Rroxscaffold_4G00329280

F-box WD-40 repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0004801
Physical Location & Seq
Reverse (-)
62518635 .. 62524858
6224 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00329280.1

Sequence Viewer

Length: 1365 bp
ATGGATCCTAAGAAGAGGCCAACCAGAAGCTCAAGAGCTCCGGCCAGAATCCAATCTCTCGACCACGACATCCTTTGTATCATCTTCTCTTTTCTCGACTTCTTCGACCTGGCACGTTGTTCCGTCGTCTGCAAAAGCTGGAGTGGTATTATCAACAAGTCAAAGCTGCTAGAAGCGCTTTACTACAAGAAGTGGCAGATGGGTCTTGTGGGTTCTGAGAATAGGTCTAGTGGGTGCCCAGAGAAATCTTTGAATGTTTATTTGGAGGAGATGGCTCTGCGGCATCATAGACTGGCTTTACAGCAAGGTCGGGTTCATGTTGATCACTGGAGAGCTCACTCTGTTGGGGTTGATCAGTGCCGAAAGAAGATGGGTTTGCTTCTTACTGGCATTGGTGATAAGGTTATGCGCCTTTGGTCATTGGAAAGCTATAAATGTGTTGGAGAATATCCCATTCCAGACAAAGTCCCTTTAGTTGACTTTGATTTTGATGAGAGCAAGATCCATGTGAGCCATACCCTGAATAGATTGACTTTGAGTGTCCAGATTGTGAAGCAAGACTGTCAGATTGTTGGTTTGGTCGGCAGTCGTATATGCATATGGAGGCGGAATGGGATAAGAAGTATATTTCCCTTGCGTGAGGGCACCTTTCCAAAGGGCTTATGCATGCGTTACTGCGATCCTGATGCTGTGGTTGGCTGTGTGGATGGGACAGCTCGTGTGTTTGACATGTACAGTCAACAATGTTCTCAAATCGTTAAAATGCATGCTGGTCCAGTGACATGCTTATGTTTGAGTGATGATCAGTTGATACTTAGCGGTTCTTCTCTTGGCAGTATCACTGTGTCCGGACTTTTATCTGATCAGCTGGTTGCAGAATTAAGATCGACACGTTCCACAGACATAAGGACTTTGTGTTACAACCCCTCATCTCGCCGGCTGTTCGCTGGATCAACTGATGGATATGTATTTTGTTGGGACCTTAGGACAAGGAGATGTTTATGGGAGACAAGAGTGAGTCCAAATGTGGTATATTCAATGCAACACCTGAGAAATGATGCATCAAGCTTGGTGGTTGGTGGAATAGATGGTGTGCTACGTATACTGAATCAGAATACAGGTGAAGTAGTTTCAAGGTTTGTTTTGGAAGGCAACTTATTGTCAAGTTCAAGTTCAAGTTCTCATCAAAACGTAGTTGGAAGTGGCACCGTCCAAAGAATGACAGGACGAAGGCTTTCACAAAATGCCTCAATTGATAGCATTCCTAGAAGCAGTCGACCTCCCATCACTTGCTTAGCGGTCGGGATGAAGAAGGTTATCACCACACACAATAATAAGTACATCAGAACGTGGAAGTTCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
F-box F-box-like WD40

Protein Analysis

454

Amino Acids

50.91

Weight (kDa)

9.38

Isoelectric Point (pI)

46.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 17 - 56 1.4e-09 F-box domain
F-box-like PF12937 22 - 56 7e-09 F-box-like
Beta-prop_HPS5 PF23756 211 - 341 3.5e-06 HPS5 beta-propeller
Beta-prop_EML_2 PF23414 220 - 323 1.4e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_ACSF4 PF13570 221 - 399 6.6e-13 Beta-alanine-activating enzyme, beta-propeller
WD40_WDHD1_1st PF24817 222 - 346 6e-09 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 230 - 368 1.1e-16 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 230 - 402 1.5e-13 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 230 - 380 1.9e-10 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 234 - 386 8.3e-15 WDR5 beta-propeller domain
WDR55 PF24796 269 - 450 2.2e-07 WDR55
Beta-prop_THOC3 PF25174 271 - 386 1.6e-07 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 304 - 388 6e-08 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 234, 644, 1205
AccI GTMKAC 2 cut(s) 1102, 1276
AccIII TCCGGA 1 cut(s) 848
AciI CCGC 4 cut(s) 280, 607, 819, 1298
AclWI GGATC 4 cut(s) 12, 496, 674, 958
AcoI YGGCCR 1 cut(s) 42
AfaI GTAC 2 cut(s) 734, 1340
AfeI AGCGCT 1 cut(s) 177
AflIII ACRYGT 2 cut(s) 729, 890
AgsI TTSAA 6 cut(s) 253, 1038, 1134, 1170, 1176, 1360
AjnI CCWGG 1 cut(s) 108
AjuI GAANNNNNNNTTGG 2 cut(s) 245, 277
AluBI AGCT 9 cut(s) 30, 38, 138, 166, 335, 429, 716, 868, 1068
AluI AGCT 9 cut(s) 30, 38, 138, 166, 335, 429, 716, 868, 1068
Alw21I GWGCWC 2 cut(s) 40, 337
Alw26I GTCTC 1 cut(s) 1001
AlwI GGATC 4 cut(s) 12, 496, 674, 958
Aor13HI TCCGGA 1 cut(s) 848
Aor51HI AGCGCT 1 cut(s) 177
AoxI GGCC 2 cut(s) 17, 42
ApeKI GCWGC 1 cut(s) 166
ArsI GACNNNNNNTTYG 2 cut(s) 470, 502
Asp700I GAANNNNTTC 1 cut(s) 1234
AspLEI GCGC 2 cut(s) 178, 411
AspS9I GGNCC 2 cut(s) 773, 979
AsuHPI GGTGA 3 cut(s) 407, 1133, 1312
AvaII GGWCC 2 cut(s) 773, 979
AxyI CCTNAGG 1 cut(s) 983
BaeGI GKGCMC 2 cut(s) 239, 647
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 3 cut(s) 234, 644, 1205
BanII GRGCYC 2 cut(s) 40, 337
BauI CACGAG 1 cut(s) 717
Bbv12I GWGCWC 2 cut(s) 40, 337
BbvI GCAGC 1 cut(s) 153
BccI CCATC 7 cut(s) 193, 265, 364, 701, 953, 1082, 1292
BcgI CGANNNNNNTGC 2 cut(s) 668, 702
BciT130I CCWGG 1 cut(s) 110
BclI TGATCA 4 cut(s) 322, 352, 802, 862
BcoDI GTCTC 1 cut(s) 1001
BfaI CTAG 3 cut(s) 170, 228, 1266
BfoI RGCGCY 1 cut(s) 179
BisI GCNGC 2 cut(s) 167, 281
BlpI GCTNAGC 1 cut(s) 1294
BlsI GCNGC 2 cut(s) 168, 282
Bme1390I CCNGG 1 cut(s) 110
Bme18I GGWCC 2 cut(s) 773, 979
BmgT120I GGNCC 2 cut(s) 773, 979
BmiI GGNNCC 5 cut(s) 6, 236, 646, 980, 1207
BmrFI CCNGG 1 cut(s) 110
BmsI GCATC 4 cut(s) 292, 676, 1048, 1070
BpmI CTGGAG 2 cut(s) 160, 349
Bpu1102I GCTNAGC 1 cut(s) 1294
BpuEI CTTGAG 1 cut(s) 16
BsaAI YACGTR 1 cut(s) 1100
BsaWI WCCGGW 1 cut(s) 848
Bse118I RCCGGY 1 cut(s) 936
Bse1I ACTGG 4 cut(s) 297, 332, 391, 776
Bse21I CCTNAGG 1 cut(s) 983
BseAI TCCGGA 1 cut(s) 848
BseBI CCWGG 1 cut(s) 110
BseGI GGATG 3 cut(s) 69, 712, 1311
BseMII CTCAG 2 cut(s) 207, 1040
BseNI ACTGG 4 cut(s) 297, 332, 391, 776
BseRI GAGGAG 1 cut(s) 281
BseSI GKGCMC 2 cut(s) 239, 647
BseXI GCAGC 1 cut(s) 153
Bsh1285I CGRYCG 1 cut(s) 1302
BshFI GGCC 2 cut(s) 19, 44
BshNI GGYRCC 3 cut(s) 234, 644, 1205
BsiEI CGRYCG 1 cut(s) 1302
BsiHKAI GWGCWC 2 cut(s) 40, 337
BsiSI CCGG 3 cut(s) 41, 849, 937
BslFI GGGAC 3 cut(s) 452, 724, 992
BsmAI GTCTC 1 cut(s) 1001
BsmFI GGGAC 3 cut(s) 452, 724, 992
BsmI GAATGC 1 cut(s) 1260
BsnI GGCC 2 cut(s) 19, 44
Bsp1286I GDGCHC 4 cut(s) 40, 239, 337, 647
Bsp13I TCCGGA 1 cut(s) 848
Bsp1407I TGTACA 1 cut(s) 732
Bsp143I GATC 9 cut(s) 4, 322, 352, 501, 679, 802, 862, 884, 950
Bsp1720I GCTNAGC 1 cut(s) 1294
BspACI CCGC 4 cut(s) 280, 607, 819, 1298
BspANI GGCC 2 cut(s) 19, 44
BspCNI CTCAG 2 cut(s) 208, 1041
BspEI TCCGGA 1 cut(s) 848
BspLI GGNNCC 5 cut(s) 6, 236, 646, 980, 1207
BspPI GGATC 4 cut(s) 12, 496, 674, 958
BspT107I GGYRCC 3 cut(s) 234, 644, 1205
BsrFI RCCGGY 1 cut(s) 936
BsrGI TGTACA 1 cut(s) 732
BsrI ACTGG 4 cut(s) 297, 332, 391, 776
BssAI RCCGGY 1 cut(s) 936
BssMI GATC 9 cut(s) 4, 322, 352, 501, 679, 802, 862, 884, 950
BssNAI GTATAC 1 cut(s) 1103
BssSI CACGAG 1 cut(s) 717
Bst1107I GTATAC 1 cut(s) 1103
Bst2BI CACGAG 1 cut(s) 717
Bst2UI CCWGG 1 cut(s) 110
Bst4CI ACNGT 4 cut(s) 563, 737, 844, 1210
Bst6I CTCTTC 1 cut(s) 8
BstAUI TGTACA 1 cut(s) 732
BstBAI YACGTR 1 cut(s) 1100
BstC8I GCNNGC 3 cut(s) 668, 768, 938
BstDEI CTNAG 6 cut(s) 9, 216, 815, 983, 1049, 1294
BstF5I GGATG 3 cut(s) 69, 712, 1311
BstH2I RGCGCY 1 cut(s) 179
BstHHI GCGC 2 cut(s) 178, 411
BstKTI GATC 9 cut(s) 7, 325, 355, 504, 682, 805, 865, 887, 953
BstMAI GTCTC 1 cut(s) 1001
BstMBI GATC 9 cut(s) 4, 322, 352, 501, 679, 802, 862, 884, 950
BstMCI CGRYCG 1 cut(s) 1302
BstMWI GCNNNNNNNGC 1 cut(s) 175
BstNI CCWGG 1 cut(s) 110
BstNSI RCATGY 4 cut(s) 670, 733, 770, 786
BstSCI CCNGG 1 cut(s) 108
BstSLI GKGCMC 2 cut(s) 239, 647
BstSNI TACGTA 1 cut(s) 1100
BstV1I GCAGC 1 cut(s) 153
BstX2I RGATCY 2 cut(s) 4, 501
BstYI RGATCY 2 cut(s) 4, 501
BstZ17I GTATAC 1 cut(s) 1103
Bsu36I CCTNAGG 1 cut(s) 983
BsuRI GGCC 2 cut(s) 19, 44
BtsCI GGATG 3 cut(s) 69, 712, 1311
BtsIMutI CAGTG 4 cut(s) 325, 362, 783, 840
Cac8I GCNNGC 3 cut(s) 668, 768, 938
CfoI GCGC 2 cut(s) 178, 411
Cfr10I RCCGGY 1 cut(s) 936
Cfr13I GGNCC 2 cut(s) 773, 979
Csp6I GTAC 2 cut(s) 733, 1339
CspCI CAANNNNNGTGG 2 cut(s) 1053, 1088
CviAII CATG 6 cut(s) 317, 506, 667, 730, 767, 783
CviQI GTAC 2 cut(s) 733, 1339
DdeI CTNAG 6 cut(s) 9, 216, 815, 983, 1049, 1294
DpnI GATC 9 cut(s) 6, 324, 354, 503, 681, 804, 864, 886, 952
DpnII GATC 9 cut(s) 4, 322, 352, 501, 679, 802, 862, 884, 950
EaeI YGGCCR 1 cut(s) 42
Eam1104I CTCTTC 1 cut(s) 8
EarI CTCTTC 1 cut(s) 8
EciI GGCGGA 1 cut(s) 622
Ecl136II GAGCTC 2 cut(s) 38, 335
Eco105I TACGTA 1 cut(s) 1100
Eco24I GRGCYC 2 cut(s) 40, 337
Eco47I GGWCC 2 cut(s) 773, 979
Eco47III AGCGCT 1 cut(s) 177
Eco53kI GAGCTC 2 cut(s) 38, 335
Eco81I CCTNAGG 1 cut(s) 983
EcoICRI GAGCTC 2 cut(s) 38, 335
EcoO109I RGGNCCY 1 cut(s) 979
EcoRII CCWGG 1 cut(s) 108
EcoT22I ATGCAT 4 cut(s) 599, 668, 768, 1063
EcoT38I GRGCYC 2 cut(s) 40, 337
FaeI CATG 6 cut(s) 320, 509, 670, 733, 770, 786
FaqI GGGAC 3 cut(s) 452, 724, 992
FatI CATG 6 cut(s) 316, 505, 666, 729, 766, 782
FauNDI CATATG 1 cut(s) 599
FbaI TGATCA 4 cut(s) 322, 352, 802, 862
FblI GTMKAC 2 cut(s) 1102, 1276
Fnu4HI GCNGC 2 cut(s) 167, 281
FokI GGATG 3 cut(s) 56, 719, 1318
FriOI GRGCYC 2 cut(s) 40, 337
Fsp4HI GCNGC 2 cut(s) 167, 281
FspBI CTAG 3 cut(s) 170, 228, 1266
GlaI GCGC 2 cut(s) 177, 410
GluI GCNGC 2 cut(s) 167, 281
GsuI CTGGAG 2 cut(s) 160, 349
HaeII RGCGCY 1 cut(s) 179
HaeIII GGCC 2 cut(s) 19, 44
HapII CCGG 3 cut(s) 41, 849, 937
HhaI GCGC 2 cut(s) 178, 411
Hin1II CATG 6 cut(s) 320, 509, 670, 733, 770, 786
Hin6I GCGC 2 cut(s) 176, 409
HinP1I GCGC 2 cut(s) 176, 409
HincII GTYRAC 3 cut(s) 478, 740, 1277
HindII GTYRAC 3 cut(s) 478, 740, 1277
HindIII AAGCTT 1 cut(s) 1066
HinfI GANTC 3 cut(s) 48, 1018, 1108
HpaII CCGG 3 cut(s) 41, 849, 937
HphI GGTGA 3 cut(s) 407, 1133, 1312
Hpy166II GTNNAC 4 cut(s) 478, 740, 1103, 1277
Hpy188I TCNGA 5 cut(s) 217, 567, 862, 1113, 1346
Hpy188III TCNNGA 8 cut(s) 33, 59, 95, 458, 544, 683, 849, 1303
Hpy8I GTNNAC 4 cut(s) 478, 740, 1103, 1277
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 3 cut(s) 1142, 1224, 1306
HpyCH4III ACNGT 4 cut(s) 563, 737, 844, 1210
HpyCH4IV ACGT 5 cut(s) 115, 892, 1099, 1191, 1349
HpyCH4V TGCA 7 cut(s) 132, 597, 666, 766, 875, 1042, 1061
HpyF10VI GCNNNNNNNGC 1 cut(s) 175
HpyF3I CTNAG 6 cut(s) 9, 216, 815, 983, 1049, 1294
HpySE526I ACGT 5 cut(s) 115, 892, 1099, 1191, 1349
Hsp92II CATG 6 cut(s) 320, 509, 670, 733, 770, 786
HspAI GCGC 2 cut(s) 176, 409
Kpn2I TCCGGA 1 cut(s) 848
KroI GCCGGC 1 cut(s) 936
KroNI GCCGGC 1 cut(s) 938
Ksp22I TGATCA 4 cut(s) 322, 352, 802, 862
Kzo9I GATC 9 cut(s) 4, 322, 352, 501, 679, 802, 862, 884, 950
LmnI GCTCC 1 cut(s) 43
Lsp1109I GCAGC 1 cut(s) 153
LweI GCATC 4 cut(s) 292, 676, 1048, 1070
MaeI CTAG 3 cut(s) 170, 228, 1266
MaeII ACGT 5 cut(s) 115, 892, 1099, 1191, 1349
MaeIII GTNAC 3 cut(s) 671, 778, 917
MalI GATC 9 cut(s) 6, 324, 354, 503, 681, 804, 864, 886, 952
MboI GATC 9 cut(s) 4, 322, 352, 501, 679, 802, 862, 884, 950
MboII GAAGA 6 cut(s) 25, 76, 94, 379, 816, 1321
MfeI CAATTG 1 cut(s) 1251
MflI RGATCY 2 cut(s) 4, 501
MhlI GDGCHC 4 cut(s) 40, 239, 337, 647
MluCI AATT 2 cut(s) 878, 1251
MlyI GAGTC 1 cut(s) 1027
MmeI TCCRAC 2 cut(s) 421, 1177
MnlI CCTC 7 cut(s) 9, 259, 597, 634, 937, 1258, 1290
Mph1103I ATGCAT 4 cut(s) 599, 668, 768, 1063
MroI TCCGGA 1 cut(s) 848
MroNI GCCGGC 1 cut(s) 936
MroXI GAANNNNTTC 1 cut(s) 1234
MseI TTAA 2 cut(s) 759, 881
MslI CAYNNNNRTG 1 cut(s) 787
MspA1I CMGCKG 1 cut(s) 868
MspI CCGG 3 cut(s) 41, 849, 937
MspR9I CCNGG 1 cut(s) 110
MunI CAATTG 1 cut(s) 1251
Mva1269I GAATGC 1 cut(s) 1260
MvaI CCWGG 1 cut(s) 110
MwoI GCNNNNNNNGC 1 cut(s) 175
NaeI GCCGGC 1 cut(s) 938
NdeI CATATG 1 cut(s) 599
NdeII GATC 9 cut(s) 4, 322, 352, 501, 679, 802, 862, 884, 950
NgoMIV GCCGGC 1 cut(s) 936
NlaIII CATG 6 cut(s) 320, 509, 670, 733, 770, 786
NlaIV GGNNCC 5 cut(s) 6, 236, 646, 980, 1207
NmuCI GTSAC 1 cut(s) 778
NsiI ATGCAT 4 cut(s) 599, 668, 768, 1063
NspI RCATGY 4 cut(s) 670, 733, 770, 786
PaeI GCATGC 2 cut(s) 670, 770
PciI ACATGT 1 cut(s) 729
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PctI GAATGC 1 cut(s) 1260
PdiI GCCGGC 1 cut(s) 938
PdmI GAANNNNTTC 1 cut(s) 1234
PfeI GAWTC 2 cut(s) 48, 1108
PflFI GACNNNGTC 1 cut(s) 464
PkrI GCNGC 2 cut(s) 168, 282
PleI GAGTC 1 cut(s) 1026
PpsI GAGTC 1 cut(s) 1026
Ppu21I YACGTR 1 cut(s) 1100
PpuMI RGGWCCY 1 cut(s) 979
PscI ACATGT 1 cut(s) 729
Psp124BI GAGCTC 2 cut(s) 40, 337
Psp5II RGGWCCY 1 cut(s) 979
Psp6I CCWGG 1 cut(s) 108
PspGI CCWGG 1 cut(s) 108
PspN4I GGNNCC 5 cut(s) 6, 236, 646, 980, 1207
PspPI GGNCC 2 cut(s) 773, 979
PspPPI RGGWCCY 1 cut(s) 979
PsuI RGATCY 2 cut(s) 4, 501
PsyI GACNNNGTC 1 cut(s) 464
PvuII CAGCTG 1 cut(s) 868
RsaI GTAC 2 cut(s) 734, 1340
RsaNI GTAC 2 cut(s) 733, 1339
RseI CAYNNNNRTG 1 cut(s) 787
SacI GAGCTC 2 cut(s) 40, 337
SalI GTCGAC 1 cut(s) 1275
SaqAI TTAA 2 cut(s) 759, 881
SatI GCNGC 2 cut(s) 167, 281
Sau3AI GATC 9 cut(s) 4, 322, 352, 501, 679, 802, 862, 884, 950
Sau96I GGNCC 2 cut(s) 773, 979
SchI GAGTC 1 cut(s) 1027
ScrFI CCNGG 1 cut(s) 110
SduI GDGCHC 4 cut(s) 40, 239, 337, 647
SfaNI GCATC 4 cut(s) 292, 676, 1048, 1070
SinI GGWCC 2 cut(s) 773, 979
SmiMI CAYNNNNRTG 1 cut(s) 787
SmlI CTYRAG 1 cut(s) 31
SmoI CTYRAG 1 cut(s) 31
SnaBI TACGTA 1 cut(s) 1100
SphI GCATGC 2 cut(s) 670, 770
Sse9I AATT 2 cut(s) 878, 1251
SsiI CCGC 4 cut(s) 280, 607, 819, 1298
SspMI CTAG 3 cut(s) 170, 228, 1266
SstI GAGCTC 2 cut(s) 40, 337
StyD4I CCNGG 1 cut(s) 108
TaaI ACNGT 4 cut(s) 563, 737, 844, 1210
TaiI ACGT 5 cut(s) 118, 895, 1102, 1194, 1352
TaqI TCGA 5 cut(s) 60, 96, 105, 887, 1276
TasI AATT 2 cut(s) 878, 1251
TatI WGTACW 2 cut(s) 732, 1338
TauI GCSGC 1 cut(s) 283
TfiI GAWTC 2 cut(s) 48, 1108
Tru1I TTAA 2 cut(s) 759, 881
Tru9I TTAA 2 cut(s) 759, 881
TscAI CASTG 4 cut(s) 332, 362, 783, 847
TseFI GTSAC 1 cut(s) 778
TseI GCWGC 1 cut(s) 166
Tsp45I GTSAC 1 cut(s) 778
TspDTI ATGAA 2 cut(s) 305, 1322
TspGWI ACGGA 1 cut(s) 112
TspRI CASTG 4 cut(s) 332, 362, 783, 847
Tth111I GACNNNGTC 1 cut(s) 464
VpaK11BI GGWCC 2 cut(s) 773, 979
XceI RCATGY 4 cut(s) 670, 733, 770, 786
XmiI GTMKAC 2 cut(s) 1102, 1276
XmnI GAANNNNTTC 1 cut(s) 1234
XspI CTAG 3 cut(s) 170, 228, 1266
Zsp2I ATGCAT 4 cut(s) 599, 668, 768, 1063
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.