FvH4_7g11340

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0007373
Physical Location & Seq
Forward (+)
10558946 .. 10559680
735 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g11340.t1

Sequence Viewer

Length: 735 bp
ATGACGCTGAAACAATCACTTGTAATGTTGTCATTTTTTGCAGGTGTCTCAGGGGATGGTTTTTGGGCGAGGGACATAACGTTTGAGAATACAGCCGGTCCACAAAAGCACCAAGCAGTGGCATTGAGGGTTAGCTCCGACCTGGCAGTGTTTTACCGATGTAGCATCAAGGGATACCAAGACAGTCTCTACGCCCACTCCCTAAGGCAATTCTACCGTGACAGTCACATATACGGCACCATCGATTTCATATTTGGTGACGCCTCTGTCGTGCTACAAAATTGCGACATTTTAGTGAGGAGGCCATTGGGGCACCAATCCAACATGATCACTGCTCAAGGACGTGACAACCCGGCAGAGAACACGGGCAATGTTATTCACGGTTGTCGCGTTAGGCCTGCGAGGGAGTTTGAGGGGGTTAAGGGTTCGTTTAAGACTTACCTTGATCGGCCATGGCATAAATACTCGAGGACAGTGTTCTTAAAGACTGATTTGGATGGTTTGGTTGATCCAAAGGGGTGGACGGAATGGAGAGGTAGTTTTGCAATTTCAACACTGTATTATGGGGAGTATATGAATGTAGGGCAGGGTGCCTCAACTGATAAGAGGGTTAGATGGCCTAGTTTTCATGTGCTTAATAGTCCTCGGGAGGTCAGCCCTTACTCAATTAGCCATTTCATTCAAGGAGAGTCATGGATTCTAGGGACCGGTGTGCCGGTTTGGCTTGGAATATAA

Protein Analysis

245

Amino Acids

27.58

Weight (kDa)

8.94

Isoelectric Point (pI)

39.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 15 - 230 5.8e-100 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 32
AasI GACNNNNNNGTC 1 cut(s) 266
Acc36I ACCTGC 1 cut(s) 32
AccB1I GGYRCC 3 cut(s) 236, 312, 590
AccB7I CCANNNNNTGG 1 cut(s) 118
AccII CGCG 1 cut(s) 390
AclI AACGTT 1 cut(s) 80
AclWI GGATC 1 cut(s) 503
AcoI YGGCCR 1 cut(s) 449
AcyI GRCGYC 1 cut(s) 261
AfiI CCNNNNNNNGG 1 cut(s) 118
AgeI ACCGGT 1 cut(s) 707
AgsI TTSAA 2 cut(s) 552, 683
AjiI CACGTC 1 cut(s) 344
AjnI CCWGG 1 cut(s) 141
AluBI AGCT 1 cut(s) 135
AluI AGCT 1 cut(s) 135
Alw26I GTCTC 2 cut(s) 52, 191
AlwI GGATC 1 cut(s) 503
Ama87I CYCGRG 2 cut(s) 466, 645
AoxI GGCC 4 cut(s) 302, 395, 449, 617
ArsI GACNNNNNNTTYG 2 cut(s) 65, 97
AsiGI ACCGGT 1 cut(s) 707
AspS9I GGNCC 2 cut(s) 98, 705
AsuC2I CCSGG 1 cut(s) 353
AsuHPI GGTGA 1 cut(s) 269
AvaI CYCGRG 2 cut(s) 466, 645
AvaII GGWCC 2 cut(s) 98, 705
AxyI CCTNAGG 1 cut(s) 203
BaeGI GKGCMC 1 cut(s) 315
BanI GGYRCC 3 cut(s) 236, 312, 590
BccI CCATC 4 cut(s) 50, 248, 491, 609
BceAI ACGGC 1 cut(s) 250
BciT130I CCWGG 1 cut(s) 143
BciVI GTATCC 1 cut(s) 167
BclI TGATCA 1 cut(s) 327
BcnI CCSGG 1 cut(s) 353
BcoDI GTCTC 2 cut(s) 52, 191
BfaI CTAG 2 cut(s) 621, 701
BfuAI ACCTGC 1 cut(s) 32
BfuI GTATCC 1 cut(s) 167
BglI GCCNNNNNGGC 2 cut(s) 310, 721
Bme1390I CCNGG 2 cut(s) 143, 353
Bme18I GGWCC 2 cut(s) 98, 705
BmeT110I CYCGRG 2 cut(s) 466, 645
BmgBI CACGTC 1 cut(s) 344
BmgT120I GGNCC 2 cut(s) 98, 705
BmiI GGNNCC 4 cut(s) 238, 314, 592, 706
BmrFI CCNGG 2 cut(s) 143, 353
BmsI GCATC 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 321
BpuMI CCSGG 1 cut(s) 353
Bsa29I ATCGAT 1 cut(s) 243
BsaHI GRCGYC 1 cut(s) 261
BsaJI CCNNGG 2 cut(s) 452, 644
BsaWI WCCGGW 1 cut(s) 707
BsaXI ACNNNNNCTCC 4 cut(s) 182, 212, 523, 553
Bsc4I CCNNNNNNNGG 1 cut(s) 118
Bse118I RCCGGY 3 cut(s) 95, 707, 715
Bse21I CCTNAGG 1 cut(s) 203
Bse3DI GCAATG 1 cut(s) 376
BseBI CCWGG 1 cut(s) 143
BseCI ATCGAT 1 cut(s) 243
BseDI CCNNGG 2 cut(s) 452, 644
BseGI GGATG 2 cut(s) 61, 502
BseLI CCNNNNNNNGG 1 cut(s) 118
BseMI GCAATG 1 cut(s) 376
BseMII CTCAG 1 cut(s) 63
BseRI GAGGAG 1 cut(s) 313
BseSI GKGCMC 1 cut(s) 315
Bsh1236I CGCG 1 cut(s) 390
BshFI GGCC 4 cut(s) 304, 397, 451, 619
BshNI GGYRCC 3 cut(s) 236, 312, 590
BshTI ACCGGT 1 cut(s) 707
BshVI ATCGAT 1 cut(s) 243
BsiHKCI CYCGRG 2 cut(s) 466, 645
BsiSI CCGG 4 cut(s) 96, 353, 708, 716
BslFI GGGAC 2 cut(s) 86, 718
BslI CCNNNNNNNGG 1 cut(s) 118
BsmAI GTCTC 2 cut(s) 52, 191
BsmFI GGGAC 2 cut(s) 86, 718
BsnI GGCC 4 cut(s) 304, 397, 451, 619
BsoBI CYCGRG 2 cut(s) 466, 645
Bsp1286I GDGCHC 1 cut(s) 315
Bsp143I GATC 3 cut(s) 327, 445, 508
Bsp19I CCATGG 1 cut(s) 452
BspANI GGCC 4 cut(s) 304, 397, 451, 619
BspCNI CTCAG 1 cut(s) 62
BspDI ATCGAT 1 cut(s) 243
BspFNI CGCG 1 cut(s) 390
BspLI GGNNCC 4 cut(s) 238, 314, 592, 706
BspMI ACCTGC 1 cut(s) 32
BspPI GGATC 1 cut(s) 503
BspT107I GGYRCC 3 cut(s) 236, 312, 590
BsrDI GCAATG 1 cut(s) 376
BsrFI RCCGGY 3 cut(s) 95, 707, 715
BssAI RCCGGY 3 cut(s) 95, 707, 715
BssECI CCNNGG 2 cut(s) 452, 644
BssMI GATC 3 cut(s) 327, 445, 508
BssNI GRCGYC 1 cut(s) 261
BssT1I CCWWGG 1 cut(s) 452
Bst2UI CCWGG 1 cut(s) 143
Bst4CI ACNGT 6 cut(s) 185, 218, 224, 383, 475, 558
BstACI GRCGYC 1 cut(s) 261
BstC8I GCNNGC 1 cut(s) 399
BstDEI CTNAG 2 cut(s) 49, 203
BstDSI CCRYGG 1 cut(s) 452
BstF5I GGATG 2 cut(s) 61, 502
BstFNI CGCG 1 cut(s) 390
BstKTI GATC 3 cut(s) 330, 448, 511
BstMAI GTCTC 2 cut(s) 52, 191
BstMBI GATC 3 cut(s) 327, 445, 508
BstMWI GCNNNNNNNGC 2 cut(s) 310, 721
BstNI CCWGG 1 cut(s) 143
BstSCI CCNGG 2 cut(s) 141, 351
BstSLI GKGCMC 1 cut(s) 315
BstUI CGCG 1 cut(s) 390
BstXI CCANNNNNNTGG 1 cut(s) 519
Bsu15I ATCGAT 1 cut(s) 243
Bsu36I CCTNAGG 1 cut(s) 203
BsuI GTATCC 1 cut(s) 167
BsuRI GGCC 4 cut(s) 304, 397, 451, 619
BsuTUI ATCGAT 1 cut(s) 243
BtgI CCRYGG 1 cut(s) 452
BtrI CACGTC 1 cut(s) 344
BtsCI GGATG 2 cut(s) 61, 502
BtsI GCAGTG 3 cut(s) 123, 153, 330
BtsIMutI CAGTG 5 cut(s) 123, 153, 330, 480, 554
BveI ACCTGC 1 cut(s) 32
Cac8I GCNNGC 1 cut(s) 399
Cfr10I RCCGGY 3 cut(s) 95, 707, 715
Cfr13I GGNCC 2 cut(s) 98, 705
ClaI ATCGAT 1 cut(s) 243
CseI GACGC 2 cut(s) 13, 269
CspAI ACCGGT 1 cut(s) 707
CviAII CATG 4 cut(s) 325, 453, 629, 693
CviJI RGCY 9 cut(s) 95, 135, 304, 397, 451, 619, 657, 672, 724
CviKI_1 RGCY 9 cut(s) 95, 135, 304, 397, 451, 619, 657, 672, 724
DdeI CTNAG 2 cut(s) 49, 203
DpnI GATC 3 cut(s) 329, 447, 510
DpnII GATC 3 cut(s) 327, 445, 508
DrdI GACNNNNNNGTC 1 cut(s) 266
DseDI GACNNNNNNGTC 1 cut(s) 266
EaeI YGGCCR 1 cut(s) 449
Eco130I CCWWGG 1 cut(s) 452
Eco147I AGGCCT 1 cut(s) 397
Eco47I GGWCC 2 cut(s) 98, 705
Eco81I CCTNAGG 1 cut(s) 203
Eco88I CYCGRG 2 cut(s) 466, 645
EcoRII CCWGG 1 cut(s) 141
EcoT14I CCWWGG 1 cut(s) 452
ErhI CCWWGG 1 cut(s) 452
FaeI CATG 4 cut(s) 328, 456, 632, 696
FaqI GGGAC 2 cut(s) 86, 718
FatI CATG 4 cut(s) 324, 452, 628, 692
FbaI TGATCA 1 cut(s) 327
FokI GGATG 2 cut(s) 68, 509
FspBI CTAG 2 cut(s) 621, 701
HaeIII GGCC 4 cut(s) 304, 397, 451, 619
HapII CCGG 4 cut(s) 96, 353, 708, 716
HgaI GACGC 2 cut(s) 13, 269
Hin1I GRCGYC 1 cut(s) 261
Hin1II CATG 4 cut(s) 328, 456, 632, 696
HinfI GANTC 2 cut(s) 689, 697
HpaII CCGG 4 cut(s) 96, 353, 708, 716
HphI GGTGA 1 cut(s) 269
Hpy166II GTNNAC 2 cut(s) 101, 522
Hpy188I TCNGA 1 cut(s) 139
Hpy188III TCNNGA 1 cut(s) 647
Hpy8I GTNNAC 2 cut(s) 101, 522
HpyCH4III ACNGT 6 cut(s) 185, 218, 224, 383, 475, 558
HpyCH4IV ACGT 2 cut(s) 80, 343
HpyCH4V TGCA 2 cut(s) 41, 545
HpyF10VI GCNNNNNNNGC 2 cut(s) 310, 721
HpyF3I CTNAG 2 cut(s) 49, 203
HpySE526I ACGT 2 cut(s) 80, 343
Hsp92I GRCGYC 1 cut(s) 261
Hsp92II CATG 4 cut(s) 328, 456, 632, 696
Ksp22I TGATCA 1 cut(s) 327
Kzo9I GATC 3 cut(s) 327, 445, 508
LmnI GCTCC 1 cut(s) 140
LweI GCATC 1 cut(s) 174
MaeI CTAG 2 cut(s) 621, 701
MaeII ACGT 2 cut(s) 80, 343
MaeIII GTNAC 4 cut(s) 218, 224, 257, 344
MalI GATC 3 cut(s) 329, 447, 510
MboI GATC 3 cut(s) 327, 445, 508
MhlI GDGCHC 1 cut(s) 315
MluCI AATT 4 cut(s) 209, 280, 546, 666
MlyI GAGTC 1 cut(s) 698
MmeI TCCRAC 2 cut(s) 162, 345
MseI TTAA 4 cut(s) 420, 432, 482, 636
MslI CAYNNNNRTG 1 cut(s) 293
MspI CCGG 4 cut(s) 96, 353, 708, 716
MspR9I CCNGG 2 cut(s) 143, 353
MvaI CCWGG 1 cut(s) 143
MvnI CGCG 1 cut(s) 390
MwoI GCNNNNNNNGC 2 cut(s) 310, 721
NciI CCSGG 1 cut(s) 353
NcoI CCATGG 1 cut(s) 452
NdeII GATC 3 cut(s) 327, 445, 508
NlaIII CATG 4 cut(s) 328, 456, 632, 696
NlaIV GGNNCC 4 cut(s) 238, 314, 592, 706
NmuCI GTSAC 4 cut(s) 218, 224, 257, 344
PaeR7I CTCGAG 1 cut(s) 466
PaqCI CACCTGC 1 cut(s) 32
PceI AGGCCT 1 cut(s) 397
PcsI WCGNNNNNNNCGW 3 cut(s) 240, 267, 387
PfeI GAWTC 1 cut(s) 697
PflMI CCANNNNNTGG 1 cut(s) 118
PinAI ACCGGT 1 cut(s) 707
PleI GAGTC 1 cut(s) 697
PpsI GAGTC 1 cut(s) 697
Psp1406I AACGTT 1 cut(s) 80
Psp6I CCWGG 1 cut(s) 141
PspGI CCWGG 1 cut(s) 141
PspN4I GGNNCC 4 cut(s) 238, 314, 592, 706
PspPI GGNCC 2 cut(s) 98, 705
PspXI VCTCGAGB 1 cut(s) 466
RseI CAYNNNNRTG 1 cut(s) 293
SaqAI TTAA 4 cut(s) 420, 432, 482, 636
Sau3AI GATC 3 cut(s) 327, 445, 508
Sau96I GGNCC 2 cut(s) 98, 705
SchI GAGTC 1 cut(s) 698
ScrFI CCNGG 2 cut(s) 143, 353
SduI GDGCHC 1 cut(s) 315
SetI ASST 8 cut(s) 46, 83, 137, 144, 346, 444, 538, 654
SfaNI GCATC 1 cut(s) 174
Sfr274I CTCGAG 1 cut(s) 466
SinI GGWCC 2 cut(s) 98, 705
SlaI CTCGAG 1 cut(s) 466
SmiMI CAYNNNNRTG 1 cut(s) 293
SmlI CTYRAG 2 cut(s) 336, 466
SmoI CTYRAG 2 cut(s) 336, 466
Sse9I AATT 4 cut(s) 209, 280, 546, 666
SseBI AGGCCT 1 cut(s) 397
SspMI CTAG 2 cut(s) 621, 701
StuI AGGCCT 1 cut(s) 397
StyD4I CCNGG 2 cut(s) 141, 351
StyI CCWWGG 1 cut(s) 452
TaaI ACNGT 6 cut(s) 185, 218, 224, 383, 475, 558
TaiI ACGT 2 cut(s) 83, 346
TaqI TCGA 2 cut(s) 243, 467
TasI AATT 4 cut(s) 209, 280, 546, 666
TfiI GAWTC 1 cut(s) 697
Tru1I TTAA 4 cut(s) 420, 432, 482, 636
Tru9I TTAA 4 cut(s) 420, 432, 482, 636
TscAI CASTG 5 cut(s) 123, 153, 337, 480, 561
TseFI GTSAC 4 cut(s) 218, 224, 257, 344
Tsp45I GTSAC 4 cut(s) 218, 224, 257, 344
TspDTI ATGAA 4 cut(s) 238, 590, 617, 667
TspGWI ACGGA 1 cut(s) 539
TspRI CASTG 5 cut(s) 123, 153, 337, 480, 561
Van91I CCANNNNNTGG 1 cut(s) 118
VpaK11BI GGWCC 2 cut(s) 98, 705
XhoI CTCGAG 1 cut(s) 466
XspI CTAG 2 cut(s) 621, 701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.