Rw2G054810

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003475
Physical Location & Seq
Reverse (-)
82418057 .. 82425314
7258 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G054810.1

Sequence Viewer

Length: 4935 bp
ATGGCTCACGGAAAAGTCAGTCTCCCTCAAGATCTCCTCCCCTCGCCGATCTTCTCCGCCACCAAAGATGGAGCCTTGGAAGGAAATGCTGAGGGAAAGGCTCTAACGGGGTCACTTGATGAGTCAAAAGATCATTTAGCATCTGAGAGCAGCATACCTTTGTCACCACAATGGCTACATGCTAAATCAGTTGAGGCTAAGCCACTTGCAAGTGGAACATCAGGAGAAATCCATGCACCAGGGTCTTTGTCGCATGGTAACTCTATTGATTCCAATGCGAGAGACATTTGGCGTTCAGATGGTTCTCGGGACAGAAAAGATTGGAGGAGGAATGCACCTGATTTAGATGTTAACCGTCGCTGGCGTGAGGAGGAGAGAGAAACAGGCTTACTTGGTAGGAGGGATCGCAAAAAAGAAGATCGCCGTGTAGGAGTCACTTCAACTAGGGATATTGCTGAGAATCGAGCAGAAGATCATCGAGCAGGTGGCCCTTCTACTAGGGATGTTAGTGAGAATAAAATTTTGTCTTCTGATCGCTGGCATGAATCCCGGAGAGACAACAAATGGTCATCAAGATGGGGCCCTGAAGATAAAGACAAGGACTCTCGAATAGAGAAGACAGAACTTGAGAGAGAAGATGCTCATGTTGACAAGCAGTCTCTTGTCAGTAGCAATCGTGCAATTCCTGAACGTGATGGTGATTCTCGCGATAAATGGAGGCCACGCCATCGCATGGAGGTTCAGTCCGGGGGGCCAGCCCCATACCGTGCCGCACCGGGATTTGGGATGGCCAGAGGACAGGTGGAAAAAGTAGGATTTGCTTCTGGCCGAGGGAGGTCCAAGACTAATGGTAGCCTACAGATTGGAAAACCTTCCTCTGTATCCACTATCGGGTCTGTTCTGGATCAGAATCAAAGTGTATTGGGAAACGCTGGCCCCTTTAATGAGAAGTATCGTTACCCCAGGGGAAAGCTCCTTGATATCTATCGTAAGCAAAAGATAGAATCAACTTTCGATATTGTGCCTGATGGGATGGAGCATGTCTCTCCAATTACCCAAGTGGGCTCTATTGAGCCATTGGCCTTTTCTGCACCTCATGCAGAAGAAGAGGCTGTTATGGAAGATATATGGAAGGGAAGAATCACTAGCAGTGAAATGTTGTGCAGTTCCTCCAGAGAAAAGAATGTGTTAAATGATGATGGTAAAGGAACTAGTAATGCCTCCTTAAGAAAGGAAGATCAAAAGATTTCACTGCAGACTGAAGAAAATGCCCTATCTACTGGAGAGCCCATCTTAAACAATTCTTTTCCAGTTATCGGTACTGAGGTCTCAACTATCTGTGGCCCACGGACACATATTTTAAATGAATGGGTTGAAGGTGAGCGCAGAGTTCCTATTGTCACAGCAGTTGCTGATGGATCAAATCTGGTTGCTTCAAAGAACTATGATTGCAAAAGTGCTGGGGAGATTGATGGCCTCAGTAATAGTGTCGCTGAACTAAGAATTTCTGGATGCCAGGATGTGAGAGATTTTGGTCTTCCAAAGCATTCTATGTCGGAAAATTTTGAACCAGCTACTACTGTGATTGGTAGCCAGCTTCCTGATGATTCAAGTTCACTTTTTCACATTCCTTCTCTACAGCAAACCTCGAGTAGTGATCAATATGTCAAGAGCAACGAGAAGGCAGATCTGGAAAGGGTTGTCTCATTTGAGGATTTAAGTTTATGCTATCTTGATCCTCAAGGAAACATCCAGGGACCTTTCCTGGGGATCGACATAATTTCATGGTTTGATCAAGGATTCTTTGGAACTGACTTACTGGTGCGCTTGGCTGATGCCCCTAATGGTTCTCCTTTTCAAGAACTCGGTGAAATGATGCCAAATCTGAACACTAAATCTGGATTGGTCTCCAACTCCAATTTGCCTACCAACGTGGAATCATTTGGTGGTGTTGGAAGCATCCTGGAAGAGAGAAAAACTGCTCCTCACTATGAGGAGTCTAACATTCTAAATATCCAACACTGGACTTCATCTGGACTTGAACCTACCTTGAGTGATGATTTGCACTCGAGAATACCTAATAATGGTTACAACTCTGAACTCCAGTACCTTGATAATCAAAGGTTTCAAAATTACGTTGAAGAAGATGAGGAAATAGTGTTTCCCGGAAGGCCTAAAAGTAGCAGTGATTTTCCTCTGAGGAGACCTTCAGCTGATATTCAGGGCTCATTTTCAAATTCCCCAAGCCTTCCTTCCCTTTCAAATGAAGTTTCCGAAAGTGACCTGCCTAATCGCCAGGATGATAAGTTGCACCCTTTTGGCCTGTTGATGTCTGAGCTCAGAGGCAGCTCTCATTTAAGACCTGCTCAAGCTTCCAATGTGTCTTTAGGCATGGATGATCAGGCTCAGTTTAGAGATACCTTGTTTGAGGGAGGTATCTCCAAACTGAGTTGGTTGAATAATCCTAATATCCCAATGCATCAAGTAGTGCCTGATATGGAACATCTAATAGAACTACAGCGTCAAGAGCAGCAGCATCGCCTATTGGAGCTTCAGCAACAACGCCAGTTTGAGTTTCATCGTCAGCAGCAGCACGAGTTGGAATTTCAACAGCAGCAGCAGCAGCAGCGGCAATTGGAACTCCAGCAACAGCGCCAGTTGGAGCTACAGCAGCAGCGCCAGTTGGAACTTCACCACTTGGAGCTTCAGCAGCAGCACCAGTTGGAGCTACAGCTGCAGCGCCAGTTGGAGCTACAGCAACAGCGCCAGTTTGAGCTACAGAAACAGCGCCAGTTGGAGCTACAGCAACAACGCCAGTTGGAGCTTCAGCAACAGCAGCAACACCATTTGGAGCTTCAACAACAGCAGCAGCGGAAGCTGGAGCTTCAGCAGCTGCAGCGTCATCAGCAACAGCGGCAGTTGGAGTTTCAGCAGCAGCGCCAATTGGAGCTTCAGCAACAGCAGCATCAATTGGAGCTTCCGCCTCAGCACCACTTGCAGCAGCACCTTCGCCATCAACAAAAATTGCAGCAACAACAGCAGTCCCAAGCCGAACAATTGCTCCTCGAACAATTATTGAATCGACAAATGTCTGATGGTTATGGGCAGTGGAAGATGGATGCTACACGAGCCAACCTTCATGATCAGTTACATTCCAGTAACCACCTTCCAAATGATTTGCACCATGTATCTCATTCATCAAGGCACGATCCCTCGCTAGAGCAGATCATCCAAGCAACTATGGGCGAGGGTGCACTCCAAGGACAGACTGATTTTTTTGACCTCATATCGCAGGCCAAGCAAGGGAATAGGAACACTTCAGAGCTGCCACTTCGTCTTAATGAACAAGAGTTGCAAGCACAGCAATTATCTTTGGCATTTAGAGAGCAGCAGCAGATAGAAGGGGAAAGGCGTATTGGTGGGCCTTGGTTTGCAGATGAAGCAGGTCCTTTTTTGAGGGATCCCGTTGGGCATCACCAGGCACACATGTTGGGGCATAATTCTTCAGAAAATTACCAGCAACAGCAGAGGTTTCCATCCAATGAACAGGAACCAAGCCATCACGATTGGAGTCATGCCTCTAAGGAGAGACCTCAGGGAGGGTTTTATGAATCTAACTCTATGGAATTTGATAGATTCTCTGTTCCTGCTGGTTCTCATGGAATGAACTTGGATATTGCAAACATACGTGGACAAGATCTAGATGTGAGGGAACAATATCGGCTTAATTCCTCTATTGATGAGCGTGGATCTCTATCATCTGGCGTAACCTCTCACCGTCGTTTGGTTTCTGATGAGTTTTATGCTTCTCATCCAGTTACTTTTGAAAGCTTGCCATCTGGGAACAATGGGCAGCTAGAAAGTAGCTGCACTGAAGCAGTAATGCCTTATTTGCATCTTGATGCTGAGCAAAAACGACGAGACACGGAAGACACCATTGCTTTTGCTGCTTCAAATAGATTGGCTTCAACTAACGGTGATAAGGAACACTCCAAACAAATTTTGATGGACCTTCATCAAAAACTTGGTCATCAGCCTACACAGTCATCAGAAGTTGATTATCAGCATCACCTATCCTCATCCAGAAGTCAGGGAGGCTCTGTACATCTTCCTTTAAATCTTCTTCCAGATCAGTCAGTTGGTATGAGCAACTGCTTCACAGAAGGCCCACAGAGTTCAAATTTTAGTCTCTTGTTGCAAGATCAGTTGGGTAGTTATGGTATGAATGAGCAACCAAGTAATCTGGCAAGAAGATCTGATTCCAGAGCATTTATGGACGCTCAATTCTTGTCAGGAACCAGGGATGCTCCTCATGAAGGCAACATGGCAAACCAACAAGACACAGCCATTGAGAGCGGGGAACTACCTAGTAATACCCATAGCAGGCATAGCTCACTGAGCAGTGCTGGTGGATGTGGGGGTTTTCACAGTTCTGAGATGGGATTAGATAAATCACTTGGAGAAGATGTTTCTGATGGGAGGCTGCCGTCTACTATAACTAAAGGGTTTGACAGTGCTACACAGAGACGCCGCGTCTTATCTTCACAGGATGTTTCGTCTGAGTCTGCACTCTCTCTGCCTGTCAAGCCAAGAAGCTCCATAAGCCTCAGAACTTCTGAAAAAAATCCTGCTGCCACACTGGTCTCTGAATCTCGGGCATCTAGGAAAAATGATGCACAGTTTAGGAGGACTTCATCTTACAGTGATGCTGGTGTCTCTGAGACATCCTTCATAGATGTGCTGAAGAAACCAGTCATTCCAGAGGCTGACGTAGCCAACAGGGGTGCAGTGGAGTCATCCGATGGAGGTGGCCAAGTGGGGCGAACTGGCAAGAAGAAAGGCAAGAAAGGAAGACAGATCGATCCTGCTCTCCTTGGCTTTAAGGTATCCAGCAACCGGATCATGATGGGTGAGATCCATCGCCTTGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1644

Amino Acids

184.6

Weight (kDa)

5.66

Isoelectric Point (pI)

62.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 577 - 621 1.4e-10 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 473
Acc36I ACCTGC 4 cut(s) 473, 2292, 2371, 3434
AccB7I CCANNNNNTGG 1 cut(s) 733
AccBSI CCGCTC 1 cut(s) 4356
AccI GTMKAC 1 cut(s) 4490
AccII CGCG 2 cut(s) 708, 4533
AciI CCGC 8 cut(s) 57, 771, 2629, 2872, 2914, 2981, 4356, 4531
AcoI YGGCCR 3 cut(s) 789, 826, 4810
AcsI RAATTY 8 cut(s) 519, 1503, 1561, 2236, 2603, 3626, 3999, 4180
AcyI GRCGYC 1 cut(s) 4528
AfaI GTAC 3 cut(s) 1321, 2108, 4104
AflII CTTAAG 1 cut(s) 1225
AflIII ACRYGT 1 cut(s) 3486
AhdI GACNNNNNGTC 2 cut(s) 655, 4532
AhlI ACTAGT 1 cut(s) 1211
AjnI CCWGG 9 cut(s) 238, 962, 1515, 1752, 1764, 1962, 2295, 3477, 4298
AjuI GAANNNNNNNTTGG 6 cut(s) 2236, 2268, 2934, 2966, 3399, 3431
AloI GAACNNNNNNTCC 2 cut(s) 3045, 3077
Alw21I GWGCWC 2 cut(s) 2340, 3256
Alw44I GTGCAC 1 cut(s) 3252
AlwNI CAGNNNCTG 3 cut(s) 1412, 2893, 4766
Ama87I CYCGRG 4 cut(s) 306, 1648, 2068, 4653
ApaI GGGCCC 1 cut(s) 584
ApaLI GTGCAC 1 cut(s) 3252
ApoI RAATTY 8 cut(s) 519, 1503, 1561, 2236, 2603, 3626, 3999, 4180
ArsI GACNNNNNNTTYG 2 cut(s) 4012, 4044
Asp700I GAANNNNTTC 1 cut(s) 871
AspLEI GCGC 8 cut(s) 1386, 1827, 2655, 2679, 2742, 2766, 2790, 2940
AsuC2I CCSGG 4 cut(s) 550, 748, 777, 2166
AvaI CYCGRG 4 cut(s) 306, 1648, 2068, 4653
AvaII GGWCC 4 cut(s) 837, 1757, 3446, 4009
AxyI CCTNAGG 1 cut(s) 3594
BaeGI GKGCMC 2 cut(s) 584, 3256
BaeI ACNNNNGTAYC 4 cut(s) 1311, 1344, 2090, 2123
BalI TGGCCA 2 cut(s) 791, 4812
BamHI GGATCC 1 cut(s) 3460
BanII GRGCYC 5 cut(s) 584, 1067, 1290, 2228, 2340
BarI GAAGNNNNNNTAC 2 cut(s) 941, 973
BauI CACGAG 2 cut(s) 2594, 3126
BbsI GAAGAC 5 cut(s) 519, 623, 1529, 3936, 4858
Bbv12I GWGCWC 2 cut(s) 2340, 3256
BbvCI CCTCAGC 2 cut(s) 90, 2985
BceAI ACGGC 2 cut(s) 408, 4471
BcgI CGANNNNNNTGC 4 cut(s) 2519, 2553, 2990, 3024
BciT130I CCWGG 9 cut(s) 240, 964, 1517, 1754, 1766, 1964, 2297, 3479, 4300
BciVI GTATCC 2 cut(s) 892, 4897
BclI TGATCA 4 cut(s) 1657, 1792, 2398, 3142
BcnI CCSGG 4 cut(s) 550, 748, 777, 2166
BcuI ACTAGT 1 cut(s) 1211
BfaI CTAG 9 cut(s) 444, 498, 1146, 1212, 3218, 3701, 3857, 4368, 4662
BfoI RGCGCY 6 cut(s) 2656, 2680, 2743, 2767, 2791, 2941
BfrI CTTAAG 1 cut(s) 1225
BfuAI ACCTGC 4 cut(s) 473, 2292, 2371, 3434
BfuI GTATCC 2 cut(s) 892, 4897
BglII AGATCT 4 cut(s) 31, 1687, 3697, 4253
BlpI GCTNAGC 2 cut(s) 198, 3906
Bme18I GGWCC 4 cut(s) 837, 1757, 3446, 4009
BmeRI GACNNNNNGTC 2 cut(s) 655, 4532
BmeT110I CYCGRG 4 cut(s) 306, 1648, 2068, 4653
BmiI GGNNCC 9 cut(s) 73, 581, 582, 753, 937, 1758, 3462, 3552, 4297
BoxI GACNNNNGTC 1 cut(s) 3088
BpiI GAAGAC 5 cut(s) 519, 623, 1529, 3936, 4858
BplI GAGNNNNNCTC 2 cut(s) 1028, 1060
BpmI CTGGAG 5 cut(s) 1156, 1302, 2087, 2627, 2900
Bpu10I CCTNAGC 2 cut(s) 90, 2985
Bpu1102I GCTNAGC 2 cut(s) 198, 3906
BpuEI CTTGAG 5 cut(s) 12, 647, 1725, 2071, 2352
BpuMI CCSGG 4 cut(s) 550, 748, 777, 2166
Bsa29I ATCGAT 1 cut(s) 4860
BsaAI YACGTR 1 cut(s) 3689
BsaBI GATNNNNATC 3 cut(s) 909, 984, 3754
BsaHI GRCGYC 1 cut(s) 4528
BsaI GGTCTC 5 cut(s) 1333, 1912, 2197, 3583, 4648
BsaWI WCCGGW 1 cut(s) 4896
BsaXI ACNNNNNCTCC 8 cut(s) 728, 758, 3045, 3075, 4086, 4116, 4452, 4482
Bse21I CCTNAGG 1 cut(s) 3594
Bse3DI GCAATG 1 cut(s) 3936
Bse8I GATNNNNATC 3 cut(s) 909, 984, 3754
BseBI CCWGG 9 cut(s) 240, 964, 1517, 1754, 1766, 1964, 2297, 3479, 4300
BseCI ATCGAT 1 cut(s) 4860
BseJI GATNNNNATC 3 cut(s) 909, 984, 3754
BseMI GCAATG 1 cut(s) 3936
BseRI GAGGAG 9 cut(s) 26, 340, 383, 386, 1974, 2009, 2215, 3053, 4299
BseSI GKGCMC 2 cut(s) 584, 3256
BseYI CCCAGC 1 cut(s) 1460
BsgI GTGCAG 5 cut(s) 1074, 1183, 3853, 4551, 4806
Bsh1236I CGCG 2 cut(s) 708, 4533
BshVI ATCGAT 1 cut(s) 4860
BsiHKAI GWGCWC 2 cut(s) 2340, 3256
BsiHKCI CYCGRG 4 cut(s) 306, 1648, 2068, 4653
BsiSI CCGG 5 cut(s) 550, 747, 776, 2166, 4897
BslFI GGGAC 3 cut(s) 323, 1770, 3028
BsmBI CGTCTC 1 cut(s) 4519
BsmFI GGGAC 3 cut(s) 323, 1770, 3028
BsmI GAATGC 2 cut(s) 337, 1546
Bso31I GGTCTC 5 cut(s) 1333, 1912, 2197, 3583, 4648
BsoBI CYCGRG 4 cut(s) 306, 1648, 2068, 4653
Bsp120I GGGCCC 1 cut(s) 580
Bsp1286I GDGCHC 6 cut(s) 584, 1067, 1290, 2228, 2340, 3256
Bsp1407I TGTACA 1 cut(s) 4102
Bsp1720I GCTNAGC 2 cut(s) 198, 3906
Bsp68I TCGCGA 1 cut(s) 708
BspACI CCGC 8 cut(s) 57, 771, 2629, 2872, 2914, 2981, 4356, 4531
BspDI ATCGAT 1 cut(s) 4860
BspFNI CGCG 2 cut(s) 708, 4533
BspHI TCATGA 3 cut(s) 3139, 4312, 4902
BspLI GGNNCC 9 cut(s) 73, 581, 582, 753, 937, 1758, 3462, 3552, 4297
BspMAI CTGCAG 3 cut(s) 1257, 2739, 2898
BspMI ACCTGC 4 cut(s) 473, 2292, 2371, 3434
BspTI CTTAAG 1 cut(s) 1225
BspTNI GGTCTC 5 cut(s) 1333, 1912, 2197, 3583, 4648
BsrBI CCGCTC 1 cut(s) 4356
BsrDI GCAATG 1 cut(s) 3936
BsrGI TGTACA 1 cut(s) 4102
BssNI GRCGYC 1 cut(s) 4528
BssSI CACGAG 2 cut(s) 2594, 3126
BssT1I CCWWGG 4 cut(s) 75, 3259, 3425, 4873
Bst2BI CACGAG 2 cut(s) 2594, 3126
Bst2UI CCWGG 9 cut(s) 240, 964, 1517, 1754, 1766, 1964, 2297, 3479, 4300
Bst6I CTCTTC 2 cut(s) 1101, 1962
BstACI GRCGYC 1 cut(s) 4528
BstAFI CTTAAG 1 cut(s) 1225
BstAPI GCANNNNNTGC 2 cut(s) 1097, 2995
BstAUI TGTACA 1 cut(s) 4102
BstBAI YACGTR 1 cut(s) 3689
BstC8I GCNNGC 9 cut(s) 362, 539, 756, 934, 1595, 3294, 3357, 3833, 4385
BstDSI CCRYGG 1 cut(s) 1346
BstENI CCTNNNNNAGG 2 cut(s) 3597, 4314
BstFNI CGCG 2 cut(s) 708, 4533
BstH2I RGCGCY 6 cut(s) 2656, 2680, 2743, 2767, 2791, 2941
BstHHI GCGC 8 cut(s) 1386, 1827, 2655, 2679, 2742, 2766, 2790, 2940
BstNI CCWGG 9 cut(s) 240, 964, 1517, 1754, 1766, 1964, 2297, 3479, 4300
BstNSI RCATGY 3 cut(s) 182, 1043, 3490
BstPAI GACNNNNGTC 1 cut(s) 3088
BstSLI GKGCMC 2 cut(s) 584, 3256
BstUI CGCG 2 cut(s) 708, 4533
BstV2I GAAGAC 5 cut(s) 519, 623, 1529, 3936, 4858
BstX2I RGATCY 7 cut(s) 31, 1687, 3460, 3697, 3749, 4253, 4914
BstYI RGATCY 7 cut(s) 31, 1687, 3460, 3697, 3749, 4253, 4914
Bsu15I ATCGAT 1 cut(s) 4860
Bsu36I CCTNAGG 1 cut(s) 3594
BsuI GTATCC 2 cut(s) 892, 4897
BsuTUI ATCGAT 1 cut(s) 4860
BtgI CCRYGG 1 cut(s) 1346
BtgZI GCGATG 3 cut(s) 713, 2522, 4904
BtsI GCAGTG 6 cut(s) 1156, 1250, 2191, 3113, 4408, 4794
BtuMI TCGCGA 1 cut(s) 708
BveI ACCTGC 4 cut(s) 473, 2292, 2371, 3434
Cac8I GCNNGC 9 cut(s) 362, 539, 756, 934, 1595, 3294, 3357, 3833, 4385
CaiI CAGNNNCTG 3 cut(s) 1412, 2893, 4766
CciI TCATGA 3 cut(s) 3139, 4312, 4902
CfoI GCGC 8 cut(s) 1386, 1827, 2655, 2679, 2742, 2766, 2790, 2940
ClaI ATCGAT 1 cut(s) 4860
CseI GACGC 5 cut(s) 2510, 2888, 4286, 4522, 4536
Csp6I GTAC 3 cut(s) 1320, 2107, 4103
CviQI GTAC 3 cut(s) 1320, 2107, 4103
DraI TTTAAA 2 cut(s) 1362, 4116
DriI GACNNNNNGTC 2 cut(s) 655, 4532
EaeI YGGCCR 3 cut(s) 789, 826, 4810
Eam1104I CTCTTC 2 cut(s) 1101, 1962
Eam1105I GACNNNNNGTC 2 cut(s) 655, 4532
EarI CTCTTC 2 cut(s) 1101, 1962
EciI GGCGGA 2 cut(s) 46, 2970
Ecl136II GAGCTC 1 cut(s) 2338
Eco130I CCWWGG 4 cut(s) 75, 3259, 3425, 4873
Eco147I AGGCCT 1 cut(s) 2173
Eco24I GRGCYC 5 cut(s) 584, 1067, 1290, 2228, 2340
Eco31I GGTCTC 5 cut(s) 1333, 1912, 2197, 3583, 4648
Eco32I GATATC 1 cut(s) 982
Eco47I GGWCC 4 cut(s) 837, 1757, 3446, 4009
Eco53kI GAGCTC 1 cut(s) 2338
Eco81I CCTNAGG 1 cut(s) 3594
Eco88I CYCGRG 4 cut(s) 306, 1648, 2068, 4653
EcoICRI GAGCTC 1 cut(s) 2338
EcoNI CCTNNNNNAGG 2 cut(s) 3597, 4314
EcoO109I RGGNCCY 4 cut(s) 580, 581, 1757, 3446
EcoRII CCWGG 9 cut(s) 238, 962, 1515, 1752, 1764, 1962, 2295, 3477, 4298
EcoRV GATATC 1 cut(s) 982
EcoT14I CCWWGG 4 cut(s) 75, 3259, 3425, 4873
EcoT22I ATGCAT 1 cut(s) 2481
EcoT38I GRGCYC 5 cut(s) 584, 1067, 1290, 2228, 2340
ErhI CCWWGG 4 cut(s) 75, 3259, 3425, 4873
Esp3I CGTCTC 1 cut(s) 4519
FalI AAGNNNNNCTT 2 cut(s) 2236, 2268
FaqI GGGAC 3 cut(s) 323, 1770, 3028
FauI CCCGC 1 cut(s) 4349
FbaI TGATCA 4 cut(s) 1657, 1792, 2398, 3142
FblI GTMKAC 1 cut(s) 4490
FriOI GRGCYC 5 cut(s) 584, 1067, 1290, 2228, 2340
FspBI CTAG 9 cut(s) 444, 498, 1146, 1212, 3218, 3701, 3857, 4368, 4662
GlaI GCGC 8 cut(s) 1385, 1826, 2654, 2678, 2741, 2765, 2789, 2939
GsaI CCCAGC 1 cut(s) 1464
GsuI CTGGAG 5 cut(s) 1156, 1302, 2087, 2627, 2900
HaeII RGCGCY 6 cut(s) 2656, 2680, 2743, 2767, 2791, 2941
HapII CCGG 5 cut(s) 550, 747, 776, 2166, 4897
HgaI GACGC 5 cut(s) 2510, 2888, 4286, 4522, 4536
HhaI GCGC 8 cut(s) 1386, 1827, 2655, 2679, 2742, 2766, 2790, 2940
Hin1I GRCGYC 1 cut(s) 4528
Hin6I GCGC 8 cut(s) 1384, 1825, 2653, 2677, 2740, 2764, 2788, 2938
HinP1I GCGC 8 cut(s) 1384, 1825, 2653, 2677, 2740, 2764, 2788, 2938
HincII GTYRAC 2 cut(s) 352, 649
HindII GTYRAC 2 cut(s) 352, 649
HindIII AAGCTT 2 cut(s) 2370, 3829
HpaI GTTAAC 1 cut(s) 352
HpaII CCGG 5 cut(s) 550, 747, 776, 2166, 4897
Hpy166II GTNNAC 6 cut(s) 352, 649, 1616, 3254, 3692, 4491
Hpy8I GTNNAC 6 cut(s) 352, 649, 1616, 3254, 3692, 4491
Hpy99I CGWCG 3 cut(s) 360, 3783, 3921
HpyCH4IV ACGT 5 cut(s) 691, 1932, 2136, 3688, 4770
HpySE526I ACGT 5 cut(s) 691, 1932, 2136, 3688, 4770
Hsp92I GRCGYC 1 cut(s) 4528
HspAI GCGC 8 cut(s) 1384, 1825, 2653, 2677, 2740, 2764, 2788, 2938
Ksp22I TGATCA 4 cut(s) 1657, 1792, 2398, 3142
KspAI GTTAAC 1 cut(s) 352
MaeI CTAG 9 cut(s) 444, 498, 1146, 1212, 3218, 3701, 3857, 4368, 4662
MaeII ACGT 5 cut(s) 691, 1932, 2136, 3688, 4770
MbiI CCGCTC 1 cut(s) 4356
MfeI CAATTG 4 cut(s) 2633, 2942, 2969, 3056
MflI RGATCY 7 cut(s) 31, 1687, 3460, 3697, 3749, 4253, 4914
MhlI GDGCHC 6 cut(s) 584, 1067, 1290, 2228, 2340, 3256
MlsI TGGCCA 2 cut(s) 791, 4812
MluNI TGGCCA 2 cut(s) 791, 4812
MlyI GAGTC 7 cut(s) 131, 441, 596, 2006, 3580, 4571, 4802
Mox20I TGGCCA 2 cut(s) 791, 4812
Mph1103I ATGCAT 1 cut(s) 2481
MroXI GAANNNNTTC 1 cut(s) 871
MscI TGGCCA 2 cut(s) 791, 4812
MslI CAYNNNNRTG 4 cut(s) 169, 574, 3900, 4736
Msp20I TGGCCA 2 cut(s) 791, 4812
MspA1I CMGCKG 6 cut(s) 2213, 2629, 2734, 2872, 2893, 2914
MspCI CTTAAG 1 cut(s) 1225
MspI CCGG 5 cut(s) 550, 747, 776, 2166, 4897
MunI CAATTG 4 cut(s) 2633, 2942, 2969, 3056
Mva1269I GAATGC 2 cut(s) 337, 1546
MvaI CCWGG 9 cut(s) 240, 964, 1517, 1754, 1766, 1964, 2297, 3479, 4300
MvnI CGCG 2 cut(s) 708, 4533
NciI CCSGG 4 cut(s) 550, 748, 777, 2166
NlaIV GGNNCC 9 cut(s) 73, 581, 582, 753, 937, 1758, 3462, 3552, 4297
NmeAIII GCCGAG 1 cut(s) 854
NmuCI GTSAC 5 cut(s) 111, 162, 433, 1399, 2279
NruI TCGCGA 1 cut(s) 708
NsiI ATGCAT 1 cut(s) 2481
NspI RCATGY 3 cut(s) 182, 1043, 3490
PaeR7I CTCGAG 2 cut(s) 1648, 2068
PagI TCATGA 3 cut(s) 3139, 4312, 4902
PaqCI CACCTGC 1 cut(s) 473
PasI CCCWGGG 1 cut(s) 963
PceI AGGCCT 1 cut(s) 2173
PciI ACATGT 1 cut(s) 3486
PctI GAATGC 2 cut(s) 337, 1546
PdmI GAANNNNTTC 1 cut(s) 871
PflMI CCANNNNNTGG 1 cut(s) 733
PfoI TCCNGGA 3 cut(s) 548, 1962, 2164
PleI GAGTC 7 cut(s) 130, 440, 596, 2005, 3579, 4570, 4801
PpsI GAGTC 7 cut(s) 130, 440, 596, 2005, 3579, 4570, 4801
Ppu21I YACGTR 1 cut(s) 3689
PpuMI RGGWCCY 2 cut(s) 1757, 3446
PscI ACATGT 1 cut(s) 3486
PshAI GACNNNNGTC 1 cut(s) 3088
Psp124BI GAGCTC 1 cut(s) 2340
Psp5II RGGWCCY 2 cut(s) 1757, 3446
Psp6I CCWGG 9 cut(s) 238, 962, 1515, 1752, 1764, 1962, 2295, 3477, 4298
PspFI CCCAGC 1 cut(s) 1460
PspGI CCWGG 9 cut(s) 238, 962, 1515, 1752, 1764, 1962, 2295, 3477, 4298
PspN4I GGNNCC 9 cut(s) 73, 581, 582, 753, 937, 1758, 3462, 3552, 4297
PspOMI GGGCCC 1 cut(s) 580
PspPPI RGGWCCY 2 cut(s) 1757, 3446
PspXI VCTCGAGB 1 cut(s) 1648
PstI CTGCAG 3 cut(s) 1257, 2739, 2898
PstNI CAGNNNCTG 3 cut(s) 1412, 2893, 4766
PsuI RGATCY 7 cut(s) 31, 1687, 3460, 3697, 3749, 4253, 4914
PvuII CAGCTG 3 cut(s) 2213, 2734, 2893
RruI TCGCGA 1 cut(s) 708
RsaI GTAC 3 cut(s) 1321, 2108, 4104
RsaNI GTAC 3 cut(s) 1320, 2107, 4103
RseI CAYNNNNRTG 4 cut(s) 169, 574, 3900, 4736
SacI GAGCTC 1 cut(s) 2340
SchI GAGTC 7 cut(s) 131, 441, 596, 2006, 3580, 4571, 4802
SduI GDGCHC 6 cut(s) 584, 1067, 1290, 2228, 2340, 3256
Sfr274I CTCGAG 2 cut(s) 1648, 2068
SinI GGWCC 4 cut(s) 837, 1757, 3446, 4009
SlaI CTCGAG 2 cut(s) 1648, 2068
SmiMI CAYNNNNRTG 4 cut(s) 169, 574, 3900, 4736
SmlI CTYRAG 8 cut(s) 27, 626, 1225, 1648, 1740, 2050, 2068, 2367
SmoI CTYRAG 8 cut(s) 27, 626, 1225, 1648, 1740, 2050, 2068, 2367
SpeI ACTAGT 1 cut(s) 1211
SseBI AGGCCT 1 cut(s) 2173
SsiI CCGC 8 cut(s) 57, 771, 2629, 2872, 2914, 2981, 4356, 4531
SspMI CTAG 9 cut(s) 444, 498, 1146, 1212, 3218, 3701, 3857, 4368, 4662
SstI GAGCTC 1 cut(s) 2340
StuI AGGCCT 1 cut(s) 2173
StyI CCWWGG 4 cut(s) 75, 3259, 3425, 4873
TaiI ACGT 5 cut(s) 694, 1935, 2139, 3691, 4773
TatI WGTACW 1 cut(s) 4102
TauI GCSGC 4 cut(s) 773, 2632, 2917, 4533
TseFI GTSAC 5 cut(s) 111, 162, 433, 1399, 2279
Tsp45I GTSAC 5 cut(s) 111, 162, 433, 1399, 2279
TspGWI ACGGA 3 cut(s) 24, 1363, 3941
Van91I CCANNNNNTGG 1 cut(s) 733
Vha464I CTTAAG 1 cut(s) 1225
VneI GTGCAC 1 cut(s) 3252
VpaK11BI GGWCC 4 cut(s) 837, 1757, 3446, 4009
XagI CCTNNNNNAGG 2 cut(s) 3597, 4314
XapI RAATTY 8 cut(s) 519, 1503, 1561, 2236, 2603, 3626, 3999, 4180
XbaI TCTAGA 1 cut(s) 3700
XceI RCATGY 3 cut(s) 182, 1043, 3490
XcmI CCANNNNNNNNNTGG 2 cut(s) 799, 4270
XhoI CTCGAG 2 cut(s) 1648, 2068
XmiI GTMKAC 1 cut(s) 4490
XmnI GAANNNNTTC 1 cut(s) 871
XspI CTAG 9 cut(s) 444, 498, 1146, 1212, 3218, 3701, 3857, 4368, 4662
Zsp2I ATGCAT 1 cut(s) 2481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.