MD00G1032200.v1.1

Belongs to the short-chain dehydrogenases reductases (SDR) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0016796
Physical Location & Seq
Forward (+)
5607861 .. 5611306
3446 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1032200.v1.1.491

Sequence Viewer

Length: 924 bp
ATGGGAAGCAATCAAGGAGATGGAGCAGAGACAGAGAGGTGTGCAGTAGTTAGCGGTGCAAACAAGGGTATTGGCTTGGAGGTGGTGAGGCAGCTTGCATCTCAAGGGGTGCTGGTGGTGTTGACGGCCAGGGATGAGAAGAGGGGAAGAGAAGCAACATCAAAGCTCCACAAGTTGGGTCTGTCAAATGTTGTCTTCCATCAACTTGATGTCTTGAATCCAGTTAGCATCCACTCCCTGGCCAAGTTCATACACGCCAGATTTGGCAGGCTTGATATCTTGGTTAATAATGCTGGAGCTACTGGAGCACTGTTGGATGAGAAAGTTTTAAAATCCCTAAACATTGACTCCAAAACTTGGCTTTCAGAGAAAGCGGTGAACCAAATTCAAGGTGCAATTAAATACACTTATGAGAAGGCTGAGGAATGCTTGAATACAAATTACTACGGGGTTAAAAGATTAACCGAGGCACTTCTCCCACTCTTGCAACTCTCCACTCTAGGAGCAAGAATTGTCAATGTCTCCTCCCTGAGGAGTGAACTCAAGAGGATACCAAGTGAAGAGATCCGAAATGAGCTCGGGGATGTAGAAGCACTGACAGAGGAGAAAGTGGATGCAGTTTTGACAAGATTTCTGCATGATTTTAAACAAAACGAACTCGAAGTGAATGGATGGACAATGATGTTACCTGCATATAGCATTTCCAAAGCCACCCTCAATGCCTACACTAGAATCCTTGCCAACAAGTACCCAAACATGTGCATCAACTGTGTGCATCCAGGCTTTGTTAATACAGACCTCAATTGCCATACAGGGACCATGACTGTCGAAGAAGGGGCTGCCGGTCCGGTCAAGTTGGCTCTTCTACCCCATGGAGGCCCTTCCGGCTGCTATTTCGATCAAACTGAAGTCGCAGATTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

308

Amino Acids

33.57

Weight (kDa)

6.26

Isoelectric Point (pI)

31.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 14 - 180 1.2e-23 short chain dehydrogenase
adh_short_C2 PF13561 19 - 111 8.7e-15 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016796)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27110
malus_domestica MD00G1032200.v1.1 MD11G1179400.v1.1
prunus_persica Prupe.6G151900_v2.0.a1
pyrus_communis pycom03g11580 pycom11g15490
rosa_chinensis RchiOBHm_Chr5g0049671
rosa_laevigata RLG00000034632
rosa_roxburghii Rroxscaffold_1G00031560
rosa_rugosa Rorug05G0250400
rosa_samantha Rh5BG337500 Rh5CG362200 Rh5DG349700
rosa_wichuraiana Rw5G030840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 697
AccB7I CCANNNNNTGG 3 cut(s) 175, 238, 357
AciI CCGC 2 cut(s) 54, 374
AclWI GGATC 1 cut(s) 559
AcoI YGGCCR 2 cut(s) 126, 240
AcsI RAATTY 1 cut(s) 384
AfaI GTAC 1 cut(s) 749
AfiI CCNNNNNNNGG 5 cut(s) 175, 238, 357, 531, 875
AflIII ACRYGT 1 cut(s) 756
AgsI TTSAA 3 cut(s) 217, 389, 433
AjnI CCWGG 3 cut(s) 128, 237, 778
AluBI AGCT 4 cut(s) 94, 166, 299, 577
AluI AGCT 4 cut(s) 94, 166, 299, 577
Alw21I GWGCWC 2 cut(s) 310, 579
Alw26I GTCTC 2 cut(s) 23, 526
AlwI GGATC 1 cut(s) 559
Ama87I CYCGRG 1 cut(s) 578
AoxI GGCC 3 cut(s) 126, 240, 877
ApeKI GCWGC 3 cut(s) 91, 839, 888
ApoI RAATTY 1 cut(s) 384
AspS9I GGNCC 3 cut(s) 816, 845, 878
AsuHPI GGTGA 2 cut(s) 97, 388
AvaI CYCGRG 1 cut(s) 578
AvaII GGWCC 2 cut(s) 816, 845
AxyI CCTNAGG 1 cut(s) 530
BalI TGGCCA 1 cut(s) 242
BanII GRGCYC 1 cut(s) 579
BbsI GAAGAC 1 cut(s) 187
Bbv12I GWGCWC 2 cut(s) 310, 579
BbvCI CCTCAGC 1 cut(s) 420
BbvI GCAGC 3 cut(s) 103, 826, 875
BccI CCATC 3 cut(s) 14, 207, 666
BceAI ACGGC 1 cut(s) 141
BciT130I CCWGG 3 cut(s) 130, 239, 780
BciVI GTATCC 1 cut(s) 543
BcoDI GTCTC 2 cut(s) 23, 526
BfaI CTAG 2 cut(s) 500, 729
BfuAI ACCTGC 1 cut(s) 697
BfuI GTATCC 1 cut(s) 543
BglI GCCNNNNNGGC 1 cut(s) 885
BisI GCNGC 3 cut(s) 92, 840, 889
BlsI GCNGC 3 cut(s) 93, 841, 890
Bme1390I CCNGG 3 cut(s) 130, 239, 780
Bme18I GGWCC 2 cut(s) 816, 845
BmeT110I CYCGRG 1 cut(s) 578
BmgT120I GGNCC 3 cut(s) 816, 845, 878
BmiI GGNNCC 1 cut(s) 817
BmrFI CCNGG 3 cut(s) 130, 239, 780
BmsI GCATC 5 cut(s) 107, 237, 604, 771, 784
BpiI GAAGAC 1 cut(s) 187
BpmI CTGGAG 2 cut(s) 315, 324
Bpu10I CCTNAGC 1 cut(s) 420
BpuEI CTTGAG 2 cut(s) 87, 527
BsaJI CCNNGG 4 cut(s) 129, 237, 465, 871
BsaWI WCCGGW 1 cut(s) 847
BsaXI ACNNNNNCTCC 2 cut(s) 332, 362
Bsc4I CCNNNNNNNGG 5 cut(s) 175, 238, 357, 531, 875
Bse118I RCCGGY 1 cut(s) 842
Bse1I ACTGG 2 cut(s) 221, 307
Bse21I CCTNAGG 1 cut(s) 530
BseBI CCWGG 3 cut(s) 130, 239, 780
BseDI CCNNGG 4 cut(s) 129, 237, 465, 871
BseGI GGATG 7 cut(s) 139, 228, 322, 589, 619, 677, 775
BseLI CCNNNNNNNGG 5 cut(s) 175, 238, 357, 531, 875
BseMII CTCAG 2 cut(s) 411, 521
BseNI ACTGG 2 cut(s) 221, 307
BseRI GAGGAG 3 cut(s) 514, 547, 617
BseXI GCAGC 3 cut(s) 103, 826, 875
BsgI GTGCAG 1 cut(s) 63
BshFI GGCC 3 cut(s) 128, 242, 879
BsiHKAI GWGCWC 2 cut(s) 310, 579
BsiHKCI CYCGRG 1 cut(s) 578
BsiSI CCGG 3 cut(s) 843, 848, 885
BslFI GGGAC 1 cut(s) 829
BslI CCNNNNNNNGG 5 cut(s) 175, 238, 357, 531, 875
BsmAI GTCTC 2 cut(s) 23, 526
BsmFI GGGAC 1 cut(s) 829
BsmI GAATGC 1 cut(s) 431
BsnI GGCC 3 cut(s) 128, 242, 879
BsoBI CYCGRG 1 cut(s) 578
Bsp1286I GDGCHC 2 cut(s) 310, 579
Bsp143I GATC 2 cut(s) 564, 898
Bsp19I CCATGG 1 cut(s) 871
BspACI CCGC 2 cut(s) 54, 374
BspANI GGCC 3 cut(s) 128, 242, 879
BspCNI CTCAG 2 cut(s) 412, 522
BspLI GGNNCC 1 cut(s) 817
BspMI ACCTGC 1 cut(s) 697
BspPI GGATC 1 cut(s) 559
BspQI GCTCTTC 1 cut(s) 867
BsrFI RCCGGY 1 cut(s) 842
BsrI ACTGG 2 cut(s) 221, 307
BssAI RCCGGY 1 cut(s) 842
BssECI CCNNGG 4 cut(s) 129, 237, 465, 871
BssMI GATC 2 cut(s) 564, 898
BssT1I CCWWGG 1 cut(s) 871
Bst2UI CCWGG 3 cut(s) 130, 239, 780
Bst4CI ACNGT 3 cut(s) 312, 770, 826
Bst6I CTCTTC 4 cut(s) 134, 142, 555, 867
BstC8I GCNNGC 2 cut(s) 96, 269
BstDEI CTNAG 2 cut(s) 420, 530
BstDSI CCRYGG 1 cut(s) 871
BstENI CCTNNNNNAGG 1 cut(s) 529
BstF5I GGATG 7 cut(s) 139, 228, 322, 589, 619, 677, 775
BstKTI GATC 2 cut(s) 567, 901
BstMAI GTCTC 2 cut(s) 23, 526
BstMBI GATC 2 cut(s) 564, 898
BstMWI GCNNNNNNNGC 2 cut(s) 305, 885
BstNI CCWGG 3 cut(s) 130, 239, 780
BstNSI RCATGY 1 cut(s) 760
BstSCI CCNGG 3 cut(s) 128, 237, 778
BstV1I GCAGC 3 cut(s) 103, 826, 875
BstV2I GAAGAC 1 cut(s) 187
BstX2I RGATCY 1 cut(s) 564
BstYI RGATCY 1 cut(s) 564
Bsu36I CCTNAGG 1 cut(s) 530
BsuI GTATCC 1 cut(s) 543
BsuRI GGCC 3 cut(s) 128, 242, 879
BtgI CCRYGG 1 cut(s) 871
BtsCI GGATG 7 cut(s) 139, 228, 322, 589, 619, 677, 775
BtsIMutI CAGTG 2 cut(s) 308, 593
BveI ACCTGC 1 cut(s) 697
Cac8I GCNNGC 2 cut(s) 96, 269
Cfr10I RCCGGY 1 cut(s) 842
Cfr13I GGNCC 3 cut(s) 816, 845, 878
CpoI CGGWCCG 1 cut(s) 845
Csp6I GTAC 1 cut(s) 748
CspI CGGWCCG 1 cut(s) 845
CviAII CATG 4 cut(s) 638, 757, 820, 872
CviQI GTAC 1 cut(s) 748
DdeI CTNAG 2 cut(s) 420, 530
DpnI GATC 2 cut(s) 566, 900
DpnII GATC 2 cut(s) 564, 898
DraI TTTAAA 2 cut(s) 330, 646
EaeI YGGCCR 2 cut(s) 126, 240
Eam1104I CTCTTC 4 cut(s) 134, 142, 555, 867
EarI CTCTTC 4 cut(s) 134, 142, 555, 867
Ecl136II GAGCTC 1 cut(s) 577
Eco130I CCWWGG 1 cut(s) 871
Eco24I GRGCYC 1 cut(s) 579
Eco32I GATATC 1 cut(s) 277
Eco47I GGWCC 2 cut(s) 816, 845
Eco53kI GAGCTC 1 cut(s) 577
Eco81I CCTNAGG 1 cut(s) 530
Eco88I CYCGRG 1 cut(s) 578
EcoICRI GAGCTC 1 cut(s) 577
EcoNI CCTNNNNNAGG 1 cut(s) 529
EcoO109I RGGNCCY 1 cut(s) 878
EcoRII CCWGG 3 cut(s) 128, 237, 778
EcoRV GATATC 1 cut(s) 277
EcoT14I CCWWGG 1 cut(s) 871
EcoT38I GRGCYC 1 cut(s) 579
ErhI CCWWGG 1 cut(s) 871
FaeI CATG 4 cut(s) 641, 760, 823, 875
FaiI YATR 9 cut(s) 251, 411, 639, 694, 696, 758, 810, 821, 873
FaqI GGGAC 1 cut(s) 829
FatI CATG 4 cut(s) 637, 756, 819, 871
Fnu4HI GCNGC 3 cut(s) 92, 840, 889
FokI GGATG 7 cut(s) 146, 215, 329, 596, 626, 684, 762
FriOI GRGCYC 1 cut(s) 579
Fsp4HI GCNGC 3 cut(s) 92, 840, 889
FspBI CTAG 2 cut(s) 500, 729
GluI GCNGC 3 cut(s) 92, 840, 889
GsuI CTGGAG 2 cut(s) 315, 324
HaeIII GGCC 3 cut(s) 128, 242, 879
HapII CCGG 3 cut(s) 843, 848, 885
Hin1II CATG 4 cut(s) 641, 760, 823, 875
HincII GTYRAC 1 cut(s) 123
HindII GTYRAC 1 cut(s) 123
HinfI GANTC 3 cut(s) 217, 347, 732
HpaII CCGG 3 cut(s) 843, 848, 885
HphI GGTGA 2 cut(s) 97, 388
Hpy166II GTNNAC 3 cut(s) 123, 379, 539
Hpy188I TCNGA 2 cut(s) 367, 569
Hpy188III TCNNGA 2 cut(s) 214, 544
Hpy8I GTNNAC 3 cut(s) 123, 379, 539
HpyAV CCTTC 3 cut(s) 409, 827, 891
HpyCH4III ACNGT 3 cut(s) 312, 770, 826
HpyF10VI GCNNNNNNNGC 2 cut(s) 305, 885
HpyF3I CTNAG 2 cut(s) 420, 530
Hsp92II CATG 4 cut(s) 641, 760, 823, 875
Kzo9I GATC 2 cut(s) 564, 898
LguI GCTCTTC 1 cut(s) 867
LmnI GCTCC 5 cut(s) 23, 171, 296, 305, 503
Lsp1109I GCAGC 3 cut(s) 103, 826, 875
LweI GCATC 5 cut(s) 107, 237, 604, 771, 784
MaeI CTAG 2 cut(s) 500, 729
MaeIII GTNAC 1 cut(s) 684
MalI GATC 2 cut(s) 566, 900
MboI GATC 2 cut(s) 564, 898
MboII GAAGA 6 cut(s) 151, 159, 187, 572, 842, 854
MfeI CAATTG 1 cut(s) 802
MflI RGATCY 1 cut(s) 564
MhlI GDGCHC 2 cut(s) 310, 579
MlsI TGGCCA 1 cut(s) 242
MluCI AATT 5 cut(s) 384, 396, 439, 510, 802
MluNI TGGCCA 1 cut(s) 242
MlyI GAGTC 1 cut(s) 341
MmeI TCCRAC 1 cut(s) 294
Mox20I TGGCCA 1 cut(s) 242
MscI TGGCCA 1 cut(s) 242
MseI TTAA 8 cut(s) 285, 329, 399, 453, 461, 645, 789, 922
Msp20I TGGCCA 1 cut(s) 242
MspI CCGG 3 cut(s) 843, 848, 885
MspR9I CCNGG 3 cut(s) 130, 239, 780
MunI CAATTG 1 cut(s) 802
Mva1269I GAATGC 1 cut(s) 431
MvaI CCWGG 3 cut(s) 130, 239, 780
MwoI GCNNNNNNNGC 2 cut(s) 305, 885
NcoI CCATGG 1 cut(s) 871
NdeII GATC 2 cut(s) 564, 898
NlaIII CATG 4 cut(s) 641, 760, 823, 875
NlaIV GGNNCC 1 cut(s) 817
NspI RCATGY 1 cut(s) 760
PciI ACATGT 1 cut(s) 756
PciSI GCTCTTC 1 cut(s) 867
PctI GAATGC 1 cut(s) 431
PfeI GAWTC 2 cut(s) 217, 732
PflMI CCANNNNNTGG 3 cut(s) 175, 238, 357
PkrI GCNGC 3 cut(s) 93, 841, 890
PleI GAGTC 1 cut(s) 341
PpsI GAGTC 1 cut(s) 341
PscI ACATGT 1 cut(s) 756
Psp124BI GAGCTC 1 cut(s) 579
Psp6I CCWGG 3 cut(s) 128, 237, 778
PspGI CCWGG 3 cut(s) 128, 237, 778
PspN4I GGNNCC 1 cut(s) 817
PspPI GGNCC 3 cut(s) 816, 845, 878
PsuI RGATCY 1 cut(s) 564
RsaI GTAC 1 cut(s) 749
RsaNI GTAC 1 cut(s) 748
Rsr2I CGGWCCG 1 cut(s) 845
RsrII CGGWCCG 1 cut(s) 845
SacI GAGCTC 1 cut(s) 579
SapI GCTCTTC 1 cut(s) 867
SaqAI TTAA 8 cut(s) 285, 329, 399, 453, 461, 645, 789, 922
SatI GCNGC 3 cut(s) 92, 840, 889
Sau3AI GATC 2 cut(s) 564, 898
Sau96I GGNCC 3 cut(s) 816, 845, 878
SchI GAGTC 1 cut(s) 341
ScrFI CCNGG 3 cut(s) 130, 239, 780
SduI GDGCHC 2 cut(s) 310, 579
SetI ASST 9 cut(s) 41, 84, 96, 168, 301, 394, 579, 691, 801
SfaNI GCATC 5 cut(s) 107, 237, 604, 771, 784
SinI GGWCC 2 cut(s) 816, 845
SmlI CTYRAG 2 cut(s) 102, 542
SmoI CTYRAG 2 cut(s) 102, 542
Sse9I AATT 5 cut(s) 384, 396, 439, 510, 802
SsiI CCGC 2 cut(s) 54, 374
SspMI CTAG 2 cut(s) 500, 729
SstI GAGCTC 1 cut(s) 579
StyD4I CCNGG 3 cut(s) 128, 237, 778
StyI CCWWGG 1 cut(s) 871
TaaI ACNGT 3 cut(s) 312, 770, 826
TaqI TCGA 3 cut(s) 660, 828, 897
TasI AATT 5 cut(s) 384, 396, 439, 510, 802
TfiI GAWTC 2 cut(s) 217, 732
Tru1I TTAA 8 cut(s) 285, 329, 399, 453, 461, 645, 789, 922
Tru9I TTAA 8 cut(s) 285, 329, 399, 453, 461, 645, 789, 922
TscAI CASTG 2 cut(s) 315, 600
TseI GCWGC 3 cut(s) 91, 839, 888
TspDTI ATGAA 1 cut(s) 238
TspRI CASTG 2 cut(s) 315, 600
Van91I CCANNNNNTGG 3 cut(s) 175, 238, 357
VpaK11BI GGWCC 2 cut(s) 816, 845
XagI CCTNNNNNAGG 1 cut(s) 529
XapI RAATTY 1 cut(s) 384
XceI RCATGY 1 cut(s) 760
XspI CTAG 2 cut(s) 500, 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.