MD07G1179800.v1.1

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003478
Physical Location & Seq
Reverse (-)
25919282 .. 25924443
5162 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1179800.v1.1.491

Sequence Viewer

Length: 3657 bp
ATGGTGGAGGTTCTGGGTTCTAATGTCTATGTTGGTGGAGTTTGCACTAGTGGGGATGGGGACAATGAAGGGGTGGGTCATGATGATTCGGTTGAGGTTGATATGGGGTTGGAGGGCAATGTGAGATCTCCTGGAGGAGATGGTGGTGAAATGGGTGAGCTGGATTCGGTGGCTGCAGTTGTTAGTTCAACGAGCATAGAAACCCAGGTTTTTCATTTTGAGGAAGCTGAGATGGTTGTGAGGCAGGATGTGAATTCAGAGGAAGTTAGTGTTTCGGATGAAAAATCACAGGATGCCACAGTAGAAAATGGAATTGAGGGTTCCTTGATTGGATCTGTAGGTAGTGAAACACAAGTTGTCGTCAGTGAAGTAGTTGTGAAGGAAAGTGTAGAAGTTTCCGTGGGAGGATTGGTGGAGGTTGTGGAACAAGAAAGGAAGTCGGTTGTTGGTGATGTTGATGCACTCAATGATTTGAAGGCACAAATAGCTGGTGTTTCAGATGATGTAGTATTGAATCCTGGAATTGAAATGGCTGCAGTGGCTGTTAGTGAAGAAGGTTTGAATCCCGAACCCTCGAGTGAACAAACCCAAGTTTCTGCTGCTAGTGGTGATGTTGCTGGGTTGGACAATGTAGATATTTTGACTTCCAAAGTTGCAGGTAAGGAAACTGATAAAACTAATGAAAATTCAAGTCATCCAGTAGAAGAACGGCTGGTTAAGATTGAGCCAGTGGGTAGTTGCACCCGTAGTGGCAGCAATGAACCTGAATATTCAGTGTCTTCTCCTATGCCGCCTCAAGTAGTTCATGGAAGTGACATGGGAGCAACAGGTGCACAAGATTCTGAAAATTATCAGTTCTTGAAACCTGAAGAAAGCGTAGAGGACATGGTTCATGATATTGCATTGGTTTCAACTACCTTATCTCCGCCAGCTCAAGTAATTCCTGGAGGTGTAGTTTCAGTAACAGAAAGAGGGGGCCTTTTGAATTCCAAAAAGGACCTGCACTTGAAACCTGAAGAGAGTATAGGCAAGAACACGGTCCTTGATACTGCACTGGTTGAATCAAATACAGGGCAAGAGATGGAAGTGGACACACACGTTAATGGTGCTGAACAGGATAGCGTAGATGGAACAGATCAATCCCTGGAGTCTGGAAAGTGTTTAGAAAAGACTGCGACAGCTTATGTTGATCGAGTTCATCCTGATATCGAAGCGGAGGAAGTCAATGAACAAGTGACTGATGCTGAGGGTGTATTAAATGGAGGGGAACAGATAGAAGATGAAGGCCAAGACATAATGGGTGGATCCACTGAGATTGCAGCAGCAGATGATAATATTCGTCTGAATCCATGCATTGAAGTAAATAACTCTATTGATCCGAACTTGAAAAGTGAGACTGTTCAGAGGAGATCACAAGCTGATATGCAAGTCACTGATAGTGGAGATGTTACTCCCATGGACACTGAGGAGGTAGGAGTTAAAGAACAATTTAATGATGCTGTGCTAGATGGTTTACACGGAGGGCAATACATGGAAGGCGAAAAAGAAGCTACTGATGCTGAACAACCAAAAGCAGGTGAAGAGGAAATTCTTAAACAAGAAGCTATCCAGGGAAGCTCAGAAATTGTGCCTCAACCGAGATATGAGCTGCCACCAGAAAATCATGTGTTTTCTGCATCTGATTTAGTTTGGGGTAAAGTGAAGAGTCATCCATGGTGGCCTGGACAGATATTTGATTATACGGTTGCATCTGAGAAGGCAATGAAATATCATAAAAAGGATTGCTTTTTGGTAGCATATTTTGGGGACCGTACTTTTGCTTGGAATGAACCATGTAGTTTAAAGCCTTTTCGATCTTACTTTTCCCAGTCAGAGAATCAATGTATTTCAGAAGCATTCCAGAATGCTGTCAATTGTGCTCTGGAAGAAGTTTCGAGACGTGTTGAATTGGGGCTAGCATGCTCTTGCATACCAATAGAAGTCTATGAGAAGATTAGGTTCCAGAAAGTTGAAAATGCCGGGATTCGCCAAGAATCAAGTAGAAGAGATGAAGTGGATGAATCGGCAAGTGCCAGTTCTCTTGAATGTAATCAATTGCTGGATTACATAAAAGCTTTAGCACGGTTCCCATCTGTTAACTGTGACCGATTAGAAGTTGTGATAGCTAAGGCTCATGTGCTATCATTTTATCGCTTGAAGGGTTACTACAACTTGCCAGAATTCCAATCTTGCGGAGATATATTGGAAAACAGAGATGAAAGGATTGAGCTTACAACTCCTAATGGCAAGGAAGCTAAAGGTACTCTCTCTGGCCCTGAGATTATTACCTATTCTCACAAGCGTAAACATAGTTTAAGAGATTCTAAGGTAAAAGAACGAAGCCTGTCTGAATTAATGGATGGCGGCTTAGATTCTCTGGATGGCGGTGATTGGTTGGATGGGAAAGATACTGGTGGTTTGGTCTCACCATCCTCTGGCAAGAGACGGAAGGGTTTTGAGTATCATGTTGATGATGATGGAAGGAAAGTTAGCGGAGCAAAAGTTTCTAATACCACGCCTGTCCCAAAACAGTCTTTTAAGATTGGTGAATGTATCCAGAGAGTTGCAAGCCAACTTACAGGGTCTCCTATTGTGAAGTCCAACAGTGACAGACCTGCTGGGGATGGATCTGATGTTGCCTTCCAGAGTTCTGGTGATGTCCACAGAGGGAAAGCAGTTGATAGATCAGAGTACTTGTCGTTAGATGAATTGCTGTCACAACTTCAGTTGGCAGCAGAAGATCCACAGAAGGATAACCTAAGCTTGAAAACTGTTGTTAATTTCTTCTCTGATTTTAGGAATTCTGTAGCTGTAGGTCAGCTTCCTGGGATAGAGCATTTAGCTGTGGACAAAGTTGGTGGGAGAAAGAGGAAGTCGTCAAATTCTGCTTTTGGATTGCCTGAAACTTTTGAATTTGATGACATGAATGATACTTATTGGACAGACAGGGTAATCCAAAATGGCGTTGAAGAGCAAACACCGCGAAGGGGCAGGAAGGCTAATTATCAACCCATTGTTCTTGCCCAACCAGAAAAATCCCCTCAAGAGGGTCGTAGGCCATACGCCAGGAAGCGGTATTCCCAAACTGTTAATGCTTTGCCGCCAGAGAAGCCTGTTGGCTATGTGGATGAGAATGCCCCAACAGAACTTGTTATGAACTTCTCTGAGGTGAATTCTGTTCCCTCAGAAACAAAGCTGAATAGAATGTTTAGGCGCTTTGGACCTCTGAAGGAAGCTGAAACAGAAGTTGACAGGGAGAGCAGTCGTGCTAGAGTTGTTTTCAAGAAATGTTCTGATGCAGAGGTTGCTATTGATAGTGCTGGAAAATTCAAGATCTTTGGATCAATACCTGTAAATTACCAGCTCAACTATACTCCATCTCAGCTCAACTATACTCCGTCTATCCAGTTTAGCATTTCTCCCAGTGCCACAGCGCATGATCAGGAGATGCAACTTGTTCTCCCCTCCCATGATCATGATATGCACCTTGATCTCTCCGCCCATGACCAGATGCAACTTGATCTTTCCACCCACGACCATATGCAACTGGATATGCAACTTGATCTTTCCTCTCATGACCAGATGCACCTTGATCTCTCCACCTTTGATAACCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

1219

Amino Acids

132.95

Weight (kDa)

4.57

Isoelectric Point (pI)

45.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 561 - 644 7.6e-17 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1565
Acc36I ACCTGC 4 cut(s) 647, 1008, 1565, 2662
AccB7I CCANNNNNTGG 1 cut(s) 2474
AccII CGCG 1 cut(s) 3022
AclWI GGATC 7 cut(s) 340, 1299, 1312, 1370, 2674, 2774, 3388
AcuI CTGAAG 4 cut(s) 888, 1035, 2747, 3287
AfaI GTAC 3 cut(s) 1813, 2302, 2732
AfiI CCNNNNNNNGG 6 cut(s) 1574, 2474, 3026, 3085, 3086, 3111
AflIII ACRYGT 2 cut(s) 1096, 1939
AhlI ACTAGT 1 cut(s) 47
AjiI CACGTC 1 cut(s) 1940
AjnI CCWGG 9 cut(s) 130, 204, 517, 943, 1143, 1608, 1720, 2863, 3104
Alw21I GWGCWC 2 cut(s) 835, 1921
Alw26I GTCTC 5 cut(s) 1388, 1930, 2467, 2476, 2628
Alw44I GTGCAC 1 cut(s) 831
AlwI GGATC 7 cut(s) 340, 1299, 1312, 1370, 2674, 2774, 3388
AlwNI CAGNNNCTG 1 cut(s) 542
Ama87I CYCGRG 1 cut(s) 574
AoxI GGCC 5 cut(s) 976, 1285, 1718, 2311, 3095
ApaLI GTGCAC 1 cut(s) 831
ApeKI GCWGC 8 cut(s) 173, 533, 599, 753, 1319, 1322, 1648, 2771
ArsI GACNNNNNNTTYG 2 cut(s) 1799, 1831
AseI ATTAAT 1 cut(s) 2393
AspLEI GCGC 2 cut(s) 3255, 3475
AspS9I GGNCC 6 cut(s) 976, 997, 1039, 1807, 2312, 3260
AsuC2I CCSGG 1 cut(s) 2020
AsuNHI GCTAGC 1 cut(s) 1954
AvaI CYCGRG 1 cut(s) 574
AvaII GGWCC 4 cut(s) 997, 1039, 1807, 3260
BaeGI GKGCMC 1 cut(s) 835
BamHI GGATCC 1 cut(s) 1304
BarI GAAGNNNNNNTAC 4 cut(s) 2189, 2221, 2844, 2876
BbsI GAAGAC 1 cut(s) 771
Bbv12I GWGCWC 2 cut(s) 835, 1921
BbvCI CCTCAGC 1 cut(s) 1245
BbvI GCAGC 8 cut(s) 160, 520, 586, 765, 1331, 1334, 1635, 2783
BceAI ACGGC 1 cut(s) 725
BciT130I CCWGG 9 cut(s) 132, 206, 519, 945, 1145, 1610, 1722, 2865, 3106
BciVI GTATCC 1 cut(s) 2603
BclI TGATCA 2 cut(s) 3478, 3511
BcnI CCSGG 1 cut(s) 2020
BcoDI GTCTC 5 cut(s) 1388, 1930, 2467, 2476, 2628
BcuI ACTAGT 1 cut(s) 47
BfaI CTAG 5 cut(s) 48, 603, 1505, 1955, 3309
BfmI CTRYAG 5 cut(s) 174, 336, 534, 2844, 2850
BfoI RGCGCY 1 cut(s) 3256
BfuAI ACCTGC 4 cut(s) 647, 1008, 1565, 2662
BfuI GTATCC 1 cut(s) 2603
BglII AGATCT 2 cut(s) 125, 3372
BmcAI AGTACT 1 cut(s) 2732
Bme18I GGWCC 4 cut(s) 997, 1039, 1807, 3260
BmeT110I CYCGRG 1 cut(s) 574
BmgBI CACGTC 1 cut(s) 1940
BmgT120I GGNCC 6 cut(s) 976, 997, 1039, 1807, 2312, 3260
BmiI GGNNCC 6 cut(s) 322, 977, 1306, 1808, 2000, 2126
BmrI ACTGGG 2 cut(s) 1861, 3456
BmtI GCTAGC 1 cut(s) 1958
BmuI ACTGGG 2 cut(s) 1861, 3456
BpiI GAAGAC 1 cut(s) 771
BpmI CTGGAG 3 cut(s) 153, 966, 1166
Bpu10I CCTNAGC 3 cut(s) 1245, 2166, 2798
BpuEI CTTGAG 3 cut(s) 780, 918, 3066
BpuMI CCSGG 1 cut(s) 2020
BsaI GGTCTC 2 cut(s) 2467, 2628
BsaJI CCNNGG 7 cut(s) 204, 399, 1143, 1455, 1609, 1712, 2864
Bsc4I CCNNNNNNNGG 6 cut(s) 1574, 2474, 3026, 3085, 3086, 3111
Bse1I ACTGG 9 cut(s) 698, 728, 1059, 1867, 2073, 2455, 3445, 3462, 3591
Bse3DI GCAATG 3 cut(s) 124, 763, 1767
BseBI CCWGG 9 cut(s) 132, 206, 519, 945, 1145, 1610, 1722, 2865, 3106
BseDI CCNNGG 7 cut(s) 204, 399, 1143, 1455, 1609, 1712, 2864
BseLI CCNNNNNNNGG 6 cut(s) 1574, 2474, 3026, 3085, 3086, 3111
BseMI GCAATG 3 cut(s) 124, 763, 1767
BseNI ACTGG 9 cut(s) 698, 728, 1059, 1867, 2073, 2455, 3445, 3462, 3591
BseRI GAGGAG 3 cut(s) 150, 1420, 1481
BseSI GKGCMC 1 cut(s) 835
BseXI GCAGC 8 cut(s) 160, 520, 586, 765, 1331, 1334, 1635, 2783
BseYI CCCAGC 2 cut(s) 617, 2657
BsgI GTGCAG 2 cut(s) 986, 1035
Bsh1236I CGCG 1 cut(s) 3022
BshFI GGCC 5 cut(s) 978, 1287, 1720, 2313, 3097
BsiHKAI GWGCWC 2 cut(s) 835, 1921
BsiHKCI CYCGRG 1 cut(s) 574
BsiSI CCGG 1 cut(s) 2019
BslFI GGGAC 3 cut(s) 74, 1820, 2546
BslI CCNNNNNNNGG 6 cut(s) 1574, 2474, 3026, 3085, 3086, 3111
BsmAI GTCTC 5 cut(s) 1388, 1930, 2467, 2476, 2628
BsmBI CGTCTC 2 cut(s) 1930, 2476
BsmFI GGGAC 3 cut(s) 74, 1820, 2546
BsmI GAATGC 3 cut(s) 1895, 1909, 3178
BsnI GGCC 5 cut(s) 978, 1287, 1720, 2313, 3097
Bso31I GGTCTC 2 cut(s) 2467, 2628
BsoBI CYCGRG 1 cut(s) 574
Bsp1286I GDGCHC 2 cut(s) 835, 1921
Bsp19I CCATGG 2 cut(s) 1455, 1712
BspANI GGCC 5 cut(s) 978, 1287, 1720, 2313, 3097
BspFNI CGCG 1 cut(s) 3022
BspHI TCATGA 4 cut(s) 79, 892, 3514, 3613
BspLI GGNNCC 6 cut(s) 322, 977, 1306, 1808, 2000, 2126
BspMAI CTGCAG 2 cut(s) 178, 538
BspMI ACCTGC 4 cut(s) 647, 1008, 1565, 2662
BspOI GCTAGC 1 cut(s) 1958
BspPI GGATC 7 cut(s) 340, 1299, 1312, 1370, 2674, 2774, 3388
BspQI GCTCTTC 1 cut(s) 3003
BspTNI GGTCTC 2 cut(s) 2467, 2628
BsrDI GCAATG 3 cut(s) 124, 763, 1767
BsrI ACTGG 9 cut(s) 698, 728, 1059, 1867, 2073, 2455, 3445, 3462, 3591
BssECI CCNNGG 7 cut(s) 204, 399, 1143, 1455, 1609, 1712, 2864
BssT1I CCWWGG 2 cut(s) 1455, 1712
Bst2UI CCWGG 9 cut(s) 132, 206, 519, 945, 1145, 1610, 1722, 2865, 3106
Bst6I CTCTTC 5 cut(s) 1011, 1575, 1697, 2038, 3003
BstAPI GCANNNNNTGC 2 cut(s) 830, 3344
BstC8I GCNNGC 4 cut(s) 930, 1956, 1960, 2608
BstDSI CCRYGG 3 cut(s) 399, 1455, 1712
BstFNI CGCG 1 cut(s) 3022
BstH2I RGCGCY 1 cut(s) 3256
BstHHI GCGC 2 cut(s) 3255, 3475
BstMAI GTCTC 5 cut(s) 1388, 1930, 2467, 2476, 2628
BstMWI GCNNNNNNNGC 7 cut(s) 485, 539, 830, 1556, 3019, 3148, 3344
BstNI CCWGG 9 cut(s) 132, 206, 519, 945, 1145, 1610, 1722, 2865, 3106
BstNSI RCATGY 1 cut(s) 1962
BstSFI CTRYAG 5 cut(s) 174, 336, 534, 2844, 2850
BstSLI GKGCMC 1 cut(s) 835
BstUI CGCG 1 cut(s) 3022
BstV1I GCAGC 8 cut(s) 160, 520, 586, 765, 1331, 1334, 1635, 2783
BstV2I GAAGAC 1 cut(s) 771
BstX2I RGATCY 6 cut(s) 125, 332, 1304, 2666, 2779, 3372
BstXI CCANNNNNNTGG 1 cut(s) 2690
BstYI RGATCY 6 cut(s) 125, 332, 1304, 2666, 2779, 3372
BsuI GTATCC 1 cut(s) 2603
BsuRI GGCC 5 cut(s) 978, 1287, 1720, 2313, 3097
BtgI CCRYGG 3 cut(s) 399, 1455, 1712
BtrI CACGTC 1 cut(s) 1940
BtsI GCAGTG 1 cut(s) 543
BveI ACCTGC 4 cut(s) 647, 1008, 1565, 2662
Cac8I GCNNGC 4 cut(s) 930, 1956, 1960, 2608
CaiI CAGNNNCTG 1 cut(s) 542
CciI TCATGA 4 cut(s) 79, 892, 3514, 3613
CfoI GCGC 2 cut(s) 3255, 3475
Cfr13I GGNCC 6 cut(s) 976, 997, 1039, 1807, 2312, 3260
Csp6I GTAC 3 cut(s) 1812, 2301, 2731
CspCI CAANNNNNGTGG 4 cut(s) 1297, 1332, 2878, 2913
CviQI GTAC 3 cut(s) 1812, 2301, 2731
DraI TTTAAA 1 cut(s) 1842
Eam1104I CTCTTC 5 cut(s) 1011, 1575, 1697, 2038, 3003
EarI CTCTTC 5 cut(s) 1011, 1575, 1697, 2038, 3003
EciI GGCGGA 2 cut(s) 915, 3526
Eco130I CCWWGG 2 cut(s) 1455, 1712
Eco31I GGTCTC 2 cut(s) 2467, 2628
Eco32I GATATC 1 cut(s) 1207
Eco47I GGWCC 4 cut(s) 997, 1039, 1807, 3260
Eco57I CTGAAG 4 cut(s) 888, 1035, 2747, 3287
Eco88I CYCGRG 1 cut(s) 574
EcoO109I RGGNCCY 2 cut(s) 976, 997
EcoRI GAATTC 5 cut(s) 253, 985, 2219, 2839, 3211
EcoRII CCWGG 9 cut(s) 130, 204, 517, 943, 1143, 1608, 1720, 2863, 3104
EcoRV GATATC 1 cut(s) 1207
EcoT14I CCWWGG 2 cut(s) 1455, 1712
EcoT22I ATGCAT 1 cut(s) 1355
ErhI CCWWGG 2 cut(s) 1455, 1712
Esp3I CGTCTC 2 cut(s) 1930, 2476
FalI AAGNNNNNCTT 2 cut(s) 1769, 1801
FaqI GGGAC 3 cut(s) 74, 1820, 2546
FauNDI CATATG 1 cut(s) 3579
FbaI TGATCA 2 cut(s) 3478, 3511
FspBI CTAG 5 cut(s) 48, 603, 1505, 1955, 3309
GlaI GCGC 2 cut(s) 3254, 3474
GsaI CCCAGC 2 cut(s) 621, 2661
GsuI CTGGAG 3 cut(s) 153, 966, 1166
HaeII RGCGCY 1 cut(s) 3256
HaeIII GGCC 5 cut(s) 978, 1287, 1720, 2313, 3097
HapII CCGG 1 cut(s) 2019
HhaI GCGC 2 cut(s) 3255, 3475
Hin6I GCGC 2 cut(s) 3253, 3473
HinP1I GCGC 2 cut(s) 3253, 3473
HincII GTYRAC 2 cut(s) 2137, 3289
HindII GTYRAC 2 cut(s) 2137, 3289
HindIII AAGCTT 2 cut(s) 2112, 2800
HpaI GTTAAC 1 cut(s) 2137
HpaII CCGG 1 cut(s) 2019
Hpy166II GTNNAC 9 cut(s) 581, 833, 1090, 1514, 2137, 2345, 2701, 2887, 3289
Hpy8I GTNNAC 9 cut(s) 581, 833, 1090, 1514, 2137, 2345, 2701, 2887, 3289
HpyCH4IV ACGT 2 cut(s) 1098, 1939
HpyF10VI GCNNNNNNNGC 7 cut(s) 485, 539, 830, 1556, 3019, 3148, 3344
HpySE526I ACGT 2 cut(s) 1098, 1939
HspAI GCGC 2 cut(s) 3253, 3473
Ksp22I TGATCA 2 cut(s) 3478, 3511
KspAI GTTAAC 1 cut(s) 2137
LguI GCTCTTC 1 cut(s) 3003
LmnI GCTCC 2 cut(s) 821, 2534
Lsp1109I GCAGC 8 cut(s) 160, 520, 586, 765, 1331, 1334, 1635, 2783
MaeI CTAG 5 cut(s) 48, 603, 1505, 1955, 3309
MaeII ACGT 2 cut(s) 1098, 1939
MaeIII GTNAC 9 cut(s) 812, 961, 1234, 1429, 1447, 2141, 2201, 2645, 2754
MfeI CAATTG 2 cut(s) 1912, 2093
MflI RGATCY 6 cut(s) 125, 332, 1304, 2666, 2779, 3372
MhlI GDGCHC 2 cut(s) 835, 1921
MlyI GAGTC 2 cut(s) 1157, 1714
MmeI TCCRAC 4 cut(s) 90, 603, 2415, 2664
Mph1103I ATGCAT 1 cut(s) 1355
MslI CAYNNNNRTG 4 cut(s) 810, 1101, 2508, 3513
MspI CCGG 1 cut(s) 2019
MunI CAATTG 2 cut(s) 1912, 2093
Mva1269I GAATGC 3 cut(s) 1895, 1909, 3178
MvaI CCWGG 9 cut(s) 132, 206, 519, 945, 1145, 1610, 1722, 2865, 3106
MvnI CGCG 1 cut(s) 3022
MwoI GCNNNNNNNGC 7 cut(s) 485, 539, 830, 1556, 3019, 3148, 3344
NciI CCSGG 1 cut(s) 2020
NcoI CCATGG 2 cut(s) 1455, 1712
NdeI CATATG 1 cut(s) 3579
NheI GCTAGC 1 cut(s) 1954
NlaIV GGNNCC 6 cut(s) 322, 977, 1306, 1808, 2000, 2126
NmuCI GTSAC 6 cut(s) 812, 1234, 1429, 2141, 2645, 2754
NsiI ATGCAT 1 cut(s) 1355
NspI RCATGY 1 cut(s) 1962
PaeI GCATGC 1 cut(s) 1962
PaeR7I CTCGAG 1 cut(s) 574
PagI TCATGA 4 cut(s) 79, 892, 3514, 3613
PaqCI CACCTGC 1 cut(s) 1565
PciSI GCTCTTC 1 cut(s) 3003
PctI GAATGC 3 cut(s) 1895, 1909, 3178
PflMI CCANNNNNTGG 1 cut(s) 2474
PfoI TCCNGGA 3 cut(s) 130, 517, 943
PleI GAGTC 2 cut(s) 1156, 1713
PpsI GAGTC 2 cut(s) 1156, 1713
PpuMI RGGWCCY 1 cut(s) 997
PshBI ATTAAT 1 cut(s) 2393
Psp5II RGGWCCY 1 cut(s) 997
Psp6I CCWGG 9 cut(s) 130, 204, 517, 943, 1143, 1608, 1720, 2863, 3104
PspFI CCCAGC 2 cut(s) 617, 2657
PspGI CCWGG 9 cut(s) 130, 204, 517, 943, 1143, 1608, 1720, 2863, 3104
PspN4I GGNNCC 6 cut(s) 322, 977, 1306, 1808, 2000, 2126
PspPI GGNCC 6 cut(s) 976, 997, 1039, 1807, 2312, 3260
PspPPI RGGWCCY 1 cut(s) 997
PspXI VCTCGAGB 1 cut(s) 574
PstI CTGCAG 2 cut(s) 178, 538
PstNI CAGNNNCTG 1 cut(s) 542
PsuI RGATCY 6 cut(s) 125, 332, 1304, 2666, 2779, 3372
RsaI GTAC 3 cut(s) 1813, 2302, 2732
RsaNI GTAC 3 cut(s) 1812, 2301, 2731
RseI CAYNNNNRTG 4 cut(s) 810, 1101, 2508, 3513
SapI GCTCTTC 1 cut(s) 3003
Sau96I GGNCC 6 cut(s) 976, 997, 1039, 1807, 2312, 3260
ScaI AGTACT 1 cut(s) 2732
SchI GAGTC 2 cut(s) 1157, 1714
SduI GDGCHC 2 cut(s) 835, 1921
SfcI CTRYAG 5 cut(s) 174, 336, 534, 2844, 2850
Sfr274I CTCGAG 1 cut(s) 574
SinI GGWCC 4 cut(s) 997, 1039, 1807, 3260
SlaI CTCGAG 1 cut(s) 574
SmiMI CAYNNNNRTG 4 cut(s) 810, 1101, 2508, 3513
SmlI CTYRAG 4 cut(s) 574, 795, 933, 3081
SmoI CTYRAG 4 cut(s) 574, 795, 933, 3081
SpeI ACTAGT 1 cut(s) 47
SphI GCATGC 1 cut(s) 1962
SspI AATATT 2 cut(s) 770, 1336
SspMI CTAG 5 cut(s) 48, 603, 1505, 1955, 3309
StyI CCWWGG 2 cut(s) 1455, 1712
TaiI ACGT 2 cut(s) 1101, 1942
TaqI TCGA 5 cut(s) 575, 1192, 1209, 1852, 1934
TaqII GACCGA 1 cut(s) 2160
TatI WGTACW 1 cut(s) 2730
TauI GCSGC 3 cut(s) 793, 2406, 3142
TseFI GTSAC 6 cut(s) 812, 1234, 1429, 2141, 2645, 2754
TseI GCWGC 8 cut(s) 173, 533, 599, 753, 1319, 1322, 1648, 2771
Tsp45I GTSAC 6 cut(s) 812, 1234, 1429, 2141, 2645, 2754
TspGWI ACGGA 4 cut(s) 388, 1533, 2500, 3426
Van91I CCANNNNNTGG 1 cut(s) 2474
VneI GTGCAC 1 cut(s) 831
VpaK11BI GGWCC 4 cut(s) 997, 1039, 1807, 3260
VspI ATTAAT 1 cut(s) 2393
XceI RCATGY 1 cut(s) 1962
XhoI CTCGAG 1 cut(s) 574
XspI CTAG 5 cut(s) 48, 603, 1505, 1955, 3309
ZrmI AGTACT 1 cut(s) 2732
Zsp2I ATGCAT 1 cut(s) 1355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.