Rorug01G0223200

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0007611
Physical Location & Seq
Forward (+)
32097620 .. 32099851
2232 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0223200.1

Sequence Viewer

Length: 1821 bp
ATGGAGGATTCAGCCCCTTTCAGAAAACCCCATTTCCCAAATCCAGGGATGAACAACTCATCAGCAGTTCAGTTTCAGCAGAACCCCATTACACAAGGTAAGTCTTACCTGCAATTTCTGTTTAAAGGCATCTTCTTTGTGGTTTTGGTCGTGGCTCTGCCTCTGTTTCCTTCACAAGCTCCTGAATTTATCAACGATACGATACTCGACAAGTTCTGGGAGCTTATTCATCTTCTTTTCATTGGAATTGCTGTGTCTTATGGCTTGTTTAGCAGAAGAAATGTTGAGATGGGGATTGAAAACCAGTCAAATGTTGAAAATGTAGAGTCTTATATGCCTAGGATGTTTGATGGGTATGAAAATCCAGGTGGGTCTGGTGAGAAGAAAGTTCTTGAGAGTTGGAGTTCTCAGAATTTTGTAGGTAACCATAAAAGTACTGTTTATTTTGATGCCCAATGCAAACCCAGATTGCCAAATTGTGGAGATGGTCTTGAAAATTCTTGTGGTTTTAAAAGTAAGAATGTTGCTCAATCTTGGAATTCTCAGTACTTTCAGGGTGAATCTATGGTTTTTGTAGCTCAACCAGATTATGCTGTTGATGAGTTTGTGAAACCCAAATCAGTAGTTGATGATGAGCCATTAGGATTGCCTGTTCGGAGTTTGAAATCGAGGGTTAAAAACCAGGAGAAGCCTGAGTTTGTTACTAGGAGTGAGTCTGGTTTTGGTTCAAAGGTTTCATCTAATAGTTTTGACTGGAGTAGGAGTAAGAACTTTGGTGATTTGGCTCCTATTAATTTACTAGAGAAATTCAATCAAGCTATGGCATCACCTTCCCCAGTTCTTCAGCGTTCGAGTTCTCGAAAGATGGAAAGGGGAAAAAGAGTAGGTAGTGCCCCTCGTCCTTCACATTTTAGGCCACTGTCAGTTGATGAAGCTCAATTTGAGTCGCTAAAAACTCGGTCCTTCCGGTCCACATTGTCTTTCTCATCCGAAACTAGCTCCAAATCTTCTTCACCTGAAAAGGAGTACTCTTCTGTTAACTCTACAACTTCTGAGGTACCAAACCCGGAAACTGTTCATTTGGGGAAAAGAAAGAAGAGCTCCCGAGCGTCTTCTCCTTCAGGTTTACCATCAGAACCAAATCCAATGATTGAAAAAGTTTCAATGGGTGGATTGCATTCGAGGGGCTATAGTGTAGGTTCTTTCTTTGAGGAGGATTTGAGGAAAAGCTCAGAGAATCACTTGAAGAAAGAATTGAATGGGAGTCGAATTACTGGGGATCAATATGATTCAGGTAAGAAAGAGTTGGGGACAGGGTCTTTCGGATCAGAGAAGAAGCCTGCAAGCCTCAGCAAATCTACATCAAGAGGAAAATCAGTCAGAACAATCAGGGGTAGTAGACTGACCACTCATGACAAGGTTGAAAACAATCAAAAGATGTTTGATAATGGTGAAGCTGTATTTATGAGGAAAGAGAAAATGCAAAATGGAGGACTTGATAATCTGTGTGATATGCCAAAGCCAGCTTTTCCCAAGTACCAGAAGGAACAGTTACAAGACTTTTTTAACCATCTCAATGTGCAGTCTGAAGAAGAATCGGAAAGTGAGGCTGAAAACTTTGAAGTGAGCTCAGAAGATAGAGTGGAAGCTGCTGCTGCTGCATTTTGCAACTCTGTCAATGTTGCAGCAGGACCAGACTCTGAGGTTGATAAGAAGGCTGGTGAGTTTATAGCCAAATTTAGAGAGCAGATAAGGCGTCAGAAAGTTGCATCAATCGACAAATCAAGAGGGCTCGGCGTTAGTGGGAATCGCTCTAGGTGA

Protein Analysis

606

Amino Acids

67.45

Weight (kDa)

8.85

Isoelectric Point (pI)

51.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF761 PF05553 567 - 595 3e-09 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 117
Acc65I GGTACC 1 cut(s) 1057
AccB1I GGYRCC 1 cut(s) 1057
AccB7I CCANNNNNTGG 1 cut(s) 479
AccI GTMKAC 1 cut(s) 1399
AclWI GGATC 2 cut(s) 1287, 1333
AcsI RAATTY 6 cut(s) 185, 412, 496, 538, 806, 1736
AcuI CTGAAG 3 cut(s) 827, 1104, 1608
AcyI GRCGYC 1 cut(s) 1756
AfaI GTAC 5 cut(s) 436, 548, 1028, 1059, 1538
AfiI CCNNNNNNNGG 2 cut(s) 44, 479
AjnI CCWGG 3 cut(s) 43, 364, 681
AloI GAACNNNNNNTCC 2 cut(s) 636, 668
Alw21I GWGCWC 2 cut(s) 1103, 1631
AlwI GGATC 2 cut(s) 1287, 1333
AlwNI CAGNNNCTG 1 cut(s) 1700
Ama87I CYCGRG 1 cut(s) 1104
AoxI GGCC 1 cut(s) 914
ApeKI GCWGC 5 cut(s) 1649, 1652, 1655, 1658, 1685
ApoI RAATTY 6 cut(s) 185, 412, 496, 538, 806, 1736
AseI ATTAAT 1 cut(s) 792
Asp700I GAANNNNTTC 1 cut(s) 1074
Asp718I GGTACC 1 cut(s) 1057
AspA2I CCTAGG 1 cut(s) 338
AspS9I GGNCC 3 cut(s) 960, 969, 1691
AsuC2I CCSGG 1 cut(s) 1067
AsuHPI GGTGA 7 cut(s) 389, 569, 788, 819, 1005, 1463, 1733
AvaI CYCGRG 1 cut(s) 1104
AvaII GGWCC 3 cut(s) 960, 969, 1691
AvrII CCTAGG 1 cut(s) 338
BaeGI GKGCMC 1 cut(s) 895
BanI GGYRCC 1 cut(s) 1057
BanII GRGCYC 3 cut(s) 1103, 1631, 1794
BbsI GAAGAC 1 cut(s) 1104
Bbv12I GWGCWC 2 cut(s) 1103, 1631
BbvCI CCTCAGC 1 cut(s) 1349
BbvI GCAGC 5 cut(s) 1636, 1639, 1642, 1645, 1697
BccI CCATC 6 cut(s) 283, 344, 479, 859, 1138, 1578
BciT130I CCWGG 3 cut(s) 45, 366, 683
BcnI CCSGG 1 cut(s) 1067
BfaI CTAG 5 cut(s) 339, 705, 800, 996, 1815
BfmI CTRYAG 1 cut(s) 1189
BfuAI ACCTGC 1 cut(s) 117
BisI GCNGC 5 cut(s) 1650, 1653, 1656, 1659, 1686
BlnI CCTAGG 1 cut(s) 338
BlsI GCNGC 5 cut(s) 1651, 1654, 1657, 1660, 1687
BmcAI AGTACT 3 cut(s) 436, 548, 1028
Bme1390I CCNGG 4 cut(s) 45, 366, 683, 1067
Bme18I GGWCC 3 cut(s) 960, 969, 1691
BmeT110I CYCGRG 1 cut(s) 1104
BmgT120I GGNCC 3 cut(s) 960, 969, 1691
BmiI GGNNCC 2 cut(s) 786, 1059
BmrFI CCNGG 4 cut(s) 45, 366, 683, 1067
BmrI ACTGGG 2 cut(s) 830, 1284
BmsI GCATC 4 cut(s) 138, 439, 833, 1778
BmuI ACTGGG 2 cut(s) 830, 1284
BpiI GAAGAC 1 cut(s) 1104
BpmI CTGGAG 1 cut(s) 775
Bpu10I CCTNAGC 1 cut(s) 1349
BpuEI CTTGAG 1 cut(s) 413
BpuMI CCSGG 1 cut(s) 1067
BsaHI GRCGYC 1 cut(s) 1756
BsaJI CCNNGG 2 cut(s) 44, 338
BsaWI WCCGGW 1 cut(s) 966
BsaXI ACNNNNNCTCC 2 cut(s) 748, 778
Bsc4I CCNNNNNNNGG 2 cut(s) 44, 479
Bse1I ACTGG 4 cut(s) 304, 758, 836, 1279
BseBI CCWGG 3 cut(s) 45, 366, 683
BseDI CCNNGG 2 cut(s) 44, 338
BseGI GGATG 3 cut(s) 54, 348, 986
BseLI CCNNNNNNNGG 2 cut(s) 44, 479
BseMII CTCAG 8 cut(s) 422, 557, 684, 1044, 1245, 1363, 1644, 1692
BseNI ACTGG 4 cut(s) 304, 758, 836, 1279
BseRI GAGGAG 1 cut(s) 1226
BseSI GKGCMC 1 cut(s) 895
BseXI GCAGC 5 cut(s) 1636, 1639, 1642, 1645, 1697
BsgI GTGCAG 1 cut(s) 1601
BshFI GGCC 1 cut(s) 916
BshNI GGYRCC 1 cut(s) 1057
BsiHKAI GWGCWC 2 cut(s) 1103, 1631
BsiHKCI CYCGRG 1 cut(s) 1104
BsiSI CCGG 2 cut(s) 967, 1067
BslFI GGGAC 1 cut(s) 1324
BslI CCNNNNNNNGG 2 cut(s) 44, 479
BsmFI GGGAC 1 cut(s) 1324
BsmI GAATGC 1 cut(s) 1177
BsnI GGCC 1 cut(s) 916
BsoBI CYCGRG 1 cut(s) 1104
Bsp1286I GDGCHC 4 cut(s) 895, 1103, 1631, 1794
Bsp143I GATC 2 cut(s) 1279, 1325
BspANI GGCC 1 cut(s) 916
BspCNI CTCAG 8 cut(s) 421, 556, 685, 1045, 1244, 1362, 1643, 1693
BspHI TCATGA 1 cut(s) 1411
BspLI GGNNCC 2 cut(s) 786, 1059
BspMI ACCTGC 1 cut(s) 117
BspPI GGATC 2 cut(s) 1287, 1333
BspQI GCTCTTC 1 cut(s) 1091
BspT107I GGYRCC 1 cut(s) 1057
BsrI ACTGG 4 cut(s) 304, 758, 836, 1279
BssECI CCNNGG 2 cut(s) 44, 338
BssMI GATC 2 cut(s) 1279, 1325
BssNI GRCGYC 1 cut(s) 1756
BssT1I CCWWGG 1 cut(s) 338
Bst2UI CCWGG 3 cut(s) 45, 366, 683
Bst4CI ACNGT 4 cut(s) 439, 921, 1075, 1551
Bst6I CTCTTC 2 cut(s) 1036, 1091
BstACI GRCGYC 1 cut(s) 1756
BstC8I GCNNGC 3 cut(s) 1341, 1345, 1524
BstDEI CTNAG 8 cut(s) 408, 543, 693, 1053, 1231, 1349, 1630, 1701
BstEII GGTNACC 1 cut(s) 422
BstF5I GGATG 3 cut(s) 54, 348, 986
BstKTI GATC 2 cut(s) 1282, 1328
BstMBI GATC 2 cut(s) 1279, 1325
BstMWI GCNNNNNNNGC 4 cut(s) 270, 1655, 1658, 1753
BstNI CCWGG 3 cut(s) 45, 366, 683
BstPI GGTNACC 1 cut(s) 422
BstSCI CCNGG 4 cut(s) 43, 364, 681, 1065
BstSFI CTRYAG 1 cut(s) 1189
BstSLI GKGCMC 1 cut(s) 895
BstV1I GCAGC 5 cut(s) 1636, 1639, 1642, 1645, 1697
BstV2I GAAGAC 1 cut(s) 1104
BsuRI GGCC 1 cut(s) 916
BtsCI GGATG 3 cut(s) 54, 348, 986
BtsIMutI CAGTG 1 cut(s) 917
BveI ACCTGC 1 cut(s) 117
Cac8I GCNNGC 3 cut(s) 1341, 1345, 1524
CaiI CAGNNNCTG 1 cut(s) 1700
CciI TCATGA 1 cut(s) 1411
Cfr13I GGNCC 3 cut(s) 960, 969, 1691
CseI GACGC 2 cut(s) 1098, 1745
Csp6I GTAC 5 cut(s) 435, 547, 1027, 1058, 1537
CviAII CATG 1 cut(s) 1412
CviQI GTAC 5 cut(s) 435, 547, 1027, 1058, 1537
DdeI CTNAG 8 cut(s) 408, 543, 693, 1053, 1231, 1349, 1630, 1701
DpnI GATC 2 cut(s) 1281, 1327
DpnII GATC 2 cut(s) 1279, 1325
DraI TTTAAA 2 cut(s) 124, 511
Eam1104I CTCTTC 2 cut(s) 1036, 1091
EarI CTCTTC 2 cut(s) 1036, 1091
Ecl136II GAGCTC 2 cut(s) 1101, 1629
Eco130I CCWWGG 1 cut(s) 338
Eco24I GRGCYC 3 cut(s) 1103, 1631, 1794
Eco47I GGWCC 3 cut(s) 960, 969, 1691
Eco53kI GAGCTC 2 cut(s) 1101, 1629
Eco57I CTGAAG 3 cut(s) 827, 1104, 1608
Eco88I CYCGRG 1 cut(s) 1104
Eco91I GGTNACC 1 cut(s) 422
EcoICRI GAGCTC 2 cut(s) 1101, 1629
EcoO65I GGTNACC 1 cut(s) 422
EcoRI GAATTC 1 cut(s) 538
EcoRII CCWGG 3 cut(s) 43, 364, 681
EcoT14I CCWWGG 1 cut(s) 338
EcoT38I GRGCYC 3 cut(s) 1103, 1631, 1794
ErhI CCWWGG 1 cut(s) 338
FaeI CATG 1 cut(s) 1415
FaqI GGGAC 1 cut(s) 1324
FatI CATG 1 cut(s) 1411
FblI GTMKAC 1 cut(s) 1399
Fnu4HI GCNGC 5 cut(s) 1650, 1653, 1656, 1659, 1686
FokI GGATG 3 cut(s) 61, 355, 973
FriOI GRGCYC 3 cut(s) 1103, 1631, 1794
Fsp4HI GCNGC 5 cut(s) 1650, 1653, 1656, 1659, 1686
FspBI CTAG 5 cut(s) 339, 705, 800, 996, 1815
GluI GCNGC 5 cut(s) 1650, 1653, 1656, 1659, 1686
GsuI CTGGAG 1 cut(s) 775
HaeIII GGCC 1 cut(s) 916
HapII CCGG 2 cut(s) 967, 1067
HgaI GACGC 2 cut(s) 1098, 1745
Hin1I GRCGYC 1 cut(s) 1756
Hin1II CATG 1 cut(s) 1415
HincII GTYRAC 1 cut(s) 1039
HindII GTYRAC 1 cut(s) 1039
HpaI GTTAAC 1 cut(s) 1039
HpaII CCGG 2 cut(s) 967, 1067
HphI GGTGA 7 cut(s) 389, 569, 788, 819, 1005, 1463, 1733
Hpy166II GTNNAC 4 cut(s) 972, 1039, 1127, 1400
Hpy188III TCNNGA 8 cut(s) 182, 392, 491, 858, 1104, 1365, 1412, 1785
Hpy8I GTNNAC 4 cut(s) 972, 1039, 1127, 1400
HpyAV CCTTC 7 cut(s) 180, 840, 912, 973, 1128, 1537, 1708
HpyCH4III ACNGT 4 cut(s) 439, 921, 1075, 1551
HpyF10VI GCNNNNNNNGC 4 cut(s) 270, 1655, 1658, 1753
HpyF3I CTNAG 8 cut(s) 408, 543, 693, 1053, 1231, 1349, 1630, 1701
Hsp92I GRCGYC 1 cut(s) 1756
Hsp92II CATG 1 cut(s) 1415
KpnI GGTACC 1 cut(s) 1061
KspAI GTTAAC 1 cut(s) 1039
Kzo9I GATC 2 cut(s) 1279, 1325
LguI GCTCTTC 1 cut(s) 1091
LmnI GCTCC 5 cut(s) 184, 220, 790, 1004, 1106
Lsp1109I GCAGC 5 cut(s) 1636, 1639, 1642, 1645, 1697
LweI GCATC 4 cut(s) 138, 439, 833, 1778
MaeI CTAG 5 cut(s) 339, 705, 800, 996, 1815
MaeIII GTNAC 3 cut(s) 422, 700, 1551
MalI GATC 2 cut(s) 1281, 1327
MboI GATC 2 cut(s) 1279, 1325
MhlI GDGCHC 4 cut(s) 895, 1103, 1631, 1794
MlyI GAGTC 5 cut(s) 335, 722, 953, 1273, 1691
MmeI TCCRAC 1 cut(s) 380
MroXI GAANNNNTTC 1 cut(s) 1074
MseI TTAA 6 cut(s) 123, 510, 675, 792, 1038, 1566
MslI CAYNNNNRTG 1 cut(s) 1575
MspI CCGG 2 cut(s) 967, 1067
MspR9I CCNGG 4 cut(s) 45, 366, 683, 1067
Mva1269I GAATGC 1 cut(s) 1177
MvaI CCWGG 3 cut(s) 45, 366, 683
MwoI GCNNNNNNNGC 4 cut(s) 270, 1655, 1658, 1753
NciI CCSGG 1 cut(s) 1067
NdeII GATC 2 cut(s) 1279, 1325
NlaIII CATG 1 cut(s) 1415
NlaIV GGNNCC 2 cut(s) 786, 1059
NmeAIII GCCGAG 1 cut(s) 1773
PagI TCATGA 1 cut(s) 1411
PciSI GCTCTTC 1 cut(s) 1091
PctI GAATGC 1 cut(s) 1177
PdmI GAANNNNTTC 1 cut(s) 1074
PfeI GAWTC 6 cut(s) 8, 560, 1237, 1289, 1595, 1807
PflFI GACNNNGTC 1 cut(s) 1315
PflMI CCANNNNNTGG 1 cut(s) 479
PkrI GCNGC 5 cut(s) 1651, 1654, 1657, 1660, 1687
PleI GAGTC 5 cut(s) 334, 721, 952, 1272, 1691
PpsI GAGTC 5 cut(s) 334, 721, 952, 1272, 1691
PshBI ATTAAT 1 cut(s) 792
Psp124BI GAGCTC 2 cut(s) 1103, 1631
Psp6I CCWGG 3 cut(s) 43, 364, 681
PspEI GGTNACC 1 cut(s) 422
PspGI CCWGG 3 cut(s) 43, 364, 681
PspN4I GGNNCC 2 cut(s) 786, 1059
PspPI GGNCC 3 cut(s) 960, 969, 1691
PstNI CAGNNNCTG 1 cut(s) 1700
PsyI GACNNNGTC 1 cut(s) 1315
RsaI GTAC 5 cut(s) 436, 548, 1028, 1059, 1538
RsaNI GTAC 5 cut(s) 435, 547, 1027, 1058, 1537
RseI CAYNNNNRTG 1 cut(s) 1575
SacI GAGCTC 2 cut(s) 1103, 1631
SapI GCTCTTC 1 cut(s) 1091
SaqAI TTAA 6 cut(s) 123, 510, 675, 792, 1038, 1566
SatI GCNGC 5 cut(s) 1650, 1653, 1656, 1659, 1686
Sau3AI GATC 2 cut(s) 1279, 1325
Sau96I GGNCC 3 cut(s) 960, 969, 1691
ScaI AGTACT 3 cut(s) 436, 548, 1028
SchI GAGTC 5 cut(s) 335, 722, 953, 1273, 1691
ScrFI CCNGG 4 cut(s) 45, 366, 683, 1067
SduI GDGCHC 4 cut(s) 895, 1103, 1631, 1794
SfaNI GCATC 4 cut(s) 138, 439, 833, 1778
SfcI CTRYAG 1 cut(s) 1189
SinI GGWCC 3 cut(s) 960, 969, 1691
SmiMI CAYNNNNRTG 1 cut(s) 1575
SmlI CTYRAG 1 cut(s) 392
SmoI CTYRAG 1 cut(s) 392
SspMI CTAG 5 cut(s) 339, 705, 800, 996, 1815
SstI GAGCTC 2 cut(s) 1103, 1631
StyD4I CCNGG 4 cut(s) 43, 364, 681, 1065
StyI CCWWGG 1 cut(s) 338
TaaI ACNGT 4 cut(s) 439, 921, 1075, 1551
TaqI TCGA 7 cut(s) 207, 668, 851, 859, 1181, 1267, 1776
TaqII GACCGA 1 cut(s) 948
TatI WGTACW 3 cut(s) 434, 546, 1026
TfiI GAWTC 6 cut(s) 8, 560, 1237, 1289, 1595, 1807
Tru1I TTAA 6 cut(s) 123, 510, 675, 792, 1038, 1566
Tru9I TTAA 6 cut(s) 123, 510, 675, 792, 1038, 1566
TscAI CASTG 1 cut(s) 924
TseI GCWGC 5 cut(s) 1649, 1652, 1655, 1658, 1685
TspDTI ATGAA 7 cut(s) 65, 218, 229, 372, 726, 945, 1067
TspRI CASTG 1 cut(s) 924
Tth111I GACNNNGTC 1 cut(s) 1315
Van91I CCANNNNNTGG 1 cut(s) 479
VpaK11BI GGWCC 3 cut(s) 960, 969, 1691
VspI ATTAAT 1 cut(s) 792
XapI RAATTY 6 cut(s) 185, 412, 496, 538, 806, 1736
XmaJI CCTAGG 1 cut(s) 338
XmiI GTMKAC 1 cut(s) 1399
XmnI GAANNNNTTC 1 cut(s) 1074
XspI CTAG 5 cut(s) 339, 705, 800, 996, 1815
ZrmI AGTACT 3 cut(s) 436, 548, 1028
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.