MD01G1232500.v1.1

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007598
Physical Location & Seq
Forward (+)
32099256 .. 32101257
2002 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1232500.v1.1.491

Sequence Viewer

Length: 921 bp
ATGGCGGACGCCCACTTCGAGGTAGGCGACGAGGTGGAGGTCACATCCCCAATCCACAACATGCGCGGCACCACATTCCCGGCGAAAATCGTCGCCAAGTCGAGAACCAAATCGAAATTCAAAGTCGAATACAACAGAGCGAAGGCTCGGATCGATCGCAAGATCGGCGCCGGCCACAAGGGTGGCGATGCAGCCTTGAGGGAAGAGGTCGACGCTGTGCTCCTCCGTCCGCCGCCGCCTCGAGAGAGTGACTACCGGTTCAAATTAGGCGACGTGGTCGACGCCTTCTTTTGCGGCGGGTGGTGGGAGGGCGTCGTGGTCACTGACGTTTTGGTGGACAACAACACGTTCGGGGTTTACTTCCGGTTCGCCAAGGAGAAGTTTGAGTTCGAGCCGGAGGAGCTACGCCTTCACCGGGAGTGGGTCAAGGGGTCCTGGATCCCACCTCTTCAACAAGGCGATGTCTCAGATGCCAAAGAACCAGAGATTCCGACGAAAGAGGAATTGATCGAAGGAACAGCGGTTGAGGTTTGCACTGACGAAGATGGATTTCAAGGTGCTTGGTTTGCTGCAAATATTGTCAAAGTAATGGAAAAGGACAAGTTTCTCGTTCGATACAAGGCAATAAAAACAGATGATAACAGAGAGTTGTTGACGGAAGAGGTCGACGCAAAGCACGTAAGGCCTCGTCCTCCAAAAGTTGTTGTGGCTGGAAGTTTCAGTCTGACGGAGGAGGTTGATGCTTTCTATAACGATGGCTGGTGGGAAGGTGTCATACGCAAGGTCCTTCACGGCCGAAGGTACAAAGTTTACTTCAAAGGTACAGACGACGAACTGCTGCTTCAGCACTCTGATTTGAGGCCACGCCAGGATTGGATAGATAGAACATGGGTTATGGCTTCTCAGGCATTGAAGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

307

Amino Acids

35.14

Weight (kDa)

5.73

Isoelectric Point (pI)

31.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 9 - 80 1.8e-07 Agenet domain
Agenet PF05641 172 - 232 7.9e-22 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 68, 167
AccI GTMKAC 3 cut(s) 210, 279, 666
AccII CGCG 1 cut(s) 66
AciI CCGC 8 cut(s) 5, 66, 230, 233, 236, 294, 297, 521
AclWI GGATC 3 cut(s) 158, 433, 446
AcoI YGGCCR 2 cut(s) 172, 793
AcsI RAATTY 1 cut(s) 116
AcuI CTGAAG 1 cut(s) 827
AcyI GRCGYC 4 cut(s) 9, 168, 282, 312
AfaI GTAC 2 cut(s) 803, 823
AfiI CCNNNNNNNGG 3 cut(s) 19, 415, 421
AflIII ACRYGT 1 cut(s) 345
AgeI ACCGGT 1 cut(s) 255
AgsI TTSAA 6 cut(s) 121, 262, 452, 554, 817, 913
AjiI CACGTC 1 cut(s) 274
AjnI CCWGG 2 cut(s) 434, 867
AjuI GAANNNNNNNTTGG 2 cut(s) 101, 133
AleI CACNNNNGTG 1 cut(s) 180
AluBI AGCT 1 cut(s) 403
AluI AGCT 1 cut(s) 403
Alw21I GWGCWC 1 cut(s) 222
Alw26I GTCTC 1 cut(s) 469
AlwI GGATC 3 cut(s) 158, 433, 446
Ama87I CYCGRG 1 cut(s) 240
AoxI GGCC 4 cut(s) 172, 683, 793, 860
ApeKI GCWGC 3 cut(s) 191, 569, 838
ApoI RAATTY 1 cut(s) 116
ArsI GACNNNNNNTTYG 1 cut(s) 31
AsiGI ACCGGT 1 cut(s) 255
AspLEI GCGC 2 cut(s) 66, 170
AspS9I GGNCC 2 cut(s) 432, 784
AsuC2I CCSGG 2 cut(s) 80, 416
AsuHPI GGTGA 1 cut(s) 404
AvaI CYCGRG 1 cut(s) 240
AvaII GGWCC 2 cut(s) 432, 784
BamHI GGATCC 1 cut(s) 438
BanI GGYRCC 2 cut(s) 68, 167
BarI GAAGNNNNNNTAC 2 cut(s) 759, 791
Bbv12I GWGCWC 1 cut(s) 222
BbvI GCAGC 3 cut(s) 203, 556, 825
BccI CCATC 2 cut(s) 539, 749
BceAI ACGGC 1 cut(s) 808
BciT130I CCWGG 2 cut(s) 436, 869
BcnI CCSGG 2 cut(s) 80, 416
BcoDI GTCTC 1 cut(s) 469
BfoI RGCGCY 1 cut(s) 171
BisI GCNGC 7 cut(s) 67, 192, 233, 236, 295, 570, 839
BlsI GCNGC 7 cut(s) 68, 193, 234, 237, 296, 571, 840
Bme1390I CCNGG 4 cut(s) 80, 416, 436, 869
Bme18I GGWCC 2 cut(s) 432, 784
BmeT110I CYCGRG 1 cut(s) 240
BmgBI CACGTC 1 cut(s) 274
BmgT120I GGNCC 2 cut(s) 432, 784
BmiI GGNNCC 4 cut(s) 70, 169, 433, 440
BmrFI CCNGG 4 cut(s) 80, 416, 436, 869
BmsI GCATC 3 cut(s) 178, 460, 730
BpuEI CTTGAG 1 cut(s) 217
BpuMI CCSGG 2 cut(s) 80, 416
Bsa29I ATCGAT 1 cut(s) 153
BsaAI YACGTR 1 cut(s) 679
BsaHI GRCGYC 4 cut(s) 9, 168, 282, 312
BsaJI CCNNGG 1 cut(s) 372
BsaWI WCCGGW 2 cut(s) 255, 363
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 415, 421
Bse118I RCCGGY 2 cut(s) 170, 255
BseBI CCWGG 2 cut(s) 436, 869
BseCI ATCGAT 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 372
BseGI GGATG 1 cut(s) 44
BseLI CCNNNNNNNGG 3 cut(s) 19, 415, 421
BseMII CTCAG 2 cut(s) 480, 917
BseRI GAGGAG 3 cut(s) 212, 413, 746
BseX3I CGGCCG 1 cut(s) 793
BseXI GCAGC 3 cut(s) 203, 556, 825
Bsh1236I CGCG 1 cut(s) 66
Bsh1285I CGRYCG 2 cut(s) 157, 796
BshFI GGCC 4 cut(s) 174, 685, 795, 862
BshNI GGYRCC 2 cut(s) 68, 167
BshTI ACCGGT 1 cut(s) 255
BshVI ATCGAT 1 cut(s) 153
BsiEI CGRYCG 2 cut(s) 157, 796
BsiHKAI GWGCWC 1 cut(s) 222
BsiHKCI CYCGRG 1 cut(s) 240
BsiSI CCGG 6 cut(s) 80, 171, 256, 364, 395, 415
BslI CCNNNNNNNGG 3 cut(s) 19, 415, 421
BsmAI GTCTC 1 cut(s) 469
BsnI GGCC 4 cut(s) 174, 685, 795, 862
BsoBI CYCGRG 1 cut(s) 240
Bsp1286I GDGCHC 1 cut(s) 222
Bsp143I GATC 5 cut(s) 150, 154, 162, 438, 507
BspACI CCGC 8 cut(s) 5, 66, 230, 233, 236, 294, 297, 521
BspANI GGCC 4 cut(s) 174, 685, 795, 862
BspCNI CTCAG 2 cut(s) 479, 916
BspDI ATCGAT 1 cut(s) 153
BspFNI CGCG 1 cut(s) 66
BspLI GGNNCC 4 cut(s) 70, 169, 433, 440
BspPI GGATC 3 cut(s) 158, 433, 446
BspT107I GGYRCC 2 cut(s) 68, 167
BsrFI RCCGGY 2 cut(s) 170, 255
BssAI RCCGGY 2 cut(s) 170, 255
BssECI CCNNGG 1 cut(s) 372
BssMI GATC 5 cut(s) 150, 154, 162, 438, 507
BssNI GRCGYC 4 cut(s) 9, 168, 282, 312
BssT1I CCWWGG 1 cut(s) 372
Bst2UI CCWGG 2 cut(s) 436, 869
Bst6I CTCTTC 3 cut(s) 198, 453, 654
BstACI GRCGYC 4 cut(s) 9, 168, 282, 312
BstBAI YACGTR 1 cut(s) 679
BstC8I GCNNGC 1 cut(s) 172
BstDEI CTNAG 2 cut(s) 466, 903
BstF5I GGATG 1 cut(s) 44
BstFNI CGCG 1 cut(s) 66
BstH2I RGCGCY 1 cut(s) 171
BstHHI GCGC 2 cut(s) 66, 170
BstKTI GATC 5 cut(s) 153, 157, 165, 441, 510
BstMAI GTCTC 1 cut(s) 469
BstMBI GATC 5 cut(s) 150, 154, 162, 438, 507
BstMCI CGRYCG 2 cut(s) 157, 796
BstMWI GCNNNNNNNGC 6 cut(s) 165, 400, 566, 682, 844, 905
BstNI CCWGG 2 cut(s) 436, 869
BstNSI RCATGY 1 cut(s) 64
BstSCI CCNGG 4 cut(s) 78, 414, 434, 867
BstUI CGCG 1 cut(s) 66
BstV1I GCAGC 3 cut(s) 203, 556, 825
BstX2I RGATCY 1 cut(s) 438
BstXI CCANNNNNNTGG 1 cut(s) 182
BstYI RGATCY 1 cut(s) 438
BstZI CGGCCG 1 cut(s) 793
Bsu15I ATCGAT 1 cut(s) 153
BsuRI GGCC 4 cut(s) 174, 685, 795, 862
BsuTUI ATCGAT 1 cut(s) 153
BtgZI GCGATG 2 cut(s) 201, 474
BtrI CACGTC 1 cut(s) 274
BtsCI GGATG 1 cut(s) 44
BtsIMutI CAGTG 2 cut(s) 321, 534
Cac8I GCNNGC 1 cut(s) 172
CfoI GCGC 2 cut(s) 66, 170
Cfr10I RCCGGY 2 cut(s) 170, 255
Cfr13I GGNCC 2 cut(s) 432, 784
ClaI ATCGAT 1 cut(s) 153
CseI GACGC 5 cut(s) 17, 221, 290, 301, 677
Csp6I GTAC 2 cut(s) 802, 822
CspAI ACCGGT 1 cut(s) 255
CviAII CATG 2 cut(s) 61, 888
CviQI GTAC 2 cut(s) 802, 822
DdeI CTNAG 2 cut(s) 466, 903
DinI GGCGCC 1 cut(s) 169
DpnI GATC 5 cut(s) 152, 156, 164, 440, 509
DpnII GATC 5 cut(s) 150, 154, 162, 438, 507
EaeI YGGCCR 2 cut(s) 172, 793
EagI CGGCCG 1 cut(s) 793
Eam1104I CTCTTC 3 cut(s) 198, 453, 654
EarI CTCTTC 3 cut(s) 198, 453, 654
EciI GGCGGA 2 cut(s) 20, 219
EclXI CGGCCG 1 cut(s) 793
Eco130I CCWWGG 1 cut(s) 372
Eco147I AGGCCT 1 cut(s) 685
Eco47I GGWCC 2 cut(s) 432, 784
Eco52I CGGCCG 1 cut(s) 793
Eco57I CTGAAG 1 cut(s) 827
Eco88I CYCGRG 1 cut(s) 240
EcoO109I RGGNCCY 2 cut(s) 432, 784
EcoRII CCWGG 2 cut(s) 434, 867
EcoT14I CCWWGG 1 cut(s) 372
EgeI GGCGCC 1 cut(s) 169
EheI GGCGCC 1 cut(s) 169
ErhI CCWWGG 1 cut(s) 372
FaeI CATG 2 cut(s) 64, 891
FaiI YATR 5 cut(s) 62, 750, 776, 889, 896
FatI CATG 2 cut(s) 60, 887
FauI CCCGC 1 cut(s) 290
FblI GTMKAC 3 cut(s) 210, 279, 666
Fnu4HI GCNGC 7 cut(s) 67, 192, 233, 236, 295, 570, 839
FokI GGATG 1 cut(s) 31
Fsp4HI GCNGC 7 cut(s) 67, 192, 233, 236, 295, 570, 839
GlaI GCGC 2 cut(s) 65, 169
GluI GCNGC 7 cut(s) 67, 192, 233, 236, 295, 570, 839
HaeII RGCGCY 1 cut(s) 171
HaeIII GGCC 4 cut(s) 174, 685, 795, 862
HapII CCGG 6 cut(s) 80, 171, 256, 364, 395, 415
HgaI GACGC 5 cut(s) 17, 221, 290, 301, 677
HhaI GCGC 2 cut(s) 66, 170
Hin1I GRCGYC 4 cut(s) 9, 168, 282, 312
Hin1II CATG 2 cut(s) 64, 891
Hin6I GCGC 2 cut(s) 64, 168
HinP1I GCGC 2 cut(s) 64, 168
HincII GTYRAC 4 cut(s) 211, 280, 654, 667
HindII GTYRAC 4 cut(s) 211, 280, 654, 667
HinfI GANTC 1 cut(s) 487
HpaII CCGG 6 cut(s) 80, 171, 256, 364, 395, 415
HphI GGTGA 1 cut(s) 404
Hpy166II GTNNAC 7 cut(s) 211, 280, 337, 358, 654, 667, 811
Hpy188I TCNGA 5 cut(s) 150, 469, 492, 726, 853
Hpy188III TCNNGA 2 cut(s) 102, 242
Hpy8I GTNNAC 7 cut(s) 211, 280, 337, 358, 654, 667, 811
Hpy99I CGWCG 9 cut(s) 32, 95, 215, 275, 284, 317, 496, 671, 833
HpyAV CCTTC 7 cut(s) 136, 295, 419, 506, 761, 792, 797
HpyCH4IV ACGT 4 cut(s) 273, 327, 347, 678
HpyCH4V TGCA 3 cut(s) 191, 534, 572
HpyF10VI GCNNNNNNNGC 6 cut(s) 165, 400, 566, 682, 844, 905
HpyF3I CTNAG 2 cut(s) 466, 903
HpySE526I ACGT 4 cut(s) 273, 327, 347, 678
Hsp92I GRCGYC 4 cut(s) 9, 168, 282, 312
Hsp92II CATG 2 cut(s) 64, 891
HspAI GCGC 2 cut(s) 64, 168
KasI GGCGCC 1 cut(s) 167
KroI GCCGGC 1 cut(s) 170
KroNI GCCGGC 1 cut(s) 172
Kzo9I GATC 5 cut(s) 150, 154, 162, 438, 507
LmnI GCTCC 2 cut(s) 225, 400
Lsp1109I GCAGC 3 cut(s) 203, 556, 825
LweI GCATC 3 cut(s) 178, 460, 730
MaeII ACGT 4 cut(s) 273, 327, 347, 678
MaeIII GTNAC 3 cut(s) 40, 248, 319
MalI GATC 5 cut(s) 152, 156, 164, 440, 509
MboI GATC 5 cut(s) 150, 154, 162, 438, 507
MboII GAAGA 4 cut(s) 215, 440, 554, 671
MflI RGATCY 1 cut(s) 438
MhlI GDGCHC 1 cut(s) 222
MluCI AATT 3 cut(s) 116, 263, 503
Mly113I GGCGCC 1 cut(s) 168
MmeI TCCRAC 1 cut(s) 515
MroNI GCCGGC 1 cut(s) 170
MslI CAYNNNNRTG 1 cut(s) 180
MspA1I CMGCKG 1 cut(s) 521
MspI CCGG 6 cut(s) 80, 171, 256, 364, 395, 415
MspR9I CCNGG 4 cut(s) 80, 416, 436, 869
MvaI CCWGG 2 cut(s) 436, 869
MvnI CGCG 1 cut(s) 66
MwoI GCNNNNNNNGC 6 cut(s) 165, 400, 566, 682, 844, 905
NaeI GCCGGC 1 cut(s) 172
NarI GGCGCC 1 cut(s) 168
NciI CCSGG 2 cut(s) 80, 416
NdeII GATC 5 cut(s) 150, 154, 162, 438, 507
NgoMIV GCCGGC 1 cut(s) 170
NlaIII CATG 2 cut(s) 64, 891
NlaIV GGNNCC 4 cut(s) 70, 169, 433, 440
NmuCI GTSAC 3 cut(s) 40, 248, 319
NspI RCATGY 1 cut(s) 64
OliI CACNNNNGTG 1 cut(s) 180
PaeR7I CTCGAG 1 cut(s) 240
PceI AGGCCT 1 cut(s) 685
PcsI WCGNNNNNNNCGW 3 cut(s) 15, 24, 675
PdiI GCCGGC 1 cut(s) 172
PfeI GAWTC 1 cut(s) 487
PflFI GACNNNGTC 1 cut(s) 275
PfoI TCCNGGA 1 cut(s) 434
PinAI ACCGGT 1 cut(s) 255
PkrI GCNGC 7 cut(s) 68, 193, 234, 237, 296, 571, 840
Ple19I CGATCG 1 cut(s) 157
PluTI GGCGCC 1 cut(s) 171
Ppu21I YACGTR 1 cut(s) 679
PpuMI RGGWCCY 2 cut(s) 432, 784
Psp5II RGGWCCY 2 cut(s) 432, 784
Psp6I CCWGG 2 cut(s) 434, 867
PspGI CCWGG 2 cut(s) 434, 867
PspN4I GGNNCC 4 cut(s) 70, 169, 433, 440
PspPI GGNCC 2 cut(s) 432, 784
PspPPI RGGWCCY 2 cut(s) 432, 784
PsuI RGATCY 1 cut(s) 438
PsyI GACNNNGTC 1 cut(s) 275
PvuI CGATCG 1 cut(s) 157
RsaI GTAC 2 cut(s) 803, 823
RsaNI GTAC 2 cut(s) 802, 822
RseI CAYNNNNRTG 1 cut(s) 180
SalI GTCGAC 3 cut(s) 209, 278, 665
SatI GCNGC 7 cut(s) 67, 192, 233, 236, 295, 570, 839
Sau3AI GATC 5 cut(s) 150, 154, 162, 438, 507
Sau96I GGNCC 2 cut(s) 432, 784
ScrFI CCNGG 4 cut(s) 80, 416, 436, 869
SduI GDGCHC 1 cut(s) 222
SfaNI GCATC 3 cut(s) 178, 460, 730
SfoI GGCGCC 1 cut(s) 169
Sfr274I CTCGAG 1 cut(s) 240
SinI GGWCC 2 cut(s) 432, 784
SlaI CTCGAG 1 cut(s) 240
SmiMI CAYNNNNRTG 1 cut(s) 180
SmlI CTYRAG 2 cut(s) 196, 240
SmoI CTYRAG 2 cut(s) 196, 240
Sse9I AATT 3 cut(s) 116, 263, 503
SseBI AGGCCT 1 cut(s) 685
SsiI CCGC 8 cut(s) 5, 66, 230, 233, 236, 294, 297, 521
SspDI GGCGCC 1 cut(s) 167
SspI AATATT 1 cut(s) 577
StuI AGGCCT 1 cut(s) 685
StyD4I CCNGG 4 cut(s) 78, 414, 434, 867
StyI CCWWGG 1 cut(s) 372
TaiI ACGT 4 cut(s) 276, 330, 350, 681
TasI AATT 3 cut(s) 116, 263, 503
TauI GCSGC 4 cut(s) 69, 235, 238, 297
TfiI GAWTC 1 cut(s) 487
TscAI CASTG 2 cut(s) 328, 541
TseFI GTSAC 3 cut(s) 40, 248, 319
TseI GCWGC 3 cut(s) 191, 569, 838
Tsp45I GTSAC 3 cut(s) 40, 248, 319
TspGWI ACGGA 3 cut(s) 215, 671, 743
TspRI CASTG 2 cut(s) 328, 541
Tth111I GACNNNGTC 1 cut(s) 275
VpaK11BI GGWCC 2 cut(s) 432, 784
XapI RAATTY 1 cut(s) 116
XceI RCATGY 1 cut(s) 64
XcmI CCANNNNNNNNNTGG 1 cut(s) 870
XhoI CTCGAG 1 cut(s) 240
XmiI GTMKAC 3 cut(s) 210, 279, 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.