Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0007373

Physical Location & Seq

Genomic Coordinates Reverse (-)

24061433 .. 24063704

Gene Structure 2272 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000028540

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1557 bp

ATGTACACTTCTGTCACCACCTTCCTCCTGCTAGCCATGGCTATTGCTTCTTGCACCTCCTGCTTCCGAGATCCGAACCCGGTTCAGCTCGCCCTAAATGGCGTTCTTCAAGCCACCAATGTGGTCCGTACATCTATGGACCTGGTACATGGGTTGACAAGCTCCGAATCGGATTCCGCTGGCCATAATGCGGCTCTGATGGACTGTGAAAGACTATATGATGAGAGTGTGCCGAGGCTCACTGAGTTGATCTCCGGTGAGGGTAATTATACCAGAGAAGATGCTCGGACATGGCTCAGTGGGGTGCTTGCCAACCATAGGAGTTGTTGGGATGGCTTGCAGGAGAAGGGTTTTCTTAAGAATACTAGTGGTGCTCATCATTATAGGGCGGTGACACAAAACTTGACCATGGTGCTTGATGAAGCTCTTGCTTTGTATGGGAAGAGTACTATGACTACTGATGAGGCAATGATGATTCAAATTGCTATCCCAACTGAGTTTTTGCAATTTATGGTGTTATATATAGTAGACGTACCACATGGACAAAACTCCAAACCCAGGCCAAATGGAGGGCTTTTAGCATCATGGAGTCCAGCAACATCCAGGGCTGACTTTGTGGTTGCAAAAGATGGTTCAGGCACTCACACAACACTTAACCAAGCCGTGGCTGCACTTGCTAGAATGGGTCACAGGCGACCTCCAAGAGCCATAATCTATGTGAAATCCGGCGTCTACAATGAGAAGGTGGAGATTGGTGGACAGATGAAGAATGTGATGCTTGTTGGGGATGGCATGGACAGAACTGTAGTCACTGGTAGCCGCAGTGTCGTGGACGGTGACAGCACTTTGAACTCAGCCACATTCGGTGTCTCAGGGGATGGTTTCTGGGTGAGGGACATAACGTTTGAGAATACGGCCGGTCCACAAAAGCACCAAGCCGTGGCATTGAGGGTCAGCTCTGACCTGGCAGTGTTTTACCGATGCAGCATTAAGGGGTACCAAGACACTCTCTACACCCATTCCCTAAGGCAATTCTACCGTGACTGTCACATATATGGCACAATCGATTTCATATTCGGTGACGCCCCCGTCGTGCTACAAAATTGTGACATTCTTGTGAGGAGGCCAATGGGGCACCAATCCAACATGATCACTGCCCAAGGACGCGACAACCTGGGAGAGAACACGGGCATTGTTATTCAAGGTTGTCGCGTTAGACCTGCGAGGGAGTTTGAGGGGGTTAAGGGTTCGTATAGGACTTACCTTGGTCGGCCGTGGCAGAGATACTCGAGGACTCTGTTCTTGAAGACAGATTTGGATGGTTTGGTTGATCCAAAGGGGTGGACAGAATGGAGAGGTAGTTTTGCACTTTCAACACTGTATTATGGGGAGTATATGAATGTAGGGGTGGGTGCCTCAACTGACAAGAGGGTTAGATGGCCTGGTTTTCATGTGCTTAGTAGTCCACAGGAGGTCAGCCCTTTCACAGTTAGTCGTTTCATTCAAGGAGAGTCATGGATTCTAGGCATTGGTGTGCCAGTTTGGCTTGGAATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

519

Amino Acids

57.2

Weight (kDa)

7.2

Isoelectric Point (pI)

34.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	20 - 140	1.1e-13	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	204 - 504	3.9e-134	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007373)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G60730
fragaria_vesca	FvH4_7g11333 FvH4_7g11340 FvH4_7g11360
malus_domestica	MD07G1118200.v1.1
prunus_persica	Prupe.2G141200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0349551 RchiOBHm_Chr1g0349581
rosa_laevigata	RLG00000028540 RLG00000028543
rosa_multiflora	Rmu_sc0002495.1_g000023 Rmu_sc0006074.1_g000009
rosa_roxburghii	Rroxscaffold_4G00305450
rosa_rugosa	Rorug01G0204500
rosa_samantha	Rh1AG221800 Rh1AG222000 Rh1BG187900 Rh1BG188200 Rh1CG206600 Rh1CG206900 Rh1DG217300 Rh1DG217500
rosa_wichuraiana	Rw0G014800 Rw1G018850 Rw1G018900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1088
Acc36I	ACCTGC	1 cut(s)	1228
Acc65I	GGTACC	1 cut(s)	996
AccB1I	GGYRCC	3 cut(s)	996, 1134, 1412
AccB7I	CCANNNNNTGG	2 cut(s)	664, 940
AccI	GTMKAC	2 cut(s)	528, 732
AccII	CGCG	2 cut(s)	1167, 1212
AciI	CCGC	4 cut(s)	177, 191, 389, 820
AclI	AACGTT	1 cut(s)	902
AclWI	GGATC	2 cut(s)	65, 1325
AcoI	YGGCCR	3 cut(s)	181, 915, 1271
AcyI	GRCGYC	2 cut(s)	729, 1083
AfaI	GTAC	6 cut(s)	5, 130, 147, 448, 534, 998
AfiI	CCNNNNNNNGG	6 cut(s)	190, 318, 558, 569, 664, 940
AflII	CTTAAG	1 cut(s)	356
AgsI	TTSAA	7 cut(s)	110, 479, 850, 1202, 1306, 1374, 1505
AhlI	ACTAGT	1 cut(s)	365
AjnI	CCWGG	6 cut(s)	141, 557, 602, 963, 1173, 1441
AjuI	GAANNNNNNNTTGG	2 cut(s)	1298, 1330
AleI	CACNNNNGTG	1 cut(s)	119
AluBI	AGCT	4 cut(s)	88, 162, 425, 957
AluI	AGCT	4 cut(s)	88, 162, 425, 957
Alw21I	GWGCWC	1 cut(s)	376
Alw26I	GTCTC	1 cut(s)	874
AlwI	GGATC	2 cut(s)	65, 1325
Ama87I	CYCGRG	1 cut(s)	1288
AoxI	GGCC	6 cut(s)	181, 560, 915, 1124, 1271, 1439
ApeKI	GCWGC	2 cut(s)	668, 984
ArsI	GACNNNNNNTTYG	2 cut(s)	887, 919
Asp718I	GGTACC	1 cut(s)	996
AspS9I	GGNCC	3 cut(s)	124, 139, 920
AsuC2I	CCSGG	1 cut(s)	80
AsuHPI	GGTGA	6 cut(s)	7, 269, 403, 848, 901, 1091
AsuNHI	GCTAGC	1 cut(s)	31
AvaI	CYCGRG	1 cut(s)	1288
AvaII	GGWCC	3 cut(s)	124, 139, 920
AxyI	CCTNAGG	1 cut(s)	1025
BaeGI	GKGCMC	1 cut(s)	1137
BaeI	ACNNNNGTAYC	2 cut(s)	137, 170
BalI	TGGCCA	1 cut(s)	183
BanI	GGYRCC	3 cut(s)	996, 1134, 1412
BbsI	GAAGAC	1 cut(s)	1313
Bbv12I	GWGCWC	1 cut(s)	376
BbvI	GCAGC	2 cut(s)	655, 996
BccI	CCATC	7 cut(s)	193, 326, 623, 782, 872, 1313, 1431
BceAI	ACGGC	4 cut(s)	647, 923, 930, 1258
BciT130I	CCWGG	6 cut(s)	143, 559, 604, 965, 1175, 1443
BclI	TGATCA	1 cut(s)	1149
BcnI	CCSGG	1 cut(s)	80
BcoDI	GTCTC	1 cut(s)	874
BcuI	ACTAGT	1 cut(s)	365
BfaI	CTAG	4 cut(s)	32, 366, 678, 1523
BfmI	CTRYAG	1 cut(s)	804
BfrI	CTTAAG	1 cut(s)	356
BfuAI	ACCTGC	1 cut(s)	1228
BglI	GCCNNNNNGGC	2 cut(s)	1132, 1543
BisI	GCNGC	4 cut(s)	192, 669, 820, 985
BlsI	GCNGC	4 cut(s)	193, 670, 821, 986
BmcAI	AGTACT	1 cut(s)	448
Bme1390I	CCNGG	7 cut(s)	80, 143, 559, 604, 965, 1175, 1443
Bme18I	GGWCC	3 cut(s)	124, 139, 920
BmeT110I	CYCGRG	1 cut(s)	1288
BmgT120I	GGNCC	3 cut(s)	124, 139, 920
BmiI	GGNNCC	3 cut(s)	998, 1136, 1414
BmrFI	CCNGG	7 cut(s)	80, 143, 559, 604, 965, 1175, 1443
BmsI	GCATC	4 cut(s)	271, 590, 765, 971
BmtI	GCTAGC	1 cut(s)	35
BpiI	GAAGAC	1 cut(s)	1313
BplI	GAGNNNNNCTC	2 cut(s)	236, 268
BpuMI	CCSGG	1 cut(s)	80
Bsa29I	ATCGAT	1 cut(s)	1065
BsaHI	GRCGYC	2 cut(s)	729, 1083
BsaWI	WCCGGW	1 cut(s)	254
BsaXI	ACNNNNNCTCC	2 cut(s)	1345, 1375
Bsc4I	CCNNNNNNNGG	6 cut(s)	190, 318, 558, 569, 664, 940
Bse118I	RCCGGY	1 cut(s)	917
Bse1I	ACTGG	2 cut(s)	817, 1538
Bse21I	CCTNAGG	1 cut(s)	1025
Bse3DI	GCAATG	1 cut(s)	474
BseBI	CCWGG	6 cut(s)	143, 559, 604, 965, 1175, 1443
BseCI	ATCGAT	1 cut(s)	1065
BseGI	GGATG	5 cut(s)	337, 599, 793, 883, 1324
BseLI	CCNNNNNNNGG	6 cut(s)	190, 318, 558, 569, 664, 940
BseMI	GCAATG	1 cut(s)	474
BseMII	CTCAG	5 cut(s)	234, 310, 486, 867, 885
BseNI	ACTGG	2 cut(s)	817, 1538
BseRI	GAGGAG	1 cut(s)	1135
BseSI	GKGCMC	1 cut(s)	1137
BseX3I	CGGCCG	2 cut(s)	915, 1271
BseXI	GCAGC	2 cut(s)	655, 996
BsgI	GTGCAG	1 cut(s)	654
Bsh1236I	CGCG	2 cut(s)	1167, 1212
Bsh1285I	CGRYCG	2 cut(s)	918, 1274
BshFI	GGCC	6 cut(s)	183, 562, 917, 1126, 1273, 1441
BshNI	GGYRCC	3 cut(s)	996, 1134, 1412
BshVI	ATCGAT	1 cut(s)	1065
BsiEI	CGRYCG	2 cut(s)	918, 1274
BsiHKAI	GWGCWC	1 cut(s)	376
BsiHKCI	CYCGRG	1 cut(s)	1288
BsiSI	CCGG	4 cut(s)	80, 255, 726, 918
BslFI	GGGAC	1 cut(s)	908
BslI	CCNNNNNNNGG	6 cut(s)	190, 318, 558, 569, 664, 940
BsmAI	GTCTC	1 cut(s)	874
BsmFI	GGGAC	1 cut(s)	908
BsnI	GGCC	6 cut(s)	183, 562, 917, 1126, 1273, 1441
BsoBI	CYCGRG	1 cut(s)	1288
Bsp1286I	GDGCHC	2 cut(s)	376, 1137
Bsp1407I	TGTACA	1 cut(s)	3
Bsp143I	GATC	4 cut(s)	70, 249, 1149, 1330
Bsp19I	CCATGG	2 cut(s)	36, 408
BspACI	CCGC	4 cut(s)	177, 191, 389, 820
BspANI	GGCC	6 cut(s)	183, 562, 917, 1126, 1273, 1441
BspCNI	CTCAG	5 cut(s)	235, 309, 487, 866, 884
BspDI	ATCGAT	1 cut(s)	1065
BspFNI	CGCG	2 cut(s)	1167, 1212
BspLI	GGNNCC	3 cut(s)	998, 1136, 1414
BspMI	ACCTGC	1 cut(s)	1228
BspOI	GCTAGC	1 cut(s)	35
BspPI	GGATC	2 cut(s)	65, 1325
BspT107I	GGYRCC	3 cut(s)	996, 1134, 1412
BspTI	CTTAAG	1 cut(s)	356
BsrDI	GCAATG	1 cut(s)	474
BsrFI	RCCGGY	1 cut(s)	917
BsrGI	TGTACA	1 cut(s)	3
BsrI	ACTGG	2 cut(s)	817, 1538
BssAI	RCCGGY	1 cut(s)	917
BssMI	GATC	4 cut(s)	70, 249, 1149, 1330
BssNI	GRCGYC	2 cut(s)	729, 1083
BssT1I	CCWWGG	4 cut(s)	36, 408, 1159, 1264
Bst2UI	CCWGG	6 cut(s)	143, 559, 604, 965, 1175, 1443
Bst4CI	ACNGT	7 cut(s)	206, 805, 836, 1040, 1046, 1380, 1489
Bst6I	CTCTTC	1 cut(s)	437
BstACI	GRCGYC	2 cut(s)	729, 1083
BstAFI	CTTAAG	1 cut(s)	356
BstAPI	GCANNNNNTGC	1 cut(s)	60
BstAUI	TGTACA	1 cut(s)	3
BstC8I	GCNNGC	5 cut(s)	33, 90, 181, 309, 338
BstDEI	CTNAG	7 cut(s)	243, 296, 495, 853, 871, 1025, 1457
BstDSI	CCRYGG	5 cut(s)	36, 408, 663, 939, 1274
BstF5I	GGATG	5 cut(s)	337, 599, 793, 883, 1324
BstFNI	CGCG	2 cut(s)	1167, 1212
BstKTI	GATC	4 cut(s)	73, 252, 1152, 1333
BstMAI	GTCTC	1 cut(s)	874
BstMBI	GATC	4 cut(s)	70, 249, 1149, 1330
BstMCI	CGRYCG	2 cut(s)	918, 1274
BstMWI	GCNNNNNNNGC	5 cut(s)	60, 668, 674, 1132, 1543
BstNI	CCWGG	6 cut(s)	143, 559, 604, 965, 1175, 1443
BstSCI	CCNGG	7 cut(s)	78, 141, 557, 602, 963, 1173, 1441
BstSFI	CTRYAG	1 cut(s)	804
BstSLI	GKGCMC	1 cut(s)	1137
BstUI	CGCG	2 cut(s)	1167, 1212
BstV1I	GCAGC	2 cut(s)	655, 996
BstV2I	GAAGAC	1 cut(s)	1313
BstX2I	RGATCY	1 cut(s)	70
BstXI	CCANNNNNNTGG	2 cut(s)	121, 1341
BstYI	RGATCY	1 cut(s)	70
BstZI	CGGCCG	2 cut(s)	915, 1271
Bsu15I	ATCGAT	1 cut(s)	1065
Bsu36I	CCTNAGG	1 cut(s)	1025
BsuRI	GGCC	6 cut(s)	183, 562, 917, 1126, 1273, 1441
BsuTUI	ATCGAT	1 cut(s)	1065
BtgI	CCRYGG	5 cut(s)	36, 408, 663, 939, 1274
BtsCI	GGATG	5 cut(s)	337, 599, 793, 883, 1324
BtsI	GCAGTG	3 cut(s)	829, 975, 1152
BtsIMutI	CAGTG	7 cut(s)	240, 304, 810, 829, 975, 1152, 1376
BveI	ACCTGC	1 cut(s)	1228
Cac8I	GCNNGC	5 cut(s)	33, 90, 181, 309, 338
Cfr10I	RCCGGY	1 cut(s)	917
Cfr13I	GGNCC	3 cut(s)	124, 139, 920
ClaI	ATCGAT	1 cut(s)	1065
CseI	GACGC	3 cut(s)	718, 1091, 1173
CsiI	ACCWGGT	1 cut(s)	141
Csp6I	GTAC	6 cut(s)	4, 129, 146, 447, 533, 997
CviQI	GTAC	6 cut(s)	4, 129, 146, 447, 533, 997
DdeI	CTNAG	7 cut(s)	243, 296, 495, 853, 871, 1025, 1457
DpnI	GATC	4 cut(s)	72, 251, 1151, 1332
DpnII	GATC	4 cut(s)	70, 249, 1149, 1330
DrdI	GACNNNNNNGTC	1 cut(s)	1088
DseDI	GACNNNNNNGTC	1 cut(s)	1088
EaeI	YGGCCR	3 cut(s)	181, 915, 1271
EagI	CGGCCG	2 cut(s)	915, 1271
Eam1104I	CTCTTC	1 cut(s)	437
EarI	CTCTTC	1 cut(s)	437
EclXI	CGGCCG	2 cut(s)	915, 1271
Eco130I	CCWWGG	4 cut(s)	36, 408, 1159, 1264
Eco47I	GGWCC	3 cut(s)	124, 139, 920
Eco52I	CGGCCG	2 cut(s)	915, 1271
Eco81I	CCTNAGG	1 cut(s)	1025
Eco88I	CYCGRG	1 cut(s)	1288
EcoRII	CCWGG	6 cut(s)	141, 557, 602, 963, 1173, 1441
EcoT14I	CCWWGG	4 cut(s)	36, 408, 1159, 1264
ErhI	CCWWGG	4 cut(s)	36, 408, 1159, 1264
FaqI	GGGAC	1 cut(s)	908
FbaI	TGATCA	1 cut(s)	1149
FblI	GTMKAC	2 cut(s)	528, 732
Fnu4HI	GCNGC	4 cut(s)	192, 669, 820, 985
FokI	GGATG	5 cut(s)	344, 586, 800, 890, 1331
Fsp4HI	GCNGC	4 cut(s)	192, 669, 820, 985
FspBI	CTAG	4 cut(s)	32, 366, 678, 1523
GluI	GCNGC	4 cut(s)	192, 669, 820, 985
HaeIII	GGCC	6 cut(s)	183, 562, 917, 1126, 1273, 1441
HapII	CCGG	4 cut(s)	80, 255, 726, 918
HgaI	GACGC	3 cut(s)	718, 1091, 1173
Hin1I	GRCGYC	2 cut(s)	729, 1083
HincII	GTYRAC	1 cut(s)	156
HindII	GTYRAC	1 cut(s)	156
HinfI	GANTC	7 cut(s)	167, 173, 475, 589, 1294, 1511, 1519
HpaII	CCGG	4 cut(s)	80, 255, 726, 918
HphI	GGTGA	6 cut(s)	7, 269, 403, 848, 901, 1091
Hpy166II	GTNNAC	9 cut(s)	6, 156, 529, 733, 758, 832, 923, 1344, 1466
Hpy188I	TCNGA	7 cut(s)	68, 75, 166, 172, 198, 288, 961
Hpy188III	TCNNGA	1 cut(s)	1303
Hpy8I	GTNNAC	9 cut(s)	6, 156, 529, 733, 758, 832, 923, 1344, 1466
Hpy99I	CGWCG	1 cut(s)	1094
HpyAV	CCTTC	3 cut(s)	31, 340, 736
HpyCH4III	ACNGT	7 cut(s)	206, 805, 836, 1040, 1046, 1380, 1489
HpyCH4IV	ACGT	2 cut(s)	531, 902
HpyCH4V	TGCA	7 cut(s)	54, 340, 505, 623, 671, 984, 1367
HpyF10VI	GCNNNNNNNGC	5 cut(s)	60, 668, 674, 1132, 1543
HpyF3I	CTNAG	7 cut(s)	243, 296, 495, 853, 871, 1025, 1457
HpySE526I	ACGT	2 cut(s)	531, 902
Hsp92I	GRCGYC	2 cut(s)	729, 1083
KpnI	GGTACC	1 cut(s)	1000
Ksp22I	TGATCA	1 cut(s)	1149
Kzo9I	GATC	4 cut(s)	70, 249, 1149, 1330
LmnI	GCTCC	1 cut(s)	167
Lsp1109I	GCAGC	2 cut(s)	655, 996
LweI	GCATC	4 cut(s)	271, 590, 765, 971
MabI	ACCWGGT	1 cut(s)	141
MaeI	CTAG	4 cut(s)	32, 366, 678, 1523
MaeII	ACGT	2 cut(s)	531, 902
MaeIII	GTNAC	9 cut(s)	13, 391, 686, 808, 836, 1040, 1046, 1079, 1106
MalI	GATC	4 cut(s)	72, 251, 1151, 1332
MboI	GATC	4 cut(s)	70, 249, 1149, 1330
MboII	GAAGA	5 cut(s)	98, 290, 454, 778, 1318
MflI	RGATCY	1 cut(s)	70
MhlI	GDGCHC	2 cut(s)	376, 1137
MlsI	TGGCCA	1 cut(s)	183
MluCI	AATT	5 cut(s)	265, 480, 506, 1031, 1102
MluNI	TGGCCA	1 cut(s)	183
MlyI	GAGTC	3 cut(s)	598, 1288, 1520
MmeI	TCCRAC	1 cut(s)	1167
Mox20I	TGGCCA	1 cut(s)	183
MscI	TGGCCA	1 cut(s)	183
MseI	TTAA	4 cut(s)	357, 654, 990, 1242
MslI	CAYNNNNRTG	4 cut(s)	119, 1053, 1115, 1532
Msp20I	TGGCCA	1 cut(s)	183
MspA1I	CMGCKG	1 cut(s)	179
MspCI	CTTAAG	1 cut(s)	356
MspI	CCGG	4 cut(s)	80, 255, 726, 918
MspR9I	CCNGG	7 cut(s)	80, 143, 559, 604, 965, 1175, 1443
MvaI	CCWGG	6 cut(s)	143, 559, 604, 965, 1175, 1443
MvnI	CGCG	2 cut(s)	1167, 1212
MwoI	GCNNNNNNNGC	5 cut(s)	60, 668, 674, 1132, 1543
NciI	CCSGG	1 cut(s)	80
NcoI	CCATGG	2 cut(s)	36, 408
NdeII	GATC	4 cut(s)	70, 249, 1149, 1330
NheI	GCTAGC	1 cut(s)	31
NlaIV	GGNNCC	3 cut(s)	998, 1136, 1414
NmeAIII	GCCGAG	1 cut(s)	258
NmuCI	GTSAC	9 cut(s)	13, 391, 686, 808, 836, 1040, 1046, 1079, 1106
OliI	CACNNNNGTG	1 cut(s)	119
PaeR7I	CTCGAG	1 cut(s)	1288
PcsI	WCGNNNNNNNCGW	1 cut(s)	1089
PfeI	GAWTC	4 cut(s)	167, 173, 475, 1519
PflMI	CCANNNNNTGG	2 cut(s)	664, 940
PkrI	GCNGC	4 cut(s)	193, 670, 821, 986
PleI	GAGTC	3 cut(s)	597, 1288, 1519
PpsI	GAGTC	3 cut(s)	597, 1288, 1519
Psp1406I	AACGTT	1 cut(s)	902
Psp6I	CCWGG	6 cut(s)	141, 557, 602, 963, 1173, 1441
PspGI	CCWGG	6 cut(s)	141, 557, 602, 963, 1173, 1441
PspN4I	GGNNCC	3 cut(s)	998, 1136, 1414
PspPI	GGNCC	3 cut(s)	124, 139, 920
PspXI	VCTCGAGB	1 cut(s)	1288
PsuI	RGATCY	1 cut(s)	70
RsaI	GTAC	6 cut(s)	5, 130, 147, 448, 534, 998
RsaNI	GTAC	6 cut(s)	4, 129, 146, 447, 533, 997
RseI	CAYNNNNRTG	4 cut(s)	119, 1053, 1115, 1532
SaqAI	TTAA	4 cut(s)	357, 654, 990, 1242
SatI	GCNGC	4 cut(s)	192, 669, 820, 985
Sau3AI	GATC	4 cut(s)	70, 249, 1149, 1330
Sau96I	GGNCC	3 cut(s)	124, 139, 920
ScaI	AGTACT	1 cut(s)	448
SchI	GAGTC	3 cut(s)	598, 1288, 1520
ScrFI	CCNGG	7 cut(s)	80, 143, 559, 604, 965, 1175, 1443
SduI	GDGCHC	2 cut(s)	376, 1137
SexAI	ACCWGGT	1 cut(s)	141
SfaNI	GCATC	4 cut(s)	271, 590, 765, 971
SfcI	CTRYAG	1 cut(s)	804
Sfr274I	CTCGAG	1 cut(s)	1288
SinI	GGWCC	3 cut(s)	124, 139, 920
SlaI	CTCGAG	1 cut(s)	1288
SmiMI	CAYNNNNRTG	4 cut(s)	119, 1053, 1115, 1532
SmlI	CTYRAG	2 cut(s)	356, 1288
SmoI	CTYRAG	2 cut(s)	356, 1288
SpeI	ACTAGT	1 cut(s)	365
Sse9I	AATT	5 cut(s)	265, 480, 506, 1031, 1102
SsiI	CCGC	4 cut(s)	177, 191, 389, 820
SspMI	CTAG	4 cut(s)	32, 366, 678, 1523
StyD4I	CCNGG	7 cut(s)	78, 141, 557, 602, 963, 1173, 1441
StyI	CCWWGG	4 cut(s)	36, 408, 1159, 1264
TaaI	ACNGT	7 cut(s)	206, 805, 836, 1040, 1046, 1380, 1489
TaiI	ACGT	2 cut(s)	534, 905
TaqI	TCGA	2 cut(s)	1065, 1289
TasI	AATT	5 cut(s)	265, 480, 506, 1031, 1102
TatI	WGTACW	2 cut(s)	3, 446
TauI	GCSGC	2 cut(s)	194, 822
TfiI	GAWTC	4 cut(s)	167, 173, 475, 1519
Tru1I	TTAA	4 cut(s)	357, 654, 990, 1242
Tru9I	TTAA	4 cut(s)	357, 654, 990, 1242
TscAI	CASTG	7 cut(s)	247, 304, 817, 829, 975, 1159, 1383
TseFI	GTSAC	9 cut(s)	13, 391, 686, 808, 836, 1040, 1046, 1079, 1106
TseI	GCWGC	2 cut(s)	668, 984
Tsp45I	GTSAC	9 cut(s)	13, 391, 686, 808, 836, 1040, 1046, 1079, 1106
TspDTI	ATGAA	6 cut(s)	435, 779, 1060, 1412, 1439, 1489
TspGWI	ACGGA	1 cut(s)	116
TspRI	CASTG	7 cut(s)	247, 304, 817, 829, 975, 1159, 1383
Van91I	CCANNNNNTGG	2 cut(s)	664, 940
Vha464I	CTTAAG	1 cut(s)	356
VpaK11BI	GGWCC	3 cut(s)	124, 139, 920
XhoI	CTCGAG	1 cut(s)	1288
XmiI	GTMKAC	2 cut(s)	528, 732
XspI	CTAG	4 cut(s)	32, 366, 678, 1523
ZrmI	AGTACT	1 cut(s)	448

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.