Rh1DG234400

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0007611
Physical Location & Seq
Reverse (-)
44709699 .. 44711043
1345 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG234400.1

Sequence Viewer

Length: 816 bp
ATGGAAGAGAAACCCTCAAAAACCCTCAATTTGATCGACAAGTTAATGCATGCAGCAGAATCACCCTCAACTGCTAGTAGTAGCAGAACCAGTACTGCAGGAGACAACCAACAACTCTGTGATGCAGCCACTGCTTTAACTTTGCATCAATCTTGGTTGCAGCCGGCATCGACCACAAACAAGAGAAAAGCAAAAGCACCAGTTATACCGGAAAAGGAAGACAGAATCGAGAAACCAGCCGCTCCTACTAATCCGAAACATCAAAAACTAGTTCTCTCTGATCATGAGAATATAGAGTTGAAGAAGAAACGTGACCCAGTAGTGGAATGTGATGATGCTCAGAACCTTCATACACTTGGTGAAGGGAGCGCTAGGCCGAGTGCTCGTCATCATATTGATGAGCAGGTGGCTCGTGACCCAGCCTTATACAAAATAAAGAAGAAGTTGACTCTAAGTGACCTTGGAAACTTGAGCAGGCTCTTGGTGCCGAAAGAGATGGTCAAGAAACATGTTCTGCCTTCGGTGGATGCCGACTTTGTACAAAGACTCGAGAGCAAAGCCGGTAAACAAGTTACGGTTAAAGACTCCGACAAAGAGACCGAGCATCAATTAACTTTTAGATACTTGAGGTCGTCGAAGAGTTATGTTTTCAATGGAAATTGGATGGAAATAGTGAAGGGAAGAGATTTGAAGAAGGATGATGAAATTGGACTTTACTGGGACACTCTCAATTCCAAGTTCCACTTTTCTCTTCTGAAAGTTGCAAGCCAAGAAAGCCAAGCTGCCGATGAAGTTACTCGTGTTCATCAACAATGA

Protein Analysis

271

Amino Acids

30.52

Weight (kDa)

8.84

Isoelectric Point (pI)

47.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 147 - 250 1.5e-07 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 394
Acc36I ACCTGC 1 cut(s) 394
AccB1I GGYRCC 1 cut(s) 484
AccBSI CCGCTC 1 cut(s) 242
AciI CCGC 1 cut(s) 240
AdeI CACNNNGTG 1 cut(s) 359
AfaI GTAC 2 cut(s) 94, 540
AfeI AGCGCT 1 cut(s) 370
AfiI CCNNNNNNNGG 1 cut(s) 322
AflIII ACRYGT 1 cut(s) 508
AgsI TTSAA 3 cut(s) 301, 652, 691
AhlI ACTAGT 1 cut(s) 268
AluBI AGCT 1 cut(s) 782
AluI AGCT 1 cut(s) 782
Alw21I GWGCWC 1 cut(s) 385
Alw26I GTCTC 2 cut(s) 96, 590
AlwNI CAGNNNCTG 1 cut(s) 131
Ama87I CYCGRG 1 cut(s) 548
Aor51HI AGCGCT 1 cut(s) 370
AoxI GGCC 1 cut(s) 374
ApeKI GCWGC 4 cut(s) 53, 125, 160, 782
AspLEI GCGC 1 cut(s) 371
AsuHPI GGTGA 2 cut(s) 54, 371
AvaI CYCGRG 1 cut(s) 548
BanI GGYRCC 1 cut(s) 484
BauI CACGAG 2 cut(s) 411, 798
BbsI GAAGAC 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 385
BbvI GCAGC 4 cut(s) 65, 137, 172, 769
BccI CCATC 2 cut(s) 490, 658
BcgI CGANNNNNNTGC 2 cut(s) 392, 426
BclI TGATCA 1 cut(s) 280
BcoDI GTCTC 2 cut(s) 96, 590
BcuI ACTAGT 1 cut(s) 268
BfaI CTAG 3 cut(s) 75, 269, 372
BfmI CTRYAG 1 cut(s) 96
BfoI RGCGCY 1 cut(s) 372
BfuAI ACCTGC 1 cut(s) 394
BisI GCNGC 5 cut(s) 54, 126, 161, 240, 783
BlsI GCNGC 5 cut(s) 55, 127, 162, 241, 784
BmcAI AGTACT 1 cut(s) 94
BmeT110I CYCGRG 1 cut(s) 548
BmiI GGNNCC 1 cut(s) 486
BmrI ACTGGG 2 cut(s) 311, 727
BmsI GCATC 6 cut(s) 112, 154, 176, 325, 517, 613
BmuI ACTGGG 2 cut(s) 311, 727
BpiI GAAGAC 1 cut(s) 225
BplI GAGNNNNNCTC 1 cut(s) 31
BpuEI CTTGAG 2 cut(s) 490, 646
BsaI GGTCTC 1 cut(s) 590
BsaJI CCNNGG 1 cut(s) 460
BsaWI WCCGGW 1 cut(s) 208
Bsc4I CCNNNNNNNGG 1 cut(s) 322
Bse118I RCCGGY 2 cut(s) 163, 560
Bse1I ACTGG 4 cut(s) 90, 200, 317, 722
BseDI CCNNGG 1 cut(s) 460
BseGI GGATG 3 cut(s) 532, 669, 703
BseLI CCNNNNNNNGG 1 cut(s) 322
BseMII CTCAG 1 cut(s) 353
BseNI ACTGG 4 cut(s) 90, 200, 317, 722
BseXI GCAGC 4 cut(s) 65, 137, 172, 769
BseYI CCCAGC 1 cut(s) 418
BshFI GGCC 1 cut(s) 376
BshNI GGYRCC 1 cut(s) 484
BsiHKAI GWGCWC 1 cut(s) 385
BsiHKCI CYCGRG 1 cut(s) 548
BsiSI CCGG 3 cut(s) 164, 209, 561
BslFI GGGAC 1 cut(s) 734
BslI CCNNNNNNNGG 1 cut(s) 322
BsmAI GTCTC 2 cut(s) 96, 590
BsmFI GGGAC 1 cut(s) 734
BsnI GGCC 1 cut(s) 376
Bso31I GGTCTC 1 cut(s) 590
BsoBI CYCGRG 1 cut(s) 548
Bsp1286I GDGCHC 1 cut(s) 385
Bsp1407I TGTACA 1 cut(s) 538
Bsp143I GATC 2 cut(s) 33, 280
BspACI CCGC 1 cut(s) 240
BspANI GGCC 1 cut(s) 376
BspCNI CTCAG 1 cut(s) 352
BspHI TCATGA 1 cut(s) 283
BspLI GGNNCC 1 cut(s) 486
BspMAI CTGCAG 1 cut(s) 100
BspMI ACCTGC 1 cut(s) 394
BspT107I GGYRCC 1 cut(s) 484
BspTNI GGTCTC 1 cut(s) 590
BsrBI CCGCTC 1 cut(s) 242
BsrFI RCCGGY 2 cut(s) 163, 560
BsrGI TGTACA 1 cut(s) 538
BsrI ACTGG 4 cut(s) 90, 200, 317, 722
BssAI RCCGGY 2 cut(s) 163, 560
BssECI CCNNGG 1 cut(s) 460
BssMI GATC 2 cut(s) 33, 280
BssSI CACGAG 2 cut(s) 411, 798
BssT1I CCWWGG 1 cut(s) 460
Bst2BI CACGAG 2 cut(s) 411, 798
Bst4CI ACNGT 1 cut(s) 577
Bst6I CTCTTC 3 cut(s) 632, 676, 756
BstAPI GCANNNNNTGC 1 cut(s) 131
BstAUI TGTACA 1 cut(s) 538
BstC8I GCNNGC 4 cut(s) 51, 165, 476, 766
BstDEI CTNAG 2 cut(s) 339, 452
BstF5I GGATG 3 cut(s) 532, 669, 703
BstH2I RGCGCY 1 cut(s) 372
BstHHI GCGC 1 cut(s) 371
BstKTI GATC 2 cut(s) 36, 283
BstMAI GTCTC 2 cut(s) 96, 590
BstMBI GATC 2 cut(s) 33, 280
BstMWI GCNNNNNNNGC 3 cut(s) 131, 484, 774
BstNSI RCATGY 2 cut(s) 53, 512
BstSFI CTRYAG 1 cut(s) 96
BstV1I GCAGC 4 cut(s) 65, 137, 172, 769
BstV2I GAAGAC 1 cut(s) 225
BsuRI GGCC 1 cut(s) 376
BtsCI GGATG 3 cut(s) 532, 669, 703
BtsI GCAGTG 1 cut(s) 129
BtsIMutI CAGTG 1 cut(s) 129
BveI ACCTGC 1 cut(s) 394
Cac8I GCNNGC 4 cut(s) 51, 165, 476, 766
CaiI CAGNNNCTG 1 cut(s) 131
CciI TCATGA 1 cut(s) 283
CfoI GCGC 1 cut(s) 371
Cfr10I RCCGGY 2 cut(s) 163, 560
Csp6I GTAC 2 cut(s) 93, 539
CviAII CATG 3 cut(s) 50, 284, 509
CviQI GTAC 2 cut(s) 93, 539
DdeI CTNAG 2 cut(s) 339, 452
DpnI GATC 2 cut(s) 35, 282
DpnII GATC 2 cut(s) 33, 280
DraIII CACNNNGTG 1 cut(s) 359
Eam1104I CTCTTC 3 cut(s) 632, 676, 756
EarI CTCTTC 3 cut(s) 632, 676, 756
Eco130I CCWWGG 1 cut(s) 460
Eco31I GGTCTC 1 cut(s) 590
Eco47III AGCGCT 1 cut(s) 370
Eco88I CYCGRG 1 cut(s) 548
EcoT14I CCWWGG 1 cut(s) 460
EcoT22I ATGCAT 1 cut(s) 51
ErhI CCWWGG 1 cut(s) 460
FaeI CATG 3 cut(s) 53, 287, 512
FaiI YATR 9 cut(s) 51, 206, 285, 293, 351, 393, 427, 510, 645
FalI AAGNNNNNCTT 2 cut(s) 728, 760
FaqI GGGAC 1 cut(s) 734
FatI CATG 3 cut(s) 49, 283, 508
FbaI TGATCA 1 cut(s) 280
Fnu4HI GCNGC 5 cut(s) 54, 126, 161, 240, 783
FokI GGATG 3 cut(s) 539, 676, 710
Fsp4HI GCNGC 5 cut(s) 54, 126, 161, 240, 783
FspBI CTAG 3 cut(s) 75, 269, 372
GlaI GCGC 1 cut(s) 370
GluI GCNGC 5 cut(s) 54, 126, 161, 240, 783
GsaI CCCAGC 1 cut(s) 422
HaeII RGCGCY 1 cut(s) 372
HaeIII GGCC 1 cut(s) 376
HapII CCGG 3 cut(s) 164, 209, 561
HhaI GCGC 1 cut(s) 371
Hin1II CATG 3 cut(s) 53, 287, 512
Hin6I GCGC 1 cut(s) 369
HinP1I GCGC 1 cut(s) 369
HincII GTYRAC 1 cut(s) 447
HindII GTYRAC 1 cut(s) 447
HinfI GANTC 5 cut(s) 59, 225, 448, 546, 584
HpaII CCGG 3 cut(s) 164, 209, 561
HphI GGTGA 2 cut(s) 54, 371
Hpy166II GTNNAC 2 cut(s) 447, 566
Hpy188I TCNGA 5 cut(s) 255, 280, 342, 589, 756
Hpy188III TCNNGA 5 cut(s) 229, 284, 413, 502, 550
Hpy8I GTNNAC 2 cut(s) 447, 566
Hpy99I CGWCG 1 cut(s) 637
HpyAV CCTTC 5 cut(s) 356, 356, 528, 670, 688
HpyCH4III ACNGT 1 cut(s) 577
HpyCH4IV ACGT 1 cut(s) 310
HpyCH4V TGCA 7 cut(s) 49, 53, 98, 125, 145, 160, 764
HpyF10VI GCNNNNNNNGC 3 cut(s) 131, 484, 774
HpyF3I CTNAG 2 cut(s) 339, 452
HpySE526I ACGT 1 cut(s) 310
Hsp92II CATG 3 cut(s) 53, 287, 512
HspAI GCGC 1 cut(s) 369
KroI GCCGGC 1 cut(s) 163
KroNI GCCGGC 1 cut(s) 165
Ksp22I TGATCA 1 cut(s) 280
Kzo9I GATC 2 cut(s) 33, 280
LmnI GCTCC 2 cut(s) 247, 366
Lsp1109I GCAGC 4 cut(s) 65, 137, 172, 769
LweI GCATC 6 cut(s) 112, 154, 176, 325, 517, 613
MaeI CTAG 3 cut(s) 75, 269, 372
MaeII ACGT 1 cut(s) 310
MaeIII GTNAC 5 cut(s) 311, 413, 455, 571, 793
MalI GATC 2 cut(s) 35, 282
MbiI CCGCTC 1 cut(s) 242
MboI GATC 2 cut(s) 33, 280
MboII GAAGA 9 cut(s) 17, 230, 313, 316, 451, 649, 693, 703, 743
MhlI GDGCHC 1 cut(s) 385
MluCI AATT 5 cut(s) 28, 608, 658, 705, 730
MlyI GAGTC 3 cut(s) 442, 540, 578
MmeI TCCRAC 1 cut(s) 612
MnlI CCTC 4 cut(s) 25, 35, 76, 621
Mph1103I ATGCAT 1 cut(s) 51
MroNI GCCGGC 1 cut(s) 163
MseI TTAA 4 cut(s) 44, 137, 579, 611
MslI CAYNNNNRTG 1 cut(s) 396
MspI CCGG 3 cut(s) 164, 209, 561
MwoI GCNNNNNNNGC 3 cut(s) 131, 484, 774
NaeI GCCGGC 1 cut(s) 165
NdeII GATC 2 cut(s) 33, 280
NgoMIV GCCGGC 1 cut(s) 163
NlaIII CATG 3 cut(s) 53, 287, 512
NlaIV GGNNCC 1 cut(s) 486
NmeAIII GCCGAG 1 cut(s) 402
NmuCI GTSAC 3 cut(s) 311, 413, 455
NsiI ATGCAT 1 cut(s) 51
NspI RCATGY 2 cut(s) 53, 512
PaeI GCATGC 1 cut(s) 53
PaeR7I CTCGAG 1 cut(s) 548
PagI TCATGA 1 cut(s) 283
PaqCI CACCTGC 1 cut(s) 394
PciI ACATGT 1 cut(s) 508
PdiI GCCGGC 1 cut(s) 165
PfeI GAWTC 2 cut(s) 59, 225
PkrI GCNGC 5 cut(s) 55, 127, 162, 241, 784
PleI GAGTC 3 cut(s) 442, 540, 578
PpsI GAGTC 3 cut(s) 442, 540, 578
PscI ACATGT 1 cut(s) 508
PspFI CCCAGC 1 cut(s) 418
PspN4I GGNNCC 1 cut(s) 486
PstI CTGCAG 1 cut(s) 100
PstNI CAGNNNCTG 1 cut(s) 131
RsaI GTAC 2 cut(s) 94, 540
RsaNI GTAC 2 cut(s) 93, 539
RseI CAYNNNNRTG 1 cut(s) 396
SaqAI TTAA 4 cut(s) 44, 137, 579, 611
SatI GCNGC 5 cut(s) 54, 126, 161, 240, 783
Sau3AI GATC 2 cut(s) 33, 280
ScaI AGTACT 1 cut(s) 94
SchI GAGTC 3 cut(s) 442, 540, 578
SduI GDGCHC 1 cut(s) 385
SetI ASST 6 cut(s) 313, 348, 408, 462, 632, 784
SfaNI GCATC 6 cut(s) 112, 154, 176, 325, 517, 613
SfcI CTRYAG 1 cut(s) 96
Sfr274I CTCGAG 1 cut(s) 548
SlaI CTCGAG 1 cut(s) 548
SmiMI CAYNNNNRTG 1 cut(s) 396
SmlI CTYRAG 3 cut(s) 469, 548, 625
SmoI CTYRAG 3 cut(s) 469, 548, 625
SpeI ACTAGT 1 cut(s) 268
SphI GCATGC 1 cut(s) 53
Sse9I AATT 5 cut(s) 28, 608, 658, 705, 730
SsiI CCGC 1 cut(s) 240
SspMI CTAG 3 cut(s) 75, 269, 372
StyI CCWWGG 1 cut(s) 460
TaaI ACNGT 1 cut(s) 577
TaiI ACGT 1 cut(s) 313
TaqI TCGA 5 cut(s) 36, 170, 228, 549, 635
TaqII GACCGA 1 cut(s) 614
TasI AATT 5 cut(s) 28, 608, 658, 705, 730
TatI WGTACW 2 cut(s) 92, 538
TauI GCSGC 1 cut(s) 242
TfiI GAWTC 2 cut(s) 59, 225
Tru1I TTAA 4 cut(s) 44, 137, 579, 611
Tru9I TTAA 4 cut(s) 44, 137, 579, 611
TscAI CASTG 1 cut(s) 136
TseFI GTSAC 3 cut(s) 311, 413, 455
TseI GCWGC 4 cut(s) 53, 125, 160, 782
Tsp45I GTSAC 3 cut(s) 311, 413, 455
TspDTI ATGAA 4 cut(s) 338, 717, 794, 804
TspRI CASTG 1 cut(s) 136
XceI RCATGY 2 cut(s) 53, 512
XhoI CTCGAG 1 cut(s) 548
XspI CTAG 3 cut(s) 75, 269, 372
ZrmI AGTACT 1 cut(s) 94
Zsp2I ATGCAT 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.