Rroxscaffold_4G00280430

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0007729
Physical Location & Seq
Forward (+)
2993818 .. 3003972
10155 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00280430.1

Sequence Viewer

Length: 1770 bp
ATGGGAGAGGAGAGAAAAAGATTATCCAAAGAGGACTACATCGATCTTGATATAAAGAAGAAGATGAGGATGACTGGGGTTGTGGAGTTTGTGGCATGTTGGGACGAGCATGCTGTTCAAATGAAGGAGCAGCTGCTGTTCTCAAGAGAGGATGATGTTCCGTGTTGCCAGGTCGATTTCTGGGTGTTTATAGTGATTAGCTTGATCCTTGCGTCACTTGCTGGTATTGCATCAGGCCTTGCATTAGGACTCTTGTCCTTCAGCAAAGTTGATCTTGAGGTTCTCATTAGGTCCGGTCAACCCAAAGACCAGAAGAACGCAGCAACGATTCTGCCACTTGTTAAGAATGAGCATCTACTGTTATGTACTCTCCTCATATGCAAATCATTGGCCTTGGAGGCACTTCCCATTTTCGTGGACTCAATTCTCCCAGTTTGGGCTGCCATTCTTGTGTCTGGCACGATTGTAGTTGCAGTTACAGAGCTGTTGGATTGCCTCCTTGGAGAAGGGCATTCTGCTCTCCTAAGACGAGCAGAGCTAAAAACATTGGTGGATCTGCATGCAAATGAGGCAGGGATAGGTGGAGAGTTGTCGCATCATGAAACTACGGTCATTGGTGGTGCTTTGGATTTGACGCGAAAGACTGCTGAAGATGCAATGACTCCAATATCAGAAACTTTTTCCCTTGACATAAACTCCAAACTTGACATGAATACACTGGGTTCAATAATGAGCAGAGGTCATAGTCGTATACCAATCTATTCAGGAAACCCAAACAATATCATTGGCCTTATTTTGGCAAAGAATTTGATCTTCTGTCGCCCTGAAGATGAAACCCCAGTTAAGTACATGACAATCAGAAAAATTCCAAGGGTTTATGGCAATTGGCCTTTATATGATATCCTGAATCAGTTCCGGAAAGGCTACAGTCACATGGCTGCTGTGGTGAGGAATAAAAAGGACGTTAATGGACAGAAGGAAAAAGATGCTAGGAGTTCAAAATTAGATTCAACACGAGCGGAGAGCAAAGGTATTACATTGGGTTTGATTAGTGAAAACCAGTTTTCAGATATTACAGGTTTATTAAATTCTCCGATCAATGGGAAAGAGCATCTGAGTAACACCATAGACACATCCCCTGTTTACTCGAGTACTGATACAGAATACTGCACTCCGCTATCAGAGCATGTCATGGAGCAAGATGAAGATCTTAAGCAGATGCATAACAAGCGCCATAAAAAGCAGGGAGGTGGAAATGTATCAAATGAAAGTGTAGGATCTATTCAAAGTCACTTGGACGAGGAGGTAATTGGTATCATAACAATGGAGGATGTTATGGAGGAACTGCTACAGGAAGACATACTGGATGAAACAGATGAGTATGTGGATGTGCATAACAAAATCAAAATCAACCTGCTACCTTCAAGAAGATCATCACCCTCAAAATCTCCTAGAACAAAGTCTCTATCTCAAATTTATTGGAGAACTCCGGAGCCCTCTCCCCTGCCTTCGAAATACAATAGTCCTATACTCCGTTCTCCCATTAATCCCGCATACATTCAACCACCTCCTTTCCTCATCACCAGACCTACTTTGTATCCTTCCCCTGCTAAATCATTACTCAATATCAGTTCTCCACAAGCTGGACCATTCGCAGGCCCTATTCGCGACTCACCATCCTCACATCGAATCAAACTCACATTACAATGTAACCAGCTACTAGCTACTCTTCCGTTTCGACCTTCGACGAAGTTGCTTCGGATGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF21

Protein Analysis

589

Amino Acids

65.4

Weight (kDa)

6.24

Isoelectric Point (pI)

53.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNNM PF01595 66 - 159 7.4e-09 Cyclin M transmembrane N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1422
AccB7I CCANNNNNTGG 1 cut(s) 1643
AccBSI CCGCTC 1 cut(s) 1019
AccI GTMKAC 1 cut(s) 751
AccII CGCG 2 cut(s) 637, 1668
AccIII TCCGGA 2 cut(s) 915, 1489
AciI CCGC 3 cut(s) 1019, 1175, 1551
AclWI GGATC 3 cut(s) 199, 561, 1285
AcsI RAATTY 4 cut(s) 805, 864, 1087, 1473
AcuI CTGAAG 3 cut(s) 244, 669, 846
AfaI GTAC 3 cut(s) 367, 848, 1153
AfiI CCNNNNNNNGG 5 cut(s) 436, 796, 1100, 1643, 1655
AflII CTTAAG 1 cut(s) 1211
AgsI TTSAA 7 cut(s) 119, 726, 999, 1011, 1286, 1425, 1562
AjnI CCWGG 1 cut(s) 168
AluBI AGCT 8 cut(s) 133, 201, 484, 538, 1643, 1717, 1724, 1767
AluI AGCT 8 cut(s) 133, 201, 484, 538, 1643, 1717, 1724, 1767
Alw26I GTCTC 1 cut(s) 1467
AlwI GGATC 3 cut(s) 199, 561, 1285
AlwNI CAGNNNCTG 1 cut(s) 136
Ama87I CYCGRG 1 cut(s) 1147
Aor13HI TCCGGA 2 cut(s) 915, 1489
AoxI GGCC 5 cut(s) 235, 390, 787, 887, 1657
ApeKI GCWGC 5 cut(s) 130, 133, 320, 440, 938
ApoI RAATTY 4 cut(s) 805, 864, 1087, 1473
AseI ATTAAT 1 cut(s) 1545
Asp700I GAANNNNTTC 1 cut(s) 911
AspLEI GCGC 1 cut(s) 1233
AspS9I GGNCC 3 cut(s) 291, 1646, 1658
AsuHPI GGTGA 4 cut(s) 958, 1428, 1573, 1665
AsuII TTCGAA 1 cut(s) 1511
AvaI CYCGRG 1 cut(s) 1147
AvaII GGWCC 2 cut(s) 291, 1646
BanII GRGCYC 1 cut(s) 1497
BauI CACGAG 1 cut(s) 1014
BbsI GAAGAC 1 cut(s) 1362
BbvI GCAGC 5 cut(s) 120, 142, 332, 427, 925
BccI CCATC 1 cut(s) 1684
BcgI CGANNNNNNTGC 2 cut(s) 1735, 1769
BciT130I CCWGG 1 cut(s) 170
BciVI GTATCC 1 cut(s) 1608
BcoDI GTCTC 1 cut(s) 1467
BfaI CTAG 4 cut(s) 990, 1452, 1721, 1768
BfmI CTRYAG 2 cut(s) 925, 1349
BfoI RGCGCY 1 cut(s) 1234
BfrI CTTAAG 1 cut(s) 1211
BfuAI ACCTGC 1 cut(s) 1422
BfuI GTATCC 1 cut(s) 1608
BglI GCCNNNNNGGC 1 cut(s) 398
BglII AGATCT 1 cut(s) 1207
BisI GCNGC 5 cut(s) 131, 134, 321, 441, 939
BlsI GCNGC 5 cut(s) 132, 135, 322, 442, 940
BmcAI AGTACT 1 cut(s) 1153
Bme1390I CCNGG 1 cut(s) 170
Bme18I GGWCC 2 cut(s) 291, 1646
BmeT110I CYCGRG 1 cut(s) 1147
BmgT120I GGNCC 3 cut(s) 291, 1646, 1658
BmiI GGNNCC 1 cut(s) 1494
BmrFI CCNGG 1 cut(s) 170
BmrI ACTGGG 4 cut(s) 84, 425, 728, 833
BmsI GCATC 7 cut(s) 239, 361, 604, 643, 976, 1120, 1209
BmuI ACTGGG 4 cut(s) 84, 425, 728, 833
BoxI GACNNNNGTC 1 cut(s) 253
BpiI GAAGAC 1 cut(s) 1362
Bpu14I TTCGAA 1 cut(s) 1511
BpuEI CTTGAG 2 cut(s) 127, 296
Bsa29I ATCGAT 1 cut(s) 42
BsaBI GATNNNNATC 1 cut(s) 1206
BsaJI CCNNGG 3 cut(s) 393, 499, 869
BsaWI WCCGGW 3 cut(s) 293, 915, 1489
BsaXI ACNNNNNCTCC 2 cut(s) 680, 710
Bsc4I CCNNNNNNNGG 5 cut(s) 436, 796, 1100, 1643, 1655
Bse1I ACTGG 6 cut(s) 79, 431, 723, 839, 1060, 1368
Bse3DI GCAATG 1 cut(s) 663
Bse8I GATNNNNATC 1 cut(s) 1206
BseAI TCCGGA 2 cut(s) 915, 1489
BseBI CCWGG 1 cut(s) 170
BseCI ATCGAT 1 cut(s) 42
BseDI CCNNGG 3 cut(s) 393, 499, 869
BseGI GGATG 8 cut(s) 75, 157, 1133, 1336, 1372, 1393, 1676, 1767
BseJI GATNNNNATC 1 cut(s) 1206
BseLI CCNNNNNNNGG 5 cut(s) 436, 796, 1100, 1643, 1655
BseMI GCAATG 1 cut(s) 663
BseMII CTCAG 1 cut(s) 1106
BseNI ACTGG 6 cut(s) 79, 431, 723, 839, 1060, 1368
BseRI GAGGAG 3 cut(s) 23, 362, 1316
BseXI GCAGC 5 cut(s) 120, 142, 332, 427, 925
BsgI GTGCAG 1 cut(s) 1153
Bsh1236I CGCG 2 cut(s) 637, 1668
BshFI GGCC 5 cut(s) 237, 392, 789, 889, 1659
BshVI ATCGAT 1 cut(s) 42
BsiHKCI CYCGRG 1 cut(s) 1147
BsiSI CCGG 3 cut(s) 294, 916, 1490
BslFI GGGAC 1 cut(s) 116
BslI CCNNNNNNNGG 5 cut(s) 436, 796, 1100, 1643, 1655
BsmAI GTCTC 1 cut(s) 1467
BsmFI GGGAC 1 cut(s) 116
BsmI GAATGC 1 cut(s) 511
BsnI GGCC 5 cut(s) 237, 392, 789, 889, 1659
BsoBI CYCGRG 1 cut(s) 1147
Bsp119I TTCGAA 1 cut(s) 1511
Bsp1286I GDGCHC 1 cut(s) 1497
Bsp13I TCCGGA 2 cut(s) 915, 1489
Bsp143I GATC 9 cut(s) 43, 204, 271, 553, 810, 1095, 1207, 1277, 1430
Bsp68I TCGCGA 1 cut(s) 1668
BspACI CCGC 3 cut(s) 1019, 1175, 1551
BspANI GGCC 5 cut(s) 237, 392, 789, 889, 1659
BspCNI CTCAG 1 cut(s) 1107
BspDI ATCGAT 1 cut(s) 42
BspEI TCCGGA 2 cut(s) 915, 1489
BspFNI CGCG 2 cut(s) 637, 1668
BspHI TCATGA 1 cut(s) 598
BspLI GGNNCC 1 cut(s) 1494
BspMI ACCTGC 1 cut(s) 1422
BspPI GGATC 3 cut(s) 199, 561, 1285
BspT104I TTCGAA 1 cut(s) 1511
BspTI CTTAAG 1 cut(s) 1211
BsrBI CCGCTC 1 cut(s) 1019
BsrDI GCAATG 1 cut(s) 663
BsrI ACTGG 6 cut(s) 79, 431, 723, 839, 1060, 1368
BssECI CCNNGG 3 cut(s) 393, 499, 869
BssMI GATC 9 cut(s) 43, 204, 271, 553, 810, 1095, 1207, 1277, 1430
BssNAI GTATAC 1 cut(s) 752
BssSI CACGAG 1 cut(s) 1014
BssT1I CCWWGG 3 cut(s) 393, 499, 869
Bst1107I GTATAC 1 cut(s) 752
Bst2BI CACGAG 1 cut(s) 1014
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 3 cut(s) 360, 610, 929
Bst6I CTCTTC 1 cut(s) 1734
BstAFI CTTAAG 1 cut(s) 1211
BstBI TTCGAA 1 cut(s) 1511
BstC8I GCNNGC 3 cut(s) 111, 561, 1657
BstDEI CTNAG 2 cut(s) 524, 1115
BstF5I GGATG 8 cut(s) 75, 157, 1133, 1336, 1372, 1393, 1676, 1767
BstFNI CGCG 2 cut(s) 637, 1668
BstH2I RGCGCY 1 cut(s) 1234
BstHHI GCGC 1 cut(s) 1233
BstKTI GATC 9 cut(s) 46, 207, 274, 556, 813, 1098, 1210, 1280, 1433
BstMAI GTCTC 1 cut(s) 1467
BstMBI GATC 9 cut(s) 43, 204, 271, 553, 810, 1095, 1207, 1277, 1430
BstMWI GCNNNNNNNGC 8 cut(s) 218, 227, 398, 569, 653, 1183, 1228, 1665
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 4 cut(s) 99, 113, 563, 1190
BstPAI GACNNNNGTC 1 cut(s) 253
BstSCI CCNGG 1 cut(s) 168
BstSFI CTRYAG 2 cut(s) 925, 1349
BstUI CGCG 2 cut(s) 637, 1668
BstV1I GCAGC 5 cut(s) 120, 142, 332, 427, 925
BstV2I GAAGAC 1 cut(s) 1362
BstX2I RGATCY 3 cut(s) 553, 1207, 1277
BstXI CCANNNNNNTGG 1 cut(s) 415
BstYI RGATCY 3 cut(s) 553, 1207, 1277
BstZ17I GTATAC 1 cut(s) 752
Bsu15I ATCGAT 1 cut(s) 42
BsuI GTATCC 1 cut(s) 1608
BsuRI GGCC 5 cut(s) 237, 392, 789, 889, 1659
BsuTUI ATCGAT 1 cut(s) 42
BtsCI GGATG 8 cut(s) 75, 157, 1133, 1336, 1372, 1393, 1676, 1767
BtsIMutI CAGTG 1 cut(s) 716
BtuMI TCGCGA 1 cut(s) 1668
BveI ACCTGC 1 cut(s) 1422
Cac8I GCNNGC 3 cut(s) 111, 561, 1657
CaiI CAGNNNCTG 1 cut(s) 136
CciI TCATGA 1 cut(s) 598
CfoI GCGC 1 cut(s) 1233
Cfr13I GGNCC 3 cut(s) 291, 1646, 1658
ClaI ATCGAT 1 cut(s) 42
CseI GACGC 2 cut(s) 201, 643
Csp6I GTAC 3 cut(s) 366, 847, 1152
CviAII CATG 9 cut(s) 96, 110, 560, 599, 709, 850, 934, 1187, 1192
CviQI GTAC 3 cut(s) 366, 847, 1152
DdeI CTNAG 2 cut(s) 524, 1115
DpnI GATC 9 cut(s) 45, 206, 273, 555, 812, 1097, 1209, 1279, 1432
DpnII GATC 9 cut(s) 43, 204, 271, 553, 810, 1095, 1207, 1277, 1430
Eam1104I CTCTTC 1 cut(s) 1734
EarI CTCTTC 1 cut(s) 1734
Eco130I CCWWGG 3 cut(s) 393, 499, 869
Eco147I AGGCCT 1 cut(s) 237
Eco24I GRGCYC 1 cut(s) 1497
Eco32I GATATC 1 cut(s) 901
Eco47I GGWCC 2 cut(s) 291, 1646
Eco57I CTGAAG 3 cut(s) 244, 669, 846
Eco88I CYCGRG 1 cut(s) 1147
EcoO109I RGGNCCY 1 cut(s) 1658
EcoRII CCWGG 1 cut(s) 168
EcoRV GATATC 1 cut(s) 901
EcoT14I CCWWGG 3 cut(s) 393, 499, 869
EcoT22I ATGCAT 1 cut(s) 1224
EcoT38I GRGCYC 1 cut(s) 1497
ErhI CCWWGG 3 cut(s) 393, 499, 869
FaeI CATG 9 cut(s) 99, 113, 563, 602, 712, 853, 937, 1190, 1195
FalI AAGNNNNNCTT 2 cut(s) 258, 290
FaqI GGGAC 1 cut(s) 116
FatI CATG 9 cut(s) 95, 109, 559, 598, 708, 849, 933, 1186, 1191
FauI CCCGC 1 cut(s) 1558
FauNDI CATATG 1 cut(s) 377
FblI GTMKAC 1 cut(s) 751
Fnu4HI GCNGC 5 cut(s) 131, 134, 321, 441, 939
FokI GGATG 7 cut(s) 82, 164, 1120, 1343, 1379, 1400, 1663
FriOI GRGCYC 1 cut(s) 1497
Fsp4HI GCNGC 5 cut(s) 131, 134, 321, 441, 939
FspBI CTAG 4 cut(s) 990, 1452, 1721, 1768
GlaI GCGC 1 cut(s) 1232
GluI GCNGC 5 cut(s) 131, 134, 321, 441, 939
HaeII RGCGCY 1 cut(s) 1234
HaeIII GGCC 5 cut(s) 237, 392, 789, 889, 1659
HapII CCGG 3 cut(s) 294, 916, 1490
HgaI GACGC 2 cut(s) 201, 643
HhaI GCGC 1 cut(s) 1233
Hin1II CATG 9 cut(s) 99, 113, 563, 602, 712, 853, 937, 1190, 1195
Hin6I GCGC 1 cut(s) 1231
HinP1I GCGC 1 cut(s) 1231
HincII GTYRAC 1 cut(s) 299
HindII GTYRAC 1 cut(s) 299
HinfI GANTC 8 cut(s) 249, 328, 419, 661, 907, 1007, 1670, 1689
HpaII CCGG 3 cut(s) 294, 916, 1490
HphI GGTGA 4 cut(s) 958, 1428, 1573, 1665
Hpy166II GTNNAC 4 cut(s) 299, 418, 752, 1144
Hpy188I TCNGA 7 cut(s) 673, 860, 1069, 1095, 1116, 1183, 1761
Hpy8I GTNNAC 4 cut(s) 299, 418, 752, 1144
Hpy99I CGWCG 1 cut(s) 1750
HpyAV CCTTC 8 cut(s) 118, 268, 500, 970, 1431, 1518, 1611, 1752
HpyCH4III ACNGT 3 cut(s) 360, 610, 929
HpyCH4IV ACGT 1 cut(s) 963
HpyF10VI GCNNNNNNNGC 8 cut(s) 218, 227, 398, 569, 653, 1183, 1228, 1665
HpyF3I CTNAG 2 cut(s) 524, 1115
HpySE526I ACGT 1 cut(s) 963
Hsp92II CATG 9 cut(s) 99, 113, 563, 602, 712, 853, 937, 1190, 1195
HspAI GCGC 1 cut(s) 1231
Kpn2I TCCGGA 2 cut(s) 915, 1489
Kzo9I GATC 9 cut(s) 43, 204, 271, 553, 810, 1095, 1207, 1277, 1430
LmnI GCTCC 3 cut(s) 127, 1195, 1492
Lsp1109I GCAGC 5 cut(s) 120, 142, 332, 427, 925
LweI GCATC 7 cut(s) 239, 361, 604, 643, 976, 1120, 1209
MaeI CTAG 4 cut(s) 990, 1452, 1721, 1768
MaeII ACGT 1 cut(s) 963
MaeIII GTNAC 6 cut(s) 213, 475, 929, 1118, 1289, 1709
MalI GATC 9 cut(s) 45, 206, 273, 555, 812, 1097, 1209, 1279, 1432
MbiI CCGCTC 1 cut(s) 1019
MboI GATC 9 cut(s) 43, 204, 271, 553, 810, 1095, 1207, 1277, 1430
MfeI CAATTG 1 cut(s) 883
MflI RGATCY 3 cut(s) 553, 1207, 1277
MhlI GDGCHC 1 cut(s) 1497
MluCI AATT 8 cut(s) 423, 805, 864, 883, 1001, 1087, 1308, 1473
MlyI GAGTC 4 cut(s) 243, 413, 655, 1664
MmeI TCCRAC 1 cut(s) 468
Mph1103I ATGCAT 1 cut(s) 1224
MroI TCCGGA 2 cut(s) 915, 1489
MroXI GAANNNNTTC 1 cut(s) 911
MseI TTAA 6 cut(s) 342, 843, 966, 1085, 1212, 1545
MslI CAYNNNNRTG 5 cut(s) 413, 449, 564, 1322, 1705
MspA1I CMGCKG 1 cut(s) 133
MspCI CTTAAG 1 cut(s) 1211
MspI CCGG 3 cut(s) 294, 916, 1490
MspR9I CCNGG 1 cut(s) 170
MunI CAATTG 1 cut(s) 883
Mva1269I GAATGC 1 cut(s) 511
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 2 cut(s) 637, 1668
MwoI GCNNNNNNNGC 8 cut(s) 218, 227, 398, 569, 653, 1183, 1228, 1665
NdeI CATATG 1 cut(s) 377
NdeII GATC 9 cut(s) 43, 204, 271, 553, 810, 1095, 1207, 1277, 1430
NlaIII CATG 9 cut(s) 99, 113, 563, 602, 712, 853, 937, 1190, 1195
NlaIV GGNNCC 1 cut(s) 1494
NmuCI GTSAC 3 cut(s) 213, 929, 1289
NruI TCGCGA 1 cut(s) 1668
NsiI ATGCAT 1 cut(s) 1224
NspI RCATGY 4 cut(s) 99, 113, 563, 1190
NspV TTCGAA 1 cut(s) 1511
PaeI GCATGC 2 cut(s) 113, 563
PaeR7I CTCGAG 1 cut(s) 1147
PagI TCATGA 1 cut(s) 598
PceI AGGCCT 1 cut(s) 237
PctI GAATGC 1 cut(s) 511
PdmI GAANNNNTTC 1 cut(s) 911
PfeI GAWTC 4 cut(s) 328, 907, 1007, 1689
PflMI CCANNNNNTGG 1 cut(s) 1643
PkrI GCNGC 5 cut(s) 132, 135, 322, 442, 940
PleI GAGTC 4 cut(s) 243, 413, 655, 1664
PpsI GAGTC 4 cut(s) 243, 413, 655, 1664
PshAI GACNNNNGTC 1 cut(s) 253
PshBI ATTAAT 1 cut(s) 1545
Psp6I CCWGG 1 cut(s) 168
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 1 cut(s) 1494
PspPI GGNCC 3 cut(s) 291, 1646, 1658
PspXI VCTCGAGB 1 cut(s) 1147
PstNI CAGNNNCTG 1 cut(s) 136
PsuI RGATCY 3 cut(s) 553, 1207, 1277
PvuII CAGCTG 1 cut(s) 133
RruI TCGCGA 1 cut(s) 1668
RsaI GTAC 3 cut(s) 367, 848, 1153
RsaNI GTAC 3 cut(s) 366, 847, 1152
RseI CAYNNNNRTG 5 cut(s) 413, 449, 564, 1322, 1705
SaqAI TTAA 6 cut(s) 342, 843, 966, 1085, 1212, 1545
SatI GCNGC 5 cut(s) 131, 134, 321, 441, 939
Sau3AI GATC 9 cut(s) 43, 204, 271, 553, 810, 1095, 1207, 1277, 1430
Sau96I GGNCC 3 cut(s) 291, 1646, 1658
ScaI AGTACT 1 cut(s) 1153
SchI GAGTC 4 cut(s) 243, 413, 655, 1664
ScrFI CCNGG 1 cut(s) 170
SduI GDGCHC 1 cut(s) 1497
SfaNI GCATC 7 cut(s) 239, 361, 604, 643, 976, 1120, 1209
SfcI CTRYAG 2 cut(s) 925, 1349
Sfr274I CTCGAG 1 cut(s) 1147
SfuI TTCGAA 1 cut(s) 1511
SinI GGWCC 2 cut(s) 291, 1646
SlaI CTCGAG 1 cut(s) 1147
SmiMI CAYNNNNRTG 5 cut(s) 413, 449, 564, 1322, 1705
SmlI CTYRAG 4 cut(s) 142, 275, 1147, 1211
SmoI CTYRAG 4 cut(s) 142, 275, 1147, 1211
SphI GCATGC 2 cut(s) 113, 563
Sse9I AATT 8 cut(s) 423, 805, 864, 883, 1001, 1087, 1308, 1473
SseBI AGGCCT 1 cut(s) 237
SsiI CCGC 3 cut(s) 1019, 1175, 1551
SspMI CTAG 4 cut(s) 990, 1452, 1721, 1768
StuI AGGCCT 1 cut(s) 237
StyD4I CCNGG 1 cut(s) 168
StyI CCWWGG 3 cut(s) 393, 499, 869
TaaI ACNGT 3 cut(s) 360, 610, 929
TaiI ACGT 1 cut(s) 966
TaqI TCGA 7 cut(s) 42, 174, 1148, 1511, 1687, 1738, 1745
TasI AATT 8 cut(s) 423, 805, 864, 883, 1001, 1087, 1308, 1473
TatI WGTACW 3 cut(s) 365, 846, 1151
TfiI GAWTC 4 cut(s) 328, 907, 1007, 1689
Tru1I TTAA 6 cut(s) 342, 843, 966, 1085, 1212, 1545
Tru9I TTAA 6 cut(s) 342, 843, 966, 1085, 1212, 1545
TscAI CASTG 1 cut(s) 723
TseFI GTSAC 3 cut(s) 213, 929, 1289
TseI GCWGC 5 cut(s) 130, 133, 320, 440, 938
Tsp45I GTSAC 3 cut(s) 213, 929, 1289
TspDTI ATGAA 7 cut(s) 137, 615, 725, 846, 1218, 1281, 1383
TspGWI ACGGA 3 cut(s) 150, 1523, 1722
TspRI CASTG 1 cut(s) 723
Van91I CCANNNNNTGG 1 cut(s) 1643
Vha464I CTTAAG 1 cut(s) 1211
VpaK11BI GGWCC 2 cut(s) 291, 1646
VspI ATTAAT 1 cut(s) 1545
XapI RAATTY 4 cut(s) 805, 864, 1087, 1473
XceI RCATGY 4 cut(s) 99, 113, 563, 1190
XhoI CTCGAG 1 cut(s) 1147
XmiI GTMKAC 1 cut(s) 751
XmnI GAANNNNTTC 1 cut(s) 911
XspI CTAG 4 cut(s) 990, 1452, 1721, 1768
ZrmI AGTACT 1 cut(s) 1153
Zsp2I ATGCAT 1 cut(s) 1224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.