Rh1DG316100

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0003478
Physical Location & Seq
Reverse (-)
54240841 .. 54249382
8542 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG316100.1

Sequence Viewer

Length: 3525 bp
ATGGACGAAAGCAAAGACAAGGGTGTTGCTGGTGAAATTGTAGCTGGGGCTGTTGGGAGTCAGGGTCAGGTTAGTGAGGAAGGTGGACCTTGTAATGGGGATGATCTAATGGTGGAGGTTTTCGGGTCTAATGTGTATGTGGGTGGAGTTTGTACTAGTGGGGGAGGGGATGAGGAGGATAAGGAGGTGGGTCGTGAGGAGTCGGTTGAGGTTGATATGAGTTCGGGAAGGAATGCGGGGTCTGTGGGTGGAGATGGTGGTGATGCGGGGGCAGCTGCTGTTGCTGGTTCGGCTGGTGGAGAAACACAGGTAGTTGATGTTGAGAAAGCTGAAAAAGAAAGGAAACAAATTGGAGGACTTCTGGAGGAAGTTTTTGGGCGGGGGAATACGGATGGTGTGAAACAAGAAAAGAAGGTAGTTGATGGTGGGGCTGATTTGAAGGTACAGAAAGTTAGTGTTGCAGATGATGAAGTAAGGAATCCTGGAATTGACACTGTAGCTACAAGTTCTTCGGAAACAGTGGAGAATTCTTGTGTTCGGACGGAAAAAGCTCCAGTTGTCAGTGAAGAAGGTTCGAGTCCCAAACCCTTGAATGGACAAAGGCAAGTATCTGCTGGTGGTGGTGATGTTTCAGGCACGGACAAAGGAGATACTGTGACTTCCGAAGTTGCAGGTGTAGAGATTGGTAAAATTGATGAAAGTAATTCAGTAGGAAAACAGCAGGTTAAGATTGAGTCGCTAGGTGGAAACATCCATACTCACATCAATCTACCTGCACATACAGTTGCTTCGTCCCTGCCAGCTAAAGTAGTTCGTGTAGGTGAAATTGCTGTAACAGATGGAGAGGATCTCTTGAATTCTGAAAAAGATCATTTGAAATTAGAAGAGAGTGTAGAGAACATGTTTCATGATATTGCTCAGGTTGGATCAAATACTTCATCTCTGCCAGCCAAACTTCATCCTGGATGCATAGATCGAGAGGGTCTTTTGAATTTTGAAAGTCATCGGCACTTGAAACCCGAAGAGATTGTGGACAAGAACATGGTCCATGATATGGCCCAGGTTGAATTAAATGGAGAACAAGAAATGGAAGTGGACAACCAAGTTAATGATGCTGAACATGGTGAGTTAGTTTCAGGAAATGATCAGAATTCAAAAATTGAGAAATCATGCATGAACTCTGTTGGGAAGGATCAAAAGTTGAAGTTTGAAGAGAGTTTAAACAATAGTGTGACCACTGACCATGCTCAGTCCAAGACCAATGTTGTACATGAGGTTGAGGTAACTGAAGCTGATATGGATGGTTTATATGGAGAAAGCCTAGACATGGAAGTGGACAGAGATGCTAGTCATAGGGAACAACCAAAACCCAGTGAAGAGAAAATTGTTAATCAAGAAGCTATGCAGCCTGAAAGCTCTGAAATAGTGCCTCAGCTTAGATATGAGCTGCCACAAAAAAGCGAAGGTACATTTTCTGTCTCAGATTTAGTTTGGGGTAAAGTGAAGAGCCATCCATGGTGGCCTGGACAGATATTTGATTTTATGGCTGCATCTGAGAAGGCAATGAAACATCATAAGAAGGACTGTTTTTTGGTAGCATATTTTGGGGACCGAACATTTGCTTGGAATGAAGTATCTAGTTTAAAGCCTTTCCGATCTTATTTCTCCCAGGCAGAGAAGCAGTGCAATTCAGAAACATTCCACAAAGCTGTCAATTGTGTTCTGGAAGAAGTTTCAAGACGTGTAGAATTGGGTCTATCATGCTCATGCATACCAAAAGATGTTTATGAAAAGATCAGATTCCAGATTATTGAAAATGCTGGGATATGCCATGAATCTAGTAGAGTGGACGGAGTAGATGAATCTGCAAGTGCAAGTTCATTTGAATCTGATAAACTACTTAAATATGTAAAAGCTTTAGCACGGTTCCCATCTGGCGGCAGTGACAAATTAGAACTTGTCATTGCCAAGGCTCATTTGCTGGCATATTTTCGTTTAAAGGGTTATTGTAGTTTGCCTGAATTCCAGTTTTGTGGAGATTTGTTGGAGAGTAAAACAGATAATTCGCTCTCTGGGGACAAAACATGTCCGGGTGAAATTACCGAGCATGCGACTTATATTGGGAAGGATGATAAGAAGACTGGACCGGAGATTGAGGAACTCAAGTCTAGCACTTCTCATAAACGCAAACATAATCTAAGAGAAGGAGCTTATGCTAAAATGAAAGAAAGAAGCCTGTCTGAATTAATGGGTGGCGAAACAGGTTCTCCAGATGGTAATGATTGGTTGGATGCGAAGGCTTCGCCATCATCTGGCAAGAGACGGAAGGGTGCTGAGTATCAGGCTGCTGATTTAGCCACACAAGATGGGAGGAAAGCTGTTTCCCATTCAACAGTTTCAAACACAACACCTTCACCAAAACCATCGTTTAAGATAGGTGAATGTATCCAGAGAGCTGCAAGCCAACTATCAGGGTCTACTATAGTGAAGAGCAGCACTGACAGATCTGCTGTGCAGGGATCTGATGTTCCCTTCCAGAGTTCTGATGATGCTCTTAGAGGAGTGAATAATACAACAAAGTACTCATCTCTAGATGAATTGCTGTCACAACTTCGGTCAGCAGCAGAAGAACCTCTGAAGGAATACAATTCCTTGAGTACTATTGTCAATTTCTTCTCTGATTTTAGGAATTCTGTGGTTGTTGGCCAGAAATCTGGGGTGGTGCTTTCAGTTGTAGACAAAGTTGGTGGTAAAAAGAGGAAGTCAAATTCTGTGCATGGGTTACCTGAGACTTTTGAATTTGATGATATGAATGACACTTATTGGACAGACATGGTAATCCAAAATGGTGGAGAAGAGCAAGCATCACGGAAAAGAAAAACGAAGTATCAACCTGTCGTCCTTGGGCAACCAGAAAAGCCCCCTCAAGTGGGTCGCAGGCCATACACCAGGAAGAAGTTTTCTCAAGGGAGTCAAGATTTGCCTCCGGAGAAACCTGTTGGCTATGTGGATGAGAATGCACCAGCAGAACTTGTAATGAGCTTCTCTGAGGTGAGCTCTATTCCTTCAGAAACAAACCTGAATAAAATGTTCAAGCGCTTTGGACCTCTGAAGGAATATGAAACAGAAGTTGATAGGGAGAGCAGCCGTGCTAGAGTTGTTTTTAAGAGATGTTCTGATGCAGAAGTTGCTTGCAATAGTGCTGGGAAATTCAACATCTTTGGACAGATCACTGTAAATTACCAGCTCAACTATACTCCCTCTCAGCTCAACTACACGCCATCTATTACGTTCGGTGCATCTACCAGTGCTGCAGCCCAGGATCAGGAAGTGCAACTTGTTTTCCCCTCCCATGATCATGAGATGCATCTTGATCTCTCCACCCATGACCAGATGCAGCTTGATCTTTCCACCCATGACCAGATGCAGCTTGACCTTTCCACCCATGACCACATGCAACTTGATCTTTCAACCCATGACGAGATGCAACTAGACCTCTCAACATTTGAAGTTAATCTGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

1174

Amino Acids

127.91

Weight (kDa)

4.97

Isoelectric Point (pI)

39.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 495 - 577 4.1e-17 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 662
Acc36I ACCTGC 3 cut(s) 662, 712, 781
AccB7I CCANNNNNTGG 2 cut(s) 1054, 2312
AccI GTMKAC 2 cut(s) 2477, 2736
AccIII TCCGGA 1 cut(s) 2986
AciI CCGC 4 cut(s) 236, 266, 379, 1938
AclWI GGATC 5 cut(s) 855, 934, 1200, 2527, 3330
AcoI YGGCCR 1 cut(s) 2704
AcsI RAATTY 9 cut(s) 526, 856, 991, 1150, 2021, 2689, 2767, 2798, 3209
AcuI CTGAAG 4 cut(s) 1308, 2657, 3051, 3131
AfaI GTAC 6 cut(s) 154, 444, 1269, 1468, 2582, 2659
AfeI AGCGCT 1 cut(s) 3098
AfiI CCNNNNNNNGG 9 cut(s) 95, 593, 1054, 1327, 1937, 2312, 2929, 2930, 3325
AflIII ACRYGT 3 cut(s) 900, 1741, 2084
AhlI ACTAGT 1 cut(s) 155
AjiI CACGTC 1 cut(s) 1742
AjnI CCWGG 7 cut(s) 481, 961, 1059, 1522, 1668, 2948, 3318
AjuI GAANNNNNNNTTGG 2 cut(s) 1606, 1638
AloI GAACNNNNNNTCC 2 cut(s) 2511, 2543
Alw21I GWGCWC 1 cut(s) 3059
Alw26I GTCTC 3 cut(s) 1483, 2314, 2783
AlwI GGATC 5 cut(s) 855, 934, 1200, 2527, 3330
AlwNI CAGNNNCTG 1 cut(s) 278
Aor13HI TCCGGA 1 cut(s) 2986
Aor51HI AGCGCT 1 cut(s) 3098
AoxI GGCC 4 cut(s) 1056, 1520, 2704, 2939
ApoI RAATTY 9 cut(s) 526, 856, 991, 1150, 2021, 2689, 2767, 2798, 3209
ArsI GACNNNNNNTTYG 2 cut(s) 1601, 1633
AseI ATTAAT 1 cut(s) 2246
AspLEI GCGC 1 cut(s) 3099
AspS9I GGNCC 6 cut(s) 86, 1045, 1057, 1609, 2144, 3104
AsuC2I CCSGG 1 cut(s) 2091
AsuHPI GGTGA 9 cut(s) 44, 272, 635, 833, 1136, 2105, 2406, 2450, 3064
AvaII GGWCC 5 cut(s) 86, 1045, 1609, 2144, 3104
BalI TGGCCA 1 cut(s) 2706
BanII GRGCYC 1 cut(s) 3059
BbsI GAAGAC 1 cut(s) 2144
Bbv12I GWGCWC 1 cut(s) 3059
BbvCI CCTCAGC 1 cut(s) 1431
BceAI ACGGC 1 cut(s) 3132
BciT130I CCWGG 7 cut(s) 483, 963, 1061, 1524, 1670, 2950, 3320
BciVI GTATCC 1 cut(s) 2456
BclI TGATCA 2 cut(s) 1144, 3355
BcnI CCSGG 1 cut(s) 2091
BcoDI GTCTC 3 cut(s) 1483, 2314, 2783
BcuI ACTAGT 1 cut(s) 155
BfmI CTRYAG 3 cut(s) 495, 2481, 3312
BfoI RGCGCY 1 cut(s) 3100
BfuAI ACCTGC 3 cut(s) 662, 712, 781
BfuI GTATCC 1 cut(s) 2456
BglII AGATCT 1 cut(s) 2504
BmcAI AGTACT 2 cut(s) 2582, 2659
Bme1390I CCNGG 8 cut(s) 483, 963, 1061, 1524, 1670, 2091, 2950, 3320
Bme18I GGWCC 5 cut(s) 86, 1045, 1609, 2144, 3104
BmgBI CACGTC 1 cut(s) 1742
BmgT120I GGNCC 6 cut(s) 86, 1045, 1057, 1609, 2144, 3104
BmiI GGNNCC 2 cut(s) 1610, 1928
BmrFI CCNGG 8 cut(s) 483, 963, 1061, 1524, 1670, 2091, 2950, 3320
BmrI ACTGGG 1 cut(s) 1365
BmuI ACTGGG 1 cut(s) 1365
BpiI GAAGAC 1 cut(s) 2144
BplI GAGNNNNNCTC 4 cut(s) 834, 866, 3041, 3073
BpmI CTGGAG 3 cut(s) 383, 537, 2253
Bpu10I CCTNAGC 2 cut(s) 918, 1431
BpuEI CTTGAG 4 cut(s) 2147, 2674, 2910, 2949
BpuMI CCSGG 1 cut(s) 2091
BsaJI CCNNGG 6 cut(s) 1059, 1514, 1668, 1968, 2902, 3318
BsaWI WCCGGW 2 cut(s) 2146, 2986
BsaXI ACNNNNNCTCC 6 cut(s) 49, 79, 639, 669, 2251, 2281
Bsc4I CCNNNNNNNGG 9 cut(s) 95, 593, 1054, 1327, 1937, 2312, 2929, 2930, 3325
Bse1I ACTGG 5 cut(s) 554, 1371, 2026, 2146, 3306
Bse3DI GCAATG 2 cut(s) 1569, 1962
BseAI TCCGGA 1 cut(s) 2986
BseBI CCWGG 7 cut(s) 483, 963, 1061, 1524, 1670, 2950, 3320
BseDI CCNNGG 6 cut(s) 1059, 1514, 1668, 1968, 2902, 3318
BseLI CCNNNNNNNGG 9 cut(s) 95, 593, 1054, 1327, 1937, 2312, 2929, 2930, 3325
BseMI GCAATG 2 cut(s) 1569, 1962
BseMII CTCAG 9 cut(s) 932, 1262, 1445, 1494, 1545, 2325, 2778, 3039, 3278
BseNI ACTGG 5 cut(s) 554, 1371, 2026, 2146, 3306
BseRI GAGGAG 3 cut(s) 188, 212, 2574
BseYI CCCAGC 3 cut(s) 44, 1820, 3203
BsgI GTGCAG 2 cut(s) 759, 2534
BshFI GGCC 4 cut(s) 1058, 1522, 2706, 2941
BsiHKAI GWGCWC 1 cut(s) 3059
BsiSI CCGG 3 cut(s) 2090, 2147, 2987
BslFI GGGAC 4 cut(s) 564, 778, 1622, 2090
BslI CCNNNNNNNGG 9 cut(s) 95, 593, 1054, 1327, 1937, 2312, 2929, 2930, 3325
BsmAI GTCTC 3 cut(s) 1483, 2314, 2783
BsmBI CGTCTC 1 cut(s) 2314
BsmFI GGGAC 4 cut(s) 564, 778, 1622, 2090
BsmI GAATGC 2 cut(s) 238, 3022
BsnI GGCC 4 cut(s) 1058, 1522, 2706, 2941
Bsp1286I GDGCHC 1 cut(s) 3059
Bsp13I TCCGGA 1 cut(s) 2986
Bsp1407I TGTACA 1 cut(s) 1267
Bsp19I CCATGG 1 cut(s) 1514
BspACI CCGC 4 cut(s) 236, 266, 379, 1938
BspANI GGCC 4 cut(s) 1058, 1522, 2706, 2941
BspCNI CTCAG 9 cut(s) 931, 1261, 1444, 1493, 1546, 2326, 2779, 3040, 3277
BspEI TCCGGA 1 cut(s) 2986
BspHI TCATGA 2 cut(s) 907, 3358
BspLI GGNNCC 2 cut(s) 1610, 1928
BspMAI CTGCAG 1 cut(s) 3316
BspMI ACCTGC 3 cut(s) 662, 712, 781
BspPI GGATC 5 cut(s) 855, 934, 1200, 2527, 3330
BspQI GCTCTTC 3 cut(s) 1499, 2483, 2850
BsrDI GCAATG 2 cut(s) 1569, 1962
BsrGI TGTACA 1 cut(s) 1267
BsrI ACTGG 5 cut(s) 554, 1371, 2026, 2146, 3306
BssECI CCNNGG 6 cut(s) 1059, 1514, 1668, 1968, 2902, 3318
BssT1I CCWWGG 3 cut(s) 1514, 1968, 2902
Bst2UI CCWGG 7 cut(s) 483, 963, 1061, 1524, 1670, 2950, 3320
Bst4CI ACNGT 8 cut(s) 496, 520, 655, 784, 1586, 1926, 2395, 3235
Bst6I CTCTTC 7 cut(s) 879, 1017, 1206, 1371, 1499, 2483, 2850
BstAPI GCANNNNNTGC 1 cut(s) 3188
BstAUI TGTACA 1 cut(s) 1267
BstC8I GCNNGC 8 cut(s) 801, 948, 1983, 2109, 2461, 2862, 2939, 3193
BstDSI CCRYGG 1 cut(s) 1514
BstEII GGTNACC 1 cut(s) 2781
BstH2I RGCGCY 1 cut(s) 3100
BstHHI GCGC 1 cut(s) 3099
BstMAI GTCTC 3 cut(s) 1483, 2314, 2783
BstMWI GCNNNNNNNGC 5 cut(s) 272, 281, 290, 2354, 3188
BstNI CCWGG 7 cut(s) 483, 963, 1061, 1524, 1670, 2950, 3320
BstNSI RCATGY 4 cut(s) 904, 2088, 2111, 3457
BstPI GGTNACC 1 cut(s) 2781
BstSCI CCNGG 8 cut(s) 481, 961, 1059, 1522, 1668, 2089, 2948, 3318
BstSFI CTRYAG 3 cut(s) 495, 2481, 3312
BstV2I GAAGAC 1 cut(s) 2144
BstX2I RGATCY 3 cut(s) 847, 2504, 2519
BstXI CCANNNNNNTGG 3 cut(s) 2033, 2714, 2849
BstYI RGATCY 3 cut(s) 847, 2504, 2519
BsuI GTATCC 1 cut(s) 2456
BsuRI GGCC 4 cut(s) 1058, 1522, 2706, 2941
BtgI CCRYGG 1 cut(s) 1514
BtrI CACGTC 1 cut(s) 1742
BtsI GCAGTG 2 cut(s) 1688, 1948
BveI ACCTGC 3 cut(s) 662, 712, 781
Cac8I GCNNGC 8 cut(s) 801, 948, 1983, 2109, 2461, 2862, 2939, 3193
CaiI CAGNNNCTG 1 cut(s) 278
CciI TCATGA 2 cut(s) 907, 3358
CfoI GCGC 1 cut(s) 3099
Cfr13I GGNCC 6 cut(s) 86, 1045, 1057, 1609, 2144, 3104
Csp6I GTAC 6 cut(s) 153, 443, 1268, 1467, 2581, 2658
CspCI CAANNNNNGTGG 2 cut(s) 2728, 2763
CviQI GTAC 6 cut(s) 153, 443, 1268, 1467, 2581, 2658
DraI TTTAAA 3 cut(s) 1221, 1644, 1998
EaeI YGGCCR 1 cut(s) 2704
Eam1104I CTCTTC 7 cut(s) 879, 1017, 1206, 1371, 1499, 2483, 2850
EarI CTCTTC 7 cut(s) 879, 1017, 1206, 1371, 1499, 2483, 2850
Ecl136II GAGCTC 1 cut(s) 3057
Eco130I CCWWGG 3 cut(s) 1514, 1968, 2902
Eco24I GRGCYC 1 cut(s) 3059
Eco47I GGWCC 5 cut(s) 86, 1045, 1609, 2144, 3104
Eco47III AGCGCT 1 cut(s) 3098
Eco53kI GAGCTC 1 cut(s) 3057
Eco57I CTGAAG 4 cut(s) 1308, 2657, 3051, 3131
Eco91I GGTNACC 1 cut(s) 2781
EcoICRI GAGCTC 1 cut(s) 3057
EcoO65I GGTNACC 1 cut(s) 2781
EcoRI GAATTC 5 cut(s) 526, 856, 1150, 2021, 2689
EcoRII CCWGG 7 cut(s) 481, 961, 1059, 1522, 1668, 2948, 3318
EcoT14I CCWWGG 3 cut(s) 1514, 1968, 2902
EcoT22I ATGCAT 4 cut(s) 971, 1175, 1772, 3369
EcoT38I GRGCYC 1 cut(s) 3059
ErhI CCWWGG 3 cut(s) 1514, 1968, 2902
Esp3I CGTCTC 1 cut(s) 2314
FalI AAGNNNNNCTT 2 cut(s) 3321, 3353
FaqI GGGAC 4 cut(s) 564, 778, 1622, 2090
FauI CCCGC 3 cut(s) 229, 259, 372
FbaI TGATCA 2 cut(s) 1144, 3355
FblI GTMKAC 2 cut(s) 2477, 2736
FriOI GRGCYC 1 cut(s) 3059
GlaI GCGC 1 cut(s) 3098
GsaI CCCAGC 3 cut(s) 48, 1824, 3207
GsuI CTGGAG 3 cut(s) 383, 537, 2253
HaeII RGCGCY 1 cut(s) 3100
HaeIII GGCC 4 cut(s) 1058, 1522, 2706, 2941
HapII CCGG 3 cut(s) 2090, 2147, 2987
HhaI GCGC 1 cut(s) 3099
Hin6I GCGC 1 cut(s) 3097
HinP1I GCGC 1 cut(s) 3097
HindIII AAGCTT 1 cut(s) 1914
HpaII CCGG 3 cut(s) 2090, 2147, 2987
HphI GGTGA 9 cut(s) 44, 272, 635, 833, 1136, 2105, 2406, 2450, 3064
Hpy166II GTNNAC 7 cut(s) 86, 1033, 1096, 1336, 1849, 2478, 2737
Hpy8I GTNNAC 7 cut(s) 86, 1033, 1096, 1336, 1849, 2478, 2737
HpyCH4III ACNGT 8 cut(s) 496, 520, 655, 784, 1586, 1926, 2395, 3235
HpyCH4IV ACGT 2 cut(s) 1741, 3290
HpyF10VI GCNNNNNNNGC 5 cut(s) 272, 281, 290, 2354, 3188
HpySE526I ACGT 2 cut(s) 1741, 3290
HspAI GCGC 1 cut(s) 3097
Kpn2I TCCGGA 1 cut(s) 2986
Ksp22I TGATCA 2 cut(s) 1144, 3355
LguI GCTCTTC 3 cut(s) 1499, 2483, 2850
LmnI GCTCC 2 cut(s) 556, 2207
MaeII ACGT 2 cut(s) 1741, 3290
MaeIII GTNAC 7 cut(s) 655, 832, 1231, 1282, 1943, 2604, 2781
MfeI CAATTG 1 cut(s) 1714
MflI RGATCY 3 cut(s) 847, 2504, 2519
MhlI GDGCHC 1 cut(s) 3059
MlsI TGGCCA 1 cut(s) 2706
MluNI TGGCCA 1 cut(s) 2706
MlyI GAGTC 5 cut(s) 67, 209, 586, 743, 2980
MmeI TCCRAC 3 cut(s) 904, 2025, 2268
Mox20I TGGCCA 1 cut(s) 2706
Mph1103I ATGCAT 4 cut(s) 971, 1175, 1772, 3369
MroI TCCGGA 1 cut(s) 2986
MscI TGGCCA 1 cut(s) 2706
MslI CAYNNNNRTG 3 cut(s) 1331, 1765, 3357
Msp20I TGGCCA 1 cut(s) 2706
MspA1I CMGCKG 1 cut(s) 275
MspI CCGG 3 cut(s) 2090, 2147, 2987
MspR9I CCNGG 8 cut(s) 483, 963, 1061, 1524, 1670, 2091, 2950, 3320
MssI GTTTAAAC 1 cut(s) 1221
MunI CAATTG 1 cut(s) 1714
Mva1269I GAATGC 2 cut(s) 238, 3022
MvaI CCWGG 7 cut(s) 483, 963, 1061, 1524, 1670, 2950, 3320
MwoI GCNNNNNNNGC 5 cut(s) 272, 281, 290, 2354, 3188
NciI CCSGG 1 cut(s) 2091
NcoI CCATGG 1 cut(s) 1514
NlaIV GGNNCC 2 cut(s) 1610, 1928
NmuCI GTSAC 4 cut(s) 655, 1231, 1943, 2604
NsiI ATGCAT 4 cut(s) 971, 1175, 1772, 3369
NspI RCATGY 4 cut(s) 904, 2088, 2111, 3457
PaeI GCATGC 1 cut(s) 2111
PagI TCATGA 2 cut(s) 907, 3358
PaqCI CACCTGC 1 cut(s) 662
PciI ACATGT 2 cut(s) 900, 2084
PciSI GCTCTTC 3 cut(s) 1499, 2483, 2850
PctI GAATGC 2 cut(s) 238, 3022
PfeI GAWTC 5 cut(s) 478, 1800, 1835, 1862, 1886
PflMI CCANNNNNTGG 2 cut(s) 1054, 2312
PfoI TCCNGGA 2 cut(s) 481, 961
PleI GAGTC 5 cut(s) 66, 208, 585, 742, 2979
PmeI GTTTAAAC 1 cut(s) 1221
PpsI GAGTC 5 cut(s) 66, 208, 585, 742, 2979
PscI ACATGT 2 cut(s) 900, 2084
PshBI ATTAAT 1 cut(s) 2246
Psp124BI GAGCTC 1 cut(s) 3059
Psp6I CCWGG 7 cut(s) 481, 961, 1059, 1522, 1668, 2948, 3318
PspEI GGTNACC 1 cut(s) 2781
PspFI CCCAGC 3 cut(s) 44, 1820, 3203
PspGI CCWGG 7 cut(s) 481, 961, 1059, 1522, 1668, 2948, 3318
PspN4I GGNNCC 2 cut(s) 1610, 1928
PspPI GGNCC 6 cut(s) 86, 1045, 1057, 1609, 2144, 3104
PstI CTGCAG 1 cut(s) 3316
PstNI CAGNNNCTG 1 cut(s) 278
PsuI RGATCY 3 cut(s) 847, 2504, 2519
PvuII CAGCTG 1 cut(s) 275
RsaI GTAC 6 cut(s) 154, 444, 1269, 1468, 2582, 2659
RsaNI GTAC 6 cut(s) 153, 443, 1268, 1467, 2581, 2658
RseI CAYNNNNRTG 3 cut(s) 1331, 1765, 3357
SacI GAGCTC 1 cut(s) 3059
SapI GCTCTTC 3 cut(s) 1499, 2483, 2850
Sau96I GGNCC 6 cut(s) 86, 1045, 1057, 1609, 2144, 3104
ScaI AGTACT 2 cut(s) 2582, 2659
SchI GAGTC 5 cut(s) 67, 209, 586, 743, 2980
ScrFI CCNGG 8 cut(s) 483, 963, 1061, 1524, 1670, 2091, 2950, 3320
SduI GDGCHC 1 cut(s) 3059
SfcI CTRYAG 3 cut(s) 495, 2481, 3312
SinI GGWCC 5 cut(s) 86, 1045, 1609, 2144, 3104
SmiMI CAYNNNNRTG 3 cut(s) 1331, 1765, 3357
SmlI CTYRAG 4 cut(s) 2162, 2653, 2925, 2964
SmoI CTYRAG 4 cut(s) 2162, 2653, 2925, 2964
SpeI ACTAGT 1 cut(s) 155
SphI GCATGC 1 cut(s) 2111
SsiI CCGC 4 cut(s) 236, 266, 379, 1938
SstI GAGCTC 1 cut(s) 3059
StyD4I CCNGG 8 cut(s) 481, 961, 1059, 1522, 1668, 2089, 2948, 3318
StyI CCWWGG 3 cut(s) 1514, 1968, 2902
TaaI ACNGT 8 cut(s) 496, 520, 655, 784, 1586, 1926, 2395, 3235
TaiI ACGT 2 cut(s) 1744, 3293
TaqI TCGA 2 cut(s) 575, 976
TaqII GACCGA 2 cut(s) 1626, 2604
TatI WGTACW 4 cut(s) 152, 1267, 2580, 2657
TauI GCSGC 1 cut(s) 1941
TfiI GAWTC 5 cut(s) 478, 1800, 1835, 1862, 1886
TseFI GTSAC 4 cut(s) 655, 1231, 1943, 2604
Tsp45I GTSAC 4 cut(s) 655, 1231, 1943, 2604
TspGWI ACGGA 6 cut(s) 404, 557, 653, 1866, 2338, 2884
Van91I CCANNNNNTGG 2 cut(s) 1054, 2312
VpaK11BI GGWCC 5 cut(s) 86, 1045, 1609, 2144, 3104
VspI ATTAAT 1 cut(s) 2246
XapI RAATTY 9 cut(s) 526, 856, 991, 1150, 2021, 2689, 2767, 2798, 3209
XbaI TCTAGA 1 cut(s) 2590
XceI RCATGY 4 cut(s) 904, 2088, 2111, 3457
XmiI GTMKAC 2 cut(s) 2477, 2736
ZrmI AGTACT 2 cut(s) 2582, 2659
Zsp2I ATGCAT 4 cut(s) 971, 1175, 1772, 3369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.