCGGY 2 cut(s) 392, 2385
BsrGI TGTACA 2 cut(s) 652, 3748
BssAI RCCGGY 2 cut(s) 392, 2385
BssNAI GTATAC 1 cut(s) 1126
BssNI GRCGYC 2 cut(s) 1464, 4105
BssSI CACGAG 1 cut(s) 717
BssT1I CCWWGG 3 cut(s) 88, 1537, 3585
Bst1107I GTATAC 1 cut(s) 1126
Bst2BI CACGAG 1 cut(s) 717
Bst2UI CCWGG 8 cut(s) 454, 789, 1415, 1550, 2394, 3233, 4124, 4187
Bst6I CTCTTC 8 cut(s) 329, 372, 556, 1065, 1445, 1746, 3262, 4035
BstACI GRCGYC 2 cut(s) 1464, 4105
BstAPI GCANNNNNTGC 2 cut(s) 1875, 2366
BstAUI TGTACA 2 cut(s) 652, 3748
BstDSI CCRYGG 1 cut(s) 3585
BstEII GGTNACC 1 cut(s) 4435
BstENI CCTNNNNNAGG 1 cut(s) 3351
BstFNI CGCG 4 cut(s) 736, 2035, 3444, 3570
BstH2I RGCGCY 1 cut(s) 113
BstHHI GCGC 6 cut(s) 7, 112, 738, 1624, 4225, 4374
BstMAI GTCTC 9 cut(s) 26, 180, 264, 285, 461, 4007, 4051, 4195, 4262
BstNI CCWGG 8 cut(s) 454, 789, 1415, 1550, 2394, 3233, 4124, 4187
BstNSI RCATGY 8 cut(s) 245, 659, 1007, 1969, 2846, 3170, 4209, 4288
BstPI GGTNACC 1 cut(s) 4435
BstSCI CCNGG 9 cut(s) 452, 787, 1413, 1548, 2280, 2392, 3231, 4122, 4185
BstSFI CTRYAG 5 cut(s) 516, 869, 2229, 2687, 3146
BstSLI GKGCMC 1 cut(s) 3442
BstUI CGCG 4 cut(s) 736, 2035, 3444, 3570
BstV2I GAAGAC 2 cut(s) 528, 4408
BstZ17I GTATAC 1 cut(s) 1126
Bsu15I ATCGAT 2 cut(s) 1557, 4410
Bsu36I CCTNAGG 2 cut(s) 1494, 3348
BsuTUI ATCGAT 2 cut(s) 1557, 4410
BtgI CCRYGG 1 cut(s) 3585
BtgZI GCGATG 3 cut(s) 725, 3431, 4454
BtsI GCAGTG 7 cut(s) 1120, 1359, 1438, 1876, 2903, 3542, 3985
BveI ACCTGC 1 cut(s) 802
CaiI CAGNNNCTG 2 cut(s) 2332, 4313
CfoI GCGC 6 cut(s) 7, 112, 738, 1624, 4225, 4374
Cfr10I RCCGGY 2 cut(s) 392, 2385
ClaI ATCGAT 2 cut(s) 1557, 4410
Csp6I GTAC 4 cut(s) 441, 653, 887, 3749
CspAI ACCGGT 1 cut(s) 2385
CviQI GTAC 4 cut(s) 441, 653, 887, 3749
DraI TTTAAA 2 cut(s) 1239, 1302
DraIII CACNNNGTG 1 cut(s) 2366
DriI GACNNNNNGTC 1 cut(s) 4132
EaeI YGGCCR 2 cut(s) 4320, 4360
Eam1104I CTCTTC 8 cut(s) 329, 372, 556, 1065, 1445, 1746, 3262, 4035
Eam1105I GACNNNNNGTC 1 cut(s) 4132
EarI CTCTTC 8 cut(s) 329, 372, 556, 1065, 1445, 1746, 3262, 4035
EciI GGCGGA 2 cut(s) 58, 2921
Ecl136II GAGCTC 1 cut(s) 2020
Eco130I CCWWGG 3 cut(s) 88, 1537, 3585
Eco147I AGGCCT 1 cut(s) 2044
Eco24I GRGCYC 2 cut(s) 1031, 2022
Eco31I GGTCTC 1 cut(s) 4195
Eco32I GATATC 1 cut(s) 2979
Eco47I GGWCC 3 cut(s) 120, 2383, 3658
Eco53kI GAGCTC 1 cut(s) 2020
Eco81I CCTNAGG 2 cut(s) 1494, 3348
Eco88I CYCGRG 1 cut(s) 804
Eco91I GGTNACC 1 cut(s) 4435
EcoICRI GAGCTC 1 cut(s) 2020
EcoNI CCTNNNNNAGG 1 cut(s) 3351
EcoO109I RGGNCCY 4 cut(s) 120, 590, 1541, 3812
EcoO65I GGTNACC 1 cut(s) 4435
EcoRI GAATTC 1 cut(s) 2137
EcoRII CCWGG 8 cut(s) 452, 787, 1413, 1548, 2392, 3231, 4122, 4185
EcoRV GATATC 1 cut(s) 2979
EcoT14I CCWWGG 3 cut(s) 88, 1537, 3585
EcoT38I GRGCYC 2 cut(s) 1031, 2022
ErhI CCWWGG 3 cut(s) 88, 1537, 3585
FalI AAGNNNNNCTT 2 cut(s) 4131, 4163
FaqI GGGAC 3 cut(s) 106, 1154, 2271
FauI CCCGC 6 cut(s) 3437, 3441, 3575, 3728, 3986, 4360
FbaI TGATCA 6 cut(s) 142, 1435, 1972, 2080, 2158, 3775
FblI GTMKAC 1 cut(s) 1125
FriOI GRGCYC 2 cut(s) 1031, 2022
FspI TGCGCA 1 cut(s) 4224
GlaI GCGC 6 cut(s) 6, 111, 737, 1623, 4224, 4373
GsaI CCCAGC 2 cut(s) 765, 1929
GsuI CTGGAG 1 cut(s) 2777
HaeII RGCGCY 1 cut(s) 113
HapII CCGG 7 cut(s) 393, 1253, 2282, 2386, 2450, 3729, 4447
HhaI GCGC 6 cut(s) 7, 112, 738, 1624, 4225, 4374
Hin1I GRCGYC 2 cut(s) 1464, 4105
Hin6I GCGC 6 cut(s) 5, 110, 736, 1622, 4223, 4372
HinP1I GCGC 6 cut(s) 5, 110, 736, 1622, 4223, 4372
HincII GTYRAC 1 cut(s) 2504
HindII GTYRAC 1 cut(s) 2504
HpaII CCGG 7 cut(s) 393, 1253, 2282, 2386, 2450, 3729, 4447
Hpy166II GTNNAC 5 cut(s) 1126, 2383, 2504, 3440, 3971
Hpy8I GTNNAC 5 cut(s) 1126, 2383, 2504, 3440, 3971
Hpy99I CGWCG 3 cut(s) 2440, 2598, 3552
HpyCH4IV ACGT 3 cut(s) 227, 2722, 4128
HpySE526I ACGT 3 cut(s) 227, 2722, 4128
Hsp92I GRCGYC 2 cut(s) 1464, 4105
HspAI GCGC 6 cut(s) 5, 110, 736, 1622, 4223, 4372
KflI GGGWCCC 1 cut(s) 120
Kpn2I TCCGGA 1 cut(s) 3728
Ksp22I TGATCA 6 cut(s) 142, 1435, 1972, 2080, 2158, 3775
LguI GCTCTTC 2 cut(s) 1445, 3262
MaeII ACGT 3 cut(s) 227, 2722, 4128
MfeI CAATTG 3 cut(s) 135, 2726, 3319
MhlI GDGCHC 5 cut(s) 1031, 2022, 2850, 3442, 3495
MlsI TGGCCA 1 cut(s) 4362
MluNI TGGCCA 1 cut(s) 4362
MlyI GAGTC 3 cut(s) 605, 1277, 3566
Mox20I TGGCCA 1 cut(s) 4362
MroI TCCGGA 1 cut(s) 3728
MroXI GAANNNNTTC 1 cut(s) 2322
MscI TGGCCA 1 cut(s) 4362
MseI TTAA 7 cut(s) 1238, 1301, 1397, 2039, 3762, 4431, 4476
MslI CAYNNNNRTG 6 cut(s) 246, 583, 1352, 3171, 4259, 4283
Msp20I TGGCCA 1 cut(s) 4362
MspA1I CMGCKG 8 cut(s) 369, 546, 761, 1895, 2332, 2755, 4178, 4334
MspI CCGG 7 cut(s) 393, 1253, 2282, 2386, 2450, 3729, 4447
MspR9I CCNGG 9 cut(s) 454, 789, 1415, 1550, 2282, 2394, 3233, 4124, 4187
MunI CAATTG 3 cut(s) 135, 2726, 3319
Mva1269I GAATGC 2 cut(s) 346, 1809
MvaI CCWGG 8 cut(s) 454, 789, 1415, 1550, 2394, 3233, 4124, 4187
MvnI CGCG 4 cut(s) 736, 2035, 3444, 3570
NciI CCSGG 1 cut(s) 2282
NcoI CCATGG 1 cut(s) 3585
NlaIV GGNNCC 5 cut(s) 121, 122, 591, 2119, 2390
NmeAIII GCCGAG 2 cut(s) 4133, 4404
NmuCI GTSAC 7 cut(s) 1085, 1269, 1345, 1486, 2298, 2774, 4250
NsbI TGCGCA 1 cut(s) 4224
NspI RCATGY 8 cut(s) 245, 659, 1007, 1969, 2846, 3170, 4209, 4288
OliI CACNNNNGTG 2 cut(s) 1352, 4259
PaeI GCATGC 2 cut(s) 245, 1969
PaeR7I CTCGAG 1 cut(s) 804
PaqCI CACCTGC 1 cut(s) 802
PceI AGGCCT 1 cut(s) 2044
PciI ACATGT 2 cut(s) 655, 2842
PciSI GCTCTTC 2 cut(s) 1445, 3262
PcsI WCGNNNNNNNCGW 2 cut(s) 370, 1848
PctI GAATGC 2 cut(s) 346, 1809
PdmI GAANNNNTTC 1 cut(s) 2322
PflFI GACNNNGTC 1 cut(s) 4240
PflMI CCANNNNNTGG 2 cut(s) 794, 1474
PinAI ACCGGT 1 cut(s) 2385
PleI GAGTC 3 cut(s) 605, 1276, 3566
PpsI GAGTC 3 cut(s) 605, 1276, 3566
PpuMI RGGWCCY 1 cut(s) 120
PscI ACATGT 2 cut(s) 655, 2842
Psp124BI GAGCTC 1 cut(s) 2022
Psp5II RGGWCCY 1 cut(s) 120
Psp6I CCWGG 8 cut(s) 452, 787, 1413, 1548, 2392, 3231, 4122, 4185
PspEI GGTNACC 1 cut(s) 4435
PspFI CCCAGC 2 cut(s) 761, 1925
PspGI CCWGG 8 cut(s) 452, 787, 1413, 1548, 2392, 3231, 4122, 4185
PspN4I GGNNCC 5 cut(s) 121, 122, 591, 2119, 2390
PspPPI RGGWCCY 1 cut(s) 120
PspXI VCTCGAGB 1 cut(s) 804
PstI CTGCAG 3 cut(s) 520, 2691, 3150
PstNI CAGNNNCTG 2 cut(s) 2332, 4313
PsyI GACNNNGTC 1 cut(s) 4240
PvuII CAGCTG 6 cut(s) 761, 1895, 2332, 2755, 4178, 4334
RsaI GTAC 4 cut(s) 442, 654, 888, 3750
RsaNI GTAC 4 cut(s) 441, 653, 887, 3749
RseI CAYNNNNRTG 6 cut(s) 246, 583, 1352, 3171, 4259, 4283
SacI GAGCTC 1 cut(s) 2022
SapI GCTCTTC 2 cut(s) 1445, 3262
SaqAI TTAA 7 cut(s) 1238, 1301, 1397, 2039, 3762, 4431, 4476
SchI GAGTC 3 cut(s) 605, 1277, 3566
ScrFI CCNGG 9 cut(s) 454, 789, 1415, 1550, 2282, 2394, 3233, 4124, 4187
SduI GDGCHC 5 cut(s) 1031, 2022, 2850, 3442, 3495
SfcI CTRYAG 5 cut(s) 516, 869, 2229, 2687, 3146
Sfr274I CTCGAG 1 cut(s) 804
SinI GGWCC 3 cut(s) 120, 2383, 3658
SlaI CTCGAG 1 cut(s) 804
SmiMI CAYNNNNRTG 6 cut(s) 246, 583, 1352, 3171, 4259, 4283
SmlI CTYRAG 7 cut(s) 27, 804, 1524, 2832, 2989, 4200, 4289
SmoI CTYRAG 7 cut(s) 27, 804, 1524, 2832, 2989, 4200, 4289
SpeI ACTAGT 1 cut(s) 443
SphI GCATGC 2 cut(s) 245, 1969
SseBI AGGCCT 1 cut(s) 2044
SstI GAGCTC 1 cut(s) 2022
StuI AGGCCT 1 cut(s) 2044
StyD4I CCNGG 9 cut(s) 452, 787, 1413, 1548, 2280, 2392, 3231, 4122, 4185
StyI CCWWGG 3 cut(s) 88, 1537, 3585
TaiI ACGT 3 cut(s) 230, 2725, 4131
TatI WGTACW 3 cut(s) 652, 886, 3748
TauI GCSGC 3 cut(s) 369, 736, 4322
Tru1I TTAA 7 cut(s) 1238, 1301, 1397, 2039, 3762, 4431, 4476
Tru9I TTAA 7 cut(s) 1238, 1301, 1397, 2039, 3762, 4431, 4476
TseFI GTSAC 7 cut(s) 1085, 1269, 1345, 1486, 2298, 2774, 4250
Tsp45I GTSAC 7 cut(s) 1085, 1269, 1345, 1486, 2298, 2774, 4250
Tth111I GACNNNGTC 1 cut(s) 4240
Van91I CCANNNNNTGG 2 cut(s) 794, 1474
VneI GTGCAC 1 cut(s) 3438
VpaK11BI GGWCC 3 cut(s) 120, 2383, 3658
XagI CCTNNNNNAGG 1 cut(s) 3351
XapI RAATTY 3 cut(s) 2137, 3380, 3671
XbaI TCTAGA 1 cut(s) 2959
XceI RCATGY 8 cut(s) 245, 659, 1007, 1969, 2846, 3170, 4209, 4288
XcmI CCANNNNNNNNNTGG 1 cut(s) 3838
XhoI CTCGAG 1 cut(s) 804
XmiI GTMKAC 1 cut(s) 1125
XmnI GAANNNNTTC 1 cut(s) 2322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.