FvH4_7g09930

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0006015
Physical Location & Seq
Forward (+)
9439225 .. 9445104
5880 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g09930.t4

Sequence Viewer

Length: 1431 bp
ATGGGCCCTGAATTGGAGCTGAAACGAAATTCAAATTCTGCAATGGAAGTTTCAGCTGTGAAGGAGACTGGTGTGATCCGTATAGACCCGGAAGAGACGTTTATCCCTTGTGTGAGTAACTACAAGGAAAATATTTTTAACATGGATGAATTGTGTGCGAAACAGACTACTACATTGAATAGGATCGAGAATCAGGAGATAAATATTACAGAATGTTCTGGTCCAAATGAAAACGTTCAGATAGTTGAAAATGAATGCCAGGATTTGACAGAGAGCTCGAGTTCTTTTGGTGATACAGTATCTGGGACAGAGAATGGTTCAATGTTGGATGGTGATGAAGTTGAATCGCAGTATTGTGAAAATCCGTCAGTGTCATTATATGATGGTTATTCTGATGCATTTCATACAAGGAAAAAAAAGTTGACTGCTCACTGGAGGAAATTTATACGGCCTCTTATGTGGCGCTGTAAATGGCTAGAACTGCAGATTAAGGAACTTCAGTCCCAAACCCTGAAATATGACGCAGAACTTGCAGAATATGATAAACTAAAGCAGTATGAATTTGGAGGTTACACAGCAGAAGGTTTTGATGGAAAGTCAATACCATTTTCAAGTCACATTCAAAGAAGCAAACTCATGAAAAGGAAGAAAAGAAAGAGAGTTGAAGATACTACTGATATAGTGTCATTTACGTCAAACCATACACTGTTTTCCTATTATGAAAATAAAAAGTCTGGTGCTAATGGTGGTTCCATGGAAGAGGATTGTGGTAATTTAGTGGGTAGAAGTACACATGGCATAAATGAGTTTGAGATTGAGTTCAGAGATGGTGATAGTACCTTGGAAGATATTCTTCGGAAGATTGAAGTGGTACATTCACAAGTCTGCCGGTTGAAGACCCGAATTGACAAGGTGGTGAAGGAAAATCCTGCATTGTTTGGCTCTGCTCTAAGTCCTGCTCAGAGTCCTGCTCTTGAAAGTGGGAATGAATTACTAGCGGAAACTCTACCATATGCATCTCAGCATATATCTGAGTGTAACACTGGACCTTTACTTTTGCCTAGTAGTGCAGTTTCAAGTCTTGAAGGGCTAAACCTTCTTCCTGCTAAAATGGGGAACACAGATCAGCCTTTGATTGGCACTGAACAGGAAAATGCTGTGGACAGATACCTTGTACCCAATGCATCTGCCAAGAAAGAGCCACACGATTTTGAAAACAATAATGATCAAGTCATAGAGAAGCCTCTCCTATCAAGTGCTCAGCAGAAAACTAATTTCTCTGTTCCAGCTTCCAAAACTGTGCCAAAAAAATCCAGCAAACCTAATGCAACTGAACAACCTGGTTCCACTACAAGGACTGAATTCCCTTGGAACACAAGAAATCGTGGGAAGCGTAAACCTGGTTCGTATAGGTTGAGCCGGAAATCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

477

Amino Acids

53.37

Weight (kDa)

5.63

Isoelectric Point (pI)

49.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 998
AclI AACGTT 1 cut(s) 234
AclWI GGATC 2 cut(s) 70, 191
AcsI RAATTY 5 cut(s) 28, 34, 440, 560, 1361
AcuI CTGAAG 1 cut(s) 482
AfaI GTAC 4 cut(s) 790, 838, 873, 1176
AfiI CCNNNNNNNGG 3 cut(s) 13, 1136, 1353
AjnI CCWGG 3 cut(s) 258, 1339, 1399
AluBI AGCT 4 cut(s) 19, 56, 276, 1289
AluI AGCT 4 cut(s) 19, 56, 276, 1289
Alw21I GWGCWC 2 cut(s) 278, 1261
Alw26I GTCTC 2 cut(s) 59, 89
AlwI GGATC 2 cut(s) 70, 191
AlwNI CAGNNNCTG 1 cut(s) 302
Ama87I CYCGRG 1 cut(s) 277
AoxI GGCC 2 cut(s) 4, 449
ApaI GGGCCC 1 cut(s) 8
ApoI RAATTY 5 cut(s) 28, 34, 440, 560, 1361
Asp700I GAANNNNTTC 2 cut(s) 234, 849
AspLEI GCGC 1 cut(s) 465
AspS9I GGNCC 4 cut(s) 4, 5, 221, 1046
AsuC2I CCSGG 1 cut(s) 89
AsuHPI GGTGA 4 cut(s) 302, 344, 842, 928
AvaI CYCGRG 1 cut(s) 277
AvaII GGWCC 2 cut(s) 221, 1046
BaeGI GKGCMC 1 cut(s) 8
BanII GRGCYC 2 cut(s) 8, 278
BbsI GAAGAC 1 cut(s) 902
Bbv12I GWGCWC 2 cut(s) 278, 1261
BccI CCATC 4 cut(s) 323, 377, 584, 821
BceAI ACGGC 1 cut(s) 464
BciT130I CCWGG 3 cut(s) 260, 1341, 1401
BclI TGATCA 1 cut(s) 1225
BcnI CCSGG 1 cut(s) 89
BcoDI GTCTC 2 cut(s) 59, 89
BfaI CTAG 3 cut(s) 476, 995, 1062
BfmI CTRYAG 1 cut(s) 482
BfoI RGCGCY 1 cut(s) 466
BlpI GCTNAGC 1 cut(s) 1260
Bme1390I CCNGG 4 cut(s) 89, 260, 1341, 1401
Bme18I GGWCC 2 cut(s) 221, 1046
BmeT110I CYCGRG 1 cut(s) 277
BmgT120I GGNCC 4 cut(s) 4, 5, 221, 1046
BmiI GGNNCC 3 cut(s) 6, 751, 1345
BmrFI CCNGG 4 cut(s) 89, 260, 1341, 1401
BmsI GCATC 3 cut(s) 385, 1025, 1193
BpiI GAAGAC 1 cut(s) 902
BplI GAGNNNNNCTC 2 cut(s) 955, 987
BpmI CTGGAG 1 cut(s) 454
Bpu1102I GCTNAGC 1 cut(s) 1260
BpuMI CCSGG 1 cut(s) 89
BsaJI CCNNGG 3 cut(s) 753, 840, 1367
Bsc4I CCNNNNNNNGG 3 cut(s) 13, 1136, 1353
Bse118I RCCGGY 1 cut(s) 888
Bse1I ACTGG 3 cut(s) 73, 437, 1048
Bse3DI GCAATG 1 cut(s) 48
BseBI CCWGG 3 cut(s) 260, 1341, 1401
BseDI CCNNGG 3 cut(s) 753, 840, 1367
BseGI GGATG 2 cut(s) 151, 334
BseLI CCNNNNNNNGG 3 cut(s) 13, 1136, 1353
BseMI GCAATG 1 cut(s) 48
BseMII CTCAG 4 cut(s) 974, 1023, 1034, 1274
BseNI ACTGG 3 cut(s) 73, 437, 1048
BseSI GKGCMC 1 cut(s) 8
BsgI GTGCAG 1 cut(s) 1089
BshFI GGCC 2 cut(s) 6, 451
BsiHKAI GWGCWC 2 cut(s) 278, 1261
BsiHKCI CYCGRG 1 cut(s) 277
BsiSI CCGG 3 cut(s) 89, 889, 1420
BslFI GGGAC 2 cut(s) 319, 487
BslI CCNNNNNNNGG 3 cut(s) 13, 1136, 1353
BsmAI GTCTC 2 cut(s) 59, 89
BsmBI CGTCTC 1 cut(s) 89
BsmFI GGGAC 2 cut(s) 319, 487
BsmI GAATGC 1 cut(s) 260
BsnI GGCC 2 cut(s) 6, 451
BsoBI CYCGRG 1 cut(s) 277
Bsp120I GGGCCC 1 cut(s) 4
Bsp1286I GDGCHC 3 cut(s) 8, 278, 1261
Bsp143I GATC 4 cut(s) 75, 183, 1123, 1225
Bsp1720I GCTNAGC 1 cut(s) 1260
Bsp19I CCATGG 1 cut(s) 753
BspACI CCGC 1 cut(s) 998
BspANI GGCC 2 cut(s) 6, 451
BspCNI CTCAG 4 cut(s) 973, 1024, 1033, 1273
BspHI TCATGA 1 cut(s) 636
BspLI GGNNCC 3 cut(s) 6, 751, 1345
BspMAI CTGCAG 1 cut(s) 486
BspPI GGATC 2 cut(s) 70, 191
BsrDI GCAATG 1 cut(s) 48
BsrFI RCCGGY 1 cut(s) 888
BsrI ACTGG 3 cut(s) 73, 437, 1048
BssAI RCCGGY 1 cut(s) 888
BssECI CCNNGG 3 cut(s) 753, 840, 1367
BssMI GATC 4 cut(s) 75, 183, 1123, 1225
BssT1I CCWWGG 3 cut(s) 753, 840, 1367
Bst2UI CCWGG 3 cut(s) 260, 1341, 1401
Bst4CI ACNGT 3 cut(s) 298, 708, 1300
Bst6I CTCTTC 2 cut(s) 87, 753
BstAPI GCANNNNNTGC 1 cut(s) 530
BstDEI CTNAG 5 cut(s) 950, 960, 1020, 1032, 1260
BstDSI CCRYGG 1 cut(s) 753
BstF5I GGATG 2 cut(s) 151, 334
BstH2I RGCGCY 1 cut(s) 466
BstHHI GCGC 1 cut(s) 465
BstKTI GATC 4 cut(s) 78, 186, 1126, 1228
BstMAI GTCTC 2 cut(s) 59, 89
BstMBI GATC 4 cut(s) 75, 183, 1123, 1225
BstMWI GCNNNNNNNGC 2 cut(s) 481, 530
BstNI CCWGG 3 cut(s) 260, 1341, 1401
BstSCI CCNGG 4 cut(s) 87, 258, 1339, 1399
BstSFI CTRYAG 1 cut(s) 482
BstSLI GKGCMC 1 cut(s) 8
BstV2I GAAGAC 1 cut(s) 902
BsuRI GGCC 2 cut(s) 6, 451
BtgI CCRYGG 1 cut(s) 753
BtsCI GGATG 2 cut(s) 151, 334
BtsIMutI CAGTG 5 cut(s) 375, 430, 704, 1041, 1140
CaiI CAGNNNCTG 1 cut(s) 302
CciI TCATGA 1 cut(s) 636
CfoI GCGC 1 cut(s) 465
Cfr10I RCCGGY 1 cut(s) 888
Cfr13I GGNCC 4 cut(s) 4, 5, 221, 1046
CseI GACGC 1 cut(s) 530
CsiI ACCWGGT 2 cut(s) 1339, 1399
Csp6I GTAC 4 cut(s) 789, 837, 872, 1175
CviAII CATG 4 cut(s) 142, 637, 754, 794
CviQI GTAC 4 cut(s) 789, 837, 872, 1175
DdeI CTNAG 5 cut(s) 950, 960, 1020, 1032, 1260
DpnI GATC 4 cut(s) 77, 185, 1125, 1227
DpnII GATC 4 cut(s) 75, 183, 1123, 1225
Eam1104I CTCTTC 2 cut(s) 87, 753
EarI CTCTTC 2 cut(s) 87, 753
Ecl136II GAGCTC 1 cut(s) 276
Eco130I CCWWGG 3 cut(s) 753, 840, 1367
Eco24I GRGCYC 2 cut(s) 8, 278
Eco47I GGWCC 2 cut(s) 221, 1046
Eco53kI GAGCTC 1 cut(s) 276
Eco57I CTGAAG 1 cut(s) 482
Eco88I CYCGRG 1 cut(s) 277
EcoICRI GAGCTC 1 cut(s) 276
EcoO109I RGGNCCY 1 cut(s) 5
EcoRI GAATTC 1 cut(s) 1361
EcoRII CCWGG 3 cut(s) 258, 1339, 1399
EcoT14I CCWWGG 3 cut(s) 753, 840, 1367
EcoT22I ATGCAT 3 cut(s) 400, 1018, 1186
EcoT38I GRGCYC 2 cut(s) 8, 278
ErhI CCWWGG 3 cut(s) 753, 840, 1367
Esp3I CGTCTC 1 cut(s) 89
FaeI CATG 4 cut(s) 145, 640, 757, 797
FalI AAGNNNNNCTT 2 cut(s) 837, 869
FaqI GGGAC 2 cut(s) 319, 487
FatI CATG 4 cut(s) 141, 636, 753, 793
FauNDI CATATG 1 cut(s) 1012
FbaI TGATCA 1 cut(s) 1225
FokI GGATG 2 cut(s) 158, 341
FriOI GRGCYC 2 cut(s) 8, 278
FspBI CTAG 3 cut(s) 476, 995, 1062
GlaI GCGC 1 cut(s) 464
GsuI CTGGAG 1 cut(s) 454
HaeII RGCGCY 1 cut(s) 466
HaeIII GGCC 2 cut(s) 6, 451
HapII CCGG 3 cut(s) 89, 889, 1420
HgaI GACGC 1 cut(s) 530
HhaI GCGC 1 cut(s) 465
Hin1II CATG 4 cut(s) 145, 640, 757, 797
Hin6I GCGC 1 cut(s) 463
HinP1I GCGC 1 cut(s) 463
HincII GTYRAC 1 cut(s) 423
HindII GTYRAC 1 cut(s) 423
HinfI GANTC 3 cut(s) 190, 344, 964
HpaII CCGG 3 cut(s) 89, 889, 1420
HphI GGTGA 4 cut(s) 302, 344, 842, 928
Hpy166II GTNNAC 4 cut(s) 423, 791, 1162, 1397
Hpy188I TCNGA 6 cut(s) 240, 394, 824, 858, 963, 1033
Hpy188III TCNNGA 5 cut(s) 187, 194, 637, 974, 1082
Hpy8I GTNNAC 4 cut(s) 423, 791, 1162, 1397
HpyAV CCTTC 5 cut(s) 55, 575, 913, 1079, 1106
HpyCH4III ACNGT 3 cut(s) 298, 708, 1300
HpyCH4IV ACGT 3 cut(s) 98, 234, 692
HpyCH4V TGCA 9 cut(s) 41, 398, 484, 533, 932, 1016, 1070, 1184, 1328
HpyF10VI GCNNNNNNNGC 2 cut(s) 481, 530
HpyF3I CTNAG 5 cut(s) 950, 960, 1020, 1032, 1260
HpySE526I ACGT 3 cut(s) 98, 234, 692
Hsp92II CATG 4 cut(s) 145, 640, 757, 797
HspAI GCGC 1 cut(s) 463
Ksp22I TGATCA 1 cut(s) 1225
Kzo9I GATC 4 cut(s) 75, 183, 1123, 1225
LmnI GCTCC 1 cut(s) 16
LweI GCATC 3 cut(s) 385, 1025, 1193
MabI ACCWGGT 2 cut(s) 1339, 1399
MaeI CTAG 3 cut(s) 476, 995, 1062
MaeII ACGT 3 cut(s) 98, 234, 692
MaeIII GTNAC 4 cut(s) 116, 569, 614, 1037
MalI GATC 4 cut(s) 77, 185, 1125, 1227
MboI GATC 4 cut(s) 75, 183, 1123, 1225
MboII GAAGA 9 cut(s) 104, 658, 677, 770, 845, 857, 871, 907, 1091
MhlI GDGCHC 3 cut(s) 8, 278, 1261
MlyI GAGTC 1 cut(s) 973
MmeI TCCRAC 1 cut(s) 306
MnlI CCTC 5 cut(s) 429, 462, 560, 754, 1254
Mph1103I ATGCAT 3 cut(s) 400, 1018, 1186
MroXI GAANNNNTTC 2 cut(s) 234, 849
MseI TTAA 2 cut(s) 138, 489
MspA1I CMGCKG 1 cut(s) 56
MspI CCGG 3 cut(s) 89, 889, 1420
MspR9I CCNGG 4 cut(s) 89, 260, 1341, 1401
Mva1269I GAATGC 1 cut(s) 260
MvaI CCWGG 3 cut(s) 260, 1341, 1401
MwoI GCNNNNNNNGC 2 cut(s) 481, 530
NciI CCSGG 1 cut(s) 89
NcoI CCATGG 1 cut(s) 753
NdeI CATATG 1 cut(s) 1012
NdeII GATC 4 cut(s) 75, 183, 1123, 1225
NlaIII CATG 4 cut(s) 145, 640, 757, 797
NlaIV GGNNCC 3 cut(s) 6, 751, 1345
NmuCI GTSAC 1 cut(s) 614
NsiI ATGCAT 3 cut(s) 400, 1018, 1186
PaeR7I CTCGAG 1 cut(s) 277
PagI TCATGA 1 cut(s) 636
PcsI WCGNNNNNNNCGW 1 cut(s) 1390
PctI GAATGC 1 cut(s) 260
PdmI GAANNNNTTC 2 cut(s) 234, 849
PfeI GAWTC 2 cut(s) 190, 344
PleI GAGTC 1 cut(s) 972
PpsI GAGTC 1 cut(s) 972
Psp124BI GAGCTC 1 cut(s) 278
Psp1406I AACGTT 1 cut(s) 234
Psp6I CCWGG 3 cut(s) 258, 1339, 1399
PspGI CCWGG 3 cut(s) 258, 1339, 1399
PspN4I GGNNCC 3 cut(s) 6, 751, 1345
PspOMI GGGCCC 1 cut(s) 4
PspPI GGNCC 4 cut(s) 4, 5, 221, 1046
PspXI VCTCGAGB 1 cut(s) 277
PsrI GAACNNNNNNTAC 2 cut(s) 1387, 1419
PstI CTGCAG 1 cut(s) 486
PstNI CAGNNNCTG 1 cut(s) 302
PvuII CAGCTG 1 cut(s) 56
RsaI GTAC 4 cut(s) 790, 838, 873, 1176
RsaNI GTAC 4 cut(s) 789, 837, 872, 1175
SacI GAGCTC 1 cut(s) 278
SaqAI TTAA 2 cut(s) 138, 489
Sau3AI GATC 4 cut(s) 75, 183, 1123, 1225
Sau96I GGNCC 4 cut(s) 4, 5, 221, 1046
SchI GAGTC 1 cut(s) 973
ScrFI CCNGG 4 cut(s) 89, 260, 1341, 1401
SduI GDGCHC 3 cut(s) 8, 278, 1261
SexAI ACCWGGT 2 cut(s) 1339, 1399
SfaNI GCATC 3 cut(s) 385, 1025, 1193
SfcI CTRYAG 1 cut(s) 482
Sfr274I CTCGAG 1 cut(s) 277
SinI GGWCC 2 cut(s) 221, 1046
SlaI CTCGAG 1 cut(s) 277
SmlI CTYRAG 1 cut(s) 277
SmoI CTYRAG 1 cut(s) 277
SsiI CCGC 1 cut(s) 998
SspI AATATT 2 cut(s) 133, 205
SspMI CTAG 3 cut(s) 476, 995, 1062
SstI GAGCTC 1 cut(s) 278
StyD4I CCNGG 4 cut(s) 87, 258, 1339, 1399
StyI CCWWGG 3 cut(s) 753, 840, 1367
TaaI ACNGT 3 cut(s) 298, 708, 1300
TaiI ACGT 3 cut(s) 101, 237, 695
TaqI TCGA 2 cut(s) 186, 278
TatI WGTACW 1 cut(s) 788
TfiI GAWTC 2 cut(s) 190, 344
Tru1I TTAA 2 cut(s) 138, 489
Tru9I TTAA 2 cut(s) 138, 489
TscAI CASTG 5 cut(s) 375, 437, 711, 1048, 1147
TseFI GTSAC 1 cut(s) 614
Tsp45I GTSAC 1 cut(s) 614
TspDTI ATGAA 9 cut(s) 162, 243, 267, 351, 392, 573, 653, 735, 1002
TspGWI ACGGA 2 cut(s) 68, 354
TspRI CASTG 5 cut(s) 375, 437, 711, 1048, 1147
VpaK11BI GGWCC 2 cut(s) 221, 1046
XapI RAATTY 5 cut(s) 28, 34, 440, 560, 1361
XhoI CTCGAG 1 cut(s) 277
XmnI GAANNNNTTC 2 cut(s) 234, 849
XspI CTAG 3 cut(s) 476, 995, 1062
Zsp2I ATGCAT 3 cut(s) 400, 1018, 1186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.