FvH4_7g30360

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0007729
Physical Location & Seq
Reverse (-)
22130660 .. 22134031
3372 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g30360.t1

Sequence Viewer

Length: 1629 bp
ATGAGAGAGGATGATATGCCGTGTTGCCAGGTCGATTTCTGGGTGTTTATAGTGATCAGTTTGATCCTTGTGTCACTTGCTGGTATTGCATCTGGCCTTGCATTAGGACTCTTGTCTTTCAGCAAAATTGATCTTGAGGTCCTCATTAGGTCTGGCCAACCCAAAGACCAGAAGAATGCAGCAACAATTCTGCCTCTTGTTAAGAATGAGCATCTACTGTTATGTACCCTCCTCATAGTCAAATCACTGGCAATGGAGGCACTTCCCATTTTTGTGGACTCAGTTCTCCCCGTTTGGGCTGCCATTCTTGTGTCCGGCACGCTTGTAGTTGCATTTACAGAGATTATTCCTCAAGCTGTATGTTCTCGATATGGATTGCCTCTTGGTGCAAAAGGGTCTTACTTGGTACGATTACTTTTGGTGGTCTTTTTCCCCATAGCATACCCTTTCGGTAAGCTGTTGGATTGCCTCCTTGGAAAAGAGCCTTCTGCGCTCCTAAGACGAGCAGAGCTTAAAACATTGGTGGATCTTCATGCAAATGAGGCAGGGAAAGGTGGAGAGTTGTCGCATTATGAAACTACGATTATTGGTGGCGCTTTGGATTTGACGCAGAAGACTGCTGAAGATGCAATGACTCCAATATCAGAAACTTTTTCCCTTGACATAAACTCCAAACTTGACATGAATACACTGGGTTCAATCATGAGCAGAGGTCACAGTCGTGTACCAATCTATTCAGGAAACCCAAACAATATCATTGGCCTCATTTTGGCAAAGAATTTAATCTTCTGTCGCCCTGAAGATGAAACCCCAGTTAAGTACATGACAATCAGAAGAATTCCAAGGGTTTATAGCAACTGGCCTTTATATGATATACTGAATCAGTTCCGGAAAGGCCACAGTCATATGGCTGCTGTGGTGAGGAATAAGAAGGATGTTAATAGACAAAGGGAAATAGATGCTAGGAGTTCAAGATTAGATTCAATACGAGCAGAGAGCAAAGGTTTACATTCTCCGATCAATGAGAACGAGCATCTGAGTAAAACCATAGACATATCCTCTATGTATTCGAGTGAAATTACTGAATACAGCACTCCACTATCAGAACATGTCATGGAACAAGATGAAGATCTTACACAAACACATTACAAGCGCCATAAAAAGCAGGGCGGTGGAAATGTATCGAATGAAAGTGTAGGATCTATTCAAAGTCACTTAGATGAAGAGGTAATCGGAATCATAACAATGGAGGATGTTATGGAGGAACTGTTGCAGGAAGACATACTGGATGAAACAGATGAGTATGTGGATGTGCATAACAAAATTAAAATCAACCTGCTACCTTCAAGACGATCATCACCCTCAAAATCACATAGAACTAAGTCTCCATCTCAAATCTATTGGAGAAATCTGGAGGCCTCTCCTCTATCTTCGACATACAATAGTCCTATACTCCGTTCTCCCATAAATTCTGCATACATTCAACCACCTCCTATCTTCATCACAAGACCTACTTTGTATCCTTCCCCTGCTAAATCATCACTCAATACCAGTTCTCCACAAGCTGCACCGTTTGCAGGCTCTATTCGTGCCTCGCCGTCCTCACATCGAGCCTCAAGAGAGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF21

Protein Analysis

543

Amino Acids

60.08

Weight (kDa)

6.45

Isoelectric Point (pI)

56.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNNM PF01595 19 - 190 1.9e-28 Cyclin M transmembrane N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1344
AccIII TCCGGA 1 cut(s) 888
AciI CCGC 1 cut(s) 1170
AclWI GGATC 3 cut(s) 58, 534, 1207
AcoI YGGCCR 1 cut(s) 154
AcsI RAATTY 3 cut(s) 778, 837, 1468
AcuI CTGAAG 2 cut(s) 642, 819
AfaI GTAC 4 cut(s) 226, 408, 726, 821
AfiI CCNNNNNNNGG 3 cut(s) 295, 769, 1577
AflIII ACRYGT 1 cut(s) 1108
AgsI TTSAA 6 cut(s) 699, 972, 984, 1208, 1347, 1484
AjnI CCWGG 1 cut(s) 27
AleI CACNNNNGTG 1 cut(s) 720
AloI GAACNNNNNNTCC 2 cut(s) 1369, 1401
AluBI AGCT 4 cut(s) 356, 457, 511, 1565
AluI AGCT 4 cut(s) 356, 457, 511, 1565
Alw26I GTCTC 1 cut(s) 1389
AlwI GGATC 3 cut(s) 58, 534, 1207
Aor13HI TCCGGA 1 cut(s) 888
AoxI GGCC 6 cut(s) 94, 154, 760, 860, 895, 1416
ApeKI GCWGC 4 cut(s) 179, 299, 911, 1565
ApoI RAATTY 3 cut(s) 778, 837, 1468
Asp700I GAANNNNTTC 1 cut(s) 884
AspLEI GCGC 3 cut(s) 493, 596, 1155
AspS9I GGNCC 1 cut(s) 139
AsuHPI GGTGA 2 cut(s) 931, 1350
AvaII GGWCC 1 cut(s) 139
BalI TGGCCA 1 cut(s) 156
BbsI GAAGAC 2 cut(s) 620, 1284
BbvI GCAGC 4 cut(s) 191, 286, 898, 1552
BccI CCATC 1 cut(s) 1396
BceAI ACGGC 2 cut(s) 4, 1582
BciT130I CCWGG 1 cut(s) 29
BciVI GTATCC 1 cut(s) 1530
BclI TGATCA 1 cut(s) 54
BcoDI GTCTC 1 cut(s) 1389
BfaI CTAG 1 cut(s) 963
BfoI RGCGCY 2 cut(s) 597, 1156
BfuAI ACCTGC 1 cut(s) 1344
BfuI GTATCC 1 cut(s) 1530
BglII AGATCT 1 cut(s) 1129
BisI GCNGC 4 cut(s) 180, 300, 912, 1566
BlsI GCNGC 4 cut(s) 181, 301, 913, 1567
Bme1390I CCNGG 1 cut(s) 29
Bme18I GGWCC 1 cut(s) 139
BmgT120I GGNCC 1 cut(s) 139
BmrFI CCNGG 1 cut(s) 29
BmrI ACTGGG 2 cut(s) 701, 806
BmsI GCATC 5 cut(s) 98, 220, 616, 949, 1042
BmuI ACTGGG 2 cut(s) 701, 806
BoxI GACNNNNGTC 1 cut(s) 112
BpiI GAAGAC 2 cut(s) 620, 1284
BpmI CTGGAG 1 cut(s) 1433
BpuEI CTTGAG 3 cut(s) 155, 336, 1600
BsaBI GATNNNNATC 1 cut(s) 1128
BsaJI CCNNGG 2 cut(s) 472, 842
BsaWI WCCGGW 1 cut(s) 888
BsaXI ACNNNNNCTCC 8 cut(s) 653, 683, 1253, 1283, 1369, 1399, 1540, 1570
Bsc4I CCNNNNNNNGG 3 cut(s) 295, 769, 1577
Bse1I ACTGG 6 cut(s) 252, 696, 812, 863, 1290, 1551
Bse3DI GCAATG 2 cut(s) 258, 636
Bse8I GATNNNNATC 1 cut(s) 1128
BseAI TCCGGA 1 cut(s) 888
BseBI CCWGG 1 cut(s) 29
BseDI CCNNGG 2 cut(s) 472, 842
BseGI GGATG 5 cut(s) 16, 940, 1258, 1294, 1315
BseJI GATNNNNATC 1 cut(s) 1128
BseLI CCNNNNNNNGG 3 cut(s) 295, 769, 1577
BseMI GCAATG 2 cut(s) 258, 636
BseMII CTCAG 2 cut(s) 294, 1028
BseNI ACTGG 6 cut(s) 252, 696, 812, 863, 1290, 1551
BseRI GAGGAG 2 cut(s) 221, 1413
BseXI GCAGC 4 cut(s) 191, 286, 898, 1552
BsgI GTGCAG 1 cut(s) 1551
BshFI GGCC 6 cut(s) 96, 156, 762, 862, 897, 1418
BsiSI CCGG 2 cut(s) 315, 889
BslI CCNNNNNNNGG 3 cut(s) 295, 769, 1577
BsmAI GTCTC 1 cut(s) 1389
BsmI GAATGC 1 cut(s) 181
BsnI GGCC 6 cut(s) 96, 156, 762, 862, 897, 1418
Bsp13I TCCGGA 1 cut(s) 888
Bsp143I GATC 8 cut(s) 54, 63, 130, 526, 1017, 1129, 1199, 1352
BspACI CCGC 1 cut(s) 1170
BspANI GGCC 6 cut(s) 96, 156, 762, 862, 897, 1418
BspCNI CTCAG 2 cut(s) 293, 1029
BspEI TCCGGA 1 cut(s) 888
BspHI TCATGA 1 cut(s) 702
BspMI ACCTGC 1 cut(s) 1344
BspPI GGATC 3 cut(s) 58, 534, 1207
BsrDI GCAATG 2 cut(s) 258, 636
BsrI ACTGG 6 cut(s) 252, 696, 812, 863, 1290, 1551
BssECI CCNNGG 2 cut(s) 472, 842
BssMI GATC 8 cut(s) 54, 63, 130, 526, 1017, 1129, 1199, 1352
BssT1I CCWWGG 2 cut(s) 472, 842
Bst2UI CCWGG 1 cut(s) 29
Bst4CI ACNGT 5 cut(s) 219, 719, 902, 1269, 1572
Bst6I CTCTTC 1 cut(s) 1218
BstAPI GCANNNNNTGC 1 cut(s) 1574
BstC8I GCNNGC 2 cut(s) 320, 1579
BstDEI CTNAG 5 cut(s) 280, 497, 1037, 1216, 1380
BstF5I GGATG 5 cut(s) 16, 940, 1258, 1294, 1315
BstH2I RGCGCY 2 cut(s) 597, 1156
BstHHI GCGC 3 cut(s) 493, 596, 1155
BstKTI GATC 8 cut(s) 57, 66, 133, 529, 1020, 1132, 1202, 1355
BstMAI GTCTC 1 cut(s) 1389
BstMBI GATC 8 cut(s) 54, 63, 130, 526, 1017, 1129, 1199, 1352
BstMWI GCNNNNNNNGC 6 cut(s) 86, 257, 490, 542, 626, 1574
BstNI CCWGG 1 cut(s) 29
BstNSI RCATGY 1 cut(s) 1112
BstPAI GACNNNNGTC 1 cut(s) 112
BstSCI CCNGG 1 cut(s) 27
BstV1I GCAGC 4 cut(s) 191, 286, 898, 1552
BstV2I GAAGAC 2 cut(s) 620, 1284
BstX2I RGATCY 3 cut(s) 526, 1129, 1199
BstXI CCANNNNNNTGG 1 cut(s) 274
BstYI RGATCY 3 cut(s) 526, 1129, 1199
BsuI GTATCC 1 cut(s) 1530
BsuRI GGCC 6 cut(s) 96, 156, 762, 862, 897, 1418
BtsCI GGATG 5 cut(s) 16, 940, 1258, 1294, 1315
BtsIMutI CAGTG 2 cut(s) 245, 689
BveI ACCTGC 1 cut(s) 1344
Cac8I GCNNGC 2 cut(s) 320, 1579
CciI TCATGA 1 cut(s) 702
CfoI GCGC 3 cut(s) 493, 596, 1155
Cfr13I GGNCC 1 cut(s) 139
CseI GACGC 1 cut(s) 616
Csp6I GTAC 4 cut(s) 225, 407, 725, 820
CviAII CATG 6 cut(s) 533, 682, 703, 823, 1109, 1114
CviQI GTAC 4 cut(s) 225, 407, 725, 820
DdeI CTNAG 5 cut(s) 280, 497, 1037, 1216, 1380
DpnI GATC 8 cut(s) 56, 65, 132, 528, 1019, 1131, 1201, 1354
DpnII GATC 8 cut(s) 54, 63, 130, 526, 1017, 1129, 1199, 1352
EaeI YGGCCR 1 cut(s) 154
Eam1104I CTCTTC 1 cut(s) 1218
EarI CTCTTC 1 cut(s) 1218
Eco130I CCWWGG 2 cut(s) 472, 842
Eco147I AGGCCT 1 cut(s) 1418
Eco47I GGWCC 1 cut(s) 139
Eco57I CTGAAG 2 cut(s) 642, 819
EcoO109I RGGNCCY 1 cut(s) 139
EcoRI GAATTC 1 cut(s) 837
EcoRII CCWGG 1 cut(s) 27
EcoT14I CCWWGG 2 cut(s) 472, 842
ErhI CCWWGG 2 cut(s) 472, 842
FaeI CATG 6 cut(s) 536, 685, 706, 826, 1112, 1117
FalI AAGNNNNNCTT 2 cut(s) 1498, 1530
FatI CATG 6 cut(s) 532, 681, 702, 822, 1108, 1113
FauNDI CATATG 1 cut(s) 906
FbaI TGATCA 1 cut(s) 54
Fnu4HI GCNGC 4 cut(s) 180, 300, 912, 1566
FokI GGATG 5 cut(s) 23, 947, 1265, 1301, 1322
Fsp4HI GCNGC 4 cut(s) 180, 300, 912, 1566
FspBI CTAG 1 cut(s) 963
GlaI GCGC 3 cut(s) 492, 595, 1154
GluI GCNGC 4 cut(s) 180, 300, 912, 1566
GsuI CTGGAG 1 cut(s) 1433
HaeII RGCGCY 2 cut(s) 597, 1156
HaeIII GGCC 6 cut(s) 96, 156, 762, 862, 897, 1418
HapII CCGG 2 cut(s) 315, 889
HgaI GACGC 1 cut(s) 616
HhaI GCGC 3 cut(s) 493, 596, 1155
Hin1II CATG 6 cut(s) 536, 685, 706, 826, 1112, 1117
Hin6I GCGC 3 cut(s) 491, 594, 1153
HinP1I GCGC 3 cut(s) 491, 594, 1153
HinfI GANTC 7 cut(s) 108, 278, 634, 880, 980, 1236, 1622
HpaII CCGG 2 cut(s) 315, 889
HphI GGTGA 2 cut(s) 931, 1350
Hpy166II GTNNAC 3 cut(s) 277, 725, 1007
Hpy188I TCNGA 6 cut(s) 646, 833, 1017, 1038, 1105, 1235
Hpy188III TCNNGA 9 cut(s) 134, 366, 703, 738, 889, 972, 1347, 1412, 1617
Hpy8I GTNNAC 3 cut(s) 277, 725, 1007
HpyAV CCTTC 4 cut(s) 495, 925, 1353, 1533
HpyCH4III ACNGT 5 cut(s) 219, 719, 902, 1269, 1572
HpyF10VI GCNNNNNNNGC 6 cut(s) 86, 257, 490, 542, 626, 1574
HpyF3I CTNAG 5 cut(s) 280, 497, 1037, 1216, 1380
Hsp92II CATG 6 cut(s) 536, 685, 706, 826, 1112, 1117
HspAI GCGC 3 cut(s) 491, 594, 1153
Kpn2I TCCGGA 1 cut(s) 888
Ksp22I TGATCA 1 cut(s) 54
Kzo9I GATC 8 cut(s) 54, 63, 130, 526, 1017, 1129, 1199, 1352
LmnI GCTCC 1 cut(s) 498
Lsp1109I GCAGC 4 cut(s) 191, 286, 898, 1552
LweI GCATC 5 cut(s) 98, 220, 616, 949, 1042
MaeI CTAG 1 cut(s) 963
MaeIII GTNAC 3 cut(s) 72, 713, 1211
MalI GATC 8 cut(s) 56, 65, 132, 528, 1019, 1131, 1201, 1354
MboI GATC 8 cut(s) 54, 63, 130, 526, 1017, 1129, 1199, 1352
MflI RGATCY 3 cut(s) 526, 1129, 1199
MlsI TGGCCA 1 cut(s) 156
MluCI AATT 7 cut(s) 126, 186, 778, 837, 1077, 1323, 1468
MluNI TGGCCA 1 cut(s) 156
MlyI GAGTC 3 cut(s) 102, 272, 628
MmeI TCCRAC 1 cut(s) 441
Mox20I TGGCCA 1 cut(s) 156
MroI TCCGGA 1 cut(s) 888
MroXI GAANNNNTTC 1 cut(s) 884
MscI TGGCCA 1 cut(s) 156
MseI TTAA 6 cut(s) 201, 513, 782, 816, 939, 1326
MslI CAYNNNNRTG 6 cut(s) 272, 308, 537, 720, 1218, 1244
Msp20I TGGCCA 1 cut(s) 156
MspI CCGG 2 cut(s) 315, 889
MspR9I CCNGG 1 cut(s) 29
Mva1269I GAATGC 1 cut(s) 181
MvaI CCWGG 1 cut(s) 29
MwoI GCNNNNNNNGC 6 cut(s) 86, 257, 490, 542, 626, 1574
NdeI CATATG 1 cut(s) 906
NdeII GATC 8 cut(s) 54, 63, 130, 526, 1017, 1129, 1199, 1352
NlaIII CATG 6 cut(s) 536, 685, 706, 826, 1112, 1117
NmuCI GTSAC 3 cut(s) 72, 713, 1211
NspI RCATGY 1 cut(s) 1112
OliI CACNNNNGTG 1 cut(s) 720
PagI TCATGA 1 cut(s) 702
PceI AGGCCT 1 cut(s) 1418
PciI ACATGT 1 cut(s) 1108
PctI GAATGC 1 cut(s) 181
PdmI GAANNNNTTC 1 cut(s) 884
PfeI GAWTC 3 cut(s) 880, 980, 1236
PkrI GCNGC 4 cut(s) 181, 301, 913, 1567
PleI GAGTC 3 cut(s) 102, 272, 628
PpsI GAGTC 3 cut(s) 102, 272, 628
PpuMI RGGWCCY 1 cut(s) 139
PscI ACATGT 1 cut(s) 1108
PshAI GACNNNNGTC 1 cut(s) 112
Psp5II RGGWCCY 1 cut(s) 139
Psp6I CCWGG 1 cut(s) 27
PspGI CCWGG 1 cut(s) 27
PspPI GGNCC 1 cut(s) 139
PspPPI RGGWCCY 1 cut(s) 139
PsuI RGATCY 3 cut(s) 526, 1129, 1199
RsaI GTAC 4 cut(s) 226, 408, 726, 821
RsaNI GTAC 4 cut(s) 225, 407, 725, 820
RseI CAYNNNNRTG 6 cut(s) 272, 308, 537, 720, 1218, 1244
SaqAI TTAA 6 cut(s) 201, 513, 782, 816, 939, 1326
SatI GCNGC 4 cut(s) 180, 300, 912, 1566
Sau3AI GATC 8 cut(s) 54, 63, 130, 526, 1017, 1129, 1199, 1352
Sau96I GGNCC 1 cut(s) 139
SchI GAGTC 3 cut(s) 102, 272, 628
ScrFI CCNGG 1 cut(s) 29
SfaNI GCATC 5 cut(s) 98, 220, 616, 949, 1042
SinI GGWCC 1 cut(s) 139
SmiMI CAYNNNNRTG 6 cut(s) 272, 308, 537, 720, 1218, 1244
SmlI CTYRAG 3 cut(s) 134, 351, 1615
SmoI CTYRAG 3 cut(s) 134, 351, 1615
Sse9I AATT 7 cut(s) 126, 186, 778, 837, 1077, 1323, 1468
SseBI AGGCCT 1 cut(s) 1418
SsiI CCGC 1 cut(s) 1170
SspMI CTAG 1 cut(s) 963
StuI AGGCCT 1 cut(s) 1418
StyD4I CCNGG 1 cut(s) 27
StyI CCWWGG 2 cut(s) 472, 842
TaaI ACNGT 5 cut(s) 219, 719, 902, 1269, 1572
TaqI TCGA 6 cut(s) 33, 367, 1070, 1184, 1433, 1609
TasI AATT 7 cut(s) 126, 186, 778, 837, 1077, 1323, 1468
TatI WGTACW 1 cut(s) 819
TfiI GAWTC 3 cut(s) 880, 980, 1236
Tru1I TTAA 6 cut(s) 201, 513, 782, 816, 939, 1326
Tru9I TTAA 6 cut(s) 201, 513, 782, 816, 939, 1326
TscAI CASTG 2 cut(s) 252, 696
TseFI GTSAC 3 cut(s) 72, 713, 1211
TseI GCWGC 4 cut(s) 179, 299, 911, 1565
Tsp45I GTSAC 3 cut(s) 72, 713, 1211
TspDTI ATGAA 9 cut(s) 521, 588, 698, 819, 1140, 1203, 1236, 1305, 1489
TspGWI ACGGA 1 cut(s) 1445
TspRI CASTG 2 cut(s) 252, 696
VpaK11BI GGWCC 1 cut(s) 139
XapI RAATTY 3 cut(s) 778, 837, 1468
XceI RCATGY 1 cut(s) 1112
XmnI GAANNNNTTC 1 cut(s) 884
XspI CTAG 1 cut(s) 963
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.