pycom01g24100

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0007598
Physical Location & Seq
Forward (+)
21154323 .. 21155879
1557 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g24100.1

Sequence Viewer

Length: 918 bp
ATGGCGGACGCCCACTTCGAGGTAGGCGACGAGGTGGAGGTCACATCCCCAATCCACAACATGCGCGGCACGACATTCCCGGCGAAAATCGTCGCCAAGTCGAGAACCAAATCGAAATTCAAAGTCGAATACAACAGAGCGAAGGCTCGGATCGATCGCAAGATCGGCGGCGGCCACAAGGGTGGCAATGCAGCCTTGAGGGAAGAGGTCGACGCTGCGCTCCTCCGTCCGTCGCCGCCGCGAGAGAGCGACTACCGGTTCAAATTAGGCGACGTGGTTGACGCCTTCTTTTGCGGCGGGTGGTGGGAGGGCGTCGTGGTCACTGACGTCTTGGTGGACAACACGTTCGGGGTTTACTTCCGGTTCGCCAAGGAGAAGTTTGAGTTCGAGCCGGAGGAGCTACGCCTTCACCGGGAGTGGGTCAATGGGTCCTGGATCCCACCTCTTCAACAAGGAGATGTCTTAGATGCCGAAGAACCAAAGATTCGGATGAAAGAGGAATTGATCGAAGGAACAGCGGTTGAGGTTTGCACTGACGAAGATGGATTTCAAGGTGCTTGGTTTGCTGCTAATATTGTCGAAGTAATGGAAAGGGACAAGTTTCTCATTCGATATAAGGCAATAAAAACAGATGATAACAGAGAGTTGTTGACGGAAGAGGTCGACGCAATGCACGTAAGGCCTCGTCCTCCAAAAGTTGTTGTGGCTGGAAGTTTCAGTCTGACGGAAGAGGTTGATGCTTTCTATAATGATGGCTGGTGGGAAGGTGTGATACGCAAGGTCCTTCACGGCCGAAGGTACAAAGTTTACTTCAAAGGTACAGACGACGAACTGCTGCTTCAGCACTCTGATTTGAGGCCACGCCAGGATTGGATAGATAGAACATGGGTTATGGCTTCTCAGGCATTGAAGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

306

Amino Acids

35.12

Weight (kDa)

5.53

Isoelectric Point (pI)

35.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 9 - 80 1.8e-07 Agenet domain
Agenet PF05641 171 - 231 2.4e-19 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 330
AccI GTMKAC 2 cut(s) 210, 663
AccII CGCG 2 cut(s) 66, 241
AciI CCGC 9 cut(s) 5, 66, 168, 171, 236, 239, 294, 297, 518
AclWI GGATC 3 cut(s) 158, 430, 443
AcoI YGGCCR 2 cut(s) 172, 790
AcsI RAATTY 1 cut(s) 116
AcuI CTGAAG 1 cut(s) 824
AcyI GRCGYC 4 cut(s) 9, 282, 312, 327
AfaI GTAC 2 cut(s) 800, 820
AfiI CCNNNNNNNGG 3 cut(s) 19, 412, 418
AflIII ACRYGT 1 cut(s) 342
AgeI ACCGGT 1 cut(s) 255
AgsI TTSAA 6 cut(s) 121, 262, 449, 551, 814, 910
AjiI CACGTC 1 cut(s) 274
AjnI CCWGG 2 cut(s) 431, 864
AjuI GAANNNNNNNTTGG 2 cut(s) 101, 133
AleI CACNNNNGTG 1 cut(s) 180
AloI GAACNNNNNNTCC 2 cut(s) 329, 361
AluBI AGCT 1 cut(s) 400
AluI AGCT 1 cut(s) 400
AlwI GGATC 3 cut(s) 158, 430, 443
AoxI GGCC 4 cut(s) 172, 680, 790, 857
ApeKI GCWGC 4 cut(s) 191, 215, 566, 835
ApoI RAATTY 1 cut(s) 116
ArsI GACNNNNNNTTYG 3 cut(s) 31, 329, 361
AsiGI ACCGGT 1 cut(s) 255
AspLEI GCGC 2 cut(s) 66, 220
AspS9I GGNCC 2 cut(s) 429, 781
AsuC2I CCSGG 2 cut(s) 80, 413
AsuHPI GGTGA 1 cut(s) 401
AvaII GGWCC 2 cut(s) 429, 781
BamHI GGATCC 1 cut(s) 435
BarI GAAGNNNNNNTAC 2 cut(s) 756, 788
BbvI GCAGC 4 cut(s) 202, 203, 553, 822
BccI CCATC 2 cut(s) 536, 746
BceAI ACGGC 1 cut(s) 805
BciT130I CCWGG 2 cut(s) 433, 866
BcnI CCSGG 2 cut(s) 80, 413
Bme1390I CCNGG 4 cut(s) 80, 413, 433, 866
Bme18I GGWCC 2 cut(s) 429, 781
BmgBI CACGTC 1 cut(s) 274
BmgT120I GGNCC 2 cut(s) 429, 781
BmiI GGNNCC 2 cut(s) 430, 437
BmrFI CCNGG 4 cut(s) 80, 413, 433, 866
BmsI GCATC 2 cut(s) 457, 727
BpuEI CTTGAG 1 cut(s) 217
BpuMI CCSGG 2 cut(s) 80, 413
Bsa29I ATCGAT 1 cut(s) 153
BsaAI YACGTR 1 cut(s) 676
BsaHI GRCGYC 4 cut(s) 9, 282, 312, 327
BsaJI CCNNGG 1 cut(s) 369
BsaWI WCCGGW 2 cut(s) 255, 360
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 412, 418
Bse118I RCCGGY 1 cut(s) 255
Bse3DI GCAATG 2 cut(s) 193, 675
BseBI CCWGG 2 cut(s) 433, 866
BseCI ATCGAT 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 369
BseGI GGATG 2 cut(s) 44, 495
BseLI CCNNNNNNNGG 3 cut(s) 19, 412, 418
BseMI GCAATG 2 cut(s) 193, 675
BseMII CTCAG 1 cut(s) 914
BseRI GAGGAG 2 cut(s) 212, 410
BseX3I CGGCCG 1 cut(s) 790
BseXI GCAGC 4 cut(s) 202, 203, 553, 822
Bsh1236I CGCG 2 cut(s) 66, 241
Bsh1285I CGRYCG 2 cut(s) 157, 793
BshFI GGCC 4 cut(s) 174, 682, 792, 859
BshTI ACCGGT 1 cut(s) 255
BshVI ATCGAT 1 cut(s) 153
BsiEI CGRYCG 2 cut(s) 157, 793
BsiSI CCGG 5 cut(s) 80, 256, 361, 392, 412
BslFI GGGAC 1 cut(s) 608
BslI CCNNNNNNNGG 3 cut(s) 19, 412, 418
BsmFI GGGAC 1 cut(s) 608
BsnI GGCC 4 cut(s) 174, 682, 792, 859
Bsp143I GATC 5 cut(s) 150, 154, 162, 435, 504
BspACI CCGC 9 cut(s) 5, 66, 168, 171, 236, 239, 294, 297, 518
BspANI GGCC 4 cut(s) 174, 682, 792, 859
BspCNI CTCAG 1 cut(s) 913
BspDI ATCGAT 1 cut(s) 153
BspFNI CGCG 2 cut(s) 66, 241
BspLI GGNNCC 2 cut(s) 430, 437
BspPI GGATC 3 cut(s) 158, 430, 443
BsrDI GCAATG 2 cut(s) 193, 675
BsrFI RCCGGY 1 cut(s) 255
BssAI RCCGGY 1 cut(s) 255
BssECI CCNNGG 1 cut(s) 369
BssMI GATC 5 cut(s) 150, 154, 162, 435, 504
BssNI GRCGYC 4 cut(s) 9, 282, 312, 327
BssT1I CCWWGG 1 cut(s) 369
Bst2UI CCWGG 2 cut(s) 433, 866
Bst6I CTCTTC 4 cut(s) 198, 450, 651, 723
BstACI GRCGYC 4 cut(s) 9, 282, 312, 327
BstBAI YACGTR 1 cut(s) 676
BstDEI CTNAG 2 cut(s) 463, 900
BstF5I GGATG 2 cut(s) 44, 495
BstFNI CGCG 2 cut(s) 66, 241
BstHHI GCGC 2 cut(s) 66, 220
BstKTI GATC 5 cut(s) 153, 157, 165, 438, 507
BstMBI GATC 5 cut(s) 150, 154, 162, 435, 504
BstMCI CGRYCG 2 cut(s) 157, 793
BstMWI GCNNNNNNNGC 6 cut(s) 165, 397, 563, 679, 841, 902
BstNI CCWGG 2 cut(s) 433, 866
BstNSI RCATGY 1 cut(s) 64
BstSCI CCNGG 4 cut(s) 78, 411, 431, 864
BstUI CGCG 2 cut(s) 66, 241
BstV1I GCAGC 4 cut(s) 202, 203, 553, 822
BstX2I RGATCY 1 cut(s) 435
BstXI CCANNNNNNTGG 1 cut(s) 182
BstYI RGATCY 1 cut(s) 435
BstZI CGGCCG 1 cut(s) 790
Bsu15I ATCGAT 1 cut(s) 153
BsuRI GGCC 4 cut(s) 174, 682, 792, 859
BsuTUI ATCGAT 1 cut(s) 153
BtrI CACGTC 1 cut(s) 274
BtsCI GGATG 2 cut(s) 44, 495
BtsIMutI CAGTG 2 cut(s) 321, 531
CfoI GCGC 2 cut(s) 66, 220
Cfr10I RCCGGY 1 cut(s) 255
Cfr13I GGNCC 2 cut(s) 429, 781
ClaI ATCGAT 1 cut(s) 153
CseI GACGC 5 cut(s) 17, 221, 290, 301, 674
Csp6I GTAC 2 cut(s) 799, 819
CspAI ACCGGT 1 cut(s) 255
CviAII CATG 2 cut(s) 61, 885
CviQI GTAC 2 cut(s) 799, 819
DdeI CTNAG 2 cut(s) 463, 900
DpnI GATC 5 cut(s) 152, 156, 164, 437, 506
DpnII GATC 5 cut(s) 150, 154, 162, 435, 504
EaeI YGGCCR 2 cut(s) 172, 790
EagI CGGCCG 1 cut(s) 790
Eam1104I CTCTTC 4 cut(s) 198, 450, 651, 723
EarI CTCTTC 4 cut(s) 198, 450, 651, 723
EciI GGCGGA 1 cut(s) 20
EclXI CGGCCG 1 cut(s) 790
Eco130I CCWWGG 1 cut(s) 369
Eco147I AGGCCT 1 cut(s) 682
Eco47I GGWCC 2 cut(s) 429, 781
Eco52I CGGCCG 1 cut(s) 790
Eco57I CTGAAG 1 cut(s) 824
EcoO109I RGGNCCY 2 cut(s) 429, 781
EcoRII CCWGG 2 cut(s) 431, 864
EcoT14I CCWWGG 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 2 cut(s) 64, 888
FaiI YATR 5 cut(s) 62, 615, 747, 886, 893
FaqI GGGAC 1 cut(s) 608
FatI CATG 2 cut(s) 60, 884
FauI CCCGC 1 cut(s) 290
FblI GTMKAC 2 cut(s) 210, 663
FokI GGATG 2 cut(s) 31, 502
GlaI GCGC 2 cut(s) 65, 219
HaeIII GGCC 4 cut(s) 174, 682, 792, 859
HapII CCGG 5 cut(s) 80, 256, 361, 392, 412
HgaI GACGC 5 cut(s) 17, 221, 290, 301, 674
HhaI GCGC 2 cut(s) 66, 220
Hin1I GRCGYC 4 cut(s) 9, 282, 312, 327
Hin1II CATG 2 cut(s) 64, 888
Hin6I GCGC 2 cut(s) 64, 218
HinP1I GCGC 2 cut(s) 64, 218
HincII GTYRAC 4 cut(s) 211, 280, 651, 664
HindII GTYRAC 4 cut(s) 211, 280, 651, 664
HinfI GANTC 1 cut(s) 484
HpaII CCGG 5 cut(s) 80, 256, 361, 392, 412
HphI GGTGA 1 cut(s) 401
Hpy166II GTNNAC 7 cut(s) 211, 280, 337, 355, 651, 664, 808
Hpy188I TCNGA 4 cut(s) 150, 489, 723, 850
Hpy188III TCNNGA 1 cut(s) 102
Hpy8I GTNNAC 7 cut(s) 211, 280, 337, 355, 651, 664, 808
Hpy99I CGWCG 8 cut(s) 32, 95, 215, 235, 275, 317, 668, 830
HpyAV CCTTC 7 cut(s) 136, 295, 416, 503, 758, 789, 794
HpyCH4IV ACGT 4 cut(s) 273, 327, 344, 675
HpyCH4V TGCA 3 cut(s) 191, 531, 673
HpyF10VI GCNNNNNNNGC 6 cut(s) 165, 397, 563, 679, 841, 902
HpyF3I CTNAG 2 cut(s) 463, 900
HpySE526I ACGT 4 cut(s) 273, 327, 344, 675
Hsp92I GRCGYC 4 cut(s) 9, 282, 312, 327
Hsp92II CATG 2 cut(s) 64, 888
HspAI GCGC 2 cut(s) 64, 218
Kzo9I GATC 5 cut(s) 150, 154, 162, 435, 504
LmnI GCTCC 2 cut(s) 225, 397
Lsp1109I GCAGC 4 cut(s) 202, 203, 553, 822
LweI GCATC 2 cut(s) 457, 727
MaeII ACGT 4 cut(s) 273, 327, 344, 675
MaeIII GTNAC 2 cut(s) 40, 319
MalI GATC 5 cut(s) 152, 156, 164, 437, 506
MboI GATC 5 cut(s) 150, 154, 162, 435, 504
MboII GAAGA 6 cut(s) 215, 437, 485, 551, 668, 740
MflI RGATCY 1 cut(s) 435
MluCI AATT 3 cut(s) 116, 263, 500
MslI CAYNNNNRTG 1 cut(s) 180
MspA1I CMGCKG 1 cut(s) 518
MspI CCGG 5 cut(s) 80, 256, 361, 392, 412
MspR9I CCNGG 4 cut(s) 80, 413, 433, 866
MvaI CCWGG 2 cut(s) 433, 866
MvnI CGCG 2 cut(s) 66, 241
MwoI GCNNNNNNNGC 6 cut(s) 165, 397, 563, 679, 841, 902
NciI CCSGG 2 cut(s) 80, 413
NdeII GATC 5 cut(s) 150, 154, 162, 435, 504
NlaIII CATG 2 cut(s) 64, 888
NlaIV GGNNCC 2 cut(s) 430, 437
NmuCI GTSAC 2 cut(s) 40, 319
NspI RCATGY 1 cut(s) 64
OliI CACNNNNGTG 1 cut(s) 180
PceI AGGCCT 1 cut(s) 682
PcsI WCGNNNNNNNCGW 3 cut(s) 15, 24, 672
PfeI GAWTC 1 cut(s) 484
PfoI TCCNGGA 1 cut(s) 431
PinAI ACCGGT 1 cut(s) 255
Ple19I CGATCG 1 cut(s) 157
Ppu21I YACGTR 1 cut(s) 676
PpuMI RGGWCCY 2 cut(s) 429, 781
Psp5II RGGWCCY 2 cut(s) 429, 781
Psp6I CCWGG 2 cut(s) 431, 864
PspGI CCWGG 2 cut(s) 431, 864
PspN4I GGNNCC 2 cut(s) 430, 437
PspPI GGNCC 2 cut(s) 429, 781
PspPPI RGGWCCY 2 cut(s) 429, 781
PsuI RGATCY 1 cut(s) 435
PvuI CGATCG 1 cut(s) 157
RsaI GTAC 2 cut(s) 800, 820
RsaNI GTAC 2 cut(s) 799, 819
RseI CAYNNNNRTG 1 cut(s) 180
SalI GTCGAC 2 cut(s) 209, 662
Sau3AI GATC 5 cut(s) 150, 154, 162, 435, 504
Sau96I GGNCC 2 cut(s) 429, 781
ScrFI CCNGG 4 cut(s) 80, 413, 433, 866
SfaNI GCATC 2 cut(s) 457, 727
SinI GGWCC 2 cut(s) 429, 781
SmiMI CAYNNNNRTG 1 cut(s) 180
SmlI CTYRAG 1 cut(s) 196
SmoI CTYRAG 1 cut(s) 196
Sse9I AATT 3 cut(s) 116, 263, 500
SseBI AGGCCT 1 cut(s) 682
SsiI CCGC 9 cut(s) 5, 66, 168, 171, 236, 239, 294, 297, 518
SspI AATATT 1 cut(s) 574
StuI AGGCCT 1 cut(s) 682
StyD4I CCNGG 4 cut(s) 78, 411, 431, 864
StyI CCWWGG 1 cut(s) 369
TaiI ACGT 4 cut(s) 276, 330, 347, 678
TasI AATT 3 cut(s) 116, 263, 500
TauI GCSGC 6 cut(s) 69, 171, 174, 238, 241, 297
TfiI GAWTC 1 cut(s) 484
TscAI CASTG 2 cut(s) 328, 538
TseFI GTSAC 2 cut(s) 40, 319
TseI GCWGC 4 cut(s) 191, 215, 566, 835
Tsp45I GTSAC 2 cut(s) 40, 319
TspDTI ATGAA 1 cut(s) 506
TspGWI ACGGA 4 cut(s) 215, 219, 668, 740
TspRI CASTG 2 cut(s) 328, 538
VpaK11BI GGWCC 2 cut(s) 429, 781
XapI RAATTY 1 cut(s) 116
XceI RCATGY 1 cut(s) 64
XcmI CCANNNNNNNNNTGG 1 cut(s) 867
XmiI GTMKAC 2 cut(s) 210, 663
ZraI GACGTC 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.