Rorug01G0409200

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0007729
Physical Location & Seq
Forward (+)
51488517 .. 51490508
1992 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0409200.1

Sequence Viewer

Length: 1743 bp
ATGTGTTCACTATTATGTCTTGGTGAGGATGATGTTCGTGTTGTTGGAGTATGCGGAATGCCTGGTATAGGGAAAACAACCATTGCTAGAGCTGTTTATGACGAAATTGTTTGTCAATTTGAACACTATTGCTTTCTTGAAAATGTCAAGGATGGTTTCAAGAACAATGGTGCAATACACATGCAGGAAGAACTCCTATCTAGAATATTTGAGAAAAGAGTGCGCTGTCTAGGCACTTTGAGTAAAGGATCCAAGATGATAATGGAAAGGCTGAGTAAGAAAAAAGTTCTGCTTGTTCTTGATGATGTAGAAAACTTTGCACAAATTGAAGCGTTACTTGGAAACCAGCATTCATTTGGTGGTGGAAGTAGAATCATTGTAACCACTAGAGATATACAATCACTCAGTGGAGTTGATGCGAGATATAGTCCCATGTTTCTAAGTGATGATGAAGCTCTTGAGCTCTTTATGCAGTATGCCTTCAGAACAAACCAACCTACAAGAGAGTATGATCCTCTCTCTAGGCGTGCCATAGAATATGCTCATGGTCTGCCTCTAGCACTCAAAGTTTTGGGAGCTTTTCTTGATAACAAAAGTATATGTGAGTGGGAAGATGAGATAGAGAAAATAAAGGAAATCCCACATATAGAAATTCAGGGTGTGCTTAAAAAAAGTTTTGATGGCCTGGATCCTTCACAGAAGGACATATTCCTAGATAATGCGTGCTTCTTTAGAGGAATGGACAAAGGCTATGTGACCAAAATTCTGGAAAATGACAATGGGTTGGAAATGCATGATTTAATAAAGGAGATGGGTTGGGATATTATCCGCCAACAGTCTATTAAAGAGCCTGGGAAGCGCAGTAGGTTGTGGGTTTATGAAGATGTTGCTCGTGTATTAACTCAAAATATGGCTACAGATGCAGTTGAATGCATAATGCTAGACTTGTCGAAGTCAAAAGACGTCTACATAGATGCTGAAGCTTTTGTTAGAATGACGAAATTAAGACTGCTCAGAATCGGTTATAACCACTCCATAGACTTGAATGCGAAAGATTTTCAATCGAATCAGTCAGACCATCCAAGAGGTGAGTGTAAACAACATGTAAGCGGGGACGTAAAGTTTCTTTCTCATGACTTGAGGTATCTCATGTGGTATGGATGCCCCCTGAAGTCTTTGCCATCCAATTTTCACCCGAAGAATCTTGTTGACCTTGACATGCGTTGTAGCCATATTGAACAACTTTGGGAAGAAATCAAGCCCTTCAAAAAGTTGAAATTCATCAATTTGAGTCATTCTCATTACCTTACCACAACTCCCAACTTCACTGAAGCAACAAATGTTGAAACACTAGTTCTTGAAGGTTGTTCGAGTTTGCTTGATGTCAACTCAACCATTTCAGCTCTTAAAAACCTTGTATTCTTGTGCCTAAGAGGATGCAAAGAACTTAAGAGTCTTCCAAGCAGCATTTGTTTGAAGTCTCTTAAAACCCTTGATCTTTCTGGGTGCTCAAGTCTCGTAAAGTTTCCAGAGATTTCAGGGATCATGGAGGACCTATCAGAAATTTATCTAAATGATACTGCAATTGAAGAATTGCCATCATCGATTGAACGACTTCAGGGGCTTGTATTACTACATTTGAGAAACTGTAGAAGCCTTGTCCATCTTCCAGACAATATATGTAAGTTGGTATATCTTAAAGATCTCACTCTCTCTGGGTGCTCAGCTCTATCATTTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF21

Protein Analysis

580

Amino Acids

65.76

Weight (kDa)

5.87

Isoelectric Point (pI)

41.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 9 - 164 1.5e-18 NB-ARC domain
LRR_3 PF07725 401 - 420 1e-06 Leucine Rich Repeat
LRR_14 PF23598 486 - 573 8.6e-07 Leucine-rich repeat region
LRR_13 PF23286 492 - 577 6.2e-12 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1026
AatII GACGTC 1 cut(s) 966
AccI GTMKAC 1 cut(s) 966
AciI CCGC 3 cut(s) 54, 829, 1110
AclWI GGATC 6 cut(s) 243, 256, 506, 683, 696, 1550
AcsI RAATTY 4 cut(s) 651, 762, 1277, 1563
AcuI CTGAAG 5 cut(s) 466, 999, 1190, 1350, 1601
AcyI GRCGYC 1 cut(s) 963
AdeI CACNNNGTG 1 cut(s) 407
AfiI CCNNNNNNNGG 1 cut(s) 68
AflII CTTAAG 1 cut(s) 1448
AflIII ACRYGT 1 cut(s) 1102
AhlI ACTAGT 1 cut(s) 1351
AjnI CCWGG 3 cut(s) 61, 684, 850
AjuI GAANNNNNNNTTGG 2 cut(s) 321, 353
AluBI AGCT 7 cut(s) 92, 455, 463, 578, 983, 1403, 1727
AluI AGCT 7 cut(s) 92, 455, 463, 578, 983, 1403, 1727
Alw21I GWGCWC 3 cut(s) 465, 1511, 1724
Alw26I GTCTC 2 cut(s) 1485, 1520
AlwI GGATC 6 cut(s) 243, 256, 506, 683, 696, 1550
AoxI GGCC 1 cut(s) 682
ApeKI GCWGC 1 cut(s) 1464
ApoI RAATTY 4 cut(s) 651, 762, 1277, 1563
Asp700I GAANNNNTTC 1 cut(s) 1614
AspLEI GCGC 2 cut(s) 225, 861
AspS9I GGNCC 1 cut(s) 1552
AsuHPI GGTGA 3 cut(s) 35, 1100, 1184
AvaII GGWCC 1 cut(s) 1552
BamHI GGATCC 2 cut(s) 248, 688
BanII GRGCYC 1 cut(s) 465
BauI CACGAG 1 cut(s) 891
BbsI GAAGAC 1 cut(s) 1448
Bbv12I GWGCWC 3 cut(s) 465, 1511, 1724
BbvI GCAGC 1 cut(s) 1476
BccI CCATC 7 cut(s) 146, 674, 805, 1086, 1189, 1606, 1671
BciT130I CCWGG 3 cut(s) 63, 686, 852
BcoDI GTCTC 2 cut(s) 1485, 1520
BcuI ACTAGT 1 cut(s) 1351
BfaI CTAG 9 cut(s) 87, 201, 230, 387, 522, 557, 713, 941, 1352
BfmI CTRYAG 2 cut(s) 915, 1648
BfrI CTTAAG 1 cut(s) 1448
BglII AGATCT 1 cut(s) 1702
BisI GCNGC 1 cut(s) 1465
BlpI GCTNAGC 1 cut(s) 1723
BlsI GCNGC 1 cut(s) 1466
Bme1390I CCNGG 3 cut(s) 63, 686, 852
Bme18I GGWCC 1 cut(s) 1552
BmgT120I GGNCC 1 cut(s) 1552
BmiI GGNNCC 2 cut(s) 250, 690
BmrFI CCNGG 3 cut(s) 63, 686, 852
BmsI GCATC 5 cut(s) 406, 910, 964, 1151, 1427
BpiI GAAGAC 1 cut(s) 1448
BplI GAGNNNNNCTC 2 cut(s) 1282, 1314
Bpu1102I GCTNAGC 1 cut(s) 1723
BpuEI CTTGAG 3 cut(s) 479, 1159, 1495
Bsa29I ATCGAT 1 cut(s) 1604
BsaHI GRCGYC 1 cut(s) 963
BsaJI CCNNGG 1 cut(s) 851
BsaXI ACNNNNNCTCC 2 cut(s) 1300, 1330
Bsc4I CCNNNNNNNGG 1 cut(s) 68
Bse3DI GCAATG 1 cut(s) 81
BseBI CCWGG 3 cut(s) 63, 686, 852
BseCI ATCGAT 1 cut(s) 1604
BseDI CCNNGG 1 cut(s) 851
BseGI GGATG 6 cut(s) 34, 157, 1078, 1166, 1181, 1442
BseLI CCNNNNNNNGG 1 cut(s) 68
BseMI GCAATG 1 cut(s) 81
BseMII CTCAG 4 cut(s) 263, 418, 1027, 1737
BseXI GCAGC 1 cut(s) 1476
BshFI GGCC 1 cut(s) 684
BshVI ATCGAT 1 cut(s) 1604
BsiHKAI GWGCWC 3 cut(s) 465, 1511, 1724
BslFI GGGAC 2 cut(s) 414, 1127
BslI CCNNNNNNNGG 1 cut(s) 68
BsmAI GTCTC 2 cut(s) 1485, 1520
BsmFI GGGAC 2 cut(s) 414, 1127
BsmI GAATGC 4 cut(s) 63, 349, 935, 1051
BsnI GGCC 1 cut(s) 684
Bsp1286I GDGCHC 3 cut(s) 465, 1511, 1724
Bsp143I GATC 6 cut(s) 248, 511, 688, 1495, 1542, 1702
Bsp1720I GCTNAGC 1 cut(s) 1723
BspACI CCGC 3 cut(s) 54, 829, 1110
BspANI GGCC 1 cut(s) 684
BspCNI CTCAG 4 cut(s) 264, 417, 1026, 1736
BspDI ATCGAT 1 cut(s) 1604
BspHI TCATGA 1 cut(s) 1132
BspLI GGNNCC 2 cut(s) 250, 690
BspPI GGATC 6 cut(s) 243, 256, 506, 683, 696, 1550
BspTI CTTAAG 1 cut(s) 1448
BsrDI GCAATG 1 cut(s) 81
BssECI CCNNGG 1 cut(s) 851
BssMI GATC 6 cut(s) 248, 511, 688, 1495, 1542, 1702
BssNI GRCGYC 1 cut(s) 963
BssSI CACGAG 1 cut(s) 891
Bst2BI CACGAG 1 cut(s) 891
Bst2UI CCWGG 3 cut(s) 63, 686, 852
Bst4CI ACNGT 2 cut(s) 837, 1649
BstACI GRCGYC 1 cut(s) 963
BstAFI CTTAAG 1 cut(s) 1448
BstC8I GCNNGC 2 cut(s) 528, 724
BstDEI CTNAG 6 cut(s) 272, 404, 440, 1013, 1430, 1723
BstENI CCTNNNNNAGG 1 cut(s) 66
BstF5I GGATG 6 cut(s) 34, 157, 1078, 1166, 1181, 1442
BstHHI GCGC 2 cut(s) 225, 861
BstKTI GATC 6 cut(s) 251, 514, 691, 1498, 1545, 1705
BstMAI GTCTC 2 cut(s) 1485, 1520
BstMBI GATC 6 cut(s) 248, 511, 688, 1495, 1542, 1702
BstMWI GCNNNNNNNGC 4 cut(s) 231, 469, 856, 920
BstNI CCWGG 3 cut(s) 63, 686, 852
BstNSI RCATGY 3 cut(s) 184, 1106, 1222
BstSCI CCNGG 3 cut(s) 61, 684, 850
BstSFI CTRYAG 2 cut(s) 915, 1648
BstV1I GCAGC 1 cut(s) 1476
BstV2I GAAGAC 1 cut(s) 1448
BstX2I RGATCY 3 cut(s) 248, 688, 1702
BstXI CCANNNNNNTGG 1 cut(s) 766
BstYI RGATCY 3 cut(s) 248, 688, 1702
Bsu15I ATCGAT 1 cut(s) 1604
BsuRI GGCC 1 cut(s) 684
BsuTUI ATCGAT 1 cut(s) 1604
BtsCI GGATG 6 cut(s) 34, 157, 1078, 1166, 1181, 1442
BtsIMutI CAGTG 2 cut(s) 412, 1326
Cac8I GCNNGC 2 cut(s) 528, 724
CciI TCATGA 1 cut(s) 1132
CfoI GCGC 2 cut(s) 225, 861
Cfr13I GGNCC 1 cut(s) 1552
ClaI ATCGAT 1 cut(s) 1604
CviAII CATG 9 cut(s) 181, 433, 545, 794, 1103, 1133, 1150, 1219, 1546
DdeI CTNAG 6 cut(s) 272, 404, 440, 1013, 1430, 1723
DpnI GATC 6 cut(s) 250, 513, 690, 1497, 1544, 1704
DpnII GATC 6 cut(s) 248, 511, 688, 1495, 1542, 1702
DraIII CACNNNGTG 1 cut(s) 407
EciI GGCGGA 1 cut(s) 818
Ecl136II GAGCTC 1 cut(s) 463
Eco24I GRGCYC 1 cut(s) 465
Eco47I GGWCC 1 cut(s) 1552
Eco53kI GAGCTC 1 cut(s) 463
Eco57I CTGAAG 5 cut(s) 466, 999, 1190, 1350, 1601
EcoICRI GAGCTC 1 cut(s) 463
EcoNI CCTNNNNNAGG 1 cut(s) 66
EcoO109I RGGNCCY 1 cut(s) 1552
EcoRII CCWGG 3 cut(s) 61, 684, 850
EcoT22I ATGCAT 2 cut(s) 795, 935
EcoT38I GRGCYC 1 cut(s) 465
FaeI CATG 9 cut(s) 184, 436, 548, 797, 1106, 1136, 1153, 1222, 1549
FalI AAGNNNNNCTT 4 cut(s) 276, 308, 321, 353
FaqI GGGAC 2 cut(s) 414, 1127
FatI CATG 9 cut(s) 180, 432, 544, 793, 1102, 1132, 1149, 1218, 1545
FauI CCCGC 1 cut(s) 1103
FblI GTMKAC 1 cut(s) 966
Fnu4HI GCNGC 1 cut(s) 1465
FokI GGATG 6 cut(s) 41, 164, 1065, 1168, 1173, 1449
FriOI GRGCYC 1 cut(s) 465
Fsp4HI GCNGC 1 cut(s) 1465
FspBI CTAG 9 cut(s) 87, 201, 230, 387, 522, 557, 713, 941, 1352
GlaI GCGC 2 cut(s) 224, 860
GluI GCNGC 1 cut(s) 1465
HaeIII GGCC 1 cut(s) 684
HhaI GCGC 2 cut(s) 225, 861
Hin1I GRCGYC 1 cut(s) 963
Hin1II CATG 9 cut(s) 184, 436, 548, 797, 1106, 1136, 1153, 1222, 1549
Hin6I GCGC 2 cut(s) 223, 859
HinP1I GCGC 2 cut(s) 223, 859
HincII GTYRAC 2 cut(s) 1210, 1387
HindII GTYRAC 2 cut(s) 1210, 1387
HindIII AAGCTT 1 cut(s) 981
HinfI GANTC 6 cut(s) 372, 1017, 1066, 1201, 1291, 1453
HphI GGTGA 3 cut(s) 35, 1100, 1184
Hpy166II GTNNAC 5 cut(s) 8, 967, 1097, 1210, 1387
Hpy188I TCNGA 4 cut(s) 485, 1016, 1075, 1561
Hpy8I GTNNAC 5 cut(s) 8, 967, 1097, 1210, 1387
HpyAV CCTTC 5 cut(s) 490, 694, 702, 1273, 1355
HpyCH4III ACNGT 2 cut(s) 837, 1649
HpyCH4IV ACGT 2 cut(s) 963, 1116
HpyCH4V TGCA 9 cut(s) 173, 184, 320, 472, 793, 923, 933, 1440, 1583
HpyF10VI GCNNNNNNNGC 4 cut(s) 231, 469, 856, 920
HpyF3I CTNAG 6 cut(s) 272, 404, 440, 1013, 1430, 1723
HpySE526I ACGT 2 cut(s) 963, 1116
Hsp92I GRCGYC 1 cut(s) 963
Hsp92II CATG 9 cut(s) 184, 436, 548, 797, 1106, 1136, 1153, 1222, 1549
HspAI GCGC 2 cut(s) 223, 859
Kzo9I GATC 6 cut(s) 248, 511, 688, 1495, 1542, 1702
LmnI GCTCC 1 cut(s) 575
Lsp1109I GCAGC 1 cut(s) 1476
LweI GCATC 5 cut(s) 406, 910, 964, 1151, 1427
MaeI CTAG 9 cut(s) 87, 201, 230, 387, 522, 557, 713, 941, 1352
MaeII ACGT 2 cut(s) 963, 1116
MaeIII GTNAC 3 cut(s) 333, 379, 754
MalI GATC 6 cut(s) 250, 513, 690, 1497, 1544, 1704
MboI GATC 6 cut(s) 248, 511, 688, 1495, 1542, 1702
MboII GAAGA 8 cut(s) 200, 623, 893, 1210, 1262, 1448, 1601, 1658
MfeI CAATTG 1 cut(s) 1584
MflI RGATCY 3 cut(s) 248, 688, 1702
MhlI GDGCHC 3 cut(s) 465, 1511, 1724
MlyI GAGTC 2 cut(s) 1300, 1462
MmeI TCCRAC 2 cut(s) 25, 765
MnlI CCTC 8 cut(s) 19, 525, 564, 728, 1079, 1134, 1427, 1543
Mph1103I ATGCAT 2 cut(s) 795, 935
MroXI GAANNNNTTC 1 cut(s) 1614
MseI TTAA 9 cut(s) 666, 800, 843, 899, 1004, 1407, 1449, 1485, 1698
MslI CAYNNNNRTG 1 cut(s) 13
MspCI CTTAAG 1 cut(s) 1448
MspR9I CCNGG 3 cut(s) 63, 686, 852
MunI CAATTG 1 cut(s) 1584
Mva1269I GAATGC 4 cut(s) 63, 349, 935, 1051
MvaI CCWGG 3 cut(s) 63, 686, 852
MwoI GCNNNNNNNGC 4 cut(s) 231, 469, 856, 920
NdeII GATC 6 cut(s) 248, 511, 688, 1495, 1542, 1702
NlaIII CATG 9 cut(s) 184, 436, 548, 797, 1106, 1136, 1153, 1222, 1549
NlaIV GGNNCC 2 cut(s) 250, 690
NmuCI GTSAC 1 cut(s) 754
NsiI ATGCAT 2 cut(s) 795, 935
NspI RCATGY 3 cut(s) 184, 1106, 1222
PagI TCATGA 1 cut(s) 1132
PciI ACATGT 1 cut(s) 1102
PctI GAATGC 4 cut(s) 63, 349, 935, 1051
PdmI GAANNNNTTC 1 cut(s) 1614
PfeI GAWTC 4 cut(s) 372, 1017, 1066, 1201
PkrI GCNGC 1 cut(s) 1466
PleI GAGTC 2 cut(s) 1299, 1461
PpsI GAGTC 2 cut(s) 1299, 1461
PpuMI RGGWCCY 1 cut(s) 1552
PscI ACATGT 1 cut(s) 1102
PsiI TTATAA 1 cut(s) 1026
Psp124BI GAGCTC 1 cut(s) 465
Psp5II RGGWCCY 1 cut(s) 1552
Psp6I CCWGG 3 cut(s) 61, 684, 850
PspGI CCWGG 3 cut(s) 61, 684, 850
PspN4I GGNNCC 2 cut(s) 250, 690
PspPI GGNCC 1 cut(s) 1552
PspPPI RGGWCCY 1 cut(s) 1552
PsuI RGATCY 3 cut(s) 248, 688, 1702
RseI CAYNNNNRTG 1 cut(s) 13
SacI GAGCTC 1 cut(s) 465
SaqAI TTAA 9 cut(s) 666, 800, 843, 899, 1004, 1407, 1449, 1485, 1698
SatI GCNGC 1 cut(s) 1465
Sau3AI GATC 6 cut(s) 248, 511, 688, 1495, 1542, 1702
Sau96I GGNCC 1 cut(s) 1552
SchI GAGTC 2 cut(s) 1300, 1462
ScrFI CCNGG 3 cut(s) 63, 686, 852
SduI GDGCHC 3 cut(s) 465, 1511, 1724
SfaNI GCATC 5 cut(s) 406, 910, 964, 1151, 1427
SfcI CTRYAG 2 cut(s) 915, 1648
SinI GGWCC 1 cut(s) 1552
SmiMI CAYNNNNRTG 1 cut(s) 13
SmlI CTYRAG 4 cut(s) 458, 1138, 1448, 1510
SmoI CTYRAG 4 cut(s) 458, 1138, 1448, 1510
SpeI ACTAGT 1 cut(s) 1351
SsiI CCGC 3 cut(s) 54, 829, 1110
SspI AATATT 1 cut(s) 207
SspMI CTAG 9 cut(s) 87, 201, 230, 387, 522, 557, 713, 941, 1352
SstI GAGCTC 1 cut(s) 465
StyD4I CCNGG 3 cut(s) 61, 684, 850
TaaI ACNGT 2 cut(s) 837, 1649
TaiI ACGT 2 cut(s) 966, 1119
TaqI TCGA 4 cut(s) 950, 1064, 1370, 1604
TfiI GAWTC 4 cut(s) 372, 1017, 1066, 1201
Tru1I TTAA 9 cut(s) 666, 800, 843, 899, 1004, 1407, 1449, 1485, 1698
Tru9I TTAA 9 cut(s) 666, 800, 843, 899, 1004, 1407, 1449, 1485, 1698
TscAI CASTG 2 cut(s) 412, 1333
TseFI GTSAC 1 cut(s) 754
TseI GCWGC 1 cut(s) 1464
Tsp45I GTSAC 1 cut(s) 754
TspDTI ATGAA 4 cut(s) 342, 465, 894, 1270
TspRI CASTG 2 cut(s) 412, 1333
Vha464I CTTAAG 1 cut(s) 1448
VpaK11BI GGWCC 1 cut(s) 1552
XagI CCTNNNNNAGG 1 cut(s) 66
XapI RAATTY 4 cut(s) 651, 762, 1277, 1563
XbaI TCTAGA 1 cut(s) 200
XceI RCATGY 3 cut(s) 184, 1106, 1222
XcmI CCANNNNNNNNNTGG 2 cut(s) 259, 353
XmiI GTMKAC 1 cut(s) 966
XmnI GAANNNNTTC 1 cut(s) 1614
XspI CTAG 9 cut(s) 87, 201, 230, 387, 522, 557, 713, 941, 1352
ZraI GACGTC 1 cut(s) 964
Zsp2I ATGCAT 2 cut(s) 795, 935
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.