Rw1G020630

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0007611
Physical Location & Seq
Reverse (-)
44232419 .. 44233258
840 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G020630.1

Sequence Viewer

Length: 840 bp
ATGGAAGAGAAACCCTCAAAAACCCTCAATTTGATCGACAAGTTAATGCATGCAGCAGAATCACCCTCAACTGCTAGTAGTAGCAGAACCAGTACTGCAGGAGACAACCAACGACTCTGTGATGCAGCCACTGCTTTAACTTTGCATCAATCTTGGTTGCAGCCGGCATCGACCACAAACAAGAGAAAAGCAAAAGCACCAGTTATACCGGAAGAGGAAGACAGAATCGAGAAACCAGCCGCTCCTACTAATCTGAAACATCAAAAACTAGTGCTCTCTGATCATGAGAATATGGAGCCAAAGACGAAAAGTGACCCGGTAGCGGAATGTGATGATGCTCAGAACCTTCATACACTAACTGAAGGGAGCGCTAGGCCGAGTGCTCATCATCATATTGATGAGCAGGTGGCTCGTGACCCGGCCTTATACAAAATAAAGAAGAAGTTGACTCTAAGTGACCTCGGAAACTTGAGCAGGCTCTTGGTGCCAAAAGAGATGGTCAAGAACCATGTTCTGCCTCACGTGAATGGCGACTTTGTACAAAGACTCGAGAGCAAAGCCGGTAAACAAGTTACGGTTAAAGACTCTGACAAAGAGACCGAGCATCAATTAACTTTTAGATACTTAAGGTCGTCGAAGAGTTATGTTTTCAATGGAAATTGGATGGAAATCGTGAAGGGGAGAGATTTGAAGAAGGATGATGAAATTGGACTTTACTGGGACACTCTCAATTCCAATTTCCACTTTTCTCTTCTGAAACTTGCAAGCCAAGAAAGCCAAGCTGCCGATGAAGTTACTCGCGCGTGTTCATCAACAATGACATCAAGTCTAGAATCTTAG

Protein Analysis

279

Amino Acids

31.18

Weight (kDa)

6.64

Isoelectric Point (pI)

46.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 147 - 242 4.4e-07 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 394
Acc36I ACCTGC 1 cut(s) 394
AccB1I GGYRCC 1 cut(s) 484
AccBSI CCGCTC 1 cut(s) 242
AccII CGCG 2 cut(s) 801, 803
AciI CCGC 2 cut(s) 240, 323
AcuI CTGAAG 1 cut(s) 381
AcvI CACGTG 1 cut(s) 523
AfaI GTAC 2 cut(s) 94, 540
AfeI AGCGCT 1 cut(s) 370
AfiI CCNNNNNNNGG 1 cut(s) 322
AflII CTTAAG 1 cut(s) 625
AgsI TTSAA 2 cut(s) 652, 691
AhdI GACNNNNNGTC 1 cut(s) 825
AhlI ACTAGT 1 cut(s) 268
AluBI AGCT 1 cut(s) 782
AluI AGCT 1 cut(s) 782
Alw21I GWGCWC 2 cut(s) 276, 385
Alw26I GTCTC 2 cut(s) 96, 590
AlwNI CAGNNNCTG 1 cut(s) 131
Ama87I CYCGRG 1 cut(s) 548
Aor51HI AGCGCT 1 cut(s) 370
AoxI GGCC 2 cut(s) 374, 420
ApeKI GCWGC 4 cut(s) 53, 125, 160, 782
AspLEI GCGC 2 cut(s) 371, 803
AsuC2I CCSGG 2 cut(s) 317, 419
AsuHPI GGTGA 1 cut(s) 54
AvaI CYCGRG 1 cut(s) 548
BanI GGYRCC 1 cut(s) 484
BauI CACGAG 1 cut(s) 411
BbrPI CACGTG 1 cut(s) 523
BbsI GAAGAC 1 cut(s) 225
Bbv12I GWGCWC 2 cut(s) 276, 385
BbvI GCAGC 4 cut(s) 65, 137, 172, 769
BccI CCATC 2 cut(s) 490, 658
BcgI CGANNNNNNTGC 2 cut(s) 392, 426
BclI TGATCA 1 cut(s) 280
BcnI CCSGG 2 cut(s) 317, 419
BcoDI GTCTC 2 cut(s) 96, 590
BcuI ACTAGT 1 cut(s) 268
BfaI CTAG 4 cut(s) 75, 269, 372, 830
BfmI CTRYAG 1 cut(s) 96
BfoI RGCGCY 1 cut(s) 372
BfrI CTTAAG 1 cut(s) 625
BfuAI ACCTGC 1 cut(s) 394
BisI GCNGC 5 cut(s) 54, 126, 161, 240, 783
BlsI GCNGC 5 cut(s) 55, 127, 162, 241, 784
BmcAI AGTACT 1 cut(s) 94
Bme1390I CCNGG 2 cut(s) 317, 419
BmeRI GACNNNNNGTC 1 cut(s) 825
BmeT110I CYCGRG 1 cut(s) 548
BmiI GGNNCC 2 cut(s) 297, 486
BmrFI CCNGG 2 cut(s) 317, 419
BmrI ACTGGG 1 cut(s) 727
BmsI GCATC 5 cut(s) 112, 154, 176, 325, 613
BmuI ACTGGG 1 cut(s) 727
BpiI GAAGAC 1 cut(s) 225
BplI GAGNNNNNCTC 1 cut(s) 31
BpuEI CTTGAG 1 cut(s) 490
BpuMI CCSGG 2 cut(s) 317, 419
BsaAI YACGTR 1 cut(s) 523
BsaBI GATNNNNATC 1 cut(s) 668
BsaI GGTCTC 1 cut(s) 590
BsaJI CCNNGG 1 cut(s) 460
BsaWI WCCGGW 1 cut(s) 208
Bsc4I CCNNNNNNNGG 1 cut(s) 322
Bse118I RCCGGY 2 cut(s) 163, 560
Bse1I ACTGG 3 cut(s) 90, 200, 722
Bse8I GATNNNNATC 1 cut(s) 668
BseDI CCNNGG 1 cut(s) 460
BseGI GGATG 2 cut(s) 669, 703
BseJI GATNNNNATC 1 cut(s) 668
BseLI CCNNNNNNNGG 1 cut(s) 322
BseMII CTCAG 1 cut(s) 353
BseNI ACTGG 3 cut(s) 90, 200, 722
BseXI GCAGC 4 cut(s) 65, 137, 172, 769
Bsh1236I CGCG 2 cut(s) 801, 803
BshFI GGCC 2 cut(s) 376, 422
BshNI GGYRCC 1 cut(s) 484
BsiHKAI GWGCWC 2 cut(s) 276, 385
BsiHKCI CYCGRG 1 cut(s) 548
BsiSI CCGG 5 cut(s) 164, 209, 317, 419, 561
BslFI GGGAC 1 cut(s) 734
BslI CCNNNNNNNGG 1 cut(s) 322
BsmAI GTCTC 2 cut(s) 96, 590
BsmFI GGGAC 1 cut(s) 734
BsnI GGCC 2 cut(s) 376, 422
Bso31I GGTCTC 1 cut(s) 590
BsoBI CYCGRG 1 cut(s) 548
Bsp1286I GDGCHC 2 cut(s) 276, 385
Bsp1407I TGTACA 1 cut(s) 538
Bsp143I GATC 2 cut(s) 33, 280
BspACI CCGC 2 cut(s) 240, 323
BspANI GGCC 2 cut(s) 376, 422
BspCNI CTCAG 1 cut(s) 352
BspFNI CGCG 2 cut(s) 801, 803
BspHI TCATGA 1 cut(s) 283
BspLI GGNNCC 2 cut(s) 297, 486
BspMAI CTGCAG 1 cut(s) 100
BspMI ACCTGC 1 cut(s) 394
BspT107I GGYRCC 1 cut(s) 484
BspTI CTTAAG 1 cut(s) 625
BspTNI GGTCTC 1 cut(s) 590
BsrBI CCGCTC 1 cut(s) 242
BsrFI RCCGGY 2 cut(s) 163, 560
BsrGI TGTACA 1 cut(s) 538
BsrI ACTGG 3 cut(s) 90, 200, 722
BssAI RCCGGY 2 cut(s) 163, 560
BssECI CCNNGG 1 cut(s) 460
BssMI GATC 2 cut(s) 33, 280
BssSI CACGAG 1 cut(s) 411
Bst2BI CACGAG 1 cut(s) 411
Bst4CI ACNGT 1 cut(s) 577
Bst6I CTCTTC 3 cut(s) 207, 632, 756
BstAFI CTTAAG 1 cut(s) 625
BstAPI GCANNNNNTGC 1 cut(s) 131
BstAUI TGTACA 1 cut(s) 538
BstBAI YACGTR 1 cut(s) 523
BstC8I GCNNGC 4 cut(s) 51, 165, 476, 766
BstDEI CTNAG 3 cut(s) 339, 452, 837
BstF5I GGATG 2 cut(s) 669, 703
BstFNI CGCG 2 cut(s) 801, 803
BstH2I RGCGCY 1 cut(s) 372
BstHHI GCGC 2 cut(s) 371, 803
BstKTI GATC 2 cut(s) 36, 283
BstMAI GTCTC 2 cut(s) 96, 590
BstMBI GATC 2 cut(s) 33, 280
BstMWI GCNNNNNNNGC 3 cut(s) 131, 484, 774
BstNSI RCATGY 1 cut(s) 53
BstSCI CCNGG 2 cut(s) 315, 417
BstSFI CTRYAG 1 cut(s) 96
BstUI CGCG 2 cut(s) 801, 803
BstV1I GCAGC 4 cut(s) 65, 137, 172, 769
BstV2I GAAGAC 1 cut(s) 225
BsuRI GGCC 2 cut(s) 376, 422
BtsCI GGATG 2 cut(s) 669, 703
BtsI GCAGTG 1 cut(s) 129
BtsIMutI CAGTG 1 cut(s) 129
BveI ACCTGC 1 cut(s) 394
Cac8I GCNNGC 4 cut(s) 51, 165, 476, 766
CaiI CAGNNNCTG 1 cut(s) 131
CciI TCATGA 1 cut(s) 283
CfoI GCGC 2 cut(s) 371, 803
Cfr10I RCCGGY 2 cut(s) 163, 560
Csp6I GTAC 2 cut(s) 93, 539
CviAII CATG 3 cut(s) 50, 284, 509
CviQI GTAC 2 cut(s) 93, 539
DdeI CTNAG 3 cut(s) 339, 452, 837
DpnI GATC 2 cut(s) 35, 282
DpnII GATC 2 cut(s) 33, 280
DriI GACNNNNNGTC 1 cut(s) 825
Eam1104I CTCTTC 3 cut(s) 207, 632, 756
Eam1105I GACNNNNNGTC 1 cut(s) 825
EarI CTCTTC 3 cut(s) 207, 632, 756
Eco31I GGTCTC 1 cut(s) 590
Eco47III AGCGCT 1 cut(s) 370
Eco57I CTGAAG 1 cut(s) 381
Eco72I CACGTG 1 cut(s) 523
Eco88I CYCGRG 1 cut(s) 548
EcoT22I ATGCAT 1 cut(s) 51
FaeI CATG 3 cut(s) 53, 287, 512
FaiI YATR 9 cut(s) 51, 206, 285, 293, 351, 393, 427, 510, 645
FaqI GGGAC 1 cut(s) 734
FatI CATG 3 cut(s) 49, 283, 508
FbaI TGATCA 1 cut(s) 280
Fnu4HI GCNGC 5 cut(s) 54, 126, 161, 240, 783
FokI GGATG 2 cut(s) 676, 710
Fsp4HI GCNGC 5 cut(s) 54, 126, 161, 240, 783
FspBI CTAG 4 cut(s) 75, 269, 372, 830
GlaI GCGC 2 cut(s) 370, 802
GluI GCNGC 5 cut(s) 54, 126, 161, 240, 783
HaeII RGCGCY 1 cut(s) 372
HaeIII GGCC 2 cut(s) 376, 422
HapII CCGG 5 cut(s) 164, 209, 317, 419, 561
HhaI GCGC 2 cut(s) 371, 803
Hin1II CATG 3 cut(s) 53, 287, 512
Hin6I GCGC 2 cut(s) 369, 801
HinP1I GCGC 2 cut(s) 369, 801
HincII GTYRAC 1 cut(s) 447
HindII GTYRAC 1 cut(s) 447
HinfI GANTC 7 cut(s) 59, 114, 225, 448, 546, 584, 833
HpaII CCGG 5 cut(s) 164, 209, 317, 419, 561
HphI GGTGA 1 cut(s) 54
Hpy166II GTNNAC 2 cut(s) 447, 566
Hpy188I TCNGA 6 cut(s) 255, 280, 342, 464, 589, 756
Hpy188III TCNNGA 7 cut(s) 229, 284, 413, 502, 550, 673, 830
Hpy8I GTNNAC 2 cut(s) 447, 566
Hpy99I CGWCG 1 cut(s) 637
HpyAV CCTTC 4 cut(s) 356, 356, 670, 688
HpyCH4III ACNGT 1 cut(s) 577
HpyCH4IV ACGT 1 cut(s) 522
HpyCH4V TGCA 7 cut(s) 49, 53, 98, 125, 145, 160, 764
HpyF10VI GCNNNNNNNGC 3 cut(s) 131, 484, 774
HpyF3I CTNAG 3 cut(s) 339, 452, 837
HpySE526I ACGT 1 cut(s) 522
Hsp92II CATG 3 cut(s) 53, 287, 512
HspAI GCGC 2 cut(s) 369, 801
KroI GCCGGC 1 cut(s) 163
KroNI GCCGGC 1 cut(s) 165
Ksp22I TGATCA 1 cut(s) 280
Kzo9I GATC 2 cut(s) 33, 280
LmnI GCTCC 3 cut(s) 247, 295, 366
Lsp1109I GCAGC 4 cut(s) 65, 137, 172, 769
LweI GCATC 5 cut(s) 112, 154, 176, 325, 613
MaeI CTAG 4 cut(s) 75, 269, 372, 830
MaeII ACGT 1 cut(s) 522
MaeIII GTNAC 5 cut(s) 311, 413, 455, 571, 793
MalI GATC 2 cut(s) 35, 282
MbiI CCGCTC 1 cut(s) 242
MboI GATC 2 cut(s) 33, 280
MboII GAAGA 7 cut(s) 17, 224, 230, 451, 649, 703, 743
MhlI GDGCHC 2 cut(s) 276, 385
MluCI AATT 6 cut(s) 28, 608, 658, 705, 730, 736
MlyI GAGTC 4 cut(s) 108, 442, 540, 578
MnlI CCTC 6 cut(s) 25, 35, 76, 208, 470, 528
Mph1103I ATGCAT 1 cut(s) 51
MroNI GCCGGC 1 cut(s) 163
MseI TTAA 5 cut(s) 44, 137, 579, 611, 626
MslI CAYNNNNRTG 2 cut(s) 396, 525
MspCI CTTAAG 1 cut(s) 625
MspI CCGG 5 cut(s) 164, 209, 317, 419, 561
MspR9I CCNGG 2 cut(s) 317, 419
MvnI CGCG 2 cut(s) 801, 803
MwoI GCNNNNNNNGC 3 cut(s) 131, 484, 774
NaeI GCCGGC 1 cut(s) 165
NciI CCSGG 2 cut(s) 317, 419
NdeII GATC 2 cut(s) 33, 280
NgoMIV GCCGGC 1 cut(s) 163
NlaIII CATG 3 cut(s) 53, 287, 512
NlaIV GGNNCC 2 cut(s) 297, 486
NmeAIII GCCGAG 1 cut(s) 402
NmuCI GTSAC 3 cut(s) 311, 413, 455
NsiI ATGCAT 1 cut(s) 51
NspI RCATGY 1 cut(s) 53
PaeI GCATGC 1 cut(s) 53
PaeR7I CTCGAG 1 cut(s) 548
PagI TCATGA 1 cut(s) 283
PaqCI CACCTGC 1 cut(s) 394
PcsI WCGNNNNNNNCGW 1 cut(s) 528
PdiI GCCGGC 1 cut(s) 165
PfeI GAWTC 3 cut(s) 59, 225, 833
PkrI GCNGC 5 cut(s) 55, 127, 162, 241, 784
PleI GAGTC 4 cut(s) 108, 442, 540, 578
PmaCI CACGTG 1 cut(s) 523
PmlI CACGTG 1 cut(s) 523
PpsI GAGTC 4 cut(s) 108, 442, 540, 578
Ppu21I YACGTR 1 cut(s) 523
PspCI CACGTG 1 cut(s) 523
PspN4I GGNNCC 2 cut(s) 297, 486
PstI CTGCAG 1 cut(s) 100
PstNI CAGNNNCTG 1 cut(s) 131
RsaI GTAC 2 cut(s) 94, 540
RsaNI GTAC 2 cut(s) 93, 539
RseI CAYNNNNRTG 2 cut(s) 396, 525
SaqAI TTAA 5 cut(s) 44, 137, 579, 611, 626
SatI GCNGC 5 cut(s) 54, 126, 161, 240, 783
Sau3AI GATC 2 cut(s) 33, 280
ScaI AGTACT 1 cut(s) 94
SchI GAGTC 4 cut(s) 108, 442, 540, 578
ScrFI CCNGG 2 cut(s) 317, 419
SduI GDGCHC 2 cut(s) 276, 385
SetI ASST 6 cut(s) 348, 408, 462, 525, 632, 784
SfaNI GCATC 5 cut(s) 112, 154, 176, 325, 613
SfcI CTRYAG 1 cut(s) 96
Sfr274I CTCGAG 1 cut(s) 548
SlaI CTCGAG 1 cut(s) 548
SmiMI CAYNNNNRTG 2 cut(s) 396, 525
SmlI CTYRAG 3 cut(s) 469, 548, 625
SmoI CTYRAG 3 cut(s) 469, 548, 625
SpeI ACTAGT 1 cut(s) 268
SphI GCATGC 1 cut(s) 53
Sse9I AATT 6 cut(s) 28, 608, 658, 705, 730, 736
SsiI CCGC 2 cut(s) 240, 323
SspMI CTAG 4 cut(s) 75, 269, 372, 830
StyD4I CCNGG 2 cut(s) 315, 417
TaaI ACNGT 1 cut(s) 577
TaiI ACGT 1 cut(s) 525
TaqI TCGA 5 cut(s) 36, 170, 228, 549, 635
TaqII GACCGA 1 cut(s) 614
TasI AATT 6 cut(s) 28, 608, 658, 705, 730, 736
TatI WGTACW 2 cut(s) 92, 538
TauI GCSGC 1 cut(s) 242
TfiI GAWTC 3 cut(s) 59, 225, 833
Tru1I TTAA 5 cut(s) 44, 137, 579, 611, 626
Tru9I TTAA 5 cut(s) 44, 137, 579, 611, 626
TscAI CASTG 1 cut(s) 136
TseFI GTSAC 3 cut(s) 311, 413, 455
TseI GCWGC 4 cut(s) 53, 125, 160, 782
Tsp45I GTSAC 3 cut(s) 311, 413, 455
TspDTI ATGAA 4 cut(s) 338, 717, 798, 804
TspRI CASTG 1 cut(s) 136
Vha464I CTTAAG 1 cut(s) 625
XbaI TCTAGA 1 cut(s) 829
XceI RCATGY 1 cut(s) 53
XhoI CTCGAG 1 cut(s) 548
XspI CTAG 4 cut(s) 75, 269, 372, 830
ZrmI AGTACT 1 cut(s) 94
Zsp2I ATGCAT 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.