Rh1BG028800

F-box WD-40 repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0004801
Physical Location & Seq
Forward (+)
3961785 .. 3967120
5336 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG028800.1

Sequence Viewer

Length: 1230 bp
ATGGATCCCAGTCGCGTCGGCAGCAAGAAGAGGCCAACCAGAAGCTCAAGAGCTCCGGCCAGAATCCAATCTCTCGACCACGACATCCTCTGTATCATCTTCTCTTTCCTCGACTTCTTCGACCTCGCACGTTGTTCCGTCGTCTGCAAAAGCTGGAGTGGTATTATCAACAAGTCAAAGTTGCTAGAAGCGCTTTACTACAAGAAGTGGCAGATGGGTTCTGAGAACAGGTTGGGTTCATGGGTTCATGTTGATCACTGGAGAGCTCACTCTGTTGGGGTTGATCAGTGCCGAAAGAAGATGGGTTTGCTTCTTACTGGCATTGGTGATAAGGTTATGCGCCTTTGGTCATTGGAAAGCTATAAATGTGTGGGAGAATATCCCATTCCAGACAAAGTCCCTTTAGTTGACTTTGATTTTGATGAGAGCAAGATTGTTGGTTTGGTCGCCAGTCGTATATGCATATGGAGGCGGAATGGGATAAGAAGTATATTTCCCTCGCGTGAGGGCACCTTTCCAAAGGGCTTATGCATGCGTTACTGCGATCCTGATGCTGTGGTTGGCTGTGTGGATGGGACAGCTCGTGTATTTGACATGTACAGTCAACAATGTTCTCAAGTCGTAAAAATGCATGCTGGTCCAGTGACATGCTTGTGTTTGAGTGATGATCAGTTGATACTTAGCGGTTCTTCTCTTGGCAGTATCACGGTGTCTAATCTTTTATCTGATCAGCGGGTTGCAGAATTAAGATCGACACATTCCACAGGCATAAGGACTTTGTGTTTCAACCCCTCATCTCACCAGCTGTTCGCTGGATCAACTGCTGGATATGTATTTTGTTGGGACCTCAGGACAAGGAGATGTTTATGGGAGACACGAGTGAGTCCAAATGAGGTATATTCAATGCAACACCTGAGAAATGATGCATCAAGGTTGGTGGTTGGTGGAATAGATGGTGTGCTACGTATACTGATTCAGAACACAGGTGAAGTTGTTTCAAGATTTGTTTTGGAAGGCAACTTATTGTCAAGTTCAAGTTCAAGTTCTCATCAAAACGTAGTTGGAAGTGGCACTGTCCAAAGAATGACAGGACGAAGGCTTTCACAAAATGCCTCAATTGATAGCATTCCTAGAAGCAGTCGACCTCCCATCACTTGCTTAGCGGTCGGGATGAAGAGGGTTATCACCACACACAATAATAAGTACATCAGAATGTGGAAGTTCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
F-box F-box-like WD40

Protein Analysis

409

Amino Acids

45.8

Weight (kDa)

9.46

Isoelectric Point (pI)

43.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 22 - 61 1.2e-09 F-box domain
F-box-like PF12937 27 - 61 6.1e-09 F-box-like
Beta-prop_EML PF23409 110 - 276 1.1e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_HPS5 PF23756 174 - 288 4.1e-06 HPS5 beta-propeller
Beta-prop_EML_2 PF23414 175 - 274 5.1e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_ACSF4 PF13570 176 - 355 1.1e-12 Beta-alanine-activating enzyme, beta-propeller
WD40_WDHD1_1st PF24817 176 - 301 3.1e-10 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 185 - 323 2.5e-18 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 186 - 359 3.5e-13 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 186 - 267 5.1e-06 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 186 - 320 6.1e-09 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 187 - 334 9.3e-10 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 189 - 341 9.4e-15 WDR5 beta-propeller domain
WDR55 PF24796 190 - 335 7.8e-11 WDR55
Beta-prop_THOC3 PF25174 226 - 340 2.1e-07 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 259 - 342 5.2e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 509
AccI GTMKAC 2 cut(s) 967, 1141
AccII CGCG 2 cut(s) 15, 502
AciI CCGC 4 cut(s) 472, 684, 733, 1163
AclWI GGATC 3 cut(s) 12, 539, 823
AcoI YGGCCR 1 cut(s) 57
AfaI GTAC 2 cut(s) 599, 1205
AfeI AGCGCT 1 cut(s) 192
AflIII ACRYGT 1 cut(s) 594
AgsI TTSAA 6 cut(s) 787, 903, 999, 1035, 1041, 1225
AluBI AGCT 7 cut(s) 45, 53, 153, 266, 360, 581, 805
AluI AGCT 7 cut(s) 45, 53, 153, 266, 360, 581, 805
Alw21I GWGCWC 2 cut(s) 55, 268
Alw26I GTCTC 1 cut(s) 866
AlwI GGATC 3 cut(s) 12, 539, 823
Aor51HI AGCGCT 1 cut(s) 192
AoxI GGCC 2 cut(s) 32, 57
ApeKI GCWGC 1 cut(s) 21
ArsI GACNNNNNNTTYG 2 cut(s) 401, 433
Asp700I GAANNNNTTC 1 cut(s) 1099
AspLEI GCGC 2 cut(s) 193, 342
AspS9I GGNCC 2 cut(s) 638, 844
AsuHPI GGTGA 4 cut(s) 338, 791, 998, 1177
AvaII GGWCC 2 cut(s) 638, 844
AxyI CCTNAGG 1 cut(s) 848
BaeGI GKGCMC 1 cut(s) 512
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 509
BanII GRGCYC 2 cut(s) 55, 268
BauI CACGAG 2 cut(s) 582, 876
Bbv12I GWGCWC 2 cut(s) 55, 268
BbvI GCAGC 1 cut(s) 33
BccI CCATC 5 cut(s) 208, 295, 566, 947, 1157
BcgI CGANNNNNNTGC 2 cut(s) 533, 567
BclI TGATCA 4 cut(s) 253, 283, 667, 727
BcoDI GTCTC 1 cut(s) 866
BfaI CTAG 2 cut(s) 185, 1131
BfoI RGCGCY 1 cut(s) 194
BisI GCNGC 1 cut(s) 22
BlpI GCTNAGC 1 cut(s) 1159
BlsI GCNGC 1 cut(s) 23
Bme18I GGWCC 2 cut(s) 638, 844
BmgT120I GGNCC 2 cut(s) 638, 844
BmiI GGNNCC 3 cut(s) 6, 511, 845
BmrI ACTGGG 1 cut(s) 3
BmsI GCATC 3 cut(s) 541, 913, 935
BmuI ACTGGG 1 cut(s) 3
BpmI CTGGAG 2 cut(s) 175, 280
Bpu1102I GCTNAGC 1 cut(s) 1159
BpuEI CTTGAG 2 cut(s) 31, 600
BsaAI YACGTR 1 cut(s) 965
Bse1I ACTGG 5 cut(s) 9, 263, 322, 450, 641
Bse21I CCTNAGG 1 cut(s) 848
BseGI GGATG 3 cut(s) 84, 577, 1176
BseMII CTCAG 3 cut(s) 213, 862, 905
BseNI ACTGG 5 cut(s) 9, 263, 322, 450, 641
BseSI GKGCMC 1 cut(s) 512
BseXI GCAGC 1 cut(s) 33
Bsh1236I CGCG 2 cut(s) 15, 502
Bsh1285I CGRYCG 1 cut(s) 1167
BshFI GGCC 2 cut(s) 34, 59
BshNI GGYRCC 1 cut(s) 509
BsiEI CGRYCG 1 cut(s) 1167
BsiHKAI GWGCWC 2 cut(s) 55, 268
BsiSI CCGG 1 cut(s) 56
BslFI GGGAC 3 cut(s) 383, 589, 857
BsmAI GTCTC 1 cut(s) 866
BsmFI GGGAC 3 cut(s) 383, 589, 857
BsmI GAATGC 1 cut(s) 1125
BsnI GGCC 2 cut(s) 34, 59
Bsp1286I GDGCHC 3 cut(s) 55, 268, 512
Bsp1407I TGTACA 1 cut(s) 597
Bsp143I GATC 8 cut(s) 4, 253, 283, 544, 667, 727, 749, 815
Bsp1720I GCTNAGC 1 cut(s) 1159
BspACI CCGC 4 cut(s) 472, 684, 733, 1163
BspANI GGCC 2 cut(s) 34, 59
BspCNI CTCAG 3 cut(s) 214, 861, 906
BspFNI CGCG 2 cut(s) 15, 502
BspLI GGNNCC 3 cut(s) 6, 511, 845
BspPI GGATC 3 cut(s) 12, 539, 823
BspT107I GGYRCC 1 cut(s) 509
BsrGI TGTACA 1 cut(s) 597
BsrI ACTGG 5 cut(s) 9, 263, 322, 450, 641
BssMI GATC 8 cut(s) 4, 253, 283, 544, 667, 727, 749, 815
BssNAI GTATAC 1 cut(s) 968
BssSI CACGAG 2 cut(s) 582, 876
Bst1107I GTATAC 1 cut(s) 968
Bst2BI CACGAG 2 cut(s) 582, 876
Bst4CI ACNGT 3 cut(s) 602, 709, 1075
Bst6I CTCTTC 2 cut(s) 23, 1169
BstAUI TGTACA 1 cut(s) 597
BstBAI YACGTR 1 cut(s) 965
BstC8I GCNNGC 2 cut(s) 533, 633
BstDEI CTNAG 5 cut(s) 222, 680, 848, 914, 1159
BstF5I GGATG 3 cut(s) 84, 577, 1176
BstFNI CGCG 2 cut(s) 15, 502
BstH2I RGCGCY 1 cut(s) 194
BstHHI GCGC 2 cut(s) 193, 342
BstKTI GATC 8 cut(s) 7, 256, 286, 547, 670, 730, 752, 818
BstMAI GTCTC 1 cut(s) 866
BstMBI GATC 8 cut(s) 4, 253, 283, 544, 667, 727, 749, 815
BstMCI CGRYCG 1 cut(s) 1167
BstMWI GCNNNNNNNGC 2 cut(s) 21, 190
BstNSI RCATGY 4 cut(s) 535, 598, 635, 651
BstSLI GKGCMC 1 cut(s) 512
BstSNI TACGTA 1 cut(s) 965
BstUI CGCG 2 cut(s) 15, 502
BstV1I GCAGC 1 cut(s) 33
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BstZ17I GTATAC 1 cut(s) 968
Bsu36I CCTNAGG 1 cut(s) 848
BsuRI GGCC 2 cut(s) 34, 59
BtsCI GGATG 3 cut(s) 84, 577, 1176
BtsIMutI CAGTG 4 cut(s) 256, 293, 648, 1071
Cac8I GCNNGC 2 cut(s) 533, 633
CfoI GCGC 2 cut(s) 193, 342
Cfr13I GGNCC 2 cut(s) 638, 844
CseI GACGC 1 cut(s) 4
Csp6I GTAC 2 cut(s) 598, 1204
CspCI CAANNNNNGTGG 2 cut(s) 918, 953
CviAII CATG 6 cut(s) 240, 248, 532, 595, 632, 648
CviQI GTAC 2 cut(s) 598, 1204
DdeI CTNAG 5 cut(s) 222, 680, 848, 914, 1159
DpnI GATC 8 cut(s) 6, 255, 285, 546, 669, 729, 751, 817
DpnII GATC 8 cut(s) 4, 253, 283, 544, 667, 727, 749, 815
EaeI YGGCCR 1 cut(s) 57
Eam1104I CTCTTC 2 cut(s) 23, 1169
EarI CTCTTC 2 cut(s) 23, 1169
EciI GGCGGA 1 cut(s) 487
Ecl136II GAGCTC 2 cut(s) 53, 266
Eco105I TACGTA 1 cut(s) 965
Eco24I GRGCYC 2 cut(s) 55, 268
Eco47I GGWCC 2 cut(s) 638, 844
Eco47III AGCGCT 1 cut(s) 192
Eco53kI GAGCTC 2 cut(s) 53, 266
Eco81I CCTNAGG 1 cut(s) 848
EcoICRI GAGCTC 2 cut(s) 53, 266
EcoO109I RGGNCCY 1 cut(s) 844
EcoT22I ATGCAT 4 cut(s) 464, 533, 633, 928
EcoT38I GRGCYC 2 cut(s) 55, 268
FaeI CATG 6 cut(s) 243, 251, 535, 598, 635, 651
FaqI GGGAC 3 cut(s) 383, 589, 857
FatI CATG 6 cut(s) 239, 247, 531, 594, 631, 647
FauI CCCGC 1 cut(s) 726
FauNDI CATATG 1 cut(s) 464
FbaI TGATCA 4 cut(s) 253, 283, 667, 727
FblI GTMKAC 2 cut(s) 967, 1141
Fnu4HI GCNGC 1 cut(s) 22
FokI GGATG 3 cut(s) 71, 584, 1183
FriOI GRGCYC 2 cut(s) 55, 268
Fsp4HI GCNGC 1 cut(s) 22
FspBI CTAG 2 cut(s) 185, 1131
GlaI GCGC 2 cut(s) 192, 341
GluI GCNGC 1 cut(s) 22
GsuI CTGGAG 2 cut(s) 175, 280
HaeII RGCGCY 1 cut(s) 194
HaeIII GGCC 2 cut(s) 34, 59
HapII CCGG 1 cut(s) 56
HgaI GACGC 1 cut(s) 4
HhaI GCGC 2 cut(s) 193, 342
Hin1II CATG 6 cut(s) 243, 251, 535, 598, 635, 651
Hin6I GCGC 2 cut(s) 191, 340
HinP1I GCGC 2 cut(s) 191, 340
HincII GTYRAC 3 cut(s) 409, 605, 1142
HindII GTYRAC 3 cut(s) 409, 605, 1142
HinfI GANTC 3 cut(s) 63, 883, 973
HpaII CCGG 1 cut(s) 56
HphI GGTGA 4 cut(s) 338, 791, 998, 1177
Hpy166II GTNNAC 4 cut(s) 409, 605, 968, 1142
Hpy188I TCNGA 4 cut(s) 223, 727, 978, 1211
Hpy188III TCNNGA 7 cut(s) 48, 74, 389, 548, 850, 999, 1168
Hpy8I GTNNAC 4 cut(s) 409, 605, 968, 1142
Hpy99I CGWCG 2 cut(s) 20, 143
HpyAV CCTTC 2 cut(s) 1007, 1089
HpyCH4III ACNGT 3 cut(s) 602, 709, 1075
HpyCH4IV ACGT 3 cut(s) 130, 964, 1056
HpyCH4V TGCA 7 cut(s) 147, 462, 531, 631, 740, 907, 926
HpyF10VI GCNNNNNNNGC 2 cut(s) 21, 190
HpyF3I CTNAG 5 cut(s) 222, 680, 848, 914, 1159
HpySE526I ACGT 3 cut(s) 130, 964, 1056
Hsp92II CATG 6 cut(s) 243, 251, 535, 598, 635, 651
HspAI GCGC 2 cut(s) 191, 340
Ksp22I TGATCA 4 cut(s) 253, 283, 667, 727
Kzo9I GATC 8 cut(s) 4, 253, 283, 544, 667, 727, 749, 815
LmnI GCTCC 1 cut(s) 58
Lsp1109I GCAGC 1 cut(s) 33
LweI GCATC 3 cut(s) 541, 913, 935
MaeI CTAG 2 cut(s) 185, 1131
MaeII ACGT 3 cut(s) 130, 964, 1056
MaeIII GTNAC 2 cut(s) 536, 643
MalI GATC 8 cut(s) 6, 255, 285, 546, 669, 729, 751, 817
MboI GATC 8 cut(s) 4, 253, 283, 544, 667, 727, 749, 815
MboII GAAGA 6 cut(s) 40, 91, 109, 310, 681, 1186
MfeI CAATTG 1 cut(s) 1116
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 3 cut(s) 55, 268, 512
MluCI AATT 2 cut(s) 743, 1116
MlyI GAGTC 1 cut(s) 892
MmeI TCCRAC 1 cut(s) 1042
Mph1103I ATGCAT 4 cut(s) 464, 533, 633, 928
MroXI GAANNNNTTC 1 cut(s) 1099
MseI TTAA 1 cut(s) 746
MslI CAYNNNNRTG 2 cut(s) 652, 1211
MspA1I CMGCKG 2 cut(s) 733, 805
MspI CCGG 1 cut(s) 56
MunI CAATTG 1 cut(s) 1116
Mva1269I GAATGC 1 cut(s) 1125
MvnI CGCG 2 cut(s) 15, 502
MwoI GCNNNNNNNGC 2 cut(s) 21, 190
NdeI CATATG 1 cut(s) 464
NdeII GATC 8 cut(s) 4, 253, 283, 544, 667, 727, 749, 815
NlaIII CATG 6 cut(s) 243, 251, 535, 598, 635, 651
NlaIV GGNNCC 3 cut(s) 6, 511, 845
NmuCI GTSAC 1 cut(s) 643
NsiI ATGCAT 4 cut(s) 464, 533, 633, 928
NspI RCATGY 4 cut(s) 535, 598, 635, 651
PaeI GCATGC 2 cut(s) 535, 635
PciI ACATGT 1 cut(s) 594
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PctI GAATGC 1 cut(s) 1125
PdmI GAANNNNTTC 1 cut(s) 1099
PfeI GAWTC 2 cut(s) 63, 973
PflFI GACNNNGTC 1 cut(s) 395
PkrI GCNGC 1 cut(s) 23
PleI GAGTC 1 cut(s) 891
PpsI GAGTC 1 cut(s) 891
Ppu21I YACGTR 1 cut(s) 965
PpuMI RGGWCCY 1 cut(s) 844
PscI ACATGT 1 cut(s) 594
Psp124BI GAGCTC 2 cut(s) 55, 268
Psp5II RGGWCCY 1 cut(s) 844
PspN4I GGNNCC 3 cut(s) 6, 511, 845
PspPI GGNCC 2 cut(s) 638, 844
PspPPI RGGWCCY 1 cut(s) 844
PsuI RGATCY 1 cut(s) 4
PsyI GACNNNGTC 1 cut(s) 395
PvuII CAGCTG 1 cut(s) 805
RsaI GTAC 2 cut(s) 599, 1205
RsaNI GTAC 2 cut(s) 598, 1204
RseI CAYNNNNRTG 2 cut(s) 652, 1211
SacI GAGCTC 2 cut(s) 55, 268
SalI GTCGAC 1 cut(s) 1140
SaqAI TTAA 1 cut(s) 746
SatI GCNGC 1 cut(s) 22
Sau3AI GATC 8 cut(s) 4, 253, 283, 544, 667, 727, 749, 815
Sau96I GGNCC 2 cut(s) 638, 844
SchI GAGTC 1 cut(s) 892
SduI GDGCHC 3 cut(s) 55, 268, 512
SfaNI GCATC 3 cut(s) 541, 913, 935
SinI GGWCC 2 cut(s) 638, 844
SmiMI CAYNNNNRTG 2 cut(s) 652, 1211
SmlI CTYRAG 2 cut(s) 46, 615
SmoI CTYRAG 2 cut(s) 46, 615
SnaBI TACGTA 1 cut(s) 965
SphI GCATGC 2 cut(s) 535, 635
Sse9I AATT 2 cut(s) 743, 1116
SsiI CCGC 4 cut(s) 472, 684, 733, 1163
SspMI CTAG 2 cut(s) 185, 1131
SstI GAGCTC 2 cut(s) 55, 268
TaaI ACNGT 3 cut(s) 602, 709, 1075
TaiI ACGT 3 cut(s) 133, 967, 1059
TaqI TCGA 5 cut(s) 75, 111, 120, 752, 1141
TasI AATT 2 cut(s) 743, 1116
TatI WGTACW 2 cut(s) 597, 1203
TfiI GAWTC 2 cut(s) 63, 973
Tru1I TTAA 1 cut(s) 746
Tru9I TTAA 1 cut(s) 746
TscAI CASTG 4 cut(s) 263, 293, 648, 1078
TseFI GTSAC 1 cut(s) 643
TseI GCWGC 1 cut(s) 21
Tsp45I GTSAC 1 cut(s) 643
TspDTI ATGAA 3 cut(s) 228, 236, 1187
TspGWI ACGGA 1 cut(s) 127
TspRI CASTG 4 cut(s) 263, 293, 648, 1078
Tth111I GACNNNGTC 1 cut(s) 395
VpaK11BI GGWCC 2 cut(s) 638, 844
XceI RCATGY 4 cut(s) 535, 598, 635, 651
XcmI CCANNNNNNNNNTGG 1 cut(s) 809
XmiI GTMKAC 2 cut(s) 967, 1141
XmnI GAANNNNTTC 1 cut(s) 1099
XspI CTAG 2 cut(s) 185, 1131
Zsp2I ATGCAT 4 cut(s) 464, 533, 633, 928
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.