Rorug01G0187300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0006015
Physical Location & Seq
Reverse (-)
25887999 .. 25889855
1857 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0187300.1

Sequence Viewer

Length: 1368 bp
ATGGGGAAACCCAACTCGACGAACACTTTTGATTTCATGCTTAGAGGTCTCAGAAGAAAGTGGCAGGTGAAAGGGGGTTGGCAACTTATTGATTTGCCTAATGATTTCTATATTGTGAAATTTAACCTTGAAGAGGACATGAACTATGCTCTGTGTGGAGGTCCATGGATTTTGGCTGGCCAAACTCTTATTGTCAGGAAATGGAGGCCAGATTTTGACCCTATGAAGGAATTCATTGGGAAGATGGCTCTCTGGGTGAGAATCTTTGGTTTACCTGTCAAATTCTTCAAGGACTTCACTGTGGCAAAAATTGGTAAAATCATGGGAGATGTGGTTAAAGTTGACAAACTCACTGTGGGTCAAGCTAGAGGACAATTTGCTAGGGTGTGCATTGAGGTTGATCTTAGCAAGCCCCTGCGTCCTTTTGTTGAGGTTGAATCCATTGCCTATCAGGTTGTCTATGAAGGGATCTCATTGATTTGTTTTGAGTGTGGATGCCTTGGGCATGCCAAAGATAAATGCCCCACTATCAAAGTTACGGATAATATTCATCCCAAACCACAAACTAGAAACTGTGATAATGAGATGACTATTGCCAATAATGATGAGAAGATGGGGTATGATGTATGTATGCAGCATGTTGCTCCACATGGTGAGGTGATTAAAGAAGATATGGGCCTATGGATGTTGATGAGCTACAGGAATAAAAAGAAAAATGGTGATGCTATCAATACCAAGAAAAACTCTGTTACTGGCTCTAGATTTACTATTCTTCAGGATGAGACTGTGGCTGACTCTGATTTTCCTGAAAAGACCATTAATGCACATAATGATGAACAGTCCCCTACCATTGTGAAACTGTGGCAGAGTTTCCAAGAGAGAAAAAATAAGATCCAAAGTCCTATAAAAAACAAGCTTGTATCTACCCCTGCTGGCACTTCCTTTAGTCGACCACCTGACACTAAGAAAACTGCTCACCTCATGGATAAGCCTACTAGCCACAAATCTAAAAACCTGCCAAGAGCCCCAATGAAAGATGTTTCCAATTTAGGAAGTAGCATTGGATCAAAGCCTGACCTACAGTATCAGAGAAAAACCAAAGGGTTGGTCAATCCTGGTTCCAAACATCAGACTCATGTTTTCAAGAAACTTTCTTTTGAAAATGTGAGTTCTAATGTTTTGGGAGATGGGATCTCAGCCATCTTTGGGCATACTCCCCCTGATGAGATTTCTGGCAATACTGGACTACAAAACTCAATTTCTCATGACACTGAGAATTTTGTTGAGAACACTTTTGCTGAGAATAGCATGGTGACCATTGAGAATAGTCTGCCCTCCCATGATGGTGAGGGTCTGGTTGATGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

455

Amino Acids

50.98

Weight (kDa)

8.7

Isoelectric Point (pI)

34.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 13 - 75 6.2e-12 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 55
Acc36I ACCTGC 2 cut(s) 55, 1023
AccI GTMKAC 1 cut(s) 949
AclWI GGATC 4 cut(s) 476, 886, 1072, 1199
AcoI YGGCCR 1 cut(s) 178
AcsI RAATTY 4 cut(s) 119, 230, 281, 1276
AcuI CTGAAG 1 cut(s) 758
AdeI CACNNNGTG 1 cut(s) 653
AfiI CCNNNNNNNGG 3 cut(s) 133, 226, 1206
AgsI TTSAA 5 cut(s) 131, 289, 437, 1144, 1160
AjnI CCWGG 1 cut(s) 1114
AluBI AGCT 3 cut(s) 365, 696, 916
AluI AGCT 3 cut(s) 365, 696, 916
Alw26I GTCTC 2 cut(s) 53, 776
AlwI GGATC 4 cut(s) 476, 886, 1072, 1199
AoxI GGCC 3 cut(s) 178, 206, 676
ApeKI GCWGC 1 cut(s) 634
ApoI RAATTY 4 cut(s) 119, 230, 281, 1276
ArsI GACNNNNNNTTYG 2 cut(s) 1092, 1124
AseI ATTAAT 1 cut(s) 819
Asp700I GAANNNNTTC 1 cut(s) 230
AspS9I GGNCC 2 cut(s) 161, 676
AsuHPI GGTGA 8 cut(s) 79, 268, 665, 670, 731, 968, 1324, 1358
AvaII GGWCC 1 cut(s) 161
BalI TGGCCA 1 cut(s) 180
BanII GRGCYC 1 cut(s) 1027
BbvI GCAGC 1 cut(s) 646
BccI CCATC 5 cut(s) 238, 607, 1181, 1208, 1337
BciT130I CCWGG 1 cut(s) 1116
BcoDI GTCTC 2 cut(s) 53, 776
BfaI CTAG 5 cut(s) 366, 381, 567, 759, 996
BfmI CTRYAG 2 cut(s) 697, 1079
BfuAI ACCTGC 2 cut(s) 55, 1023
BisI GCNGC 1 cut(s) 635
BlsI GCNGC 1 cut(s) 636
Bme1390I CCNGG 1 cut(s) 1116
Bme18I GGWCC 1 cut(s) 161
BmgT120I GGNCC 2 cut(s) 161, 676
BmiI GGNNCC 1 cut(s) 1120
BmrFI CCNGG 1 cut(s) 1116
BmsI GCATC 3 cut(s) 485, 712, 1351
BsaI GGTCTC 1 cut(s) 53
BsaJI CCNNGG 2 cut(s) 164, 499
BsaXI ACNNNNNCTCC 2 cut(s) 318, 348
Bsc4I CCNNNNNNNGG 3 cut(s) 133, 226, 1206
Bse1I ACTGG 2 cut(s) 757, 1246
Bse3DI GCAATG 1 cut(s) 441
BseBI CCWGG 1 cut(s) 1116
BseDI CCNNGG 2 cut(s) 164, 499
BseGI GGATG 4 cut(s) 500, 550, 690, 784
BseLI CCNNNNNNNGG 3 cut(s) 133, 226, 1206
BseMI GCAATG 1 cut(s) 441
BseMII CTCAG 4 cut(s) 64, 1209, 1263, 1290
BseNI ACTGG 2 cut(s) 757, 1246
BseXI GCAGC 1 cut(s) 646
BshFI GGCC 3 cut(s) 180, 208, 678
BslFI GGGAC 1 cut(s) 826
BslI CCNNNNNNNGG 3 cut(s) 133, 226, 1206
BsmAI GTCTC 2 cut(s) 53, 776
BsmFI GGGAC 1 cut(s) 826
BsnI GGCC 3 cut(s) 180, 208, 678
Bso31I GGTCTC 1 cut(s) 53
Bsp1286I GDGCHC 1 cut(s) 1027
Bsp143I GATC 5 cut(s) 400, 468, 891, 1064, 1191
Bsp19I CCATGG 1 cut(s) 164
BspANI GGCC 3 cut(s) 180, 208, 678
BspCNI CTCAG 4 cut(s) 63, 1208, 1264, 1291
BspHI TCATGA 1 cut(s) 1264
BspLI GGNNCC 1 cut(s) 1120
BspMI ACCTGC 2 cut(s) 55, 1023
BspPI GGATC 4 cut(s) 476, 886, 1072, 1199
BspTNI GGTCTC 1 cut(s) 53
BsrDI GCAATG 1 cut(s) 441
BsrI ACTGG 2 cut(s) 757, 1246
BssECI CCNNGG 2 cut(s) 164, 499
BssMI GATC 5 cut(s) 400, 468, 891, 1064, 1191
BssT1I CCWWGG 2 cut(s) 164, 499
Bst2UI CCWGG 1 cut(s) 1116
Bst4CI ACNGT 7 cut(s) 301, 355, 575, 787, 840, 861, 1083
Bst6I CTCTTC 1 cut(s) 126
BstC8I GCNNGC 4 cut(s) 178, 410, 507, 934
BstDEI CTNAG 7 cut(s) 41, 50, 404, 963, 1195, 1272, 1299
BstDSI CCRYGG 1 cut(s) 164
BstEII GGTNACC 1 cut(s) 1312
BstENI CCTNNNNNAGG 1 cut(s) 131
BstF5I GGATG 4 cut(s) 500, 550, 690, 784
BstKTI GATC 5 cut(s) 403, 471, 894, 1067, 1194
BstMAI GTCTC 2 cut(s) 53, 776
BstMBI GATC 5 cut(s) 400, 468, 891, 1064, 1191
BstNI CCWGG 1 cut(s) 1116
BstNSI RCATGY 2 cut(s) 509, 641
BstPI GGTNACC 1 cut(s) 1312
BstSCI CCNGG 1 cut(s) 1114
BstSFI CTRYAG 2 cut(s) 697, 1079
BstV1I GCAGC 1 cut(s) 646
BstX2I RGATCY 3 cut(s) 468, 891, 1191
BstXI CCANNNNNNTGG 1 cut(s) 1105
BstYI RGATCY 3 cut(s) 468, 891, 1191
BsuRI GGCC 3 cut(s) 180, 208, 678
BtgI CCRYGG 1 cut(s) 164
BtsCI GGATG 4 cut(s) 500, 550, 690, 784
BtsIMutI CAGTG 3 cut(s) 297, 351, 1269
BveI ACCTGC 2 cut(s) 55, 1023
Cac8I GCNNGC 4 cut(s) 178, 410, 507, 934
CciI TCATGA 1 cut(s) 1264
Cfr13I GGNCC 2 cut(s) 161, 676
CseI GACGC 1 cut(s) 407
DdeI CTNAG 7 cut(s) 41, 50, 404, 963, 1195, 1272, 1299
DpnI GATC 5 cut(s) 402, 470, 893, 1066, 1193
DpnII GATC 5 cut(s) 400, 468, 891, 1064, 1191
DraIII CACNNNGTG 1 cut(s) 653
EaeI YGGCCR 1 cut(s) 178
Eam1104I CTCTTC 1 cut(s) 126
EarI CTCTTC 1 cut(s) 126
Eco130I CCWWGG 2 cut(s) 164, 499
Eco24I GRGCYC 1 cut(s) 1027
Eco31I GGTCTC 1 cut(s) 53
Eco47I GGWCC 1 cut(s) 161
Eco57I CTGAAG 1 cut(s) 758
Eco91I GGTNACC 1 cut(s) 1312
EcoNI CCTNNNNNAGG 1 cut(s) 131
EcoO65I GGTNACC 1 cut(s) 1312
EcoRI GAATTC 1 cut(s) 230
EcoRII CCWGG 1 cut(s) 1114
EcoT14I CCWWGG 2 cut(s) 164, 499
EcoT38I GRGCYC 1 cut(s) 1027
ErhI CCWWGG 2 cut(s) 164, 499
FaqI GGGAC 1 cut(s) 826
FblI GTMKAC 1 cut(s) 949
Fnu4HI GCNGC 1 cut(s) 635
FokI GGATG 4 cut(s) 507, 537, 697, 791
FriOI GRGCYC 1 cut(s) 1027
Fsp4HI GCNGC 1 cut(s) 635
FspBI CTAG 5 cut(s) 366, 381, 567, 759, 996
GluI GCNGC 1 cut(s) 635
HaeIII GGCC 3 cut(s) 180, 208, 678
HgaI GACGC 1 cut(s) 407
HincII GTYRAC 2 cut(s) 343, 950
HindII GTYRAC 2 cut(s) 343, 950
HindIII AAGCTT 1 cut(s) 914
HinfI GANTC 4 cut(s) 261, 437, 794, 1132
HphI GGTGA 8 cut(s) 79, 268, 665, 670, 731, 968, 1324, 1358
Hpy166II GTNNAC 3 cut(s) 272, 343, 950
Hpy188I TCNGA 4 cut(s) 53, 799, 1089, 1131
Hpy188III TCNNGA 6 cut(s) 196, 759, 776, 806, 1144, 1265
Hpy8I GTNNAC 3 cut(s) 272, 343, 950
Hpy99I CGWCG 1 cut(s) 22
HpyAV CCTTC 2 cut(s) 220, 458
HpyCH4III ACNGT 7 cut(s) 301, 355, 575, 787, 840, 861, 1083
HpyCH4V TGCA 3 cut(s) 390, 634, 824
HpyF3I CTNAG 7 cut(s) 41, 50, 404, 963, 1195, 1272, 1299
Kzo9I GATC 5 cut(s) 400, 468, 891, 1064, 1191
LmnI GCTCC 1 cut(s) 649
Lsp1109I GCAGC 1 cut(s) 646
LweI GCATC 3 cut(s) 485, 712, 1351
MaeI CTAG 5 cut(s) 366, 381, 567, 759, 996
MaeIII GTNAC 3 cut(s) 535, 748, 1312
MalI GATC 5 cut(s) 402, 470, 893, 1066, 1193
MboI GATC 5 cut(s) 400, 468, 891, 1064, 1191
MboII GAAGA 7 cut(s) 66, 143, 253, 277, 622, 680, 764
MflI RGATCY 3 cut(s) 468, 891, 1191
MhlI GDGCHC 1 cut(s) 1027
MlsI TGGCCA 1 cut(s) 180
MluCI AATT 8 cut(s) 119, 230, 281, 309, 374, 1045, 1257, 1276
MluNI TGGCCA 1 cut(s) 180
MlyI GAGTC 2 cut(s) 788, 1126
Mox20I TGGCCA 1 cut(s) 180
MroXI GAANNNNTTC 1 cut(s) 230
MscI TGGCCA 1 cut(s) 180
MseI TTAA 4 cut(s) 123, 336, 663, 819
MslI CAYNNNNRTG 2 cut(s) 831, 1344
Msp20I TGGCCA 1 cut(s) 180
MspR9I CCNGG 1 cut(s) 1116
MvaI CCWGG 1 cut(s) 1116
NcoI CCATGG 1 cut(s) 164
NdeII GATC 5 cut(s) 400, 468, 891, 1064, 1191
NlaIV GGNNCC 1 cut(s) 1120
NmuCI GTSAC 1 cut(s) 1312
NspI RCATGY 2 cut(s) 509, 641
PaeI GCATGC 1 cut(s) 509
PagI TCATGA 1 cut(s) 1264
PaqCI CACCTGC 1 cut(s) 55
PdmI GAANNNNTTC 1 cut(s) 230
PfeI GAWTC 2 cut(s) 261, 437
PkrI GCNGC 1 cut(s) 636
PleI GAGTC 2 cut(s) 788, 1126
PpsI GAGTC 2 cut(s) 788, 1126
PshBI ATTAAT 1 cut(s) 819
Psp6I CCWGG 1 cut(s) 1114
PspEI GGTNACC 1 cut(s) 1312
PspGI CCWGG 1 cut(s) 1114
PspN4I GGNNCC 1 cut(s) 1120
PspPI GGNCC 2 cut(s) 161, 676
PsuI RGATCY 3 cut(s) 468, 891, 1191
RseI CAYNNNNRTG 2 cut(s) 831, 1344
SalI GTCGAC 1 cut(s) 948
SaqAI TTAA 4 cut(s) 123, 336, 663, 819
SatI GCNGC 1 cut(s) 635
Sau3AI GATC 5 cut(s) 400, 468, 891, 1064, 1191
Sau96I GGNCC 2 cut(s) 161, 676
SchI GAGTC 2 cut(s) 788, 1126
ScrFI CCNGG 1 cut(s) 1116
SduI GDGCHC 1 cut(s) 1027
SfaNI GCATC 3 cut(s) 485, 712, 1351
SfcI CTRYAG 2 cut(s) 697, 1079
SinI GGWCC 1 cut(s) 161
SmiMI CAYNNNNRTG 2 cut(s) 831, 1344
SphI GCATGC 1 cut(s) 509
Sse9I AATT 8 cut(s) 119, 230, 281, 309, 374, 1045, 1257, 1276
SspI AATATT 1 cut(s) 547
SspMI CTAG 5 cut(s) 366, 381, 567, 759, 996
StyD4I CCNGG 1 cut(s) 1114
StyI CCWWGG 2 cut(s) 164, 499
TaaI ACNGT 7 cut(s) 301, 355, 575, 787, 840, 861, 1083
TaqI TCGA 2 cut(s) 17, 949
TasI AATT 8 cut(s) 119, 230, 281, 309, 374, 1045, 1257, 1276
TfiI GAWTC 2 cut(s) 261, 437
Tru1I TTAA 4 cut(s) 123, 336, 663, 819
Tru9I TTAA 4 cut(s) 123, 336, 663, 819
TscAI CASTG 3 cut(s) 304, 358, 1276
TseFI GTSAC 1 cut(s) 1312
TseI GCWGC 1 cut(s) 634
Tsp45I GTSAC 1 cut(s) 1312
TspDTI ATGAA 8 cut(s) 25, 155, 223, 239, 477, 539, 849, 1046
TspGWI ACGGA 1 cut(s) 554
TspRI CASTG 3 cut(s) 304, 358, 1276
VpaK11BI GGWCC 1 cut(s) 161
VspI ATTAAT 1 cut(s) 819
XagI CCTNNNNNAGG 1 cut(s) 131
XapI RAATTY 4 cut(s) 119, 230, 281, 1276
XbaI TCTAGA 1 cut(s) 758
XceI RCATGY 2 cut(s) 509, 641
XmiI GTMKAC 1 cut(s) 949
XmnI GAANNNNTTC 1 cut(s) 230
XspI CTAG 5 cut(s) 366, 381, 567, 759, 996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.