Rw1G028520

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0003478
Physical Location & Seq
Reverse (-)
56175004 .. 56178893
3890 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G028520.1

Sequence Viewer

Length: 3585 bp
ATGGACGAAAGCAAAGACAAGGGTGTTGCTGGTGAAATTGTAGCTGGGGCTGTTGGGAGTCAGGGTCAGGTTAGTGAGGAAGGTGCGCCTTGTAATGGGGATGATCTAATGGTGGAGGTTTTCGGGTCTAATGTGTATGTGGGTGGAGTTTGTACTAGTGGGGGAGGGGATGAGGAGGATAAGGAGCTGGGTCATGAGGAGTCGGTTGAGGTTGATATGGGTTCGGGAAGGAATGCGGGGTCTGTGGGTGGAGATGGTGGTGAAGCGTGTTCGGGTGGGAAGGTAGAGTCGGTGGAAGAAAGAAGGGTAGGGGTTGCGGTGGAGGAGGTTGGGGTGGTGGTTAGGGAGGAGAGTTCAAAGGAAGCAGGGGCCGCTGCTGCTGCTGGTTCAGCTGGTGGAGAAACACAGGTAGTTGATGTTGAGAAAGCTGAAAAAGAAAGGAAACAAATTGGAGGACTTCTGGAGGAAGATTTTGGGTGTGGGATTACGGATGGTCTGAAACAAGAAAAGAAGGTAGTTGATGGTGGGGCTGATTTGAAGGTACAGAAAGTTAGTGTTGCAGATGATGAAGTAAGGAATCCTGGAATTGACACTGTAGCTGCAAGTTCTTCGGAAACAGTGGAGAATTCTTGTGTTCGGACCCGAGTTGTGGAAAGAGCTCCAGTTGTCAGTGAAGAAGGTTCGAGTCCCAAACCCTTGAATGGACGAAGGCAAGTATCTGCTGGTGGTGGTGATGGTTCAGGCACGGACAAAGGAGATACTGTGACTTCCGAAGTTGCAGGTGTAGAGATTGGTAAAATTGATGAAATTAATTCAGTAGGAAAACAGCAGGTTAAGATTGAGCCGCTAGGTGGAAACATCCATACTCACATCAATCTACCTGCACATACAGTTGCTTCGTCCCTGCCAGCTAACGGAGAGGATCTCTTGAATTCTGAAAAAGATCATTTGAGATTAGAAGAGAGTGTAGAGAACATGTTTCATGATATTGCTCAGGTTGGATCAAATACTTCATCTCTGCCAGCAAAACTACATCCTGGATGCATAGATCGAGAGGGTGTTTTGAATTTTGAAAGTCATCAGCACTTGAAACCTGAAGAGATTGTAGACAAGAACATGGTCCATGATATGGCCCAGGTTGAATTAAATGGAGGGCAAGAAATGGAAGTGGACAGCCAAGTTAATGATGCTGAACATGGTGAGTTAGTTTCAGGAAATGATCAGAACTCAAAAATTGAGAAATCATGCATGAACTCTGTTGGGAAGGATCAAAAGTTGAAGTTTGAAGAGAGTTTAAACAATAATGTGACCACTGTTCTTGCTCAGTCCAAGACCAATGTTGTAGATGAGGTTGAGGTAACTGAAGCTGATATGGATGGTTTATATGGAGAAAGCCTAGACATGGAAGTGGACAGAGATGCTAGTCATAGGGAACAACCAAAACCCAGTGAAGAGAAAATTGTTATTCAAGAAGCTACGCAGCCTGAAAGCTCTGAAATAGTGCCTCAACTTAGATATGAGCTGCCACAAAAAAATGAAGGTACATTTTCTGTCTCAGATTTAGTTTGGGGTAAAGTGAAGAGCCATCCATGGTGGCCTGGACAGATATTTGATTTTATGGCTGCATCTGAGAAGGCAATGAAACATCATAAGAAGGACTGTTTTTTGGTGGCATATTTTGGGGACCGAACATTTGCATGGAATGAAGTATCTAGTTTAAAGCCTTTCCGATCTTATTTCTCCCAGGCAGAGAAGCAGTGCAATTCAGAAACATTCCACAAAGCTGTCAATTGTGTTCTGGAAGAAGTTTCAAGACGAGTAGAATTGGGTCTATCATGCTCATGCATACCAAAAGATGTTTATGAAAAGATCAGATTCCAGATTATTGAAAATGCTGGGATATGCCATGAATCGAGTAGAGTGGAAGGAGTAGATGAATCTGCAGGTGCAAGTTCATTTGAATCTGATAAACTACTTAAATATGTAAAAGCTTTAGCACGGTTCCCATCTGGTGGCAGTGACAAATTAGAACTTGTCATTGCGAAGGCTCATTTGCTGGCATTTTTCCGTTTAAAGGGTTATTGTAGTTTGCCTGAATTCCAGTTTTGTGGAGATTTGTTGGAAAGTAAAACAGATAATTCGCTCTCTGGGGACAAAACATGTTCAGGTGAAATTACTGAGCATGCGACTTATATTGGGAAGGATGATAAGAAGACTGGCCCGGAGATTGAGGAACTCAAGTCTAGCTCTTCTCATAAACGTAAACATAATCTAAGAGAAGGAGTGTATGCTAAAATGAAAGAAAGAAGCCTGTCAGAATTAATGGGTGGTGAAACAGGTTCTCCAGATGGTAATGATTGGTTGGATGTGAAGGCTTCGCCATTATCTGGCAAGAGACGGAAGGGTGCCGAGTATCTGGCTGCTGATTTAGCCACGCAAGATGGGAGGAAAGCTGTTTCCCATTCAACAGTTTCACCAAAACCATCGTTTAAGATTGGTGAATGTATCCAGAGAGCTGCAAGCCAACTGTCAGGTTCTACTATAGTGAAGAGCAGCACTGACAGACCTGCTGTGCAGGGATCTGATGTCCCCTTCCAGAATTCCGATGATACTCTCAGAGGAGTGAATAATACAACGAAGTACTCATCTCTAGATGAATTGCTGTCACAACTTCGGTCAGCAGCAGAAGAACCTCTGAAGGAATACAATTCCTTGAGTACTATTGTCAATTTCTTCTCTGATTTTAGGAATTCTGTGGTTGTTGGCCAGAAATGTGGGGTGGTGCTTTCAGTTGTAGACAAAGTCGGTGGTAAAAAGAGGAAGTCAAATTCTGTGCATGGGTTACCTGAGACTTTTGAATTTGATGATATGAATGACACTTATTGGACAGACATGGTAATCCAAAATGGTGGAGAAGAGCAACCATCACGGAAAAGAAAAACGAAGTATCAACCTGTCGTCCTTGGGCAACCAGAAAAGCCCCCTCAAGTGGGTCGCAGGCCATACACCAGGAAGAAGTTTTCTCAAGGGAGTCAAGATTTGCCTCCGGAGAAACCTGTTGGCTATGTGGATGAGAATGCACCAGCAGAACTTGTAATGAGCTTCTCTGAGGTGAGCTCTATTCCTTCAGAAACAAACCTGAATAAAATGTTCAAGCGCTTTGGACCTCTGAAGGAATATGAAACAGAAGTTGATAGGGAGAGCAGCCGTGCTAGAGTTGTTTTTAAGAGATGTTCTGATGCAGAAGTTGCTTGCAATAGTGCTGGGAAATTCAACATCTTTGGACAGATTACTGTAAATTACCAGCTCAACTATACTCCCTCTCAGCTCAACTACACGCCATCTATTACGTTTGGTGCATCTACCAGTGCTGCAGCCCAGGATCAGGAGGTGCAACTTGTTTTTCCCTCCCATGATCATGAGATGCATCTTGATCTCTCCACCCATGACCAGATGCAGCTTGATCTTTCCACCCATGACCAGATGCAGCTTGACCTTTCCACCCATGACCACATGCAGCTTGATCTTTCAACCCATGACGAGATGCAACTAGACCTCTCAACATTCGAAGTTAATCTGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

1194

Amino Acids

130.02

Weight (kDa)

4.93

Isoelectric Point (pI)

40.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 520 - 602 4.2e-17 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 770, 1934
Acc36I ACCTGC 5 cut(s) 770, 820, 889, 1934, 2575
AccB1I GGYRCC 1 cut(s) 2405
AccB7I CCANNNNNTGG 3 cut(s) 1129, 2012, 2387
AccI GTMKAC 2 cut(s) 1107, 2796
AccIII TCCGGA 1 cut(s) 3046
AciI CCGC 4 cut(s) 236, 317, 372, 845
AclWI GGATC 5 cut(s) 930, 1009, 1275, 2587, 3390
AcoI YGGCCR 1 cut(s) 2764
AcsI RAATTY 9 cut(s) 625, 931, 1066, 2096, 2599, 2749, 2827, 2858, 3269
AcuI CTGAAG 5 cut(s) 1116, 1383, 2717, 3111, 3191
AfaI GTAC 5 cut(s) 154, 543, 1543, 2642, 2719
AfeI AGCGCT 1 cut(s) 3158
AflIII ACRYGT 2 cut(s) 975, 2159
AhlI ACTAGT 1 cut(s) 155
AjnI CCWGG 7 cut(s) 580, 1036, 1134, 1597, 1743, 3008, 3378
Alw21I GWGCWC 2 cut(s) 661, 3119
Alw26I GTCTC 3 cut(s) 1558, 2389, 2843
AlwI GGATC 5 cut(s) 930, 1009, 1275, 2587, 3390
Ama87I CYCGRG 1 cut(s) 642
Aor13HI TCCGGA 1 cut(s) 3046
Aor51HI AGCGCT 1 cut(s) 3158
AoxI GGCC 6 cut(s) 369, 1131, 1595, 2218, 2764, 2999
ApoI RAATTY 9 cut(s) 625, 931, 1066, 2096, 2599, 2749, 2827, 2858, 3269
ArsI GACNNNNNNTTYG 2 cut(s) 1676, 1708
AseI ATTAAT 2 cut(s) 810, 2321
AspLEI GCGC 2 cut(s) 88, 3159
AspS9I GGNCC 7 cut(s) 369, 639, 1120, 1132, 1684, 2219, 3164
AsuC2I CCSGG 1 cut(s) 2222
AsuHPI GGTGA 9 cut(s) 44, 272, 743, 1211, 2180, 2342, 2466, 2510, 3124
AsuII TTCGAA 1 cut(s) 3567
AvaI CYCGRG 1 cut(s) 642
AvaII GGWCC 4 cut(s) 639, 1120, 1684, 3164
BalI TGGCCA 1 cut(s) 2766
BanI GGYRCC 1 cut(s) 2405
BanII GRGCYC 2 cut(s) 661, 3119
BbsI GAAGAC 1 cut(s) 2219
Bbv12I GWGCWC 2 cut(s) 661, 3119
BceAI ACGGC 1 cut(s) 3192
BcgI CGANNNNNNTGC 2 cut(s) 591, 625
BciT130I CCWGG 7 cut(s) 582, 1038, 1136, 1599, 1745, 3010, 3380
BciVI GTATCC 1 cut(s) 2516
BclI TGATCA 2 cut(s) 1219, 3415
BcnI CCSGG 1 cut(s) 2222
BcoDI GTCTC 3 cut(s) 1558, 2389, 2843
BcuI ACTAGT 1 cut(s) 155
BfaI CTAG 9 cut(s) 156, 848, 1397, 1422, 1713, 2244, 2651, 3213, 3551
BfmI CTRYAG 4 cut(s) 594, 1941, 2541, 3372
BfoI RGCGCY 1 cut(s) 3160
BfuAI ACCTGC 5 cut(s) 770, 820, 889, 1934, 2575
BfuI GTATCC 1 cut(s) 2516
BmcAI AGTACT 2 cut(s) 2642, 2719
Bme1390I CCNGG 8 cut(s) 582, 1038, 1136, 1599, 1745, 2222, 3010, 3380
Bme18I GGWCC 4 cut(s) 639, 1120, 1684, 3164
BmeT110I CYCGRG 1 cut(s) 642
BmgT120I GGNCC 7 cut(s) 369, 639, 1120, 1132, 1684, 2219, 3164
BmiI GGNNCC 5 cut(s) 370, 641, 1685, 2003, 2407
BmrFI CCNGG 8 cut(s) 582, 1038, 1136, 1599, 1745, 2222, 3010, 3380
BmrI ACTGGG 1 cut(s) 1440
BmuI ACTGGG 1 cut(s) 1440
BpiI GAAGAC 1 cut(s) 2219
BplI GAGNNNNNCTC 4 cut(s) 909, 941, 3101, 3133
BpmI CTGGAG 3 cut(s) 482, 645, 2328
Bpu10I CCTNAGC 1 cut(s) 993
Bpu14I TTCGAA 1 cut(s) 3567
BpuEI CTTGAG 4 cut(s) 2222, 2734, 2970, 3009
BpuMI CCSGG 1 cut(s) 2222
BsaJI CCNNGG 5 cut(s) 1134, 1589, 1743, 2962, 3378
BsaWI WCCGGW 1 cut(s) 3046
BsaXI ACNNNNNCTCC 6 cut(s) 49, 79, 747, 777, 2326, 2356
Bse1I ACTGG 5 cut(s) 662, 1446, 2101, 2221, 3366
Bse3DI GCAATG 2 cut(s) 1644, 2037
BseAI TCCGGA 1 cut(s) 3046
BseBI CCWGG 7 cut(s) 582, 1038, 1136, 1599, 1745, 3010, 3380
BseDI CCNNGG 5 cut(s) 1134, 1589, 1743, 2962, 3378
BseMI GCAATG 2 cut(s) 1644, 2037
BseMII CTCAG 9 cut(s) 1007, 1337, 1569, 1620, 2169, 2629, 2838, 3099, 3338
BseNI ACTGG 5 cut(s) 662, 1446, 2101, 2221, 3366
BseRI GAGGAG 5 cut(s) 188, 212, 338, 362, 2634
BseYI CCCAGC 4 cut(s) 44, 187, 1895, 3263
BsgI GTGCAG 2 cut(s) 867, 2594
BshFI GGCC 6 cut(s) 371, 1133, 1597, 2220, 2766, 3001
BshNI GGYRCC 1 cut(s) 2405
BsiHKAI GWGCWC 2 cut(s) 661, 3119
BsiHKCI CYCGRG 1 cut(s) 642
BsiSI CCGG 2 cut(s) 2222, 3047
BslFI GGGAC 5 cut(s) 672, 886, 1697, 2165, 2573
BsmAI GTCTC 3 cut(s) 1558, 2389, 2843
BsmBI CGTCTC 1 cut(s) 2389
BsmFI GGGAC 5 cut(s) 672, 886, 1697, 2165, 2573
BsmI GAATGC 2 cut(s) 238, 3082
BsnI GGCC 6 cut(s) 371, 1133, 1597, 2220, 2766, 3001
BsoBI CYCGRG 1 cut(s) 642
Bsp119I TTCGAA 1 cut(s) 3567
Bsp1286I GDGCHC 2 cut(s) 661, 3119
Bsp13I TCCGGA 1 cut(s) 3046
Bsp19I CCATGG 1 cut(s) 1589
BspACI CCGC 4 cut(s) 236, 317, 372, 845
BspANI GGCC 6 cut(s) 371, 1133, 1597, 2220, 2766, 3001
BspCNI CTCAG 9 cut(s) 1006, 1336, 1568, 1621, 2170, 2628, 2839, 3100, 3337
BspEI TCCGGA 1 cut(s) 3046
BspHI TCATGA 3 cut(s) 193, 982, 3418
BspLI GGNNCC 5 cut(s) 370, 641, 1685, 2003, 2407
BspMAI CTGCAG 2 cut(s) 1945, 3376
BspMI ACCTGC 5 cut(s) 770, 820, 889, 1934, 2575
BspPI GGATC 5 cut(s) 930, 1009, 1275, 2587, 3390
BspQI GCTCTTC 4 cut(s) 1574, 2254, 2543, 2910
BspT104I TTCGAA 1 cut(s) 3567
BspT107I GGYRCC 1 cut(s) 2405
BsrDI GCAATG 2 cut(s) 1644, 2037
BsrI ACTGG 5 cut(s) 662, 1446, 2101, 2221, 3366
BssECI CCNNGG 5 cut(s) 1134, 1589, 1743, 2962, 3378
BssT1I CCWWGG 2 cut(s) 1589, 2962
Bst2UI CCWGG 7 cut(s) 582, 1038, 1136, 1599, 1745, 3010, 3380
Bst6I CTCTTC 8 cut(s) 954, 1092, 1281, 1446, 1574, 2254, 2543, 2910
BstAPI GCANNNNNTGC 1 cut(s) 3248
BstBI TTCGAA 1 cut(s) 3567
BstC8I GCNNGC 7 cut(s) 909, 1023, 2058, 2184, 2521, 2999, 3253
BstDSI CCRYGG 1 cut(s) 1589
BstEII GGTNACC 1 cut(s) 2841
BstH2I RGCGCY 1 cut(s) 3160
BstHHI GCGC 2 cut(s) 88, 3159
BstMAI GTCTC 3 cut(s) 1558, 2389, 2843
BstMWI GCNNNNNNNGC 6 cut(s) 371, 377, 380, 389, 2429, 3248
BstNI CCWGG 7 cut(s) 582, 1038, 1136, 1599, 1745, 3010, 3380
BstNSI RCATGY 4 cut(s) 979, 2163, 2186, 3517
BstPI GGTNACC 1 cut(s) 2841
BstSCI CCNGG 8 cut(s) 580, 1036, 1134, 1597, 1743, 2220, 3008, 3378
BstSFI CTRYAG 4 cut(s) 594, 1941, 2541, 3372
BstV2I GAAGAC 1 cut(s) 2219
BstX2I RGATCY 2 cut(s) 922, 2579
BstXI CCANNNNNNTGG 3 cut(s) 2108, 2774, 2909
BstYI RGATCY 2 cut(s) 922, 2579
BsuI GTATCC 1 cut(s) 2516
BsuRI GGCC 6 cut(s) 371, 1133, 1597, 2220, 2766, 3001
BtgI CCRYGG 1 cut(s) 1589
BtsI GCAGTG 2 cut(s) 1763, 2023
BtsIMutI CAGTG 9 cut(s) 591, 624, 676, 1311, 1453, 1763, 2023, 2556, 3373
BveI ACCTGC 5 cut(s) 770, 820, 889, 1934, 2575
Cac8I GCNNGC 7 cut(s) 909, 1023, 2058, 2184, 2521, 2999, 3253
CciI TCATGA 3 cut(s) 193, 982, 3418
CfoI GCGC 2 cut(s) 88, 3159
Cfr13I GGNCC 7 cut(s) 369, 639, 1120, 1132, 1684, 2219, 3164
Csp6I GTAC 5 cut(s) 153, 542, 1542, 2641, 2718
CspCI CAANNNNNGTGG 4 cut(s) 1300, 1335, 2788, 2823
CviQI GTAC 5 cut(s) 153, 542, 1542, 2641, 2718
DraI TTTAAA 3 cut(s) 1296, 1719, 2073
EaeI YGGCCR 1 cut(s) 2764
Eam1104I CTCTTC 8 cut(s) 954, 1092, 1281, 1446, 1574, 2254, 2543, 2910
EarI CTCTTC 8 cut(s) 954, 1092, 1281, 1446, 1574, 2254, 2543, 2910
Ecl136II GAGCTC 2 cut(s) 659, 3117
Eco130I CCWWGG 2 cut(s) 1589, 2962
Eco24I GRGCYC 2 cut(s) 661, 3119
Eco47I GGWCC 4 cut(s) 639, 1120, 1684, 3164
Eco47III AGCGCT 1 cut(s) 3158
Eco53kI GAGCTC 2 cut(s) 659, 3117
Eco57I CTGAAG 5 cut(s) 1116, 1383, 2717, 3111, 3191
Eco88I CYCGRG 1 cut(s) 642
Eco91I GGTNACC 1 cut(s) 2841
EcoICRI GAGCTC 2 cut(s) 659, 3117
EcoO65I GGTNACC 1 cut(s) 2841
EcoRI GAATTC 5 cut(s) 625, 931, 2096, 2599, 2749
EcoRII CCWGG 7 cut(s) 580, 1036, 1134, 1597, 1743, 3008, 3378
EcoT14I CCWWGG 2 cut(s) 1589, 2962
EcoT22I ATGCAT 4 cut(s) 1046, 1250, 1847, 3429
EcoT38I GRGCYC 2 cut(s) 661, 3119
ErhI CCWWGG 2 cut(s) 1589, 2962
Esp3I CGTCTC 1 cut(s) 2389
FaqI GGGAC 5 cut(s) 672, 886, 1697, 2165, 2573
FauI CCCGC 1 cut(s) 229
FbaI TGATCA 2 cut(s) 1219, 3415
FblI GTMKAC 2 cut(s) 1107, 2796
FriOI GRGCYC 2 cut(s) 661, 3119
FspBI CTAG 9 cut(s) 156, 848, 1397, 1422, 1713, 2244, 2651, 3213, 3551
GlaI GCGC 2 cut(s) 87, 3158
GsaI CCCAGC 4 cut(s) 48, 191, 1899, 3267
GsuI CTGGAG 3 cut(s) 482, 645, 2328
HaeII RGCGCY 1 cut(s) 3160
HaeIII GGCC 6 cut(s) 371, 1133, 1597, 2220, 2766, 3001
HapII CCGG 2 cut(s) 2222, 3047
HhaI GCGC 2 cut(s) 88, 3159
Hin6I GCGC 2 cut(s) 86, 3157
HinP1I GCGC 2 cut(s) 86, 3157
HindIII AAGCTT 1 cut(s) 1989
HpaII CCGG 2 cut(s) 2222, 3047
HphI GGTGA 9 cut(s) 44, 272, 743, 1211, 2180, 2342, 2466, 2510, 3124
Hpy166II GTNNAC 5 cut(s) 1108, 1171, 1411, 2264, 2797
Hpy8I GTNNAC 5 cut(s) 1108, 1171, 1411, 2264, 2797
HpyCH4IV ACGT 2 cut(s) 2260, 3350
HpyF10VI GCNNNNNNNGC 6 cut(s) 371, 377, 380, 389, 2429, 3248
HpySE526I ACGT 2 cut(s) 2260, 3350
HspAI GCGC 2 cut(s) 86, 3157
Kpn2I TCCGGA 1 cut(s) 3046
Ksp22I TGATCA 2 cut(s) 1219, 3415
LguI GCTCTTC 4 cut(s) 1574, 2254, 2543, 2910
LmnI GCTCC 2 cut(s) 184, 664
MaeI CTAG 9 cut(s) 156, 848, 1397, 1422, 1713, 2244, 2651, 3213, 3551
MaeII ACGT 2 cut(s) 2260, 3350
MaeIII GTNAC 6 cut(s) 763, 1306, 1357, 2018, 2664, 2841
MfeI CAATTG 1 cut(s) 1789
MflI RGATCY 2 cut(s) 922, 2579
MhlI GDGCHC 2 cut(s) 661, 3119
MlsI TGGCCA 1 cut(s) 2766
MluNI TGGCCA 1 cut(s) 2766
MlyI GAGTC 5 cut(s) 67, 209, 296, 694, 3040
MmeI TCCRAC 3 cut(s) 979, 2100, 2343
Mox20I TGGCCA 1 cut(s) 2766
Mph1103I ATGCAT 4 cut(s) 1046, 1250, 1847, 3429
MroI TCCGGA 1 cut(s) 3046
MscI TGGCCA 1 cut(s) 2766
MslI CAYNNNNRTG 4 cut(s) 1406, 1696, 1840, 3417
Msp20I TGGCCA 1 cut(s) 2766
MspA1I CMGCKG 2 cut(s) 374, 392
MspI CCGG 2 cut(s) 2222, 3047
MspR9I CCNGG 8 cut(s) 582, 1038, 1136, 1599, 1745, 2222, 3010, 3380
MssI GTTTAAAC 1 cut(s) 1296
MunI CAATTG 1 cut(s) 1789
Mva1269I GAATGC 2 cut(s) 238, 3082
MvaI CCWGG 7 cut(s) 582, 1038, 1136, 1599, 1745, 3010, 3380
MwoI GCNNNNNNNGC 6 cut(s) 371, 377, 380, 389, 2429, 3248
NciI CCSGG 1 cut(s) 2222
NcoI CCATGG 1 cut(s) 1589
NlaIV GGNNCC 5 cut(s) 370, 641, 1685, 2003, 2407
NmeAIII GCCGAG 1 cut(s) 2434
NmuCI GTSAC 4 cut(s) 763, 1306, 2018, 2664
NsiI ATGCAT 4 cut(s) 1046, 1250, 1847, 3429
NspI RCATGY 4 cut(s) 979, 2163, 2186, 3517
NspV TTCGAA 1 cut(s) 3567
PaeI GCATGC 1 cut(s) 2186
PagI TCATGA 3 cut(s) 193, 982, 3418
PaqCI CACCTGC 2 cut(s) 770, 1934
PciI ACATGT 2 cut(s) 975, 2159
PciSI GCTCTTC 4 cut(s) 1574, 2254, 2543, 2910
PctI GAATGC 2 cut(s) 238, 3082
PfeI GAWTC 5 cut(s) 577, 1875, 1910, 1937, 1961
PflFI GACNNNGTC 1 cut(s) 2801
PflMI CCANNNNNTGG 3 cut(s) 1129, 2012, 2387
PfoI TCCNGGA 2 cut(s) 580, 1036
PleI GAGTC 5 cut(s) 66, 208, 295, 693, 3039
PmeI GTTTAAAC 1 cut(s) 1296
PpsI GAGTC 5 cut(s) 66, 208, 295, 693, 3039
PscI ACATGT 2 cut(s) 975, 2159
PshBI ATTAAT 2 cut(s) 810, 2321
Psp124BI GAGCTC 2 cut(s) 661, 3119
Psp6I CCWGG 7 cut(s) 580, 1036, 1134, 1597, 1743, 3008, 3378
PspEI GGTNACC 1 cut(s) 2841
PspFI CCCAGC 4 cut(s) 44, 187, 1895, 3263
PspGI CCWGG 7 cut(s) 580, 1036, 1134, 1597, 1743, 3008, 3378
PspN4I GGNNCC 5 cut(s) 370, 641, 1685, 2003, 2407
PspPI GGNCC 7 cut(s) 369, 639, 1120, 1132, 1684, 2219, 3164
PstI CTGCAG 2 cut(s) 1945, 3376
PsuI RGATCY 2 cut(s) 922, 2579
PsyI GACNNNGTC 1 cut(s) 2801
PvuII CAGCTG 1 cut(s) 392
RsaI GTAC 5 cut(s) 154, 543, 1543, 2642, 2719
RsaNI GTAC 5 cut(s) 153, 542, 1542, 2641, 2718
RseI CAYNNNNRTG 4 cut(s) 1406, 1696, 1840, 3417
SacI GAGCTC 2 cut(s) 661, 3119
SapI GCTCTTC 4 cut(s) 1574, 2254, 2543, 2910
Sau96I GGNCC 7 cut(s) 369, 639, 1120, 1132, 1684, 2219, 3164
ScaI AGTACT 2 cut(s) 2642, 2719
SchI GAGTC 5 cut(s) 67, 209, 296, 694, 3040
ScrFI CCNGG 8 cut(s) 582, 1038, 1136, 1599, 1745, 2222, 3010, 3380
SduI GDGCHC 2 cut(s) 661, 3119
SfcI CTRYAG 4 cut(s) 594, 1941, 2541, 3372
SfuI TTCGAA 1 cut(s) 3567
SinI GGWCC 4 cut(s) 639, 1120, 1684, 3164
SmiMI CAYNNNNRTG 4 cut(s) 1406, 1696, 1840, 3417
SmlI CTYRAG 4 cut(s) 2237, 2713, 2985, 3024
SmoI CTYRAG 4 cut(s) 2237, 2713, 2985, 3024
SpeI ACTAGT 1 cut(s) 155
SphI GCATGC 1 cut(s) 2186
SsiI CCGC 4 cut(s) 236, 317, 372, 845
SspMI CTAG 9 cut(s) 156, 848, 1397, 1422, 1713, 2244, 2651, 3213, 3551
SstI GAGCTC 2 cut(s) 661, 3119
StyD4I CCNGG 8 cut(s) 580, 1036, 1134, 1597, 1743, 2220, 3008, 3378
StyI CCWWGG 2 cut(s) 1589, 2962
TaiI ACGT 2 cut(s) 2263, 3353
TaqI TCGA 4 cut(s) 683, 1051, 1913, 3567
TaqII GACCGA 2 cut(s) 1701, 2664
TatI WGTACW 3 cut(s) 152, 2640, 2717
TauI GCSGC 2 cut(s) 374, 847
TfiI GAWTC 5 cut(s) 577, 1875, 1910, 1937, 1961
TscAI CASTG 9 cut(s) 598, 624, 676, 1318, 1453, 1763, 2023, 2563, 3373
TseFI GTSAC 4 cut(s) 763, 1306, 2018, 2664
Tsp45I GTSAC 4 cut(s) 763, 1306, 2018, 2664
TspGWI ACGGA 6 cut(s) 503, 761, 930, 2057, 2413, 2944
TspRI CASTG 9 cut(s) 598, 624, 676, 1318, 1453, 1763, 2023, 2563, 3373
Tth111I GACNNNGTC 1 cut(s) 2801
Van91I CCANNNNNTGG 3 cut(s) 1129, 2012, 2387
VpaK11BI GGWCC 4 cut(s) 639, 1120, 1684, 3164
VspI ATTAAT 2 cut(s) 810, 2321
XapI RAATTY 9 cut(s) 625, 931, 1066, 2096, 2599, 2749, 2827, 2858, 3269
XbaI TCTAGA 1 cut(s) 2650
XceI RCATGY 4 cut(s) 979, 2163, 2186, 3517
XmiI GTMKAC 2 cut(s) 1107, 2796
XspI CTAG 9 cut(s) 156, 848, 1397, 1422, 1713, 2244, 2651, 3213, 3551
ZrmI AGTACT 2 cut(s) 2642, 2719
Zsp2I ATGCAT 4 cut(s) 1046, 1250, 1847, 3429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.