Rroxscaffold_4G00292580

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003478
Physical Location & Seq
Forward (+)
12851152 .. 12856659
5508 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00292580.1

Sequence Viewer

Length: 3144 bp
ATGGACGAAAGCAAAGACAAGGGTGTTGCTGGTGAAATTATAGCTGGGGCTGTTGGGAGTCAGGGTCAGGTTAGTGAGGAAGGTGCGCCTTGTAATGGGGATGATCTAATGGTGGAGGTTTTCGGGTCTAATGTGTATGTGGGTGGAGTTTGTACTAGTGGGGGAGGGGATGAGGAGGATAAGGAGGTGGGTCGTGAGGAGTCAGTTGAGGTTGATATGGGTTCGGGAAGGAATGCAGGGTCTGTGGGTGGAGCTGGTGGTGAAGCGGGTTCGGGTGGGAAGGTAGAGTCGATGGAAGAAAGAAGGGGAGTGGTTGCGGTGGAGGAGGTAGGGGTGGTGGTTAGGGAGGAGAGTTTGATGGGTGCAGGGGCAGCTGCTGTTGCTGGTTCGGCTGGTGGAGAAACACAGGTAGTTGATGTTGAGAAAGCTGAAAAAGAAAGGAAACAAATTGGAGGACTTCTGGAGGAAGTTTTTGGGCGTGGGACTACGGATGGTGTGAAACAAGAAAAGAAGGTGGTTGGTGGTGGGGCTGATTTGAAGGTACAGAAAGTTAGTGTTGCAGATGACGAAGTAAGGAATCCTGGAATTGACACTGTAGCTGCAAGTTCTTCGGAAACAGTGGAAAATTCTTGTGTTCGGACCCAAGTTGTGGAAAAAGCTCCGGTTGTCAGTGAAGAAGGTTCGAGTCCCAAACTCTTGAATGGACAAAGGCTAGTATCTGCTGGTGGTGGTGCTGTTTCAGGCACGGACAAAGGAGATACTGTGACTTCTGAAGTTGCAGGTGTAGAGATTGGTAAAATTGATGAAAGTAATTCAGTAGGAAAACAGCAGGTTAAGATTGAGTCGCTAGGTGGAAACATCCATACTCACATCAATCTACCTGCACATATAGTTGCTTCGTCCCTGCCAGCTAAAGTAGTTCCTGTAGGTGAAATTGCTGTTACAGATGGAGAGGATCTCTTGAATTCTGAAAAAGATCATTTGAAATTAGAAGAGAGTGTAGAGAACATGTTTCATGATATTGCTCAGGTTGGATCAAATACTTCATCTCTGCCAGCAAAACTTCATCCTGGATGCATAGATCGAGAGGGTCTTTTGAATTTTGAAAGTCATCAGCACTTGAAACCCGAAGAGATTGTAGACAAGAACATGGTCCATGATATGACCCAGGTTGAATTAAATGGAGGACAAGAAATGGAAGTGGACAACCAAGTTAATGATGCTGAACATGGTGAGTTAGTTTCAGGAAATGATCAGAACTCAAAAATTGAGAAATTATGCATGAACTCTGTTGGGAAGGATCAAAAGTTGAAGTTTGAAGAGAGTTTAAACAATAGTGTGACCACTGACCATGCTCCGTCCAAGACCAATATTGTACATGAGGTTGAGGTAACTGAAGCTGATATGGATGGTTTATATGGAGAAAGCCTAGACATGGAAGTGGACAGAGATGCTAGTCATAGGGAACAACCAAAACCCAGTGAAGAGAAAATTGTTATTCAAGAAGCTATGCAGCCTGAAAGCTCTGAAATAGTGCCTCAGCTTAGATATGAGCTGCCACAAAAAAATGAAGGTACATTTTCTGTCTCAGATTTAGTTTGGGGTAAAGTGAAGAGCCATCCATGGTGGCCTGGACAGATATTTGATTTTATGGCTGCATCTGAGAAGGCAATGAAACATCATAAGAAGGACTGTTTTTTGGTAGCATATTTTGGGGACCGAACATTTGCTTGGAATGAAGTATCTAGTTTAAAGCCTTTCCAATCTTATTTCTCCCAGGCAGAGAAGCAGTGCAATTCAGAAACATTCCACAAAGCTGTCAATTGTGTTCTGGAAGAAGTTTCAAGACGTGTAGAATTGGGTCTATCATGCTCATGCATACCAAAAGATGTTTATGAAAAGATCAGATTCCAGATTATTGAAAATGCTGGGATATGTCATGAATCGAGTAGAGTGGACGGAGTAGATGAATCTGCAAGTGCAAGTTCATTTGAATCTGATAAACTACTTAAATATGTAAAAGCTTTAGCACAGTTCCCATCTGGCGGCAGTGGCAAATTAGAACTTGTCATTGCCAAGGCTCATTTGCTGGCATTTTTTCGTTTAAAGGGTTATTGTAGTTTGCCTGAATTCCAGTTTTGTGGAGATTTGTTGGAGAGTAAAACAGATAATTTGCTCTCTGGGGACAAAACATGTCCAGGTGAAATTACTGAGCACGCGACTTATATTGGGAAGGATGATAAGAAGACTGGACCGGAGATTGAGGAACTCAAGTCTAGCACTTCTCATAAACGCAAACATAATCTAAGAGAAGGAGCGTATGCTAAAATGAAAGAAAGAAGCCTGTCAGAATTAATGGGTGGCGAAACAGGTTCTCCAGATGGTAATGATTGGTTGGATGCGAAGGCTTCACCATCGTCTGGCAAGAGACGGAAGGGTGCTGAGTATCAGGCTGCTGATTTAGCCACACAAGATGGGAGGAAAGCTGTTTCCCATTCAACAGTTTCAAATACAACACCTTCACCAAAACCATCGTTTAAGATTGGTGAATGTATCCAGAGAGCTGCAAGCCAACTATCAGGGTCTACTATAGTGAAGAGCAGCACTGACAGACCTGCTGTGCAGGGATCTGATGTTCCCTTCCAGAGTTCCGATGATACTCTTAGGGGAGTGAATAATACAACAAAGTACTCATCTCTAGATGAATTGCTGTCACAACTTCGGTCAGCAGCAGAAGAACCTCTGAAGGAATACAATTCCTTGAGTACTATTGTCAATTTCTTCTCTGATTTTAGGAATTCTGTGGTTGTTGGCCAGAAATCTGGGGTGGCGCTTTCAGTTGTAGACAAAGTTGGTGGTAAAAAGAGGAAGTCAAATTCTGTGCATGGGTTACCTGAGACTTTTGAATTTGATGACATGAATGACACTTATTGGACAGACATGGTAATCCAAAATGGTGGAGAAGAGCAAGCATCACGGAAAAGAAAAACGAAGTATCAACCTGTCGTCCTTGCGCAACCAGAAAAGCCCCTCAAGTGGGTCGCAGGCCATACACCAGGAAGAAGTTTTCTCAAGGGAGTCAAGATTTGCCTCCAGAGAAACCTGTTGGCTATGTGGATGAGAATGCACCAGCAGAACTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

1047

Amino Acids

113.04

Weight (kDa)

5.11

Isoelectric Point (pI)

38.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 531 - 613 2.8e-17 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 770
Acc16I TGCGCA 1 cut(s) 3015
Acc36I ACCTGC 4 cut(s) 770, 820, 889, 2623
AccB7I CCANNNNNTGG 2 cut(s) 649, 2420
AccI GTMKAC 3 cut(s) 1140, 2585, 2844
AccII CGCG 1 cut(s) 2219
AciI CCGC 3 cut(s) 266, 317, 2046
AclWI GGATC 4 cut(s) 963, 1042, 1308, 2635
AcoI YGGCCR 1 cut(s) 2812
AcsI RAATTY 7 cut(s) 625, 964, 1099, 2129, 2797, 2875, 2906
AcuI CTGAAG 3 cut(s) 792, 1416, 2765
AfaI GTAC 6 cut(s) 154, 543, 1377, 1576, 2690, 2767
AfiI CCNNNNNNNGG 7 cut(s) 95, 649, 1435, 2045, 2420, 3036, 3037
AflIII ACRYGT 3 cut(s) 1008, 1849, 2192
AhlI ACTAGT 1 cut(s) 155
AjiI CACGTC 1 cut(s) 1850
AjnI CCWGG 7 cut(s) 580, 1069, 1167, 1630, 1776, 2197, 3055
AjuI GAANNNNNNNTTGG 2 cut(s) 1714, 1746
AloI GAACNNNNNNTCC 2 cut(s) 2619, 2651
Alw21I GWGCWC 1 cut(s) 2217
Alw26I GTCTC 3 cut(s) 1591, 2422, 2891
AlwI GGATC 4 cut(s) 963, 1042, 1308, 2635
AlwNI CAGNNNCTG 2 cut(s) 242, 377
AoxI GGCC 3 cut(s) 1628, 2812, 3046
ApoI RAATTY 7 cut(s) 625, 964, 1099, 2129, 2797, 2875, 2906
ArsI GACNNNNNNTTYG 2 cut(s) 1709, 1741
AseI ATTAAT 1 cut(s) 2354
AspLEI GCGC 3 cut(s) 88, 2833, 3016
AspS9I GGNCC 4 cut(s) 639, 1153, 1717, 2252
AsuHPI GGTGA 8 cut(s) 44, 272, 941, 1244, 2213, 2403, 2514, 2558
AvaII GGWCC 4 cut(s) 639, 1153, 1717, 2252
BalI TGGCCA 1 cut(s) 2814
BbsI GAAGAC 1 cut(s) 2252
Bbv12I GWGCWC 1 cut(s) 2217
BbvCI CCTCAGC 1 cut(s) 1539
BcgI CGANNNNNNTGC 2 cut(s) 591, 625
BciT130I CCWGG 7 cut(s) 582, 1071, 1169, 1632, 1778, 2199, 3057
BciVI GTATCC 1 cut(s) 2564
BclI TGATCA 1 cut(s) 1252
BcoDI GTCTC 3 cut(s) 1591, 2422, 2891
BcuI ACTAGT 1 cut(s) 155
BfaI CTAG 8 cut(s) 156, 713, 848, 1430, 1455, 1746, 2277, 2699
BfmI CTRYAG 3 cut(s) 594, 924, 2589
BfoI RGCGCY 1 cut(s) 2834
BfuAI ACCTGC 4 cut(s) 770, 820, 889, 2623
BfuI GTATCC 1 cut(s) 2564
BmcAI AGTACT 2 cut(s) 2690, 2767
Bme1390I CCNGG 7 cut(s) 582, 1071, 1169, 1632, 1778, 2199, 3057
Bme18I GGWCC 4 cut(s) 639, 1153, 1717, 2252
BmgBI CACGTC 1 cut(s) 1850
BmgT120I GGNCC 4 cut(s) 639, 1153, 1717, 2252
BmiI GGNNCC 2 cut(s) 641, 1718
BmrFI CCNGG 7 cut(s) 582, 1071, 1169, 1632, 1778, 2199, 3057
BmrI ACTGGG 1 cut(s) 1473
BmsI GCATC 6 cut(s) 1064, 1210, 1441, 1667, 2389, 2981
BmuI ACTGGG 1 cut(s) 1473
BpiI GAAGAC 1 cut(s) 2252
BplI GAGNNNNNCTC 2 cut(s) 942, 974
BpmI CTGGAG 3 cut(s) 482, 2361, 3077
Bpu10I CCTNAGC 2 cut(s) 1026, 1539
BpuEI CTTGAG 4 cut(s) 2255, 2782, 3017, 3056
BsaJI CCNNGG 4 cut(s) 1167, 1622, 1776, 2076
BsaWI WCCGGW 2 cut(s) 661, 2254
BsaXI ACNNNNNCTCC 6 cut(s) 49, 79, 747, 777, 2359, 2389
Bsc4I CCNNNNNNNGG 7 cut(s) 95, 649, 1435, 2045, 2420, 3036, 3037
Bse1I ACTGG 3 cut(s) 1479, 2134, 2254
Bse3DI GCAATG 2 cut(s) 1677, 2070
BseBI CCWGG 7 cut(s) 582, 1071, 1169, 1632, 1778, 2199, 3057
BseDI CCNNGG 4 cut(s) 1167, 1622, 1776, 2076
BseLI CCNNNNNNNGG 7 cut(s) 95, 649, 1435, 2045, 2420, 3036, 3037
BseMI GCAATG 2 cut(s) 1677, 2070
BseMII CTCAG 7 cut(s) 1040, 1553, 1602, 1653, 2202, 2433, 2886
BseNI ACTGG 3 cut(s) 1479, 2134, 2254
BseRI GAGGAG 4 cut(s) 188, 212, 338, 362
BseYI CCCAGC 2 cut(s) 44, 1928
BsgI GTGCAG 3 cut(s) 384, 867, 2642
Bsh1236I CGCG 1 cut(s) 2219
BshFI GGCC 3 cut(s) 1630, 2814, 3048
BsiHKAI GWGCWC 1 cut(s) 2217
BsiSI CCGG 2 cut(s) 662, 2255
BslFI GGGAC 5 cut(s) 496, 672, 886, 1730, 2198
BslI CCNNNNNNNGG 7 cut(s) 95, 649, 1435, 2045, 2420, 3036, 3037
BsmAI GTCTC 3 cut(s) 1591, 2422, 2891
BsmBI CGTCTC 1 cut(s) 2422
BsmFI GGGAC 5 cut(s) 496, 672, 886, 1730, 2198
BsmI GAATGC 2 cut(s) 238, 3129
BsnI GGCC 3 cut(s) 1630, 2814, 3048
Bsp1286I GDGCHC 1 cut(s) 2217
Bsp1407I TGTACA 1 cut(s) 1375
Bsp143I GATC 9 cut(s) 103, 955, 976, 1034, 1081, 1252, 1300, 1902, 2627
Bsp19I CCATGG 1 cut(s) 1622
BspACI CCGC 3 cut(s) 266, 317, 2046
BspANI GGCC 3 cut(s) 1630, 2814, 3048
BspCNI CTCAG 7 cut(s) 1039, 1552, 1601, 1654, 2203, 2434, 2887
BspFNI CGCG 1 cut(s) 2219
BspHI TCATGA 2 cut(s) 1015, 1939
BspLI GGNNCC 2 cut(s) 641, 1718
BspMI ACCTGC 4 cut(s) 770, 820, 889, 2623
BspPI GGATC 4 cut(s) 963, 1042, 1308, 2635
BspQI GCTCTTC 3 cut(s) 1607, 2591, 2958
BsrDI GCAATG 2 cut(s) 1677, 2070
BsrGI TGTACA 1 cut(s) 1375
BsrI ACTGG 3 cut(s) 1479, 2134, 2254
BssECI CCNNGG 4 cut(s) 1167, 1622, 1776, 2076
BssMI GATC 9 cut(s) 103, 955, 976, 1034, 1081, 1252, 1300, 1902, 2627
BssT1I CCWWGG 2 cut(s) 1622, 2076
Bst2UI CCWGG 7 cut(s) 582, 1071, 1169, 1632, 1778, 2199, 3057
Bst4CI ACNGT 6 cut(s) 595, 619, 763, 1694, 2034, 2503
Bst6I CTCTTC 7 cut(s) 987, 1125, 1314, 1479, 1607, 2591, 2958
BstAUI TGTACA 1 cut(s) 1375
BstC8I GCNNGC 7 cut(s) 909, 1056, 2091, 2217, 2569, 2970, 3046
BstDSI CCRYGG 1 cut(s) 1622
BstEII GGTNACC 1 cut(s) 2889
BstFNI CGCG 1 cut(s) 2219
BstH2I RGCGCY 1 cut(s) 2834
BstHHI GCGC 3 cut(s) 88, 2833, 3016
BstKTI GATC 9 cut(s) 106, 958, 979, 1037, 1084, 1255, 1303, 1905, 2630
BstMAI GTCTC 3 cut(s) 1591, 2422, 2891
BstMBI GATC 9 cut(s) 103, 955, 976, 1034, 1081, 1252, 1300, 1902, 2627
BstMWI GCNNNNNNNGC 5 cut(s) 371, 380, 389, 2052, 2462
BstNI CCWGG 7 cut(s) 582, 1071, 1169, 1632, 1778, 2199, 3057
BstNSI RCATGY 2 cut(s) 1012, 2196
BstPI GGTNACC 1 cut(s) 2889
BstSCI CCNGG 7 cut(s) 580, 1069, 1167, 1630, 1776, 2197, 3055
BstSFI CTRYAG 3 cut(s) 594, 924, 2589
BstUI CGCG 1 cut(s) 2219
BstV2I GAAGAC 1 cut(s) 2252
BstX2I RGATCY 2 cut(s) 955, 2627
BstXI CCANNNNNNTGG 3 cut(s) 2141, 2822, 2957
BstYI RGATCY 2 cut(s) 955, 2627
BsuI GTATCC 1 cut(s) 2564
BsuRI GGCC 3 cut(s) 1630, 2814, 3048
BtgI CCRYGG 1 cut(s) 1622
BtrI CACGTC 1 cut(s) 1850
BtsI GCAGTG 2 cut(s) 1796, 2056
BtsIMutI CAGTG 8 cut(s) 591, 624, 676, 1344, 1486, 1796, 2056, 2604
BveI ACCTGC 4 cut(s) 770, 820, 889, 2623
Cac8I GCNNGC 7 cut(s) 909, 1056, 2091, 2217, 2569, 2970, 3046
CaiI CAGNNNCTG 2 cut(s) 242, 377
CciI TCATGA 2 cut(s) 1015, 1939
CfoI GCGC 3 cut(s) 88, 2833, 3016
Cfr13I GGNCC 4 cut(s) 639, 1153, 1717, 2252
Csp6I GTAC 6 cut(s) 153, 542, 1376, 1575, 2689, 2766
CspCI CAANNNNNGTGG 2 cut(s) 2836, 2871
CviQI GTAC 6 cut(s) 153, 542, 1376, 1575, 2689, 2766
DpnI GATC 9 cut(s) 105, 957, 978, 1036, 1083, 1254, 1302, 1904, 2629
DpnII GATC 9 cut(s) 103, 955, 976, 1034, 1081, 1252, 1300, 1902, 2627
DraI TTTAAA 3 cut(s) 1329, 1752, 2106
EaeI YGGCCR 1 cut(s) 2812
Eam1104I CTCTTC 7 cut(s) 987, 1125, 1314, 1479, 1607, 2591, 2958
EarI CTCTTC 7 cut(s) 987, 1125, 1314, 1479, 1607, 2591, 2958
Eco130I CCWWGG 2 cut(s) 1622, 2076
Eco47I GGWCC 4 cut(s) 639, 1153, 1717, 2252
Eco57I CTGAAG 3 cut(s) 792, 1416, 2765
Eco91I GGTNACC 1 cut(s) 2889
EcoO65I GGTNACC 1 cut(s) 2889
EcoRI GAATTC 3 cut(s) 964, 2129, 2797
EcoRII CCWGG 7 cut(s) 580, 1069, 1167, 1630, 1776, 2197, 3055
EcoT14I CCWWGG 2 cut(s) 1622, 2076
EcoT22I ATGCAT 3 cut(s) 1079, 1283, 1880
ErhI CCWWGG 2 cut(s) 1622, 2076
Esp3I CGTCTC 1 cut(s) 2422
FaqI GGGAC 5 cut(s) 496, 672, 886, 1730, 2198
FauI CCCGC 1 cut(s) 259
FbaI TGATCA 1 cut(s) 1252
FblI GTMKAC 3 cut(s) 1140, 2585, 2844
FspBI CTAG 8 cut(s) 156, 713, 848, 1430, 1455, 1746, 2277, 2699
FspI TGCGCA 1 cut(s) 3015
GlaI GCGC 3 cut(s) 87, 2832, 3015
GsaI CCCAGC 2 cut(s) 48, 1932
GsuI CTGGAG 3 cut(s) 482, 2361, 3077
HaeII RGCGCY 1 cut(s) 2834
HaeIII GGCC 3 cut(s) 1630, 2814, 3048
HapII CCGG 2 cut(s) 662, 2255
HhaI GCGC 3 cut(s) 88, 2833, 3016
Hin6I GCGC 3 cut(s) 86, 2831, 3014
HinP1I GCGC 3 cut(s) 86, 2831, 3014
HindIII AAGCTT 1 cut(s) 2022
HpaII CCGG 2 cut(s) 662, 2255
HphI GGTGA 8 cut(s) 44, 272, 941, 1244, 2213, 2403, 2514, 2558
Hpy166II GTNNAC 6 cut(s) 1141, 1204, 1444, 1957, 2586, 2845
Hpy8I GTNNAC 6 cut(s) 1141, 1204, 1444, 1957, 2586, 2845
HpyCH4III ACNGT 6 cut(s) 595, 619, 763, 1694, 2034, 2503
HpyCH4IV ACGT 1 cut(s) 1849
HpyF10VI GCNNNNNNNGC 5 cut(s) 371, 380, 389, 2052, 2462
HpySE526I ACGT 1 cut(s) 1849
HspAI GCGC 3 cut(s) 86, 2831, 3014
Ksp22I TGATCA 1 cut(s) 1252
Kzo9I GATC 9 cut(s) 103, 955, 976, 1034, 1081, 1252, 1300, 1902, 2627
LguI GCTCTTC 3 cut(s) 1607, 2591, 2958
LmnI GCTCC 4 cut(s) 251, 664, 1360, 2315
LweI GCATC 6 cut(s) 1064, 1210, 1441, 1667, 2389, 2981
MaeI CTAG 8 cut(s) 156, 713, 848, 1430, 1455, 1746, 2277, 2699
MaeII ACGT 1 cut(s) 1849
MaeIII GTNAC 6 cut(s) 763, 940, 1339, 1390, 2712, 2889
MalI GATC 9 cut(s) 105, 957, 978, 1036, 1083, 1254, 1302, 1904, 2629
MboI GATC 9 cut(s) 103, 955, 976, 1034, 1081, 1252, 1300, 1902, 2627
MfeI CAATTG 1 cut(s) 1822
MflI RGATCY 2 cut(s) 955, 2627
MhlI GDGCHC 1 cut(s) 2217
MlsI TGGCCA 1 cut(s) 2814
MluNI TGGCCA 1 cut(s) 2814
MlyI GAGTC 6 cut(s) 67, 209, 296, 694, 851, 3087
MmeI TCCRAC 3 cut(s) 1012, 2133, 2376
Mox20I TGGCCA 1 cut(s) 2814
Mph1103I ATGCAT 3 cut(s) 1079, 1283, 1880
MscI TGGCCA 1 cut(s) 2814
MseI TTAA 9 cut(s) 834, 1178, 1215, 1328, 1751, 2010, 2105, 2354, 2538
MslI CAYNNNNRTG 2 cut(s) 1439, 1873
Msp20I TGGCCA 1 cut(s) 2814
MspA1I CMGCKG 1 cut(s) 374
MspI CCGG 2 cut(s) 662, 2255
MspR9I CCNGG 7 cut(s) 582, 1071, 1169, 1632, 1778, 2199, 3057
MssI GTTTAAAC 1 cut(s) 1329
MunI CAATTG 1 cut(s) 1822
Mva1269I GAATGC 2 cut(s) 238, 3129
MvaI CCWGG 7 cut(s) 582, 1071, 1169, 1632, 1778, 2199, 3057
MvnI CGCG 1 cut(s) 2219
MwoI GCNNNNNNNGC 5 cut(s) 371, 380, 389, 2052, 2462
NcoI CCATGG 1 cut(s) 1622
NdeII GATC 9 cut(s) 103, 955, 976, 1034, 1081, 1252, 1300, 1902, 2627
NlaIV GGNNCC 2 cut(s) 641, 1718
NmuCI GTSAC 3 cut(s) 763, 1339, 2712
NsbI TGCGCA 1 cut(s) 3015
NsiI ATGCAT 3 cut(s) 1079, 1283, 1880
NspI RCATGY 2 cut(s) 1012, 2196
PagI TCATGA 2 cut(s) 1015, 1939
PaqCI CACCTGC 1 cut(s) 770
PciI ACATGT 2 cut(s) 1008, 2192
PciSI GCTCTTC 3 cut(s) 1607, 2591, 2958
PctI GAATGC 2 cut(s) 238, 3129
PfeI GAWTC 5 cut(s) 577, 1908, 1943, 1970, 1994
PflMI CCANNNNNTGG 2 cut(s) 649, 2420
PfoI TCCNGGA 2 cut(s) 580, 1069
PleI GAGTC 6 cut(s) 66, 208, 295, 693, 850, 3086
PmeI GTTTAAAC 1 cut(s) 1329
PpsI GAGTC 6 cut(s) 66, 208, 295, 693, 850, 3086
PscI ACATGT 2 cut(s) 1008, 2192
PshBI ATTAAT 1 cut(s) 2354
Psp6I CCWGG 7 cut(s) 580, 1069, 1167, 1630, 1776, 2197, 3055
PspEI GGTNACC 1 cut(s) 2889
PspFI CCCAGC 2 cut(s) 44, 1928
PspGI CCWGG 7 cut(s) 580, 1069, 1167, 1630, 1776, 2197, 3055
PspN4I GGNNCC 2 cut(s) 641, 1718
PspPI GGNCC 4 cut(s) 639, 1153, 1717, 2252
PstNI CAGNNNCTG 2 cut(s) 242, 377
PsuI RGATCY 2 cut(s) 955, 2627
PvuII CAGCTG 1 cut(s) 374
RsaI GTAC 6 cut(s) 154, 543, 1377, 1576, 2690, 2767
RsaNI GTAC 6 cut(s) 153, 542, 1376, 1575, 2689, 2766
RseI CAYNNNNRTG 2 cut(s) 1439, 1873
SapI GCTCTTC 3 cut(s) 1607, 2591, 2958
SaqAI TTAA 9 cut(s) 834, 1178, 1215, 1328, 1751, 2010, 2105, 2354, 2538
Sau3AI GATC 9 cut(s) 103, 955, 976, 1034, 1081, 1252, 1300, 1902, 2627
Sau96I GGNCC 4 cut(s) 639, 1153, 1717, 2252
ScaI AGTACT 2 cut(s) 2690, 2767
SchI GAGTC 6 cut(s) 67, 209, 296, 694, 851, 3087
ScrFI CCNGG 7 cut(s) 582, 1071, 1169, 1632, 1778, 2199, 3057
SduI GDGCHC 1 cut(s) 2217
SfaNI GCATC 6 cut(s) 1064, 1210, 1441, 1667, 2389, 2981
SfcI CTRYAG 3 cut(s) 594, 924, 2589
SinI GGWCC 4 cut(s) 639, 1153, 1717, 2252
SmiMI CAYNNNNRTG 2 cut(s) 1439, 1873
SmlI CTYRAG 4 cut(s) 2270, 2761, 3032, 3071
SmoI CTYRAG 4 cut(s) 2270, 2761, 3032, 3071
SpeI ACTAGT 1 cut(s) 155
SsiI CCGC 3 cut(s) 266, 317, 2046
SspI AATATT 1 cut(s) 1372
SspMI CTAG 8 cut(s) 156, 713, 848, 1430, 1455, 1746, 2277, 2699
StyD4I CCNGG 7 cut(s) 580, 1069, 1167, 1630, 1776, 2197, 3055
StyI CCWWGG 2 cut(s) 1622, 2076
TaaI ACNGT 6 cut(s) 595, 619, 763, 1694, 2034, 2503
TaiI ACGT 1 cut(s) 1852
TaqI TCGA 4 cut(s) 290, 683, 1084, 1946
TaqII GACCGA 2 cut(s) 1734, 2712
TatI WGTACW 4 cut(s) 152, 1375, 2688, 2765
TauI GCSGC 1 cut(s) 2049
TfiI GAWTC 5 cut(s) 577, 1908, 1943, 1970, 1994
Tru1I TTAA 9 cut(s) 834, 1178, 1215, 1328, 1751, 2010, 2105, 2354, 2538
Tru9I TTAA 9 cut(s) 834, 1178, 1215, 1328, 1751, 2010, 2105, 2354, 2538
TscAI CASTG 8 cut(s) 598, 624, 676, 1351, 1486, 1796, 2056, 2611
TseFI GTSAC 3 cut(s) 763, 1339, 2712
Tsp45I GTSAC 3 cut(s) 763, 1339, 2712
TspGWI ACGGA 6 cut(s) 503, 761, 1347, 1974, 2446, 2992
TspRI CASTG 8 cut(s) 598, 624, 676, 1351, 1486, 1796, 2056, 2611
Van91I CCANNNNNTGG 2 cut(s) 649, 2420
VpaK11BI GGWCC 4 cut(s) 639, 1153, 1717, 2252
VspI ATTAAT 1 cut(s) 2354
XapI RAATTY 7 cut(s) 625, 964, 1099, 2129, 2797, 2875, 2906
XbaI TCTAGA 1 cut(s) 2698
XceI RCATGY 2 cut(s) 1012, 2196
XmiI GTMKAC 3 cut(s) 1140, 2585, 2844
XspI CTAG 8 cut(s) 156, 713, 848, 1430, 1455, 1746, 2277, 2699
ZrmI AGTACT 2 cut(s) 2690, 2767
Zsp2I ATGCAT 3 cut(s) 1079, 1283, 1880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.