FvH4_7g12901

B3 domain-containing protein At2g33720-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0007611
Physical Location & Seq
Reverse (-)
11665543 .. 11666461
919 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g12901.t1

Sequence Viewer

Length: 765 bp
ATGGAAGAGAAACCCTTGATGAAAACCCTAAATATGATCGACAAGTTAATGTATGAGGTGAATTCACCCTCATCTGGTAGTAGTAGTAGCAGTACCAATAAAACTGCAGAAAGCAGCCAACAACTTTGTGATGCACCCACTGATATAACTCTGCATCAATCTTGGTTGCAGCTGCCATCAAACACAAACAAGAGAAAAGCAAAAGCACCAGTAACACCGGAAAACGAAGATAGAATCCAGAAACCAGCCGCTCCTACTAATCCAGAGCATCAAAAACTAGTAGTCTCTGATCAAGAGAATATTGAGTCCAATTCAAAAAGTGATGATGGTGAAAACCTTCATGATCCAGAGCAGGTGGCGCGTGACCTGACCTTATACAAAATAAAGAAGAAGTTGACAATGAGTGACCTCGGAAACTTGAGCAGGCTCTTGGTGCCAGGTTATATGGTCAAGAACTTTGTTTTGCCTCTCGTGGATGCCGACTTTAAACAAAGAGTAGAGAGCAAAGCCGGTAAACAAGTTACGGTGAAAGACTCAGACAAAGAGACCGAGCATCAATTAACGTTCAGATACTTGAAGTCGTCGAAGAGTTATGTTTTCAATGGAAAATGGAAGGAGTTCGTGAAGAGGAGAGATCTGAAGAAGGACGATGAAATTGGACTTTACTGGGACACTCACGATTCCAAGTTCCACTTTTCTGTTCTGAAAGTTGCAAGCCAAGAAAGACAAGCAGCCACTGAAGTTGCTCGTGCTAATCAACAATGA

Protein Analysis

255

Amino Acids

28.92

Weight (kDa)

8.4

Isoelectric Point (pI)

46.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 130 - 234 3.9e-08 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 343
Acc36I ACCTGC 1 cut(s) 343
AccB1I GGYRCC 1 cut(s) 433
AccBSI CCGCTC 1 cut(s) 251
AccII CGCG 1 cut(s) 361
AciI CCGC 1 cut(s) 249
AclI AACGTT 1 cut(s) 563
AclWI GGATC 1 cut(s) 338
AcsI RAATTY 1 cut(s) 61
AcuI CTGAAG 2 cut(s) 659, 759
AfaI GTAC 1 cut(s) 94
AfiI CCNNNNNNNGG 1 cut(s) 74
AgsI TTSAA 3 cut(s) 315, 577, 601
AhlI ACTAGT 1 cut(s) 277
AjnI CCWGG 1 cut(s) 436
AluBI AGCT 1 cut(s) 172
AluI AGCT 1 cut(s) 172
Alw26I GTCTC 2 cut(s) 289, 539
AlwI GGATC 1 cut(s) 338
AlwNI CAGNNNCTG 1 cut(s) 737
ApeKI GCWGC 4 cut(s) 114, 169, 172, 731
ApoI RAATTY 1 cut(s) 61
Asp700I GAANNNNTTC 2 cut(s) 336, 617
AspLEI GCGC 1 cut(s) 361
AsuHPI GGTGA 4 cut(s) 57, 70, 341, 538
BanI GGYRCC 1 cut(s) 433
BauI CACGAG 2 cut(s) 470, 747
BbvI GCAGC 4 cut(s) 126, 159, 181, 743
BccI CCATC 2 cut(s) 184, 320
BciT130I CCWGG 1 cut(s) 438
BclI TGATCA 1 cut(s) 289
BcoDI GTCTC 2 cut(s) 289, 539
BcuI ACTAGT 1 cut(s) 277
BfaI CTAG 1 cut(s) 278
BfmI CTRYAG 1 cut(s) 105
BfuAI ACCTGC 1 cut(s) 343
BglII AGATCT 1 cut(s) 634
BisI GCNGC 5 cut(s) 115, 170, 173, 249, 732
BlsI GCNGC 5 cut(s) 116, 171, 174, 250, 733
Bme1390I CCNGG 1 cut(s) 438
BmiI GGNNCC 1 cut(s) 435
BmrFI CCNGG 1 cut(s) 438
BmrI ACTGGG 1 cut(s) 676
BmsI GCATC 5 cut(s) 121, 163, 277, 466, 562
BmuI ACTGGG 1 cut(s) 676
BpuEI CTTGAG 1 cut(s) 439
BsaI GGTCTC 1 cut(s) 539
BsaJI CCNNGG 1 cut(s) 409
BsaWI WCCGGW 1 cut(s) 217
Bsc4I CCNNNNNNNGG 1 cut(s) 74
Bse118I RCCGGY 1 cut(s) 509
Bse1I ACTGG 2 cut(s) 209, 671
BseBI CCWGG 1 cut(s) 438
BseDI CCNNGG 1 cut(s) 409
BseGI GGATG 1 cut(s) 481
BseLI CCNNNNNNNGG 1 cut(s) 74
BseMII CTCAG 1 cut(s) 549
BseNI ACTGG 2 cut(s) 209, 671
BseRI GAGGAG 1 cut(s) 643
BseXI GCAGC 4 cut(s) 126, 159, 181, 743
Bsh1236I CGCG 1 cut(s) 361
BshNI GGYRCC 1 cut(s) 433
BsiSI CCGG 2 cut(s) 218, 510
BslFI GGGAC 1 cut(s) 683
BslI CCNNNNNNNGG 1 cut(s) 74
BsmAI GTCTC 2 cut(s) 289, 539
BsmFI GGGAC 1 cut(s) 683
Bso31I GGTCTC 1 cut(s) 539
Bsp143I GATC 4 cut(s) 36, 289, 343, 634
BspACI CCGC 1 cut(s) 249
BspCNI CTCAG 1 cut(s) 548
BspFNI CGCG 1 cut(s) 361
BspHI TCATGA 1 cut(s) 340
BspLI GGNNCC 1 cut(s) 435
BspMAI CTGCAG 1 cut(s) 109
BspMI ACCTGC 1 cut(s) 343
BspPI GGATC 1 cut(s) 338
BspT107I GGYRCC 1 cut(s) 433
BspTNI GGTCTC 1 cut(s) 539
BsrBI CCGCTC 1 cut(s) 251
BsrFI RCCGGY 1 cut(s) 509
BsrI ACTGG 2 cut(s) 209, 671
BssAI RCCGGY 1 cut(s) 509
BssECI CCNNGG 1 cut(s) 409
BssMI GATC 4 cut(s) 36, 289, 343, 634
BssSI CACGAG 2 cut(s) 470, 747
Bst2BI CACGAG 2 cut(s) 470, 747
Bst2UI CCWGG 1 cut(s) 438
Bst4CI ACNGT 1 cut(s) 526
Bst6I CTCTTC 2 cut(s) 581, 620
BstC8I GCNNGC 2 cut(s) 425, 715
BstDEI CTNAG 1 cut(s) 535
BstF5I GGATG 1 cut(s) 481
BstFNI CGCG 1 cut(s) 361
BstHHI GCGC 1 cut(s) 361
BstKTI GATC 4 cut(s) 39, 292, 346, 637
BstMAI GTCTC 2 cut(s) 289, 539
BstMBI GATC 4 cut(s) 36, 289, 343, 634
BstMWI GCNNNNNNNGC 2 cut(s) 358, 433
BstNI CCWGG 1 cut(s) 438
BstSCI CCNGG 1 cut(s) 436
BstSFI CTRYAG 1 cut(s) 105
BstUI CGCG 1 cut(s) 361
BstV1I GCAGC 4 cut(s) 126, 159, 181, 743
BstX2I RGATCY 1 cut(s) 634
BstYI RGATCY 1 cut(s) 634
BtsCI GGATG 1 cut(s) 481
BtsIMutI CAGTG 2 cut(s) 138, 735
BveI ACCTGC 1 cut(s) 343
Cac8I GCNNGC 2 cut(s) 425, 715
CaiI CAGNNNCTG 1 cut(s) 737
CciI TCATGA 1 cut(s) 340
CfoI GCGC 1 cut(s) 361
Cfr10I RCCGGY 1 cut(s) 509
Csp6I GTAC 1 cut(s) 93
CspCI CAANNNNNGTGG 2 cut(s) 724, 759
CviAII CATG 1 cut(s) 341
CviJI RGCY 7 cut(s) 117, 172, 248, 427, 509, 717, 734
CviKI_1 RGCY 7 cut(s) 117, 172, 248, 427, 509, 717, 734
CviQI GTAC 1 cut(s) 93
DdeI CTNAG 1 cut(s) 535
DpnI GATC 4 cut(s) 38, 291, 345, 636
DpnII GATC 4 cut(s) 36, 289, 343, 634
DraI TTTAAA 1 cut(s) 487
Eam1104I CTCTTC 2 cut(s) 581, 620
EarI CTCTTC 2 cut(s) 581, 620
Eco31I GGTCTC 1 cut(s) 539
Eco57I CTGAAG 2 cut(s) 659, 759
EcoRI GAATTC 1 cut(s) 61
EcoRII CCWGG 1 cut(s) 436
FaeI CATG 1 cut(s) 344
FaiI YATR 8 cut(s) 35, 54, 146, 342, 376, 444, 446, 594
FalI AAGNNNNNCTT 2 cut(s) 677, 709
FaqI GGGAC 1 cut(s) 683
FatI CATG 1 cut(s) 340
FbaI TGATCA 1 cut(s) 289
Fnu4HI GCNGC 5 cut(s) 115, 170, 173, 249, 732
FokI GGATG 1 cut(s) 488
Fsp4HI GCNGC 5 cut(s) 115, 170, 173, 249, 732
FspBI CTAG 1 cut(s) 278
GlaI GCGC 1 cut(s) 360
GluI GCNGC 5 cut(s) 115, 170, 173, 249, 732
HapII CCGG 2 cut(s) 218, 510
HhaI GCGC 1 cut(s) 361
Hin1II CATG 1 cut(s) 344
Hin6I GCGC 1 cut(s) 359
HinP1I GCGC 1 cut(s) 359
HincII GTYRAC 1 cut(s) 396
HindII GTYRAC 1 cut(s) 396
HinfI GANTC 4 cut(s) 234, 305, 533, 680
HpaII CCGG 2 cut(s) 218, 510
HphI GGTGA 4 cut(s) 57, 70, 341, 538
Hpy166II GTNNAC 2 cut(s) 396, 515
Hpy188I TCNGA 6 cut(s) 289, 413, 538, 569, 639, 705
Hpy188III TCNNGA 8 cut(s) 238, 263, 293, 341, 347, 451, 622, 677
Hpy8I GTNNAC 2 cut(s) 396, 515
Hpy99I CGWCG 1 cut(s) 586
HpyAV CCTTC 3 cut(s) 347, 607, 637
HpyCH4III ACNGT 1 cut(s) 526
HpyCH4IV ACGT 1 cut(s) 563
HpyCH4V TGCA 5 cut(s) 107, 134, 154, 169, 713
HpyF10VI GCNNNNNNNGC 2 cut(s) 358, 433
HpyF3I CTNAG 1 cut(s) 535
HpySE526I ACGT 1 cut(s) 563
Hsp92II CATG 1 cut(s) 344
HspAI GCGC 1 cut(s) 359
Ksp22I TGATCA 1 cut(s) 289
Kzo9I GATC 4 cut(s) 36, 289, 343, 634
LmnI GCTCC 1 cut(s) 256
Lsp1109I GCAGC 4 cut(s) 126, 159, 181, 743
LweI GCATC 5 cut(s) 121, 163, 277, 466, 562
MaeI CTAG 1 cut(s) 278
MaeII ACGT 1 cut(s) 563
MaeIII GTNAC 4 cut(s) 211, 362, 404, 520
MalI GATC 4 cut(s) 38, 291, 345, 636
MbiI CCGCTC 1 cut(s) 251
MboI GATC 4 cut(s) 36, 289, 343, 634
MboII GAAGA 6 cut(s) 17, 239, 400, 598, 637, 652
MflI RGATCY 1 cut(s) 634
MluCI AATT 4 cut(s) 61, 310, 557, 654
MlyI GAGTC 2 cut(s) 314, 527
MnlI CCTC 5 cut(s) 49, 79, 419, 477, 621
MroXI GAANNNNTTC 2 cut(s) 336, 617
MseI TTAA 3 cut(s) 47, 486, 560
MspA1I CMGCKG 1 cut(s) 172
MspI CCGG 2 cut(s) 218, 510
MspR9I CCNGG 1 cut(s) 438
MvaI CCWGG 1 cut(s) 438
MvnI CGCG 1 cut(s) 361
MwoI GCNNNNNNNGC 2 cut(s) 358, 433
NdeII GATC 4 cut(s) 36, 289, 343, 634
NlaIII CATG 1 cut(s) 344
NlaIV GGNNCC 1 cut(s) 435
NmuCI GTSAC 2 cut(s) 362, 404
PagI TCATGA 1 cut(s) 340
PaqCI CACCTGC 1 cut(s) 343
PcsI WCGNNNNNNNCGW 1 cut(s) 477
PdmI GAANNNNTTC 2 cut(s) 336, 617
PfeI GAWTC 2 cut(s) 234, 680
PkrI GCNGC 5 cut(s) 116, 171, 174, 250, 733
PleI GAGTC 2 cut(s) 313, 527
PpsI GAGTC 2 cut(s) 313, 527
Psp1406I AACGTT 1 cut(s) 563
Psp6I CCWGG 1 cut(s) 436
PspGI CCWGG 1 cut(s) 436
PspN4I GGNNCC 1 cut(s) 435
PstI CTGCAG 1 cut(s) 109
PstNI CAGNNNCTG 1 cut(s) 737
PsuI RGATCY 1 cut(s) 634
PvuII CAGCTG 1 cut(s) 172
RsaI GTAC 1 cut(s) 94
RsaNI GTAC 1 cut(s) 93
SaqAI TTAA 3 cut(s) 47, 486, 560
SatI GCNGC 5 cut(s) 115, 170, 173, 249, 732
Sau3AI GATC 4 cut(s) 36, 289, 343, 634
SchI GAGTC 2 cut(s) 314, 527
ScrFI CCNGG 1 cut(s) 438
SetI ASST 9 cut(s) 60, 174, 339, 357, 369, 374, 411, 442, 566
SfaNI GCATC 5 cut(s) 121, 163, 277, 466, 562
SfcI CTRYAG 1 cut(s) 105
SmlI CTYRAG 1 cut(s) 418
SmoI CTYRAG 1 cut(s) 418
SpeI ACTAGT 1 cut(s) 277
Sse9I AATT 4 cut(s) 61, 310, 557, 654
SsiI CCGC 1 cut(s) 249
SspI AATATT 1 cut(s) 301
SspMI CTAG 1 cut(s) 278
StyD4I CCNGG 1 cut(s) 436
TaaI ACNGT 1 cut(s) 526
TaiI ACGT 1 cut(s) 566
TaqI TCGA 2 cut(s) 39, 584
TaqII GACCGA 1 cut(s) 563
TasI AATT 4 cut(s) 61, 310, 557, 654
TauI GCSGC 1 cut(s) 251
TfiI GAWTC 2 cut(s) 234, 680
Tru1I TTAA 3 cut(s) 47, 486, 560
Tru9I TTAA 3 cut(s) 47, 486, 560
TscAI CASTG 2 cut(s) 145, 742
TseFI GTSAC 2 cut(s) 362, 404
TseI GCWGC 4 cut(s) 114, 169, 172, 731
Tsp45I GTSAC 2 cut(s) 362, 404
TspDTI ATGAA 3 cut(s) 35, 329, 666
TspRI CASTG 2 cut(s) 145, 742
XapI RAATTY 1 cut(s) 61
XmnI GAANNNNTTC 2 cut(s) 336, 617
XspI CTAG 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.