Rorug05G0480500

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0007611
Physical Location & Seq
Forward (+)
66195460 .. 66195893
434 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0480500.1

Sequence Viewer

Length: 333 bp
ATGTCTTGCCCTCATGCTGCTGCTGTTGCTGCGTATGTTAAATCATTCCATCCCGATTGGTCTTCATCAGCTATAAAATCATCTCTTATGACTACTGCTTGGCCAATGAATAATACCAACAATGCACCTGCTGAGTTTGCTTATGGATCTGGACATGTCAATCCTGTAAAAGCTATAGATCCCGGGCTTGTTTATGAAACTTCTAAAGAAGATTTCATAAACCTACTTTGCATGGACTATGATGAAGCCAAAGTGAGACTGATATCAGGAGATAACAGCACTTGCCCTACAGGCTCTGACAAAGGATCACCAAAGGATCTTAACTACCCTTAA

Protein Analysis

110

Amino Acids

11.83

Weight (kDa)

5.11

Isoelectric Point (pI)

31.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 1 - 51 2.9e-07 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 136
Acc36I ACCTGC 1 cut(s) 136
AclWI GGATC 4 cut(s) 154, 173, 313, 324
AcoI YGGCCR 1 cut(s) 101
AflIII ACRYGT 1 cut(s) 154
AluBI AGCT 2 cut(s) 71, 173
AluI AGCT 2 cut(s) 71, 173
Alw26I GTCTC 1 cut(s) 250
AlwI GGATC 4 cut(s) 154, 173, 313, 324
AlwNI CAGNNNCTG 1 cut(s) 296
Ama87I CYCGRG 1 cut(s) 182
AoxI GGCC 1 cut(s) 101
ApeKI GCWGC 3 cut(s) 17, 20, 29
AsuC2I CCSGG 2 cut(s) 183, 184
AsuHPI GGTGA 1 cut(s) 300
AvaI CYCGRG 1 cut(s) 182
BalI TGGCCA 1 cut(s) 103
BbsI GAAGAC 1 cut(s) 54
BbvI GCAGC 3 cut(s) 4, 7, 16
BccI CCATC 1 cut(s) 57
BcnI CCSGG 2 cut(s) 183, 184
BcoDI GTCTC 1 cut(s) 250
BfmI CTRYAG 2 cut(s) 174, 288
BfuAI ACCTGC 1 cut(s) 136
BglI GCCNNNNNGGC 1 cut(s) 291
BisI GCNGC 3 cut(s) 18, 21, 30
BlsI GCNGC 3 cut(s) 19, 22, 31
Bme1390I CCNGG 2 cut(s) 183, 184
BmeT110I CYCGRG 1 cut(s) 182
BmrFI CCNGG 2 cut(s) 183, 184
BpiI GAAGAC 1 cut(s) 54
BpuMI CCSGG 2 cut(s) 183, 184
BsaJI CCNNGG 1 cut(s) 182
BseDI CCNNGG 1 cut(s) 182
BseGI GGATG 1 cut(s) 49
BseMII CTCAG 1 cut(s) 123
BseXI GCAGC 3 cut(s) 4, 7, 16
BshFI GGCC 1 cut(s) 103
BsiHKCI CYCGRG 1 cut(s) 182
BsiSI CCGG 1 cut(s) 183
BsmAI GTCTC 1 cut(s) 250
BsnI GGCC 1 cut(s) 103
BsoBI CYCGRG 1 cut(s) 182
Bsp143I GATC 4 cut(s) 146, 178, 305, 316
BspANI GGCC 1 cut(s) 103
BspCNI CTCAG 1 cut(s) 124
BspMI ACCTGC 1 cut(s) 136
BspPI GGATC 4 cut(s) 154, 173, 313, 324
BssECI CCNNGG 1 cut(s) 182
BssMI GATC 4 cut(s) 146, 178, 305, 316
BstDEI CTNAG 1 cut(s) 132
BstF5I GGATG 1 cut(s) 49
BstKTI GATC 4 cut(s) 149, 181, 308, 319
BstMAI GTCTC 1 cut(s) 250
BstMBI GATC 4 cut(s) 146, 178, 305, 316
BstMWI GCNNNNNNNGC 4 cut(s) 26, 29, 137, 291
BstNSI RCATGY 1 cut(s) 158
BstSCI CCNGG 2 cut(s) 181, 182
BstSFI CTRYAG 2 cut(s) 174, 288
BstV1I GCAGC 3 cut(s) 4, 7, 16
BstV2I GAAGAC 1 cut(s) 54
BstX2I RGATCY 3 cut(s) 146, 178, 316
BstYI RGATCY 3 cut(s) 146, 178, 316
BsuRI GGCC 1 cut(s) 103
BtsCI GGATG 1 cut(s) 49
BveI ACCTGC 1 cut(s) 136
CaiI CAGNNNCTG 1 cut(s) 296
Cfr9I CCCGGG 1 cut(s) 182
CviAII CATG 3 cut(s) 14, 155, 232
CviJI RGCY 6 cut(s) 71, 103, 173, 187, 248, 294
CviKI_1 RGCY 6 cut(s) 71, 103, 173, 187, 248, 294
DdeI CTNAG 1 cut(s) 132
DpnI GATC 4 cut(s) 148, 180, 307, 318
DpnII GATC 4 cut(s) 146, 178, 305, 316
EaeI YGGCCR 1 cut(s) 101
Eco32I GATATC 1 cut(s) 264
Eco88I CYCGRG 1 cut(s) 182
EcoRV GATATC 1 cut(s) 264
FaeI CATG 3 cut(s) 17, 158, 235
FatI CATG 3 cut(s) 13, 154, 231
Fnu4HI GCNGC 3 cut(s) 18, 21, 30
FokI GGATG 1 cut(s) 36
Fsp4HI GCNGC 3 cut(s) 18, 21, 30
GluI GCNGC 3 cut(s) 18, 21, 30
HaeIII GGCC 1 cut(s) 103
HapII CCGG 1 cut(s) 183
Hin1II CATG 3 cut(s) 17, 158, 235
HpaII CCGG 1 cut(s) 183
HphI GGTGA 1 cut(s) 300
Hpy188I TCNGA 1 cut(s) 298
Hpy188III TCNNGA 3 cut(s) 53, 150, 267
HpyCH4V TGCA 2 cut(s) 125, 231
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 29, 137, 291
HpyF3I CTNAG 1 cut(s) 132
Hsp92II CATG 3 cut(s) 17, 158, 235
Kzo9I GATC 4 cut(s) 146, 178, 305, 316
LpnPI CCDG 6 cut(s) 135, 141, 177, 196, 252, 276
Lsp1109I GCAGC 3 cut(s) 4, 7, 16
MalI GATC 4 cut(s) 148, 180, 307, 318
MboI GATC 4 cut(s) 146, 178, 305, 316
MboII GAAGA 2 cut(s) 54, 221
MflI RGATCY 3 cut(s) 146, 178, 316
MlsI TGGCCA 1 cut(s) 103
MluNI TGGCCA 1 cut(s) 103
MnlI CCTC 1 cut(s) 21
Mox20I TGGCCA 1 cut(s) 103
MscI TGGCCA 1 cut(s) 103
MseI TTAA 3 cut(s) 39, 321, 331
Msp20I TGGCCA 1 cut(s) 103
MspI CCGG 1 cut(s) 183
MspR9I CCNGG 2 cut(s) 183, 184
MwoI GCNNNNNNNGC 4 cut(s) 26, 29, 137, 291
NciI CCSGG 2 cut(s) 183, 184
NdeII GATC 4 cut(s) 146, 178, 305, 316
NlaIII CATG 3 cut(s) 17, 158, 235
NspI RCATGY 1 cut(s) 158
PaqCI CACCTGC 1 cut(s) 136
PciI ACATGT 1 cut(s) 154
PkrI GCNGC 3 cut(s) 19, 22, 31
PscI ACATGT 1 cut(s) 154
PstNI CAGNNNCTG 1 cut(s) 296
PsuI RGATCY 3 cut(s) 146, 178, 316
SaqAI TTAA 3 cut(s) 39, 321, 331
SatI GCNGC 3 cut(s) 18, 21, 30
Sau3AI GATC 4 cut(s) 146, 178, 305, 316
ScrFI CCNGG 2 cut(s) 183, 184
SetI ASST 4 cut(s) 73, 130, 175, 225
SfcI CTRYAG 2 cut(s) 174, 288
SmaI CCCGGG 1 cut(s) 184
StyD4I CCNGG 2 cut(s) 181, 182
Tru1I TTAA 3 cut(s) 39, 321, 331
Tru9I TTAA 3 cut(s) 39, 321, 331
TseI GCWGC 3 cut(s) 17, 20, 29
TspDTI ATGAA 5 cut(s) 54, 122, 205, 210, 258
TspMI CCCGGG 1 cut(s) 182
XceI RCATGY 1 cut(s) 158
XmaI CCCGGG 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.