MD07G1118200.v1.1

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007373
Physical Location & Seq
Forward (+)
14595493 .. 14596861
1369 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1118200.v1.1.491

Sequence Viewer

Length: 1074 bp
ATGCAAGACGGAGGGCTTTTGGCATCATGGAATCCAAGAACATCCAGGGCTGATTTTCTGGTGGCAAAAGATGGCTCTGGCACAAATAGAACAGTGAATGAGGCACTGATTGCACTTGCTAGAATGGGTCACCGGCGAACCCAAAGAATCATAATCTATGTGAAATCCGGCATGTACGCAGAGAAGGTGGAGATTCATAGAGATGTAAAGAATGTGATGCTTGTTGGTGATGGCATTGACAAAACTATAATCACCGGTAGCCACAGTGTCGTCGGAGATGACTCCACTCGGAGCTCAGCTACGGTTGGTAAGTCTAGAGGTCTAATCACTTGGCCAAGATTTATAGTGATCAAAGTCTTGGTCCTCAAAAATCACCAGGCAGTGGCACTGAGGGTGAGCTCAGACCTCGCGGTGTTTTACCGGTGCAGCTTCAAAGCGTACCAAGACACTGTCTATGCCCACTCCAATCGACAATTCTACCGCGACTGTCAAGTATATGGCACCATCGATTTCATATTTGGTGACGCCTATATGGTGATGCAAAATTGTGACATTTTCGTGAGGAGGCCAATGAAGTCCCAAGCTAACATGATCACCGCGCAAGGTCGCGACAACCCGGAAGAGAACTCAGGCATTGCAATCCACGGTTGTCGCGTTAGACCTGCACCGGAATTCGCAGGCGTTAAGGGTTCGTTTAGGAACTATTTGGGCAGGCCATGGAGAAAGTATTCAAGGACTGTGTTCTTGAAGACAGACTTGGATGGTTTGATTGATCCAAAGAGGTGGACAGAATGGAGGGGCAGTTATGGACTTTCAACGTTGTTTTATGGGGAGTATATGAATACAGGGCCAGGTGCAGCAACAGAGAAGAGGGTTAAGTGGCCTGGTTTTCATTTGGTCCTCAGCAAGTTAGTCCTTTCACAGTTGGTAATTTCATTCAAGGACAGAATTGGATTCCAGTCACTGGTGGTGTGCCATTCTGGCCTGGAATATAATAAAGATATTATACTATGGTCGGAGGCTTGGAGCCCCTCTCTATGTAATCAGAAGGGTTCTTTAGAGAAAGGCCCTTGA

Protein Analysis

358

Amino Acids

40.21

Weight (kDa)

9.66

Isoelectric Point (pI)

30.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 19 - 303 2.3e-113 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 670
AccB1I GGYRCC 1 cut(s) 500
AccB7I CCANNNNNTGG 2 cut(s) 382, 964
AccII CGCG 5 cut(s) 410, 483, 599, 609, 654
AciI CCGC 3 cut(s) 410, 481, 597
AclI AACGTT 1 cut(s) 818
AclWI GGATC 1 cut(s) 767
AcoI YGGCCR 1 cut(s) 332
AcsI RAATTY 1 cut(s) 671
AcyI GRCGYC 1 cut(s) 525
AfaI GTAC 2 cut(s) 176, 440
AfiI CCNNNNNNNGG 2 cut(s) 382, 964
AgeI ACCGGT 2 cut(s) 254, 420
AgsI TTSAA 5 cut(s) 433, 732, 748, 816, 940
AjnI CCWGG 5 cut(s) 44, 375, 850, 883, 984
AjuI GAANNNNNNNTTGG 4 cut(s) 458, 490, 740, 772
AluBI AGCT 5 cut(s) 294, 299, 399, 429, 584
AluI AGCT 5 cut(s) 294, 299, 399, 429, 584
Alw21I GWGCWC 2 cut(s) 296, 401
AlwI GGATC 1 cut(s) 767
AlwNI CAGNNNCTG 1 cut(s) 964
AoxI GGCC 7 cut(s) 332, 566, 713, 848, 881, 982, 1066
ApeKI GCWGC 2 cut(s) 426, 857
ApoI RAATTY 1 cut(s) 671
ArsI GACNNNNNNTTYG 2 cut(s) 345, 377
AsiGI ACCGGT 2 cut(s) 254, 420
Asp700I GAANNNNTTC 1 cut(s) 727
AspLEI GCGC 1 cut(s) 601
AspS9I GGNCC 4 cut(s) 361, 848, 898, 1067
AsuC2I CCSGG 1 cut(s) 617
AsuHPI GGTGA 8 cut(s) 122, 239, 244, 365, 406, 533, 547, 586
AvaII GGWCC 2 cut(s) 361, 898
BalI TGGCCA 1 cut(s) 334
BanI GGYRCC 1 cut(s) 500
BanII GRGCYC 3 cut(s) 296, 401, 1031
BbsI GAAGAC 1 cut(s) 755
Bbv12I GWGCWC 2 cut(s) 296, 401
BbvCI CCTCAGC 1 cut(s) 902
BbvI GCAGC 2 cut(s) 438, 869
BccI CCATC 4 cut(s) 65, 224, 512, 755
BciT130I CCWGG 5 cut(s) 46, 377, 852, 885, 986
BclI TGATCA 2 cut(s) 348, 591
BcnI CCSGG 1 cut(s) 617
BfaI CTAG 2 cut(s) 120, 315
BfuAI ACCTGC 1 cut(s) 670
BglI GCCNNNNNGGC 1 cut(s) 981
BisI GCNGC 2 cut(s) 427, 858
BlpI GCTNAGC 1 cut(s) 295
BlsI GCNGC 2 cut(s) 428, 859
Bme1390I CCNGG 6 cut(s) 46, 377, 617, 852, 885, 986
Bme18I GGWCC 2 cut(s) 361, 898
BmgT120I GGNCC 4 cut(s) 361, 848, 898, 1067
BmiI GGNNCC 2 cut(s) 502, 1028
BmrFI CCNGG 6 cut(s) 46, 377, 617, 852, 885, 986
BmsI GCATC 3 cut(s) 32, 207, 528
BpiI GAAGAC 1 cut(s) 755
BplI GAGNNNNNCTC 2 cut(s) 1018, 1050
Bpu10I CCTNAGC 1 cut(s) 902
Bpu1102I GCTNAGC 1 cut(s) 295
BpuMI CCSGG 1 cut(s) 617
Bsa29I ATCGAT 1 cut(s) 507
BsaHI GRCGYC 1 cut(s) 525
BsaJI CCNNGG 3 cut(s) 45, 643, 716
BsaWI WCCGGW 3 cut(s) 254, 420, 667
BsaXI ACNNNNNCTCC 2 cut(s) 787, 817
Bsc4I CCNNNNNNNGG 2 cut(s) 382, 964
Bse118I RCCGGY 3 cut(s) 132, 254, 420
Bse1I ACTGG 2 cut(s) 958, 969
Bse3DI GCAATG 1 cut(s) 633
BseBI CCWGG 5 cut(s) 46, 377, 852, 885, 986
BseCI ATCGAT 1 cut(s) 507
BseDI CCNNGG 3 cut(s) 45, 643, 716
BseGI GGATG 2 cut(s) 41, 766
BseLI CCNNNNNNNGG 2 cut(s) 382, 964
BseMI GCAATG 1 cut(s) 633
BseMII CTCAG 5 cut(s) 309, 380, 414, 642, 916
BseNI ACTGG 2 cut(s) 958, 969
BseRI GAGGAG 1 cut(s) 577
BseXI GCAGC 2 cut(s) 438, 869
BsgI GTGCAG 3 cut(s) 445, 648, 876
Bsh1236I CGCG 5 cut(s) 410, 483, 599, 609, 654
BshFI GGCC 7 cut(s) 334, 568, 715, 850, 883, 984, 1068
BshNI GGYRCC 1 cut(s) 500
BshTI ACCGGT 2 cut(s) 254, 420
BshVI ATCGAT 1 cut(s) 507
BsiHKAI GWGCWC 2 cut(s) 296, 401
BsiSI CCGG 6 cut(s) 133, 168, 255, 421, 617, 668
BslFI GGGAC 1 cut(s) 562
BslI CCNNNNNNNGG 2 cut(s) 382, 964
BsmFI GGGAC 1 cut(s) 562
BsnI GGCC 7 cut(s) 334, 568, 715, 850, 883, 984, 1068
Bsp1286I GDGCHC 3 cut(s) 296, 401, 1031
Bsp143I GATC 3 cut(s) 348, 591, 772
Bsp1720I GCTNAGC 1 cut(s) 295
Bsp19I CCATGG 1 cut(s) 716
Bsp68I TCGCGA 1 cut(s) 609
BspACI CCGC 3 cut(s) 410, 481, 597
BspANI GGCC 7 cut(s) 334, 568, 715, 850, 883, 984, 1068
BspCNI CTCAG 5 cut(s) 308, 381, 413, 641, 915
BspDI ATCGAT 1 cut(s) 507
BspFNI CGCG 5 cut(s) 410, 483, 599, 609, 654
BspLI GGNNCC 2 cut(s) 502, 1028
BspMI ACCTGC 1 cut(s) 670
BspPI GGATC 1 cut(s) 767
BspT107I GGYRCC 1 cut(s) 500
BsrDI GCAATG 1 cut(s) 633
BsrFI RCCGGY 3 cut(s) 132, 254, 420
BsrI ACTGG 2 cut(s) 958, 969
BssAI RCCGGY 3 cut(s) 132, 254, 420
BssECI CCNNGG 3 cut(s) 45, 643, 716
BssMI GATC 3 cut(s) 348, 591, 772
BssNI GRCGYC 1 cut(s) 525
BssT1I CCWWGG 1 cut(s) 716
Bst2UI CCWGG 5 cut(s) 46, 377, 852, 885, 986
Bst4CI ACNGT 8 cut(s) 94, 266, 304, 451, 488, 647, 739, 924
Bst6I CTCTTC 2 cut(s) 615, 863
BstACI GRCGYC 1 cut(s) 525
BstAPI GCANNNNNTGC 1 cut(s) 110
BstC8I GCNNGC 2 cut(s) 679, 713
BstDEI CTNAG 5 cut(s) 295, 389, 400, 628, 902
BstDSI CCRYGG 2 cut(s) 643, 716
BstEII GGTNACC 1 cut(s) 128
BstF5I GGATG 2 cut(s) 41, 766
BstFNI CGCG 5 cut(s) 410, 483, 599, 609, 654
BstHHI GCGC 1 cut(s) 601
BstKTI GATC 3 cut(s) 351, 594, 775
BstMBI GATC 3 cut(s) 348, 591, 772
BstMWI GCNNNNNNNGC 2 cut(s) 110, 981
BstNI CCWGG 5 cut(s) 46, 377, 852, 885, 986
BstNSI RCATGY 1 cut(s) 175
BstPI GGTNACC 1 cut(s) 128
BstSCI CCNGG 6 cut(s) 44, 375, 615, 850, 883, 984
BstUI CGCG 5 cut(s) 410, 483, 599, 609, 654
BstV1I GCAGC 2 cut(s) 438, 869
BstV2I GAAGAC 1 cut(s) 755
BstXI CCANNNNNNTGG 1 cut(s) 783
Bsu15I ATCGAT 1 cut(s) 507
BsuRI GGCC 7 cut(s) 334, 568, 715, 850, 883, 984, 1068
BsuTUI ATCGAT 1 cut(s) 507
BtgI CCRYGG 2 cut(s) 643, 716
BtsCI GGATG 2 cut(s) 41, 766
BtsI GCAGTG 1 cut(s) 387
BtsIMutI CAGTG 7 cut(s) 99, 104, 271, 386, 387, 447, 962
BtuMI TCGCGA 1 cut(s) 609
BveI ACCTGC 1 cut(s) 670
Cac8I GCNNGC 2 cut(s) 679, 713
CaiI CAGNNNCTG 1 cut(s) 964
CfoI GCGC 1 cut(s) 601
Cfr10I RCCGGY 3 cut(s) 132, 254, 420
Cfr13I GGNCC 4 cut(s) 361, 848, 898, 1067
ClaI ATCGAT 1 cut(s) 507
CseI GACGC 1 cut(s) 533
Csp6I GTAC 2 cut(s) 175, 439
CspAI ACCGGT 2 cut(s) 254, 420
CviAII CATG 4 cut(s) 27, 172, 589, 717
CviQI GTAC 2 cut(s) 175, 439
DdeI CTNAG 5 cut(s) 295, 389, 400, 628, 902
DpnI GATC 3 cut(s) 350, 593, 774
DpnII GATC 3 cut(s) 348, 591, 772
EaeI YGGCCR 1 cut(s) 332
Eam1104I CTCTTC 2 cut(s) 615, 863
EarI CTCTTC 2 cut(s) 615, 863
Ecl136II GAGCTC 2 cut(s) 294, 399
Eco130I CCWWGG 1 cut(s) 716
Eco24I GRGCYC 3 cut(s) 296, 401, 1031
Eco47I GGWCC 2 cut(s) 361, 898
Eco53kI GAGCTC 2 cut(s) 294, 399
Eco91I GGTNACC 1 cut(s) 128
EcoICRI GAGCTC 2 cut(s) 294, 399
EcoO109I RGGNCCY 1 cut(s) 1067
EcoO65I GGTNACC 1 cut(s) 128
EcoRI GAATTC 1 cut(s) 671
EcoRII CCWGG 5 cut(s) 44, 375, 850, 883, 984
EcoT14I CCWWGG 1 cut(s) 716
EcoT38I GRGCYC 3 cut(s) 296, 401, 1031
ErhI CCWWGG 1 cut(s) 716
FaeI CATG 4 cut(s) 30, 175, 592, 720
FalI AAGNNNNNCTT 2 cut(s) 740, 772
FaqI GGGAC 1 cut(s) 562
FatI CATG 4 cut(s) 26, 171, 588, 716
FbaI TGATCA 2 cut(s) 348, 591
Fnu4HI GCNGC 2 cut(s) 427, 858
FokI GGATG 2 cut(s) 28, 773
FriOI GRGCYC 3 cut(s) 296, 401, 1031
Fsp4HI GCNGC 2 cut(s) 427, 858
FspBI CTAG 2 cut(s) 120, 315
GlaI GCGC 1 cut(s) 600
GluI GCNGC 2 cut(s) 427, 858
HaeIII GGCC 7 cut(s) 334, 568, 715, 850, 883, 984, 1068
HapII CCGG 6 cut(s) 133, 168, 255, 421, 617, 668
HgaI GACGC 1 cut(s) 533
HhaI GCGC 1 cut(s) 601
Hin1I GRCGYC 1 cut(s) 525
Hin1II CATG 4 cut(s) 30, 175, 592, 720
Hin6I GCGC 1 cut(s) 599
HinP1I GCGC 1 cut(s) 599
HinfI GANTC 5 cut(s) 31, 147, 193, 281, 954
HpaII CCGG 6 cut(s) 133, 168, 255, 421, 617, 668
HphI GGTGA 8 cut(s) 122, 239, 244, 365, 406, 533, 547, 586
Hpy166II GTNNAC 1 cut(s) 786
Hpy188I TCNGA 5 cut(s) 275, 291, 403, 1018, 1047
Hpy188III TCNNGA 4 cut(s) 315, 559, 608, 745
Hpy8I GTNNAC 1 cut(s) 786
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 2 cut(s) 178, 1042
HpyCH4III ACNGT 8 cut(s) 94, 266, 304, 451, 488, 647, 739, 924
HpyCH4IV ACGT 1 cut(s) 818
HpyCH4V TGCA 7 cut(s) 4, 113, 426, 541, 638, 665, 857
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 981
HpyF3I CTNAG 5 cut(s) 295, 389, 400, 628, 902
HpySE526I ACGT 1 cut(s) 818
Hsp92I GRCGYC 1 cut(s) 525
Hsp92II CATG 4 cut(s) 30, 175, 592, 720
HspAI GCGC 1 cut(s) 599
Ksp22I TGATCA 2 cut(s) 348, 591
Kzo9I GATC 3 cut(s) 348, 591, 772
LmnI GCTCC 2 cut(s) 291, 1026
Lsp1109I GCAGC 2 cut(s) 438, 869
LweI GCATC 3 cut(s) 32, 207, 528
MaeI CTAG 2 cut(s) 120, 315
MaeII ACGT 1 cut(s) 818
MaeIII GTNAC 4 cut(s) 128, 521, 548, 960
MalI GATC 3 cut(s) 350, 593, 774
MboI GATC 3 cut(s) 348, 591, 772
MboII GAAGA 3 cut(s) 632, 760, 880
MhlI GDGCHC 3 cut(s) 296, 401, 1031
MlsI TGGCCA 1 cut(s) 334
MluCI AATT 5 cut(s) 473, 544, 671, 930, 948
MluNI TGGCCA 1 cut(s) 334
MlyI GAGTC 1 cut(s) 275
MmeI TCCRAC 2 cut(s) 253, 996
Mox20I TGGCCA 1 cut(s) 334
MroXI GAANNNNTTC 1 cut(s) 727
MscI TGGCCA 1 cut(s) 334
MseI TTAA 2 cut(s) 684, 876
MslI CAYNNNNRTG 2 cut(s) 201, 557
Msp20I TGGCCA 1 cut(s) 334
MspI CCGG 6 cut(s) 133, 168, 255, 421, 617, 668
MspR9I CCNGG 6 cut(s) 46, 377, 617, 852, 885, 986
MvaI CCWGG 5 cut(s) 46, 377, 852, 885, 986
MvnI CGCG 5 cut(s) 410, 483, 599, 609, 654
MwoI GCNNNNNNNGC 2 cut(s) 110, 981
NciI CCSGG 1 cut(s) 617
NcoI CCATGG 1 cut(s) 716
NdeII GATC 3 cut(s) 348, 591, 772
NlaIII CATG 4 cut(s) 30, 175, 592, 720
NlaIV GGNNCC 2 cut(s) 502, 1028
NmuCI GTSAC 4 cut(s) 128, 521, 548, 960
NruI TCGCGA 1 cut(s) 609
NspI RCATGY 1 cut(s) 175
PcsI WCGNNNNNNNCGW 1 cut(s) 651
PdmI GAANNNNTTC 1 cut(s) 727
PfeI GAWTC 4 cut(s) 31, 147, 193, 954
PflFI GACNNNGTC 1 cut(s) 449
PflMI CCANNNNNTGG 2 cut(s) 382, 964
PinAI ACCGGT 2 cut(s) 254, 420
PkrI GCNGC 2 cut(s) 428, 859
PleI GAGTC 1 cut(s) 275
PpsI GAGTC 1 cut(s) 275
Psp124BI GAGCTC 2 cut(s) 296, 401
Psp1406I AACGTT 1 cut(s) 818
Psp6I CCWGG 5 cut(s) 44, 375, 850, 883, 984
PspEI GGTNACC 1 cut(s) 128
PspGI CCWGG 5 cut(s) 44, 375, 850, 883, 984
PspN4I GGNNCC 2 cut(s) 502, 1028
PspPI GGNCC 4 cut(s) 361, 848, 898, 1067
PstNI CAGNNNCTG 1 cut(s) 964
PsyI GACNNNGTC 1 cut(s) 449
RruI TCGCGA 1 cut(s) 609
RsaI GTAC 2 cut(s) 176, 440
RsaNI GTAC 2 cut(s) 175, 439
RseI CAYNNNNRTG 2 cut(s) 201, 557
SacI GAGCTC 2 cut(s) 296, 401
SaqAI TTAA 2 cut(s) 684, 876
SatI GCNGC 2 cut(s) 427, 858
Sau3AI GATC 3 cut(s) 348, 591, 772
Sau96I GGNCC 4 cut(s) 361, 848, 898, 1067
SchI GAGTC 1 cut(s) 275
ScrFI CCNGG 6 cut(s) 46, 377, 617, 852, 885, 986
SduI GDGCHC 3 cut(s) 296, 401, 1031
SfaNI GCATC 3 cut(s) 32, 207, 528
SgrAI CRCCGGYG 1 cut(s) 132
SinI GGWCC 2 cut(s) 361, 898
SmiMI CAYNNNNRTG 2 cut(s) 201, 557
Sse9I AATT 5 cut(s) 473, 544, 671, 930, 948
SsiI CCGC 3 cut(s) 410, 481, 597
SspMI CTAG 2 cut(s) 120, 315
SstI GAGCTC 2 cut(s) 296, 401
StyD4I CCNGG 6 cut(s) 44, 375, 615, 850, 883, 984
StyI CCWWGG 1 cut(s) 716
TaaI ACNGT 8 cut(s) 94, 266, 304, 451, 488, 647, 739, 924
TaiI ACGT 1 cut(s) 821
TaqI TCGA 2 cut(s) 469, 507
TasI AATT 5 cut(s) 473, 544, 671, 930, 948
TfiI GAWTC 4 cut(s) 31, 147, 193, 954
Tru1I TTAA 2 cut(s) 684, 876
Tru9I TTAA 2 cut(s) 684, 876
TscAI CASTG 7 cut(s) 99, 111, 271, 387, 393, 454, 969
TseFI GTSAC 4 cut(s) 128, 521, 548, 960
TseI GCWGC 2 cut(s) 426, 857
Tsp45I GTSAC 4 cut(s) 128, 521, 548, 960
TspDTI ATGAA 6 cut(s) 185, 502, 587, 854, 881, 924
TspGWI ACGGA 1 cut(s) 24
TspRI CASTG 7 cut(s) 99, 111, 271, 387, 393, 454, 969
Tth111I GACNNNGTC 1 cut(s) 449
Van91I CCANNNNNTGG 2 cut(s) 382, 964
VpaK11BI GGWCC 2 cut(s) 361, 898
XapI RAATTY 1 cut(s) 671
XbaI TCTAGA 1 cut(s) 314
XceI RCATGY 1 cut(s) 175
XmnI GAANNNNTTC 1 cut(s) 727
XspI CTAG 2 cut(s) 120, 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.