Rorug01G0409300

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0007729
Physical Location & Seq
Forward (+)
51508078 .. 51514352
6275 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0409300.1

Sequence Viewer

Length: 3441 bp
ATGATGATGATGGATAGGCTGAGTAAGAAAAAAGTTCTGCTTGTTCTTGATGATGTAGAAAACTTTAACCAAATTGAAGAGTTACTTGGAAGGCAGCATTCATTTGGTGGTGGGAGTAGAATCATAGTAACCACTAGGGATGTACAATCACTCAGTGGAGTTACTGCGAGATATAGTCCCAAGTTTCTAAGGTATGATGAAGCTCTTAAACTTTTTATGCAGTATGCCTTCAGAACAAACCAACCTACGAGAGAGTATGATCCTCTCTCAAGGCGTGCTGTAGAATATGCTCAAGGTCTACCTTTAGCACTCAAAGTTTTGGGAGCTAGTCTTCATGGCAAAAGTATAAGTGAGTGGGAAGATGAGCTAGAGAAAATAAAGGAAATCCCGCATAAGGGAATTGAGGGTGTGCTTAGAACAAGCTTTGATGGACTGGATCCTTCACAGCAGGACATCTTTCTAGACATTGCATGTTTCTTTAGAGGAATGAACGAAGGCTATGTAATCAAAATTCTGGAAAATTGTGGCTTCTATCCCCACAGTGGATTACAAGTACTACGTGATAGAGCTCTCTTAACTATCTCATACGGCAATAGGCTTGAAATGCATGATTTAATACAGGATATGGGTTGGCAAATCGTCCGCCAACAATCTATTAAAGAGCCTGGGAAGCGCAGTCGTTTGTGGACTTACGAAGACGTGGATCGTGTATTAGCTCAAAATACGGCTACAGAAGCAGTTGAAGGCATAATGCTAGACTTGTCGAAGTCAAAAGACATTGACATAGACGCTGAAGCTTTTGTTCGAATGACGAATCTAAGACTGCTCTTAATCGGTTATAACCACTACCTAGACTTCGATGCGAAAGATTTTCCATTGGAGCTGTTACACCAGCCAGGAGATGAGTGTAAACAACACGTCAGTGGGGTTGTAAAGTTTCTTTCTCGTGACTTGAGGTATCTCATGTGGCATGGATGCCCCCTAAAGTCCTTGCCATCCAATTTTTGCCCGAAGAATCTTGTGGATCTTGACATGCGTTTTAGCCACATTGAACAACTTTGGGAAGGAATCAAGCCTTTCAAAAAGTTGAAATTCATCAATTTGAGTCATTCTCATTACCTTACCACAACCCCCGACTTCACTGAAGCAACAAATGTGGAAACACTAGTTCTTGACGGTTGTTCAAGTTTACTTGATGTTAACTCATCCATTTCAGCTCTTAAAAACCTTGTGTTCTTGTGCCTAAGAGGATGCAAAGAACTTAAGAGTCTTCCAAGCAGCATATTTTTGAAGTCTCTTAAAATCCTTGATCTTTCTGGGTGCTCAAGTCTCGTAAAGTTTCCAGAGATTTCAGGGATTATGGACGACCTATCTGAAATTTTTCTAAATGAGACTGCAATAGAAGAGTTGCCCTCATCAATTGAACGGCTTCAGGGGCTTGTGTTATTACATTTGATAAACTGTAGAAGCCTTGTCCATCTTCCGGACAATATATGTAATTTGGTATGTCTTAAAGATCTCACTCTTTCTGGGTGCTCAAAGCTGTATCATTTGCCTGAGAACTTGGGGAATTTAGAATCCTTGATGTACCTTGAAGTAGAAGGAAGTGGTATAAGAGAACTCCCATTCTCTATCTTGTGCTTGAAAAGGCTGAAAACATTATCATGTGATGGATGTAAAGAAATGACGATGCCCTTTTCATCATGGTCATCATCAATTGAAGAATATAACTGTAGTTACTCTGGCCTGCAACATCTTGATTTAAGTGACAGCAATCTGTGGGAATTATCGGATGGTATTGCTCACTTGTCCTCATTGAAAACCTTAGAGCTGCGCAGAACCAACTTGGAGAGCTTACCTGCAACGATGAATCAACTTCGCCGTTTGACACGTCTTGAATTGGAAGCTTGCAAGAGACTGAAATCAATACCAGAGCTTCCATCAAGTATAAGTTACATAGATGCTCATGACTGCAGGGCTTTGGAAACCGTTGGAAAACCAAATACTCGGTGCTCTATGAATCTTTGCTTCACATTTTCTAATTGCCTCCAACTGGTACAAATGAATCTATTCAGAGATATTGTGGAAACTCATTCTCATCACCAGGGTAATTATCGACTTCCGCTTTACTTTAATATGTCTCTTCCCGGAAGTGAGATTCCTGACTGGTTCATTTATAAGTGTAGGGGATCCTCACTTACTGTACCGCTACCCCCAAATTGGTTTGATAATAAGTTTTTGGGGTTCGCTATATGTGCTGTTAGCGACTTCAAGGGTTCTCAGAATGATGCATCTGATCTATCCGCTCTATGTCGTTGTGGCTTAAAAGGAAATCATGGTGAGAGCAGTTTCTGTTTTACTTTGCTTGATTGGGGTTTCAGTACTGACAGAATCCTCGAGTCTGAGCATATGTTCATGGCATATGTGCCATGGTCTGAATATCGCTTCATTGAAGAGGGAAAGCTGGTCAATGAACGATATTTCACTGAGGCCACATTTGAGATTGTAGTAGAAAATAGAGTTGATAGACATGGTTTCGGGACAGTAATAAACCGCCATTGCATCACAAGTTGCGGAGTTCGTTTCTTTCATGGTGATTACCTGGGTTACAGGGAAGAGCAAATTCTAAGCATCAGCGAAACCAAATCTCATCATGACAGCTGTGACATCCTTTCGGTTAGTAGGAAAGGAAAAGAGGGGGTTGGGGTGCGGTTGGAGGAGTCTCCGGCGGACCGCAGCTCTGCCGGCGAGAGCAGGAAGAGGCCGGGTTATAAGGATCGGAGGCGTTCCTACAAGCTAAGAAAGAGTCTAGAGTCATGGACAATTGTTTCATCCACAGTTGATGATGGTCAGGCTTGGAGGAAATATGGCCAGAAGGAACTCCTCAATGCTCCATATCCAAGGGCTTACTTCAGATGTGCCCGCAAGTATGATCAAGGTTGCCAAGCAACCAAACAAGTCCAACAAGTCCAAGACACCCCAAAAGTGTACAAAACCACCTACATTGGAAACCACACGTGCAGAAACATGATCAGGGGTCCACAGATGATCATGGGCGCCACCGATCCTCCTACTTGGGATTCTCATGCTCCTCCTCGATCTACTGTTAGCTCGGAATCTGGATCGACCCCAATTTGCAATAAGAAAGAACATCACGGTGTTCATGGTGGTCATCTCCCTAGCTCGTCGTCTATGATTCCCTTGGTTCCGGTGAAGAAAGAGGAGTGCAAGGAGGGGACGACGTCGAGTGGTCTGACGGACAATCTTAACGATACAAGTGATGTGTGGCCGGAGTTCAAGGATGATCATCATCATCATTTTGGGTTCCCTGAGACTACTGTAATGTCTAATGAGAATGCGGTTTCGAACATGCAGTACTTGGACATGGACTTGGTGGTGAAGTCTATTAATTTCGAGAATGAGTTTGATTTTGATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF21

Protein Analysis

1146

Amino Acids

130.35

Weight (kDa)

6.05

Isoelectric Point (pI)

43.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 4 - 80 1.7e-07 NB-ARC domain
WHD_ROQ1 PF23282 149 - 218 1.9e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 339 - 358 6.2e-07 Leucine Rich Repeat
LRR_14 PF23598 402 - 510 3.3e-06 Leucine-rich repeat region
LRR_13 PF23286 429 - 515 9.6e-12 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 496 - 562 5.3e-10 Leucine-rich repeat region
LRR_13 PF23286 517 - 674 3.8e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 581 - 642 9.8e-10 Leucine-rich repeat region
LRR_8 PF13855 582 - 637 2.4e-07 Leucine rich repeat
C-JID PF20160 715 - 864 6.3e-20 C-JID domain
WRKY PF03106 948 - 1007 3e-21 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 840, 2178, 2772
AatII GACGTC 1 cut(s) 3245
Acc16I TGCGCA 1 cut(s) 1835
Acc36I ACCTGC 1 cut(s) 1867
AccB1I GGYRCC 1 cut(s) 3056
AccBSI CCGCTC 1 cut(s) 2306
AccI GTMKAC 1 cut(s) 298
AccIII TCCGGA 1 cut(s) 1483
AcoI YGGCCR 2 cut(s) 2869, 3287
AcsI RAATTY 5 cut(s) 510, 1091, 1377, 1570, 2622
AcuI CTGAAG 5 cut(s) 214, 813, 1164, 1415, 2896
AcvI CACGTG 1 cut(s) 3018
AcyI GRCGYC 2 cut(s) 3057, 3242
AdeI CACNNNGTG 1 cut(s) 155
AfaI GTAC 8 cut(s) 144, 555, 1589, 2058, 2205, 2383, 2990, 3377
AfiI CCNNNNNNNGG 6 cut(s) 394, 395, 542, 1483, 2053, 2220
AflII CTTAAG 1 cut(s) 1262
AflIII ACRYGT 3 cut(s) 916, 1889, 3015
AhlI ACTAGT 1 cut(s) 1165
AjiI CACGTC 3 cut(s) 700, 919, 1892
AjnI CCWGG 4 cut(s) 664, 895, 2103, 2601
AjuI GAANNNNNNNTTGG 2 cut(s) 69, 101
AleI CACNNNNGTG 1 cut(s) 921
Alw21I GWGCWC 4 cut(s) 571, 1325, 1538, 2015
Alw26I GTCTC 7 cut(s) 1299, 1334, 1385, 1909, 2145, 2727, 3326
AlwNI CAGNNNCTG 1 cut(s) 2352
Ama87I CYCGRG 1 cut(s) 2396
Aor13HI TCCGGA 1 cut(s) 1483
AoxI GGCC 5 cut(s) 1744, 2490, 2762, 2869, 3287
ApeKI GCWGC 4 cut(s) 94, 1278, 1831, 2736
ApoI RAATTY 5 cut(s) 510, 1091, 1377, 1570, 2622
AseI ATTAAT 1 cut(s) 3408
Asp700I GAANNNNTTC 3 cut(s) 1380, 1428, 2069
AspLEI GCGC 3 cut(s) 675, 1836, 3059
AspS9I GGNCC 2 cut(s) 2731, 3038
AsuC2I CCSGG 2 cut(s) 2148, 2766
AsuHPI GGTGA 5 cut(s) 2093, 2351, 2606, 3223, 3409
AsuII TTCGAA 2 cut(s) 805, 3365
AvaI CYCGRG 1 cut(s) 2396
AvaII GGWCC 2 cut(s) 2731, 3038
BaeGI GKGCMC 1 cut(s) 2923
BalI TGGCCA 1 cut(s) 2871
BamHI GGATCC 2 cut(s) 436, 2189
BanI GGYRCC 1 cut(s) 3056
BanII GRGCYC 1 cut(s) 571
BauI CACGAG 1 cut(s) 945
BbrPI CACGTG 1 cut(s) 3018
BbsI GAAGAC 3 cut(s) 323, 702, 1262
Bbv12I GWGCWC 4 cut(s) 571, 1325, 1538, 2015
BbvI GCAGC 4 cut(s) 106, 1290, 1818, 2748
BccI CCATC 8 cut(s) 4, 422, 1003, 1485, 1664, 1787, 1948, 2840
BceAI ACGGC 4 cut(s) 604, 741, 1442, 1866
BciT130I CCWGG 4 cut(s) 666, 897, 2105, 2603
BclI TGATCA 4 cut(s) 2932, 3030, 3048, 3304
BcnI CCSGG 2 cut(s) 2148, 2766
BcoDI GTCTC 7 cut(s) 1299, 1334, 1385, 1909, 2145, 2727, 3326
BcuI ACTAGT 1 cut(s) 1165
BfaI CTAG 9 cut(s) 135, 327, 368, 461, 755, 851, 1166, 2810, 3180
BfmI CTRYAG 5 cut(s) 279, 729, 1462, 1732, 1972
BfoI RGCGCY 1 cut(s) 3060
BfrI CTTAAG 1 cut(s) 1262
BfuAI ACCTGC 1 cut(s) 1867
BglII AGATCT 1 cut(s) 1516
BisI GCNGC 4 cut(s) 95, 1279, 1832, 2737
BlsI GCNGC 4 cut(s) 96, 1280, 1833, 2738
BmcAI AGTACT 3 cut(s) 555, 2383, 3377
Bme1390I CCNGG 6 cut(s) 666, 897, 2105, 2148, 2603, 2766
Bme18I GGWCC 2 cut(s) 2731, 3038
BmeT110I CYCGRG 1 cut(s) 2396
BmgBI CACGTC 3 cut(s) 700, 919, 1892
BmgT120I GGNCC 2 cut(s) 2731, 3038
BmiI GGNNCC 6 cut(s) 438, 2191, 3039, 3058, 3207, 3326
BmrFI CCNGG 6 cut(s) 666, 897, 2105, 2148, 2603, 2766
BmsI GCATC 9 cut(s) 850, 965, 1241, 1680, 1951, 2278, 2300, 2571, 2640
BpiI GAAGAC 3 cut(s) 323, 702, 1262
BplI GAGNNNNNCTC 4 cut(s) 1096, 1128, 1397, 1429
Bpu14I TTCGAA 2 cut(s) 805, 3365
BpuEI CTTGAG 4 cut(s) 253, 276, 973, 1309
BpuMI CCSGG 2 cut(s) 2148, 2766
BsaAI YACGTR 2 cut(s) 560, 3018
BsaBI GATNNNNATC 2 cut(s) 3306, 3309
BsaHI GRCGYC 2 cut(s) 3057, 3242
BsaJI CCNNGG 6 cut(s) 665, 2104, 2429, 2602, 2900, 3201
BsaWI WCCGGW 2 cut(s) 1483, 3208
BsaXI ACNNNNNCTCC 2 cut(s) 3052, 3082
Bsc4I CCNNNNNNNGG 6 cut(s) 394, 395, 542, 1483, 2053, 2220
Bse118I RCCGGY 1 cut(s) 2744
Bse1I ACTGG 3 cut(s) 438, 2058, 2171
Bse3DI GCAATG 2 cut(s) 465, 2557
Bse8I GATNNNNATC 2 cut(s) 3306, 3309
BseAI TCCGGA 1 cut(s) 1483
BseBI CCWGG 4 cut(s) 666, 897, 2105, 2603
BseDI CCNNGG 6 cut(s) 665, 2104, 2429, 2602, 2900, 3201
BseJI GATNNNNATC 2 cut(s) 3306, 3309
BseLI CCNNNNNNNGG 6 cut(s) 394, 395, 542, 1483, 2053, 2220
BseMI GCAATG 2 cut(s) 465, 2557
BseMII CTCAG 7 cut(s) 11, 166, 1548, 2294, 2394, 2478, 3321
BseNI ACTGG 3 cut(s) 438, 2058, 2171
BseRI GAGGAG 5 cut(s) 2732, 2873, 3081, 3084, 3236
BseSI GKGCMC 1 cut(s) 2923
BseXI GCAGC 4 cut(s) 106, 1290, 1818, 2748
BsgI GTGCAG 1 cut(s) 3040
BshFI GGCC 5 cut(s) 1746, 2492, 2764, 2871, 3289
BshNI GGYRCC 1 cut(s) 3056
BsiHKAI GWGCWC 4 cut(s) 571, 1325, 1538, 2015
BsiHKCI CYCGRG 1 cut(s) 2396
BsiSI CCGG 7 cut(s) 1484, 2148, 2726, 2745, 2765, 3209, 3290
BslFI GGGAC 3 cut(s) 162, 2554, 3250
BslI CCNNNNNNNGG 6 cut(s) 394, 395, 542, 1483, 2053, 2220
BsmAI GTCTC 7 cut(s) 1299, 1334, 1385, 1909, 2145, 2727, 3326
BsmFI GGGAC 3 cut(s) 162, 2554, 3250
BsmI GAATGC 2 cut(s) 97, 3361
BsnI GGCC 5 cut(s) 1746, 2492, 2764, 2871, 3289
BsoBI CYCGRG 1 cut(s) 2396
Bsp119I TTCGAA 2 cut(s) 805, 3365
Bsp1286I GDGCHC 5 cut(s) 571, 1325, 1538, 2015, 2923
Bsp13I TCCGGA 1 cut(s) 1483
Bsp1407I TGTACA 2 cut(s) 142, 2988
Bsp19I CCATGG 1 cut(s) 2429
BspANI GGCC 5 cut(s) 1746, 2492, 2764, 2871, 3289
BspCNI CTCAG 7 cut(s) 12, 165, 1549, 2293, 2395, 2479, 3322
BspEI TCCGGA 1 cut(s) 1483
BspHI TCATGA 2 cut(s) 1966, 2653
BspLI GGNNCC 6 cut(s) 438, 2191, 3039, 3058, 3207, 3326
BspMAI CTGCAG 1 cut(s) 1976
BspMI ACCTGC 1 cut(s) 1867
BspQI GCTCTTC 1 cut(s) 2610
BspT104I TTCGAA 2 cut(s) 805, 3365
BspT107I GGYRCC 1 cut(s) 3056
BspTI CTTAAG 1 cut(s) 1262
BsrBI CCGCTC 1 cut(s) 2306
BsrDI GCAATG 2 cut(s) 465, 2557
BsrFI RCCGGY 1 cut(s) 2744
BsrGI TGTACA 2 cut(s) 142, 2988
BsrI ACTGG 3 cut(s) 438, 2058, 2171
BssAI RCCGGY 1 cut(s) 2744
BssECI CCNNGG 6 cut(s) 665, 2104, 2429, 2602, 2900, 3201
BssNI GRCGYC 2 cut(s) 3057, 3242
BssSI CACGAG 1 cut(s) 945
BssT1I CCWWGG 3 cut(s) 2429, 2900, 3201
Bst2BI CACGAG 1 cut(s) 945
Bst2UI CCWGG 4 cut(s) 666, 897, 2105, 2603
Bst6I CTCTTC 6 cut(s) 72, 1398, 2148, 2448, 2610, 2753
BstACI GRCGYC 2 cut(s) 3057, 3242
BstAFI CTTAAG 1 cut(s) 1262
BstAUI TGTACA 2 cut(s) 142, 2988
BstBAI YACGTR 2 cut(s) 560, 3018
BstBI TTCGAA 2 cut(s) 805, 3365
BstC8I GCNNGC 5 cut(s) 276, 1748, 1909, 2746, 2923
BstDSI CCRYGG 1 cut(s) 2429
BstH2I RGCGCY 1 cut(s) 3060
BstHHI GCGC 3 cut(s) 675, 1836, 3059
BstMAI GTCTC 7 cut(s) 1299, 1334, 1385, 1909, 2145, 2727, 3326
BstMWI GCNNNNNNNGC 6 cut(s) 604, 670, 734, 1435, 2255, 2745
BstNI CCWGG 4 cut(s) 666, 897, 2105, 2603
BstNSI RCATGY 3 cut(s) 474, 1036, 3373
BstSCI CCNGG 6 cut(s) 664, 895, 2103, 2146, 2601, 2764
BstSFI CTRYAG 5 cut(s) 279, 729, 1462, 1732, 1972
BstSLI GKGCMC 1 cut(s) 2923
BstV1I GCAGC 4 cut(s) 106, 1290, 1818, 2748
BstV2I GAAGAC 3 cut(s) 323, 702, 1262
BstX2I RGATCY 4 cut(s) 436, 1024, 1516, 2189
BstYI RGATCY 4 cut(s) 436, 1024, 1516, 2189
BsuRI GGCC 5 cut(s) 1746, 2492, 2764, 2871, 3289
BtgI CCRYGG 1 cut(s) 2429
BtrI CACGTC 3 cut(s) 700, 919, 1892
BtsIMutI CAGTG 5 cut(s) 160, 547, 928, 1140, 2484
BveI ACCTGC 1 cut(s) 1867
Cac8I GCNNGC 5 cut(s) 276, 1748, 1909, 2746, 2923
CaiI CAGNNNCTG 1 cut(s) 2352
CciI TCATGA 2 cut(s) 1966, 2653
CfoI GCGC 3 cut(s) 675, 1836, 3059
Cfr10I RCCGGY 1 cut(s) 2744
Cfr13I GGNCC 2 cut(s) 2731, 3038
CpoI CGGWCCG 1 cut(s) 2731
CseI GACGC 1 cut(s) 797
Csp6I GTAC 8 cut(s) 143, 554, 1588, 2057, 2204, 2382, 2989, 3376
CspCI CAANNNNNGTGG 4 cut(s) 1137, 1172, 2986, 3021
CspI CGGWCCG 1 cut(s) 2731
CviQI GTAC 8 cut(s) 143, 554, 1588, 2057, 2204, 2382, 2989, 3376
DinI GGCGCC 1 cut(s) 3058
DraIII CACNNNGTG 1 cut(s) 155
EaeI YGGCCR 2 cut(s) 2869, 3287
Eam1104I CTCTTC 6 cut(s) 72, 1398, 2148, 2448, 2610, 2753
EarI CTCTTC 6 cut(s) 72, 1398, 2148, 2448, 2610, 2753
EciI GGCGGA 2 cut(s) 632, 2744
Ecl136II GAGCTC 1 cut(s) 569
Eco130I CCWWGG 3 cut(s) 2429, 2900, 3201
Eco24I GRGCYC 1 cut(s) 571
Eco47I GGWCC 2 cut(s) 2731, 3038
Eco53kI GAGCTC 1 cut(s) 569
Eco57I CTGAAG 5 cut(s) 214, 813, 1164, 1415, 2896
Eco72I CACGTG 1 cut(s) 3018
Eco88I CYCGRG 1 cut(s) 2396
EcoICRI GAGCTC 1 cut(s) 569
EcoRII CCWGG 4 cut(s) 664, 895, 2103, 2601
EcoT14I CCWWGG 3 cut(s) 2429, 2900, 3201
EcoT22I ATGCAT 2 cut(s) 609, 2293
EcoT38I GRGCYC 1 cut(s) 571
EgeI GGCGCC 1 cut(s) 3058
EheI GGCGCC 1 cut(s) 3058
ErhI CCWWGG 3 cut(s) 2429, 2900, 3201
FalI AAGNNNNNCTT 4 cut(s) 24, 56, 69, 101
FaqI GGGAC 3 cut(s) 162, 2554, 3250
FauI CCCGC 2 cut(s) 396, 2930
FauNDI CATATG 2 cut(s) 2409, 2422
FbaI TGATCA 4 cut(s) 2932, 3030, 3048, 3304
FblI GTMKAC 1 cut(s) 298
Fnu4HI GCNGC 4 cut(s) 95, 1279, 1832, 2737
FriOI GRGCYC 1 cut(s) 571
Fsp4HI GCNGC 4 cut(s) 95, 1279, 1832, 2737
FspBI CTAG 9 cut(s) 135, 327, 368, 461, 755, 851, 1166, 2810, 3180
FspI TGCGCA 1 cut(s) 1835
GlaI GCGC 3 cut(s) 674, 1835, 3058
GluI GCNGC 4 cut(s) 95, 1279, 1832, 2737
HaeII RGCGCY 1 cut(s) 3060
HaeIII GGCC 5 cut(s) 1746, 2492, 2764, 2871, 3289
HapII CCGG 7 cut(s) 1484, 2148, 2726, 2745, 2765, 3209, 3290
HgaI GACGC 1 cut(s) 797
HhaI GCGC 3 cut(s) 675, 1836, 3059
Hin1I GRCGYC 2 cut(s) 3057, 3242
Hin6I GCGC 3 cut(s) 673, 1834, 3057
HinP1I GCGC 3 cut(s) 673, 1834, 3057
HincII GTYRAC 1 cut(s) 1201
HindII GTYRAC 1 cut(s) 1201
HindIII AAGCTT 3 cut(s) 421, 795, 1905
HpaI GTTAAC 1 cut(s) 1201
HpaII CCGG 7 cut(s) 1484, 2148, 2726, 2745, 2765, 3209, 3290
HphI GGTGA 5 cut(s) 2093, 2351, 2606, 3223, 3409
Hpy166II GTNNAC 7 cut(s) 299, 687, 911, 1190, 1201, 2989, 3041
Hpy8I GTNNAC 7 cut(s) 299, 687, 911, 1190, 1201, 2989, 3041
Hpy99I CGWCG 3 cut(s) 3190, 3244, 3247
HpyAV CCTTC 8 cut(s) 84, 238, 450, 488, 737, 1058, 1595, 2869
HpyCH4IV ACGT 6 cut(s) 559, 699, 918, 1891, 3017, 3242
HpyF10VI GCNNNNNNNGC 6 cut(s) 604, 670, 734, 1435, 2255, 2745
HpySE526I ACGT 6 cut(s) 559, 699, 918, 1891, 3017, 3242
Hsp92I GRCGYC 2 cut(s) 3057, 3242
HspAI GCGC 3 cut(s) 673, 1834, 3057
KasI GGCGCC 1 cut(s) 3056
Kpn2I TCCGGA 1 cut(s) 1483
KroI GCCGGC 1 cut(s) 2744
KroNI GCCGGC 1 cut(s) 2746
Ksp22I TGATCA 4 cut(s) 2932, 3030, 3048, 3304
KspAI GTTAAC 1 cut(s) 1201
LguI GCTCTTC 1 cut(s) 2610
LmnI GCTCC 4 cut(s) 323, 880, 2896, 3094
Lsp1109I GCAGC 4 cut(s) 106, 1290, 1818, 2748
LweI GCATC 9 cut(s) 850, 965, 1241, 1680, 1951, 2278, 2300, 2571, 2640
MaeI CTAG 9 cut(s) 135, 327, 368, 461, 755, 851, 1166, 2810, 3180
MaeII ACGT 6 cut(s) 559, 699, 918, 1891, 3017, 3242
MbiI CCGCTC 1 cut(s) 2306
MfeI CAATTG 3 cut(s) 1419, 1716, 2823
MflI RGATCY 4 cut(s) 436, 1024, 1516, 2189
MhlI GDGCHC 5 cut(s) 571, 1325, 1538, 2015, 2923
MlsI TGGCCA 1 cut(s) 2871
MluNI TGGCCA 1 cut(s) 2871
Mly113I GGCGCC 1 cut(s) 3057
MlyI GAGTC 6 cut(s) 1114, 1276, 2408, 2729, 2815, 2822
MmeI TCCRAC 4 cut(s) 1972, 2074, 2694, 2986
Mox20I TGGCCA 1 cut(s) 2871
Mph1103I ATGCAT 2 cut(s) 609, 2293
MroI TCCGGA 1 cut(s) 1483
MroNI GCCGGC 1 cut(s) 2744
MroXI GAANNNNTTC 3 cut(s) 1380, 1428, 2069
MscI TGGCCA 1 cut(s) 2871
MslI CAYNNNNRTG 3 cut(s) 921, 1663, 3156
Msp20I TGGCCA 1 cut(s) 2871
MspA1I CMGCKG 1 cut(s) 2661
MspCI CTTAAG 1 cut(s) 1262
MspI CCGG 7 cut(s) 1484, 2148, 2726, 2745, 2765, 3209, 3290
MspR9I CCNGG 6 cut(s) 666, 897, 2105, 2148, 2603, 2766
MunI CAATTG 3 cut(s) 1419, 1716, 2823
Mva1269I GAATGC 2 cut(s) 97, 3361
MvaI CCWGG 4 cut(s) 666, 897, 2105, 2603
MwoI GCNNNNNNNGC 6 cut(s) 604, 670, 734, 1435, 2255, 2745
NaeI GCCGGC 1 cut(s) 2746
NarI GGCGCC 1 cut(s) 3057
NciI CCSGG 2 cut(s) 2148, 2766
NcoI CCATGG 1 cut(s) 2429
NdeI CATATG 2 cut(s) 2409, 2422
NgoMIV GCCGGC 1 cut(s) 2744
NlaIV GGNNCC 6 cut(s) 438, 2191, 3039, 3058, 3207, 3326
NmuCI GTSAC 3 cut(s) 947, 1766, 2663
NsbI TGCGCA 1 cut(s) 1835
NsiI ATGCAT 2 cut(s) 609, 2293
NspI RCATGY 3 cut(s) 474, 1036, 3373
NspV TTCGAA 2 cut(s) 805, 3365
OliI CACNNNNGTG 1 cut(s) 921
PaeR7I CTCGAG 1 cut(s) 2396
PagI TCATGA 2 cut(s) 1966, 2653
PciSI GCTCTTC 1 cut(s) 2610
PctI GAATGC 2 cut(s) 97, 3361
PdiI GCCGGC 1 cut(s) 2746
PdmI GAANNNNTTC 3 cut(s) 1380, 1428, 2069
PflFI GACNNNGTC 1 cut(s) 3241
PfoI TCCNGGA 1 cut(s) 2146
PkrI GCNGC 4 cut(s) 96, 1280, 1833, 2738
PleI GAGTC 6 cut(s) 1113, 1275, 2407, 2728, 2814, 2821
PluTI GGCGCC 1 cut(s) 3060
PmaCI CACGTG 1 cut(s) 3018
PmlI CACGTG 1 cut(s) 3018
PpsI GAGTC 6 cut(s) 1113, 1275, 2407, 2728, 2814, 2821
Ppu21I YACGTR 2 cut(s) 560, 3018
PshBI ATTAAT 1 cut(s) 3408
PsiI TTATAA 3 cut(s) 840, 2178, 2772
Psp124BI GAGCTC 1 cut(s) 571
Psp6I CCWGG 4 cut(s) 664, 895, 2103, 2601
PspCI CACGTG 1 cut(s) 3018
PspGI CCWGG 4 cut(s) 664, 895, 2103, 2601
PspN4I GGNNCC 6 cut(s) 438, 2191, 3039, 3058, 3207, 3326
PspPI GGNCC 2 cut(s) 2731, 3038
PspXI VCTCGAGB 1 cut(s) 2396
PsrI GAACNNNNNNTAC 2 cut(s) 3359, 3391
PstI CTGCAG 1 cut(s) 1976
PstNI CAGNNNCTG 1 cut(s) 2352
PsuI RGATCY 4 cut(s) 436, 1024, 1516, 2189
PsyI GACNNNGTC 1 cut(s) 3241
PvuII CAGCTG 1 cut(s) 2661
RsaI GTAC 8 cut(s) 144, 555, 1589, 2058, 2205, 2383, 2990, 3377
RsaNI GTAC 8 cut(s) 143, 554, 1588, 2057, 2204, 2382, 2989, 3376
RseI CAYNNNNRTG 3 cut(s) 921, 1663, 3156
Rsr2I CGGWCCG 1 cut(s) 2731
RsrII CGGWCCG 1 cut(s) 2731
SacI GAGCTC 1 cut(s) 571
SapI GCTCTTC 1 cut(s) 2610
SatI GCNGC 4 cut(s) 95, 1279, 1832, 2737
Sau96I GGNCC 2 cut(s) 2731, 3038
ScaI AGTACT 3 cut(s) 555, 2383, 3377
SchI GAGTC 6 cut(s) 1114, 1276, 2408, 2729, 2815, 2822
ScrFI CCNGG 6 cut(s) 666, 897, 2105, 2148, 2603, 2766
SduI GDGCHC 5 cut(s) 571, 1325, 1538, 2015, 2923
SfaNI GCATC 9 cut(s) 850, 965, 1241, 1680, 1951, 2278, 2300, 2571, 2640
SfcI CTRYAG 5 cut(s) 279, 729, 1462, 1732, 1972
SfoI GGCGCC 1 cut(s) 3058
Sfr274I CTCGAG 1 cut(s) 2396
SfuI TTCGAA 2 cut(s) 805, 3365
SinI GGWCC 2 cut(s) 2731, 3038
SlaI CTCGAG 1 cut(s) 2396
SmiMI CAYNNNNRTG 3 cut(s) 921, 1663, 3156
SmlI CTYRAG 6 cut(s) 268, 291, 952, 1262, 1324, 2396
SmoI CTYRAG 6 cut(s) 268, 291, 952, 1262, 1324, 2396
SpeI ACTAGT 1 cut(s) 1165
SspDI GGCGCC 1 cut(s) 3056
SspMI CTAG 9 cut(s) 135, 327, 368, 461, 755, 851, 1166, 2810, 3180
SstI GAGCTC 1 cut(s) 571
StyD4I CCNGG 6 cut(s) 664, 895, 2103, 2146, 2601, 2764
StyI CCWWGG 3 cut(s) 2429, 2900, 3201
TaiI ACGT 6 cut(s) 562, 702, 921, 1894, 3020, 3245
TatI WGTACW 5 cut(s) 142, 553, 2381, 2988, 3375
TscAI CASTG 5 cut(s) 160, 547, 928, 1147, 2491
TseFI GTSAC 3 cut(s) 947, 1766, 2663
TseI GCWGC 4 cut(s) 94, 1278, 1831, 2736
Tsp45I GTSAC 3 cut(s) 947, 1766, 2663
TspGWI ACGGA 1 cut(s) 3272
TspRI CASTG 5 cut(s) 160, 547, 928, 1147, 2491
Tth111I GACNNNGTC 1 cut(s) 3241
Vha464I CTTAAG 1 cut(s) 1262
VpaK11BI GGWCC 2 cut(s) 2731, 3038
VspI ATTAAT 1 cut(s) 3408
XapI RAATTY 5 cut(s) 510, 1091, 1377, 1570, 2622
XbaI TCTAGA 2 cut(s) 460, 2809
XceI RCATGY 3 cut(s) 474, 1036, 3373
XhoI CTCGAG 1 cut(s) 2396
XmiI GTMKAC 1 cut(s) 298
XmnI GAANNNNTTC 3 cut(s) 1380, 1428, 2069
XspI CTAG 9 cut(s) 135, 327, 368, 461, 755, 851, 1166, 2810, 3180
ZraI GACGTC 1 cut(s) 3243
ZrmI AGTACT 3 cut(s) 555, 2383, 3377
Zsp2I ATGCAT 2 cut(s) 609, 2293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.