MD01G1112200.v1.1

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003478
Physical Location & Seq
Reverse (-)
22640568 .. 22646077
5510 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1112200.v1.1.491

Sequence Viewer

Length: 3624 bp
ATGGATTTTGCATTGGGTAGTGGGTCAGAGTCCACTGTGAGTGTGAGCGGACATGTGGCTGGTGAAACCCTAGCTGGGGCTGAGGCTTGTGTGAGTGAGGGTCAGGTTGAGGGGTCTTTTGGGGAGGATGTTGGTGACGATGAAGGCGGTGATTGTGAGGGGGATGATATAATGGTGGAGGTTCTGGGTTCTAATGTCTATGTTGGTGGAGTTTGCACTAATGGGGATGGGGGCGATGAGGGGGTGGGACGTGATGAGTCGGTTGAGGTTGATATGGGGTCGGAGGGAAATGTGAGATCTTCCGGAGGAGATGGTGGTAAAGTGGGTGAGCTGGATTCGGTGGCTGCAGACATTAGTTCAACAGTTATAGAAACCCAGGTTGTTCATTTTGAGGAAGCTGAGATGGTTGTGAGGCAGGATGTGGATTCAGAGGAAGTTAATGTTTCGGATGAAAAAGCACAGGATGCCACAGCAGAAAATGGACTTGGGGGTTCCTTGATTGAACCTGTAGGTAGTGAAACACAAGTTGTCGTCAGTGAAGTAGTGAGGGAAAGTGTAGAAGTTTCCGCGGGTGGATTGGTGGAGGTTGTGGAACATGAAAAGAAGTTGGTTGTTGGTGAGGTTGATGCACTCAATGATTTGAAGGCACAAAAAGCTGGTGTTTCAGATGATGAAGTATGGAATCCTGGAATTGAAAAAGCTACAGTGACTGTTAATGAAGAAGGTAATCCCAAACCCTCAAGTGAACAAAGCCAAGTTTCTGCTGGTGGTGATGATGTTGCCGGGGTGGACAATGTAGATATTCTGACTTCCAAAGTTGCAGGTAAGGAAACTGGTAAAACTAATGAAAATTCGAGTAATCCAGTAGAAGAACAGCTGGTTAAGATTGAGCCAGTAGGTGGTAGCACCCATAGTGGCAGCAATGTACCTGAAAATTCAGTGTCTTCTCCTATGCCGCCTCAAGTAGTTCTTGGAAGTGACACGGGAGCAACAGGTGCACAAGATTCGGAAAAAAGCCAGTTCTTGAAACCTGAAGAGAGTGTAGAGGACATGGTTCATGATATTGCTTTGGTTTCAACTACCTTATCTCTGCCTGCTCAAGTAATTCCTGGAGGTGTAGTTTCAGTAACAGATGGAAGGGGCCTTTTGAATTCTGAAAAAGAACTGCACATGAAGCCTGAAGAGAGTATAGGCAAGAACACGTTCCATGATACTGTGCTGGTTGAATCAAATACAGAGCAAGAGATGGAAAAGGACAGCCACGGTAATGGTGCTGAACAGGTTGGCGTGGATGGAGCAGATCAATCCCTGGAGTCTGAAAAGTGTTTAGAAAAGTCTGCGACTGCTTATGTTGCTCCAATTCATCCCGATATGGAAGCAGAGGAAGTCAATGAACAAGTGAGTGATGCTGAGGGTGTATTAAATGGAGGGGAACAGATAGAAGATGAAAGGGATATTACTCCCATGGACACTGAGGAGGTTTTGGATTCTGTGACTGCAGCTGAGACCAATGTTGTGCATGAGGTAGGAGTTAAAGAACAATTTACTGATGCTGTGCTAGATGGTTTACACGGAGGGCAATACATGGAAGGTGAAAAAGAAGCCACTGATGCTGAACAACCAAAAGCGGGTGAAGAGGACATTCTTAAACAAGAAGCTATCGAAGGAAGCTCAGAAATTGTGCCTCAACCGAGATATGAGCTGCCACCAGAAAATCATGTGTTCTCTGCATCTGATTTAGTTTGGGGTAAAGTGAAGAGCCATCCATGGTGGCCTGGACAGATATTTGATTATACAGTTGCATCTGAGAAGGCAATGAAATATCACAAAAAAGATTGCTTTTTGGTAGCATATTTTGGGGACCGTACGTTTGCTTGGAATGAACCATCTAGTTTAAGGCCTTTTCGATCTTACTTTTCCCAGTCAGAAAAGCAAGGTAATTCAGAAGCGTTCCAGAATGCTGTCAATTGTGCACTGGAAGAAGTTTCGAGACGTGTTGGATTGGGGCTAGCATGCTCTTGCATACCAAAAGAAGTCTATGAGAAGATGAGGTTCCAGAAAGTTGAAAATGCTGGGATCTGCCAAGAATCCAGTAGAAGAGATGAAGTGGATGAATCGGCAAGTGCCAGTTCTCTTGAATGTAATAAATTACTGGAATACATAAAAGCTTTATCACGGTTTCCGTCTGGTAACAGTGACCGATTAGAAGTTGTGATAGCTAAGGCTCATATGTTAGCATTTTATCGCTTGAAGGGTTACTACAACTTGCCAAAATTCCAATCTTGTGGAGATATATTGGAAAACAAAGATGAAAGGATTGAGCATACAACTCCTAATGGTAAGGATGTTAAAGGTACTTTCTCTGGCCCCGACATTATTACGTATTCTCACAAGCGTAAACATAATTTAAGAGGTGGCGTGTATTCTAAGGTAAAAGAAAGAAGCCTGTCGGAATTAATGGACGGCGGCCTAGATTCTCTAGACGGCGGTGATTGGTTGGATGGGAACGAAACTGATGGTTTGGTCTCACCATCCTCTGGCAAGAGACGGAAGGGTTTTGAGTATCATGTTGATGACGATGGAAGGAAAGTTACCTCCAACAGTGACAGACCTGCTGGGGATGGATCTGCTGTTGCCTTCCAGAGTTCTGGTGATGTCCATAGAGGGAAAACAATTGATGGAACAGAGTACTCATCGCTAGATGAATTACTGTCACAACTTCAGTCGGCAGCAGAAGATCCGCGGAAGGATTACCATAGCTTGAAAACTGTTGTTAATTTCTTCTGTGATTTTAGGAATTCGGTAGCTGTAGGTCAGCTTCCTGGGATGGAGCATGTAGCTGTGGACAAAGTTGGTGGTAGAAAGAGGAAGTCAAATTCTGCATTTGGGTTGCCGGAAACTTTTGAATTTGATGATATGAATGATACTTATTGGACAGACAGGGTAATCCAAAATGGCGCTGAAGAGCAAACACCTCGAAGGGGCAGGAAGGTTAATTATCAACCTGTTGTTCTTGCCCAACCAGAAAAATCCCCTCAAGAGGGTCGCAGGCCATACACCAGGAAGCGGTATTCCCAAACCGTTAATGTTTTGCCGCCAGAGAAACCTGTTGGCTATGTGGATGAGAATGCCCCAACAGAACTTGTTATGAACTTCACTGAGGTGAATTCTGTTCCCTCAGAAATAAAGCTAAATAGAATGTTTAGGCGCTTTGGACCTCTGAAGGAAGCTGAAACAGAAGTTGATAGGGAGAGCAGTCGTGCTAGAGTTGTTTTCAAGAAATGTTCTGATGCAGAGGTAGCTATTGATAGTGCGGGAAAATTCAAAATCTTCGGATCAATACCTGTAAACTACCAGCTCAATTATACTCCATCTCAGCTCAACTATACTCCGTCTATCCAATTTAGCATTTCTCCCAGTGCCACAACCCATGATCAGGAGATGCAACTTGTTCTTTCCTCCCATGATCAAGAGATGCACCTTGATCTCTCCGCCCACGACCAAATGCAACTTGATCTTTCCACCCACGACCATATGCAACTGGATATGCAACTTGATCTTTCCTCCCATGACCAGATGCACCTTGATCTCTCCACCTTCGATAACCTGGAGAGTAAATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

1208

Amino Acids

131.21

Weight (kDa)

4.54

Isoelectric Point (pI)

42.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 579 - 662 7.1e-18 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 812, 2620
AccB7I CCANNNNNTGG 2 cut(s) 899, 2537
AccBSI CCGCTC 1 cut(s) 48
AccII CGCG 2 cut(s) 569, 2743
AccIII TCCGGA 1 cut(s) 302
AclWI GGATC 4 cut(s) 2084, 2632, 2732, 3343
AcsI RAATTY 9 cut(s) 850, 934, 1150, 2273, 2797, 2875, 2906, 3166, 3320
AcuI CTGAAG 5 cut(s) 1053, 1200, 2705, 2982, 3242
AfaI GTAC 4 cut(s) 927, 1867, 2356, 2690
AflIII ACRYGT 3 cut(s) 52, 1200, 1993
AjiI CACGTC 2 cut(s) 251, 1994
AjnI CCWGG 8 cut(s) 375, 685, 1108, 1308, 1774, 2821, 3059, 3606
AjuI GAANNNNNNNTTGG 2 cut(s) 468, 500
AleI CACNNNNGTG 1 cut(s) 3161
Alw21I GWGCWC 2 cut(s) 1000, 1975
Alw26I GTCTC 4 cut(s) 1499, 1984, 2530, 2539
Alw44I GTGCAC 2 cut(s) 996, 1971
AlwI GGATC 4 cut(s) 2084, 2632, 2732, 3343
AlwNI CAGNNNCTG 1 cut(s) 710
Aor13HI TCCGGA 1 cut(s) 302
AoxI GGCC 6 cut(s) 1141, 1772, 1898, 2365, 2467, 3050
ApaLI GTGCAC 2 cut(s) 996, 1971
ApeKI GCWGC 5 cut(s) 344, 918, 1499, 1702, 2729
ApoI RAATTY 9 cut(s) 850, 934, 1150, 2273, 2797, 2875, 2906, 3166, 3320
ArsI GACNNNNNNTTYG 2 cut(s) 1853, 1885
AseI ATTAAT 1 cut(s) 2456
Asp700I GAANNNNTTC 1 cut(s) 1202
AspLEI GCGC 2 cut(s) 2960, 3210
AspS9I GGNCC 4 cut(s) 1141, 1861, 2366, 3215
AsuC2I CCSGG 1 cut(s) 784
AsuNHI GCTAGC 1 cut(s) 2008
AvaII GGWCC 2 cut(s) 1861, 3215
BaeGI GKGCMC 2 cut(s) 1000, 1975
BarI GAAGNNNNNNTAC 6 cut(s) 2243, 2275, 2575, 2607, 2802, 2834
BbsI GAAGAC 1 cut(s) 936
Bbv12I GWGCWC 2 cut(s) 1000, 1975
BbvCI CCTCAGC 2 cut(s) 81, 1410
BbvI GCAGC 5 cut(s) 331, 930, 1511, 1689, 2741
BceAI ACGGC 2 cut(s) 2479, 2500
BcgI CGANNNNNNTGC 4 cut(s) 987, 1021, 2957, 2991
BciT130I CCWGG 8 cut(s) 377, 687, 1110, 1310, 1776, 2823, 3061, 3608
BclI TGATCA 2 cut(s) 3433, 3466
BcnI CCSGG 1 cut(s) 784
BcoDI GTCTC 4 cut(s) 1499, 1984, 2530, 2539
BfaI CTAG 8 cut(s) 71, 1559, 1890, 2009, 2471, 2480, 2699, 3264
BfmI CTRYAG 5 cut(s) 345, 507, 702, 1497, 2808
BfoI RGCGCY 2 cut(s) 2961, 3211
BfuAI ACCTGC 2 cut(s) 812, 2620
BglII AGATCT 1 cut(s) 296
BisI GCNGC 8 cut(s) 345, 919, 956, 1500, 1703, 2467, 2730, 3095
BlsI GCNGC 8 cut(s) 346, 920, 957, 1501, 1704, 2468, 2731, 3096
BmcAI AGTACT 1 cut(s) 2690
Bme1390I CCNGG 9 cut(s) 377, 687, 784, 1110, 1310, 1776, 2823, 3061, 3608
Bme18I GGWCC 2 cut(s) 1861, 3215
BmgBI CACGTC 2 cut(s) 251, 1994
BmgT120I GGNCC 4 cut(s) 1141, 1861, 2366, 3215
BmiI GGNNCC 5 cut(s) 493, 1142, 1862, 2054, 2368
BmrFI CCNGG 9 cut(s) 377, 687, 784, 1110, 1310, 1776, 2823, 3061, 3608
BmrI ACTGGG 2 cut(s) 1915, 3411
BmtI GCTAGC 1 cut(s) 2012
BmuI ACTGGG 2 cut(s) 1915, 3411
BpiI GAAGAC 1 cut(s) 936
BpmI CTGGAG 2 cut(s) 1131, 1331
Bpu10I CCTNAGC 3 cut(s) 81, 1410, 2220
BpuEI CTTGAG 4 cut(s) 724, 945, 1083, 3021
BpuMI CCSGG 1 cut(s) 784
BsaAI YACGTR 1 cut(s) 2382
BsaI GGTCTC 2 cut(s) 1499, 2530
BsaJI CCNNGG 9 cut(s) 375, 567, 783, 1261, 1308, 1464, 1766, 2741, 2822
BsaWI WCCGGW 1 cut(s) 302
BsaXI ACNNNNNCTCC 2 cut(s) 1104, 1134
Bse3DI GCAATG 2 cut(s) 928, 1821
BseAI TCCGGA 1 cut(s) 302
BseBI CCWGG 8 cut(s) 377, 687, 1110, 1310, 1776, 2823, 3061, 3608
BseDI CCNNGG 9 cut(s) 375, 567, 783, 1261, 1308, 1464, 1766, 2741, 2822
BseMI GCAATG 2 cut(s) 928, 1821
BseRI GAGGAG 2 cut(s) 321, 1490
BseSI GKGCMC 2 cut(s) 1000, 1975
BseXI GCAGC 5 cut(s) 331, 930, 1511, 1689, 2741
BseYI CCCAGC 3 cut(s) 74, 2072, 2615
BsgI GTGCAG 1 cut(s) 1151
Bsh1236I CGCG 2 cut(s) 569, 2743
BshFI GGCC 6 cut(s) 1143, 1774, 1900, 2367, 2469, 3052
BsiHKAI GWGCWC 2 cut(s) 1000, 1975
BsiSI CCGG 3 cut(s) 303, 783, 2894
BsiWI CGTACG 1 cut(s) 1865
BslFI GGGAC 2 cut(s) 261, 1874
BsmAI GTCTC 4 cut(s) 1499, 1984, 2530, 2539
BsmBI CGTCTC 2 cut(s) 1984, 2539
BsmFI GGGAC 2 cut(s) 261, 1874
BsmI GAATGC 2 cut(s) 1963, 3133
BsnI GGCC 6 cut(s) 1143, 1774, 1900, 2367, 2469, 3052
Bso31I GGTCTC 2 cut(s) 1499, 2530
Bsp1286I GDGCHC 2 cut(s) 1000, 1975
Bsp13I TCCGGA 1 cut(s) 302
Bsp19I CCATGG 2 cut(s) 1464, 1766
BspANI GGCC 6 cut(s) 1143, 1774, 1900, 2367, 2469, 3052
BspEI TCCGGA 1 cut(s) 302
BspFNI CGCG 2 cut(s) 569, 2743
BspHI TCATGA 1 cut(s) 1057
BspLI GGNNCC 5 cut(s) 493, 1142, 1862, 2054, 2368
BspMAI CTGCAG 2 cut(s) 349, 1501
BspMI ACCTGC 2 cut(s) 812, 2620
BspOI GCTAGC 1 cut(s) 2012
BspPI GGATC 4 cut(s) 2084, 2632, 2732, 3343
BspQI GCTCTTC 2 cut(s) 1751, 2958
BspTNI GGTCTC 2 cut(s) 1499, 2530
BsrBI CCGCTC 1 cut(s) 48
BsrDI GCAATG 2 cut(s) 928, 1821
BssECI CCNNGG 9 cut(s) 375, 567, 783, 1261, 1308, 1464, 1766, 2741, 2822
BssT1I CCWWGG 2 cut(s) 1464, 1766
Bst2UI CCWGG 8 cut(s) 377, 687, 1110, 1310, 1776, 2823, 3061, 3608
Bst6I CTCTTC 6 cut(s) 1029, 1176, 1629, 1751, 2092, 2958
BstAPI GCANNNNNTGC 2 cut(s) 464, 995
BstBAI YACGTR 1 cut(s) 2382
BstC8I GCNNGC 4 cut(s) 1095, 2010, 2014, 3050
BstDSI CCRYGG 5 cut(s) 567, 1261, 1464, 1766, 2741
BstFNI CGCG 2 cut(s) 569, 2743
BstH2I RGCGCY 2 cut(s) 2961, 3211
BstHHI GCGC 2 cut(s) 2960, 3210
BstMAI GTCTC 4 cut(s) 1499, 1984, 2530, 2539
BstMWI GCNNNNNNNGC 7 cut(s) 464, 653, 995, 1174, 1352, 1610, 3299
BstNI CCWGG 8 cut(s) 377, 687, 1110, 1310, 1776, 2823, 3061, 3608
BstNSI RCATGY 3 cut(s) 56, 2016, 2837
BstSCI CCNGG 9 cut(s) 375, 685, 782, 1108, 1308, 1774, 2821, 3059, 3606
BstSFI CTRYAG 5 cut(s) 345, 507, 702, 1497, 2808
BstSLI GKGCMC 2 cut(s) 1000, 1975
BstSNI TACGTA 1 cut(s) 2382
BstUI CGCG 2 cut(s) 569, 2743
BstV1I GCAGC 5 cut(s) 331, 930, 1511, 1689, 2741
BstV2I GAAGAC 1 cut(s) 936
BstX2I RGATCY 4 cut(s) 296, 2076, 2624, 2737
BstXI CCANNNNNNTGG 3 cut(s) 1268, 2285, 2648
BstYI RGATCY 4 cut(s) 296, 2076, 2624, 2737
BsuRI GGCC 6 cut(s) 1143, 1774, 1900, 2367, 2469, 3052
BtgI CCRYGG 5 cut(s) 567, 1261, 1464, 1766, 2741
BtgZI GCGATG 2 cut(s) 249, 2679
BtrI CACGTC 2 cut(s) 251, 1994
BveI ACCTGC 2 cut(s) 812, 2620
Cac8I GCNNGC 4 cut(s) 1095, 2010, 2014, 3050
CaiI CAGNNNCTG 1 cut(s) 710
CciI TCATGA 1 cut(s) 1057
CfoI GCGC 2 cut(s) 2960, 3210
Cfr13I GGNCC 4 cut(s) 1141, 1861, 2366, 3215
Cfr42I CCGCGG 2 cut(s) 570, 2744
Csp6I GTAC 4 cut(s) 926, 1866, 2355, 2689
CspCI CAANNNNNGTGG 2 cut(s) 2836, 2871
CviQI GTAC 4 cut(s) 926, 1866, 2355, 2689
Eam1104I CTCTTC 6 cut(s) 1029, 1176, 1629, 1751, 2092, 2958
EarI CTCTTC 6 cut(s) 1029, 1176, 1629, 1751, 2092, 2958
EciI GGCGGA 1 cut(s) 3481
Eco105I TACGTA 1 cut(s) 2382
Eco130I CCWWGG 2 cut(s) 1464, 1766
Eco147I AGGCCT 1 cut(s) 1900
Eco31I GGTCTC 2 cut(s) 1499, 2530
Eco47I GGWCC 2 cut(s) 1861, 3215
Eco57I CTGAAG 5 cut(s) 1053, 1200, 2705, 2982, 3242
EcoO109I RGGNCCY 1 cut(s) 1141
EcoRI GAATTC 3 cut(s) 1150, 2797, 3166
EcoRII CCWGG 8 cut(s) 375, 685, 1108, 1308, 1774, 2821, 3059, 3606
EcoT14I CCWWGG 2 cut(s) 1464, 1766
ErhI CCWWGG 2 cut(s) 1464, 1766
Esp3I CGTCTC 2 cut(s) 1984, 2539
FalI AAGNNNNNCTT 2 cut(s) 954, 986
FaqI GGGAC 2 cut(s) 261, 1874
FauI CCCGC 3 cut(s) 562, 1621, 3307
FauNDI CATATG 2 cut(s) 2229, 3534
FbaI TGATCA 2 cut(s) 3433, 3466
Fnu4HI GCNGC 8 cut(s) 345, 919, 956, 1500, 1703, 2467, 2730, 3095
Fsp4HI GCNGC 8 cut(s) 345, 919, 956, 1500, 1703, 2467, 2730, 3095
FspBI CTAG 8 cut(s) 71, 1559, 1890, 2009, 2471, 2480, 2699, 3264
GlaI GCGC 2 cut(s) 2959, 3209
GluI GCNGC 8 cut(s) 345, 919, 956, 1500, 1703, 2467, 2730, 3095
GsaI CCCAGC 3 cut(s) 78, 2076, 2619
GsuI CTGGAG 2 cut(s) 1131, 1331
HaeII RGCGCY 2 cut(s) 2961, 3211
HaeIII GGCC 6 cut(s) 1143, 1774, 1900, 2367, 2469, 3052
HapII CCGG 3 cut(s) 303, 783, 2894
HhaI GCGC 2 cut(s) 2960, 3210
Hin6I GCGC 2 cut(s) 2958, 3208
HinP1I GCGC 2 cut(s) 2958, 3208
HindIII AAGCTT 1 cut(s) 2166
HpaII CCGG 3 cut(s) 303, 783, 2894
Hpy166II GTNNAC 9 cut(s) 33, 746, 790, 998, 1568, 1973, 2399, 2845, 3349
Hpy8I GTNNAC 9 cut(s) 33, 746, 790, 998, 1568, 1973, 2399, 2845, 3349
HpyCH4IV ACGT 5 cut(s) 250, 1202, 1868, 1993, 2381
HpyF10VI GCNNNNNNNGC 7 cut(s) 464, 653, 995, 1174, 1352, 1610, 3299
HpySE526I ACGT 5 cut(s) 250, 1202, 1868, 1993, 2381
HspAI GCGC 2 cut(s) 2958, 3208
Kpn2I TCCGGA 1 cut(s) 302
Ksp22I TGATCA 2 cut(s) 3433, 3466
KspI CCGCGG 2 cut(s) 570, 2744
LguI GCTCTTC 2 cut(s) 1751, 2958
LmnI GCTCC 4 cut(s) 986, 1295, 1360, 2830
Lsp1109I GCAGC 5 cut(s) 331, 930, 1511, 1689, 2741
MaeI CTAG 8 cut(s) 71, 1559, 1890, 2009, 2471, 2480, 2699, 3264
MaeII ACGT 5 cut(s) 250, 1202, 1868, 1993, 2381
MbiI CCGCTC 1 cut(s) 48
MfeI CAATTG 2 cut(s) 1966, 2673
MflI RGATCY 4 cut(s) 296, 2076, 2624, 2737
MhlI GDGCHC 2 cut(s) 1000, 1975
MlyI GAGTC 3 cut(s) 38, 266, 1322
MmeI TCCRAC 5 cut(s) 261, 1978, 2430, 2478, 2622
MroI TCCGGA 1 cut(s) 302
MroXI GAANNNNTTC 1 cut(s) 1202
MslI CAYNNNNRTG 3 cut(s) 1266, 2571, 3161
MspA1I CMGCKG 4 cut(s) 569, 877, 1502, 2743
MspI CCGG 3 cut(s) 303, 783, 2894
MspR9I CCNGG 9 cut(s) 377, 687, 784, 1110, 1310, 1776, 2823, 3061, 3608
MunI CAATTG 2 cut(s) 1966, 2673
Mva1269I GAATGC 2 cut(s) 1963, 3133
MvaI CCWGG 8 cut(s) 377, 687, 1110, 1310, 1776, 2823, 3061, 3608
MvnI CGCG 2 cut(s) 569, 2743
MwoI GCNNNNNNNGC 7 cut(s) 464, 653, 995, 1174, 1352, 1610, 3299
NciI CCSGG 1 cut(s) 784
NcoI CCATGG 2 cut(s) 1464, 1766
NdeI CATATG 2 cut(s) 2229, 3534
NheI GCTAGC 1 cut(s) 2008
NlaIV GGNNCC 5 cut(s) 493, 1142, 1862, 2054, 2368
NmuCI GTSAC 7 cut(s) 134, 706, 977, 1492, 2195, 2603, 2712
NspI RCATGY 3 cut(s) 56, 2016, 2837
OliI CACNNNNGTG 1 cut(s) 3161
PaeI GCATGC 1 cut(s) 2016
PagI TCATGA 1 cut(s) 1057
PceI AGGCCT 1 cut(s) 1900
PciI ACATGT 1 cut(s) 52
PciSI GCTCTTC 2 cut(s) 1751, 2958
PctI GAATGC 2 cut(s) 1963, 3133
PdmI GAANNNNTTC 1 cut(s) 1202
PfeI GAWTC 9 cut(s) 335, 425, 682, 1004, 1226, 1487, 2087, 2114, 2474
Pfl23II CGTACG 1 cut(s) 1865
PflMI CCANNNNNTGG 2 cut(s) 899, 2537
PfoI TCCNGGA 2 cut(s) 685, 1108
PkrI GCNGC 8 cut(s) 346, 920, 957, 1501, 1704, 2468, 2731, 3096
PleI GAGTC 3 cut(s) 37, 265, 1321
PpsI GAGTC 3 cut(s) 37, 265, 1321
Ppu21I YACGTR 1 cut(s) 2382
PscI ACATGT 1 cut(s) 52
PshBI ATTAAT 1 cut(s) 2456
Psp6I CCWGG 8 cut(s) 375, 685, 1108, 1308, 1774, 2821, 3059, 3606
PspFI CCCAGC 3 cut(s) 74, 2072, 2615
PspGI CCWGG 8 cut(s) 375, 685, 1108, 1308, 1774, 2821, 3059, 3606
PspLI CGTACG 1 cut(s) 1865
PspN4I GGNNCC 5 cut(s) 493, 1142, 1862, 2054, 2368
PspPI GGNCC 4 cut(s) 1141, 1861, 2366, 3215
PstI CTGCAG 2 cut(s) 349, 1501
PstNI CAGNNNCTG 1 cut(s) 710
PsuI RGATCY 4 cut(s) 296, 2076, 2624, 2737
PvuII CAGCTG 2 cut(s) 877, 1502
RsaI GTAC 4 cut(s) 927, 1867, 2356, 2690
RsaNI GTAC 4 cut(s) 926, 1866, 2355, 2689
RseI CAYNNNNRTG 3 cut(s) 1266, 2571, 3161
SacII CCGCGG 2 cut(s) 570, 2744
SapI GCTCTTC 2 cut(s) 1751, 2958
SatI GCNGC 8 cut(s) 345, 919, 956, 1500, 1703, 2467, 2730, 3095
Sau96I GGNCC 4 cut(s) 1141, 1861, 2366, 3215
ScaI AGTACT 1 cut(s) 2690
SchI GAGTC 3 cut(s) 38, 266, 1322
ScrFI CCNGG 9 cut(s) 377, 687, 784, 1110, 1310, 1776, 2823, 3061, 3608
SduI GDGCHC 2 cut(s) 1000, 1975
SfcI CTRYAG 5 cut(s) 345, 507, 702, 1497, 2808
Sfr303I CCGCGG 2 cut(s) 570, 2744
SgrBI CCGCGG 2 cut(s) 570, 2744
SinI GGWCC 2 cut(s) 1861, 3215
SmiMI CAYNNNNRTG 3 cut(s) 1266, 2571, 3161
SmlI CTYRAG 4 cut(s) 739, 960, 1098, 3036
SmoI CTYRAG 4 cut(s) 739, 960, 1098, 3036
SnaBI TACGTA 1 cut(s) 2382
SphI GCATGC 1 cut(s) 2016
SseBI AGGCCT 1 cut(s) 1900
SspMI CTAG 8 cut(s) 71, 1559, 1890, 2009, 2471, 2480, 2699, 3264
StuI AGGCCT 1 cut(s) 1900
StyD4I CCNGG 9 cut(s) 375, 685, 782, 1108, 1308, 1774, 2821, 3059, 3606
StyI CCWWGG 2 cut(s) 1464, 1766
TaiI ACGT 5 cut(s) 253, 1205, 1871, 1996, 2384
TaqI TCGA 6 cut(s) 854, 1662, 1906, 1988, 2977, 3600
TaqII GACCGA 1 cut(s) 2214
TatI WGTACW 1 cut(s) 2688
TauI GCSGC 3 cut(s) 958, 2469, 3097
TfiI GAWTC 9 cut(s) 335, 425, 682, 1004, 1226, 1487, 2087, 2114, 2474
TseFI GTSAC 7 cut(s) 134, 706, 977, 1492, 2195, 2603, 2712
TseI GCWGC 5 cut(s) 344, 918, 1499, 1702, 2729
Tsp45I GTSAC 7 cut(s) 134, 706, 977, 1492, 2195, 2603, 2712
TspGWI ACGGA 4 cut(s) 1587, 2172, 2563, 3381
Van91I CCANNNNNTGG 2 cut(s) 899, 2537
VneI GTGCAC 2 cut(s) 996, 1971
VpaK11BI GGWCC 2 cut(s) 1861, 3215
VspI ATTAAT 1 cut(s) 2456
XapI RAATTY 9 cut(s) 850, 934, 1150, 2273, 2797, 2875, 2906, 3166, 3320
XbaI TCTAGA 1 cut(s) 2479
XceI RCATGY 3 cut(s) 56, 2016, 2837
XcmI CCANNNNNNNNNTGG 1 cut(s) 761
XmnI GAANNNNTTC 1 cut(s) 1202
XspI CTAG 8 cut(s) 71, 1559, 1890, 2009, 2471, 2480, 2699, 3264
ZrmI AGTACT 1 cut(s) 2690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.