Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1D

Orthogroup ID

OG0007373

Physical Location & Seq

Genomic Coordinates Forward (+)

42464606 .. 42466966

Gene Structure 2361 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1DG217500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1464 bp

ATGGCTATTGCTTCTTGCACCTCCTGCCTCCGAGATCCGAACCCGGTTCAGCTCGCCCTAAATGGCGTTCTTCAAGCCACCAATGTGGTCCGTACATCTATGGACCTGGTACATGGGTTGACAAGCTCCGAATCGGATTCTGCTGGCCATGATGTGGCTCTGATGGACTGTGAAAGACTATATGATGAGAGTGTGCCGAGGCTCACTGAGTTGATCTCCGGTGAGGGTAATTATACCAGAGAAGATGCTCGGACATGGCTCAGTGGTGTGCTTGCCAACAATAGGAGTTGTTGGGATGGCTTGGAGGAGAAGGGTTTTCTTAAGAATACTAGTGGTGCTCATCATTATAGGGCGGTGACACAAAACTTGACCATGGTGCTTGATGAAGCTCTTGCCTTGTATGGGAAGAGTACTATGACTATTGATGAGGCAATGAACGTACCACATGGACAAAACTCCAACCCCAGGCCAAATGGAGGGCTTTTAGCATCATGGAGTCCAGCAACATCCAGGGCTGACTTTGTGGTTGCAAAAGATGGTTCAGGCACTCACACAACACTTAACCAAGCCGTGGCTGCACTTGCTAGAATGGGTCACAGGCGACCTCCAAGAGCCATAATCTATGTGAAATCCGGCGTCTACAATGAGAAGGTGGAGATTGGTGGACATTTGAAGAATGTGATGCTTGTTGGGGATGGCATGGACATAACTGTAATCACCGGTAGCCGCAGTGTTCTGGACGGTGACAGCACTTTGACCTCAGCCACATTCGGTGTCTCAGGGGATGGTTTTTGGGCGAGTGACATAACGTTTGAGAATACGGCCAGTCCACAAAAGCACCAAGTAGTGGCATTGAGGGTCAGCTCTGACCTGGCAGTGTTTTACCGATGCAGCATTAAGGGGTACCAAGACACTCTCTACACCCATTCCCTAAGGCAATTCTACCGTGACTGTCACATATATGGCACAATCGATTTCATATTCGGTGACGCCCCCGTCGTGCTACAAAATTGCGACATTCTTGTGAGGAGGCCAATGGGGCACCAATCCAACATGATCACTGCCCAAGGACGCGACAACCCGGGAGAGAACACGGGCATTGTTATTCAAGGTTGTCGCATTAGACCTGCGAGGGAGTTTGAGGGGGTTAAGGGTTCGTATAGGACTTACCTTGGTCGGCCGTGGCAGAGATACTCGAGGACTCTGTTCTTGAAGACAGATTTGGATGGTTTGGTTGATCCCAAGGGGTGGACGGAATGGAGAGGTAGTTTTGCACTTTCAACACTGTATTACGGGGAGTATATGAATGTAGGGGTGGGTGCCTCAACTGATAAGAGGGTTAGATGGCCTGGTTTTCATGTGCTTAATAGTCCACAGGAGGTCAGCCCTTTCACAGTTAGTCGTTTCATTCAAGGAGAGTCATGGATCCTAGGCACTGGTGTGCCAGTTTGGCTTGGAATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

487

Amino Acids

53.52

Weight (kDa)

6.5

Isoelectric Point (pI)

36.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	6 - 128	3.6e-13	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	173 - 473	2.1e-131	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007373)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G60730
fragaria_vesca	FvH4_7g11333 FvH4_7g11340 FvH4_7g11360
malus_domestica	MD07G1118200.v1.1
prunus_persica	Prupe.2G141200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0349551 RchiOBHm_Chr1g0349581
rosa_laevigata	RLG00000028540 RLG00000028543
rosa_multiflora	Rmu_sc0002495.1_g000023 Rmu_sc0006074.1_g000009
rosa_roxburghii	Rroxscaffold_4G00305450
rosa_rugosa	Rorug01G0204500
rosa_samantha	Rh1AG221800 Rh1AG222000 Rh1BG187900 Rh1BG188200 Rh1CG206600 Rh1CG206900 Rh1DG217300 Rh1DG217500
rosa_wichuraiana	Rw0G014800 Rw1G018850 Rw1G018900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	995
Acc36I	ACCTGC	1 cut(s)	1135
Acc65I	GGTACC	1 cut(s)	903
AccB1I	GGYRCC	3 cut(s)	903, 1041, 1319
AccB7I	CCANNNNNTGG	4 cut(s)	154, 571, 847, 1248
AccI	GTMKAC	1 cut(s)	639
AccII	CGCG	1 cut(s)	1074
AciI	CCGC	2 cut(s)	353, 727
AclI	AACGTT	1 cut(s)	809
AclWI	GGATC	4 cut(s)	29, 1232, 1420, 1433
AcoI	YGGCCR	3 cut(s)	145, 822, 1178
AcyI	GRCGYC	2 cut(s)	636, 990
AfaI	GTAC	5 cut(s)	94, 111, 412, 441, 905
AfiI	CCNNNNNNNGG	8 cut(s)	154, 282, 402, 465, 476, 571, 847, 1248
AflII	CTTAAG	1 cut(s)	320
AgeI	ACCGGT	1 cut(s)	719
AgsI	TTSAA	6 cut(s)	74, 673, 1109, 1213, 1281, 1412
AhlI	ACTAGT	1 cut(s)	329
AjnI	CCWGG	5 cut(s)	105, 464, 509, 870, 1348
AjuI	GAANNNNNNNTTGG	2 cut(s)	1205, 1237
AleI	CACNNNNGTG	2 cut(s)	83, 1439
AluBI	AGCT	4 cut(s)	52, 126, 389, 864
AluI	AGCT	4 cut(s)	52, 126, 389, 864
Alw21I	GWGCWC	1 cut(s)	340
Alw26I	GTCTC	1 cut(s)	781
AlwI	GGATC	4 cut(s)	29, 1232, 1420, 1433
Ama87I	CYCGRG	2 cut(s)	1081, 1195
AoxI	GGCC	6 cut(s)	145, 467, 822, 1031, 1178, 1346
ApeKI	GCWGC	2 cut(s)	575, 891
ArsI	GACNNNNNNTTYG	2 cut(s)	794, 826
AsiGI	ACCGGT	1 cut(s)	719
Asp718I	GGTACC	1 cut(s)	903
AspA2I	CCTAGG	1 cut(s)	1429
AspS9I	GGNCC	2 cut(s)	88, 103
AsuC2I	CCSGG	3 cut(s)	44, 1082, 1083
AsuHPI	GGTGA	5 cut(s)	233, 367, 709, 755, 998
AvaI	CYCGRG	2 cut(s)	1081, 1195
AvaII	GGWCC	2 cut(s)	88, 103
AvrII	CCTAGG	1 cut(s)	1429
AxyI	CCTNAGG	1 cut(s)	932
BaeGI	GKGCMC	1 cut(s)	1044
BaeI	ACNNNNGTAYC	2 cut(s)	101, 134
BalI	TGGCCA	1 cut(s)	147
BamHI	GGATCC	1 cut(s)	1425
BanI	GGYRCC	3 cut(s)	903, 1041, 1319
BbsI	GAAGAC	1 cut(s)	1220
Bbv12I	GWGCWC	1 cut(s)	340
BbvCI	CCTCAGC	1 cut(s)	760
BbvI	GCAGC	2 cut(s)	562, 903
BccI	CCATC	7 cut(s)	157, 290, 530, 689, 779, 1220, 1338
BceAI	ACGGC	3 cut(s)	554, 837, 1165
BciT130I	CCWGG	5 cut(s)	107, 466, 511, 872, 1350
BclI	TGATCA	1 cut(s)	1056
BcnI	CCSGG	3 cut(s)	44, 1082, 1083
BcoDI	GTCTC	1 cut(s)	781
BcuI	ACTAGT	1 cut(s)	329
BfaI	CTAG	3 cut(s)	330, 585, 1430
BfrI	CTTAAG	1 cut(s)	320
BfuAI	ACCTGC	1 cut(s)	1135
BglI	GCCNNNNNGGC	2 cut(s)	1039, 1450
BisI	GCNGC	3 cut(s)	576, 727, 892
BlnI	CCTAGG	1 cut(s)	1429
BlsI	GCNGC	3 cut(s)	577, 728, 893
BmcAI	AGTACT	1 cut(s)	412
Bme1390I	CCNGG	8 cut(s)	44, 107, 466, 511, 872, 1082, 1083, 1350
Bme18I	GGWCC	2 cut(s)	88, 103
BmeT110I	CYCGRG	2 cut(s)	1081, 1195
BmgT120I	GGNCC	2 cut(s)	88, 103
BmiI	GGNNCC	4 cut(s)	905, 1043, 1321, 1427
BmrFI	CCNGG	8 cut(s)	44, 107, 466, 511, 872, 1082, 1083, 1350
BmsI	GCATC	4 cut(s)	235, 497, 672, 878
BpiI	GAAGAC	1 cut(s)	1220
BplI	GAGNNNNNCTC	2 cut(s)	200, 232
Bpu10I	CCTNAGC	1 cut(s)	760
BpuMI	CCSGG	3 cut(s)	44, 1082, 1083
Bsa29I	ATCGAT	1 cut(s)	972
BsaHI	GRCGYC	2 cut(s)	636, 990
BsaWI	WCCGGW	2 cut(s)	218, 719
BsaXI	ACNNNNNCTCC	2 cut(s)	1252, 1282
Bsc4I	CCNNNNNNNGG	8 cut(s)	154, 282, 402, 465, 476, 571, 847, 1248
Bse118I	RCCGGY	1 cut(s)	719
Bse1I	ACTGG	3 cut(s)	825, 1441, 1445
Bse21I	CCTNAGG	1 cut(s)	932
Bse3DI	GCAATG	1 cut(s)	438
BseBI	CCWGG	5 cut(s)	107, 466, 511, 872, 1350
BseCI	ATCGAT	1 cut(s)	972
BseGI	GGATG	5 cut(s)	301, 506, 700, 790, 1231
BseLI	CCNNNNNNNGG	8 cut(s)	154, 282, 402, 465, 476, 571, 847, 1248
BseMI	GCAATG	1 cut(s)	438
BseMII	CTCAG	4 cut(s)	198, 274, 774, 792
BseNI	ACTGG	3 cut(s)	825, 1441, 1445
BseRI	GAGGAG	2 cut(s)	320, 1042
BseSI	GKGCMC	1 cut(s)	1044
BseX3I	CGGCCG	1 cut(s)	1178
BseXI	GCAGC	2 cut(s)	562, 903
BsgI	GTGCAG	1 cut(s)	561
Bsh1236I	CGCG	1 cut(s)	1074
Bsh1285I	CGRYCG	1 cut(s)	1181
BshFI	GGCC	6 cut(s)	147, 469, 824, 1033, 1180, 1348
BshNI	GGYRCC	3 cut(s)	903, 1041, 1319
BshTI	ACCGGT	1 cut(s)	719
BshVI	ATCGAT	1 cut(s)	972
BsiEI	CGRYCG	1 cut(s)	1181
BsiHKAI	GWGCWC	1 cut(s)	340
BsiHKCI	CYCGRG	2 cut(s)	1081, 1195
BsiSI	CCGG	5 cut(s)	44, 219, 633, 720, 1082
BslI	CCNNNNNNNGG	8 cut(s)	154, 282, 402, 465, 476, 571, 847, 1248
BsmAI	GTCTC	1 cut(s)	781
BsnI	GGCC	6 cut(s)	147, 469, 824, 1033, 1180, 1348
BsoBI	CYCGRG	2 cut(s)	1081, 1195
Bsp1286I	GDGCHC	2 cut(s)	340, 1044
Bsp143I	GATC	5 cut(s)	34, 213, 1056, 1237, 1425
Bsp19I	CCATGG	1 cut(s)	372
BspACI	CCGC	2 cut(s)	353, 727
BspANI	GGCC	6 cut(s)	147, 469, 824, 1033, 1180, 1348
BspCNI	CTCAG	4 cut(s)	199, 273, 773, 791
BspDI	ATCGAT	1 cut(s)	972
BspFNI	CGCG	1 cut(s)	1074
BspLI	GGNNCC	4 cut(s)	905, 1043, 1321, 1427
BspMI	ACCTGC	1 cut(s)	1135
BspPI	GGATC	4 cut(s)	29, 1232, 1420, 1433
BspT107I	GGYRCC	3 cut(s)	903, 1041, 1319
BspTI	CTTAAG	1 cut(s)	320
BsrDI	GCAATG	1 cut(s)	438
BsrFI	RCCGGY	1 cut(s)	719
BsrI	ACTGG	3 cut(s)	825, 1441, 1445
BssAI	RCCGGY	1 cut(s)	719
BssMI	GATC	5 cut(s)	34, 213, 1056, 1237, 1425
BssNI	GRCGYC	2 cut(s)	636, 990
BssT1I	CCWWGG	5 cut(s)	372, 1066, 1171, 1242, 1429
Bst2UI	CCWGG	5 cut(s)	107, 466, 511, 872, 1350
Bst4CI	ACNGT	7 cut(s)	170, 712, 743, 947, 953, 1287, 1396
Bst6I	CTCTTC	1 cut(s)	401
BstACI	GRCGYC	2 cut(s)	636, 990
BstAFI	CTTAAG	1 cut(s)	320
BstAPI	GCANNNNNTGC	1 cut(s)	24
BstC8I	GCNNGC	3 cut(s)	54, 145, 273
BstDEI	CTNAG	5 cut(s)	207, 260, 760, 778, 932
BstDSI	CCRYGG	3 cut(s)	372, 570, 1181
BstF5I	GGATG	5 cut(s)	301, 506, 700, 790, 1231
BstFNI	CGCG	1 cut(s)	1074
BstKTI	GATC	5 cut(s)	37, 216, 1059, 1240, 1428
BstMAI	GTCTC	1 cut(s)	781
BstMBI	GATC	5 cut(s)	34, 213, 1056, 1237, 1425
BstMCI	CGRYCG	1 cut(s)	1181
BstMWI	GCNNNNNNNGC	5 cut(s)	24, 575, 581, 1039, 1450
BstNI	CCWGG	5 cut(s)	107, 466, 511, 872, 1350
BstSCI	CCNGG	8 cut(s)	42, 105, 464, 509, 870, 1080, 1081, 1348
BstSLI	GKGCMC	1 cut(s)	1044
BstUI	CGCG	1 cut(s)	1074
BstV1I	GCAGC	2 cut(s)	562, 903
BstV2I	GAAGAC	1 cut(s)	1220
BstX2I	RGATCY	2 cut(s)	34, 1425
BstXI	CCANNNNNNTGG	1 cut(s)	85
BstYI	RGATCY	2 cut(s)	34, 1425
BstZI	CGGCCG	1 cut(s)	1178
Bsu15I	ATCGAT	1 cut(s)	972
Bsu36I	CCTNAGG	1 cut(s)	932
BsuRI	GGCC	6 cut(s)	147, 469, 824, 1033, 1180, 1348
BsuTUI	ATCGAT	1 cut(s)	972
BtgI	CCRYGG	3 cut(s)	372, 570, 1181
BtsCI	GGATG	5 cut(s)	301, 506, 700, 790, 1231
BtsI	GCAGTG	3 cut(s)	736, 882, 1059
BtsIMutI	CAGTG	7 cut(s)	204, 268, 736, 882, 1059, 1283, 1434
BveI	ACCTGC	1 cut(s)	1135
Cac8I	GCNNGC	3 cut(s)	54, 145, 273
Cfr10I	RCCGGY	1 cut(s)	719
Cfr13I	GGNCC	2 cut(s)	88, 103
Cfr9I	CCCGGG	1 cut(s)	1081
ClaI	ATCGAT	1 cut(s)	972
CseI	GACGC	3 cut(s)	625, 998, 1080
CsiI	ACCWGGT	1 cut(s)	105
Csp6I	GTAC	5 cut(s)	93, 110, 411, 440, 904
CspAI	ACCGGT	1 cut(s)	719
CviQI	GTAC	5 cut(s)	93, 110, 411, 440, 904
DdeI	CTNAG	5 cut(s)	207, 260, 760, 778, 932
DpnI	GATC	5 cut(s)	36, 215, 1058, 1239, 1427
DpnII	GATC	5 cut(s)	34, 213, 1056, 1237, 1425
DrdI	GACNNNNNNGTC	1 cut(s)	995
DseDI	GACNNNNNNGTC	1 cut(s)	995
EaeI	YGGCCR	3 cut(s)	145, 822, 1178
EagI	CGGCCG	1 cut(s)	1178
Eam1104I	CTCTTC	1 cut(s)	401
EarI	CTCTTC	1 cut(s)	401
EclXI	CGGCCG	1 cut(s)	1178
Eco130I	CCWWGG	5 cut(s)	372, 1066, 1171, 1242, 1429
Eco47I	GGWCC	2 cut(s)	88, 103
Eco52I	CGGCCG	1 cut(s)	1178
Eco81I	CCTNAGG	1 cut(s)	932
Eco88I	CYCGRG	2 cut(s)	1081, 1195
EcoRII	CCWGG	5 cut(s)	105, 464, 509, 870, 1348
EcoT14I	CCWWGG	5 cut(s)	372, 1066, 1171, 1242, 1429
ErhI	CCWWGG	5 cut(s)	372, 1066, 1171, 1242, 1429
FbaI	TGATCA	1 cut(s)	1056
FblI	GTMKAC	1 cut(s)	639
Fnu4HI	GCNGC	3 cut(s)	576, 727, 892
FokI	GGATG	5 cut(s)	308, 493, 707, 797, 1238
Fsp4HI	GCNGC	3 cut(s)	576, 727, 892
FspBI	CTAG	3 cut(s)	330, 585, 1430
GluI	GCNGC	3 cut(s)	576, 727, 892
HaeIII	GGCC	6 cut(s)	147, 469, 824, 1033, 1180, 1348
HapII	CCGG	5 cut(s)	44, 219, 633, 720, 1082
HgaI	GACGC	3 cut(s)	625, 998, 1080
Hin1I	GRCGYC	2 cut(s)	636, 990
HincII	GTYRAC	1 cut(s)	120
HindII	GTYRAC	1 cut(s)	120
HinfI	GANTC	5 cut(s)	131, 137, 496, 1201, 1418
HpaII	CCGG	5 cut(s)	44, 219, 633, 720, 1082
HphI	GGTGA	5 cut(s)	233, 367, 709, 755, 998
Hpy166II	GTNNAC	6 cut(s)	120, 640, 665, 830, 1251, 1373
Hpy188I	TCNGA	7 cut(s)	32, 39, 130, 136, 162, 252, 868
Hpy188III	TCNNGA	2 cut(s)	737, 1210
Hpy8I	GTNNAC	6 cut(s)	120, 640, 665, 830, 1251, 1373
Hpy99I	CGWCG	1 cut(s)	1001
HpyAV	CCTTC	2 cut(s)	304, 643
HpyCH4III	ACNGT	7 cut(s)	170, 712, 743, 947, 953, 1287, 1396
HpyCH4IV	ACGT	2 cut(s)	438, 809
HpyCH4V	TGCA	5 cut(s)	18, 530, 578, 891, 1274
HpyF10VI	GCNNNNNNNGC	5 cut(s)	24, 575, 581, 1039, 1450
HpyF3I	CTNAG	5 cut(s)	207, 260, 760, 778, 932
HpySE526I	ACGT	2 cut(s)	438, 809
Hsp92I	GRCGYC	2 cut(s)	636, 990
KpnI	GGTACC	1 cut(s)	907
Ksp22I	TGATCA	1 cut(s)	1056
Kzo9I	GATC	5 cut(s)	34, 213, 1056, 1237, 1425
LmnI	GCTCC	1 cut(s)	131
Lsp1109I	GCAGC	2 cut(s)	562, 903
LweI	GCATC	4 cut(s)	235, 497, 672, 878
MabI	ACCWGGT	1 cut(s)	105
MaeI	CTAG	3 cut(s)	330, 585, 1430
MaeII	ACGT	2 cut(s)	438, 809
MaeIII	GTNAC	7 cut(s)	355, 593, 743, 800, 947, 953, 986
MalI	GATC	5 cut(s)	36, 215, 1058, 1239, 1427
MboI	GATC	5 cut(s)	34, 213, 1056, 1237, 1425
MboII	GAAGA	5 cut(s)	62, 254, 418, 685, 1225
MflI	RGATCY	2 cut(s)	34, 1425
MhlI	GDGCHC	2 cut(s)	340, 1044
MlsI	TGGCCA	1 cut(s)	147
MluCI	AATT	3 cut(s)	229, 938, 1009
MluNI	TGGCCA	1 cut(s)	147
MlyI	GAGTC	3 cut(s)	505, 1195, 1427
MmeI	TCCRAC	2 cut(s)	483, 1074
Mox20I	TGGCCA	1 cut(s)	147
MscI	TGGCCA	1 cut(s)	147
MseI	TTAA	5 cut(s)	321, 561, 897, 1149, 1365
MslI	CAYNNNNRTG	4 cut(s)	83, 960, 1022, 1439
Msp20I	TGGCCA	1 cut(s)	147
MspCI	CTTAAG	1 cut(s)	320
MspI	CCGG	5 cut(s)	44, 219, 633, 720, 1082
MspR9I	CCNGG	8 cut(s)	44, 107, 466, 511, 872, 1082, 1083, 1350
MvaI	CCWGG	5 cut(s)	107, 466, 511, 872, 1350
MvnI	CGCG	1 cut(s)	1074
MwoI	GCNNNNNNNGC	5 cut(s)	24, 575, 581, 1039, 1450
NciI	CCSGG	3 cut(s)	44, 1082, 1083
NcoI	CCATGG	1 cut(s)	372
NdeII	GATC	5 cut(s)	34, 213, 1056, 1237, 1425
NlaIV	GGNNCC	4 cut(s)	905, 1043, 1321, 1427
NmeAIII	GCCGAG	1 cut(s)	222
NmuCI	GTSAC	7 cut(s)	355, 593, 743, 800, 947, 953, 986
OliI	CACNNNNGTG	2 cut(s)	83, 1439
PaeR7I	CTCGAG	1 cut(s)	1195
PcsI	WCGNNNNNNNCGW	1 cut(s)	996
PfeI	GAWTC	2 cut(s)	131, 137
PflMI	CCANNNNNTGG	4 cut(s)	154, 571, 847, 1248
PinAI	ACCGGT	1 cut(s)	719
PkrI	GCNGC	3 cut(s)	577, 728, 893
PleI	GAGTC	3 cut(s)	504, 1195, 1426
PpsI	GAGTC	3 cut(s)	504, 1195, 1426
Psp1406I	AACGTT	1 cut(s)	809
Psp6I	CCWGG	5 cut(s)	105, 464, 509, 870, 1348
PspGI	CCWGG	5 cut(s)	105, 464, 509, 870, 1348
PspN4I	GGNNCC	4 cut(s)	905, 1043, 1321, 1427
PspPI	GGNCC	2 cut(s)	88, 103
PspXI	VCTCGAGB	1 cut(s)	1195
PsuI	RGATCY	2 cut(s)	34, 1425
RsaI	GTAC	5 cut(s)	94, 111, 412, 441, 905
RsaNI	GTAC	5 cut(s)	93, 110, 411, 440, 904
RseI	CAYNNNNRTG	4 cut(s)	83, 960, 1022, 1439
SaqAI	TTAA	5 cut(s)	321, 561, 897, 1149, 1365
SatI	GCNGC	3 cut(s)	576, 727, 892
Sau3AI	GATC	5 cut(s)	34, 213, 1056, 1237, 1425
Sau96I	GGNCC	2 cut(s)	88, 103
ScaI	AGTACT	1 cut(s)	412
SchI	GAGTC	3 cut(s)	505, 1195, 1427
ScrFI	CCNGG	8 cut(s)	44, 107, 466, 511, 872, 1082, 1083, 1350
SduI	GDGCHC	2 cut(s)	340, 1044
SexAI	ACCWGGT	1 cut(s)	105
SfaNI	GCATC	4 cut(s)	235, 497, 672, 878
Sfr274I	CTCGAG	1 cut(s)	1195
SinI	GGWCC	2 cut(s)	88, 103
SlaI	CTCGAG	1 cut(s)	1195
SmaI	CCCGGG	1 cut(s)	1083
SmiMI	CAYNNNNRTG	4 cut(s)	83, 960, 1022, 1439
SmlI	CTYRAG	2 cut(s)	320, 1195
SmoI	CTYRAG	2 cut(s)	320, 1195
SpeI	ACTAGT	1 cut(s)	329
Sse9I	AATT	3 cut(s)	229, 938, 1009
SsiI	CCGC	2 cut(s)	353, 727
SspMI	CTAG	3 cut(s)	330, 585, 1430
StyD4I	CCNGG	8 cut(s)	42, 105, 464, 509, 870, 1080, 1081, 1348
StyI	CCWWGG	5 cut(s)	372, 1066, 1171, 1242, 1429
TaaI	ACNGT	7 cut(s)	170, 712, 743, 947, 953, 1287, 1396
TaiI	ACGT	2 cut(s)	441, 812
TaqI	TCGA	2 cut(s)	972, 1196
TasI	AATT	3 cut(s)	229, 938, 1009
TatI	WGTACW	1 cut(s)	410
TauI	GCSGC	1 cut(s)	729
TfiI	GAWTC	2 cut(s)	131, 137
Tru1I	TTAA	5 cut(s)	321, 561, 897, 1149, 1365
Tru9I	TTAA	5 cut(s)	321, 561, 897, 1149, 1365
TscAI	CASTG	7 cut(s)	211, 268, 736, 882, 1066, 1290, 1441
TseFI	GTSAC	7 cut(s)	355, 593, 743, 800, 947, 953, 986
TseI	GCWGC	2 cut(s)	575, 891
Tsp45I	GTSAC	7 cut(s)	355, 593, 743, 800, 947, 953, 986
TspDTI	ATGAA	6 cut(s)	399, 449, 967, 1319, 1346, 1396
TspGWI	ACGGA	2 cut(s)	80, 1268
TspMI	CCCGGG	1 cut(s)	1081
TspRI	CASTG	7 cut(s)	211, 268, 736, 882, 1066, 1290, 1441
Van91I	CCANNNNNTGG	4 cut(s)	154, 571, 847, 1248
Vha464I	CTTAAG	1 cut(s)	320
VpaK11BI	GGWCC	2 cut(s)	88, 103
XhoI	CTCGAG	1 cut(s)	1195
XmaI	CCCGGG	1 cut(s)	1081
XmaJI	CCTAGG	1 cut(s)	1429
XmiI	GTMKAC	1 cut(s)	639
XspI	CTAG	3 cut(s)	330, 585, 1430
ZrmI	AGTACT	1 cut(s)	412

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.