FvH4_7g20250

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003478
Physical Location & Seq
Reverse (-)
16491670 .. 16497648
5979 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g20250.t1

Sequence Viewer

Length: 3351 bp
ATGGACGAGAGCAAAGAGAAGGGTGTTAGTGCTGCTGGGGAAGTTGTAGCTGAGGAAGGTGGGTCTTGTAATGGGGATGAACTAATGGTGGAGGTTTTCGGGTCTAATGTGTATGTGGGTGGAGTTTGTACTAGCGGAGGAGGAGATGAGGAAGGTGAGGAGGTGGGCCGCGAGGAGTCGGTTGAGGTTGAGATGGGTTCGGGTGGGAAGGCGGAGGCGGTGGAGGAGAGTTTGAAGGGAGCAGGGGCAGCTGGTGGTGCTGGTTCAGCTGTTGATGCGGAGAAAGCTGAAAAGGAAAGGAAACTGATTGGAGGACTTCTGGAGGAAGTTTTTGGGCGTGGGCTTATGGATGGTGGGAGAAGAGAAAAGAAGGGAGTTGGTGGTGGGGCTGATTTGAAGAAAGCTAGTGGTGCAAGTTCTTCGGAAACAGTGGAGAATTCTCGTGTTCAGACCCAAGTTGTGGAGAAAGCTTCAGTTGTTAGTGAAGTAGGTTCGAGTCCCAGAGTCTTGAATGGACAAAGGCAAGTTTCTGCTGGTGGTGGTGATGTTTCAGGCGCGGTCAAAGGAGATACCGTGACTTCTGAAGTTGCAGGAGTAGAAACTGAGAAGATTCGTGGAAGTCATTCAGTAGGAAAACAGCAGGTTAAGATCGAGTCACTAGGTGGAAACATCCATACTCATGTCAATGTACCTGCACATACAGTTGCCTCATCCCTGCCGGATGGAGAGGATCTCTTGAATTCTGAAAAAGATCATTTGAAACTAGATGAGAGTGTAGAGAACACTTTTCATGATATTGCTCAGGTTGGGTCAACTACATCTCTGCCAGCTAAACTACTTCCTGGATGCGTAGATCAAGAGGGTCTTTTGAAGTTTGAAAGTCATCAGCACTTGAAAGCCGAAGATATCATAGACAAGAACAGGGTCCATGATATGGCCCGGGTTGAATTAAATGGACGGCAAGAAATGGAAGTGGACAATCAAGTTACTGATGCTGATCATGGTGAGTTGGTTTCAGGAATTGGTCAGAACCCAAAAATAGAGAAGGCATGCATGAACTCTGTTGGGAAGGATCAAAAGTTGAAGTCTGAAGAAAGTTTAAACAATAATGTGACCACTGATCTTGGTCAGTCCAAGACCAATGCTGTACATGAGGTTGAGGTAACTGAAGCTGATATGGATGGTTTATATGGAGAAACACAAGACATGGAAGTGGAAAGAGATGCTAGTCATATGGAACAGCTCAAACCTAGTGAAGAGAAAACTGTTATTCGAGAAGCTACGCAGCCTGAAACCTCTGAAATAGTGCCTGAACTTAGATATGAGCTGCCTCAAAAACATGAAGGTACATTTTCTGTCTCAGATTTAGTTTGGGGCAAAGTGAAGAGCCATCCATGGTGGCCTGGCCAGATATTTGATTTTATGGCTGCATCTGAGAAGGCGATGAAACATCATAAGAAGGACTGTTTTTTGGTAGCATATTTTGGGGACCGAACATTTGCTTGGAACGAAGTATCTAGTTTAAAGCCTTTCCAATCTTATTTCTCCCAGGCACAGAAGCAGTGCACTTCCGAAACATTCCACAAAGCTGTCAATTGTGTTCTGGAAGAAGTTTCAAGACGTGTAGAATTGGGGTTATCATGCTCATGCATACCAAAAGATGTTTATGAAAAGATTAGATTCCAGATTGTTGAAAATGCTGGGATATGCAGTGAATCGAGTAGAGTGGAAGGAGTGGATGAATCTGCAAGTGCAAGTTCATTTGAATCCGATAAGCTACTTACATATGTAAAAGCTTTAGCACGGTTTCCATCTGGTAGAAGCGAAAAATTAGAACTTGTCATTGCCAAGGCTCACTTGACGTCATTCTTCCGTTCAAAGGGTTATTGTAGTTTGCCTGAGTTCCAATTTTGTGGAAATTTGTTGGAAAGTGAAACAGATAATTCATTCTCTGAGGGCAAAACATGTCCAGGTGAAATTACTGAGCATGCGACTTCTATTGGGAAGGATAAGAAGACTGGCCCGGAGGTGGAGGAACTCAAGTCTAGCTCTTCTCATAAACGTAAGCATAATCTAAGAGAAGGAGCGTATGCTAAAATGAAAGAAAGAAGCATGTCAGAGTTAATGGGTGCCGAAGATGGTAATGATTGGTTCGATGTGAAGGCTTTACCATCATCTGCCAAGAGACGGAAGGGTGCTGACTTGGCCACACAAGATGGGAGGAAAGCTGTTTCACCTCTACCAAAACCATCATTTAAGATTGGTGAATGTATCCAGAGAGCAGCAAGCCAACTGTCAGGGTCTACTATAGTGAAGTCCAGCACCGACAGACCTGCTGTGCAGGGATCTGATGTTTCCTTCCAGAATTCGGATGATACTCTTAGAGGAGTGAATAATACAACAAAGTACTCATCTCTAGATGAACTGCTGTCACAACTTCGGTTGGCAGCGGAAGAACCCCTGAAGGAATACAATTCCTTGAGTACTATTGTCAATTTCTTCTCTGATTTTAGAAATTCTGTGGTTGTTGGCCAGAAATCTGGGTTGGGGCTCTTGGTTGTAGACAAAGTCGGTGGTAGAAAGAGGAAGTTAAATTCTGTGCTTGGGTCACCTGAGACTTTTGAATTTGATGACATGAATGACACTTATTGGACAGACATGGTAATCCAAAATGGTGGAGAAGAGGAAGCTCCACGGAAAAGAAAACCAAAGTATCAAGCTGTCGTCCTTGGGCAACCAGAAAAGCCCGCACAAGTGGGTCGCAGGCCATACACCAGGAAGAAGTTTTCTCAAGGGAGTCAAGATTTGCCTCCGGAGAAGCCTGTTGGTTATGTCGATGAGAATGCCCCAGCAGAACTTGTAATGAGCTTCTCTGAGGTGAGCTCTATTCCTTCAGAAACAAACCTGAATAAAATGTTCAAGCGCTTTGGACCTCTGAAGGAATATGAAACAGAAGTTGATAGGGAGAGCAGCCGTGCTAGAGTGGTTTTCAAGAGATGTTCTGATGCTGAAGTTGCTTGCAATAGTGCTGGGAAATTCAATATCTTTGGACAGATTACTGTAAGTTACCAGCTCAACTACACTCCCTCTCAGCTCAACTACACGCCATCTATTACGTTTGGTGCATCTACCAGTGGTGCACCCCAGGATCAGGAGGTGCAACTTGTTTTTCCCTCCCATGATCATGAGATGCATCTTGATCTCTCCACCCATGACCAGATGCAGCTTGATCTTTCCACCCATGACCAGATGCAGCTTGACCTTTCCACCCATGACCACATGCAGCTTGATCTTTCAACCCATGACGAGATGCAACTAGACCTCTCCACATTTGAAGTTAATCTGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

1117

Amino Acids

121.84

Weight (kDa)

5.09

Isoelectric Point (pI)

39.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 455 - 537 4.5e-17 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1865
Acc36I ACCTGC 3 cut(s) 631, 700, 2341
AccB1I GGYRCC 1 cut(s) 2129
AccB7I CCANNNNNTGG 2 cut(s) 460, 934
AccI GTMKAC 2 cut(s) 2303, 2562
AccII CGCG 2 cut(s) 171, 557
AccIII TCCGGA 1 cut(s) 2812
AciI CCGC 8 cut(s) 135, 169, 212, 218, 278, 557, 2450, 2748
AclWI GGATC 4 cut(s) 738, 1080, 2353, 3156
AcoI YGGCCR 3 cut(s) 1405, 2205, 2530
AcsI RAATTY 8 cut(s) 436, 739, 1918, 2365, 2515, 2593, 2624, 3035
AcuI CTGAAG 8 cut(s) 456, 603, 1110, 1188, 2483, 2877, 2957, 3030
AcyI GRCGYC 1 cut(s) 1862
AdeI CACNNNGTG 1 cut(s) 662
AfaI GTAC 6 cut(s) 130, 690, 1149, 1348, 2408, 2485
AfeI AGCGCT 1 cut(s) 2924
AfiI CCNNNNNNNGG 7 cut(s) 460, 934, 1879, 2029, 2187, 2368, 3151
AflIII ACRYGT 2 cut(s) 1621, 1964
AjiI CACGTC 1 cut(s) 1622
AjnI CCWGG 6 cut(s) 841, 1402, 1548, 1969, 2774, 3144
AjuI GAANNNNNNNTTGG 4 cut(s) 1486, 1518, 2528, 2560
Alw21I GWGCWC 3 cut(s) 1568, 2885, 3142
Alw26I GTCTC 3 cut(s) 1363, 2179, 2609
Alw44I GTGCAC 2 cut(s) 1564, 3138
AlwI GGATC 4 cut(s) 738, 1080, 2353, 3156
Ama87I CYCGRG 1 cut(s) 939
Aor13HI TCCGGA 1 cut(s) 2812
Aor51HI AGCGCT 1 cut(s) 2924
AoxI GGCC 8 cut(s) 166, 936, 1400, 1405, 2020, 2205, 2530, 2765
ApaLI GTGCAC 2 cut(s) 1564, 3138
ApoI RAATTY 8 cut(s) 436, 739, 1918, 2365, 2515, 2593, 2624, 3035
ArsI GACNNNNNNTTYG 2 cut(s) 1481, 1513
Asp700I GAANNNNTTC 1 cut(s) 622
AspLEI GCGC 2 cut(s) 557, 2925
AspS9I GGNCC 6 cut(s) 166, 925, 937, 1489, 2021, 2930
AsuC2I CCSGG 3 cut(s) 940, 941, 2024
AsuHPI GGTGA 8 cut(s) 167, 554, 1016, 1985, 2226, 2276, 2601, 2890
AvaI CYCGRG 1 cut(s) 939
AvaII GGWCC 3 cut(s) 925, 1489, 2930
BaeGI GKGCMC 2 cut(s) 1568, 3142
BaeI ACNNNNGTAYC 2 cut(s) 2696, 2729
BalI TGGCCA 3 cut(s) 1407, 2207, 2532
BanI GGYRCC 1 cut(s) 2129
BanII GRGCYC 2 cut(s) 2553, 2885
BauI CACGAG 1 cut(s) 441
BbsI GAAGAC 1 cut(s) 2021
Bbv12I GWGCWC 3 cut(s) 1568, 2885, 3142
BbvCI CCTCAGC 1 cut(s) 51
BceAI ACGGC 2 cut(s) 974, 2958
BcgI CGANNNNNNTGC 6 cut(s) 402, 436, 2315, 2349, 2825, 2859
BciT130I CCWGG 6 cut(s) 843, 1404, 1550, 1971, 2776, 3146
BciVI GTATCC 1 cut(s) 2282
BclI TGATCA 2 cut(s) 997, 3181
BcnI CCSGG 3 cut(s) 940, 941, 2024
BcoDI GTCTC 3 cut(s) 1363, 2179, 2609
BfmI CTRYAG 1 cut(s) 2307
BfoI RGCGCY 1 cut(s) 2926
BfuAI ACCTGC 3 cut(s) 631, 700, 2341
BfuI GTATCC 1 cut(s) 2282
BmcAI AGTACT 2 cut(s) 2408, 2485
Bme1390I CCNGG 9 cut(s) 843, 940, 941, 1404, 1550, 1971, 2024, 2776, 3146
Bme18I GGWCC 3 cut(s) 925, 1489, 2930
BmeT110I CYCGRG 1 cut(s) 939
BmgBI CACGTC 1 cut(s) 1622
BmgT120I GGNCC 6 cut(s) 166, 925, 937, 1489, 2021, 2930
BmiI GGNNCC 3 cut(s) 926, 1490, 2131
BmrFI CCNGG 9 cut(s) 843, 940, 941, 1404, 1550, 1971, 2024, 2776, 3146
BpiI GAAGAC 1 cut(s) 2021
BplI GAGNNNNNCTC 4 cut(s) 717, 749, 2867, 2899
BpmI CTGGAG 1 cut(s) 341
Bpu10I CCTNAGC 2 cut(s) 51, 801
BpuEI CTTGAG 3 cut(s) 2024, 2500, 2775
BpuMI CCSGG 3 cut(s) 940, 941, 2024
BsaBI GATNNNNATC 1 cut(s) 996
BsaHI GRCGYC 1 cut(s) 1862
BsaJI CCNNGG 7 cut(s) 939, 1394, 1548, 1848, 2693, 2728, 3144
BsaWI WCCGGW 1 cut(s) 2812
BsaXI ACNNNNNCTCC 2 cut(s) 558, 588
Bsc4I CCNNNNNNNGG 7 cut(s) 460, 934, 1879, 2029, 2187, 2368, 3151
Bse1I ACTGG 2 cut(s) 2023, 3132
Bse3DI GCAATG 1 cut(s) 1842
Bse8I GATNNNNATC 1 cut(s) 996
BseAI TCCGGA 1 cut(s) 2812
BseBI CCWGG 6 cut(s) 843, 1404, 1550, 1971, 2776, 3146
BseDI CCNNGG 7 cut(s) 939, 1394, 1548, 1848, 2693, 2728, 3144
BseJI GATNNNNATC 1 cut(s) 996
BseLI CCNNNNNNNGG 7 cut(s) 460, 934, 1879, 2029, 2187, 2368, 3151
BseMI GCAATG 1 cut(s) 1842
BseNI ACTGG 2 cut(s) 2023, 3132
BseRI GAGGAG 6 cut(s) 153, 156, 173, 188, 239, 2400
BseSI GKGCMC 2 cut(s) 1568, 3142
BseYI CCCAGC 4 cut(s) 35, 1700, 2848, 3029
BsgI GTGCAG 2 cut(s) 678, 2360
Bsh1236I CGCG 2 cut(s) 171, 557
BshFI GGCC 8 cut(s) 168, 938, 1402, 1407, 2022, 2207, 2532, 2767
BshNI GGYRCC 1 cut(s) 2129
BsiHKAI GWGCWC 3 cut(s) 1568, 2885, 3142
BsiHKCI CYCGRG 1 cut(s) 939
BsiSI CCGG 4 cut(s) 719, 940, 2024, 2813
BslFI GGGAC 2 cut(s) 483, 1502
BslI CCNNNNNNNGG 7 cut(s) 460, 934, 1879, 2029, 2187, 2368, 3151
BsmAI GTCTC 3 cut(s) 1363, 2179, 2609
BsmBI CGTCTC 1 cut(s) 2179
BsmFI GGGAC 2 cut(s) 483, 1502
BsmI GAATGC 1 cut(s) 2848
BsnI GGCC 8 cut(s) 168, 938, 1402, 1407, 2022, 2207, 2532, 2767
BsoBI CYCGRG 1 cut(s) 939
Bsp1286I GDGCHC 4 cut(s) 1568, 2553, 2885, 3142
Bsp13I TCCGGA 1 cut(s) 2812
Bsp1407I TGTACA 1 cut(s) 1147
Bsp19I CCATGG 1 cut(s) 1394
BspACI CCGC 8 cut(s) 135, 169, 212, 218, 278, 557, 2450, 2748
BspANI GGCC 8 cut(s) 168, 938, 1402, 1407, 2022, 2207, 2532, 2767
BspEI TCCGGA 1 cut(s) 2812
BspFNI CGCG 2 cut(s) 171, 557
BspHI TCATGA 2 cut(s) 790, 3184
BspLI GGNNCC 3 cut(s) 926, 1490, 2131
BspMI ACCTGC 3 cut(s) 631, 700, 2341
BspPI GGATC 4 cut(s) 738, 1080, 2353, 3156
BspQI GCTCTTC 2 cut(s) 1379, 2056
BspT107I GGYRCC 1 cut(s) 2129
BsrDI GCAATG 1 cut(s) 1842
BsrGI TGTACA 1 cut(s) 1147
BsrI ACTGG 2 cut(s) 2023, 3132
BssECI CCNNGG 7 cut(s) 939, 1394, 1548, 1848, 2693, 2728, 3144
BssNI GRCGYC 1 cut(s) 1862
BssSI CACGAG 1 cut(s) 441
BssT1I CCWWGG 3 cut(s) 1394, 1848, 2728
Bst2BI CACGAG 1 cut(s) 441
Bst2UI CCWGG 6 cut(s) 843, 1404, 1550, 1971, 2776, 3146
Bst4CI ACNGT 8 cut(s) 430, 574, 703, 1267, 1466, 1806, 2295, 3061
Bst6I CTCTTC 5 cut(s) 355, 1251, 1379, 2056, 2676
BstACI GRCGYC 1 cut(s) 1862
BstAUI TGTACA 1 cut(s) 1147
BstC8I GCNNGC 7 cut(s) 828, 1051, 1989, 2287, 2748, 2765, 3019
BstDSI CCRYGG 2 cut(s) 1394, 2693
BstEII GGTNACC 1 cut(s) 2607
BstFNI CGCG 2 cut(s) 171, 557
BstH2I RGCGCY 1 cut(s) 2926
BstHHI GCGC 2 cut(s) 557, 2925
BstMAI GTCTC 3 cut(s) 1363, 2179, 2609
BstMWI GCNNNNNNNGC 8 cut(s) 248, 257, 266, 275, 284, 410, 2204, 3014
BstNI CCWGG 6 cut(s) 843, 1404, 1550, 1971, 2776, 3146
BstNSI RCATGY 5 cut(s) 1053, 1968, 1991, 2116, 3283
BstPI GGTNACC 1 cut(s) 2607
BstSCI CCNGG 9 cut(s) 841, 938, 939, 1402, 1548, 1969, 2022, 2774, 3144
BstSFI CTRYAG 1 cut(s) 2307
BstSLI GKGCMC 2 cut(s) 1568, 3142
BstUI CGCG 2 cut(s) 171, 557
BstV2I GAAGAC 1 cut(s) 2021
BstX2I RGATCY 2 cut(s) 730, 2345
BstXI CCANNNNNNTGG 3 cut(s) 1913, 2540, 2675
BstYI RGATCY 2 cut(s) 730, 2345
BsuI GTATCC 1 cut(s) 2282
BsuRI GGCC 8 cut(s) 168, 938, 1402, 1407, 2022, 2207, 2532, 2767
BtgI CCRYGG 2 cut(s) 1394, 2693
BtgZI GCGATG 1 cut(s) 1457
BtrI CACGTC 1 cut(s) 1622
BtsI GCAGTG 2 cut(s) 1568, 1717
BtsIMutI CAGTG 5 cut(s) 435, 1116, 1568, 1717, 3139
BveI ACCTGC 3 cut(s) 631, 700, 2341
Cac8I GCNNGC 7 cut(s) 828, 1051, 1989, 2287, 2748, 2765, 3019
CciI TCATGA 2 cut(s) 790, 3184
CfoI GCGC 2 cut(s) 557, 2925
Cfr13I GGNCC 6 cut(s) 166, 925, 937, 1489, 2021, 2930
Cfr9I CCCGGG 1 cut(s) 939
Csp6I GTAC 6 cut(s) 129, 689, 1148, 1347, 2407, 2484
CspCI CAANNNNNGTGG 4 cut(s) 1105, 1140, 2554, 2589
CviQI GTAC 6 cut(s) 129, 689, 1148, 1347, 2407, 2484
DraI TTTAAA 2 cut(s) 1101, 1524
DraIII CACNNNGTG 1 cut(s) 662
EaeI YGGCCR 3 cut(s) 1405, 2205, 2530
Eam1104I CTCTTC 5 cut(s) 355, 1251, 1379, 2056, 2676
EarI CTCTTC 5 cut(s) 355, 1251, 1379, 2056, 2676
EciI GGCGGA 1 cut(s) 227
Ecl136II GAGCTC 1 cut(s) 2883
Eco130I CCWWGG 3 cut(s) 1394, 1848, 2728
Eco24I GRGCYC 2 cut(s) 2553, 2885
Eco32I GATATC 1 cut(s) 907
Eco47I GGWCC 3 cut(s) 925, 1489, 2930
Eco47III AGCGCT 1 cut(s) 2924
Eco53kI GAGCTC 1 cut(s) 2883
Eco57I CTGAAG 8 cut(s) 456, 603, 1110, 1188, 2483, 2877, 2957, 3030
Eco88I CYCGRG 1 cut(s) 939
Eco91I GGTNACC 1 cut(s) 2607
EcoICRI GAGCTC 1 cut(s) 2883
EcoO65I GGTNACC 1 cut(s) 2607
EcoRI GAATTC 3 cut(s) 436, 739, 2365
EcoRII CCWGG 6 cut(s) 841, 1402, 1548, 1969, 2774, 3144
EcoRV GATATC 1 cut(s) 907
EcoT14I CCWWGG 3 cut(s) 1394, 1848, 2728
EcoT22I ATGCAT 3 cut(s) 1055, 1652, 3195
EcoT38I GRGCYC 2 cut(s) 2553, 2885
ErhI CCWWGG 3 cut(s) 1394, 1848, 2728
Esp3I CGTCTC 1 cut(s) 2179
FalI AAGNNNNNCTT 4 cut(s) 849, 881, 1841, 1873
FaqI GGGAC 2 cut(s) 483, 1502
FauI CCCGC 1 cut(s) 2755
FauNDI CATATG 2 cut(s) 1233, 1786
FbaI TGATCA 2 cut(s) 997, 3181
FblI GTMKAC 2 cut(s) 2303, 2562
FriOI GRGCYC 2 cut(s) 2553, 2885
GlaI GCGC 2 cut(s) 556, 2924
GsaI CCCAGC 4 cut(s) 39, 1704, 2852, 3033
GsuI CTGGAG 1 cut(s) 341
HaeII RGCGCY 1 cut(s) 2926
HaeIII GGCC 8 cut(s) 168, 938, 1402, 1407, 2022, 2207, 2532, 2767
HapII CCGG 4 cut(s) 719, 940, 2024, 2813
HhaI GCGC 2 cut(s) 557, 2925
Hin1I GRCGYC 1 cut(s) 1862
Hin6I GCGC 2 cut(s) 555, 2923
HinP1I GCGC 2 cut(s) 555, 2923
HincII GTYRAC 1 cut(s) 813
HindII GTYRAC 1 cut(s) 813
HindIII AAGCTT 2 cut(s) 468, 1794
HpaII CCGG 4 cut(s) 719, 940, 2024, 2813
HphI GGTGA 8 cut(s) 167, 554, 1016, 1985, 2226, 2276, 2601, 2890
Hpy166II GTNNAC 6 cut(s) 813, 976, 1566, 2304, 2563, 3140
Hpy8I GTNNAC 6 cut(s) 813, 976, 1566, 2304, 2563, 3140
HpyCH4III ACNGT 8 cut(s) 430, 574, 703, 1267, 1466, 1806, 2295, 3061
HpyCH4IV ACGT 4 cut(s) 1621, 1862, 2062, 3116
HpyF10VI GCNNNNNNNGC 8 cut(s) 248, 257, 266, 275, 284, 410, 2204, 3014
HpySE526I ACGT 4 cut(s) 1621, 1862, 2062, 3116
Hsp92I GRCGYC 1 cut(s) 1862
HspAI GCGC 2 cut(s) 555, 2923
Kpn2I TCCGGA 1 cut(s) 2812
Ksp22I TGATCA 2 cut(s) 997, 3181
LguI GCTCTTC 2 cut(s) 1379, 2056
LmnI GCTCC 3 cut(s) 239, 2084, 2695
MaeII ACGT 4 cut(s) 1621, 1862, 2062, 3116
MaeIII GTNAC 8 cut(s) 574, 654, 985, 1111, 1162, 2430, 2607, 3065
MfeI CAATTG 1 cut(s) 1594
MflI RGATCY 2 cut(s) 730, 2345
MhlI GDGCHC 4 cut(s) 1568, 2553, 2885, 3142
MlsI TGGCCA 3 cut(s) 1407, 2207, 2532
MluNI TGGCCA 3 cut(s) 1407, 2207, 2532
MlyI GAGTC 5 cut(s) 185, 505, 513, 662, 2806
MmeI TCCRAC 1 cut(s) 1905
Mox20I TGGCCA 3 cut(s) 1407, 2207, 2532
Mph1103I ATGCAT 3 cut(s) 1055, 1652, 3195
MroI TCCGGA 1 cut(s) 2812
MroXI GAANNNNTTC 1 cut(s) 622
MscI TGGCCA 3 cut(s) 1407, 2207, 2532
MseI TTAA 8 cut(s) 645, 950, 1100, 1523, 2123, 2256, 2591, 3339
MslI CAYNNNNRTG 5 cut(s) 678, 684, 1211, 1645, 3183
Msp20I TGGCCA 3 cut(s) 1407, 2207, 2532
MspA1I CMGCKG 3 cut(s) 251, 269, 2450
MspI CCGG 4 cut(s) 719, 940, 2024, 2813
MspR9I CCNGG 9 cut(s) 843, 940, 941, 1404, 1550, 1971, 2024, 2776, 3146
MssI GTTTAAAC 1 cut(s) 1101
MunI CAATTG 1 cut(s) 1594
Mva1269I GAATGC 1 cut(s) 2848
MvaI CCWGG 6 cut(s) 843, 1404, 1550, 1971, 2776, 3146
MvnI CGCG 2 cut(s) 171, 557
MwoI GCNNNNNNNGC 8 cut(s) 248, 257, 266, 275, 284, 410, 2204, 3014
NciI CCSGG 3 cut(s) 940, 941, 2024
NcoI CCATGG 1 cut(s) 1394
NdeI CATATG 2 cut(s) 1233, 1786
NlaIV GGNNCC 3 cut(s) 926, 1490, 2131
NmuCI GTSAC 5 cut(s) 574, 654, 1111, 2430, 2607
NsiI ATGCAT 3 cut(s) 1055, 1652, 3195
NspI RCATGY 5 cut(s) 1053, 1968, 1991, 2116, 3283
PaeI GCATGC 2 cut(s) 1053, 1991
PagI TCATGA 2 cut(s) 790, 3184
PciI ACATGT 1 cut(s) 1964
PciSI GCTCTTC 2 cut(s) 1379, 2056
PctI GAATGC 1 cut(s) 2848
PdmI GAANNNNTTC 1 cut(s) 622
PfeI GAWTC 5 cut(s) 610, 1680, 1715, 1742, 1766
PflFI GACNNNGTC 1 cut(s) 2567
PflMI CCANNNNNTGG 2 cut(s) 460, 934
PfoI TCCNGGA 1 cut(s) 841
PleI GAGTC 5 cut(s) 184, 504, 512, 661, 2805
PmeI GTTTAAAC 1 cut(s) 1101
PpsI GAGTC 5 cut(s) 184, 504, 512, 661, 2805
PscI ACATGT 1 cut(s) 1964
Psp124BI GAGCTC 1 cut(s) 2885
Psp6I CCWGG 6 cut(s) 841, 1402, 1548, 1969, 2774, 3144
PspEI GGTNACC 1 cut(s) 2607
PspFI CCCAGC 4 cut(s) 35, 1700, 2848, 3029
PspGI CCWGG 6 cut(s) 841, 1402, 1548, 1969, 2774, 3144
PspN4I GGNNCC 3 cut(s) 926, 1490, 2131
PspPI GGNCC 6 cut(s) 166, 925, 937, 1489, 2021, 2930
PsuI RGATCY 2 cut(s) 730, 2345
PsyI GACNNNGTC 1 cut(s) 2567
PvuII CAGCTG 2 cut(s) 251, 269
RsaI GTAC 6 cut(s) 130, 690, 1149, 1348, 2408, 2485
RsaNI GTAC 6 cut(s) 129, 689, 1148, 1347, 2407, 2484
RseI CAYNNNNRTG 5 cut(s) 678, 684, 1211, 1645, 3183
SacI GAGCTC 1 cut(s) 2885
SapI GCTCTTC 2 cut(s) 1379, 2056
SaqAI TTAA 8 cut(s) 645, 950, 1100, 1523, 2123, 2256, 2591, 3339
Sau96I GGNCC 6 cut(s) 166, 925, 937, 1489, 2021, 2930
ScaI AGTACT 2 cut(s) 2408, 2485
SchI GAGTC 5 cut(s) 185, 505, 513, 662, 2806
ScrFI CCNGG 9 cut(s) 843, 940, 941, 1404, 1550, 1971, 2024, 2776, 3146
SduI GDGCHC 4 cut(s) 1568, 2553, 2885, 3142
SfcI CTRYAG 1 cut(s) 2307
SinI GGWCC 3 cut(s) 925, 1489, 2930
SmaI CCCGGG 1 cut(s) 941
SmiMI CAYNNNNRTG 5 cut(s) 678, 684, 1211, 1645, 3183
SmlI CTYRAG 3 cut(s) 2039, 2479, 2790
SmoI CTYRAG 3 cut(s) 2039, 2479, 2790
SphI GCATGC 2 cut(s) 1053, 1991
SsiI CCGC 8 cut(s) 135, 169, 212, 218, 278, 557, 2450, 2748
SstI GAGCTC 1 cut(s) 2885
StyD4I CCNGG 9 cut(s) 841, 938, 939, 1402, 1548, 1969, 2022, 2774, 3144
StyI CCWWGG 3 cut(s) 1394, 1848, 2728
TaaI ACNGT 8 cut(s) 430, 574, 703, 1267, 1466, 1806, 2295, 3061
TaiI ACGT 4 cut(s) 1624, 1865, 2065, 3119
TaqI TCGA 6 cut(s) 494, 651, 1273, 1718, 2154, 2835
TaqII GACCGA 1 cut(s) 1506
TatI WGTACW 4 cut(s) 128, 1147, 2406, 2483
TauI GCSGC 1 cut(s) 171
TfiI GAWTC 5 cut(s) 610, 1680, 1715, 1742, 1766
Tru1I TTAA 8 cut(s) 645, 950, 1100, 1523, 2123, 2256, 2591, 3339
Tru9I TTAA 8 cut(s) 645, 950, 1100, 1523, 2123, 2256, 2591, 3339
TscAI CASTG 5 cut(s) 435, 1123, 1568, 1717, 3139
TseFI GTSAC 5 cut(s) 574, 654, 1111, 2430, 2607
Tsp45I GTSAC 5 cut(s) 574, 654, 1111, 2430, 2607
TspGWI ACGGA 3 cut(s) 1862, 2203, 2710
TspMI CCCGGG 1 cut(s) 939
TspRI CASTG 5 cut(s) 435, 1123, 1568, 1717, 3139
Tth111I GACNNNGTC 1 cut(s) 2567
Van91I CCANNNNNTGG 2 cut(s) 460, 934
VneI GTGCAC 2 cut(s) 1564, 3138
VpaK11BI GGWCC 3 cut(s) 925, 1489, 2930
XapI RAATTY 8 cut(s) 436, 739, 1918, 2365, 2515, 2593, 2624, 3035
XbaI TCTAGA 1 cut(s) 2416
XceI RCATGY 5 cut(s) 1053, 1968, 1991, 2116, 3283
XmaI CCCGGG 1 cut(s) 939
XmiI GTMKAC 2 cut(s) 2303, 2562
XmnI GAANNNNTTC 1 cut(s) 622
ZraI GACGTC 1 cut(s) 1863
ZrmI AGTACT 2 cut(s) 2408, 2485
Zsp2I ATGCAT 3 cut(s) 1055, 1652, 3195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.