RchiOBHm_Chr2g0176001

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003475
Physical Location & Seq
Reverse (-)
87970703 .. 87978564
7862 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54303

Sequence Viewer

Length: 4923 bp
ATGGCTCACGGAAAAGTCAGTCTCCCTCAAGATCTCCTCCCCTCGCCGATCTTCTCCGCCACCAAAGATGGAGCCTTGGAAGGAAATGCTGAGGGAAAGGCTCTAACGGGGTCACTTGATGATTCAAAAGATCATTTAGCATCTGAGAGCAGCATACCTTTGTCACCACAATGGCTACATGCTAAATCAGTTGAGGCTAAGCCACTTGCAAGTGGAACATCAGGAGAAATCCATGCACCAGGGTCTTTGTCGCATGGTAACTCTATTGATTCCAATGCGAGAGACATTTGGCGTTCAGATGGTTCTCGGGACAGAAAAGATTGGAGGAGGAATGCACCTGATTTAGATGTTAACCGTCGCTGGCGTGAGGAGGAGAGAGAAACAGGCTTACTTGGTAGGAGGGATCGCAAAAAAGAAGATCGCCGTGTAGGAGTCACTTCAACTAGGGATATTGCTGAGAATCGAGCAGAAGATCATCGAGCAGGTGGCCCTTCTACTAGGGATGTTAGTGAGAATAAAATTTTGTCTTCTGATCGCTGGCATGAATCCCGGAGAGACAACAAATGGTCATCAAGGTGGGGCCCTGAAGATAAAGACAAGGACTCTCGAATAGAGAAGACAGAACTTGAGAGAGAAGATGCTCATGTTGACAAGCAGTCTCTTGTCAGTAGCAATCGTGCAATTCCTGAACGTGATGGTGATTCTCGCGATAAATGGAGGCCACGCCATCGCATGGAGGTTCAGTCCGGGGGGCCAGCCCCATACCGTGCCGCACCGGGATTTGGGATGGCCAGAGGACAGGTGGAAAAAGTAGGATTTGCTTCTGGCCGAGGGAGGTCCAAGACTAATGGTAGCCTACAGATTGGAAAACCTTCCTCTGTATCCACTATCGGGTCTGTTCTGGATCAGAATCAAAGTGTATTGGGAAACACTGGCCCCTTTAATGAGAAGTATCGTTACCCCAGGGGAAAGCTCCTTGATATCTATCGTAAGCAAAAGATAGAATCAACTTTCGATATTGTGCCTGATGGGATGGAGCATGTCTCTCCAATTACCCAAGTGGGCTCTATTGAGCCATTGGCCTTTTCTGCACCTCATGCAGAAGAAGAGGCTGTTATGGAAGATATATGGAAGGGAAGAATCACTAGCAGTGAAATGTTGTGCAGTTCCTCCAGAGAAAAGAATGTGTTAAATGATGATGGAACTAGTAATGCCTCCTTAAGAAAGGAAGATCAAAAGATTTCACTGCAGACTGAAGAAAATGCCCTATCTACTGGAGAGCCCATCTTAAACAATTCTTTTCCAGTTATCGGTACTGAGGTCTCAACTATCTGTGGCCCACGGACACATATTTTAAATGAATGGGTTGAAGGTGAGCGCAGAGTTCCTATTGTCACAGCAGTTGCTGATGGATCAAATCTGGTTGCTTCAAAGAACTATGATTGCAAAAGTGCTGGGGAGATTGATGGCCTCAGTAATAGTGTCGCTGAACTAAGAATTTCTGGATGCCAGGATGTGAGAGATTTTGGTCTTCCAAAGCATTCTATGTCGGAAAATTTTGAACCAGCTACTACTGTGATTGGTAGCCAGCTTCCTGATGATTCAAGTTCACTTTTTCACATTCCTTCTCTACAGCAAACCTCGAGTAGTGATCAATATGTCAAGAGCAACGAGAAGGCAGATCTGGAAAGGGTTGTCTCATTTGAGGATTTAAGTTTATGCTATCTTGATCCTCAAGGAAACATCCAGGGACCTTTCCTGGGGATCGACATAATTTCATGGTTTGATCAAGGATTCTTTGGAACTGACTTACTGGTGCGCTTGGCTGATGCTCCTAATGGTTCTCCTTTTCAAGAACTCGGTGAAATGATGCCAAATCTGAACACTAAATCTGGATTGGTCTCCAACTCCAATTTGCCTACCAACATGGAATCATTTGGTGGTGTTGGAAGCATCCTGGAAGAGAGAAAAACTGCTCCTCACTATGAGGAGTCTAACATTCTAAATATCCAACACTGGACTTCATCTGGACTTGAACCTACCTTGAGTGATGATTTGCACTCGAGAATACCTAATAATGGTTACAACTCTGAACTCCAGTACCTTGATAATCAAAGGTTTCAAAATTACGTTGAAGAAGATGAGGAAATAGTGTTTCCCGGAAGGCCTAAAAGTAGCAGTGATTTTCCTCTGAGGAGACCTTCAGCTGATATTCAGGGCTCATTTTCAAATTCCCCAAGCCTTCCTTCCCTTTCAAATGAAGTTTCCGAAAGTGACCTGCCTAATCGCCAGGATGATAAGTTGCACCCTTTTGGCCTGTTGATGTCTGAGCTCAGAGGCAGCTCTCATTTAAGACCTGCTCAAGCTTCCAATGTGTCTTTAGGCATGGATGATCAGGCTCAGTTTAGAGATACCTTGTTTGAGGGAGGTATCTCCAAACTGAGTTGGTTGAATAATCCTAATATCCCAATGCATCAAGTAGTGCCTGATATGGAACATCTAATAGAACTACAGCGTCAAGAGCAGCAGCATCGCCTATTGGAGCTTCAGCAACAACGCCAGTTTGAGTTTCATCGTCAGCAGCAGCACGAGTTGGAATTTCAACAGCAGCAGCAGCGGCAATTGGAACTCCAGCAACAGCGCCAGTTGGAGCTACAGCAGCAGCGCCAGTTGGAACTTCACCACTTGGAGCTTCAGCAGCAGCACCAGTTGGAGCTACAGCTGCAGCGCCAGTTGGAGCTACAGCAACAGCGCCAGTTTGAGCTACAGCAACAGCGCCAGTTGGAGCTACAGCAACAACGCCAGTTGGAGCTTCAGCAACAGCAGCAACACCATTTGGAGCTTCAACAACAGCAGCAGCGGAAGCTGGAGCTTCAGCAGCTGCAGCGTCATCAGCAACAGCGGCAGTTGGAGTTTCAGCAGCAGCGCCAATTGGAGCTTCAGCAACAGCAGCATCAATTGGAGCTTCCGCCTCAGCACCACTTGCAGCAGCACCTTCGCCATCAACAAAAATTGCAGCAACAACAGCAGTCCCAAGCCGAACAATTGCTCCTCGAACAATTATTGAATCGACAAATGTCTGATGGTTATGGGCAGTGGAAGATGGATGCTACACGAGCCAACCTTCATGATCAGTTACATTCCAGTAACCACCTTCCAAATGATTTGCACCATGTATCTCATTCATCAAGGCACGATCCCTCGCTAGAGCAGATCATCCAAGCAACTATGGGCGAGGGTGCACTCCAAGGACAGACTGATTTTTTTGACCTCATATCGCAGGCCAAGCAAGGGAATAGGAACACTTCAGAGCTGCCACTTCGTCTTAATGAACAAGAGTTGCAAGCACAGCAATTATCTTTGGCATTTAGAGAGCAGCAGCAGATAGAAGGGGAAAGGCGTATTGGTGGGCCTTGGTTTGCAGATGAAGCAGGTCCTTTTTTGAGGGATCCCGTTGGGCATCACCAGGCACACATGTTGGGGCATAATTCTTCAGAAAATTACCAGCAACAGCAGAGGTTTCCATCCAATGAACAGGAACCAAGCCATAACGATTGGAGTCATGCCTCTAAGGAGAGACCTCAGGGAGGGTTTTATGAATCTAACTCTATGGAATTTGATAGATTCTCTGTTCCTGCTGGTTCTCATGGAATGAACTTGGATATTGCAAACATACGTGGACAAGATCTAGAGGTGAGGGAACAATATCGGCTTAATTCCTCTATTGATGAGCGTGGATCTCTATCATCTGGCGTAACCTCTCACCGTCGTTTGGTTTCTGATGAGTTTTATGCTTCTCATCCAGTTACTTTTGAAAGCTTGCCATCTGGGAACAATGGGCAGCTAGAAAGTAGCTGCACTGAAGCAGTAATGCCTTATTTGCATCTTGATGCTGAGCAAAAACGACGAGACACGGAAGACACCATTGCTTTTACTGCTTCAAATAGGTTGGCTTCAACTAACGGTGATAAGGAACACTCCAAACAAATTTTGATGGACCTTCATCAAAAACTTGGTCATCAGCCTACACAGTCATCAGAAGTTGATTATCAGCATCACTTATCCTCATGCAGAAGTCAGGGAGGCTCTGTACATCTTCCTTTAAATCTTCTTCCAGATCAGTCAGTTGGTATGAGCAACTGCTTCACAGAAGGCCCACAGAGTTCAAATTTTAGTCTCTTGTTGCAAGATCAGTTGGGTAGTTATGGTATGAATGAGCAACCAAGTAATCTGGCAAGAAGATCTGATTCCAGAGCATTTATGGACGCTCAATTCTTGTCAGGAACCAGGGATGCTCCTCATGAAGGCAACATGGCAAACCAACAAGACACAGCCATTGAGAGCGGGGAACTACCTAGTAATACCCATAGCAGGCATAGCTCACTGAGCAGTGCTGGTGGATGTGGGGGTTTTCACAGCTCTGAGATGGGATTAGATAAATCACTTGGAGAAGATGTTTCTGATGGGAGGCTGCCGTCTACTATAACTAAAGGGTTTGACAGTGCTACACAGAGACGCCGCGTCTTATCTTCACAGGATGTTTCGTCTGAGTCTGCACTCTCTCTGCCTGTCAAGCCAAGAAGCTCCATAAGCCTCAGAACTTCTGAAAAAAATCCTGCTGCCACACTGATCTCTGAATCTCGGGCATCTAGGAAAAATGATGCACAGTTTAGGAGGACTTCATCTTACAGTGATGCTGGTGTCTCTGAGACATCCTTCATAGATGTGCTCAAGAAACCAGTCATTCCAGAGGCTGACGTAGCCAGCAGGGGTGCAGTGGAGTCATCCGATGGAGGTGGCCAAGTGGGGCGAACTGGCAAGAAGAAAGGCAAGAAAGGAAGACAGATCGATCCTGCTCTCCTTGGCTTTAAGGTATCCAGCAACCGGATCATGATGGGTGAGATCCATCGCCTTGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1640

Amino Acids

184.23

Weight (kDa)

5.61

Isoelectric Point (pI)

62.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 575 - 619 1.4e-10 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 473
Acc36I ACCTGC 4 cut(s) 473, 2286, 2365, 3422
AccB7I CCANNNNNTGG 1 cut(s) 733
AccBSI CCGCTC 1 cut(s) 4344
AccI GTMKAC 1 cut(s) 4478
AccII CGCG 2 cut(s) 708, 4521
AciI CCGC 8 cut(s) 57, 771, 2617, 2860, 2902, 2969, 4344, 4519
AcoI YGGCCR 3 cut(s) 789, 826, 4798
AcsI RAATTY 8 cut(s) 519, 1497, 1555, 2230, 2597, 3614, 3987, 4168
AcyI GRCGYC 1 cut(s) 4516
AfaI GTAC 3 cut(s) 1315, 2102, 4092
AflII CTTAAG 1 cut(s) 1219
AflIII ACRYGT 1 cut(s) 3474
AhdI GACNNNNNGTC 2 cut(s) 655, 4520
AhlI ACTAGT 1 cut(s) 1205
AjnI CCWGG 9 cut(s) 238, 962, 1509, 1746, 1758, 1956, 2289, 3465, 4286
AjuI GAANNNNNNNTTGG 6 cut(s) 2230, 2262, 2922, 2954, 3387, 3419
AloI GAACNNNNNNTCC 2 cut(s) 3033, 3065
Alw21I GWGCWC 3 cut(s) 2334, 3244, 4731
Alw44I GTGCAC 1 cut(s) 3240
AlwNI CAGNNNCTG 3 cut(s) 1406, 2881, 4754
Ama87I CYCGRG 4 cut(s) 306, 1642, 2062, 4641
ApaI GGGCCC 1 cut(s) 584
ApaLI GTGCAC 1 cut(s) 3240
ApoI RAATTY 8 cut(s) 519, 1497, 1555, 2230, 2597, 3614, 3987, 4168
ArsI GACNNNNNNTTYG 2 cut(s) 4000, 4032
Asp700I GAANNNNTTC 1 cut(s) 871
AspLEI GCGC 8 cut(s) 1380, 1821, 2643, 2667, 2730, 2754, 2778, 2928
AsuC2I CCSGG 4 cut(s) 550, 748, 777, 2160
AvaI CYCGRG 4 cut(s) 306, 1642, 2062, 4641
AvaII GGWCC 4 cut(s) 837, 1751, 3434, 3997
AxyI CCTNAGG 1 cut(s) 3582
BaeGI GKGCMC 2 cut(s) 584, 3244
BaeI ACNNNNGTAYC 4 cut(s) 1305, 1338, 2084, 2117
BalI TGGCCA 2 cut(s) 791, 4800
BamHI GGATCC 1 cut(s) 3448
BanII GRGCYC 5 cut(s) 584, 1067, 1284, 2222, 2334
BarI GAAGNNNNNNTAC 2 cut(s) 941, 973
BauI CACGAG 2 cut(s) 2588, 3114
BbsI GAAGAC 5 cut(s) 519, 623, 1523, 3924, 4846
Bbv12I GWGCWC 3 cut(s) 2334, 3244, 4731
BbvCI CCTCAGC 2 cut(s) 90, 2973
BceAI ACGGC 2 cut(s) 408, 4459
BcgI CGANNNNNNTGC 4 cut(s) 2513, 2547, 2978, 3012
BciT130I CCWGG 9 cut(s) 240, 964, 1511, 1748, 1760, 1958, 2291, 3467, 4288
BciVI GTATCC 2 cut(s) 892, 4885
BclI TGATCA 4 cut(s) 1651, 1786, 2392, 3130
BcnI CCSGG 4 cut(s) 550, 748, 777, 2160
BcuI ACTAGT 1 cut(s) 1205
BfaI CTAG 9 cut(s) 444, 498, 1146, 1206, 3206, 3689, 3845, 4356, 4650
BfoI RGCGCY 6 cut(s) 2644, 2668, 2731, 2755, 2779, 2929
BfrI CTTAAG 1 cut(s) 1219
BfuAI ACCTGC 4 cut(s) 473, 2286, 2365, 3422
BfuI GTATCC 2 cut(s) 892, 4885
BglII AGATCT 4 cut(s) 31, 1681, 3685, 4241
BlpI GCTNAGC 2 cut(s) 198, 3894
Bme18I GGWCC 4 cut(s) 837, 1751, 3434, 3997
BmeRI GACNNNNNGTC 2 cut(s) 655, 4520
BmeT110I CYCGRG 4 cut(s) 306, 1642, 2062, 4641
BmiI GGNNCC 9 cut(s) 73, 581, 582, 753, 937, 1752, 3450, 3540, 4285
BoxI GACNNNNGTC 1 cut(s) 3076
BpiI GAAGAC 5 cut(s) 519, 623, 1523, 3924, 4846
BplI GAGNNNNNCTC 2 cut(s) 1028, 1060
BpmI CTGGAG 5 cut(s) 1156, 1296, 2081, 2615, 2888
Bpu10I CCTNAGC 2 cut(s) 90, 2973
Bpu1102I GCTNAGC 2 cut(s) 198, 3894
BpuEI CTTGAG 6 cut(s) 12, 647, 1719, 2065, 2346, 4715
BpuMI CCSGG 4 cut(s) 550, 748, 777, 2160
Bsa29I ATCGAT 1 cut(s) 4848
BsaAI YACGTR 1 cut(s) 3677
BsaBI GATNNNNATC 3 cut(s) 909, 984, 3742
BsaHI GRCGYC 1 cut(s) 4516
BsaI GGTCTC 4 cut(s) 1327, 1906, 2191, 3571
BsaWI WCCGGW 1 cut(s) 4884
BsaXI ACNNNNNCTCC 8 cut(s) 728, 758, 3033, 3063, 4074, 4104, 4440, 4470
Bse21I CCTNAGG 1 cut(s) 3582
Bse3DI GCAATG 1 cut(s) 3924
Bse8I GATNNNNATC 3 cut(s) 909, 984, 3742
BseBI CCWGG 9 cut(s) 240, 964, 1511, 1748, 1760, 1958, 2291, 3467, 4288
BseCI ATCGAT 1 cut(s) 4848
BseJI GATNNNNATC 3 cut(s) 909, 984, 3742
BseMI GCAATG 1 cut(s) 3924
BseRI GAGGAG 9 cut(s) 26, 340, 383, 386, 1968, 2003, 2209, 3041, 4287
BseSI GKGCMC 2 cut(s) 584, 3244
BseYI CCCAGC 1 cut(s) 1454
BsgI GTGCAG 5 cut(s) 1074, 1183, 3841, 4539, 4794
Bsh1236I CGCG 2 cut(s) 708, 4521
BshVI ATCGAT 1 cut(s) 4848
BsiHKAI GWGCWC 3 cut(s) 2334, 3244, 4731
BsiHKCI CYCGRG 4 cut(s) 306, 1642, 2062, 4641
BsiSI CCGG 5 cut(s) 550, 747, 776, 2160, 4885
BslFI GGGAC 3 cut(s) 323, 1764, 3016
BsmBI CGTCTC 1 cut(s) 4507
BsmFI GGGAC 3 cut(s) 323, 1764, 3016
BsmI GAATGC 2 cut(s) 337, 1540
Bso31I GGTCTC 4 cut(s) 1327, 1906, 2191, 3571
BsoBI CYCGRG 4 cut(s) 306, 1642, 2062, 4641
Bsp120I GGGCCC 1 cut(s) 580
Bsp1286I GDGCHC 7 cut(s) 584, 1067, 1284, 2222, 2334, 3244, 4731
Bsp1407I TGTACA 1 cut(s) 4090
Bsp1720I GCTNAGC 2 cut(s) 198, 3894
Bsp68I TCGCGA 1 cut(s) 708
BspACI CCGC 8 cut(s) 57, 771, 2617, 2860, 2902, 2969, 4344, 4519
BspDI ATCGAT 1 cut(s) 4848
BspFNI CGCG 2 cut(s) 708, 4521
BspHI TCATGA 3 cut(s) 3127, 4300, 4890
BspLI GGNNCC 9 cut(s) 73, 581, 582, 753, 937, 1752, 3450, 3540, 4285
BspMAI CTGCAG 3 cut(s) 1251, 2727, 2886
BspMI ACCTGC 4 cut(s) 473, 2286, 2365, 3422
BspTI CTTAAG 1 cut(s) 1219
BspTNI GGTCTC 4 cut(s) 1327, 1906, 2191, 3571
BsrBI CCGCTC 1 cut(s) 4344
BsrDI GCAATG 1 cut(s) 3924
BsrGI TGTACA 1 cut(s) 4090
BssNI GRCGYC 1 cut(s) 4516
BssSI CACGAG 2 cut(s) 2588, 3114
BssT1I CCWWGG 4 cut(s) 75, 3247, 3413, 4861
Bst2BI CACGAG 2 cut(s) 2588, 3114
Bst2UI CCWGG 9 cut(s) 240, 964, 1511, 1748, 1760, 1958, 2291, 3467, 4288
Bst4CI ACNGT 9 cut(s) 356, 767, 1576, 3767, 3965, 4032, 4502, 4668, 4691
Bst6I CTCTTC 2 cut(s) 1101, 1956
BstACI GRCGYC 1 cut(s) 4516
BstAFI CTTAAG 1 cut(s) 1219
BstAPI GCANNNNNTGC 2 cut(s) 1097, 2983
BstAUI TGTACA 1 cut(s) 4090
BstBAI YACGTR 1 cut(s) 3677
BstC8I GCNNGC 9 cut(s) 362, 539, 756, 1589, 3282, 3345, 3821, 4373, 4765
BstDSI CCRYGG 1 cut(s) 1340
BstENI CCTNNNNNAGG 2 cut(s) 3585, 4302
BstFNI CGCG 2 cut(s) 708, 4521
BstH2I RGCGCY 6 cut(s) 2644, 2668, 2731, 2755, 2779, 2929
BstHHI GCGC 8 cut(s) 1380, 1821, 2643, 2667, 2730, 2754, 2778, 2928
BstNI CCWGG 9 cut(s) 240, 964, 1511, 1748, 1760, 1958, 2291, 3467, 4288
BstNSI RCATGY 3 cut(s) 182, 1043, 3478
BstPAI GACNNNNGTC 1 cut(s) 3076
BstSLI GKGCMC 2 cut(s) 584, 3244
BstUI CGCG 2 cut(s) 708, 4521
BstV2I GAAGAC 5 cut(s) 519, 623, 1523, 3924, 4846
BstX2I RGATCY 7 cut(s) 31, 1681, 3448, 3685, 3737, 4241, 4902
BstYI RGATCY 7 cut(s) 31, 1681, 3448, 3685, 3737, 4241, 4902
Bsu15I ATCGAT 1 cut(s) 4848
Bsu36I CCTNAGG 1 cut(s) 3582
BsuI GTATCC 2 cut(s) 892, 4885
BsuTUI ATCGAT 1 cut(s) 4848
BtgI CCRYGG 1 cut(s) 1340
BtgZI GCGATG 3 cut(s) 713, 2516, 4892
BtsI GCAGTG 6 cut(s) 1156, 1244, 2185, 3101, 4396, 4782
BtuMI TCGCGA 1 cut(s) 708
BveI ACCTGC 4 cut(s) 473, 2286, 2365, 3422
Cac8I GCNNGC 9 cut(s) 362, 539, 756, 1589, 3282, 3345, 3821, 4373, 4765
CaiI CAGNNNCTG 3 cut(s) 1406, 2881, 4754
CciI TCATGA 3 cut(s) 3127, 4300, 4890
CfoI GCGC 8 cut(s) 1380, 1821, 2643, 2667, 2730, 2754, 2778, 2928
ClaI ATCGAT 1 cut(s) 4848
CseI GACGC 5 cut(s) 2504, 2876, 4274, 4510, 4524
Csp6I GTAC 3 cut(s) 1314, 2101, 4091
CviQI GTAC 3 cut(s) 1314, 2101, 4091
DraI TTTAAA 2 cut(s) 1356, 4104
DriI GACNNNNNGTC 2 cut(s) 655, 4520
EaeI YGGCCR 3 cut(s) 789, 826, 4798
Eam1104I CTCTTC 2 cut(s) 1101, 1956
Eam1105I GACNNNNNGTC 2 cut(s) 655, 4520
EarI CTCTTC 2 cut(s) 1101, 1956
EciI GGCGGA 2 cut(s) 46, 2958
Ecl136II GAGCTC 1 cut(s) 2332
Eco130I CCWWGG 4 cut(s) 75, 3247, 3413, 4861
Eco147I AGGCCT 1 cut(s) 2167
Eco24I GRGCYC 5 cut(s) 584, 1067, 1284, 2222, 2334
Eco31I GGTCTC 4 cut(s) 1327, 1906, 2191, 3571
Eco32I GATATC 1 cut(s) 982
Eco47I GGWCC 4 cut(s) 837, 1751, 3434, 3997
Eco53kI GAGCTC 1 cut(s) 2332
Eco81I CCTNAGG 1 cut(s) 3582
Eco88I CYCGRG 4 cut(s) 306, 1642, 2062, 4641
EcoICRI GAGCTC 1 cut(s) 2332
EcoNI CCTNNNNNAGG 2 cut(s) 3585, 4302
EcoO109I RGGNCCY 4 cut(s) 580, 581, 1751, 3434
EcoRII CCWGG 9 cut(s) 238, 962, 1509, 1746, 1758, 1956, 2289, 3465, 4286
EcoRV GATATC 1 cut(s) 982
EcoT14I CCWWGG 4 cut(s) 75, 3247, 3413, 4861
EcoT22I ATGCAT 1 cut(s) 2475
EcoT38I GRGCYC 5 cut(s) 584, 1067, 1284, 2222, 2334
ErhI CCWWGG 4 cut(s) 75, 3247, 3413, 4861
Esp3I CGTCTC 1 cut(s) 4507
FalI AAGNNNNNCTT 2 cut(s) 2230, 2262
FaqI GGGAC 3 cut(s) 323, 1764, 3016
FauI CCCGC 1 cut(s) 4337
FbaI TGATCA 4 cut(s) 1651, 1786, 2392, 3130
FblI GTMKAC 1 cut(s) 4478
FriOI GRGCYC 5 cut(s) 584, 1067, 1284, 2222, 2334
FspBI CTAG 9 cut(s) 444, 498, 1146, 1206, 3206, 3689, 3845, 4356, 4650
GlaI GCGC 8 cut(s) 1379, 1820, 2642, 2666, 2729, 2753, 2777, 2927
GsaI CCCAGC 1 cut(s) 1458
GsuI CTGGAG 5 cut(s) 1156, 1296, 2081, 2615, 2888
HaeII RGCGCY 6 cut(s) 2644, 2668, 2731, 2755, 2779, 2929
HapII CCGG 5 cut(s) 550, 747, 776, 2160, 4885
HgaI GACGC 5 cut(s) 2504, 2876, 4274, 4510, 4524
HhaI GCGC 8 cut(s) 1380, 1821, 2643, 2667, 2730, 2754, 2778, 2928
Hin1I GRCGYC 1 cut(s) 4516
Hin6I GCGC 8 cut(s) 1378, 1819, 2641, 2665, 2728, 2752, 2776, 2926
HinP1I GCGC 8 cut(s) 1378, 1819, 2641, 2665, 2728, 2752, 2776, 2926
HincII GTYRAC 2 cut(s) 352, 649
HindII GTYRAC 2 cut(s) 352, 649
HindIII AAGCTT 2 cut(s) 2364, 3817
HpaI GTTAAC 1 cut(s) 352
HpaII CCGG 5 cut(s) 550, 747, 776, 2160, 4885
Hpy166II GTNNAC 6 cut(s) 352, 649, 1610, 3242, 3680, 4479
Hpy8I GTNNAC 6 cut(s) 352, 649, 1610, 3242, 3680, 4479
Hpy99I CGWCG 3 cut(s) 360, 3771, 3909
HpyCH4III ACNGT 9 cut(s) 356, 767, 1576, 3767, 3965, 4032, 4502, 4668, 4691
HpyCH4IV ACGT 4 cut(s) 691, 2130, 3676, 4758
HpySE526I ACGT 4 cut(s) 691, 2130, 3676, 4758
Hsp92I GRCGYC 1 cut(s) 4516
HspAI GCGC 8 cut(s) 1378, 1819, 2641, 2665, 2728, 2752, 2776, 2926
Ksp22I TGATCA 4 cut(s) 1651, 1786, 2392, 3130
KspAI GTTAAC 1 cut(s) 352
MaeI CTAG 9 cut(s) 444, 498, 1146, 1206, 3206, 3689, 3845, 4356, 4650
MaeII ACGT 4 cut(s) 691, 2130, 3676, 4758
MbiI CCGCTC 1 cut(s) 4344
MfeI CAATTG 4 cut(s) 2621, 2930, 2957, 3044
MflI RGATCY 7 cut(s) 31, 1681, 3448, 3685, 3737, 4241, 4902
MhlI GDGCHC 7 cut(s) 584, 1067, 1284, 2222, 2334, 3244, 4731
MlsI TGGCCA 2 cut(s) 791, 4800
MluNI TGGCCA 2 cut(s) 791, 4800
MlyI GAGTC 6 cut(s) 441, 596, 2000, 3568, 4559, 4790
Mox20I TGGCCA 2 cut(s) 791, 4800
Mph1103I ATGCAT 1 cut(s) 2475
MroXI GAANNNNTTC 1 cut(s) 871
MscI TGGCCA 2 cut(s) 791, 4800
MslI CAYNNNNRTG 4 cut(s) 169, 574, 3888, 4724
Msp20I TGGCCA 2 cut(s) 791, 4800
MspA1I CMGCKG 6 cut(s) 2207, 2617, 2722, 2860, 2881, 2902
MspCI CTTAAG 1 cut(s) 1219
MspI CCGG 5 cut(s) 550, 747, 776, 2160, 4885
MunI CAATTG 4 cut(s) 2621, 2930, 2957, 3044
Mva1269I GAATGC 2 cut(s) 337, 1540
MvaI CCWGG 9 cut(s) 240, 964, 1511, 1748, 1760, 1958, 2291, 3467, 4288
MvnI CGCG 2 cut(s) 708, 4521
NciI CCSGG 4 cut(s) 550, 748, 777, 2160
NlaIV GGNNCC 9 cut(s) 73, 581, 582, 753, 937, 1752, 3450, 3540, 4285
NmeAIII GCCGAG 1 cut(s) 854
NmuCI GTSAC 5 cut(s) 111, 162, 433, 1393, 2273
NruI TCGCGA 1 cut(s) 708
NsiI ATGCAT 1 cut(s) 2475
NspI RCATGY 3 cut(s) 182, 1043, 3478
PaeR7I CTCGAG 2 cut(s) 1642, 2062
PagI TCATGA 3 cut(s) 3127, 4300, 4890
PaqCI CACCTGC 1 cut(s) 473
PasI CCCWGGG 1 cut(s) 963
PceI AGGCCT 1 cut(s) 2167
PciI ACATGT 1 cut(s) 3474
PctI GAATGC 2 cut(s) 337, 1540
PdmI GAANNNNTTC 1 cut(s) 871
PflMI CCANNNNNTGG 1 cut(s) 733
PfoI TCCNGGA 3 cut(s) 548, 1956, 2158
PleI GAGTC 6 cut(s) 440, 596, 1999, 3567, 4558, 4789
PpsI GAGTC 6 cut(s) 440, 596, 1999, 3567, 4558, 4789
Ppu21I YACGTR 1 cut(s) 3677
PpuMI RGGWCCY 2 cut(s) 1751, 3434
PscI ACATGT 1 cut(s) 3474
PshAI GACNNNNGTC 1 cut(s) 3076
Psp124BI GAGCTC 1 cut(s) 2334
Psp5II RGGWCCY 2 cut(s) 1751, 3434
Psp6I CCWGG 9 cut(s) 238, 962, 1509, 1746, 1758, 1956, 2289, 3465, 4286
PspFI CCCAGC 1 cut(s) 1454
PspGI CCWGG 9 cut(s) 238, 962, 1509, 1746, 1758, 1956, 2289, 3465, 4286
PspN4I GGNNCC 9 cut(s) 73, 581, 582, 753, 937, 1752, 3450, 3540, 4285
PspOMI GGGCCC 1 cut(s) 580
PspPPI RGGWCCY 2 cut(s) 1751, 3434
PspXI VCTCGAGB 1 cut(s) 1642
PstI CTGCAG 3 cut(s) 1251, 2727, 2886
PstNI CAGNNNCTG 3 cut(s) 1406, 2881, 4754
PsuI RGATCY 7 cut(s) 31, 1681, 3448, 3685, 3737, 4241, 4902
PvuII CAGCTG 3 cut(s) 2207, 2722, 2881
RruI TCGCGA 1 cut(s) 708
RsaI GTAC 3 cut(s) 1315, 2102, 4092
RsaNI GTAC 3 cut(s) 1314, 2101, 4091
RseI CAYNNNNRTG 4 cut(s) 169, 574, 3888, 4724
SacI GAGCTC 1 cut(s) 2334
SchI GAGTC 6 cut(s) 441, 596, 2000, 3568, 4559, 4790
SduI GDGCHC 7 cut(s) 584, 1067, 1284, 2222, 2334, 3244, 4731
Sfr274I CTCGAG 2 cut(s) 1642, 2062
SinI GGWCC 4 cut(s) 837, 1751, 3434, 3997
SlaI CTCGAG 2 cut(s) 1642, 2062
SmiMI CAYNNNNRTG 4 cut(s) 169, 574, 3888, 4724
SmlI CTYRAG 9 cut(s) 27, 626, 1219, 1642, 1734, 2044, 2062, 2361, 4730
SmoI CTYRAG 9 cut(s) 27, 626, 1219, 1642, 1734, 2044, 2062, 2361, 4730
SpeI ACTAGT 1 cut(s) 1205
SseBI AGGCCT 1 cut(s) 2167
SsiI CCGC 8 cut(s) 57, 771, 2617, 2860, 2902, 2969, 4344, 4519
SspMI CTAG 9 cut(s) 444, 498, 1146, 1206, 3206, 3689, 3845, 4356, 4650
SstI GAGCTC 1 cut(s) 2334
StuI AGGCCT 1 cut(s) 2167
StyI CCWWGG 4 cut(s) 75, 3247, 3413, 4861
TaaI ACNGT 9 cut(s) 356, 767, 1576, 3767, 3965, 4032, 4502, 4668, 4691
TaiI ACGT 4 cut(s) 694, 2133, 3679, 4761
TatI WGTACW 1 cut(s) 4090
TauI GCSGC 4 cut(s) 773, 2620, 2905, 4521
TseFI GTSAC 5 cut(s) 111, 162, 433, 1393, 2273
Tsp45I GTSAC 5 cut(s) 111, 162, 433, 1393, 2273
TspGWI ACGGA 3 cut(s) 24, 1357, 3929
Van91I CCANNNNNTGG 1 cut(s) 733
Vha464I CTTAAG 1 cut(s) 1219
VneI GTGCAC 1 cut(s) 3240
VpaK11BI GGWCC 4 cut(s) 837, 1751, 3434, 3997
XagI CCTNNNNNAGG 2 cut(s) 3585, 4302
XapI RAATTY 8 cut(s) 519, 1497, 1555, 2230, 2597, 3614, 3987, 4168
XbaI TCTAGA 1 cut(s) 3688
XceI RCATGY 3 cut(s) 182, 1043, 3478
XcmI CCANNNNNNNNNTGG 2 cut(s) 799, 4258
XhoI CTCGAG 2 cut(s) 1642, 2062
XmiI GTMKAC 1 cut(s) 4478
XmnI GAANNNNTTC 1 cut(s) 871
XspI CTAG 9 cut(s) 444, 498, 1146, 1206, 3206, 3689, 3845, 4356, 4650
Zsp2I ATGCAT 1 cut(s) 2475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.