Rh1CG031300

F-box WD-40 repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0004801
Physical Location & Seq
Forward (+)
6214716 .. 6220046
5331 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG031300.1

Sequence Viewer

Length: 1299 bp
ATGGACCCCAAGAAGAGGCCAACCAGAAGTTCAAGAGCTCCGGCCAGAATCCAATCTCTCGACCACGACATCCTCTGTATCATCTTCTCTTTTCTCGACTTCTTCGACCTCGCACGTTGTTCCGTCGTCTGCAAAACCTGGAGTGGTATTATCAACAAGTCAAAGTTGCTAGAAGCGCTTTACTACAAGAAGTGGCAGATGGGTCATGTGGGTTCTGAGAATAGGTCCAGTGGGTGCCCAGAGAAATCTTTGAATGTTTATTTGGAGGAGATGGCTCTCCAGCATCATAGACTGGCTTTACAGCAAGGTCGGGTTCATGTTGATCACTGGAGAGCTCACTCTGTTGGGGTTGATCAGTGCCGAAAGAAGATGGGTTTGCTTCTTACTGGCATTGGTGATAAGGTTATGCGCCTTTGGTCATTGGAAAGCTATAAATGTGTGGGAGAATATCCCATTCCAGACAAACTCCCTTTAGTTGACTTTGATTTTGATGAGAGCAAGATTGTTGGTTTGGTCGGCAGTCGTATATGCATATGGAGGCGGAATGGGATAAGAAGTATATTCCCCTCGCGTGAGGGCACCTTTCCAAAGGGCTTATGCATGCGTTACTGCGATCCTGATGCTGTGGTTGGCTGTGTGGATGGGACAGCTCGTGTATTTGACATGTACAGTCAACAATGTTCTCAAATCGTTAAAATGCATGCTGGTGCAGTGACATGCTTGTGTTTGAGTGATGATCAGTTGATACTTAGCGGTTCTTCTCTTGGCAGTATCACGGTGTCTAATCTTTTATCTGATCAGCGGGTTGCAGAATTAAGACTGACACATTCCACAGGCATGAGGACTTTGTGTTTCAACCCCTCATCTCACCGGCTGTTCGCTGGATCAACTGCTGGATATGTATTTTGTTGGGACCTCAGGACAAGGAGATGTTTATGGGAGACACGAGCGAGTCCAAATGAGGTATATTCAATGCAACACCTGAGAAATGATGCATCAAGCTTGGTGGTTGGTGGAATAGATGGTGTGCTACGTATACTGAATCAGAACACAGGTGAAGTAGTTTCAAGATTTGTTTTGGAAGGCAACTTATTGTCAAGTTCAAGTTCAAGTTCTCATCAACACGTAGTTGGAAGTGGCACATTCCAAAGAATGACAGGACGAAGGCTTTCACAAAATGCCTCAATTGATAGCATTCCTAGAAGCAGTCGACCTCCCATCACTTGCTTAGCGGTCGGGATGAAGAAGGTTATCACCACACACAATAATAAGTACATCAGAACATGGAAGTTCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
F-box F-box-like WD40

Protein Analysis

432

Amino Acids

48.43

Weight (kDa)

9.33

Isoelectric Point (pI)

42.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 17 - 56 1.1e-09 F-box domain
F-box-like PF12937 22 - 56 1.2e-08 F-box-like
Beta-prop_EML PF23409 133 - 300 6e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_ACSF4 PF13570 199 - 378 4.8e-13 Beta-alanine-activating enzyme, beta-propeller
WD40_WDHD1_1st PF24817 199 - 353 3.7e-09 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 208 - 346 4.8e-18 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 208 - 383 4.1e-13 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 208 - 358 1.8e-10 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 209 - 292 1.8e-06 THOC3 beta-propeller domain
WDR55 PF24796 209 - 380 2.8e-10 WDR55
Beta-prop_WDR36-Utp21_1st PF25171 209 - 331 2.2e-08 WDR36/Utp21 first beta-propeller
Beta-prop_WDR5 PF25175 212 - 364 8.2e-16 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 245 - 361 3.7e-08 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 282 - 361 2e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 234, 578
AccI GTMKAC 2 cut(s) 1036, 1210
AccII CGCG 1 cut(s) 571
AciI CCGC 4 cut(s) 541, 753, 802, 1232
AclWI GGATC 2 cut(s) 608, 892
AcoI YGGCCR 1 cut(s) 42
AfaI GTAC 2 cut(s) 668, 1274
AfeI AGCGCT 1 cut(s) 177
AfiI CCNNNNNNNGG 1 cut(s) 15
AflIII ACRYGT 2 cut(s) 663, 1123
AgsI TTSAA 8 cut(s) 33, 253, 856, 972, 1068, 1104, 1110, 1294
AjnI CCWGG 1 cut(s) 137
AjuI GAANNNNNNNTTGG 4 cut(s) 13, 45, 245, 277
AluBI AGCT 5 cut(s) 38, 335, 429, 650, 1002
AluI AGCT 5 cut(s) 38, 335, 429, 650, 1002
Alw21I GWGCWC 2 cut(s) 40, 337
Alw26I GTCTC 1 cut(s) 935
AlwI GGATC 2 cut(s) 608, 892
Aor51HI AGCGCT 1 cut(s) 177
AoxI GGCC 2 cut(s) 17, 42
ArsI GACNNNNNNTTYG 2 cut(s) 470, 502
Asp700I GAANNNNTTC 1 cut(s) 1168
AspLEI GCGC 2 cut(s) 178, 411
AspS9I GGNCC 3 cut(s) 4, 225, 913
AsuHPI GGTGA 4 cut(s) 407, 860, 1067, 1246
AvaII GGWCC 3 cut(s) 4, 225, 913
AxyI CCTNAGG 1 cut(s) 917
BaeGI GKGCMC 2 cut(s) 239, 581
BanI GGYRCC 2 cut(s) 234, 578
BanII GRGCYC 2 cut(s) 40, 337
BauI CACGAG 2 cut(s) 651, 945
Bbv12I GWGCWC 2 cut(s) 40, 337
BccI CCATC 6 cut(s) 193, 265, 364, 635, 1016, 1226
BcgI CGANNNNNNTGC 2 cut(s) 602, 636
BciT130I CCWGG 1 cut(s) 139
BclI TGATCA 4 cut(s) 322, 352, 736, 796
BcoDI GTCTC 1 cut(s) 935
BfaI CTAG 2 cut(s) 170, 1200
BfoI RGCGCY 1 cut(s) 179
BlpI GCTNAGC 1 cut(s) 1228
Bme1390I CCNGG 1 cut(s) 139
Bme18I GGWCC 3 cut(s) 4, 225, 913
BmgT120I GGNCC 3 cut(s) 4, 225, 913
BmiI GGNNCC 4 cut(s) 6, 236, 580, 914
BmrFI CCNGG 1 cut(s) 139
BmsI GCATC 4 cut(s) 292, 610, 982, 1004
BpmI CTGGAG 3 cut(s) 160, 263, 349
Bpu1102I GCTNAGC 1 cut(s) 1228
BsaAI YACGTR 2 cut(s) 1034, 1126
Bsc4I CCNNNNNNNGG 1 cut(s) 15
Bse118I RCCGGY 1 cut(s) 870
Bse1I ACTGG 4 cut(s) 228, 297, 332, 391
Bse21I CCTNAGG 1 cut(s) 917
BseBI CCWGG 1 cut(s) 139
BseGI GGATG 3 cut(s) 69, 646, 1245
BseLI CCNNNNNNNGG 1 cut(s) 15
BseMII CTCAG 3 cut(s) 207, 931, 974
BseNI ACTGG 4 cut(s) 228, 297, 332, 391
BseRI GAGGAG 1 cut(s) 281
BseSI GKGCMC 2 cut(s) 239, 581
BsgI GTGCAG 1 cut(s) 729
Bsh1236I CGCG 1 cut(s) 571
Bsh1285I CGRYCG 1 cut(s) 1236
BshFI GGCC 2 cut(s) 19, 44
BshNI GGYRCC 2 cut(s) 234, 578
BsiEI CGRYCG 1 cut(s) 1236
BsiHKAI GWGCWC 2 cut(s) 40, 337
BsiSI CCGG 2 cut(s) 41, 871
BslFI GGGAC 2 cut(s) 658, 926
BslI CCNNNNNNNGG 1 cut(s) 15
BsmAI GTCTC 1 cut(s) 935
BsmFI GGGAC 2 cut(s) 658, 926
BsmI GAATGC 1 cut(s) 1194
BsnI GGCC 2 cut(s) 19, 44
Bsp1286I GDGCHC 4 cut(s) 40, 239, 337, 581
Bsp1407I TGTACA 1 cut(s) 666
Bsp143I GATC 6 cut(s) 322, 352, 613, 736, 796, 884
Bsp1720I GCTNAGC 1 cut(s) 1228
BspACI CCGC 4 cut(s) 541, 753, 802, 1232
BspANI GGCC 2 cut(s) 19, 44
BspCNI CTCAG 3 cut(s) 208, 930, 975
BspFNI CGCG 1 cut(s) 571
BspLI GGNNCC 4 cut(s) 6, 236, 580, 914
BspPI GGATC 2 cut(s) 608, 892
BspT107I GGYRCC 2 cut(s) 234, 578
BsrFI RCCGGY 1 cut(s) 870
BsrGI TGTACA 1 cut(s) 666
BsrI ACTGG 4 cut(s) 228, 297, 332, 391
BssAI RCCGGY 1 cut(s) 870
BssMI GATC 6 cut(s) 322, 352, 613, 736, 796, 884
BssNAI GTATAC 1 cut(s) 1037
BssSI CACGAG 2 cut(s) 651, 945
Bst1107I GTATAC 1 cut(s) 1037
Bst2BI CACGAG 2 cut(s) 651, 945
Bst2UI CCWGG 1 cut(s) 139
Bst4CI ACNGT 2 cut(s) 671, 778
Bst6I CTCTTC 1 cut(s) 8
BstAUI TGTACA 1 cut(s) 666
BstBAI YACGTR 2 cut(s) 1034, 1126
BstC8I GCNNGC 2 cut(s) 602, 702
BstDEI CTNAG 5 cut(s) 216, 749, 917, 983, 1228
BstF5I GGATG 3 cut(s) 69, 646, 1245
BstFNI CGCG 1 cut(s) 571
BstH2I RGCGCY 1 cut(s) 179
BstHHI GCGC 2 cut(s) 178, 411
BstKTI GATC 6 cut(s) 325, 355, 616, 739, 799, 887
BstMAI GTCTC 1 cut(s) 935
BstMBI GATC 6 cut(s) 322, 352, 613, 736, 796, 884
BstMCI CGRYCG 1 cut(s) 1236
BstMWI GCNNNNNNNGC 1 cut(s) 175
BstNI CCWGG 1 cut(s) 139
BstNSI RCATGY 4 cut(s) 604, 667, 704, 720
BstSCI CCNGG 1 cut(s) 137
BstSLI GKGCMC 2 cut(s) 239, 581
BstSNI TACGTA 1 cut(s) 1034
BstUI CGCG 1 cut(s) 571
BstZ17I GTATAC 1 cut(s) 1037
Bsu36I CCTNAGG 1 cut(s) 917
BsuRI GGCC 2 cut(s) 19, 44
BtsCI GGATG 3 cut(s) 69, 646, 1245
BtsI GCAGTG 1 cut(s) 717
BtsIMutI CAGTG 4 cut(s) 235, 325, 362, 717
Cac8I GCNNGC 2 cut(s) 602, 702
CfoI GCGC 2 cut(s) 178, 411
Cfr10I RCCGGY 1 cut(s) 870
Cfr13I GGNCC 3 cut(s) 4, 225, 913
Csp6I GTAC 2 cut(s) 667, 1273
CspCI CAANNNNNGTGG 2 cut(s) 987, 1022
CviAII CATG 8 cut(s) 206, 317, 601, 664, 701, 717, 838, 1284
CviQI GTAC 2 cut(s) 667, 1273
DdeI CTNAG 5 cut(s) 216, 749, 917, 983, 1228
DpnI GATC 6 cut(s) 324, 354, 615, 738, 798, 886
DpnII GATC 6 cut(s) 322, 352, 613, 736, 796, 884
EaeI YGGCCR 1 cut(s) 42
Eam1104I CTCTTC 1 cut(s) 8
EarI CTCTTC 1 cut(s) 8
EciI GGCGGA 1 cut(s) 556
Ecl136II GAGCTC 2 cut(s) 38, 335
Eco105I TACGTA 1 cut(s) 1034
Eco24I GRGCYC 2 cut(s) 40, 337
Eco47I GGWCC 3 cut(s) 4, 225, 913
Eco47III AGCGCT 1 cut(s) 177
Eco53kI GAGCTC 2 cut(s) 38, 335
Eco81I CCTNAGG 1 cut(s) 917
EcoICRI GAGCTC 2 cut(s) 38, 335
EcoO109I RGGNCCY 1 cut(s) 913
EcoRII CCWGG 1 cut(s) 137
EcoT22I ATGCAT 4 cut(s) 533, 602, 702, 997
EcoT38I GRGCYC 2 cut(s) 40, 337
FaeI CATG 8 cut(s) 209, 320, 604, 667, 704, 720, 841, 1287
FaqI GGGAC 2 cut(s) 658, 926
FatI CATG 8 cut(s) 205, 316, 600, 663, 700, 716, 837, 1283
FauI CCCGC 1 cut(s) 795
FauNDI CATATG 1 cut(s) 533
FbaI TGATCA 4 cut(s) 322, 352, 736, 796
FblI GTMKAC 2 cut(s) 1036, 1210
FokI GGATG 3 cut(s) 56, 653, 1252
FriOI GRGCYC 2 cut(s) 40, 337
FspBI CTAG 2 cut(s) 170, 1200
GlaI GCGC 2 cut(s) 177, 410
GsuI CTGGAG 3 cut(s) 160, 263, 349
HaeII RGCGCY 1 cut(s) 179
HaeIII GGCC 2 cut(s) 19, 44
HapII CCGG 2 cut(s) 41, 871
HhaI GCGC 2 cut(s) 178, 411
Hin1II CATG 8 cut(s) 209, 320, 604, 667, 704, 720, 841, 1287
Hin6I GCGC 2 cut(s) 176, 409
HinP1I GCGC 2 cut(s) 176, 409
HincII GTYRAC 3 cut(s) 478, 674, 1211
HindII GTYRAC 3 cut(s) 478, 674, 1211
HindIII AAGCTT 1 cut(s) 1000
HinfI GANTC 3 cut(s) 48, 952, 1042
HpaII CCGG 2 cut(s) 41, 871
HphI GGTGA 4 cut(s) 407, 860, 1067, 1246
Hpy166II GTNNAC 4 cut(s) 478, 674, 1037, 1211
Hpy188I TCNGA 4 cut(s) 217, 796, 1047, 1280
Hpy188III TCNNGA 8 cut(s) 33, 59, 95, 458, 617, 919, 1068, 1237
Hpy8I GTNNAC 4 cut(s) 478, 674, 1037, 1211
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 3 cut(s) 1076, 1158, 1240
HpyCH4III ACNGT 2 cut(s) 671, 778
HpyCH4IV ACGT 3 cut(s) 115, 1033, 1125
HpyCH4V TGCA 8 cut(s) 132, 531, 600, 700, 710, 809, 976, 995
HpyF10VI GCNNNNNNNGC 1 cut(s) 175
HpyF3I CTNAG 5 cut(s) 216, 749, 917, 983, 1228
HpySE526I ACGT 3 cut(s) 115, 1033, 1125
Hsp92II CATG 8 cut(s) 209, 320, 604, 667, 704, 720, 841, 1287
HspAI GCGC 2 cut(s) 176, 409
Ksp22I TGATCA 4 cut(s) 322, 352, 736, 796
Kzo9I GATC 6 cut(s) 322, 352, 613, 736, 796, 884
LmnI GCTCC 1 cut(s) 43
LweI GCATC 4 cut(s) 292, 610, 982, 1004
MaeI CTAG 2 cut(s) 170, 1200
MaeII ACGT 3 cut(s) 115, 1033, 1125
MaeIII GTNAC 2 cut(s) 605, 712
MalI GATC 6 cut(s) 324, 354, 615, 738, 798, 886
MboI GATC 6 cut(s) 322, 352, 613, 736, 796, 884
MboII GAAGA 6 cut(s) 25, 76, 94, 379, 750, 1255
MfeI CAATTG 1 cut(s) 1185
MhlI GDGCHC 4 cut(s) 40, 239, 337, 581
MluCI AATT 2 cut(s) 812, 1185
MlyI GAGTC 1 cut(s) 961
MmeI TCCRAC 1 cut(s) 1111
Mph1103I ATGCAT 4 cut(s) 533, 602, 702, 997
MroXI GAANNNNTTC 1 cut(s) 1168
MseI TTAA 2 cut(s) 693, 815
MslI CAYNNNNRTG 3 cut(s) 705, 721, 836
MspA1I CMGCKG 1 cut(s) 802
MspI CCGG 2 cut(s) 41, 871
MspR9I CCNGG 1 cut(s) 139
MunI CAATTG 1 cut(s) 1185
Mva1269I GAATGC 1 cut(s) 1194
MvaI CCWGG 1 cut(s) 139
MvnI CGCG 1 cut(s) 571
MwoI GCNNNNNNNGC 1 cut(s) 175
NdeI CATATG 1 cut(s) 533
NdeII GATC 6 cut(s) 322, 352, 613, 736, 796, 884
NlaIII CATG 8 cut(s) 209, 320, 604, 667, 704, 720, 841, 1287
NlaIV GGNNCC 4 cut(s) 6, 236, 580, 914
NmuCI GTSAC 1 cut(s) 712
NsiI ATGCAT 4 cut(s) 533, 602, 702, 997
NspI RCATGY 4 cut(s) 604, 667, 704, 720
PaeI GCATGC 2 cut(s) 604, 704
PciI ACATGT 1 cut(s) 663
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PctI GAATGC 1 cut(s) 1194
PdmI GAANNNNTTC 1 cut(s) 1168
PfeI GAWTC 2 cut(s) 48, 1042
PleI GAGTC 1 cut(s) 960
PpsI GAGTC 1 cut(s) 960
Ppu21I YACGTR 2 cut(s) 1034, 1126
PpuMI RGGWCCY 1 cut(s) 913
PscI ACATGT 1 cut(s) 663
Psp124BI GAGCTC 2 cut(s) 40, 337
Psp5II RGGWCCY 1 cut(s) 913
Psp6I CCWGG 1 cut(s) 137
PspGI CCWGG 1 cut(s) 137
PspN4I GGNNCC 4 cut(s) 6, 236, 580, 914
PspPI GGNCC 3 cut(s) 4, 225, 913
PspPPI RGGWCCY 1 cut(s) 913
RsaI GTAC 2 cut(s) 668, 1274
RsaNI GTAC 2 cut(s) 667, 1273
RseI CAYNNNNRTG 3 cut(s) 705, 721, 836
SacI GAGCTC 2 cut(s) 40, 337
SalI GTCGAC 1 cut(s) 1209
SaqAI TTAA 2 cut(s) 693, 815
Sau3AI GATC 6 cut(s) 322, 352, 613, 736, 796, 884
Sau96I GGNCC 3 cut(s) 4, 225, 913
SchI GAGTC 1 cut(s) 961
ScrFI CCNGG 1 cut(s) 139
SduI GDGCHC 4 cut(s) 40, 239, 337, 581
SfaNI GCATC 4 cut(s) 292, 610, 982, 1004
SinI GGWCC 3 cut(s) 4, 225, 913
SmiMI CAYNNNNRTG 3 cut(s) 705, 721, 836
SnaBI TACGTA 1 cut(s) 1034
SphI GCATGC 2 cut(s) 604, 704
Sse9I AATT 2 cut(s) 812, 1185
SsiI CCGC 4 cut(s) 541, 753, 802, 1232
SspMI CTAG 2 cut(s) 170, 1200
SstI GAGCTC 2 cut(s) 40, 337
StyD4I CCNGG 1 cut(s) 137
TaaI ACNGT 2 cut(s) 671, 778
TaiI ACGT 3 cut(s) 118, 1036, 1128
TaqI TCGA 4 cut(s) 60, 96, 105, 1210
TasI AATT 2 cut(s) 812, 1185
TatI WGTACW 2 cut(s) 666, 1272
TfiI GAWTC 2 cut(s) 48, 1042
Tru1I TTAA 2 cut(s) 693, 815
Tru9I TTAA 2 cut(s) 693, 815
TscAI CASTG 4 cut(s) 235, 332, 362, 717
TseFI GTSAC 1 cut(s) 712
Tsp45I GTSAC 1 cut(s) 712
TspDTI ATGAA 2 cut(s) 305, 1256
TspGWI ACGGA 1 cut(s) 112
TspRI CASTG 4 cut(s) 235, 332, 362, 717
VpaK11BI GGWCC 3 cut(s) 4, 225, 913
XceI RCATGY 4 cut(s) 604, 667, 704, 720
XmiI GTMKAC 2 cut(s) 1036, 1210
XmnI GAANNNNTTC 1 cut(s) 1168
XspI CTAG 2 cut(s) 170, 1200
Zsp2I ATGCAT 4 cut(s) 533, 602, 702, 997
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.