MD07G1023200.v1.1

F-box WD-40 repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004801
Physical Location & Seq
Forward (+)
1937201 .. 1943411
6211 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1023200.v1.1.491

Sequence Viewer

Length: 1299 bp
ATGGATATCTCTCTCACCGGCGACAAGAAGAGGCGGAGCAGCAGCTCAAGAGCAAGATCTCCGACCACAATACTCTCTCTCCAACACGAAATCCTTTGCACCATCTTCTCTTTCCTCGACTTCTTCGACCTCGCAAGTTGTTCCGCCGTATGCAAAACCTGGAGCACGGTGATCAACAAGTCTAAGTTGCTCCGAGTGTTTTGCCGCAAGCAATGGCAGATGGGTCTTGCGGGTTCTAGCAATGGGTCTAGTGATGGTCCTGAGAAACCTTGGGAGGTTATTTTGGAGGAGATGGCTGTGGAGCGTCATAAACTGGCGTTACAAGATGGTCGAATTCGAGTCGATCACTTGAGGGGTCACTCTGTTGGGGTTGGACAGTGTCGAAAGAAGATGGGGTTGCTTCTCACTGGCGGCGGTGATAAGGTTATGCGTCTTTGGTCATTGGAAAGCTATAAATGTGTGGGAGAATATCCCACTCCAGACAAGGTCCATTTGGTTGACTTTGATTTTGATGAAAGCAAGATTGTAGGTTTGGTTGGCACTCGTATATGCATATGGAGGAGGAATGCGATGAGAAGTATATTTCCCTCATGTGAGGGCACCTTTCCGAAGGGTTTATGCATGCGTTACTGCGATCCTGAGGCTGTGGTTGGATGCGAGGATGGGACAGCTCGTGTATTTGACATGTATAGTAAACAATGTTCTCAAATCATTAGAACGCATGCGGGGCCAGTAACATGCTTATGTTTGAGTGATGATCAGTTGATACTTAGTGGTTCATCTCTTGGTGGTATCACAATATCTGGACTTTCATCTGGCGAGCAGGTTGCGAAATTAAGTTCAAATGATTCCTCAGGCATAAAGACTTTGTGTTTCAACCCAGGCTCTCACTTAGTATTTGCTGGATCGGCTGCTGGATATGCATTATGTTGGGACGTTAGGACAATGAAATGCTTATGGGAGACACGAGTGAGTCCAAATAGGGTATACACGTTGCAGCACATGAGAAATGATGCATCGACGTTGGCGGTTGGTGGAATAGACGGTGTGCTGCGTCTTCTGAACCAGTGCACAGGTGAAGTAATTTCAAGATGCGTCTTGGATGGATATTTATCGTCAAGTTCTCAAAGCACGATTGGGGCTACCCAAAGAATGAGAGGAACAAGGCTTTCGGAAGATACCCCCGTTGAGAGCATTCTTAGTACCAGACGGCCTCCCATTACGTGTTTGATTGTCGGGATGAGGAAGGTTATCACCACGCATTACGATAAGTACATCAGAATCTGGAAATTCGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
F-box F-box-like WD40

Protein Analysis

433

Amino Acids

47.73

Weight (kDa)

8.89

Isoelectric Point (pI)

46.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 24 - 64 3.9e-09 F-box domain
F-box-like PF12937 29 - 69 9.8e-10 F-box-like
Beta-prop_WDR36-Utp21_1st PF25171 176 - 365 1.3e-06 WDR36/Utp21 first beta-propeller
Beta-prop_EML_2 PF23414 205 - 305 5.5e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 206 - 326 1.7e-08 WDHD1 first WD40 domain
Beta-prop_ACSF4 PF13570 206 - 369 6.9e-08 Beta-alanine-activating enzyme, beta-propeller
Beta-prop_WDR3_1st PF25173 215 - 353 1.8e-13 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 215 - 350 6.4e-10 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 216 - 364 7.2e-11 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 217 - 355 1.6e-11 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 221 - 365 9.4e-12 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 241 - 357 6.9e-08 WDR3 second beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 814
AccB1I GGYRCC 1 cut(s) 599
AccI GTMKAC 1 cut(s) 987
AciI CCGC 8 cut(s) 34, 144, 205, 230, 411, 414, 725, 1028
AclWI GGATC 2 cut(s) 629, 913
AcsI RAATTY 2 cut(s) 333, 1289
AfaI GTAC 2 cut(s) 1204, 1274
AflIII ACRYGT 3 cut(s) 684, 990, 1223
AgsI TTSAA 3 cut(s) 843, 877, 1089
AjnI CCWGG 2 cut(s) 158, 880
AluBI AGCT 3 cut(s) 45, 450, 671
AluI AGCT 3 cut(s) 45, 450, 671
Alw21I GWGCWC 2 cut(s) 167, 1073
Alw26I GTCTC 1 cut(s) 956
Alw44I GTGCAC 1 cut(s) 1069
AlwI GGATC 2 cut(s) 629, 913
AlwNI CAGNNNCTG 1 cut(s) 1284
AoxI GGCC 2 cut(s) 728, 1211
ApaLI GTGCAC 1 cut(s) 1069
ApeKI GCWGC 5 cut(s) 39, 42, 911, 997, 1051
ApoI RAATTY 2 cut(s) 333, 1289
ArsI GACNNNNNNTTYG 2 cut(s) 491, 523
AspS9I GGNCC 3 cut(s) 257, 487, 728
AsuHPI GGTGA 5 cut(s) 7, 181, 428, 1088, 1246
AvaII GGWCC 2 cut(s) 257, 487
AxyI CCTNAGG 2 cut(s) 639, 853
BaeGI GKGCMC 2 cut(s) 602, 1073
BaeI ACNNNNGTAYC 2 cut(s) 1170, 1203
BanI GGYRCC 1 cut(s) 599
BauI CACGAG 2 cut(s) 672, 966
BbsI GAAGAC 1 cut(s) 1049
Bbv12I GWGCWC 2 cut(s) 167, 1073
BbvI GCAGC 5 cut(s) 51, 54, 898, 1009, 1038
BccI CCATC 8 cut(s) 110, 214, 248, 286, 320, 385, 656, 1097
BceAI ACGGC 2 cut(s) 131, 1226
BcgI CGANNNNNNTGC 4 cut(s) 183, 217, 809, 843
BciT130I CCWGG 2 cut(s) 160, 882
BclI TGATCA 2 cut(s) 171, 757
BcoDI GTCTC 1 cut(s) 956
BfaI CTAG 2 cut(s) 237, 249
BfuAI ACCTGC 1 cut(s) 814
BglII AGATCT 1 cut(s) 56
BisI GCNGC 7 cut(s) 40, 43, 205, 412, 912, 998, 1052
BlsI GCNGC 7 cut(s) 41, 44, 206, 413, 913, 999, 1053
Bme1390I CCNGG 2 cut(s) 160, 882
Bme18I GGWCC 2 cut(s) 257, 487
BmgT120I GGNCC 3 cut(s) 257, 487, 728
BmiI GGNNCC 2 cut(s) 601, 729
BmrFI CCNGG 2 cut(s) 160, 882
BmsI GCATC 4 cut(s) 644, 1003, 1025, 1082
BpiI GAAGAC 1 cut(s) 1049
BpmI CTGGAG 2 cut(s) 181, 462
BpuEI CTTGAG 2 cut(s) 31, 370
BsaAI YACGTR 1 cut(s) 1224
BsaBI GATNNNNATC 1 cut(s) 1111
BsaJI CCNNGG 2 cut(s) 269, 880
BsaXI ACNNNNNCTCC 2 cut(s) 63, 93
Bse118I RCCGGY 1 cut(s) 17
Bse1I ACTGG 4 cut(s) 318, 412, 731, 1066
Bse21I CCTNAGG 2 cut(s) 639, 853
Bse3DI GCAATG 2 cut(s) 218, 247
Bse8I GATNNNNATC 1 cut(s) 1111
BseBI CCWGG 2 cut(s) 160, 882
BseDI CCNNGG 2 cut(s) 269, 880
BseGI GGATG 4 cut(s) 659, 667, 1108, 1245
BseJI GATNNNNATC 1 cut(s) 1111
BseMI GCAATG 2 cut(s) 218, 247
BseMII CTCAG 3 cut(s) 252, 630, 867
BseNI ACTGG 4 cut(s) 318, 412, 731, 1066
BseRI GAGGAG 2 cut(s) 302, 574
BseSI GKGCMC 2 cut(s) 602, 1073
BseXI GCAGC 5 cut(s) 51, 54, 898, 1009, 1038
BshFI GGCC 2 cut(s) 730, 1213
BshNI GGYRCC 1 cut(s) 599
BsiHKAI GWGCWC 2 cut(s) 167, 1073
BsiSI CCGG 1 cut(s) 18
BslFI GGGAC 2 cut(s) 679, 947
BsmAI GTCTC 1 cut(s) 956
BsmFI GGGAC 2 cut(s) 679, 947
BsmI GAATGC 2 cut(s) 571, 1194
BsnI GGCC 2 cut(s) 730, 1213
Bsp1286I GDGCHC 3 cut(s) 167, 602, 1073
Bsp143I GATC 6 cut(s) 56, 171, 343, 634, 757, 905
BspACI CCGC 8 cut(s) 34, 144, 205, 230, 411, 414, 725, 1028
BspANI GGCC 2 cut(s) 730, 1213
BspCNI CTCAG 3 cut(s) 253, 631, 866
BspLI GGNNCC 2 cut(s) 601, 729
BspMI ACCTGC 1 cut(s) 814
BspPI GGATC 2 cut(s) 629, 913
BspT107I GGYRCC 1 cut(s) 599
BsrDI GCAATG 2 cut(s) 218, 247
BsrFI RCCGGY 1 cut(s) 17
BsrI ACTGG 4 cut(s) 318, 412, 731, 1066
BssAI RCCGGY 1 cut(s) 17
BssECI CCNNGG 2 cut(s) 269, 880
BssMI GATC 6 cut(s) 56, 171, 343, 634, 757, 905
BssNAI GTATAC 1 cut(s) 988
BssSI CACGAG 2 cut(s) 672, 966
BssT1I CCWWGG 1 cut(s) 269
Bst1107I GTATAC 1 cut(s) 988
Bst2BI CACGAG 2 cut(s) 672, 966
Bst2UI CCWGG 2 cut(s) 160, 882
Bst4CI ACNGT 3 cut(s) 169, 378, 1046
Bst6I CTCTTC 1 cut(s) 23
BstBAI YACGTR 1 cut(s) 1224
BstC8I GCNNGC 4 cut(s) 209, 623, 723, 821
BstDEI CTNAG 7 cut(s) 183, 261, 639, 770, 853, 892, 1199
BstF5I GGATG 4 cut(s) 659, 667, 1108, 1245
BstKTI GATC 6 cut(s) 59, 174, 346, 637, 760, 908
BstMAI GTCTC 1 cut(s) 956
BstMBI GATC 6 cut(s) 56, 171, 343, 634, 757, 905
BstMWI GCNNNNNNNGC 3 cut(s) 727, 908, 920
BstNI CCWGG 2 cut(s) 160, 882
BstNSI RCATGY 4 cut(s) 625, 688, 725, 741
BstSCI CCNGG 2 cut(s) 158, 880
BstSLI GKGCMC 2 cut(s) 602, 1073
BstV1I GCAGC 5 cut(s) 51, 54, 898, 1009, 1038
BstV2I GAAGAC 1 cut(s) 1049
BstX2I RGATCY 1 cut(s) 56
BstYI RGATCY 1 cut(s) 56
BstZ17I GTATAC 1 cut(s) 988
Bsu36I CCTNAGG 2 cut(s) 639, 853
BsuRI GGCC 2 cut(s) 730, 1213
BtgZI GCGATG 1 cut(s) 584
BtsCI GGATG 4 cut(s) 659, 667, 1108, 1245
BtsIMutI CAGTG 3 cut(s) 383, 405, 1073
BveI ACCTGC 1 cut(s) 814
Cac8I GCNNGC 4 cut(s) 209, 623, 723, 821
CaiI CAGNNNCTG 1 cut(s) 1284
Cfr10I RCCGGY 1 cut(s) 17
Cfr13I GGNCC 3 cut(s) 257, 487, 728
CseI GACGC 4 cut(s) 293, 419, 1043, 1084
Csp6I GTAC 2 cut(s) 1203, 1273
CviAII CATG 6 cut(s) 591, 622, 685, 722, 738, 1003
CviQI GTAC 2 cut(s) 1203, 1273
DdeI CTNAG 7 cut(s) 183, 261, 639, 770, 853, 892, 1199
DpnI GATC 6 cut(s) 58, 173, 345, 636, 759, 907
DpnII GATC 6 cut(s) 56, 171, 343, 634, 757, 905
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
EciI GGCGGA 2 cut(s) 49, 133
Eco130I CCWWGG 1 cut(s) 269
Eco32I GATATC 1 cut(s) 7
Eco47I GGWCC 2 cut(s) 257, 487
Eco81I CCTNAGG 2 cut(s) 639, 853
EcoRI GAATTC 1 cut(s) 333
EcoRII CCWGG 2 cut(s) 158, 880
EcoRV GATATC 1 cut(s) 7
EcoT14I CCWWGG 1 cut(s) 269
EcoT22I ATGCAT 4 cut(s) 554, 623, 925, 1018
ErhI CCWWGG 1 cut(s) 269
FaeI CATG 6 cut(s) 594, 625, 688, 725, 741, 1006
FaqI GGGAC 2 cut(s) 679, 947
FatI CATG 6 cut(s) 590, 621, 684, 721, 737, 1002
FauI CCCGC 2 cut(s) 223, 718
FauNDI CATATG 1 cut(s) 554
FbaI TGATCA 2 cut(s) 171, 757
FblI GTMKAC 1 cut(s) 987
Fnu4HI GCNGC 7 cut(s) 40, 43, 205, 412, 912, 998, 1052
FokI GGATG 4 cut(s) 666, 674, 1115, 1252
Fsp4HI GCNGC 7 cut(s) 40, 43, 205, 412, 912, 998, 1052
FspBI CTAG 2 cut(s) 237, 249
GluI GCNGC 7 cut(s) 40, 43, 205, 412, 912, 998, 1052
GsuI CTGGAG 2 cut(s) 181, 462
HaeIII GGCC 2 cut(s) 730, 1213
HapII CCGG 1 cut(s) 18
HgaI GACGC 4 cut(s) 293, 419, 1043, 1084
Hin1II CATG 6 cut(s) 594, 625, 688, 725, 741, 1006
HincII GTYRAC 1 cut(s) 499
HindII GTYRAC 1 cut(s) 499
HinfI GANTC 4 cut(s) 339, 848, 973, 1281
HpaII CCGG 1 cut(s) 18
HphI GGTGA 5 cut(s) 7, 181, 428, 1088, 1246
Hpy166II GTNNAC 4 cut(s) 499, 695, 988, 1071
Hpy188I TCNGA 6 cut(s) 63, 194, 609, 1062, 1174, 1280
Hpy188III TCNNGA 8 cut(s) 48, 260, 479, 638, 804, 1089, 1237, 1285
Hpy8I GTNNAC 4 cut(s) 499, 695, 988, 1071
Hpy99I CGWCG 1 cut(s) 1024
HpyAV CCTTC 2 cut(s) 604, 1240
HpyCH4III ACNGT 3 cut(s) 169, 378, 1046
HpyCH4IV ACGT 4 cut(s) 936, 992, 1022, 1223
HpyCH4V TGCA 8 cut(s) 99, 153, 552, 621, 923, 997, 1016, 1071
HpyF10VI GCNNNNNNNGC 3 cut(s) 727, 908, 920
HpyF3I CTNAG 7 cut(s) 183, 261, 639, 770, 853, 892, 1199
HpySE526I ACGT 4 cut(s) 936, 992, 1022, 1223
Hsp92II CATG 6 cut(s) 594, 625, 688, 725, 741, 1006
Ksp22I TGATCA 2 cut(s) 171, 757
Kzo9I GATC 6 cut(s) 56, 171, 343, 634, 757, 905
LmnI GCTCC 4 cut(s) 36, 162, 195, 301
Lsp1109I GCAGC 5 cut(s) 51, 54, 898, 1009, 1038
LweI GCATC 4 cut(s) 644, 1003, 1025, 1082
MaeI CTAG 2 cut(s) 237, 249
MaeII ACGT 4 cut(s) 936, 992, 1022, 1223
MaeIII GTNAC 4 cut(s) 318, 356, 626, 733
MalI GATC 6 cut(s) 58, 173, 345, 636, 759, 907
MboI GATC 6 cut(s) 56, 171, 343, 634, 757, 905
MboII GAAGA 6 cut(s) 40, 97, 115, 400, 1049, 1187
MflI RGATCY 1 cut(s) 56
MhlI GDGCHC 3 cut(s) 167, 602, 1073
MluCI AATT 4 cut(s) 333, 833, 1083, 1289
MlyI GAGTC 2 cut(s) 348, 982
MmeI TCCRAC 4 cut(s) 86, 106, 352, 631
Mph1103I ATGCAT 4 cut(s) 554, 623, 925, 1018
MseI TTAA 1 cut(s) 836
MslI CAYNNNNRTG 1 cut(s) 742
MspI CCGG 1 cut(s) 18
MspR9I CCNGG 2 cut(s) 160, 882
Mva1269I GAATGC 2 cut(s) 571, 1194
MvaI CCWGG 2 cut(s) 160, 882
MwoI GCNNNNNNNGC 3 cut(s) 727, 908, 920
NdeI CATATG 1 cut(s) 554
NdeII GATC 6 cut(s) 56, 171, 343, 634, 757, 905
NlaIII CATG 6 cut(s) 594, 625, 688, 725, 741, 1006
NlaIV GGNNCC 2 cut(s) 601, 729
NmuCI GTSAC 1 cut(s) 356
NsiI ATGCAT 4 cut(s) 554, 623, 925, 1018
NspI RCATGY 4 cut(s) 625, 688, 725, 741
PaeI GCATGC 2 cut(s) 625, 725
PciI ACATGT 1 cut(s) 684
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PctI GAATGC 2 cut(s) 571, 1194
PfeI GAWTC 2 cut(s) 848, 1281
PflFI GACNNNGTC 2 cut(s) 378, 485
PkrI GCNGC 7 cut(s) 41, 44, 206, 413, 913, 999, 1053
PleI GAGTC 2 cut(s) 347, 981
PpsI GAGTC 2 cut(s) 347, 981
Ppu21I YACGTR 1 cut(s) 1224
PscI ACATGT 1 cut(s) 684
Psp6I CCWGG 2 cut(s) 158, 880
PspGI CCWGG 2 cut(s) 158, 880
PspN4I GGNNCC 2 cut(s) 601, 729
PspPI GGNCC 3 cut(s) 257, 487, 728
PsrI GAACNNNNNNTAC 2 cut(s) 685, 717
PstNI CAGNNNCTG 1 cut(s) 1284
PsuI RGATCY 1 cut(s) 56
PsyI GACNNNGTC 2 cut(s) 378, 485
RsaI GTAC 2 cut(s) 1204, 1274
RsaNI GTAC 2 cut(s) 1203, 1273
RseI CAYNNNNRTG 1 cut(s) 742
SaqAI TTAA 1 cut(s) 836
SatI GCNGC 7 cut(s) 40, 43, 205, 412, 912, 998, 1052
Sau3AI GATC 6 cut(s) 56, 171, 343, 634, 757, 905
Sau96I GGNCC 3 cut(s) 257, 487, 728
SchI GAGTC 2 cut(s) 348, 982
ScrFI CCNGG 2 cut(s) 160, 882
SduI GDGCHC 3 cut(s) 167, 602, 1073
SfaNI GCATC 4 cut(s) 644, 1003, 1025, 1082
SgrAI CRCCGGYG 1 cut(s) 17
SinI GGWCC 2 cut(s) 257, 487
SmiMI CAYNNNNRTG 1 cut(s) 742
SmlI CTYRAG 2 cut(s) 46, 349
SmoI CTYRAG 2 cut(s) 46, 349
SphI GCATGC 2 cut(s) 625, 725
Sse9I AATT 4 cut(s) 333, 833, 1083, 1289
SsiI CCGC 8 cut(s) 34, 144, 205, 230, 411, 414, 725, 1028
SspMI CTAG 2 cut(s) 237, 249
StyD4I CCNGG 2 cut(s) 158, 880
StyI CCWWGG 1 cut(s) 269
TaaI ACNGT 3 cut(s) 169, 378, 1046
TaiI ACGT 4 cut(s) 939, 995, 1025, 1226
TaqI TCGA 8 cut(s) 117, 126, 331, 337, 342, 382, 1019, 1293
TasI AATT 4 cut(s) 333, 833, 1083, 1289
TatI WGTACW 1 cut(s) 1272
TauI GCSGC 2 cut(s) 207, 414
TfiI GAWTC 2 cut(s) 848, 1281
Tru1I TTAA 1 cut(s) 836
Tru9I TTAA 1 cut(s) 836
TscAI CASTG 3 cut(s) 383, 412, 1073
TseFI GTSAC 1 cut(s) 356
TseI GCWGC 5 cut(s) 39, 42, 911, 997, 1051
Tsp45I GTSAC 1 cut(s) 356
TspDTI ATGAA 4 cut(s) 528, 768, 801, 962
TspRI CASTG 3 cut(s) 383, 412, 1073
Tth111I GACNNNGTC 2 cut(s) 378, 485
VneI GTGCAC 1 cut(s) 1069
VpaK11BI GGWCC 2 cut(s) 257, 487
XapI RAATTY 2 cut(s) 333, 1289
XceI RCATGY 4 cut(s) 625, 688, 725, 741
XmiI GTMKAC 1 cut(s) 987
XspI CTAG 2 cut(s) 237, 249
Zsp2I ATGCAT 4 cut(s) 554, 623, 925, 1018
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.