Rw1G018900

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0007373
Physical Location & Seq
Forward (+)
41466075 .. 41467378
1304 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G018900.1

Sequence Viewer

Length: 636 bp
ATGGACCTGGTACATGGGTTGACAAGCTCCGAATCGGATTCTGCTGGCCATGATGTGGCTCTGATGGATTGTGAAAGACTATATGATGAGAGTGTGCCGAGGCTCACTGAGTTGATCTCCGGTGAGGGAGTTGTTGGGATGGCTTGGAGGAGAAGGGTTTTCTTAATTAAGAATACTAGTGGTGCTCATCATTATAGGGCGGTGACACAAAACTTGACCATGGTGCTTGATGAGGCTCTTGCTTTGTATGGGAAGAACGTGCCACATGGACAAAACTCCAACCCCAGGTCAAATGGAGGGCTTTTAGCATCATGGAGTCCAGCAACATCCAGGGCTGACTTTGTGGTTGCAAAAGATGGTTCAGGCACTCACACAACACTTAACCAAGCCGTGGCTGCACTTGCTAGAATGGGTCACAGGCGACCTCCAAGAGCCATAATCTATGTGAAATCCGGAGTCTACAATGAGAAGGTGGAGATTGGTGGACATATGAAGAATGTGATACTTGTTGGGGATGGAATGGACAGAACTGTAATCACTGGTAGCCGCAGTGTCGTGGACGGTGACAGCACTTTGACCTCAGCCACATTCGGTGAGTTTGATCCCAAAAATCAATCATTATATATTCCAAATTAA

Protein Analysis

211

Amino Acids

22.76

Weight (kDa)

6.7

Isoelectric Point (pI)

34.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 113 - 198 6e-23 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 55, 391
AccI GTMKAC 1 cut(s) 459
AccIII TCCGGA 1 cut(s) 452
AciI CCGC 2 cut(s) 200, 547
AclWI GGATC 1 cut(s) 596
AcoI YGGCCR 1 cut(s) 46
AfaI GTAC 1 cut(s) 12
AfiI CCNNNNNNNGG 3 cut(s) 55, 285, 391
AhlI ACTAGT 1 cut(s) 176
AjnI CCWGG 3 cut(s) 6, 284, 329
AluBI AGCT 1 cut(s) 27
AluI AGCT 1 cut(s) 27
Alw21I GWGCWC 1 cut(s) 187
AlwI GGATC 1 cut(s) 596
Aor13HI TCCGGA 1 cut(s) 452
AoxI GGCC 1 cut(s) 46
ApeKI GCWGC 1 cut(s) 395
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 4 cut(s) 134, 214, 575, 605
AvaII GGWCC 1 cut(s) 4
BaeI ACNNNNGTAYC 1 cut(s) 35
BalI TGGCCA 1 cut(s) 48
Bbv12I GWGCWC 1 cut(s) 187
BbvCI CCTCAGC 1 cut(s) 580
BbvI GCAGC 1 cut(s) 382
BccI CCATC 4 cut(s) 58, 133, 350, 509
BceAI ACGGC 1 cut(s) 374
BciT130I CCWGG 3 cut(s) 8, 286, 331
BcuI ACTAGT 1 cut(s) 176
BfaI CTAG 2 cut(s) 177, 405
BisI GCNGC 2 cut(s) 396, 547
BlsI GCNGC 2 cut(s) 397, 548
Bme1390I CCNGG 3 cut(s) 8, 286, 331
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 1 cut(s) 4
BmrFI CCNGG 3 cut(s) 8, 286, 331
BmsI GCATC 1 cut(s) 317
BplI GAGNNNNNCTC 2 cut(s) 101, 133
Bpu10I CCTNAGC 1 cut(s) 580
BsaJI CCNNGG 5 cut(s) 98, 219, 284, 330, 390
BsaWI WCCGGW 2 cut(s) 119, 452
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 285, 391
Bse1I ACTGG 1 cut(s) 544
BseAI TCCGGA 1 cut(s) 452
BseBI CCWGG 3 cut(s) 8, 286, 331
BseDI CCNNGG 5 cut(s) 98, 219, 284, 330, 390
BseGI GGATG 3 cut(s) 144, 326, 520
BseLI CCNNNNNNNGG 3 cut(s) 55, 285, 391
BseMII CTCAG 2 cut(s) 99, 594
BseNI ACTGG 1 cut(s) 544
BseRI GAGGAG 1 cut(s) 163
BseXI GCAGC 1 cut(s) 382
BsgI GTGCAG 1 cut(s) 381
BshFI GGCC 1 cut(s) 48
BsiHKAI GWGCWC 1 cut(s) 187
BsiSI CCGG 2 cut(s) 120, 453
BslI CCNNNNNNNGG 3 cut(s) 55, 285, 391
BsnI GGCC 1 cut(s) 48
Bsp1286I GDGCHC 1 cut(s) 187
Bsp13I TCCGGA 1 cut(s) 452
Bsp143I GATC 2 cut(s) 114, 601
Bsp19I CCATGG 1 cut(s) 219
BspACI CCGC 2 cut(s) 200, 547
BspANI GGCC 1 cut(s) 48
BspCNI CTCAG 2 cut(s) 100, 593
BspEI TCCGGA 1 cut(s) 452
BspPI GGATC 1 cut(s) 596
BsrI ACTGG 1 cut(s) 544
BssECI CCNNGG 5 cut(s) 98, 219, 284, 330, 390
BssMI GATC 2 cut(s) 114, 601
BssT1I CCWWGG 1 cut(s) 219
Bst2UI CCWGG 3 cut(s) 8, 286, 331
Bst4CI ACNGT 2 cut(s) 532, 563
BstC8I GCNNGC 1 cut(s) 46
BstDEI CTNAG 2 cut(s) 108, 580
BstDSI CCRYGG 2 cut(s) 219, 390
BstF5I GGATG 3 cut(s) 144, 326, 520
BstKTI GATC 2 cut(s) 117, 604
BstMBI GATC 2 cut(s) 114, 601
BstMWI GCNNNNNNNGC 2 cut(s) 395, 401
BstNI CCWGG 3 cut(s) 8, 286, 331
BstSCI CCNGG 3 cut(s) 6, 284, 329
BstV1I GCAGC 1 cut(s) 382
BsuRI GGCC 1 cut(s) 48
BtgI CCRYGG 2 cut(s) 219, 390
BtsCI GGATG 3 cut(s) 144, 326, 520
BtsI GCAGTG 1 cut(s) 556
BtsIMutI CAGTG 3 cut(s) 105, 537, 556
Cac8I GCNNGC 1 cut(s) 46
Cfr13I GGNCC 1 cut(s) 4
CsiI ACCWGGT 1 cut(s) 6
Csp6I GTAC 1 cut(s) 11
CviAII CATG 5 cut(s) 14, 50, 220, 266, 312
CviQI GTAC 1 cut(s) 11
DdeI CTNAG 2 cut(s) 108, 580
DpnI GATC 2 cut(s) 116, 603
DpnII GATC 2 cut(s) 114, 601
EaeI YGGCCR 1 cut(s) 46
Eco130I CCWWGG 1 cut(s) 219
Eco47I GGWCC 1 cut(s) 4
EcoRII CCWGG 3 cut(s) 6, 284, 329
EcoT14I CCWWGG 1 cut(s) 219
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 5 cut(s) 17, 53, 223, 269, 315
FatI CATG 5 cut(s) 13, 49, 219, 265, 311
FauNDI CATATG 1 cut(s) 489
FblI GTMKAC 1 cut(s) 459
Fnu4HI GCNGC 2 cut(s) 396, 547
FokI GGATG 3 cut(s) 151, 313, 527
Fsp4HI GCNGC 2 cut(s) 396, 547
FspBI CTAG 2 cut(s) 177, 405
GluI GCNGC 2 cut(s) 396, 547
HaeIII GGCC 1 cut(s) 48
HapII CCGG 2 cut(s) 120, 453
Hin1II CATG 5 cut(s) 17, 53, 223, 269, 315
HincII GTYRAC 1 cut(s) 21
HindII GTYRAC 1 cut(s) 21
HinfI GANTC 4 cut(s) 32, 38, 316, 456
HpaII CCGG 2 cut(s) 120, 453
HphI GGTGA 4 cut(s) 134, 214, 575, 605
Hpy166II GTNNAC 4 cut(s) 21, 460, 485, 559
Hpy188I TCNGA 3 cut(s) 31, 37, 63
Hpy188III TCNNGA 1 cut(s) 453
Hpy8I GTNNAC 4 cut(s) 21, 460, 485, 559
HpyAV CCTTC 2 cut(s) 147, 463
HpyCH4III ACNGT 2 cut(s) 532, 563
HpyCH4IV ACGT 1 cut(s) 258
HpyCH4V TGCA 2 cut(s) 350, 398
HpyF10VI GCNNNNNNNGC 2 cut(s) 395, 401
HpyF3I CTNAG 2 cut(s) 108, 580
HpySE526I ACGT 1 cut(s) 258
Hsp92II CATG 5 cut(s) 17, 53, 223, 269, 315
Kpn2I TCCGGA 1 cut(s) 452
Kzo9I GATC 2 cut(s) 114, 601
LmnI GCTCC 1 cut(s) 32
Lsp1109I GCAGC 1 cut(s) 382
LweI GCATC 1 cut(s) 317
MabI ACCWGGT 1 cut(s) 6
MaeI CTAG 2 cut(s) 177, 405
MaeII ACGT 1 cut(s) 258
MaeIII GTNAC 3 cut(s) 202, 413, 563
MalI GATC 2 cut(s) 116, 603
MboI GATC 2 cut(s) 114, 601
MboII GAAGA 2 cut(s) 265, 505
MhlI GDGCHC 1 cut(s) 187
MlsI TGGCCA 1 cut(s) 48
MluCI AATT 2 cut(s) 165, 631
MluNI TGGCCA 1 cut(s) 48
MlyI GAGTC 2 cut(s) 325, 465
MmeI TCCRAC 1 cut(s) 303
MnlI CCTC 7 cut(s) 93, 118, 141, 226, 290, 435, 589
Mox20I TGGCCA 1 cut(s) 48
MroI TCCGGA 1 cut(s) 452
MscI TGGCCA 1 cut(s) 48
MseI TTAA 4 cut(s) 164, 168, 381, 634
Msp20I TGGCCA 1 cut(s) 48
MspI CCGG 2 cut(s) 120, 453
MspR9I CCNGG 3 cut(s) 8, 286, 331
MvaI CCWGG 3 cut(s) 8, 286, 331
MwoI GCNNNNNNNGC 2 cut(s) 395, 401
NcoI CCATGG 1 cut(s) 219
NdeI CATATG 1 cut(s) 489
NdeII GATC 2 cut(s) 114, 601
NlaIII CATG 5 cut(s) 17, 53, 223, 269, 315
NmeAIII GCCGAG 1 cut(s) 123
NmuCI GTSAC 3 cut(s) 202, 413, 563
PacI TTAATTAA 1 cut(s) 168
PfeI GAWTC 2 cut(s) 32, 38
PflMI CCANNNNNTGG 2 cut(s) 55, 391
PkrI GCNGC 2 cut(s) 397, 548
PleI GAGTC 2 cut(s) 324, 464
PpsI GAGTC 2 cut(s) 324, 464
Psp6I CCWGG 3 cut(s) 6, 284, 329
PspGI CCWGG 3 cut(s) 6, 284, 329
PspPI GGNCC 1 cut(s) 4
RsaI GTAC 1 cut(s) 12
RsaNI GTAC 1 cut(s) 11
SaqAI TTAA 4 cut(s) 164, 168, 381, 634
SatI GCNGC 2 cut(s) 396, 547
Sau3AI GATC 2 cut(s) 114, 601
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 2 cut(s) 325, 465
ScrFI CCNGG 3 cut(s) 8, 286, 331
SduI GDGCHC 1 cut(s) 187
SetI ASST 7 cut(s) 9, 29, 261, 290, 427, 474, 581
SexAI ACCWGGT 1 cut(s) 6
SfaNI GCATC 1 cut(s) 317
SinI GGWCC 1 cut(s) 4
SpeI ACTAGT 1 cut(s) 176
Sse9I AATT 2 cut(s) 165, 631
SsiI CCGC 2 cut(s) 200, 547
SspMI CTAG 2 cut(s) 177, 405
StyD4I CCNGG 3 cut(s) 6, 284, 329
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 2 cut(s) 532, 563
TaiI ACGT 1 cut(s) 261
TasI AATT 2 cut(s) 165, 631
TauI GCSGC 1 cut(s) 549
TfiI GAWTC 2 cut(s) 32, 38
Tru1I TTAA 4 cut(s) 164, 168, 381, 634
Tru9I TTAA 4 cut(s) 164, 168, 381, 634
TscAI CASTG 3 cut(s) 112, 544, 556
TseFI GTSAC 3 cut(s) 202, 413, 563
TseI GCWGC 1 cut(s) 395
Tsp45I GTSAC 3 cut(s) 202, 413, 563
TspDTI ATGAA 1 cut(s) 506
TspRI CASTG 3 cut(s) 112, 544, 556
Van91I CCANNNNNTGG 2 cut(s) 55, 391
VpaK11BI GGWCC 1 cut(s) 4
XmiI GTMKAC 1 cut(s) 459
XspI CTAG 2 cut(s) 177, 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.