Rh1BG284700

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0003478
Physical Location & Seq
Reverse (-)
42078690 .. 42085600
6911 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG284700.1

Sequence Viewer

Length: 3624 bp
ATGGACGAAAGCAAAGACAAGGGTGTTGCTGGTGAAATTGTAGCTGGGGCTGTTGGGAGTCAGGGTCAGGTTAGTGAGGAAGGTGGACCTTGTAATGGGGATGATCTAATGGTGGAGGTTTTCGGGTCTAATGTGTATGTGGGTGGAGTTTGTACTAGTGGGGGAGGGGATGAGGAGGATAAGGAGGTGGGTCGTGAGGAGTCGGTTGAGGTTGATATGAGTTCGGGAAGGAATGCGGGGTCTGTGGGTGGAGATGGTGGTGATGCGGGTTCGGGTGGGAAGGTAGAGTCGGTGGAAGAAAGAAGGGGAGTGGTTGCGGTGGAGGAGGTAGGGGTGGTGGTTAGGGAGGAGAGTTTGAAGGGAGCAGGGGCAGCTGCTGTTGCTGGTTCGGCTGGTGGAGAAACACAGGTAGTTGATGTTGAGAAAGCTGAAAAAGAAAGGAAACAAATTGGAGGACTTCTGGAGGAAGTTTTTGGGCGGGGGAATACGGATGGTGTGAAACAAGAAAAGAAGGTAGTTGATGGTGGGGCTGATTTGAAGGTACAGAAAGTTAGTGTTGCAGATGATGAAGTAAGGAATCCTGGAATTGACACTGTAGCTACAAGTTCTTCGGAAACAGTGGAGAATTCTTGTGTTCGGACGGAAAAAGCTCCAGTTGTCAGTGAAGAAGGTTCGAGTCCCAAACCCTTGAATGGACAAAGGCAAGTATCTGCTGGTGGTGGTGATGTTTCAGGCACGGACAAAGGAGATACTGTGACTTCCGAAGTTGCAGGTGTAGAGATTGGTAAAATTGATGAAAGTAATTCAGTAGGAAAACAGCAGGTTAAGATTGAGTCGCTAGGTGGAAACATCCATACTCACATCAATCTACCTGCACATACAGTTGCTTCGTCCCTGCCAGCTAAAGTAGTTCGTGTAGGTGAAATTGCTGTAACAGATGGAGAGGATCTCTTGAATTCTGAAAAAGATCATTTGAAATTAGAAGAGAGTGTAGAGAACATGTTTCATGATATTGCTCAGGTTGGATCAAATACTTCATCTCTGCCAGCCAAACTTCATCCTGGATGCATAGATCGAGAGGGTCTTTTGAATTTTGAAAGTCATCGGCACTTGAAACCCGAAGAGATTGTGGACAAGAACATGGTCCATGATATGGCCCAGGTTGAATTAAATGGAGAACAAGAAATGGAAGTGGACAACCAAGTTAATGATGCTGAACATGGTGAGTTAGTTTCAGGAAATGATCAGAATTCAAAAATTGAGAAATCATGCATGAACTCTGTTGGGAAGGATCAAAAGTTGAAGTTTGAAGAGAGTTTAAACAATAGTGTGACCACTGACCATGCTCAGTCCAAGACCAATGTTGTACATGAGGTTGAGGTAACTGAAGCTGATATGGATGGTTTATATGGAGAAAGCCTAGACATGGAAGTGGACAGAGATGCTAGTCATAGGGAACAACCAAAACCCAGTGAAGAGAAAATTGTTAATCAAGAAGCTATGCAGCCTGAAAGCTCTGAAATAGTGCCTCAGCTTAGATATGAGCTGCCACAAAAAAGCGAAGGTACATTTTCTGTCTCAGATTTAGTTTGGGGTAAAGTGAAGAGCCATCCATGGTGGCCTGGACAGATATTTGATTTTATGGCTGCATCTGAGAAGGCAATGAAACATCATAAGAAGGACTGTTTTTTGGTAGCATATTTTGGGGACCGAACATTTGCTTGGAATGAAGTATCTAGTTTAAAGCCTTTCCGATCTTATTTCTCCCAGGCAGAGAAGCAGTGCAATTCAGAAACATTCCACAAAGCTGTCAATTGTGTTCTGGAAGAAGTTTCAAGACGTGTAGAATTGGGTCTATCATGCTCATGCATACCAAAAGATGTTTATGAAAAGATCAGATTCCAGATTATTGAAAATGCTGGGATATGCCATGAATCTAGTAGAGTGGACGGAGTAGATGAATCTGCAAGTGCAAGTTCATTTGAATCTGATAAACTACTTAAATATGTAAAAGCTTTAGCACGGTTCCCATCTGGCGGCAGTGACAAATTAGAACTTGTCATTGCCAAGGCTCATTTGCTGGCATATTTTCGTTTAAAGGGTTATTGTAGTTTGCCTGAATTCCAGTTTTGTGGAGATTTGTTGGAGAGTAAAACAGATAATTCGCTCTCTGGGGACAAAACATGTCCGGGTGAAATTACCGAGCATGCGACTTATATTGGGAAGGATGATAAGAAGACTGGACCGGAGATTGAGGAACTCAAGTCTAGCACTTCTCATAAACGCAAACATAATCTAAGAGAAGGAGCTTATGCTAAAATGAAAGAAAGAAGCCTGTCTGAATTAATGGGTGGCGAAACAGGTTCTCCAGATGGTAATGATTGGTTGGATGCGAAGGCTTCGCCATCATCTGGCAAGAGACGGAAGGGTGCTGAGTATCAGGCTGCTGATTTAGCCACACAAGATGGGAGGAAAGCTGTTTCCCATTCAACAGTTTCAAACACAACACCTTCACCAAAACCATCGTTTAAGATAGGTGAATGTATCCAGAGAGCTGCAAGCCAACTATCAGGGTCTACTATAGTGAAGAGCAGCACTGACAGATCTGCTGTGCAGGGATCTGATGTTCCCTTCCAGAGTTCTGATGATGCTCTTAGAGGAGTGAATAATACAACAAAGTACTCATCTCTAGATGAATTGCTGTCACAACTTCGGTCAGCAGCAGAAGAACCTCTGAAGGAATACAATTCCTTGAGTACTATTGTCAATTTCTTCTCTGATTTTAGGAATTCTGTGGTTGTTGGCCAGAAATCTGGGGTGGTGCTTTCAGTTGTAGACAAAGTTGGTGGTAAAAAGAGGAAGTCAAATTCTGTGCATGGGTTACCTGAGACTTTTGAATTTGATGATATGAATGACACTTATTGGACAGACATGGTAATCCAAAATGGTGGAGAAGAGCAAGCATCACGGAAAAGAAAAACGAAGTATCAACCTGTCGTCCTTGGGCAACCAGAAAAGCCCCCTCAAGTGGGTCGCAGGCCATACACCAGGAAGAAGTTTTCTCAAGGGAGTCAAGATTTGCCTCCGGAGAAACCTGTTGGCTATGTGGATGAGAATGCACCAGCAGAACTTGTAATGAGCTTCTCTGAGGTGAGCTCTATTCCTTCAGAAACAAACCTGAATAAAATGTTCAAGCGCTTTGGACCTCTGAAGGAATATGAAACAGAAGTTGATAGGGAGAGCAGCCGTGCTAGAGTTGTTTTTAAGAGATGTTCTGATGCAGAAGTTGCTTGCAATAGTGCTGGGAAATTCAACATCTTTGGACAGATCACTGTAAATTACCAGCTCAACTATACTCCCTCTCAGCTCAACTACACGCCATCTATTACGTTCGGTGCATCTACCAGTGCTGCAGCCCAGGATCAGGAAGTGCAACTTGTTTTCCCCTCCCATGATCATGAGATGCATCTTGATCTCTCCACCCATGACCAGATGCAGCTTGATCTTTCCACCCATGACCAGATGCAGCTTGACCTTTCCACCCATGACCACATGCAACTTGATCTTTCAACCCATGACGAGATGCAACTAGACCTCTCAACATTTGAAGTTAATCTGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

1207

Amino Acids

131.29

Weight (kDa)

4.97

Isoelectric Point (pI)

40.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 528 - 610 4.3e-17 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 761
Acc36I ACCTGC 3 cut(s) 761, 811, 880
AccB7I CCANNNNNTGG 2 cut(s) 1153, 2411
AccI GTMKAC 2 cut(s) 2576, 2835
AccIII TCCGGA 1 cut(s) 3085
AciI CCGC 5 cut(s) 236, 266, 317, 478, 2037
AclWI GGATC 5 cut(s) 954, 1033, 1299, 2626, 3429
AcoI YGGCCR 1 cut(s) 2803
AcsI RAATTY 9 cut(s) 625, 955, 1090, 1249, 2120, 2788, 2866, 2897, 3308
AcuI CTGAAG 4 cut(s) 1407, 2756, 3150, 3230
AfaI GTAC 6 cut(s) 154, 543, 1368, 1567, 2681, 2758
AfeI AGCGCT 1 cut(s) 3197
AfiI CCNNNNNNNGG 9 cut(s) 95, 692, 1153, 1426, 2036, 2411, 3028, 3029, 3424
AflIII ACRYGT 3 cut(s) 999, 1840, 2183
AhlI ACTAGT 1 cut(s) 155
AjiI CACGTC 1 cut(s) 1841
AjnI CCWGG 7 cut(s) 580, 1060, 1158, 1621, 1767, 3047, 3417
AjuI GAANNNNNNNTTGG 2 cut(s) 1705, 1737
AloI GAACNNNNNNTCC 2 cut(s) 2610, 2642
Alw21I GWGCWC 1 cut(s) 3158
Alw26I GTCTC 3 cut(s) 1582, 2413, 2882
AlwI GGATC 5 cut(s) 954, 1033, 1299, 2626, 3429
AlwNI CAGNNNCTG 1 cut(s) 377
Aor13HI TCCGGA 1 cut(s) 3085
Aor51HI AGCGCT 1 cut(s) 3197
AoxI GGCC 4 cut(s) 1155, 1619, 2803, 3038
ApoI RAATTY 9 cut(s) 625, 955, 1090, 1249, 2120, 2788, 2866, 2897, 3308
ArsI GACNNNNNNTTYG 2 cut(s) 1700, 1732
AseI ATTAAT 1 cut(s) 2345
AspLEI GCGC 1 cut(s) 3198
AspS9I GGNCC 6 cut(s) 86, 1144, 1156, 1708, 2243, 3203
AsuC2I CCSGG 1 cut(s) 2190
AsuHPI GGTGA 9 cut(s) 44, 272, 734, 932, 1235, 2204, 2505, 2549, 3163
AvaII GGWCC 5 cut(s) 86, 1144, 1708, 2243, 3203
BalI TGGCCA 1 cut(s) 2805
BanII GRGCYC 1 cut(s) 3158
BbsI GAAGAC 1 cut(s) 2243
Bbv12I GWGCWC 1 cut(s) 3158
BbvCI CCTCAGC 1 cut(s) 1530
BceAI ACGGC 1 cut(s) 3231
BciT130I CCWGG 7 cut(s) 582, 1062, 1160, 1623, 1769, 3049, 3419
BciVI GTATCC 1 cut(s) 2555
BclI TGATCA 2 cut(s) 1243, 3454
BcnI CCSGG 1 cut(s) 2190
BcoDI GTCTC 3 cut(s) 1582, 2413, 2882
BcuI ACTAGT 1 cut(s) 155
BfmI CTRYAG 3 cut(s) 594, 2580, 3411
BfoI RGCGCY 1 cut(s) 3199
BfuAI ACCTGC 3 cut(s) 761, 811, 880
BfuI GTATCC 1 cut(s) 2555
BglII AGATCT 1 cut(s) 2603
BmcAI AGTACT 2 cut(s) 2681, 2758
Bme1390I CCNGG 8 cut(s) 582, 1062, 1160, 1623, 1769, 2190, 3049, 3419
Bme18I GGWCC 5 cut(s) 86, 1144, 1708, 2243, 3203
BmgBI CACGTC 1 cut(s) 1841
BmgT120I GGNCC 6 cut(s) 86, 1144, 1156, 1708, 2243, 3203
BmiI GGNNCC 2 cut(s) 1709, 2027
BmrFI CCNGG 8 cut(s) 582, 1062, 1160, 1623, 1769, 2190, 3049, 3419
BmrI ACTGGG 1 cut(s) 1464
BmuI ACTGGG 1 cut(s) 1464
BpiI GAAGAC 1 cut(s) 2243
BplI GAGNNNNNCTC 4 cut(s) 933, 965, 3140, 3172
BpmI CTGGAG 3 cut(s) 482, 636, 2352
Bpu10I CCTNAGC 2 cut(s) 1017, 1530
BpuEI CTTGAG 4 cut(s) 2246, 2773, 3009, 3048
BpuMI CCSGG 1 cut(s) 2190
BsaJI CCNNGG 6 cut(s) 1158, 1613, 1767, 2067, 3001, 3417
BsaWI WCCGGW 2 cut(s) 2245, 3085
BsaXI ACNNNNNCTCC 6 cut(s) 49, 79, 738, 768, 2350, 2380
Bsc4I CCNNNNNNNGG 9 cut(s) 95, 692, 1153, 1426, 2036, 2411, 3028, 3029, 3424
Bse1I ACTGG 5 cut(s) 653, 1470, 2125, 2245, 3405
Bse3DI GCAATG 2 cut(s) 1668, 2061
BseAI TCCGGA 1 cut(s) 3085
BseBI CCWGG 7 cut(s) 582, 1062, 1160, 1623, 1769, 3049, 3419
BseDI CCNNGG 6 cut(s) 1158, 1613, 1767, 2067, 3001, 3417
BseLI CCNNNNNNNGG 9 cut(s) 95, 692, 1153, 1426, 2036, 2411, 3028, 3029, 3424
BseMI GCAATG 2 cut(s) 1668, 2061
BseMII CTCAG 9 cut(s) 1031, 1361, 1544, 1593, 1644, 2424, 2877, 3138, 3377
BseNI ACTGG 5 cut(s) 653, 1470, 2125, 2245, 3405
BseRI GAGGAG 5 cut(s) 188, 212, 338, 362, 2673
BseYI CCCAGC 3 cut(s) 44, 1919, 3302
BsgI GTGCAG 2 cut(s) 858, 2633
BshFI GGCC 4 cut(s) 1157, 1621, 2805, 3040
BsiHKAI GWGCWC 1 cut(s) 3158
BsiSI CCGG 3 cut(s) 2189, 2246, 3086
BslFI GGGAC 4 cut(s) 663, 877, 1721, 2189
BslI CCNNNNNNNGG 9 cut(s) 95, 692, 1153, 1426, 2036, 2411, 3028, 3029, 3424
BsmAI GTCTC 3 cut(s) 1582, 2413, 2882
BsmBI CGTCTC 1 cut(s) 2413
BsmFI GGGAC 4 cut(s) 663, 877, 1721, 2189
BsmI GAATGC 2 cut(s) 238, 3121
BsnI GGCC 4 cut(s) 1157, 1621, 2805, 3040
Bsp1286I GDGCHC 1 cut(s) 3158
Bsp13I TCCGGA 1 cut(s) 3085
Bsp1407I TGTACA 1 cut(s) 1366
Bsp19I CCATGG 1 cut(s) 1613
BspACI CCGC 5 cut(s) 236, 266, 317, 478, 2037
BspANI GGCC 4 cut(s) 1157, 1621, 2805, 3040
BspCNI CTCAG 9 cut(s) 1030, 1360, 1543, 1592, 1645, 2425, 2878, 3139, 3376
BspEI TCCGGA 1 cut(s) 3085
BspHI TCATGA 2 cut(s) 1006, 3457
BspLI GGNNCC 2 cut(s) 1709, 2027
BspMAI CTGCAG 1 cut(s) 3415
BspMI ACCTGC 3 cut(s) 761, 811, 880
BspPI GGATC 5 cut(s) 954, 1033, 1299, 2626, 3429
BspQI GCTCTTC 3 cut(s) 1598, 2582, 2949
BsrDI GCAATG 2 cut(s) 1668, 2061
BsrGI TGTACA 1 cut(s) 1366
BsrI ACTGG 5 cut(s) 653, 1470, 2125, 2245, 3405
BssECI CCNNGG 6 cut(s) 1158, 1613, 1767, 2067, 3001, 3417
BssT1I CCWWGG 3 cut(s) 1613, 2067, 3001
Bst2UI CCWGG 7 cut(s) 582, 1062, 1160, 1623, 1769, 3049, 3419
Bst4CI ACNGT 8 cut(s) 595, 619, 754, 883, 1685, 2025, 2494, 3334
Bst6I CTCTTC 7 cut(s) 978, 1116, 1305, 1470, 1598, 2582, 2949
BstAPI GCANNNNNTGC 1 cut(s) 3287
BstAUI TGTACA 1 cut(s) 1366
BstC8I GCNNGC 8 cut(s) 900, 1047, 2082, 2208, 2560, 2961, 3038, 3292
BstDSI CCRYGG 1 cut(s) 1613
BstEII GGTNACC 1 cut(s) 2880
BstH2I RGCGCY 1 cut(s) 3199
BstHHI GCGC 1 cut(s) 3198
BstMAI GTCTC 3 cut(s) 1582, 2413, 2882
BstMWI GCNNNNNNNGC 5 cut(s) 371, 380, 389, 2453, 3287
BstNI CCWGG 7 cut(s) 582, 1062, 1160, 1623, 1769, 3049, 3419
BstNSI RCATGY 4 cut(s) 1003, 2187, 2210, 3556
BstPI GGTNACC 1 cut(s) 2880
BstSCI CCNGG 8 cut(s) 580, 1060, 1158, 1621, 1767, 2188, 3047, 3417
BstSFI CTRYAG 3 cut(s) 594, 2580, 3411
BstV2I GAAGAC 1 cut(s) 2243
BstX2I RGATCY 3 cut(s) 946, 2603, 2618
BstXI CCANNNNNNTGG 3 cut(s) 2132, 2813, 2948
BstYI RGATCY 3 cut(s) 946, 2603, 2618
BsuI GTATCC 1 cut(s) 2555
BsuRI GGCC 4 cut(s) 1157, 1621, 2805, 3040
BtgI CCRYGG 1 cut(s) 1613
BtrI CACGTC 1 cut(s) 1841
BtsI GCAGTG 2 cut(s) 1787, 2047
BveI ACCTGC 3 cut(s) 761, 811, 880
Cac8I GCNNGC 8 cut(s) 900, 1047, 2082, 2208, 2560, 2961, 3038, 3292
CaiI CAGNNNCTG 1 cut(s) 377
CciI TCATGA 2 cut(s) 1006, 3457
CfoI GCGC 1 cut(s) 3198
Cfr13I GGNCC 6 cut(s) 86, 1144, 1156, 1708, 2243, 3203
Csp6I GTAC 6 cut(s) 153, 542, 1367, 1566, 2680, 2757
CspCI CAANNNNNGTGG 2 cut(s) 2827, 2862
CviQI GTAC 6 cut(s) 153, 542, 1367, 1566, 2680, 2757
DraI TTTAAA 3 cut(s) 1320, 1743, 2097
EaeI YGGCCR 1 cut(s) 2803
Eam1104I CTCTTC 7 cut(s) 978, 1116, 1305, 1470, 1598, 2582, 2949
EarI CTCTTC 7 cut(s) 978, 1116, 1305, 1470, 1598, 2582, 2949
Ecl136II GAGCTC 1 cut(s) 3156
Eco130I CCWWGG 3 cut(s) 1613, 2067, 3001
Eco24I GRGCYC 1 cut(s) 3158
Eco47I GGWCC 5 cut(s) 86, 1144, 1708, 2243, 3203
Eco47III AGCGCT 1 cut(s) 3197
Eco53kI GAGCTC 1 cut(s) 3156
Eco57I CTGAAG 4 cut(s) 1407, 2756, 3150, 3230
Eco91I GGTNACC 1 cut(s) 2880
EcoICRI GAGCTC 1 cut(s) 3156
EcoO65I GGTNACC 1 cut(s) 2880
EcoRI GAATTC 5 cut(s) 625, 955, 1249, 2120, 2788
EcoRII CCWGG 7 cut(s) 580, 1060, 1158, 1621, 1767, 3047, 3417
EcoT14I CCWWGG 3 cut(s) 1613, 2067, 3001
EcoT22I ATGCAT 4 cut(s) 1070, 1274, 1871, 3468
EcoT38I GRGCYC 1 cut(s) 3158
ErhI CCWWGG 3 cut(s) 1613, 2067, 3001
Esp3I CGTCTC 1 cut(s) 2413
FalI AAGNNNNNCTT 2 cut(s) 3420, 3452
FaqI GGGAC 4 cut(s) 663, 877, 1721, 2189
FauI CCCGC 3 cut(s) 229, 259, 471
FbaI TGATCA 2 cut(s) 1243, 3454
FblI GTMKAC 2 cut(s) 2576, 2835
FriOI GRGCYC 1 cut(s) 3158
GlaI GCGC 1 cut(s) 3197
GsaI CCCAGC 3 cut(s) 48, 1923, 3306
GsuI CTGGAG 3 cut(s) 482, 636, 2352
HaeII RGCGCY 1 cut(s) 3199
HaeIII GGCC 4 cut(s) 1157, 1621, 2805, 3040
HapII CCGG 3 cut(s) 2189, 2246, 3086
HhaI GCGC 1 cut(s) 3198
Hin6I GCGC 1 cut(s) 3196
HinP1I GCGC 1 cut(s) 3196
HindIII AAGCTT 1 cut(s) 2013
HpaII CCGG 3 cut(s) 2189, 2246, 3086
HphI GGTGA 9 cut(s) 44, 272, 734, 932, 1235, 2204, 2505, 2549, 3163
Hpy166II GTNNAC 7 cut(s) 86, 1132, 1195, 1435, 1948, 2577, 2836
Hpy8I GTNNAC 7 cut(s) 86, 1132, 1195, 1435, 1948, 2577, 2836
HpyCH4III ACNGT 8 cut(s) 595, 619, 754, 883, 1685, 2025, 2494, 3334
HpyCH4IV ACGT 2 cut(s) 1840, 3389
HpyF10VI GCNNNNNNNGC 5 cut(s) 371, 380, 389, 2453, 3287
HpySE526I ACGT 2 cut(s) 1840, 3389
HspAI GCGC 1 cut(s) 3196
Kpn2I TCCGGA 1 cut(s) 3085
Ksp22I TGATCA 2 cut(s) 1243, 3454
LguI GCTCTTC 3 cut(s) 1598, 2582, 2949
LmnI GCTCC 3 cut(s) 362, 655, 2306
MaeII ACGT 2 cut(s) 1840, 3389
MaeIII GTNAC 7 cut(s) 754, 931, 1330, 1381, 2042, 2703, 2880
MfeI CAATTG 1 cut(s) 1813
MflI RGATCY 3 cut(s) 946, 2603, 2618
MhlI GDGCHC 1 cut(s) 3158
MlsI TGGCCA 1 cut(s) 2805
MluNI TGGCCA 1 cut(s) 2805
MlyI GAGTC 6 cut(s) 67, 209, 296, 685, 842, 3079
MmeI TCCRAC 3 cut(s) 1003, 2124, 2367
Mox20I TGGCCA 1 cut(s) 2805
Mph1103I ATGCAT 4 cut(s) 1070, 1274, 1871, 3468
MroI TCCGGA 1 cut(s) 3085
MscI TGGCCA 1 cut(s) 2805
MslI CAYNNNNRTG 3 cut(s) 1430, 1864, 3456
Msp20I TGGCCA 1 cut(s) 2805
MspA1I CMGCKG 1 cut(s) 374
MspI CCGG 3 cut(s) 2189, 2246, 3086
MspR9I CCNGG 8 cut(s) 582, 1062, 1160, 1623, 1769, 2190, 3049, 3419
MssI GTTTAAAC 1 cut(s) 1320
MunI CAATTG 1 cut(s) 1813
Mva1269I GAATGC 2 cut(s) 238, 3121
MvaI CCWGG 7 cut(s) 582, 1062, 1160, 1623, 1769, 3049, 3419
MwoI GCNNNNNNNGC 5 cut(s) 371, 380, 389, 2453, 3287
NciI CCSGG 1 cut(s) 2190
NcoI CCATGG 1 cut(s) 1613
NlaIV GGNNCC 2 cut(s) 1709, 2027
NmuCI GTSAC 4 cut(s) 754, 1330, 2042, 2703
NsiI ATGCAT 4 cut(s) 1070, 1274, 1871, 3468
NspI RCATGY 4 cut(s) 1003, 2187, 2210, 3556
PaeI GCATGC 1 cut(s) 2210
PagI TCATGA 2 cut(s) 1006, 3457
PaqCI CACCTGC 1 cut(s) 761
PciI ACATGT 2 cut(s) 999, 2183
PciSI GCTCTTC 3 cut(s) 1598, 2582, 2949
PctI GAATGC 2 cut(s) 238, 3121
PfeI GAWTC 5 cut(s) 577, 1899, 1934, 1961, 1985
PflMI CCANNNNNTGG 2 cut(s) 1153, 2411
PfoI TCCNGGA 2 cut(s) 580, 1060
PleI GAGTC 6 cut(s) 66, 208, 295, 684, 841, 3078
PmeI GTTTAAAC 1 cut(s) 1320
PpsI GAGTC 6 cut(s) 66, 208, 295, 684, 841, 3078
PscI ACATGT 2 cut(s) 999, 2183
PshBI ATTAAT 1 cut(s) 2345
Psp124BI GAGCTC 1 cut(s) 3158
Psp6I CCWGG 7 cut(s) 580, 1060, 1158, 1621, 1767, 3047, 3417
PspEI GGTNACC 1 cut(s) 2880
PspFI CCCAGC 3 cut(s) 44, 1919, 3302
PspGI CCWGG 7 cut(s) 580, 1060, 1158, 1621, 1767, 3047, 3417
PspN4I GGNNCC 2 cut(s) 1709, 2027
PspPI GGNCC 6 cut(s) 86, 1144, 1156, 1708, 2243, 3203
PstI CTGCAG 1 cut(s) 3415
PstNI CAGNNNCTG 1 cut(s) 377
PsuI RGATCY 3 cut(s) 946, 2603, 2618
PvuII CAGCTG 1 cut(s) 374
RsaI GTAC 6 cut(s) 154, 543, 1368, 1567, 2681, 2758
RsaNI GTAC 6 cut(s) 153, 542, 1367, 1566, 2680, 2757
RseI CAYNNNNRTG 3 cut(s) 1430, 1864, 3456
SacI GAGCTC 1 cut(s) 3158
SapI GCTCTTC 3 cut(s) 1598, 2582, 2949
Sau96I GGNCC 6 cut(s) 86, 1144, 1156, 1708, 2243, 3203
ScaI AGTACT 2 cut(s) 2681, 2758
SchI GAGTC 6 cut(s) 67, 209, 296, 685, 842, 3079
ScrFI CCNGG 8 cut(s) 582, 1062, 1160, 1623, 1769, 2190, 3049, 3419
SduI GDGCHC 1 cut(s) 3158
SfcI CTRYAG 3 cut(s) 594, 2580, 3411
SinI GGWCC 5 cut(s) 86, 1144, 1708, 2243, 3203
SmiMI CAYNNNNRTG 3 cut(s) 1430, 1864, 3456
SmlI CTYRAG 4 cut(s) 2261, 2752, 3024, 3063
SmoI CTYRAG 4 cut(s) 2261, 2752, 3024, 3063
SpeI ACTAGT 1 cut(s) 155
SphI GCATGC 1 cut(s) 2210
SsiI CCGC 5 cut(s) 236, 266, 317, 478, 2037
SstI GAGCTC 1 cut(s) 3158
StyD4I CCNGG 8 cut(s) 580, 1060, 1158, 1621, 1767, 2188, 3047, 3417
StyI CCWWGG 3 cut(s) 1613, 2067, 3001
TaaI ACNGT 8 cut(s) 595, 619, 754, 883, 1685, 2025, 2494, 3334
TaiI ACGT 2 cut(s) 1843, 3392
TaqI TCGA 2 cut(s) 674, 1075
TaqII GACCGA 2 cut(s) 1725, 2703
TatI WGTACW 4 cut(s) 152, 1366, 2679, 2756
TauI GCSGC 1 cut(s) 2040
TfiI GAWTC 5 cut(s) 577, 1899, 1934, 1961, 1985
TseFI GTSAC 4 cut(s) 754, 1330, 2042, 2703
Tsp45I GTSAC 4 cut(s) 754, 1330, 2042, 2703
TspGWI ACGGA 6 cut(s) 503, 656, 752, 1965, 2437, 2983
Van91I CCANNNNNTGG 2 cut(s) 1153, 2411
VpaK11BI GGWCC 5 cut(s) 86, 1144, 1708, 2243, 3203
VspI ATTAAT 1 cut(s) 2345
XapI RAATTY 9 cut(s) 625, 955, 1090, 1249, 2120, 2788, 2866, 2897, 3308
XbaI TCTAGA 1 cut(s) 2689
XceI RCATGY 4 cut(s) 1003, 2187, 2210, 3556
XmiI GTMKAC 2 cut(s) 2576, 2835
ZrmI AGTACT 2 cut(s) 2681, 2758
Zsp2I ATGCAT 4 cut(s) 1070, 1274, 1871, 3468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.