RLG00000030542

F-box WD-40 repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004801
Physical Location & Seq
Reverse (-)
63249914 .. 63255225
5312 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030542

Sequence Viewer

Length: 1314 bp
ATGGATCCCAGTCGCGTCGGCAGCAAGAAGAGGCCAACCAGAAGCTCGAAAGCTCCGGTCAGAATCCAATCTCTCGACCACGACATCCTATGTATCATCTTCTCTTTTCTCGACTTCTTCGACCTCGCACGTTGTTCCGTCGTCTGCAAAAGCTGGAGTGGTATTATCAACAAGTCAAAGTTGCTAGAAGCGCTGTACTACAAGAAGTGGCAGATGGGTCTTGTGGGTTCTGAGAATAGGTCTAGTGGGTGCCCAGAGAAATCTTTGAATGTTTATTTGGAGGAGATGGCTCTGCAGCATCATAGACTGGCTTTACAGCAAGGTCGGGTTCATGTTGATCACTGGAGAGCTCACTCTGTTGGGGTTGATCAGTGCCGAAAGAAGATGGGTTTGCTTCTTACTGGCATTGGTGATAAGGTTATGCGCCTTTGGTCATTGGAAAGCTATAAATGTGTGGGAGAATATCCCATTCCAAACAAAGTCCCTTTAGTTGACTTTGATTTTGATGAGAGCAAGATTGTTGGTTTGGTTGGCAGTCGTATATGCATATGGAGGCGGAATGGGATAAGAAGTATATTCCCCTCGCGTGAGGGCACCTTTCCAAAGGGCTTGTGCATGCGTTACTGCGATCCTGATGCTGTGGTTGGCTGTGTGGATGGGACAGCTCGTGTATTTGACATGTACAGTCAACAATGTTCTCAAATCGTTAAAATGCATGCTGGTCCAGTGACATGCTTATGTTTGAGTGATGATCAGTTGATACTTAGCGGTTCTTCTGTTGGCAGTATCACGGTGTCCGGACTTTTATCTGATCAGCTGGTTACAGAATTAAGATCGACAGATTCCACAGGAATAAGGACTTTGTGTTTCAACCCCTCATCTCACCGGCTGTTCGCTGGATCAACTGCTGGATATGTATTTTGTTGGGACCTCAGGACAAGGAGATGTTTATGGGAGACACGAGTGAGTCCGAATGAGGTATATTCAATGCAACACCTGAGAAATGATGCATCAAGCTTGGTGGTTGGTGGAATAGATGGTGTGCTACGTATACTGAATCAGAACACAGGTGAAGTAGTTTCAAGATTTGTTTTGGAAGGCAACTTATTGTCAAGTTCAAGTTCAAGTTCTCATCAAAACGTAGTTGGAAGTGGCACTGTCCAAAGAATGATAGGACGAAGGCTTTCACAAAATGCCTCAATTGATAGCATTCCTAGAAGCAGTCGACCTCCCATCACTTGCTTAGCGGTCGGGATGAAGAAGGTTATCACCACACACAATAATAAGTACATCAGAACGTGGAAGTTCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
F-box F-box-like WD40

Protein Analysis

438

Amino Acids

48.71

Weight (kDa)

9.32

Isoelectric Point (pI)

45.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 22 - 61 1.3e-09 F-box domain
F-box-like PF12937 27 - 61 6.7e-09 F-box-like
Beta-prop_EML PF23409 138 - 303 1.8e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_HPS5 PF23756 202 - 316 8.5e-06 HPS5 beta-propeller
Beta-prop_ACSF4 PF13570 204 - 382 6.3e-13 Beta-alanine-activating enzyme, beta-propeller
WD40_WDHD1_1st PF24817 204 - 416 5.6e-09 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 213 - 351 2.5e-16 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 213 - 363 1.2e-09 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 214 - 385 5.3e-12 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 214 - 321 1.1e-06 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_2nd PF25172 215 - 354 5.5e-08 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 217 - 378 2.9e-14 WDR5 beta-propeller domain
WDR55 PF24796 219 - 366 8.9e-11 WDR55
Beta-prop_THOC3 PF25174 252 - 365 5.7e-07 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 287 - 382 7.8e-07 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 249, 593
AccI GTMKAC 2 cut(s) 1051, 1225
AccII CGCG 2 cut(s) 15, 586
AccIII TCCGGA 1 cut(s) 797
AciI CCGC 3 cut(s) 556, 768, 1247
AclWI GGATC 3 cut(s) 12, 623, 907
AfaI GTAC 3 cut(s) 197, 683, 1289
AfeI AGCGCT 1 cut(s) 192
AflIII ACRYGT 1 cut(s) 678
AgsI TTSAA 7 cut(s) 268, 871, 987, 1083, 1119, 1125, 1309
AjuI GAANNNNNNNTTGG 2 cut(s) 260, 292
AluBI AGCT 8 cut(s) 45, 53, 153, 350, 444, 665, 817, 1017
AluI AGCT 8 cut(s) 45, 53, 153, 350, 444, 665, 817, 1017
Alw21I GWGCWC 1 cut(s) 352
Alw26I GTCTC 1 cut(s) 950
AlwI GGATC 3 cut(s) 12, 623, 907
Aor13HI TCCGGA 1 cut(s) 797
Aor51HI AGCGCT 1 cut(s) 192
AoxI GGCC 1 cut(s) 32
ApeKI GCWGC 2 cut(s) 21, 295
ArsI GACNNNNNNTTYG 2 cut(s) 485, 517
Asp700I GAANNNNTTC 1 cut(s) 1183
AspLEI GCGC 2 cut(s) 193, 426
AspS9I GGNCC 2 cut(s) 722, 928
AsuHPI GGTGA 4 cut(s) 422, 875, 1082, 1261
AvaII GGWCC 2 cut(s) 722, 928
AxyI CCTNAGG 1 cut(s) 932
BaeGI GKGCMC 2 cut(s) 254, 596
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 2 cut(s) 249, 593
BanII GRGCYC 1 cut(s) 352
BauI CACGAG 2 cut(s) 666, 960
Bbv12I GWGCWC 1 cut(s) 352
BbvI GCAGC 2 cut(s) 33, 307
BccI CCATC 6 cut(s) 208, 280, 379, 650, 1031, 1241
BcgI CGANNNNNNTGC 2 cut(s) 617, 651
BclI TGATCA 4 cut(s) 337, 367, 751, 811
BcoDI GTCTC 1 cut(s) 950
BfaI CTAG 3 cut(s) 185, 243, 1215
BfmI CTRYAG 1 cut(s) 293
BfoI RGCGCY 1 cut(s) 194
BisI GCNGC 2 cut(s) 22, 296
BlpI GCTNAGC 1 cut(s) 1243
BlsI GCNGC 2 cut(s) 23, 297
Bme18I GGWCC 2 cut(s) 722, 928
BmgT120I GGNCC 2 cut(s) 722, 928
BmiI GGNNCC 4 cut(s) 6, 251, 595, 929
BmrI ACTGGG 1 cut(s) 3
BmsI GCATC 4 cut(s) 307, 625, 997, 1019
BmuI ACTGGG 1 cut(s) 3
BpmI CTGGAG 2 cut(s) 175, 364
Bpu1102I GCTNAGC 1 cut(s) 1243
BsaAI YACGTR 1 cut(s) 1049
BsaWI WCCGGW 2 cut(s) 55, 797
Bse118I RCCGGY 1 cut(s) 885
Bse1I ACTGG 5 cut(s) 9, 312, 347, 406, 725
Bse21I CCTNAGG 1 cut(s) 932
BseAI TCCGGA 1 cut(s) 797
BseGI GGATG 3 cut(s) 84, 661, 1260
BseMII CTCAG 3 cut(s) 222, 946, 989
BseNI ACTGG 5 cut(s) 9, 312, 347, 406, 725
BseRI GAGGAG 1 cut(s) 296
BseSI GKGCMC 2 cut(s) 254, 596
BseXI GCAGC 2 cut(s) 33, 307
Bsh1236I CGCG 2 cut(s) 15, 586
Bsh1285I CGRYCG 1 cut(s) 1251
BshFI GGCC 1 cut(s) 34
BshNI GGYRCC 2 cut(s) 249, 593
BsiEI CGRYCG 1 cut(s) 1251
BsiHKAI GWGCWC 1 cut(s) 352
BsiSI CCGG 3 cut(s) 56, 798, 886
BslFI GGGAC 3 cut(s) 467, 673, 941
BsmAI GTCTC 1 cut(s) 950
BsmFI GGGAC 3 cut(s) 467, 673, 941
BsmI GAATGC 1 cut(s) 1209
BsnI GGCC 1 cut(s) 34
Bsp1286I GDGCHC 3 cut(s) 254, 352, 596
Bsp13I TCCGGA 1 cut(s) 797
Bsp1407I TGTACA 1 cut(s) 681
Bsp143I GATC 8 cut(s) 4, 337, 367, 628, 751, 811, 833, 899
Bsp1720I GCTNAGC 1 cut(s) 1243
BspACI CCGC 3 cut(s) 556, 768, 1247
BspANI GGCC 1 cut(s) 34
BspCNI CTCAG 3 cut(s) 223, 945, 990
BspEI TCCGGA 1 cut(s) 797
BspFNI CGCG 2 cut(s) 15, 586
BspLI GGNNCC 4 cut(s) 6, 251, 595, 929
BspMAI CTGCAG 1 cut(s) 297
BspPI GGATC 3 cut(s) 12, 623, 907
BspT107I GGYRCC 2 cut(s) 249, 593
BsrFI RCCGGY 1 cut(s) 885
BsrGI TGTACA 1 cut(s) 681
BsrI ACTGG 5 cut(s) 9, 312, 347, 406, 725
BssAI RCCGGY 1 cut(s) 885
BssMI GATC 8 cut(s) 4, 337, 367, 628, 751, 811, 833, 899
BssNAI GTATAC 1 cut(s) 1052
BssSI CACGAG 2 cut(s) 666, 960
Bst1107I GTATAC 1 cut(s) 1052
Bst2BI CACGAG 2 cut(s) 666, 960
Bst4CI ACNGT 3 cut(s) 686, 793, 1159
Bst6I CTCTTC 1 cut(s) 23
BstAUI TGTACA 1 cut(s) 681
BstBAI YACGTR 1 cut(s) 1049
BstC8I GCNNGC 2 cut(s) 617, 717
BstDEI CTNAG 5 cut(s) 231, 764, 932, 998, 1243
BstF5I GGATG 3 cut(s) 84, 661, 1260
BstFNI CGCG 2 cut(s) 15, 586
BstH2I RGCGCY 1 cut(s) 194
BstHHI GCGC 2 cut(s) 193, 426
BstKTI GATC 8 cut(s) 7, 340, 370, 631, 754, 814, 836, 902
BstMAI GTCTC 1 cut(s) 950
BstMBI GATC 8 cut(s) 4, 337, 367, 628, 751, 811, 833, 899
BstMCI CGRYCG 1 cut(s) 1251
BstMWI GCNNNNNNNGC 2 cut(s) 21, 190
BstNSI RCATGY 4 cut(s) 619, 682, 719, 735
BstSFI CTRYAG 1 cut(s) 293
BstSLI GKGCMC 2 cut(s) 254, 596
BstSNI TACGTA 1 cut(s) 1049
BstUI CGCG 2 cut(s) 15, 586
BstV1I GCAGC 2 cut(s) 33, 307
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BstZ17I GTATAC 1 cut(s) 1052
Bsu36I CCTNAGG 1 cut(s) 932
BsuRI GGCC 1 cut(s) 34
BtsCI GGATG 3 cut(s) 84, 661, 1260
BtsIMutI CAGTG 4 cut(s) 340, 377, 732, 1155
Cac8I GCNNGC 2 cut(s) 617, 717
CfoI GCGC 2 cut(s) 193, 426
Cfr10I RCCGGY 1 cut(s) 885
Cfr13I GGNCC 2 cut(s) 722, 928
CseI GACGC 1 cut(s) 4
Csp6I GTAC 3 cut(s) 196, 682, 1288
CspCI CAANNNNNGTGG 2 cut(s) 1002, 1037
CviAII CATG 5 cut(s) 332, 616, 679, 716, 732
CviQI GTAC 3 cut(s) 196, 682, 1288
DdeI CTNAG 5 cut(s) 231, 764, 932, 998, 1243
DpnI GATC 8 cut(s) 6, 339, 369, 630, 753, 813, 835, 901
DpnII GATC 8 cut(s) 4, 337, 367, 628, 751, 811, 833, 899
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
EciI GGCGGA 1 cut(s) 571
Ecl136II GAGCTC 1 cut(s) 350
Eco105I TACGTA 1 cut(s) 1049
Eco24I GRGCYC 1 cut(s) 352
Eco47I GGWCC 2 cut(s) 722, 928
Eco47III AGCGCT 1 cut(s) 192
Eco53kI GAGCTC 1 cut(s) 350
Eco81I CCTNAGG 1 cut(s) 932
EcoICRI GAGCTC 1 cut(s) 350
EcoO109I RGGNCCY 1 cut(s) 928
EcoT22I ATGCAT 3 cut(s) 548, 717, 1012
EcoT38I GRGCYC 1 cut(s) 352
FaeI CATG 5 cut(s) 335, 619, 682, 719, 735
FaqI GGGAC 3 cut(s) 467, 673, 941
FatI CATG 5 cut(s) 331, 615, 678, 715, 731
FauNDI CATATG 1 cut(s) 548
FbaI TGATCA 4 cut(s) 337, 367, 751, 811
FblI GTMKAC 2 cut(s) 1051, 1225
Fnu4HI GCNGC 2 cut(s) 22, 296
FokI GGATG 3 cut(s) 71, 668, 1267
FriOI GRGCYC 1 cut(s) 352
Fsp4HI GCNGC 2 cut(s) 22, 296
FspBI CTAG 3 cut(s) 185, 243, 1215
GlaI GCGC 2 cut(s) 192, 425
GluI GCNGC 2 cut(s) 22, 296
GsuI CTGGAG 2 cut(s) 175, 364
HaeII RGCGCY 1 cut(s) 194
HaeIII GGCC 1 cut(s) 34
HapII CCGG 3 cut(s) 56, 798, 886
HgaI GACGC 1 cut(s) 4
HhaI GCGC 2 cut(s) 193, 426
Hin1II CATG 5 cut(s) 335, 619, 682, 719, 735
Hin6I GCGC 2 cut(s) 191, 424
HinP1I GCGC 2 cut(s) 191, 424
HincII GTYRAC 3 cut(s) 493, 689, 1226
HindII GTYRAC 3 cut(s) 493, 689, 1226
HindIII AAGCTT 1 cut(s) 1015
HinfI GANTC 4 cut(s) 63, 842, 967, 1057
HpaII CCGG 3 cut(s) 56, 798, 886
HphI GGTGA 4 cut(s) 422, 875, 1082, 1261
Hpy166II GTNNAC 4 cut(s) 493, 689, 1052, 1226
Hpy188I TCNGA 6 cut(s) 62, 232, 811, 972, 1062, 1295
Hpy188III TCNNGA 7 cut(s) 74, 110, 632, 798, 934, 1083, 1252
Hpy8I GTNNAC 4 cut(s) 493, 689, 1052, 1226
Hpy99I CGWCG 2 cut(s) 20, 143
HpyAV CCTTC 3 cut(s) 1091, 1173, 1255
HpyCH4III ACNGT 3 cut(s) 686, 793, 1159
HpyCH4IV ACGT 4 cut(s) 130, 1048, 1140, 1298
HpyCH4V TGCA 7 cut(s) 147, 295, 546, 615, 715, 991, 1010
HpyF10VI GCNNNNNNNGC 2 cut(s) 21, 190
HpyF3I CTNAG 5 cut(s) 231, 764, 932, 998, 1243
HpySE526I ACGT 4 cut(s) 130, 1048, 1140, 1298
Hsp92II CATG 5 cut(s) 335, 619, 682, 719, 735
HspAI GCGC 2 cut(s) 191, 424
Kpn2I TCCGGA 1 cut(s) 797
Ksp22I TGATCA 4 cut(s) 337, 367, 751, 811
Kzo9I GATC 8 cut(s) 4, 337, 367, 628, 751, 811, 833, 899
LmnI GCTCC 1 cut(s) 58
Lsp1109I GCAGC 2 cut(s) 33, 307
LweI GCATC 4 cut(s) 307, 625, 997, 1019
MaeI CTAG 3 cut(s) 185, 243, 1215
MaeII ACGT 4 cut(s) 130, 1048, 1140, 1298
MaeIII GTNAC 3 cut(s) 620, 727, 820
MalI GATC 8 cut(s) 6, 339, 369, 630, 753, 813, 835, 901
MboI GATC 8 cut(s) 4, 337, 367, 628, 751, 811, 833, 899
MboII GAAGA 6 cut(s) 40, 91, 109, 394, 765, 1270
MfeI CAATTG 1 cut(s) 1200
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 3 cut(s) 254, 352, 596
MluCI AATT 2 cut(s) 827, 1200
MlyI GAGTC 1 cut(s) 976
MmeI TCCRAC 1 cut(s) 1126
Mph1103I ATGCAT 3 cut(s) 548, 717, 1012
MroI TCCGGA 1 cut(s) 797
MroXI GAANNNNTTC 1 cut(s) 1183
MseI TTAA 2 cut(s) 708, 830
MslI CAYNNNNRTG 1 cut(s) 736
MspA1I CMGCKG 1 cut(s) 817
MspI CCGG 3 cut(s) 56, 798, 886
MunI CAATTG 1 cut(s) 1200
Mva1269I GAATGC 1 cut(s) 1209
MvnI CGCG 2 cut(s) 15, 586
MwoI GCNNNNNNNGC 2 cut(s) 21, 190
NdeI CATATG 1 cut(s) 548
NdeII GATC 8 cut(s) 4, 337, 367, 628, 751, 811, 833, 899
NlaIII CATG 5 cut(s) 335, 619, 682, 719, 735
NlaIV GGNNCC 4 cut(s) 6, 251, 595, 929
NmuCI GTSAC 1 cut(s) 727
NsiI ATGCAT 3 cut(s) 548, 717, 1012
NspI RCATGY 4 cut(s) 619, 682, 719, 735
PaeI GCATGC 2 cut(s) 619, 719
PciI ACATGT 1 cut(s) 678
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PctI GAATGC 1 cut(s) 1209
PdmI GAANNNNTTC 1 cut(s) 1183
PfeI GAWTC 3 cut(s) 63, 842, 1057
PkrI GCNGC 2 cut(s) 23, 297
PleI GAGTC 1 cut(s) 975
PpsI GAGTC 1 cut(s) 975
Ppu21I YACGTR 1 cut(s) 1049
PpuMI RGGWCCY 1 cut(s) 928
PscI ACATGT 1 cut(s) 678
Psp124BI GAGCTC 1 cut(s) 352
Psp5II RGGWCCY 1 cut(s) 928
PspN4I GGNNCC 4 cut(s) 6, 251, 595, 929
PspPI GGNCC 2 cut(s) 722, 928
PspPPI RGGWCCY 1 cut(s) 928
PstI CTGCAG 1 cut(s) 297
PsuI RGATCY 1 cut(s) 4
PvuII CAGCTG 1 cut(s) 817
RsaI GTAC 3 cut(s) 197, 683, 1289
RsaNI GTAC 3 cut(s) 196, 682, 1288
RseI CAYNNNNRTG 1 cut(s) 736
SacI GAGCTC 1 cut(s) 352
SalI GTCGAC 1 cut(s) 1224
SaqAI TTAA 2 cut(s) 708, 830
SatI GCNGC 2 cut(s) 22, 296
Sau3AI GATC 8 cut(s) 4, 337, 367, 628, 751, 811, 833, 899
Sau96I GGNCC 2 cut(s) 722, 928
SchI GAGTC 1 cut(s) 976
SduI GDGCHC 3 cut(s) 254, 352, 596
SfaNI GCATC 4 cut(s) 307, 625, 997, 1019
SfcI CTRYAG 1 cut(s) 293
SinI GGWCC 2 cut(s) 722, 928
SmiMI CAYNNNNRTG 1 cut(s) 736
SnaBI TACGTA 1 cut(s) 1049
SphI GCATGC 2 cut(s) 619, 719
Sse9I AATT 2 cut(s) 827, 1200
SsiI CCGC 3 cut(s) 556, 768, 1247
SspMI CTAG 3 cut(s) 185, 243, 1215
SstI GAGCTC 1 cut(s) 352
TaaI ACNGT 3 cut(s) 686, 793, 1159
TaiI ACGT 4 cut(s) 133, 1051, 1143, 1301
TaqI TCGA 6 cut(s) 47, 75, 111, 120, 836, 1225
TasI AATT 2 cut(s) 827, 1200
TatI WGTACW 3 cut(s) 195, 681, 1287
TfiI GAWTC 3 cut(s) 63, 842, 1057
Tru1I TTAA 2 cut(s) 708, 830
Tru9I TTAA 2 cut(s) 708, 830
TscAI CASTG 4 cut(s) 347, 377, 732, 1162
TseFI GTSAC 1 cut(s) 727
TseI GCWGC 2 cut(s) 21, 295
Tsp45I GTSAC 1 cut(s) 727
TspDTI ATGAA 2 cut(s) 320, 1271
TspGWI ACGGA 1 cut(s) 127
TspRI CASTG 4 cut(s) 347, 377, 732, 1162
VpaK11BI GGWCC 2 cut(s) 722, 928
XceI RCATGY 4 cut(s) 619, 682, 719, 735
XmiI GTMKAC 2 cut(s) 1051, 1225
XmnI GAANNNNTTC 1 cut(s) 1183
XspI CTAG 3 cut(s) 185, 243, 1215
Zsp2I ATGCAT 3 cut(s) 548, 717, 1012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.