Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0007373

Physical Location & Seq

Genomic Coordinates Forward (+)

42655248 .. 42657162

Gene Structure 1915 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ57555

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1107 bp

ATGGACTGTGAAAGACTATATGATGAGAGTGTGCCGAGGCTCACTGAGTTGATCTCCGGTGAGCGTAATTATACCAGAGAAGATGCTCGGACATGGCTCAGTGGTGTGCTTGCCAACCATAGGAGTTGTTGGGATGGCTTGGAGGAGAAGGGTTTTCTTAAGAATGCTAGTGGTGCTCATCATTATAGGGCGGTGACACAAAACTTGACCATGGTGCTTGATGAGGCTCTTGCTTTGTATGGGAAGAGTACTATGACTACTGATGAGGCAATGAACGTATCACATGGACAAAACTCCAAACCCAGGCCAAATGGAGGGCTTTTAGCATCATGGAGTCCAGCAACATCCAGGGCTGACTTCGTGGTTGCAAAAGATGGTTCAGGCACTCACACAACACTTAACCAAGCCGTGGCTGCACTTGCTAGAATGGGTCACAGACGACCTCCAAGAGCCATAATCTATGTGAAATCCGGCGTCTACAATGAGAAGGTGGAAATTGGTGGACATATGAAGAATGTGATGCTTGTTGGGGATGGCATGGACAGAACTGTAATCACTGGTAGCCGCAGTGTCATGGACGGTGACAGCACTTTGACCTCAGCCACATTCGGTGTCTCCGGGGATGGTTTCTGGGCGAGGGACATAACGTTTGAGAATACGGCCGGTCCACAAAAGCACCAAGCAGTGGCATTGAGGGTCAGCTCTGACCTGGCAGTGTTTTACCGATGCAGCATTAAGGGGTACCAAGACACTCTCTACACCCATTCCCTAAGGCAATTCTACAGTGACTGTCACATATATGGCACAATTGATTTCATATTCGGTGACGCCCCCGTCGTGCTACAAAATTGTGACATTCTTGTGAGGAGGCCAATGGGGCACCAATCCAACATGATCACTGCCCAAGGACGCGACAACCCGGGAGAGAACACGGGCATTGTTATTCAAGGTTGTAGCGTTAGACCTGCGAGGGAGTTTGAGGGGGTTAAGGGTTCGTATAGGACTTACCTTGGTCGGCCGTGGCAGAGATACTCGAGGACTCTGTTCTTGAAGACAGATTTGGATGGTTTGGTTGATCCAAAGGGGTGGACGGAATGGAGAGGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

368

Amino Acids

40.8

Weight (kDa)

8.16

Isoelectric Point (pI)

29.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	2 - 74	3.8e-09	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	119 - 368	4.6e-111	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007373)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G60730
fragaria_vesca	FvH4_7g11333 FvH4_7g11340 FvH4_7g11360
malus_domestica	MD07G1118200.v1.1
prunus_persica	Prupe.2G141200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0349551 RchiOBHm_Chr1g0349581
rosa_laevigata	RLG00000028540 RLG00000028543
rosa_multiflora	Rmu_sc0002495.1_g000023 Rmu_sc0006074.1_g000009
rosa_roxburghii	Rroxscaffold_4G00305450
rosa_rugosa	Rorug01G0204500
rosa_samantha	Rh1AG221800 Rh1AG222000 Rh1BG187900 Rh1BG188200 Rh1CG206600 Rh1CG206900 Rh1DG217300 Rh1DG217500
rosa_wichuraiana	Rw0G014800 Rw1G018850 Rw1G018900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	833
Acc36I	ACCTGC	1 cut(s)	973
Acc65I	GGTACC	1 cut(s)	741
AccB1I	GGYRCC	2 cut(s)	741, 879
AccB7I	CCANNNNNTGG	2 cut(s)	409, 685
AccI	GTMKAC	1 cut(s)	477
AccII	CGCG	1 cut(s)	912
AciI	CCGC	2 cut(s)	191, 565
AclI	AACGTT	1 cut(s)	647
AclWI	GGATC	1 cut(s)	1070
AcoI	YGGCCR	2 cut(s)	660, 1016
AcyI	GRCGYC	2 cut(s)	474, 828
AfaI	GTAC	2 cut(s)	250, 743
AfiI	CCNNNNNNNGG	5 cut(s)	120, 303, 314, 409, 685
AflII	CTTAAG	1 cut(s)	158
AgsI	TTSAA	2 cut(s)	947, 1051
AjnI	CCWGG	3 cut(s)	302, 347, 708
AjuI	GAANNNNNNNTTGG	2 cut(s)	1043, 1075
AluBI	AGCT	1 cut(s)	702
AluI	AGCT	1 cut(s)	702
Alw21I	GWGCWC	1 cut(s)	178
Alw26I	GTCTC	1 cut(s)	619
AlwI	GGATC	1 cut(s)	1070
AlwNI	CAGNNNCTG	1 cut(s)	789
Ama87I	CYCGRG	2 cut(s)	919, 1033
AoxI	GGCC	4 cut(s)	305, 660, 869, 1016
ApeKI	GCWGC	2 cut(s)	413, 729
ArsI	GACNNNNNNTTYG	2 cut(s)	632, 664
Asp718I	GGTACC	1 cut(s)	741
AspS9I	GGNCC	1 cut(s)	665
AsuC2I	CCSGG	3 cut(s)	619, 920, 921
AsuHPI	GGTGA	4 cut(s)	71, 205, 593, 836
AvaI	CYCGRG	2 cut(s)	919, 1033
AvaII	GGWCC	1 cut(s)	665
AxyI	CCTNAGG	1 cut(s)	770
BaeGI	GKGCMC	1 cut(s)	882
BanI	GGYRCC	2 cut(s)	741, 879
BbsI	GAAGAC	1 cut(s)	1058
Bbv12I	GWGCWC	1 cut(s)	178
BbvCI	CCTCAGC	1 cut(s)	598
BbvI	GCAGC	2 cut(s)	400, 741
BccI	CCATC	5 cut(s)	128, 368, 527, 617, 1058
BceAI	ACGGC	3 cut(s)	392, 675, 1003
BciT130I	CCWGG	3 cut(s)	304, 349, 710
BclI	TGATCA	1 cut(s)	894
BcnI	CCSGG	3 cut(s)	619, 920, 921
BcoDI	GTCTC	1 cut(s)	619
BfaI	CTAG	3 cut(s)	168, 423, 1105
BfmI	CTRYAG	1 cut(s)	781
BfrI	CTTAAG	1 cut(s)	158
BfuAI	ACCTGC	1 cut(s)	973
BglI	GCCNNNNNGGC	1 cut(s)	877
BisI	GCNGC	3 cut(s)	414, 565, 730
BlsI	GCNGC	3 cut(s)	415, 566, 731
BmcAI	AGTACT	1 cut(s)	250
Bme1390I	CCNGG	6 cut(s)	304, 349, 619, 710, 920, 921
Bme18I	GGWCC	1 cut(s)	665
BmeT110I	CYCGRG	2 cut(s)	919, 1033
BmgT120I	GGNCC	1 cut(s)	665
BmiI	GGNNCC	2 cut(s)	743, 881
BmrFI	CCNGG	6 cut(s)	304, 349, 619, 710, 920, 921
BmsI	GCATC	4 cut(s)	73, 335, 510, 716
BpiI	GAAGAC	1 cut(s)	1058
BplI	GAGNNNNNCTC	2 cut(s)	38, 70
Bpu10I	CCTNAGC	1 cut(s)	598
BpuMI	CCSGG	3 cut(s)	619, 920, 921
BsaHI	GRCGYC	2 cut(s)	474, 828
BsaWI	WCCGGW	1 cut(s)	56
Bsc4I	CCNNNNNNNGG	5 cut(s)	120, 303, 314, 409, 685
Bse118I	RCCGGY	1 cut(s)	662
Bse1I	ACTGG	1 cut(s)	562
Bse21I	CCTNAGG	1 cut(s)	770
Bse3DI	GCAATG	1 cut(s)	276
BseBI	CCWGG	3 cut(s)	304, 349, 710
BseGI	GGATG	5 cut(s)	139, 344, 538, 628, 1069
BseLI	CCNNNNNNNGG	5 cut(s)	120, 303, 314, 409, 685
BseMI	GCAATG	1 cut(s)	276
BseMII	CTCAG	3 cut(s)	36, 112, 612
BseNI	ACTGG	1 cut(s)	562
BseRI	GAGGAG	2 cut(s)	158, 880
BseSI	GKGCMC	1 cut(s)	882
BseX3I	CGGCCG	2 cut(s)	660, 1016
BseXI	GCAGC	2 cut(s)	400, 741
BsgI	GTGCAG	1 cut(s)	399
Bsh1236I	CGCG	1 cut(s)	912
Bsh1285I	CGRYCG	2 cut(s)	663, 1019
BshFI	GGCC	4 cut(s)	307, 662, 871, 1018
BshNI	GGYRCC	2 cut(s)	741, 879
BsiEI	CGRYCG	2 cut(s)	663, 1019
BsiHKAI	GWGCWC	1 cut(s)	178
BsiHKCI	CYCGRG	2 cut(s)	919, 1033
BsiSI	CCGG	5 cut(s)	57, 471, 618, 663, 920
BslFI	GGGAC	1 cut(s)	653
BslI	CCNNNNNNNGG	5 cut(s)	120, 303, 314, 409, 685
BsmAI	GTCTC	1 cut(s)	619
BsmFI	GGGAC	1 cut(s)	653
BsmI	GAATGC	1 cut(s)	169
BsnI	GGCC	4 cut(s)	307, 662, 871, 1018
BsoBI	CYCGRG	2 cut(s)	919, 1033
Bsp1286I	GDGCHC	2 cut(s)	178, 882
Bsp143I	GATC	3 cut(s)	51, 894, 1075
Bsp19I	CCATGG	1 cut(s)	210
BspACI	CCGC	2 cut(s)	191, 565
BspANI	GGCC	4 cut(s)	307, 662, 871, 1018
BspCNI	CTCAG	3 cut(s)	37, 111, 611
BspFNI	CGCG	1 cut(s)	912
BspLI	GGNNCC	2 cut(s)	743, 881
BspMI	ACCTGC	1 cut(s)	973
BspPI	GGATC	1 cut(s)	1070
BspT107I	GGYRCC	2 cut(s)	741, 879
BspTI	CTTAAG	1 cut(s)	158
BsrDI	GCAATG	1 cut(s)	276
BsrFI	RCCGGY	1 cut(s)	662
BsrI	ACTGG	1 cut(s)	562
BssAI	RCCGGY	1 cut(s)	662
BssMI	GATC	3 cut(s)	51, 894, 1075
BssNI	GRCGYC	2 cut(s)	474, 828
BssT1I	CCWWGG	3 cut(s)	210, 904, 1009
Bst2UI	CCWGG	3 cut(s)	304, 349, 710
Bst4CI	ACNGT	5 cut(s)	8, 550, 581, 785, 791
Bst6I	CTCTTC	1 cut(s)	239
BstACI	GRCGYC	2 cut(s)	474, 828
BstAFI	CTTAAG	1 cut(s)	158
BstC8I	GCNNGC	1 cut(s)	111
BstDEI	CTNAG	4 cut(s)	45, 98, 598, 770
BstDSI	CCRYGG	3 cut(s)	210, 408, 1019
BstF5I	GGATG	5 cut(s)	139, 344, 538, 628, 1069
BstFNI	CGCG	1 cut(s)	912
BstKTI	GATC	3 cut(s)	54, 897, 1078
BstMAI	GTCTC	1 cut(s)	619
BstMBI	GATC	3 cut(s)	51, 894, 1075
BstMCI	CGRYCG	2 cut(s)	663, 1019
BstMWI	GCNNNNNNNGC	4 cut(s)	173, 413, 419, 877
BstNI	CCWGG	3 cut(s)	304, 349, 710
BstSCI	CCNGG	6 cut(s)	302, 347, 617, 708, 918, 919
BstSFI	CTRYAG	1 cut(s)	781
BstSLI	GKGCMC	1 cut(s)	882
BstUI	CGCG	1 cut(s)	912
BstV1I	GCAGC	2 cut(s)	400, 741
BstV2I	GAAGAC	1 cut(s)	1058
BstXI	CCANNNNNNTGG	1 cut(s)	1086
BstZI	CGGCCG	2 cut(s)	660, 1016
Bsu36I	CCTNAGG	1 cut(s)	770
BsuRI	GGCC	4 cut(s)	307, 662, 871, 1018
BtgI	CCRYGG	3 cut(s)	210, 408, 1019
BtsCI	GGATG	5 cut(s)	139, 344, 538, 628, 1069
BtsI	GCAGTG	4 cut(s)	574, 690, 720, 897
BtsIMutI	CAGTG	8 cut(s)	42, 106, 555, 574, 690, 720, 790, 897
BveI	ACCTGC	1 cut(s)	973
Cac8I	GCNNGC	1 cut(s)	111
CaiI	CAGNNNCTG	1 cut(s)	789
Cfr10I	RCCGGY	1 cut(s)	662
Cfr13I	GGNCC	1 cut(s)	665
Cfr9I	CCCGGG	1 cut(s)	919
CseI	GACGC	3 cut(s)	463, 836, 918
Csp6I	GTAC	2 cut(s)	249, 742
CviAII	CATG	7 cut(s)	93, 211, 284, 330, 538, 574, 892
CviQI	GTAC	2 cut(s)	249, 742
DdeI	CTNAG	4 cut(s)	45, 98, 598, 770
DpnI	GATC	3 cut(s)	53, 896, 1077
DpnII	GATC	3 cut(s)	51, 894, 1075
DrdI	GACNNNNNNGTC	1 cut(s)	833
DseDI	GACNNNNNNGTC	1 cut(s)	833
EaeI	YGGCCR	2 cut(s)	660, 1016
EagI	CGGCCG	2 cut(s)	660, 1016
Eam1104I	CTCTTC	1 cut(s)	239
EarI	CTCTTC	1 cut(s)	239
EclXI	CGGCCG	2 cut(s)	660, 1016
Eco130I	CCWWGG	3 cut(s)	210, 904, 1009
Eco47I	GGWCC	1 cut(s)	665
Eco52I	CGGCCG	2 cut(s)	660, 1016
Eco81I	CCTNAGG	1 cut(s)	770
Eco88I	CYCGRG	2 cut(s)	919, 1033
EcoRII	CCWGG	3 cut(s)	302, 347, 708
EcoT14I	CCWWGG	3 cut(s)	210, 904, 1009
ErhI	CCWWGG	3 cut(s)	210, 904, 1009
FaeI	CATG	7 cut(s)	96, 214, 287, 333, 541, 577, 895
FaqI	GGGAC	1 cut(s)	653
FatI	CATG	7 cut(s)	92, 210, 283, 329, 537, 573, 891
FauNDI	CATATG	1 cut(s)	507
FbaI	TGATCA	1 cut(s)	894
FblI	GTMKAC	1 cut(s)	477
Fnu4HI	GCNGC	3 cut(s)	414, 565, 730
FokI	GGATG	5 cut(s)	146, 331, 545, 635, 1076
Fsp4HI	GCNGC	3 cut(s)	414, 565, 730
FspBI	CTAG	3 cut(s)	168, 423, 1105
GluI	GCNGC	3 cut(s)	414, 565, 730
HaeIII	GGCC	4 cut(s)	307, 662, 871, 1018
HapII	CCGG	5 cut(s)	57, 471, 618, 663, 920
HgaI	GACGC	3 cut(s)	463, 836, 918
Hin1I	GRCGYC	2 cut(s)	474, 828
Hin1II	CATG	7 cut(s)	96, 214, 287, 333, 541, 577, 895
HinfI	GANTC	2 cut(s)	334, 1039
HpaII	CCGG	5 cut(s)	57, 471, 618, 663, 920
HphI	GGTGA	4 cut(s)	71, 205, 593, 836
Hpy166II	GTNNAC	4 cut(s)	478, 503, 668, 1089
Hpy188I	TCNGA	2 cut(s)	90, 706
Hpy188III	TCNNGA	1 cut(s)	1048
Hpy8I	GTNNAC	4 cut(s)	478, 503, 668, 1089
Hpy99I	CGWCG	1 cut(s)	839
HpyAV	CCTTC	2 cut(s)	142, 481
HpyCH4III	ACNGT	5 cut(s)	8, 550, 581, 785, 791
HpyCH4IV	ACGT	2 cut(s)	276, 647
HpyCH4V	TGCA	3 cut(s)	368, 416, 729
HpyF10VI	GCNNNNNNNGC	4 cut(s)	173, 413, 419, 877
HpyF3I	CTNAG	4 cut(s)	45, 98, 598, 770
HpySE526I	ACGT	2 cut(s)	276, 647
Hsp92I	GRCGYC	2 cut(s)	474, 828
Hsp92II	CATG	7 cut(s)	96, 214, 287, 333, 541, 577, 895
KpnI	GGTACC	1 cut(s)	745
Ksp22I	TGATCA	1 cut(s)	894
Kzo9I	GATC	3 cut(s)	51, 894, 1075
Lsp1109I	GCAGC	2 cut(s)	400, 741
LweI	GCATC	4 cut(s)	73, 335, 510, 716
MaeI	CTAG	3 cut(s)	168, 423, 1105
MaeII	ACGT	2 cut(s)	276, 647
MaeIII	GTNAC	7 cut(s)	193, 431, 581, 785, 791, 824, 851
MalI	GATC	3 cut(s)	53, 896, 1077
MboI	GATC	3 cut(s)	51, 894, 1075
MboII	GAAGA	4 cut(s)	92, 256, 523, 1063
MfeI	CAATTG	1 cut(s)	807
MhlI	GDGCHC	2 cut(s)	178, 882
MluCI	AATT	5 cut(s)	67, 495, 776, 807, 847
MlyI	GAGTC	2 cut(s)	343, 1033
MmeI	TCCRAC	1 cut(s)	912
MseI	TTAA	4 cut(s)	159, 399, 735, 987
MslI	CAYNNNNRTG	2 cut(s)	798, 860
MspCI	CTTAAG	1 cut(s)	158
MspI	CCGG	5 cut(s)	57, 471, 618, 663, 920
MspR9I	CCNGG	6 cut(s)	304, 349, 619, 710, 920, 921
MunI	CAATTG	1 cut(s)	807
Mva1269I	GAATGC	1 cut(s)	169
MvaI	CCWGG	3 cut(s)	304, 349, 710
MvnI	CGCG	1 cut(s)	912
MwoI	GCNNNNNNNGC	4 cut(s)	173, 413, 419, 877
NciI	CCSGG	3 cut(s)	619, 920, 921
NcoI	CCATGG	1 cut(s)	210
NdeI	CATATG	1 cut(s)	507
NdeII	GATC	3 cut(s)	51, 894, 1075
NlaIII	CATG	7 cut(s)	96, 214, 287, 333, 541, 577, 895
NlaIV	GGNNCC	2 cut(s)	743, 881
NmeAIII	GCCGAG	1 cut(s)	60
NmuCI	GTSAC	7 cut(s)	193, 431, 581, 785, 791, 824, 851
PaeR7I	CTCGAG	1 cut(s)	1033
PcsI	WCGNNNNNNNCGW	1 cut(s)	834
PctI	GAATGC	1 cut(s)	169
PflMI	CCANNNNNTGG	2 cut(s)	409, 685
PkrI	GCNGC	3 cut(s)	415, 566, 731
PleI	GAGTC	2 cut(s)	342, 1033
PpsI	GAGTC	2 cut(s)	342, 1033
Psp1406I	AACGTT	1 cut(s)	647
Psp6I	CCWGG	3 cut(s)	302, 347, 708
PspGI	CCWGG	3 cut(s)	302, 347, 708
PspN4I	GGNNCC	2 cut(s)	743, 881
PspPI	GGNCC	1 cut(s)	665
PspXI	VCTCGAGB	1 cut(s)	1033
PstNI	CAGNNNCTG	1 cut(s)	789
RsaI	GTAC	2 cut(s)	250, 743
RsaNI	GTAC	2 cut(s)	249, 742
RseI	CAYNNNNRTG	2 cut(s)	798, 860
SaqAI	TTAA	4 cut(s)	159, 399, 735, 987
SatI	GCNGC	3 cut(s)	414, 565, 730
Sau3AI	GATC	3 cut(s)	51, 894, 1075
Sau96I	GGNCC	1 cut(s)	665
ScaI	AGTACT	1 cut(s)	250
SchI	GAGTC	2 cut(s)	343, 1033
ScrFI	CCNGG	6 cut(s)	304, 349, 619, 710, 920, 921
SduI	GDGCHC	2 cut(s)	178, 882
SfaNI	GCATC	4 cut(s)	73, 335, 510, 716
SfcI	CTRYAG	1 cut(s)	781
Sfr274I	CTCGAG	1 cut(s)	1033
SinI	GGWCC	1 cut(s)	665
SlaI	CTCGAG	1 cut(s)	1033
SmaI	CCCGGG	1 cut(s)	921
SmiMI	CAYNNNNRTG	2 cut(s)	798, 860
SmlI	CTYRAG	2 cut(s)	158, 1033
SmoI	CTYRAG	2 cut(s)	158, 1033
Sse9I	AATT	5 cut(s)	67, 495, 776, 807, 847
SsiI	CCGC	2 cut(s)	191, 565
SspMI	CTAG	3 cut(s)	168, 423, 1105
StyD4I	CCNGG	6 cut(s)	302, 347, 617, 708, 918, 919
StyI	CCWWGG	3 cut(s)	210, 904, 1009
TaaI	ACNGT	5 cut(s)	8, 550, 581, 785, 791
TaiI	ACGT	2 cut(s)	279, 650
TaqI	TCGA	1 cut(s)	1034
TasI	AATT	5 cut(s)	67, 495, 776, 807, 847
TatI	WGTACW	1 cut(s)	248
TauI	GCSGC	1 cut(s)	567
Tru1I	TTAA	4 cut(s)	159, 399, 735, 987
Tru9I	TTAA	4 cut(s)	159, 399, 735, 987
TscAI	CASTG	8 cut(s)	49, 106, 562, 574, 690, 720, 790, 904
TseFI	GTSAC	7 cut(s)	193, 431, 581, 785, 791, 824, 851
TseI	GCWGC	2 cut(s)	413, 729
Tsp45I	GTSAC	7 cut(s)	193, 431, 581, 785, 791, 824, 851
TspDTI	ATGAA	3 cut(s)	287, 524, 805
TspGWI	ACGGA	1 cut(s)	1106
TspMI	CCCGGG	1 cut(s)	919
TspRI	CASTG	8 cut(s)	49, 106, 562, 574, 690, 720, 790, 904
Van91I	CCANNNNNTGG	2 cut(s)	409, 685
Vha464I	CTTAAG	1 cut(s)	158
VpaK11BI	GGWCC	1 cut(s)	665
XhoI	CTCGAG	1 cut(s)	1033
XmaI	CCCGGG	1 cut(s)	919
XmiI	GTMKAC	1 cut(s)	477
XspI	CTAG	3 cut(s)	168, 423, 1105
ZrmI	AGTACT	1 cut(s)	250

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.