FvH4_7g30340

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0007729
Physical Location & Seq
Reverse (-)
22120248 .. 22123920
3673 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g30340.t1

Sequence Viewer

Length: 1659 bp
ATGAGAGAGGATCATGTTCCGTGTTGCCAAGTCGATTTCTGGGTGTTCATAGTGATTAGTTTGATCCTTGTGTCACTTGCTGGTATTGCCTCTGGCCTTGCATTAGGACTCTTGTCCTTCAGCAAAGTTGATCTTGAGGTCCTCATTAGGTCCGGCCAACCCAAAGACCAGAAGAATGCAGCAACAATTCTGCCTCTTGTTAAGAATGAGCATTTACTGTTATGTACTCTCCTCATAGTCAAATCACTGGCAATGGAGGCACTTCCCATTTTCGTGGACTCAATTCTCCCAGTTTGGGCTGCCATTCTTGTCTCCGGCACACTTGTAGTTGCAGTTACAGAGATCATTCCTCAAGCTGTATGTTCTCGATATGGATTAAGTCTTGGTGCAAAAGGGTCTTACTTGGTACGATTACTTCTGGTGGTCTTTTTCCCCATAGCATACCCTTTCAGTAAGATGTTAGATTGCCTCCTTGGAAAAGAGCATTCTGCCCTCCTAAGACGAGCAGAGCTAAAAACATTGGTGGATCTGCATGCAAATGAGGCAGGGAAAGGTGGAGACTTGTCGCATCATGAAACTACGATTATTGGTGGTGCTTTGGATTTGACGCAAAAGACTGCTGAAGATGCAATGACTCCAATATCAGAAACCTTTTCCCTTGACATAAACTCCAAACTTGACATGAATACACTGGGTTCAATCATGAGCAGAGGTCACAGTCGTGTACCAATCTATTCAGGAAACCCAAACAATATCATTGGCCTCATTTTGGCAAAGAACTTAATCTTCTGTCGTCCTGAAGATGAAACCCCAGTTAAGTACATGACAATCAGAAGAATTCCAAGGGTTTATAGCAATTGGCCTTTATATGATATACTGAATCAGTTTCGAAAAGGCCACAGTCATATGGCTGCTGTGGTGAGGAATAAGAAGGATGTTAATGGACAAAGGGAAATAGATGCTAGGAGTTCAAGATTAGATTCAGTACGCGCAGAGAGCAAAGGTTTAAATTCTCCGATCAATGGGAAAGAGCATCCGAGTAACGCCATAGACATATCATCTGTGTATTCGAGTGAAAGTACTGAATACAGCACTCCACTCTCAGAATATGTCATGGAACAAGATGAAGATCTTACACAAACACATTACAAGCGCCATAAAAAGCAGGGCGGTGGAAATGTATCGAATGAAAGTGTAGGATCTATTCAAAGTCACTTGGATGAGGAGGTAATTGGGATCATAACAATGGAGGATGTTATGGAAGAACTGTTACAGGAAGACATACTGGATGAAACAGATGAATATGTGGATGTGCATAACAGAATTAAAATCAACCTGCTACCTTCAAGACGATCATCACCCTCAAAATCTCATAGAACCAAGTCTCTATCTCAAATTTATTGGAGAACTCCGGAGGCCTCTCCCTTGTCTTCGACATACAATAGTCCTATACTCCGTTCTCCCATTAATTCTGCATACATTCAACCACCTCCTATCTTCATCACAAGACCTACTTTGTATCCTTCCCCTCCACAAGCTGCACCATTTGCAGGCCCTATTCGCGCCTCGCCATCCTCTCATCGAGCCTCAAGAAAGTCATATGAGAAGCTAAGACAACACAGTGGACGTAAACCTGCAAGAACCTACTCGCTAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF21

Protein Analysis

553

Amino Acids

61.18

Weight (kDa)

8.09

Isoelectric Point (pI)

50.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNNM PF01595 19 - 188 2.2e-28 Cyclin M transmembrane N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1344, 1642
AccII CGCG 2 cut(s) 990, 1563
AccIII TCCGGA 1 cut(s) 1411
AciI CCGC 1 cut(s) 1170
AclWI GGATC 5 cut(s) 18, 58, 534, 1207, 1244
AcoI YGGCCR 1 cut(s) 154
AcsI RAATTY 3 cut(s) 837, 1009, 1395
AcuI CTGAAG 3 cut(s) 103, 642, 819
AfaI GTAC 6 cut(s) 226, 408, 726, 821, 987, 1081
AfiI CCNNNNNNNGG 4 cut(s) 295, 769, 1022, 1550
AgsI TTSAA 5 cut(s) 699, 972, 1208, 1347, 1484
AleI CACNNNNGTG 1 cut(s) 720
AluBI AGCT 4 cut(s) 356, 511, 1538, 1609
AluI AGCT 4 cut(s) 356, 511, 1538, 1609
Alw26I GTCTC 3 cut(s) 316, 552, 1389
AlwI GGATC 5 cut(s) 18, 58, 534, 1207, 1244
Aor13HI TCCGGA 1 cut(s) 1411
AoxI GGCC 7 cut(s) 94, 154, 760, 860, 895, 1416, 1552
ApeKI GCWGC 4 cut(s) 179, 299, 911, 1538
ApoI RAATTY 3 cut(s) 837, 1009, 1395
AseI ATTAAT 1 cut(s) 1467
AspLEI GCGC 3 cut(s) 992, 1155, 1565
AspS9I GGNCC 3 cut(s) 139, 150, 1553
AsuHPI GGTGA 2 cut(s) 931, 1350
AsuII TTCGAA 1 cut(s) 889
AvaII GGWCC 2 cut(s) 139, 150
BarI GAAGNNNNNNTAC 4 cut(s) 399, 431, 1254, 1286
BbsI GAAGAC 2 cut(s) 1284, 1422
BbvI GCAGC 4 cut(s) 191, 286, 898, 1525
BccI CCATC 1 cut(s) 1579
BciVI GTATCC 1 cut(s) 1530
BcoDI GTCTC 3 cut(s) 316, 552, 1389
BfaI CTAG 1 cut(s) 963
BfoI RGCGCY 1 cut(s) 1156
BfuAI ACCTGC 2 cut(s) 1344, 1642
BfuI GTATCC 1 cut(s) 1530
BglII AGATCT 1 cut(s) 1129
BisI GCNGC 4 cut(s) 180, 300, 912, 1539
BlsI GCNGC 4 cut(s) 181, 301, 913, 1540
BmcAI AGTACT 1 cut(s) 1081
Bme18I GGWCC 2 cut(s) 139, 150
BmgT120I GGNCC 3 cut(s) 139, 150, 1553
BmrI ACTGGG 3 cut(s) 284, 701, 806
BmsI GCATC 4 cut(s) 577, 616, 949, 1042
BmuI ACTGGG 3 cut(s) 284, 701, 806
BoxI GACNNNNGTC 1 cut(s) 112
BpiI GAAGAC 2 cut(s) 1284, 1422
Bpu14I TTCGAA 1 cut(s) 889
BpuEI CTTGAG 3 cut(s) 155, 336, 1573
BsaBI GATNNNNATC 1 cut(s) 1128
BsaJI CCNNGG 2 cut(s) 472, 842
BsaWI WCCGGW 1 cut(s) 1411
BsaXI ACNNNNNCTCC 2 cut(s) 653, 683
Bsc4I CCNNNNNNNGG 4 cut(s) 295, 769, 1022, 1550
Bse1I ACTGG 5 cut(s) 252, 290, 696, 812, 1290
Bse3DI GCAATG 2 cut(s) 258, 636
Bse8I GATNNNNATC 1 cut(s) 1128
BseAI TCCGGA 1 cut(s) 1411
BseDI CCNNGG 2 cut(s) 472, 842
BseGI GGATG 7 cut(s) 940, 1033, 1225, 1258, 1294, 1315, 1571
BseJI GATNNNNATC 1 cut(s) 1128
BseLI CCNNNNNNNGG 4 cut(s) 295, 769, 1022, 1550
BseMI GCAATG 2 cut(s) 258, 636
BseMII CTCAG 1 cut(s) 1116
BseNI ACTGG 5 cut(s) 252, 290, 696, 812, 1290
BseRI GAGGAG 2 cut(s) 221, 1238
BseXI GCAGC 4 cut(s) 191, 286, 898, 1525
BsgI GTGCAG 1 cut(s) 1524
Bsh1236I CGCG 2 cut(s) 990, 1563
BshFI GGCC 7 cut(s) 96, 156, 762, 862, 897, 1418, 1554
BsiSI CCGG 3 cut(s) 153, 315, 1412
BslI CCNNNNNNNGG 4 cut(s) 295, 769, 1022, 1550
BsmAI GTCTC 3 cut(s) 316, 552, 1389
BsmI GAATGC 2 cut(s) 181, 484
BsnI GGCC 7 cut(s) 96, 156, 762, 862, 897, 1418, 1554
Bsp119I TTCGAA 1 cut(s) 889
Bsp13I TCCGGA 1 cut(s) 1411
BspACI CCGC 1 cut(s) 1170
BspANI GGCC 7 cut(s) 96, 156, 762, 862, 897, 1418, 1554
BspCNI CTCAG 1 cut(s) 1115
BspEI TCCGGA 1 cut(s) 1411
BspFNI CGCG 2 cut(s) 990, 1563
BspHI TCATGA 2 cut(s) 571, 702
BspMI ACCTGC 2 cut(s) 1344, 1642
BspPI GGATC 5 cut(s) 18, 58, 534, 1207, 1244
BspT104I TTCGAA 1 cut(s) 889
BsrDI GCAATG 2 cut(s) 258, 636
BsrI ACTGG 5 cut(s) 252, 290, 696, 812, 1290
BssECI CCNNGG 2 cut(s) 472, 842
BssT1I CCWWGG 2 cut(s) 472, 842
Bst4CI ACNGT 5 cut(s) 219, 719, 902, 1269, 1622
BstAPI GCANNNNNTGC 1 cut(s) 1547
BstBI TTCGAA 1 cut(s) 889
BstC8I GCNNGC 2 cut(s) 534, 1552
BstDEI CTNAG 4 cut(s) 497, 1102, 1610, 1652
BstF5I GGATG 7 cut(s) 940, 1033, 1225, 1258, 1294, 1315, 1571
BstFNI CGCG 2 cut(s) 990, 1563
BstH2I RGCGCY 1 cut(s) 1156
BstHHI GCGC 3 cut(s) 992, 1155, 1565
BstMAI GTCTC 3 cut(s) 316, 552, 1389
BstMWI GCNNNNNNNGC 7 cut(s) 86, 257, 542, 626, 996, 1547, 1560
BstNSI RCATGY 1 cut(s) 536
BstPAI GACNNNNGTC 1 cut(s) 112
BstUI CGCG 2 cut(s) 990, 1563
BstV1I GCAGC 4 cut(s) 191, 286, 898, 1525
BstV2I GAAGAC 2 cut(s) 1284, 1422
BstX2I RGATCY 3 cut(s) 526, 1129, 1199
BstXI CCANNNNNNTGG 1 cut(s) 274
BstYI RGATCY 3 cut(s) 526, 1129, 1199
BsuI GTATCC 1 cut(s) 1530
BsuRI GGCC 7 cut(s) 96, 156, 762, 862, 897, 1418, 1554
BtsCI GGATG 7 cut(s) 940, 1033, 1225, 1258, 1294, 1315, 1571
BtsIMutI CAGTG 3 cut(s) 245, 689, 1627
BveI ACCTGC 2 cut(s) 1344, 1642
Cac8I GCNNGC 2 cut(s) 534, 1552
CciI TCATGA 2 cut(s) 571, 702
CfoI GCGC 3 cut(s) 992, 1155, 1565
Cfr13I GGNCC 3 cut(s) 139, 150, 1553
CseI GACGC 1 cut(s) 616
Csp6I GTAC 6 cut(s) 225, 407, 725, 820, 986, 1080
CviAII CATG 7 cut(s) 14, 533, 572, 682, 703, 823, 1114
CviQI GTAC 6 cut(s) 225, 407, 725, 820, 986, 1080
DdeI CTNAG 4 cut(s) 497, 1102, 1610, 1652
DraI TTTAAA 1 cut(s) 1008
EaeI YGGCCR 1 cut(s) 154
Eco130I CCWWGG 2 cut(s) 472, 842
Eco147I AGGCCT 1 cut(s) 1418
Eco47I GGWCC 2 cut(s) 139, 150
Eco57I CTGAAG 3 cut(s) 103, 642, 819
EcoO109I RGGNCCY 2 cut(s) 139, 1553
EcoRI GAATTC 1 cut(s) 837
EcoT14I CCWWGG 2 cut(s) 472, 842
ErhI CCWWGG 2 cut(s) 472, 842
FaeI CATG 7 cut(s) 17, 536, 575, 685, 706, 826, 1117
FalI AAGNNNNNCTT 4 cut(s) 117, 149, 1498, 1530
FatI CATG 7 cut(s) 13, 532, 571, 681, 702, 822, 1113
FauNDI CATATG 2 cut(s) 906, 1600
Fnu4HI GCNGC 4 cut(s) 180, 300, 912, 1539
FokI GGATG 7 cut(s) 947, 1020, 1232, 1265, 1301, 1322, 1558
Fsp4HI GCNGC 4 cut(s) 180, 300, 912, 1539
FspBI CTAG 1 cut(s) 963
GlaI GCGC 3 cut(s) 991, 1154, 1564
GluI GCNGC 4 cut(s) 180, 300, 912, 1539
HaeII RGCGCY 1 cut(s) 1156
HaeIII GGCC 7 cut(s) 96, 156, 762, 862, 897, 1418, 1554
HapII CCGG 3 cut(s) 153, 315, 1412
HgaI GACGC 1 cut(s) 616
HhaI GCGC 3 cut(s) 992, 1155, 1565
Hin1II CATG 7 cut(s) 17, 536, 575, 685, 706, 826, 1117
Hin6I GCGC 3 cut(s) 990, 1153, 1563
HinP1I GCGC 3 cut(s) 990, 1153, 1563
HinfI GANTC 5 cut(s) 108, 278, 634, 880, 980
HpaII CCGG 3 cut(s) 153, 315, 1412
HphI GGTGA 2 cut(s) 931, 1350
Hpy166II GTNNAC 4 cut(s) 277, 725, 1625, 1631
Hpy188I TCNGA 5 cut(s) 646, 833, 1017, 1038, 1105
Hpy8I GTNNAC 4 cut(s) 277, 725, 1625, 1631
HpyAV CCTTC 4 cut(s) 127, 925, 1353, 1533
HpyCH4III ACNGT 5 cut(s) 219, 719, 902, 1269, 1622
HpyCH4IV ACGT 1 cut(s) 1627
HpyF10VI GCNNNNNNNGC 7 cut(s) 86, 257, 542, 626, 996, 1547, 1560
HpyF3I CTNAG 4 cut(s) 497, 1102, 1610, 1652
HpySE526I ACGT 1 cut(s) 1627
Hsp92II CATG 7 cut(s) 17, 536, 575, 685, 706, 826, 1117
HspAI GCGC 3 cut(s) 990, 1153, 1563
Kpn2I TCCGGA 1 cut(s) 1411
Lsp1109I GCAGC 4 cut(s) 191, 286, 898, 1525
LweI GCATC 4 cut(s) 577, 616, 949, 1042
MaeI CTAG 1 cut(s) 963
MaeII ACGT 1 cut(s) 1627
MaeIII GTNAC 6 cut(s) 72, 334, 713, 1040, 1211, 1269
MfeI CAATTG 1 cut(s) 856
MflI RGATCY 3 cut(s) 526, 1129, 1199
MluCI AATT 9 cut(s) 186, 282, 837, 856, 1009, 1230, 1323, 1395, 1468
MlyI GAGTC 3 cut(s) 102, 272, 628
MroI TCCGGA 1 cut(s) 1411
MseI TTAA 8 cut(s) 201, 377, 782, 816, 939, 1007, 1326, 1467
MslI CAYNNNNRTG 5 cut(s) 272, 537, 720, 1218, 1244
MspI CCGG 3 cut(s) 153, 315, 1412
MunI CAATTG 1 cut(s) 856
Mva1269I GAATGC 2 cut(s) 181, 484
MvnI CGCG 2 cut(s) 990, 1563
MwoI GCNNNNNNNGC 7 cut(s) 86, 257, 542, 626, 996, 1547, 1560
NdeI CATATG 2 cut(s) 906, 1600
NlaIII CATG 7 cut(s) 17, 536, 575, 685, 706, 826, 1117
NmuCI GTSAC 3 cut(s) 72, 713, 1211
NspI RCATGY 1 cut(s) 536
NspV TTCGAA 1 cut(s) 889
OliI CACNNNNGTG 1 cut(s) 720
PaeI GCATGC 1 cut(s) 536
PagI TCATGA 2 cut(s) 571, 702
PceI AGGCCT 1 cut(s) 1418
PctI GAATGC 2 cut(s) 181, 484
PfeI GAWTC 2 cut(s) 880, 980
PkrI GCNGC 4 cut(s) 181, 301, 913, 1540
PleI GAGTC 3 cut(s) 102, 272, 628
PpsI GAGTC 3 cut(s) 102, 272, 628
PpuMI RGGWCCY 1 cut(s) 139
PshAI GACNNNNGTC 1 cut(s) 112
PshBI ATTAAT 1 cut(s) 1467
Psp5II RGGWCCY 1 cut(s) 139
PspPI GGNCC 3 cut(s) 139, 150, 1553
PspPPI RGGWCCY 1 cut(s) 139
PsuI RGATCY 3 cut(s) 526, 1129, 1199
RsaI GTAC 6 cut(s) 226, 408, 726, 821, 987, 1081
RsaNI GTAC 6 cut(s) 225, 407, 725, 820, 986, 1080
RseI CAYNNNNRTG 5 cut(s) 272, 537, 720, 1218, 1244
SaqAI TTAA 8 cut(s) 201, 377, 782, 816, 939, 1007, 1326, 1467
SatI GCNGC 4 cut(s) 180, 300, 912, 1539
Sau96I GGNCC 3 cut(s) 139, 150, 1553
ScaI AGTACT 1 cut(s) 1081
SchI GAGTC 3 cut(s) 102, 272, 628
SfaNI GCATC 4 cut(s) 577, 616, 949, 1042
SfuI TTCGAA 1 cut(s) 889
SinI GGWCC 2 cut(s) 139, 150
SmiMI CAYNNNNRTG 5 cut(s) 272, 537, 720, 1218, 1244
SmlI CTYRAG 3 cut(s) 134, 351, 1588
SmoI CTYRAG 3 cut(s) 134, 351, 1588
SphI GCATGC 1 cut(s) 536
Sse9I AATT 9 cut(s) 186, 282, 837, 856, 1009, 1230, 1323, 1395, 1468
SseBI AGGCCT 1 cut(s) 1418
SsiI CCGC 1 cut(s) 1170
SspMI CTAG 1 cut(s) 963
StuI AGGCCT 1 cut(s) 1418
StyI CCWWGG 2 cut(s) 472, 842
TaaI ACNGT 5 cut(s) 219, 719, 902, 1269, 1622
TaiI ACGT 1 cut(s) 1630
TaqI TCGA 7 cut(s) 33, 367, 889, 1070, 1184, 1433, 1582
TasI AATT 9 cut(s) 186, 282, 837, 856, 1009, 1230, 1323, 1395, 1468
TatI WGTACW 3 cut(s) 224, 819, 1079
TfiI GAWTC 2 cut(s) 880, 980
Tru1I TTAA 8 cut(s) 201, 377, 782, 816, 939, 1007, 1326, 1467
Tru9I TTAA 8 cut(s) 201, 377, 782, 816, 939, 1007, 1326, 1467
TscAI CASTG 3 cut(s) 252, 696, 1627
TseFI GTSAC 3 cut(s) 72, 713, 1211
TseI GCWGC 4 cut(s) 179, 299, 911, 1538
Tsp45I GTSAC 3 cut(s) 72, 713, 1211
TspDTI ATGAA 9 cut(s) 37, 588, 698, 819, 1140, 1203, 1305, 1314, 1489
TspGWI ACGGA 2 cut(s) 9, 1445
TspRI CASTG 3 cut(s) 252, 696, 1627
VpaK11BI GGWCC 2 cut(s) 139, 150
VspI ATTAAT 1 cut(s) 1467
XapI RAATTY 3 cut(s) 837, 1009, 1395
XceI RCATGY 1 cut(s) 536
XspI CTAG 1 cut(s) 963
ZrmI AGTACT 1 cut(s) 1081
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.