FvH4_7g33450

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0007598
Physical Location & Seq
Forward (+)
23797197 .. 23799225
2029 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g33450.t2

Sequence Viewer

Length: 900 bp
ATGGCTGCTGACTCATCATCCCAATTCCAGATAGGTGCCCACGTGGAGGTCACGAGCCCAGTCCACAACATGCGCGGCTCGCTTTTCCCGGCCAGGATCGTCGCCGCCTCCGCCGACGGCACCAGATTGACCGTCGAGTACGACAAGGTCAAGACCAGGCTCTACCGCAAGGTCACCGGGCACAGCTCCGTGCGCGACGAGGTCCACGCGGCGCTCATCAGGCCGTCGCCGCCGCGGGAGGAGGGCTTCGCCGGATTCAAGGTCGGCGACGGCGTCGACGCGTTCTTGGCCGGCGGGTGGTGGGAGGGAGTGGTGACGGAGGAGCTGGACGACGCGAGATTCGAGGTGTTCTTTCAGGTTGTGAGGCAGAGATTTGAGTTTGAGGCGGAGGAGATGAGGATTCATAGGGAGTGGGTCAAAGGGGCTTGGGTTCCTCCTATTAGAGAAGAAGAGAAGGAAAAGAAGGCACAGCTGAAGGAAGAAATTCGAAAAGGGACAGAAGTTGAGGTTTCTAACGATGAGGATGGATTTCAAGGCTCATGGTTTGCAGCTACGGTTGTCCAAGTAGTGGCGAATGCCAAATTTCTTGTTCGGTATAAGAGCTTGAAGACAGATGATGGTAAAGAGTTCTTGACAGAAGAGATTGATGAAGAGCACTTGAGGCCTTGTCCCCCTGAAACTTATATGGTTGATTGTTACGGTCTGAATGACCTGGTTGATGCTTACTGGAATGAGGGATGGTGGGAAGGTAAGATATGTAAGGTGCTACATGGGGGAAGGTACAGGGTTTTCTTCGAGGGTACTGATGATAAAATGGTCTTTGACCACTCTGATTTAAGGCCACGCCAAGTTTGGATAGATGGAACTTGGGTTATGGCTTCTCAGGCACTCACGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

300

Amino Acids

34.23

Weight (kDa)

5.08

Isoelectric Point (pI)

30.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 12 - 78 5.9e-07 Agenet domain
Agenet PF05641 162 - 222 4.8e-21 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 35, 119
AccB7I CCANNNNNTGG 1 cut(s) 568
AccI GTMKAC 1 cut(s) 276
AccII CGCG 6 cut(s) 75, 195, 209, 235, 281, 335
AclWI GGATC 1 cut(s) 104
AcoI YGGCCR 2 cut(s) 90, 288
AcsI RAATTY 2 cut(s) 483, 581
AcuI CTGAAG 1 cut(s) 494
AcvI CACGTG 1 cut(s) 43
AcyI GRCGYC 1 cut(s) 273
AdeI CACNNNGTG 1 cut(s) 897
AfaI GTAC 3 cut(s) 140, 782, 802
AfiI CCNNNNNNNGG 3 cut(s) 46, 297, 568
AflIII ACRYGT 1 cut(s) 279
AgsI TTSAA 3 cut(s) 259, 533, 607
AjnI CCWGG 3 cut(s) 92, 155, 711
AluBI AGCT 5 cut(s) 186, 325, 472, 551, 603
AluI AGCT 5 cut(s) 186, 325, 472, 551, 603
Alw21I GWGCWC 1 cut(s) 657
AlwI GGATC 1 cut(s) 104
AoxI GGCC 5 cut(s) 90, 221, 288, 662, 839
ApeKI GCWGC 2 cut(s) 5, 548
ApoI RAATTY 2 cut(s) 483, 581
ArsI GACNNNNNNTTYG 2 cut(s) 323, 355
Asp700I GAANNNNTTC 1 cut(s) 483
AspLEI GCGC 3 cut(s) 75, 195, 214
AspS9I GGNCC 1 cut(s) 202
AsuC2I CCSGG 2 cut(s) 89, 178
AsuHPI GGTGA 2 cut(s) 166, 325
AsuII TTCGAA 1 cut(s) 487
AvaII GGWCC 1 cut(s) 202
BaeGI GKGCMC 2 cut(s) 40, 183
BanI GGYRCC 2 cut(s) 35, 119
BanII GRGCYC 1 cut(s) 59
BauI CACGAG 1 cut(s) 52
BbrPI CACGTG 1 cut(s) 43
BbsI GAAGAC 1 cut(s) 614
Bbv12I GWGCWC 1 cut(s) 657
BbvI GCAGC 1 cut(s) 560
BccI CCATC 4 cut(s) 518, 611, 732, 854
BceAI ACGGC 3 cut(s) 133, 208, 286
BciT130I CCWGG 3 cut(s) 94, 157, 713
BcnI CCSGG 2 cut(s) 89, 178
BfoI RGCGCY 1 cut(s) 215
BisI GCNGC 7 cut(s) 6, 76, 105, 210, 230, 233, 549
BlsI GCNGC 7 cut(s) 7, 77, 106, 211, 231, 234, 550
Bme1390I CCNGG 5 cut(s) 89, 94, 157, 178, 713
Bme18I GGWCC 1 cut(s) 202
BmgT120I GGNCC 1 cut(s) 202
BmiI GGNNCC 3 cut(s) 37, 121, 432
BmrFI CCNGG 5 cut(s) 89, 94, 157, 178, 713
BmrI ACTGGG 1 cut(s) 53
BmsI GCATC 1 cut(s) 709
BmuI ACTGGG 1 cut(s) 53
BpiI GAAGAC 1 cut(s) 614
Bpu14I TTCGAA 1 cut(s) 487
BpuEI CTTGAG 1 cut(s) 679
BpuMI CCSGG 2 cut(s) 89, 178
BsaAI YACGTR 1 cut(s) 43
BsaHI GRCGYC 1 cut(s) 273
BsaJI CCNNGG 1 cut(s) 233
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 297, 568
Bse118I RCCGGY 1 cut(s) 290
Bse1I ACTGG 2 cut(s) 59, 731
BseBI CCWGG 3 cut(s) 94, 157, 713
BseDI CCNNGG 1 cut(s) 233
BseGI GGATG 3 cut(s) 17, 529, 743
BseLI CCNNNNNNNGG 3 cut(s) 46, 297, 568
BseMII CTCAG 1 cut(s) 896
BseNI ACTGG 2 cut(s) 59, 731
BseRI GAGGAG 3 cut(s) 254, 335, 404
BseSI GKGCMC 2 cut(s) 40, 183
BseXI GCAGC 1 cut(s) 560
Bsh1236I CGCG 6 cut(s) 75, 195, 209, 235, 281, 335
BshFI GGCC 5 cut(s) 92, 223, 290, 664, 841
BshNI GGYRCC 2 cut(s) 35, 119
BsiHKAI GWGCWC 1 cut(s) 657
BsiSI CCGG 4 cut(s) 89, 177, 252, 291
BslFI GGGAC 2 cut(s) 508, 654
BslI CCNNNNNNNGG 3 cut(s) 46, 297, 568
BsmFI GGGAC 2 cut(s) 508, 654
BsmI GAATGC 1 cut(s) 580
BsnI GGCC 5 cut(s) 92, 223, 290, 664, 841
Bsp119I TTCGAA 1 cut(s) 487
Bsp1286I GDGCHC 4 cut(s) 40, 59, 183, 657
Bsp143I GATC 1 cut(s) 96
BspANI GGCC 5 cut(s) 92, 223, 290, 664, 841
BspCNI CTCAG 1 cut(s) 895
BspFNI CGCG 6 cut(s) 75, 195, 209, 235, 281, 335
BspLI GGNNCC 3 cut(s) 37, 121, 432
BspPI GGATC 1 cut(s) 104
BspQI GCTCTTC 1 cut(s) 645
BspT104I TTCGAA 1 cut(s) 487
BspT107I GGYRCC 2 cut(s) 35, 119
BsrFI RCCGGY 1 cut(s) 290
BsrI ACTGG 2 cut(s) 59, 731
BssAI RCCGGY 1 cut(s) 290
BssECI CCNNGG 1 cut(s) 233
BssMI GATC 1 cut(s) 96
BssNI GRCGYC 1 cut(s) 273
BssSI CACGAG 1 cut(s) 52
Bst2BI CACGAG 1 cut(s) 52
Bst2UI CCWGG 3 cut(s) 94, 157, 713
Bst4CI ACNGT 3 cut(s) 133, 556, 701
Bst6I CTCTTC 3 cut(s) 444, 633, 645
BstACI GRCGYC 1 cut(s) 273
BstBAI YACGTR 1 cut(s) 43
BstBI TTCGAA 1 cut(s) 487
BstC8I GCNNGC 2 cut(s) 80, 292
BstDEI CTNAG 1 cut(s) 882
BstDSI CCRYGG 1 cut(s) 233
BstEII GGTNACC 1 cut(s) 172
BstF5I GGATG 3 cut(s) 17, 529, 743
BstFNI CGCG 6 cut(s) 75, 195, 209, 235, 281, 335
BstH2I RGCGCY 1 cut(s) 215
BstHHI GCGC 3 cut(s) 75, 195, 214
BstKTI GATC 1 cut(s) 99
BstMBI GATC 1 cut(s) 96
BstMWI GCNNNNNNNGC 8 cut(s) 79, 110, 192, 220, 229, 287, 661, 884
BstNI CCWGG 3 cut(s) 94, 157, 713
BstNSI RCATGY 1 cut(s) 73
BstPI GGTNACC 1 cut(s) 172
BstSCI CCNGG 5 cut(s) 87, 92, 155, 176, 711
BstSLI GKGCMC 2 cut(s) 40, 183
BstUI CGCG 6 cut(s) 75, 195, 209, 235, 281, 335
BstV1I GCAGC 1 cut(s) 560
BstV2I GAAGAC 1 cut(s) 614
BsuRI GGCC 5 cut(s) 92, 223, 290, 664, 841
BtgI CCRYGG 1 cut(s) 233
BtsCI GGATG 3 cut(s) 17, 529, 743
Cac8I GCNNGC 2 cut(s) 80, 292
CfoI GCGC 3 cut(s) 75, 195, 214
Cfr10I RCCGGY 1 cut(s) 290
Cfr13I GGNCC 1 cut(s) 202
Cfr42I CCGCGG 1 cut(s) 236
CseI GACGC 3 cut(s) 262, 287, 341
CsiI ACCWGGT 1 cut(s) 711
Csp6I GTAC 3 cut(s) 139, 781, 801
CviAII CATG 3 cut(s) 70, 540, 770
CviQI GTAC 3 cut(s) 139, 781, 801
DdeI CTNAG 1 cut(s) 882
DpnI GATC 1 cut(s) 98
DpnII GATC 1 cut(s) 96
DraIII CACNNNGTG 1 cut(s) 897
EaeI YGGCCR 2 cut(s) 90, 288
Eam1104I CTCTTC 3 cut(s) 444, 633, 645
EarI CTCTTC 3 cut(s) 444, 633, 645
EciI GGCGGA 2 cut(s) 100, 401
Eco147I AGGCCT 1 cut(s) 664
Eco24I GRGCYC 1 cut(s) 59
Eco47I GGWCC 1 cut(s) 202
Eco57I CTGAAG 1 cut(s) 494
Eco72I CACGTG 1 cut(s) 43
Eco91I GGTNACC 1 cut(s) 172
EcoO65I GGTNACC 1 cut(s) 172
EcoRII CCWGG 3 cut(s) 92, 155, 711
EcoT38I GRGCYC 1 cut(s) 59
FaeI CATG 3 cut(s) 73, 543, 773
FaiI YATR 9 cut(s) 71, 405, 541, 597, 684, 686, 757, 771, 875
FaqI GGGAC 2 cut(s) 508, 654
FatI CATG 3 cut(s) 69, 539, 769
FauI CCCGC 2 cut(s) 228, 287
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 7 cut(s) 6, 76, 105, 210, 230, 233, 549
FokI GGATG 3 cut(s) 4, 536, 750
FriOI GRGCYC 1 cut(s) 59
Fsp4HI GCNGC 7 cut(s) 6, 76, 105, 210, 230, 233, 549
GlaI GCGC 3 cut(s) 74, 194, 213
GluI GCNGC 7 cut(s) 6, 76, 105, 210, 230, 233, 549
HaeII RGCGCY 1 cut(s) 215
HaeIII GGCC 5 cut(s) 92, 223, 290, 664, 841
HapII CCGG 4 cut(s) 89, 177, 252, 291
HgaI GACGC 3 cut(s) 262, 287, 341
HhaI GCGC 3 cut(s) 75, 195, 214
Hin1I GRCGYC 1 cut(s) 273
Hin1II CATG 3 cut(s) 73, 543, 773
Hin6I GCGC 3 cut(s) 73, 193, 212
HinP1I GCGC 3 cut(s) 73, 193, 212
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 4 cut(s) 11, 255, 339, 400
HpaII CCGG 4 cut(s) 89, 177, 252, 291
HphI GGTGA 2 cut(s) 166, 325
Hpy166II GTNNAC 3 cut(s) 64, 205, 277
Hpy188I TCNGA 2 cut(s) 705, 832
Hpy188III TCNNGA 4 cut(s) 28, 52, 151, 631
Hpy8I GTNNAC 3 cut(s) 64, 205, 277
Hpy99I CGWCG 9 cut(s) 104, 119, 137, 200, 229, 272, 278, 281, 335
HpyAV CCTTC 5 cut(s) 448, 457, 469, 740, 771
HpyCH4III ACNGT 3 cut(s) 133, 556, 701
HpyCH4IV ACGT 1 cut(s) 42
HpyCH4V TGCA 1 cut(s) 548
HpyF10VI GCNNNNNNNGC 8 cut(s) 79, 110, 192, 220, 229, 287, 661, 884
HpyF3I CTNAG 1 cut(s) 882
HpySE526I ACGT 1 cut(s) 42
Hsp92I GRCGYC 1 cut(s) 273
Hsp92II CATG 3 cut(s) 73, 543, 773
HspAI GCGC 3 cut(s) 73, 193, 212
KroI GCCGGC 1 cut(s) 290
KroNI GCCGGC 1 cut(s) 292
KspI CCGCGG 1 cut(s) 236
Kzo9I GATC 1 cut(s) 96
LguI GCTCTTC 1 cut(s) 645
LmnI GCTCC 2 cut(s) 191, 322
Lsp1109I GCAGC 1 cut(s) 560
LweI GCATC 1 cut(s) 709
MabI ACCWGGT 1 cut(s) 711
MaeII ACGT 1 cut(s) 42
MaeIII GTNAC 4 cut(s) 49, 172, 313, 695
MalI GATC 1 cut(s) 98
MboI GATC 1 cut(s) 96
MboII GAAGA 7 cut(s) 458, 461, 491, 619, 650, 662, 784
MhlI GDGCHC 4 cut(s) 40, 59, 183, 657
MluCI AATT 3 cut(s) 23, 483, 581
MluI ACGCGT 1 cut(s) 279
MlyI GAGTC 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 290
MroXI GAANNNNTTC 1 cut(s) 483
MseI TTAA 1 cut(s) 836
MspA1I CMGCKG 2 cut(s) 235, 472
MspI CCGG 4 cut(s) 89, 177, 252, 291
MspR9I CCNGG 5 cut(s) 89, 94, 157, 178, 713
Mva1269I GAATGC 1 cut(s) 580
MvaI CCWGG 3 cut(s) 94, 157, 713
MvnI CGCG 6 cut(s) 75, 195, 209, 235, 281, 335
MwoI GCNNNNNNNGC 8 cut(s) 79, 110, 192, 220, 229, 287, 661, 884
NaeI GCCGGC 1 cut(s) 292
NciI CCSGG 2 cut(s) 89, 178
NdeII GATC 1 cut(s) 96
NgoMIV GCCGGC 1 cut(s) 290
NlaIII CATG 3 cut(s) 73, 543, 773
NlaIV GGNNCC 3 cut(s) 37, 121, 432
NmuCI GTSAC 3 cut(s) 49, 172, 313
NspI RCATGY 1 cut(s) 73
NspV TTCGAA 1 cut(s) 487
PceI AGGCCT 1 cut(s) 664
PciSI GCTCTTC 1 cut(s) 645
PcsI WCGNNNNNNNCGW 2 cut(s) 270, 339
PctI GAATGC 1 cut(s) 580
PdiI GCCGGC 1 cut(s) 292
PdmI GAANNNNTTC 1 cut(s) 483
PfeI GAWTC 3 cut(s) 255, 339, 400
PflFI GACNNNGTC 3 cut(s) 146, 200, 272
PflMI CCANNNNNTGG 1 cut(s) 568
PkrI GCNGC 7 cut(s) 7, 77, 106, 211, 231, 234, 550
PleI GAGTC 1 cut(s) 5
PmaCI CACGTG 1 cut(s) 43
PmlI CACGTG 1 cut(s) 43
PpsI GAGTC 1 cut(s) 5
Ppu21I YACGTR 1 cut(s) 43
Psp6I CCWGG 3 cut(s) 92, 155, 711
PspCI CACGTG 1 cut(s) 43
PspEI GGTNACC 1 cut(s) 172
PspGI CCWGG 3 cut(s) 92, 155, 711
PspN4I GGNNCC 3 cut(s) 37, 121, 432
PspPI GGNCC 1 cut(s) 202
PsyI GACNNNGTC 3 cut(s) 146, 200, 272
PvuII CAGCTG 1 cut(s) 472
RsaI GTAC 3 cut(s) 140, 782, 802
RsaNI GTAC 3 cut(s) 139, 781, 801
SacII CCGCGG 1 cut(s) 236
SalI GTCGAC 1 cut(s) 275
SapI GCTCTTC 1 cut(s) 645
SaqAI TTAA 1 cut(s) 836
SatI GCNGC 7 cut(s) 6, 76, 105, 210, 230, 233, 549
Sau3AI GATC 1 cut(s) 96
Sau96I GGNCC 1 cut(s) 202
SchI GAGTC 1 cut(s) 5
ScrFI CCNGG 5 cut(s) 89, 94, 157, 178, 713
SduI GDGCHC 4 cut(s) 40, 59, 183, 657
SexAI ACCWGGT 1 cut(s) 711
SfaNI GCATC 1 cut(s) 709
Sfr303I CCGCGG 1 cut(s) 236
SfuI TTCGAA 1 cut(s) 487
SgrBI CCGCGG 1 cut(s) 236
SgrDI CGTCGACG 1 cut(s) 275
SinI GGWCC 1 cut(s) 202
SmlI CTYRAG 1 cut(s) 658
SmoI CTYRAG 1 cut(s) 658
Sse9I AATT 3 cut(s) 23, 483, 581
SseBI AGGCCT 1 cut(s) 664
StuI AGGCCT 1 cut(s) 664
StyD4I CCNGG 5 cut(s) 87, 92, 155, 176, 711
TaaI ACNGT 3 cut(s) 133, 556, 701
TaiI ACGT 1 cut(s) 45
TaqI TCGA 5 cut(s) 135, 276, 342, 487, 795
TasI AATT 3 cut(s) 23, 483, 581
TauI GCSGC 5 cut(s) 78, 107, 212, 232, 235
TfiI GAWTC 3 cut(s) 255, 339, 400
Tru1I TTAA 1 cut(s) 836
Tru9I TTAA 1 cut(s) 836
TseFI GTSAC 3 cut(s) 49, 172, 313
TseI GCWGC 2 cut(s) 5, 548
Tsp45I GTSAC 3 cut(s) 49, 172, 313
TspDTI ATGAA 2 cut(s) 392, 663
TspGWI ACGGA 2 cut(s) 178, 332
Tth111I GACNNNGTC 3 cut(s) 146, 200, 272
Van91I CCANNNNNTGG 1 cut(s) 568
VpaK11BI GGWCC 1 cut(s) 202
XapI RAATTY 2 cut(s) 483, 581
XceI RCATGY 1 cut(s) 73
XcmI CCANNNNNNNNNTGG 1 cut(s) 849
XmiI GTMKAC 1 cut(s) 276
XmnI GAANNNNTTC 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.