RLG00000027509

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0003478
Physical Location & Seq
Forward (+)
12303047 .. 12307258
4212 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027509

Sequence Viewer

Length: 3669 bp
ATGGACGAAAGCAAAGACAAGGGTGTGGCTGGTGAAATTGTAGCTCGGGCTGTTGGGAGTCAGGGTCAGGTTAGTGAGGAAGGTGCGCCTTGTAATGGGGATGATCTAATGGTGGAGGTTTTCGGGTCTAATGTGTATGTGGGTGGAGTTTGTACTAGTGGGAGAGGGGATGAGGAGGATAAGGAGGTGGGTCGTGAGGAGTCGGTTGAGGTTGATATGAGTTCAGGAAGGAATGCGGGGTCTGTGGGTGGAGATGGTGGTGAAGCAGGTTCGGGTGGGAAGGTAGAGTCGGTGGAAGAAAGAAGGGGAGTGGTTGCGGTGGAGGAGGTTGGGGTGGTGGTTAGGGAGGAGAGTTTGAAGGGAGCAGGGGCAGCTGTTGTTGCTGGTTCGGCTGGTGGAGAAACACAGGTAGTTGATGTTGAGAAAGCTGAAAAAGAAAGGAAACAAATTGGATTACGGAGGGAAGTTTTTGGGCGTGGGATTACGGATGGTGTGAAACAAGAAAAGAAGGTAGTTGATGGTGGGGCTGATTTGAAGGTACAGAAAGTTGGTGTTGCAGATAATGAAGTAAGGAATCCTGGAATTGACACTGTAGCTGCAAGTTCTTCGGAAACAGTGGAGAATTCTTATGTTTGGACCGGAGTTGTGGAAAAAGCTCCAGTTGTCAGTGAAGAAGGTTTGAGTCCCAAACCCTTGAATGGACAAAGGCAAGTTTCTGCTGGTGGCGGTGATGTTTCATGCACGGACAAAGGAGATACTGTGACTTCCAAAGTTGCAGGTGTACAGATTGGTAAAATTGATGAAAGTAATTCAGTAGGAAAACAGCAGGTTAAGATTGAGTCGCTAGGTGGAAACATCCATACTCACATCAATCTACCTGCACATACAGTTGCTTCGTCCCTGCCAGCTAAAGTAGTTCCTGTAGGTGAAATTGCTGTAACAGATGGAGAGGATCTCTTGAATTCTGAAAAAGATCATTTGAAATTAGAAGAGAGTGTAGAGAACATGTTTCATGATATTGCTCAGGTTGGATCAAATACTTCATCTCTGCCAGCAAAACTTCATCCTGGATGCATAGATCGAGAGGGTCTTTTGAATTTTGAAAGTCATCAGCACTTGAAACCCGAAGAGATTGTAGACAAGAACATGGTCCATGATATGGCCCTGGTTGAATTAAATGGAGGACAAGAAATGGAAGTGGACAACCAAGTTAACGATGCTGAACATGGTGAGTTAGTTTCAGGAATTGATCAGAACTCAAAAATTGAGAAATCATGCGTGAACTCTGTTGGGAAGGATCAAAAGTTGAAGTTTGAAGAGAGTTTAAACAATAGTGTGACCACTGACCTTGCTCAGTCCAAGACCAATGTTGTACATGAGGTTGAGGTAACTGAAGCTGATATGGATGGTTTATATGGAGAAAGCCTAGACATGGAAGTGGACAGAGATGCTAGTCATAGGGAACAACCAAAACCCAGTGAAGAGAAAATTGTTATTCAAGAAGCTATGCAGCCTGAAGGCTCTGAAATAGTGCCTCAGCTTAGATATGAGCTCCCACAAAAAATTGAAGGTACTTTTTCTGTCTCAGATCTAGTTTGGGGTAAAGTGAAGAGCCATCCATGGTGGCCTGGACAGATATTTGATTTTATGGCTGCATCTGAGAAGGCAATGAAACATCATAAGAAGGACTGTTTTTTGGTAGCATATTTTGGGGACCGAACATTTGCTTGGAATGAAGTATCTAGTTTAAAGCCTTTCCGGTCTTATTTCTCCCAGGCAGAGAAGCAGTGCAATTCAGAAACATTCCACAAAGCTGTCAATTGTGTTCTGGAAGAAGTTTCAAGACGTGTAGAATTGGGTCTATCATGCTCATGCATACCAAAAGATGTTTATGAAAAGATCAGATTCCAGATTATTGAAAATGCTGGGATATGCCATGAATCGAGTAGAGTGGATGGAGTAGATGAATCTGCAAGTGCAAGTTCATTTGAATCTGATAAACTACTTAAATATGTAAAAGCTTTAGCACGGTTCCCATCTGGCGGCAGTGACAAATTAGAACTTGTCATTGCCAAGGCTCATTTGCTGGCATTTTTTCGTTTAAAGGGTTATTGTAGTTTGCCTGAATTCCAGTTTTGTGGAGATTTGTTGGAGAGTAAAACAGATAATTCGCTCTCTGGGGACAAAACATGTCCAGGTGAAATTACTGAGCATGCGACTTATATTGGGAAGGATGATAAGAAGACTGGACCGGAGATTGAGGAACTCAAGTCTAGCACTTCTCATAAACGCAAACATAATCTAAGAGAAGGAGTGTATGCTAAAATGAAAGAAAGAAGCCTGTCAGAATTAATGGGTGGCGAAACAGGTTCCCCAGATGGTAATGATTGGTTGGATGTGAAGGCTTTGCCATCATCTGGCAAGAGACGGAAGGGTGCTGAGTATCAGGCTGCTGATTTAGCCACACAAGATGGGAGGAAAGCTGTTTCTCATTCAACAGTTTCAAATACAACTCCTTCACCAAAACCATCGTTTAAGATTGGTGAATGTATCCAGAGAGCTGCAAGCCAACTATCAGGGTCTACTATAGTGAAGAGCAGCACTGACAGACCTGCTGTGCAGGGATCTGATGTTCCCTTCCAGAGTTCCGATGATACTCTTAGAGGAGTGAATAATACAACAAAGTACTCATCTCTAGATGAATTGCTGTCACAACTTCGGTCAGCAGCAGAAGAACCTCTGAAGGAATACAATTCCTTGAGTACTATTGTCAATTTCTTCTCTGATTTTAGGAATTCTGTGGTTGTTGGCCAGAAATCTGGGGTGGTGCTTTCAGTTGTAGACAAAGTTGGTGGTAAAAAGAGGAAGTCAAATTCTGTGCATGGGTTACCTGAGACTTTTGAATTTGATGATATGAATGACACTTATTGGACAGACATGGTAATCCAAAATGGTGGAGAAGAGCAAGCATCACGGAAAAGAAAAACGAAGTATCAACCTGTCGTCCTTGGGCAACCAGAAAAGCCCCCTCAAGTGGGTCGCAGGCCATACACCAGGAAGAAGTTTTCTCAAGGGAGTCAAGATTTGCCTCTGGAGAAACCTGTTGGCTATGTGGATGAGAATGCACCAGCAGAACTTGTAATGAGCTTCTCTGAGGTGAGCTCTATTCCTTCAGAAACAAACCTGAATAAAATGTTCAAGCGCTTTGGACCTCTGAAGGAATATGAAACAGAAGTTGATAGGGAGAGCAGCCGTGCTAGAGTTGTTTTTAAGCGATGTTCTGATGCAGAAGTTGCTTGCAATAGTGCTGGGAAATTCAACATCTTTGGACAGATTACTGTAAATTACCAGCTCAACTATACTCCTTCTCAGCTCAACTACACGCCATCTATTACGTTCGGTGCATCTACCAGTGCTGCAGCCCAGGATCAGGAAGTGCAACTTGTTTTCCCCTCCCATGATCATGAGATGCATCTTGATCTCTCCACCCATGACCAGATGCAGCTTGATCTTTCCACCCATGACCAGATGCAGCTTGACCTTTCCACCCATGACCACATGCAGCTTGATCTTTCCACCCATGACGAGATGCAACTAGACCTCTCAACATTTGAAGTTAATCTGTTAGGAGAGCGGATGCTGAAGAAAAGACAAATACAGGGCTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

1223

Amino Acids

133.43

Weight (kDa)

5.1

Isoelectric Point (pI)

38.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 530 - 612 4.3e-17 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 767
Acc36I ACCTGC 5 cut(s) 257, 767, 817, 886, 2620
AccB7I CCANNNNNTGG 2 cut(s) 1159, 2417
AccBSI CCGCTC 1 cut(s) 3634
AccI GTMKAC 3 cut(s) 1137, 2582, 2841
AciI CCGC 5 cut(s) 236, 317, 726, 2043, 3634
AclWI GGATC 5 cut(s) 960, 1039, 1305, 2632, 3435
AcoI YGGCCR 1 cut(s) 2809
AcsI RAATTY 8 cut(s) 622, 961, 1096, 2126, 2794, 2872, 2903, 3314
AcuI CTGAAG 6 cut(s) 1413, 1536, 2762, 3156, 3236, 3662
AfaI GTAC 7 cut(s) 154, 540, 783, 1374, 1573, 2687, 2764
AfeI AGCGCT 1 cut(s) 3203
AfiI CCNNNNNNNGG 9 cut(s) 95, 698, 1159, 1432, 2042, 2417, 3034, 3035, 3430
AflIII ACRYGT 3 cut(s) 1005, 1846, 2189
AhlI ACTAGT 1 cut(s) 155
AjiI CACGTC 1 cut(s) 1847
AjnI CCWGG 8 cut(s) 577, 1066, 1164, 1627, 1773, 2194, 3053, 3423
AjuI GAANNNNNNNTTGG 2 cut(s) 1711, 1743
AloI GAACNNNNNNTCC 2 cut(s) 2616, 2648
Alw21I GWGCWC 2 cut(s) 1554, 3164
Alw26I GTCTC 3 cut(s) 1588, 2419, 2888
AlwI GGATC 5 cut(s) 960, 1039, 1305, 2632, 3435
Ama87I CYCGRG 1 cut(s) 45
Aor51HI AGCGCT 1 cut(s) 3203
AoxI GGCC 4 cut(s) 1161, 1625, 2809, 3044
ApoI RAATTY 8 cut(s) 622, 961, 1096, 2126, 2794, 2872, 2903, 3314
ArsI GACNNNNNNTTYG 2 cut(s) 1706, 1738
AseI ATTAAT 1 cut(s) 2351
AspLEI GCGC 2 cut(s) 88, 3204
AspS9I GGNCC 6 cut(s) 636, 1150, 1162, 1714, 2249, 3209
AsuHPI GGTGA 9 cut(s) 44, 272, 740, 938, 1241, 2210, 2511, 2555, 3169
AvaI CYCGRG 1 cut(s) 45
AvaII GGWCC 5 cut(s) 636, 1150, 1714, 2249, 3209
BalI TGGCCA 1 cut(s) 2811
BanII GRGCYC 2 cut(s) 1554, 3164
BbsI GAAGAC 1 cut(s) 2249
Bbv12I GWGCWC 2 cut(s) 1554, 3164
BbvCI CCTCAGC 1 cut(s) 1536
BceAI ACGGC 1 cut(s) 3237
BcgI CGANNNNNNTGC 2 cut(s) 588, 622
BciT130I CCWGG 8 cut(s) 579, 1068, 1166, 1629, 1775, 2196, 3055, 3425
BciVI GTATCC 1 cut(s) 2561
BclI TGATCA 2 cut(s) 1249, 3460
BcoDI GTCTC 3 cut(s) 1588, 2419, 2888
BcuI ACTAGT 1 cut(s) 155
BfmI CTRYAG 4 cut(s) 591, 921, 2586, 3417
BfoI RGCGCY 1 cut(s) 3205
BfuAI ACCTGC 5 cut(s) 257, 767, 817, 886, 2620
BfuI GTATCC 1 cut(s) 2561
BglII AGATCT 1 cut(s) 1588
BmcAI AGTACT 2 cut(s) 2687, 2764
Bme1390I CCNGG 8 cut(s) 579, 1068, 1166, 1629, 1775, 2196, 3055, 3425
Bme18I GGWCC 5 cut(s) 636, 1150, 1714, 2249, 3209
BmeT110I CYCGRG 1 cut(s) 45
BmgBI CACGTC 1 cut(s) 1847
BmgT120I GGNCC 6 cut(s) 636, 1150, 1162, 1714, 2249, 3209
BmiI GGNNCC 3 cut(s) 1715, 2033, 2371
BmrFI CCNGG 8 cut(s) 579, 1068, 1166, 1629, 1775, 2196, 3055, 3425
BmrI ACTGGG 1 cut(s) 1470
BmuI ACTGGG 1 cut(s) 1470
BpiI GAAGAC 1 cut(s) 2249
BplI GAGNNNNNCTC 4 cut(s) 939, 971, 3146, 3178
BpmI CTGGAG 2 cut(s) 642, 3113
Bpu10I CCTNAGC 2 cut(s) 1023, 1536
BpuEI CTTGAG 4 cut(s) 2252, 2779, 3015, 3054
BsaJI CCNNGG 6 cut(s) 1164, 1619, 1773, 2073, 3007, 3423
BsaWI WCCGGW 3 cut(s) 638, 1758, 2251
BsaXI ACNNNNNCTCC 4 cut(s) 49, 79, 744, 774
Bsc4I CCNNNNNNNGG 9 cut(s) 95, 698, 1159, 1432, 2042, 2417, 3034, 3035, 3430
Bse1I ACTGG 5 cut(s) 659, 1476, 2131, 2251, 3411
Bse3DI GCAATG 2 cut(s) 1674, 2067
BseBI CCWGG 8 cut(s) 579, 1068, 1166, 1629, 1775, 2196, 3055, 3425
BseDI CCNNGG 6 cut(s) 1164, 1619, 1773, 2073, 3007, 3423
BseLI CCNNNNNNNGG 9 cut(s) 95, 698, 1159, 1432, 2042, 2417, 3034, 3035, 3430
BseMI GCAATG 2 cut(s) 1674, 2067
BseNI ACTGG 5 cut(s) 659, 1476, 2131, 2251, 3411
BseRI GAGGAG 5 cut(s) 188, 212, 338, 362, 2679
BseYI CCCAGC 2 cut(s) 1925, 3308
BsgI GTGCAG 2 cut(s) 864, 2639
BshFI GGCC 4 cut(s) 1163, 1627, 2811, 3046
BsiHKAI GWGCWC 2 cut(s) 1554, 3164
BsiHKCI CYCGRG 1 cut(s) 45
BsiSI CCGG 3 cut(s) 639, 1759, 2252
BslFI GGGAC 4 cut(s) 669, 883, 1727, 2195
BslI CCNNNNNNNGG 9 cut(s) 95, 698, 1159, 1432, 2042, 2417, 3034, 3035, 3430
BsmAI GTCTC 3 cut(s) 1588, 2419, 2888
BsmBI CGTCTC 1 cut(s) 2419
BsmFI GGGAC 4 cut(s) 669, 883, 1727, 2195
BsmI GAATGC 2 cut(s) 238, 3127
BsnI GGCC 4 cut(s) 1163, 1627, 2811, 3046
BsoBI CYCGRG 1 cut(s) 45
Bsp1286I GDGCHC 2 cut(s) 1554, 3164
Bsp1407I TGTACA 2 cut(s) 781, 1372
Bsp19I CCATGG 1 cut(s) 1619
BspACI CCGC 5 cut(s) 236, 317, 726, 2043, 3634
BspANI GGCC 4 cut(s) 1163, 1627, 2811, 3046
BspHI TCATGA 2 cut(s) 1012, 3463
BspLI GGNNCC 3 cut(s) 1715, 2033, 2371
BspMAI CTGCAG 1 cut(s) 3421
BspMI ACCTGC 5 cut(s) 257, 767, 817, 886, 2620
BspPI GGATC 5 cut(s) 960, 1039, 1305, 2632, 3435
BspQI GCTCTTC 3 cut(s) 1604, 2588, 2955
BsrBI CCGCTC 1 cut(s) 3634
BsrDI GCAATG 2 cut(s) 1674, 2067
BsrGI TGTACA 2 cut(s) 781, 1372
BsrI ACTGG 5 cut(s) 659, 1476, 2131, 2251, 3411
BssECI CCNNGG 6 cut(s) 1164, 1619, 1773, 2073, 3007, 3423
BssT1I CCWWGG 3 cut(s) 1619, 2073, 3007
Bst2UI CCWGG 8 cut(s) 579, 1068, 1166, 1629, 1775, 2196, 3055, 3425
Bst4CI ACNGT 8 cut(s) 592, 616, 760, 889, 1691, 2031, 2500, 3340
Bst6I CTCTTC 7 cut(s) 984, 1122, 1311, 1476, 1604, 2588, 2955
BstAPI GCANNNNNTGC 1 cut(s) 3293
BstAUI TGTACA 2 cut(s) 781, 1372
BstC8I GCNNGC 8 cut(s) 906, 1053, 2088, 2214, 2566, 2967, 3044, 3298
BstDSI CCRYGG 1 cut(s) 1619
BstEII GGTNACC 1 cut(s) 2886
BstH2I RGCGCY 1 cut(s) 3205
BstHHI GCGC 2 cut(s) 88, 3204
BstMAI GTCTC 3 cut(s) 1588, 2419, 2888
BstMWI GCNNNNNNNGC 5 cut(s) 371, 380, 389, 2459, 3293
BstNI CCWGG 8 cut(s) 579, 1068, 1166, 1629, 1775, 2196, 3055, 3425
BstNSI RCATGY 4 cut(s) 1009, 2193, 2216, 3562
BstPI GGTNACC 1 cut(s) 2886
BstSCI CCNGG 8 cut(s) 577, 1066, 1164, 1627, 1773, 2194, 3053, 3423
BstSFI CTRYAG 4 cut(s) 591, 921, 2586, 3417
BstV2I GAAGAC 1 cut(s) 2249
BstX2I RGATCY 3 cut(s) 952, 1588, 2624
BstXI CCANNNNNNTGG 3 cut(s) 2138, 2819, 2954
BstYI RGATCY 3 cut(s) 952, 1588, 2624
BsuI GTATCC 1 cut(s) 2561
BsuRI GGCC 4 cut(s) 1163, 1627, 2811, 3046
BtgI CCRYGG 1 cut(s) 1619
BtgZI GCGATG 1 cut(s) 3289
BtrI CACGTC 1 cut(s) 1847
BtsI GCAGTG 2 cut(s) 1793, 2053
BtsIMutI CAGTG 9 cut(s) 588, 621, 673, 1341, 1483, 1793, 2053, 2601, 3418
BveI ACCTGC 5 cut(s) 257, 767, 817, 886, 2620
Cac8I GCNNGC 8 cut(s) 906, 1053, 2088, 2214, 2566, 2967, 3044, 3298
CciI TCATGA 2 cut(s) 1012, 3463
CfoI GCGC 2 cut(s) 88, 3204
Cfr13I GGNCC 6 cut(s) 636, 1150, 1162, 1714, 2249, 3209
Csp6I GTAC 7 cut(s) 153, 539, 782, 1373, 1572, 2686, 2763
CspCI CAANNNNNGTGG 4 cut(s) 1330, 1365, 2833, 2868
CviQI GTAC 7 cut(s) 153, 539, 782, 1373, 1572, 2686, 2763
DraI TTTAAA 3 cut(s) 1326, 1749, 2103
EaeI YGGCCR 1 cut(s) 2809
Eam1104I CTCTTC 7 cut(s) 984, 1122, 1311, 1476, 1604, 2588, 2955
EarI CTCTTC 7 cut(s) 984, 1122, 1311, 1476, 1604, 2588, 2955
Ecl136II GAGCTC 2 cut(s) 1552, 3162
Eco130I CCWWGG 3 cut(s) 1619, 2073, 3007
Eco24I GRGCYC 2 cut(s) 1554, 3164
Eco47I GGWCC 5 cut(s) 636, 1150, 1714, 2249, 3209
Eco47III AGCGCT 1 cut(s) 3203
Eco53kI GAGCTC 2 cut(s) 1552, 3162
Eco57I CTGAAG 6 cut(s) 1413, 1536, 2762, 3156, 3236, 3662
Eco88I CYCGRG 1 cut(s) 45
Eco91I GGTNACC 1 cut(s) 2886
EcoICRI GAGCTC 2 cut(s) 1552, 3162
EcoO65I GGTNACC 1 cut(s) 2886
EcoRI GAATTC 4 cut(s) 622, 961, 2126, 2794
EcoRII CCWGG 8 cut(s) 577, 1066, 1164, 1627, 1773, 2194, 3053, 3423
EcoT14I CCWWGG 3 cut(s) 1619, 2073, 3007
EcoT22I ATGCAT 3 cut(s) 1076, 1877, 3474
EcoT38I GRGCYC 2 cut(s) 1554, 3164
ErhI CCWWGG 3 cut(s) 1619, 2073, 3007
Esp3I CGTCTC 1 cut(s) 2419
FalI AAGNNNNNCTT 2 cut(s) 3426, 3458
FaqI GGGAC 4 cut(s) 669, 883, 1727, 2195
FauI CCCGC 1 cut(s) 229
FbaI TGATCA 2 cut(s) 1249, 3460
FblI GTMKAC 3 cut(s) 1137, 2582, 2841
FriOI GRGCYC 2 cut(s) 1554, 3164
GlaI GCGC 2 cut(s) 87, 3203
GsaI CCCAGC 2 cut(s) 1929, 3312
GsuI CTGGAG 2 cut(s) 642, 3113
HaeII RGCGCY 1 cut(s) 3205
HaeIII GGCC 4 cut(s) 1163, 1627, 2811, 3046
HapII CCGG 3 cut(s) 639, 1759, 2252
HhaI GCGC 2 cut(s) 88, 3204
Hin6I GCGC 2 cut(s) 86, 3202
HinP1I GCGC 2 cut(s) 86, 3202
HincII GTYRAC 1 cut(s) 1213
HindII GTYRAC 1 cut(s) 1213
HindIII AAGCTT 1 cut(s) 2019
HpaI GTTAAC 1 cut(s) 1213
HpaII CCGG 3 cut(s) 639, 1759, 2252
HphI GGTGA 9 cut(s) 44, 272, 740, 938, 1241, 2210, 2511, 2555, 3169
Hpy166II GTNNAC 8 cut(s) 782, 1138, 1201, 1213, 1282, 1441, 2583, 2842
Hpy8I GTNNAC 8 cut(s) 782, 1138, 1201, 1213, 1282, 1441, 2583, 2842
HpyCH4III ACNGT 8 cut(s) 592, 616, 760, 889, 1691, 2031, 2500, 3340
HpyCH4IV ACGT 2 cut(s) 1846, 3395
HpyF10VI GCNNNNNNNGC 5 cut(s) 371, 380, 389, 2459, 3293
HpySE526I ACGT 2 cut(s) 1846, 3395
HspAI GCGC 2 cut(s) 86, 3202
Ksp22I TGATCA 2 cut(s) 1249, 3460
KspAI GTTAAC 1 cut(s) 1213
LguI GCTCTTC 3 cut(s) 1604, 2588, 2955
LmnI GCTCC 3 cut(s) 362, 661, 1557
MaeII ACGT 2 cut(s) 1846, 3395
MaeIII GTNAC 7 cut(s) 760, 937, 1336, 1387, 2048, 2709, 2886
MbiI CCGCTC 1 cut(s) 3634
MfeI CAATTG 1 cut(s) 1819
MflI RGATCY 3 cut(s) 952, 1588, 2624
MhlI GDGCHC 2 cut(s) 1554, 3164
MlsI TGGCCA 1 cut(s) 2811
MluNI TGGCCA 1 cut(s) 2811
MlyI GAGTC 6 cut(s) 67, 209, 296, 691, 848, 3085
MmeI TCCRAC 3 cut(s) 1009, 2130, 2373
Mox20I TGGCCA 1 cut(s) 2811
Mph1103I ATGCAT 3 cut(s) 1076, 1877, 3474
MscI TGGCCA 1 cut(s) 2811
MslI CAYNNNNRTG 3 cut(s) 1436, 1870, 3462
Msp20I TGGCCA 1 cut(s) 2811
MspA1I CMGCKG 1 cut(s) 374
MspI CCGG 3 cut(s) 639, 1759, 2252
MspR9I CCNGG 8 cut(s) 579, 1068, 1166, 1629, 1775, 2196, 3055, 3425
MssI GTTTAAAC 1 cut(s) 1326
MunI CAATTG 1 cut(s) 1819
Mva1269I GAATGC 2 cut(s) 238, 3127
MvaI CCWGG 8 cut(s) 579, 1068, 1166, 1629, 1775, 2196, 3055, 3425
MwoI GCNNNNNNNGC 5 cut(s) 371, 380, 389, 2459, 3293
NcoI CCATGG 1 cut(s) 1619
NlaIV GGNNCC 3 cut(s) 1715, 2033, 2371
NmuCI GTSAC 4 cut(s) 760, 1336, 2048, 2709
NsiI ATGCAT 3 cut(s) 1076, 1877, 3474
NspI RCATGY 4 cut(s) 1009, 2193, 2216, 3562
PaeI GCATGC 1 cut(s) 2216
PagI TCATGA 2 cut(s) 1012, 3463
PaqCI CACCTGC 1 cut(s) 767
PciI ACATGT 2 cut(s) 1005, 2189
PciSI GCTCTTC 3 cut(s) 1604, 2588, 2955
PctI GAATGC 2 cut(s) 238, 3127
PfeI GAWTC 5 cut(s) 574, 1905, 1940, 1967, 1991
PflMI CCANNNNNTGG 2 cut(s) 1159, 2417
PfoI TCCNGGA 2 cut(s) 577, 1066
PleI GAGTC 6 cut(s) 66, 208, 295, 690, 847, 3084
PmeI GTTTAAAC 1 cut(s) 1326
PpsI GAGTC 6 cut(s) 66, 208, 295, 690, 847, 3084
PscI ACATGT 2 cut(s) 1005, 2189
PshBI ATTAAT 1 cut(s) 2351
Psp124BI GAGCTC 2 cut(s) 1554, 3164
Psp6I CCWGG 8 cut(s) 577, 1066, 1164, 1627, 1773, 2194, 3053, 3423
PspEI GGTNACC 1 cut(s) 2886
PspFI CCCAGC 2 cut(s) 1925, 3308
PspGI CCWGG 8 cut(s) 577, 1066, 1164, 1627, 1773, 2194, 3053, 3423
PspN4I GGNNCC 3 cut(s) 1715, 2033, 2371
PspPI GGNCC 6 cut(s) 636, 1150, 1162, 1714, 2249, 3209
PstI CTGCAG 1 cut(s) 3421
PsuI RGATCY 3 cut(s) 952, 1588, 2624
PvuII CAGCTG 1 cut(s) 374
RsaI GTAC 7 cut(s) 154, 540, 783, 1374, 1573, 2687, 2764
RsaNI GTAC 7 cut(s) 153, 539, 782, 1373, 1572, 2686, 2763
RseI CAYNNNNRTG 3 cut(s) 1436, 1870, 3462
SacI GAGCTC 2 cut(s) 1554, 3164
SapI GCTCTTC 3 cut(s) 1604, 2588, 2955
Sau96I GGNCC 6 cut(s) 636, 1150, 1162, 1714, 2249, 3209
ScaI AGTACT 2 cut(s) 2687, 2764
SchI GAGTC 6 cut(s) 67, 209, 296, 691, 848, 3085
ScrFI CCNGG 8 cut(s) 579, 1068, 1166, 1629, 1775, 2196, 3055, 3425
SduI GDGCHC 2 cut(s) 1554, 3164
SfcI CTRYAG 4 cut(s) 591, 921, 2586, 3417
SinI GGWCC 5 cut(s) 636, 1150, 1714, 2249, 3209
SmiMI CAYNNNNRTG 3 cut(s) 1436, 1870, 3462
SmlI CTYRAG 4 cut(s) 2267, 2758, 3030, 3069
SmoI CTYRAG 4 cut(s) 2267, 2758, 3030, 3069
SpeI ACTAGT 1 cut(s) 155
SphI GCATGC 1 cut(s) 2216
SsiI CCGC 5 cut(s) 236, 317, 726, 2043, 3634
SstI GAGCTC 2 cut(s) 1554, 3164
StyD4I CCNGG 8 cut(s) 577, 1066, 1164, 1627, 1773, 2194, 3053, 3423
StyI CCWWGG 3 cut(s) 1619, 2073, 3007
TaaI ACNGT 8 cut(s) 592, 616, 760, 889, 1691, 2031, 2500, 3340
TaiI ACGT 2 cut(s) 1849, 3398
TaqI TCGA 2 cut(s) 1081, 1943
TaqII GACCGA 2 cut(s) 1731, 2709
TatI WGTACW 5 cut(s) 152, 781, 1372, 2685, 2762
TauI GCSGC 1 cut(s) 2046
TfiI GAWTC 5 cut(s) 574, 1905, 1940, 1967, 1991
TscAI CASTG 9 cut(s) 595, 621, 673, 1348, 1483, 1793, 2053, 2608, 3418
TseFI GTSAC 4 cut(s) 760, 1336, 2048, 2709
Tsp45I GTSAC 4 cut(s) 760, 1336, 2048, 2709
TspGWI ACGGA 5 cut(s) 472, 500, 758, 2443, 2989
TspRI CASTG 9 cut(s) 595, 621, 673, 1348, 1483, 1793, 2053, 2608, 3418
Van91I CCANNNNNTGG 2 cut(s) 1159, 2417
VpaK11BI GGWCC 5 cut(s) 636, 1150, 1714, 2249, 3209
VspI ATTAAT 1 cut(s) 2351
XapI RAATTY 8 cut(s) 622, 961, 1096, 2126, 2794, 2872, 2903, 3314
XbaI TCTAGA 1 cut(s) 2695
XceI RCATGY 4 cut(s) 1009, 2193, 2216, 3562
XmiI GTMKAC 3 cut(s) 1137, 2582, 2841
ZrmI AGTACT 2 cut(s) 2687, 2764
Zsp2I ATGCAT 3 cut(s) 1076, 1877, 3474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.