FvH4_7g01770

F-box WD-40 repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004801
Physical Location & Seq
Forward (+)
2159768 .. 2165824
6057 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g01770.t12

Sequence Viewer

Length: 930 bp
ATGGGTTTGCTTCTTACTGGCATTGGTGATAAGGTTATGCGCCTTTGGTCATTGGAAAGCTATAAATGTGTGGGAGAATATCCCATTCCAGACAAGGTCCCTTTAGTTGACTTTGATTTTGATGAAAGCAAGATTGTTGGTTTGGTCGGCAGTCGTATATGCATATGGAGGCGGAATGGGATAAGAAGTATATTTCCCTCACGTGAGGGCACCTTTCCAAAGGGCTTATGCATGCGTTACTGTGATCCTGATGCTGTGGTTGGATGTGTTGACGGGACAGCTCGAATTTTTGACATGTACAGTCAACGATGTTCCCAAATTGTGAAAATGCATGCCGGTCCAGTGACGTGCTTATGTTTGAGTGAGGATCAATTGATACTTAGCGGTTCTTCTCTTGGCAGTATCAGTGTGTACGGAGTTTTATCTGATCAGCGGGTTGCAGAATTAAGATCGACACATTCCACAGGCATAAGGACTTTGTGTTTCAACCCCTCATCACACCTGCTGTTCGCTGGATCAACTTCTGGATACGTAGATTGTTGGGACATTAGGACATGGAGAAGTTTGTGGGAGACACGGGTGAGTCCAAATGTGGTATATTCAATGCAACACCTGAGAAATGATACGTCAAGCTTGGTTGTTGGTGGAATAGATGGTGTGCTACGTATACTGAATCAGAACACAGGTGAAGTAGTTTCAAGGTTTGTTTTGGAAGGCAACTTATTATCAAGTTCAAGTTCAAGTTCTCATCAACACGTAGTTGGAAGTGGAACTGTCCAAAGAATGATAGGACGGAGGCTTTCACAAAATGCTTCAATTGACAGCATTCCTAGAAGCAGTCGACCTCCCATCACTTGCTTGGCGGTCTGGATGAAGAAGGTTATCACCACACACAATAATAAGTACATCAGAACGTGGAAGTTCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
F-box F-box-like WD40

Protein Analysis

310

Amino Acids

34.31

Weight (kDa)

9.08

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_HPS5 PF23756 26 - 143 7.4e-06 HPS5 beta-propeller
Beta-prop_EML_2 PF23414 28 - 134 1.7e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_ACSF4 PF13570 29 - 210 2.2e-12 Beta-alanine-activating enzyme, beta-propeller
WD40_WDHD1_1st PF24817 29 - 246 1.3e-11 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 38 - 176 3.3e-16 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 39 - 179 7.5e-11 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 39 - 209 9.5e-14 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 40 - 171 1.1e-11 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 40 - 126 6.2e-06 WDR36/Utp21 first beta-propeller
Beta-prop_THOC3 PF25174 41 - 210 4.1e-13 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 42 - 194 5.1e-16 WDR5 beta-propeller domain
Beta-prop_EML PF23409 68 - 168 1.3e-06 Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19 PF24814 78 - 188 1.2e-06 Prp19 WD40 domain
WDR55 PF24796 79 - 259 4.2e-09 WDR55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 510
Acc36I ACCTGC 1 cut(s) 510
AccB1I GGYRCC 1 cut(s) 209
AccI GTMKAC 2 cut(s) 667, 841
AciI CCGC 4 cut(s) 172, 384, 433, 863
AclWI GGATC 3 cut(s) 239, 375, 523
AcsI RAATTY 1 cut(s) 285
AcvI CACGTG 1 cut(s) 203
AfaI GTAC 3 cut(s) 299, 413, 905
AflIII ACRYGT 2 cut(s) 294, 754
AgsI TTSAA 7 cut(s) 487, 603, 699, 735, 741, 816, 925
AjiI CACGTC 1 cut(s) 348
AluBI AGCT 3 cut(s) 60, 281, 633
AluI AGCT 3 cut(s) 60, 281, 633
Alw26I GTCTC 1 cut(s) 566
AlwI GGATC 3 cut(s) 239, 375, 523
ApoI RAATTY 1 cut(s) 285
ArsI GACNNNNNNTTYG 2 cut(s) 101, 133
AspLEI GCGC 1 cut(s) 42
AspS9I GGNCC 2 cut(s) 97, 338
AsuHPI GGTGA 4 cut(s) 38, 592, 698, 877
AvaII GGWCC 2 cut(s) 97, 338
BaeGI GKGCMC 1 cut(s) 212
BanI GGYRCC 1 cut(s) 209
BbrPI CACGTG 1 cut(s) 203
BccI CCATC 2 cut(s) 647, 857
BciVI GTATCC 1 cut(s) 521
BclI TGATCA 1 cut(s) 427
BcoDI GTCTC 1 cut(s) 566
BfaI CTAG 1 cut(s) 831
BfuAI ACCTGC 1 cut(s) 510
BfuI GTATCC 1 cut(s) 521
Bme18I GGWCC 2 cut(s) 97, 338
BmgBI CACGTC 1 cut(s) 348
BmgT120I GGNCC 2 cut(s) 97, 338
BmiI GGNNCC 2 cut(s) 99, 211
BmsI GCATC 1 cut(s) 241
BsaAI YACGTR 4 cut(s) 203, 532, 665, 757
Bse118I RCCGGY 1 cut(s) 335
Bse1I ACTGG 2 cut(s) 22, 341
BseGI GGATG 2 cut(s) 269, 876
BseMII CTCAG 1 cut(s) 605
BseNI ACTGG 2 cut(s) 22, 341
BseSI GKGCMC 1 cut(s) 212
BshNI GGYRCC 1 cut(s) 209
BsiSI CCGG 1 cut(s) 336
BslFI GGGAC 3 cut(s) 83, 289, 557
BsmAI GTCTC 1 cut(s) 566
BsmFI GGGAC 3 cut(s) 83, 289, 557
BsmI GAATGC 1 cut(s) 825
Bsp1286I GDGCHC 1 cut(s) 212
Bsp1407I TGTACA 1 cut(s) 297
Bsp143I GATC 5 cut(s) 244, 367, 427, 449, 515
BspACI CCGC 4 cut(s) 172, 384, 433, 863
BspCNI CTCAG 1 cut(s) 606
BspLI GGNNCC 2 cut(s) 99, 211
BspMI ACCTGC 1 cut(s) 510
BspPI GGATC 3 cut(s) 239, 375, 523
BspT107I GGYRCC 1 cut(s) 209
BsrFI RCCGGY 1 cut(s) 335
BsrGI TGTACA 1 cut(s) 297
BsrI ACTGG 2 cut(s) 22, 341
BssAI RCCGGY 1 cut(s) 335
BssMI GATC 5 cut(s) 244, 367, 427, 449, 515
BssNAI GTATAC 1 cut(s) 668
Bst1107I GTATAC 1 cut(s) 668
Bst4CI ACNGT 3 cut(s) 242, 302, 775
BstAUI TGTACA 1 cut(s) 297
BstBAI YACGTR 4 cut(s) 203, 532, 665, 757
BstC8I GCNNGC 2 cut(s) 233, 333
BstDEI CTNAG 2 cut(s) 380, 614
BstF5I GGATG 2 cut(s) 269, 876
BstHHI GCGC 1 cut(s) 42
BstKTI GATC 5 cut(s) 247, 370, 430, 452, 518
BstMAI GTCTC 1 cut(s) 566
BstMBI GATC 5 cut(s) 244, 367, 427, 449, 515
BstNSI RCATGY 3 cut(s) 235, 298, 335
BstSLI GKGCMC 1 cut(s) 212
BstSNI TACGTA 2 cut(s) 532, 665
BstZ17I GTATAC 1 cut(s) 668
BsuI GTATCC 1 cut(s) 521
BtrI CACGTC 1 cut(s) 348
BtsCI GGATG 2 cut(s) 269, 876
BtsIMutI CAGTG 2 cut(s) 348, 412
BveI ACCTGC 1 cut(s) 510
Cac8I GCNNGC 2 cut(s) 233, 333
CfoI GCGC 1 cut(s) 42
Cfr10I RCCGGY 1 cut(s) 335
Cfr13I GGNCC 2 cut(s) 97, 338
Csp6I GTAC 3 cut(s) 298, 412, 904
CviAII CATG 4 cut(s) 232, 295, 332, 555
CviJI RGCY 5 cut(s) 60, 225, 281, 633, 799
CviKI_1 RGCY 5 cut(s) 60, 225, 281, 633, 799
CviQI GTAC 3 cut(s) 298, 412, 904
DdeI CTNAG 2 cut(s) 380, 614
DpnI GATC 5 cut(s) 246, 369, 429, 451, 517
DpnII GATC 5 cut(s) 244, 367, 427, 449, 515
EciI GGCGGA 1 cut(s) 187
Eco105I TACGTA 2 cut(s) 532, 665
Eco47I GGWCC 2 cut(s) 97, 338
Eco72I CACGTG 1 cut(s) 203
EcoO109I RGGNCCY 1 cut(s) 97
EcoT22I ATGCAT 3 cut(s) 164, 233, 333
FaeI CATG 4 cut(s) 235, 298, 335, 558
FaqI GGGAC 3 cut(s) 83, 289, 557
FatI CATG 4 cut(s) 231, 294, 331, 554
FauI CCCGC 1 cut(s) 426
FauNDI CATATG 1 cut(s) 164
FbaI TGATCA 1 cut(s) 427
FblI GTMKAC 2 cut(s) 667, 841
FokI GGATG 2 cut(s) 276, 883
FspBI CTAG 1 cut(s) 831
GlaI GCGC 1 cut(s) 41
HapII CCGG 1 cut(s) 336
HhaI GCGC 1 cut(s) 42
Hin1II CATG 4 cut(s) 235, 298, 335, 558
Hin6I GCGC 1 cut(s) 40
HinP1I GCGC 1 cut(s) 40
HincII GTYRAC 4 cut(s) 109, 271, 305, 842
HindII GTYRAC 4 cut(s) 109, 271, 305, 842
HindIII AAGCTT 1 cut(s) 631
HinfI GANTC 2 cut(s) 583, 673
HpaII CCGG 1 cut(s) 336
HphI GGTGA 4 cut(s) 38, 592, 698, 877
Hpy166II GTNNAC 6 cut(s) 109, 271, 305, 412, 668, 842
Hpy188I TCNGA 3 cut(s) 427, 678, 911
Hpy188III TCNNGA 4 cut(s) 89, 248, 525, 868
Hpy8I GTNNAC 6 cut(s) 109, 271, 305, 412, 668, 842
HpyAV CCTTC 2 cut(s) 707, 871
HpyCH4III ACNGT 3 cut(s) 242, 302, 775
HpyCH4IV ACGT 7 cut(s) 202, 347, 531, 626, 664, 756, 914
HpyCH4V TGCA 5 cut(s) 162, 231, 331, 440, 607
HpyF3I CTNAG 2 cut(s) 380, 614
HpySE526I ACGT 7 cut(s) 202, 347, 531, 626, 664, 756, 914
Hsp92II CATG 4 cut(s) 235, 298, 335, 558
HspAI GCGC 1 cut(s) 40
Ksp22I TGATCA 1 cut(s) 427
Kzo9I GATC 5 cut(s) 244, 367, 427, 449, 515
LweI GCATC 1 cut(s) 241
MaeI CTAG 1 cut(s) 831
MaeII ACGT 7 cut(s) 202, 347, 531, 626, 664, 756, 914
MaeIII GTNAC 2 cut(s) 236, 343
MalI GATC 5 cut(s) 246, 369, 429, 451, 517
MboI GATC 5 cut(s) 244, 367, 427, 449, 515
MboII GAAGA 2 cut(s) 381, 886
MfeI CAATTG 2 cut(s) 371, 816
MhlI GDGCHC 1 cut(s) 212
MluCI AATT 5 cut(s) 285, 318, 371, 443, 816
MlyI GAGTC 1 cut(s) 592
MmeI TCCRAC 2 cut(s) 241, 742
MnlI CCTC 7 cut(s) 162, 199, 208, 358, 502, 789, 855
Mph1103I ATGCAT 3 cut(s) 164, 233, 333
MseI TTAA 1 cut(s) 446
MspA1I CMGCKG 1 cut(s) 433
MspI CCGG 1 cut(s) 336
MunI CAATTG 2 cut(s) 371, 816
Mva1269I GAATGC 1 cut(s) 825
NdeI CATATG 1 cut(s) 164
NdeII GATC 5 cut(s) 244, 367, 427, 449, 515
NlaIII CATG 4 cut(s) 235, 298, 335, 558
NlaIV GGNNCC 2 cut(s) 99, 211
NmuCI GTSAC 1 cut(s) 343
NsiI ATGCAT 3 cut(s) 164, 233, 333
NspI RCATGY 3 cut(s) 235, 298, 335
PaeI GCATGC 2 cut(s) 235, 335
PaqCI CACCTGC 1 cut(s) 510
PciI ACATGT 1 cut(s) 294
PctI GAATGC 1 cut(s) 825
PfeI GAWTC 1 cut(s) 673
PflFI GACNNNGTC 1 cut(s) 95
PleI GAGTC 1 cut(s) 591
PmaCI CACGTG 1 cut(s) 203
PmlI CACGTG 1 cut(s) 203
PpsI GAGTC 1 cut(s) 591
Ppu21I YACGTR 4 cut(s) 203, 532, 665, 757
PpuMI RGGWCCY 1 cut(s) 97
PscI ACATGT 1 cut(s) 294
Psp5II RGGWCCY 1 cut(s) 97
PspCI CACGTG 1 cut(s) 203
PspN4I GGNNCC 2 cut(s) 99, 211
PspPI GGNCC 2 cut(s) 97, 338
PspPPI RGGWCCY 1 cut(s) 97
PsyI GACNNNGTC 1 cut(s) 95
RsaI GTAC 3 cut(s) 299, 413, 905
RsaNI GTAC 3 cut(s) 298, 412, 904
SalI GTCGAC 1 cut(s) 840
SaqAI TTAA 1 cut(s) 446
Sau3AI GATC 5 cut(s) 244, 367, 427, 449, 515
Sau96I GGNCC 2 cut(s) 97, 338
SchI GAGTC 1 cut(s) 592
SduI GDGCHC 1 cut(s) 212
SfaNI GCATC 1 cut(s) 241
SinI GGWCC 2 cut(s) 97, 338
SnaBI TACGTA 2 cut(s) 532, 665
SphI GCATGC 2 cut(s) 235, 335
Sse9I AATT 5 cut(s) 285, 318, 371, 443, 816
SsiI CCGC 4 cut(s) 172, 384, 433, 863
SspMI CTAG 1 cut(s) 831
TaaI ACNGT 3 cut(s) 242, 302, 775
TaiI ACGT 7 cut(s) 205, 350, 534, 629, 667, 759, 917
TaqI TCGA 3 cut(s) 283, 452, 841
TasI AATT 5 cut(s) 285, 318, 371, 443, 816
TatI WGTACW 2 cut(s) 297, 903
TfiI GAWTC 1 cut(s) 673
Tru1I TTAA 1 cut(s) 446
Tru9I TTAA 1 cut(s) 446
TscAI CASTG 2 cut(s) 348, 412
TseFI GTSAC 1 cut(s) 343
Tsp45I GTSAC 1 cut(s) 343
TspDTI ATGAA 2 cut(s) 138, 887
TspGWI ACGGA 2 cut(s) 429, 808
TspRI CASTG 2 cut(s) 348, 412
Tth111I GACNNNGTC 1 cut(s) 95
VpaK11BI GGWCC 2 cut(s) 97, 338
XapI RAATTY 1 cut(s) 285
XceI RCATGY 3 cut(s) 235, 298, 335
XcmI CCANNNNNNNNNTGG 1 cut(s) 856
XmiI GTMKAC 2 cut(s) 667, 841
XspI CTAG 1 cut(s) 831
Zsp2I ATGCAT 3 cut(s) 164, 233, 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.