Rorug01G0314300

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0003478
Physical Location & Seq
Reverse (-)
43205741 .. 43207505
1765 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0314300.1

Sequence Viewer

Length: 369 bp
ATGCTAACCATGGGAGGGTTAGCAAAGAATCGGACATGCATGTTTAGTTTTGGAGTCGTGGCCTTGGAAATTGCTTGCGGAAGGAGGACTTACCGGGATGGAAAATATCACTTGCTACTCTTCGGGTGGGTATGGCAATCATATCGCGAAGGTGTTCTTCTGGATGTAGCTGATGAGAAATTGGAAATGAATTATGATAGAAATGAAATGGAATGCTTGTTAATTGTGGGGTTATGGTGCAGTAACCCTAACGACAAAGAAAGACCAAAAGCGGGACAAGTAATGAAGATTCTTCAGTCTGAAGCACCAATGCCGGAACTTCCAGATGACATGCATAGTCAGCCACTGCCTCAAGATCTACAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

122

Amino Acids

14.02

Weight (kDa)

5.03

Isoelectric Point (pI)

64.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 147
AciI CCGC 2 cut(s) 78, 272
AcuI CTGAAG 2 cut(s) 278, 321
AfiI CCNNNNNNNGG 2 cut(s) 15, 272
AluBI AGCT 1 cut(s) 170
AluI AGCT 1 cut(s) 170
AlwNI CAGNNNCTG 1 cut(s) 346
AoxI GGCC 1 cut(s) 60
Asp700I GAANNNNTTC 1 cut(s) 153
AsuC2I CCSGG 1 cut(s) 95
BccI CCATC 1 cut(s) 92
BcgI CGANNNNNNTGC 2 cut(s) 125, 159
BcnI CCSGG 1 cut(s) 95
BfmI CTRYAG 1 cut(s) 359
BglII AGATCT 1 cut(s) 355
Bme1390I CCNGG 1 cut(s) 95
BmrFI CCNGG 1 cut(s) 95
BpuEI CTTGAG 1 cut(s) 336
BpuMI CCSGG 1 cut(s) 95
BsaJI CCNNGG 2 cut(s) 9, 63
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 272
BseDI CCNNGG 2 cut(s) 9, 63
BseGI GGATG 2 cut(s) 103, 169
BseLI CCNNNNNNNGG 2 cut(s) 15, 272
BsgI GTGCAG 1 cut(s) 259
Bsh1236I CGCG 1 cut(s) 147
BshFI GGCC 1 cut(s) 62
BsiSI CCGG 2 cut(s) 94, 314
BslFI GGGAC 1 cut(s) 288
BslI CCNNNNNNNGG 2 cut(s) 15, 272
BsmFI GGGAC 1 cut(s) 288
BsmI GAATGC 1 cut(s) 218
BsnI GGCC 1 cut(s) 62
Bsp143I GATC 1 cut(s) 355
Bsp19I CCATGG 1 cut(s) 9
Bsp68I TCGCGA 1 cut(s) 147
BspACI CCGC 2 cut(s) 78, 272
BspANI GGCC 1 cut(s) 62
BspFNI CGCG 1 cut(s) 147
BssECI CCNNGG 2 cut(s) 9, 63
BssMI GATC 1 cut(s) 355
BssT1I CCWWGG 2 cut(s) 9, 63
Bst6I CTCTTC 1 cut(s) 125
BstC8I GCNNGC 1 cut(s) 76
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 2 cut(s) 103, 169
BstFNI CGCG 1 cut(s) 147
BstKTI GATC 1 cut(s) 358
BstMBI GATC 1 cut(s) 355
BstMWI GCNNNNNNNGC 1 cut(s) 340
BstNSI RCATGY 3 cut(s) 39, 43, 334
BstSCI CCNGG 1 cut(s) 93
BstSFI CTRYAG 1 cut(s) 359
BstUI CGCG 1 cut(s) 147
BstX2I RGATCY 1 cut(s) 355
BstYI RGATCY 1 cut(s) 355
BsuRI GGCC 1 cut(s) 62
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 2 cut(s) 103, 169
BtsI GCAGTG 1 cut(s) 344
BtsIMutI CAGTG 1 cut(s) 344
BtuMI TCGCGA 1 cut(s) 147
Cac8I GCNNGC 1 cut(s) 76
CaiI CAGNNNCTG 1 cut(s) 346
CviAII CATG 4 cut(s) 10, 36, 40, 331
CviJI RGCY 3 cut(s) 62, 170, 343
CviKI_1 RGCY 3 cut(s) 62, 170, 343
DpnI GATC 1 cut(s) 357
DpnII GATC 1 cut(s) 355
Eam1104I CTCTTC 1 cut(s) 125
EarI CTCTTC 1 cut(s) 125
Eco130I CCWWGG 2 cut(s) 9, 63
Eco57I CTGAAG 2 cut(s) 278, 321
EcoT14I CCWWGG 2 cut(s) 9, 63
EcoT22I ATGCAT 2 cut(s) 41, 336
ErhI CCWWGG 2 cut(s) 9, 63
FaeI CATG 4 cut(s) 13, 39, 43, 334
FaiI YATR 9 cut(s) 11, 37, 41, 133, 142, 195, 235, 332, 336
FalI AAGNNNNNCTT 4 cut(s) 73, 105, 141, 173
FaqI GGGAC 1 cut(s) 288
FatI CATG 4 cut(s) 9, 35, 39, 330
FauI CCCGC 1 cut(s) 265
FokI GGATG 2 cut(s) 110, 176
HaeIII GGCC 1 cut(s) 62
HapII CCGG 2 cut(s) 94, 314
Hin1II CATG 4 cut(s) 13, 39, 43, 334
HinfI GANTC 3 cut(s) 28, 54, 289
HpaII CCGG 2 cut(s) 94, 314
Hpy188I TCNGA 2 cut(s) 33, 301
Hpy188III TCNNGA 4 cut(s) 146, 161, 323, 353
HpyAV CCTTC 2 cut(s) 75, 143
HpyCH4V TGCA 3 cut(s) 39, 240, 334
HpyF10VI GCNNNNNNNGC 1 cut(s) 340
Hsp92II CATG 4 cut(s) 13, 39, 43, 334
Kzo9I GATC 1 cut(s) 355
LpnPI CCDG 5 cut(s) 107, 146, 327, 336, 347
MaeIII GTNAC 1 cut(s) 242
MalI GATC 1 cut(s) 357
MboI GATC 1 cut(s) 355
MboII GAAGA 4 cut(s) 112, 149, 284, 298
MflI RGATCY 1 cut(s) 355
MluCI AATT 4 cut(s) 69, 179, 190, 222
MlyI GAGTC 1 cut(s) 63
MnlI CCTC 3 cut(s) 8, 78, 360
Mph1103I ATGCAT 2 cut(s) 41, 336
MroXI GAANNNNTTC 1 cut(s) 153
MseI TTAA 1 cut(s) 221
MspI CCGG 2 cut(s) 94, 314
MspR9I CCNGG 1 cut(s) 95
Mva1269I GAATGC 1 cut(s) 218
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 1 cut(s) 340
NciI CCSGG 1 cut(s) 95
NcoI CCATGG 1 cut(s) 9
NdeII GATC 1 cut(s) 355
NlaIII CATG 4 cut(s) 13, 39, 43, 334
NruI TCGCGA 1 cut(s) 147
NsiI ATGCAT 2 cut(s) 41, 336
NspI RCATGY 3 cut(s) 39, 43, 334
PctI GAATGC 1 cut(s) 218
PdmI GAANNNNTTC 1 cut(s) 153
PfeI GAWTC 2 cut(s) 28, 289
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
PstNI CAGNNNCTG 1 cut(s) 346
PsuI RGATCY 1 cut(s) 355
RruI TCGCGA 1 cut(s) 147
SaqAI TTAA 1 cut(s) 221
Sau3AI GATC 1 cut(s) 355
SchI GAGTC 1 cut(s) 63
ScrFI CCNGG 1 cut(s) 95
SetI ASST 2 cut(s) 154, 172
SfcI CTRYAG 1 cut(s) 359
SmlI CTYRAG 1 cut(s) 351
SmoI CTYRAG 1 cut(s) 351
Sse9I AATT 4 cut(s) 69, 179, 190, 222
SsiI CCGC 2 cut(s) 78, 272
StyD4I CCNGG 1 cut(s) 93
StyI CCWWGG 2 cut(s) 9, 63
TasI AATT 4 cut(s) 69, 179, 190, 222
TfiI GAWTC 2 cut(s) 28, 289
Tru1I TTAA 1 cut(s) 221
Tru9I TTAA 1 cut(s) 221
TscAI CASTG 1 cut(s) 351
TspDTI ATGAA 3 cut(s) 203, 219, 299
TspRI CASTG 1 cut(s) 351
XceI RCATGY 3 cut(s) 39, 43, 334
XmnI GAANNNNTTC 1 cut(s) 153
Zsp2I ATGCAT 2 cut(s) 41, 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.