MD01G1209400.v1.1

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007729
Physical Location & Seq
Reverse (-)
30352882 .. 30355188
2307 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1209400.v1.1.491

Sequence Viewer

Length: 1272 bp
ATGAGAGACGATGACATATTATGTTGTCAGACCGATTTCTGGGTGTTTATGGTCATATCTTTGGTCCTTGTGGCATTTGCCGGTATTGCATCAGGCCTTGCCTTAGGACTCCTCTCCTTCAGCCAAGTCGATCTTGAAGTCCTCATTAAGTCCGGTCAGCCTCAAGAACAGAAGAATGCAGCGAAGATTCTGCCGCTTGTTAAGAACGAACATCTACTTTTATGTACTCTCCTCATAGGAAAATCACTGGCAATGGAGGCGCTTCCCATTTTCCTGGACTCGATTCTTCCCGTTTGGGCTGCGATTCTTGTGTCAGGCACCCTTGTTCTTGCATTTACGGAGATCATTCCTCAAGCTGTATGTTCTCGATATGGACTAAGTCTAGGCGCGAAAATGTCTCACTTGGTCCGATTGCTTCTTGTGGTTTTCCTCCCCCTGTCGTACCCTTGCAGTAAGCTGCTGGATTGCCTCCTTGGCAAAGGGCATTCGGCGCTCTTAAAACGAGGGGAGCTAAAAACATTGGTGGATCTGCATGCTAATGAGGCTGGGAAAGGTGGAGAGTTATCGCATCATGAAACGACGATTATTGCTGGTGCTCTGGATTTGACTTCCAAGACTGCTAAAGATGCAATGATTCCCATATCTCAAACTTTCTCCCTTGACATCAATTCAAAACTCAACATGGTCTATGATAATTCGCCTTTATACGATGTACTGCAGCAGTTCCAGAAAGGTCATAGTCATATGGTTGCTGTGGTGAAGAGTAACAACAAGGATACTAATTCAAGATCACTTTCAACACTGGCTGAGGGTAGAGGTAATTTAAATTATACTGAAAATAAGGTTTTTGGGCAAAAATTTGTCTTTAGGCTAGTAGAATTCATGGTTGTTAAGTGCGATTCTACAGGATTAAATTTTTCGCATGAACAAAAAGAGCGTTTGAGCATTCCCGTGGACATACCATCACCTCTATATTCAACTGAAACTGCAAATACGACACATAGGCAAGGATTGCGCACGCAAAATGTATCATCTGAAAGTTTAGAATCTCTTCAAGGGGACAAGGAGGTAATCGGCATCATAACAATGGAAGATGTCATGGAGGAACTGTTGCAGGTAACCAAGTTAGTAAATAAATTGATGAACTGTTTTTTTATTTTTTATGGCAATATACATTTGAAAATTAAACTAACCAGTCTATTTATTCTGTCTTCAAGGAAGATATACTGGATGAAACAGATGAATACGTCGATGTTCACAACAAGTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF21

Protein Analysis

424

Amino Acids

46.88

Weight (kDa)

7.61

Isoelectric Point (pI)

31.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNNM PF01595 19 - 188 5.2e-29 Cyclin M transmembrane N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1016
Acc36I ACCTGC 1 cut(s) 1105
AccB1I GGYRCC 1 cut(s) 317
AccII CGCG 1 cut(s) 389
AciI CCGC 1 cut(s) 194
AclWI GGATC 1 cut(s) 534
AcsI RAATTY 3 cut(s) 857, 878, 913
AcuI CTGAAG 1 cut(s) 103
AfaI GTAC 3 cut(s) 226, 443, 714
AfiI CCNNNNNNNGG 1 cut(s) 39
AgsI TTSAA 8 cut(s) 137, 672, 786, 798, 978, 1055, 1180, 1215
AjnI CCWGG 1 cut(s) 273
AluBI AGCT 3 cut(s) 356, 457, 511
AluI AGCT 3 cut(s) 356, 457, 511
Alw21I GWGCWC 1 cut(s) 598
Alw26I GTCTC 1 cut(s) 402
AlwI GGATC 1 cut(s) 534
AoxI GGCC 1 cut(s) 94
ApeKI GCWGC 4 cut(s) 179, 299, 457, 718
ApoI RAATTY 3 cut(s) 857, 878, 913
Asp700I GAANNNNTTC 1 cut(s) 1050
AspLEI GCGC 4 cut(s) 262, 389, 493, 1017
AspS9I GGNCC 2 cut(s) 64, 406
AsuHPI GGTGA 2 cut(s) 769, 957
AvaII GGWCC 2 cut(s) 64, 406
AxyI CCTNAGG 1 cut(s) 103
BanI GGYRCC 1 cut(s) 317
BbsI GAAGAC 1 cut(s) 1203
Bbv12I GWGCWC 1 cut(s) 598
BbvCI CCTCAGC 1 cut(s) 807
BbvI GCAGC 4 cut(s) 191, 286, 444, 730
BccI CCATC 1 cut(s) 970
BcgI CGANNNNNNTGC 2 cut(s) 172, 206
BciT130I CCWGG 1 cut(s) 275
BciVI GTATCC 1 cut(s) 769
BcoDI GTCTC 1 cut(s) 402
BfaI CTAG 2 cut(s) 383, 872
BfmI CTRYAG 2 cut(s) 716, 903
BfoI RGCGCY 2 cut(s) 263, 494
BfuAI ACCTGC 1 cut(s) 1105
BfuI GTATCC 1 cut(s) 769
BglI GCCNNNNNGGC 1 cut(s) 474
BisI GCNGC 5 cut(s) 180, 194, 300, 458, 719
BlsI GCNGC 5 cut(s) 181, 195, 301, 459, 720
Bme1390I CCNGG 1 cut(s) 275
Bme18I GGWCC 2 cut(s) 64, 406
BmgT120I GGNCC 2 cut(s) 64, 406
BmiI GGNNCC 1 cut(s) 319
BmrFI CCNGG 1 cut(s) 275
BmsI GCATC 4 cut(s) 98, 577, 616, 1086
BpiI GAAGAC 1 cut(s) 1203
Bpu10I CCTNAGC 1 cut(s) 807
BpuEI CTTGAG 2 cut(s) 147, 336
BsaJI CCNNGG 2 cut(s) 472, 951
BsaWI WCCGGW 1 cut(s) 152
BsaXI ACNNNNNCTCC 2 cut(s) 1094, 1124
Bsc4I CCNNNNNNNGG 1 cut(s) 39
Bse118I RCCGGY 1 cut(s) 80
Bse1I ACTGG 4 cut(s) 252, 807, 1194, 1232
Bse21I CCTNAGG 1 cut(s) 103
Bse3DI GCAATG 2 cut(s) 258, 636
BseBI CCWGG 1 cut(s) 275
BseDI CCNNGG 2 cut(s) 472, 951
BseGI GGATG 1 cut(s) 1236
BseLI CCNNNNNNNGG 1 cut(s) 39
BseMI GCAATG 2 cut(s) 258, 636
BseMII CTCAG 1 cut(s) 798
BseNI ACTGG 4 cut(s) 252, 807, 1194, 1232
BseRI GAGGAG 2 cut(s) 101, 221
BseXI GCAGC 4 cut(s) 191, 286, 444, 730
BseYI CCCAGC 1 cut(s) 545
Bsh1236I CGCG 1 cut(s) 389
BshFI GGCC 1 cut(s) 96
BshNI GGYRCC 1 cut(s) 317
BsiHKAI GWGCWC 1 cut(s) 598
BsiSI CCGG 2 cut(s) 81, 153
BslFI GGGAC 1 cut(s) 1073
BslI CCNNNNNNNGG 1 cut(s) 39
BsmAI GTCTC 1 cut(s) 402
BsmFI GGGAC 1 cut(s) 1073
BsmI GAATGC 3 cut(s) 181, 484, 945
BsnI GGCC 1 cut(s) 96
Bsp1286I GDGCHC 1 cut(s) 598
Bsp143I GATC 4 cut(s) 130, 342, 526, 788
BspACI CCGC 1 cut(s) 194
BspANI GGCC 1 cut(s) 96
BspCNI CTCAG 1 cut(s) 799
BspFNI CGCG 1 cut(s) 389
BspHI TCATGA 1 cut(s) 571
BspLI GGNNCC 1 cut(s) 319
BspMAI CTGCAG 1 cut(s) 720
BspMI ACCTGC 1 cut(s) 1105
BspPI GGATC 1 cut(s) 534
BspT107I GGYRCC 1 cut(s) 317
BsrDI GCAATG 2 cut(s) 258, 636
BsrFI RCCGGY 1 cut(s) 80
BsrI ACTGG 4 cut(s) 252, 807, 1194, 1232
BssAI RCCGGY 1 cut(s) 80
BssECI CCNNGG 2 cut(s) 472, 951
BssMI GATC 4 cut(s) 130, 342, 526, 788
BssT1I CCWWGG 1 cut(s) 472
Bst2UI CCWGG 1 cut(s) 275
Bst4CI ACNGT 2 cut(s) 1110, 1148
Bst6I CTCTTC 2 cut(s) 755, 1056
BstAPI GCANNNNNTGC 1 cut(s) 1012
BstC8I GCNNGC 2 cut(s) 534, 1019
BstDEI CTNAG 3 cut(s) 103, 377, 807
BstDSI CCRYGG 1 cut(s) 951
BstEII GGTNACC 1 cut(s) 1117
BstF5I GGATG 1 cut(s) 1236
BstFNI CGCG 1 cut(s) 389
BstH2I RGCGCY 2 cut(s) 263, 494
BstHHI GCGC 4 cut(s) 262, 389, 493, 1017
BstKTI GATC 4 cut(s) 133, 345, 529, 791
BstMAI GTCTC 1 cut(s) 402
BstMBI GATC 4 cut(s) 130, 342, 526, 788
BstMWI GCNNNNNNNGC 7 cut(s) 86, 257, 474, 490, 542, 626, 1012
BstNI CCWGG 1 cut(s) 275
BstNSI RCATGY 1 cut(s) 536
BstPI GGTNACC 1 cut(s) 1117
BstSCI CCNGG 1 cut(s) 273
BstSFI CTRYAG 2 cut(s) 716, 903
BstUI CGCG 1 cut(s) 389
BstV1I GCAGC 4 cut(s) 191, 286, 444, 730
BstV2I GAAGAC 1 cut(s) 1203
BstX2I RGATCY 1 cut(s) 526
BstXI CCANNNNNNTGG 1 cut(s) 274
BstYI RGATCY 1 cut(s) 526
Bsu36I CCTNAGG 1 cut(s) 103
BsuI GTATCC 1 cut(s) 769
BsuRI GGCC 1 cut(s) 96
BtgI CCRYGG 1 cut(s) 951
BtsCI GGATG 1 cut(s) 1236
BtsIMutI CAGTG 2 cut(s) 245, 800
BveI ACCTGC 1 cut(s) 1105
Cac8I GCNNGC 2 cut(s) 534, 1019
CciI TCATGA 1 cut(s) 571
CfoI GCGC 4 cut(s) 262, 389, 493, 1017
Cfr10I RCCGGY 1 cut(s) 80
Cfr13I GGNCC 2 cut(s) 64, 406
Csp6I GTAC 3 cut(s) 225, 442, 713
CviAII CATG 6 cut(s) 533, 572, 682, 883, 923, 1099
CviQI GTAC 3 cut(s) 225, 442, 713
DdeI CTNAG 3 cut(s) 103, 377, 807
DpnI GATC 4 cut(s) 132, 344, 528, 790
DpnII GATC 4 cut(s) 130, 342, 526, 788
DraI TTTAAA 1 cut(s) 825
Eam1104I CTCTTC 2 cut(s) 755, 1056
EarI CTCTTC 2 cut(s) 755, 1056
Eco130I CCWWGG 1 cut(s) 472
Eco147I AGGCCT 1 cut(s) 96
Eco47I GGWCC 2 cut(s) 64, 406
Eco57I CTGAAG 1 cut(s) 103
Eco81I CCTNAGG 1 cut(s) 103
Eco91I GGTNACC 1 cut(s) 1117
EcoO65I GGTNACC 1 cut(s) 1117
EcoRI GAATTC 1 cut(s) 878
EcoRII CCWGG 1 cut(s) 273
EcoT14I CCWWGG 1 cut(s) 472
ErhI CCWWGG 1 cut(s) 472
FaeI CATG 6 cut(s) 536, 575, 685, 886, 926, 1102
FalI AAGNNNNNCTT 2 cut(s) 117, 149
FaqI GGGAC 1 cut(s) 1073
FatI CATG 6 cut(s) 532, 571, 681, 882, 922, 1098
FauNDI CATATG 1 cut(s) 744
Fnu4HI GCNGC 5 cut(s) 180, 194, 300, 458, 719
FokI GGATG 1 cut(s) 1243
Fsp4HI GCNGC 5 cut(s) 180, 194, 300, 458, 719
FspBI CTAG 2 cut(s) 383, 872
FspI TGCGCA 1 cut(s) 1016
GlaI GCGC 4 cut(s) 261, 388, 492, 1016
GluI GCNGC 5 cut(s) 180, 194, 300, 458, 719
GsaI CCCAGC 1 cut(s) 549
HaeII RGCGCY 2 cut(s) 263, 494
HaeIII GGCC 1 cut(s) 96
HapII CCGG 2 cut(s) 81, 153
HhaI GCGC 4 cut(s) 262, 389, 493, 1017
Hin1II CATG 6 cut(s) 536, 575, 685, 886, 926, 1102
Hin6I GCGC 4 cut(s) 260, 387, 491, 1015
HinP1I GCGC 4 cut(s) 260, 387, 491, 1015
HinfI GANTC 8 cut(s) 108, 187, 278, 283, 304, 634, 899, 1046
HpaII CCGG 2 cut(s) 81, 153
HphI GGTGA 2 cut(s) 769, 957
Hpy166II GTNNAC 2 cut(s) 955, 1257
Hpy188I TCNGA 3 cut(s) 30, 410, 1036
Hpy188III TCNNGA 7 cut(s) 134, 164, 366, 572, 599, 727, 786
Hpy8I GTNNAC 2 cut(s) 955, 1257
Hpy99I CGWCG 2 cut(s) 583, 1252
HpyAV CCTTC 1 cut(s) 127
HpyCH4III ACNGT 2 cut(s) 1110, 1148
HpyCH4IV ACGT 1 cut(s) 1247
HpyCH4V TGCA 9 cut(s) 89, 179, 332, 450, 532, 629, 718, 989, 1114
HpyF10VI GCNNNNNNNGC 7 cut(s) 86, 257, 474, 490, 542, 626, 1012
HpyF3I CTNAG 3 cut(s) 103, 377, 807
HpySE526I ACGT 1 cut(s) 1247
Hsp92II CATG 6 cut(s) 536, 575, 685, 886, 926, 1102
HspAI GCGC 4 cut(s) 260, 387, 491, 1015
Kzo9I GATC 4 cut(s) 130, 342, 526, 788
LmnI GCTCC 1 cut(s) 508
Lsp1109I GCAGC 4 cut(s) 191, 286, 444, 730
LweI GCATC 4 cut(s) 98, 577, 616, 1086
MaeI CTAG 2 cut(s) 383, 872
MaeII ACGT 1 cut(s) 1247
MaeIII GTNAC 2 cut(s) 764, 1117
MalI GATC 4 cut(s) 132, 344, 528, 790
MboI GATC 4 cut(s) 130, 342, 526, 788
MboII GAAGA 8 cut(s) 184, 196, 278, 772, 1043, 1103, 1203, 1231
MflI RGATCY 1 cut(s) 526
MhlI GDGCHC 1 cut(s) 598
MlyI GAGTC 2 cut(s) 102, 272
MroXI GAANNNNTTC 1 cut(s) 1050
MseI TTAA 7 cut(s) 147, 201, 497, 824, 891, 911, 1185
MslI CAYNNNNRTG 3 cut(s) 537, 950, 1085
MspI CCGG 2 cut(s) 81, 153
MspR9I CCNGG 1 cut(s) 275
Mva1269I GAATGC 3 cut(s) 181, 484, 945
MvaI CCWGG 1 cut(s) 275
MvnI CGCG 1 cut(s) 389
MwoI GCNNNNNNNGC 7 cut(s) 86, 257, 474, 490, 542, 626, 1012
NdeI CATATG 1 cut(s) 744
NdeII GATC 4 cut(s) 130, 342, 526, 788
NlaIII CATG 6 cut(s) 536, 575, 685, 886, 926, 1102
NlaIV GGNNCC 1 cut(s) 319
NsbI TGCGCA 1 cut(s) 1016
NspI RCATGY 1 cut(s) 536
PaeI GCATGC 1 cut(s) 536
PagI TCATGA 1 cut(s) 571
PceI AGGCCT 1 cut(s) 96
PctI GAATGC 3 cut(s) 181, 484, 945
PdmI GAANNNNTTC 1 cut(s) 1050
PfeI GAWTC 6 cut(s) 187, 283, 304, 634, 899, 1046
PflFI GACNNNGTC 1 cut(s) 378
PfoI TCCNGGA 1 cut(s) 273
PkrI GCNGC 5 cut(s) 181, 195, 301, 459, 720
PleI GAGTC 2 cut(s) 102, 272
PpsI GAGTC 2 cut(s) 102, 272
Psp6I CCWGG 1 cut(s) 273
PspEI GGTNACC 1 cut(s) 1117
PspFI CCCAGC 1 cut(s) 545
PspGI CCWGG 1 cut(s) 273
PspN4I GGNNCC 1 cut(s) 319
PspPI GGNCC 2 cut(s) 64, 406
PstI CTGCAG 1 cut(s) 720
PsuI RGATCY 1 cut(s) 526
PsyI GACNNNGTC 1 cut(s) 378
RsaI GTAC 3 cut(s) 226, 443, 714
RsaNI GTAC 3 cut(s) 225, 442, 713
RseI CAYNNNNRTG 3 cut(s) 537, 950, 1085
SaqAI TTAA 7 cut(s) 147, 201, 497, 824, 891, 911, 1185
SatI GCNGC 5 cut(s) 180, 194, 300, 458, 719
Sau3AI GATC 4 cut(s) 130, 342, 526, 788
Sau96I GGNCC 2 cut(s) 64, 406
SchI GAGTC 2 cut(s) 102, 272
ScrFI CCNGG 1 cut(s) 275
SduI GDGCHC 1 cut(s) 598
SfaNI GCATC 4 cut(s) 98, 577, 616, 1086
SfcI CTRYAG 2 cut(s) 716, 903
SinI GGWCC 2 cut(s) 64, 406
SmiI ATTTAAAT 1 cut(s) 825
SmiMI CAYNNNNRTG 3 cut(s) 537, 950, 1085
SmlI CTYRAG 2 cut(s) 162, 351
SmoI CTYRAG 2 cut(s) 162, 351
SphI GCATGC 1 cut(s) 536
SseBI AGGCCT 1 cut(s) 96
SsiI CCGC 1 cut(s) 194
SspMI CTAG 2 cut(s) 383, 872
StuI AGGCCT 1 cut(s) 96
StyD4I CCNGG 1 cut(s) 273
StyI CCWWGG 1 cut(s) 472
SwaI ATTTAAAT 1 cut(s) 825
TaaI ACNGT 2 cut(s) 1110, 1148
TaiI ACGT 1 cut(s) 1250
TaqI TCGA 4 cut(s) 129, 281, 367, 1250
TaqII GACCGA 1 cut(s) 47
TatI WGTACW 2 cut(s) 224, 712
TauI GCSGC 1 cut(s) 196
TfiI GAWTC 6 cut(s) 187, 283, 304, 634, 899, 1046
Tru1I TTAA 7 cut(s) 147, 201, 497, 824, 891, 911, 1185
Tru9I TTAA 7 cut(s) 147, 201, 497, 824, 891, 911, 1185
TscAI CASTG 2 cut(s) 252, 807
TseI GCWGC 4 cut(s) 179, 299, 457, 718
TspDTI ATGAA 6 cut(s) 588, 871, 939, 1157, 1247, 1256
TspGWI ACGGA 1 cut(s) 353
TspRI CASTG 2 cut(s) 252, 807
Tth111I GACNNNGTC 1 cut(s) 378
VpaK11BI GGWCC 2 cut(s) 64, 406
XapI RAATTY 3 cut(s) 857, 878, 913
XceI RCATGY 1 cut(s) 536
XmnI GAANNNNTTC 1 cut(s) 1050
XspI CTAG 2 cut(s) 383, 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.