MD00G1051600.v1.1

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0007484
Physical Location & Seq
Forward (+)
9693369 .. 9694241
873 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1051600.v1.1.491

Sequence Viewer

Length: 873 bp
ATGGAAGTGTATCACGAGACATGTCAGACCTTCACCTACGATGCCAAGCCCCTTGCCACACAAGTTCAGGACAGCTCCGATCGCCCTGAAAACCCTAGGGTTAGTCTTGAGATCGACTTATCACTCTTCGTAAACAAGAAGTACTACGTCAATATCTCTGAAGAATCCGAAGACGACGAAGTTGATAGGATTCCAATATTCCAAGACAAAGAACTGAAAGAATTACCTTCCAAAAGCCTAAGGATCACAGTACATGACGTGCGGTATTTAGACGATCATAATGCCTACAAAGAAAATAAGGATGGTATATCCGGCATGCTTAAAGAACATGAAATAATCAAGGGTGAGTCCATGCGCAATCTAGTGGTGGAAAGGATTCTGAACCAGGGTCGTAGGATTGGGGAGTCGGATTCAAACAAGGGTCCCAAAAAACTGCGGTTGAGGGTCGACATGAAAAGAGACCATATCTGGGACATGTGCGAAATGTGTTCGGATGAACGAGTCATTGCAAGGGACGCACAGCTTCAGAAGACGCTAAAGAGGGCTAGAGTGGTGGCTGATGACCATGACGATGAGGAGGACGAGGACGAGGACGAGGATGGTGATGAAGTTGGAGAAATAAAAAAAAGACGCATAGTGCATGAAGGCGAGGTTTGTTGCGTTTGCACGGAAGAGTTTGTTGCTGGATCAAAAGATGTGAAATACCTGCCTTGCTCGCATGTGTTTCATGGCAAATGCATAGGAGCATGGCTGAGAGAGAGAAAAGATAGCTGCCCTGTTTGCCGCTACTGCGTTGAGTTGCCTAATATTGAACACGGATCACAAGACAAAATTGAGAAGCCTATGTGCACTACTGATTTCAAAGATAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

291

Amino Acids

33.66

Weight (kDa)

5.03

Isoelectric Point (pI)

49.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 219 - 262 3.6e-12 Ring finger domain
zf-RING_11 PF17123 219 - 247 3.9e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 219 - 261 2.3e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 219 - 261 2.3e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 221 - 262 1.2e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 356
Acc36I ACCTGC 1 cut(s) 714
AccI GTMKAC 1 cut(s) 447
AciI CCGC 3 cut(s) 262, 436, 784
AclWI GGATC 3 cut(s) 251, 694, 826
AcuI CTGAAG 2 cut(s) 180, 509
AfaI GTAC 2 cut(s) 143, 252
AfiI CCNNNNNNNGG 1 cut(s) 469
AflIII ACRYGT 2 cut(s) 20, 474
AgsI TTSAA 3 cut(s) 414, 812, 862
AjiI CACGTC 1 cut(s) 259
AjnI CCWGG 1 cut(s) 384
AluBI AGCT 3 cut(s) 75, 523, 771
AluI AGCT 3 cut(s) 75, 523, 771
Alw21I GWGCWC 1 cut(s) 851
Alw26I GTCTC 2 cut(s) 11, 453
Alw44I GTGCAC 1 cut(s) 847
AlwI GGATC 3 cut(s) 251, 694, 826
ApaLI GTGCAC 1 cut(s) 847
ApeKI GCWGC 1 cut(s) 771
Asp700I GAANNNNTTC 1 cut(s) 375
AspA2I CCTAGG 1 cut(s) 95
AspLEI GCGC 1 cut(s) 357
AspS9I GGNCC 1 cut(s) 422
AsuHPI GGTGA 3 cut(s) 25, 356, 614
AvaII GGWCC 1 cut(s) 422
AvrII CCTAGG 1 cut(s) 95
AxyI CCTNAGG 1 cut(s) 239
BaeGI GKGCMC 1 cut(s) 851
BauI CACGAG 1 cut(s) 14
BbsI GAAGAC 2 cut(s) 177, 536
Bbv12I GWGCWC 1 cut(s) 851
BbvI GCAGC 1 cut(s) 758
BccI CCATC 2 cut(s) 296, 593
BcgI CGANNNNNNTGC 2 cut(s) 263, 297
BciT130I CCWGG 1 cut(s) 386
BcoDI GTCTC 2 cut(s) 11, 453
BfaI CTAG 3 cut(s) 96, 362, 546
BfuAI ACCTGC 1 cut(s) 714
BisI GCNGC 2 cut(s) 772, 784
BlnI CCTAGG 1 cut(s) 95
BlsI GCNGC 2 cut(s) 773, 785
BmcAI AGTACT 1 cut(s) 143
Bme1390I CCNGG 1 cut(s) 386
Bme18I GGWCC 1 cut(s) 422
BmgBI CACGTC 1 cut(s) 259
BmgT120I GGNCC 1 cut(s) 422
BmiI GGNNCC 2 cut(s) 423, 424
BmrFI CCNGG 1 cut(s) 386
BmsI GCATC 1 cut(s) 31
BpiI GAAGAC 2 cut(s) 177, 536
BpuEI CTTGAG 1 cut(s) 128
BsaI GGTCTC 1 cut(s) 453
BsaJI CCNNGG 2 cut(s) 95, 385
Bsc4I CCNNNNNNNGG 1 cut(s) 469
Bse21I CCTNAGG 1 cut(s) 239
Bse3DI GCAATG 1 cut(s) 504
BseBI CCWGG 1 cut(s) 386
BseDI CCNNGG 2 cut(s) 95, 385
BseGI GGATG 3 cut(s) 307, 499, 604
BseLI CCNNNNNNNGG 1 cut(s) 469
BseMI GCAATG 1 cut(s) 504
BseMII CTCAG 1 cut(s) 743
BseRI GAGGAG 1 cut(s) 590
BseSI GKGCMC 1 cut(s) 851
BseXI GCAGC 1 cut(s) 758
Bsh1285I CGRYCG 1 cut(s) 82
BsiEI CGRYCG 1 cut(s) 82
BsiHKAI GWGCWC 1 cut(s) 851
BsiSI CCGG 1 cut(s) 312
BslFI GGGAC 3 cut(s) 408, 485, 527
BslI CCNNNNNNNGG 1 cut(s) 469
BsmAI GTCTC 2 cut(s) 11, 453
BsmFI GGGAC 3 cut(s) 408, 485, 527
Bso31I GGTCTC 1 cut(s) 453
Bsp1286I GDGCHC 1 cut(s) 851
Bsp143I GATC 6 cut(s) 79, 111, 243, 274, 686, 818
BspACI CCGC 3 cut(s) 262, 436, 784
BspCNI CTCAG 1 cut(s) 744
BspLI GGNNCC 2 cut(s) 423, 424
BspMI ACCTGC 1 cut(s) 714
BspPI GGATC 3 cut(s) 251, 694, 826
BspTNI GGTCTC 1 cut(s) 453
BsrDI GCAATG 1 cut(s) 504
BssECI CCNNGG 2 cut(s) 95, 385
BssMI GATC 6 cut(s) 79, 111, 243, 274, 686, 818
BssSI CACGAG 1 cut(s) 14
BssT1I CCWWGG 1 cut(s) 95
Bst2BI CACGAG 1 cut(s) 14
Bst2UI CCWGG 1 cut(s) 386
Bst4CI ACNGT 1 cut(s) 250
Bst6I CTCTTC 2 cut(s) 131, 666
BstC8I GCNNGC 2 cut(s) 317, 716
BstDEI CTNAG 2 cut(s) 239, 752
BstF5I GGATG 3 cut(s) 307, 499, 604
BstHHI GCGC 1 cut(s) 357
BstKTI GATC 6 cut(s) 82, 114, 246, 277, 689, 821
BstMAI GTCTC 2 cut(s) 11, 453
BstMBI GATC 6 cut(s) 79, 111, 243, 274, 686, 818
BstMCI CGRYCG 1 cut(s) 82
BstMWI GCNNNNNNNGC 5 cut(s) 81, 515, 715, 780, 789
BstNI CCWGG 1 cut(s) 386
BstNSI RCATGY 4 cut(s) 24, 319, 478, 722
BstSCI CCNGG 1 cut(s) 384
BstSLI GKGCMC 1 cut(s) 851
BstV1I GCAGC 1 cut(s) 758
BstV2I GAAGAC 2 cut(s) 177, 536
Bsu36I CCTNAGG 1 cut(s) 239
BtrI CACGTC 1 cut(s) 259
BtsCI GGATG 3 cut(s) 307, 499, 604
BveI ACCTGC 1 cut(s) 714
Cac8I GCNNGC 2 cut(s) 317, 716
CfoI GCGC 1 cut(s) 357
Cfr13I GGNCC 1 cut(s) 422
CseI GACGC 3 cut(s) 524, 541, 639
Csp6I GTAC 2 cut(s) 142, 251
CviJI RGCY 9 cut(s) 49, 75, 237, 523, 545, 557, 751, 771, 841
CviKI_1 RGCY 9 cut(s) 49, 75, 237, 523, 545, 557, 751, 771, 841
CviQI GTAC 2 cut(s) 142, 251
DdeI CTNAG 2 cut(s) 239, 752
DpnI GATC 6 cut(s) 81, 113, 245, 276, 688, 820
DpnII GATC 6 cut(s) 79, 111, 243, 274, 686, 818
Eam1104I CTCTTC 2 cut(s) 131, 666
EarI CTCTTC 2 cut(s) 131, 666
Eco130I CCWWGG 1 cut(s) 95
Eco31I GGTCTC 1 cut(s) 453
Eco47I GGWCC 1 cut(s) 422
Eco57I CTGAAG 2 cut(s) 180, 509
Eco81I CCTNAGG 1 cut(s) 239
EcoO109I RGGNCCY 1 cut(s) 422
EcoRII CCWGG 1 cut(s) 384
EcoT14I CCWWGG 1 cut(s) 95
EcoT22I ATGCAT 1 cut(s) 740
ErhI CCWWGG 1 cut(s) 95
FaqI GGGAC 3 cut(s) 408, 485, 527
FblI GTMKAC 1 cut(s) 447
Fnu4HI GCNGC 2 cut(s) 772, 784
FokI GGATG 3 cut(s) 314, 506, 611
Fsp4HI GCNGC 2 cut(s) 772, 784
FspBI CTAG 3 cut(s) 96, 362, 546
FspI TGCGCA 1 cut(s) 356
GlaI GCGC 1 cut(s) 356
GluI GCNGC 2 cut(s) 772, 784
HapII CCGG 1 cut(s) 312
HgaI GACGC 3 cut(s) 524, 541, 639
HhaI GCGC 1 cut(s) 357
Hin6I GCGC 1 cut(s) 355
HinP1I GCGC 1 cut(s) 355
HincII GTYRAC 1 cut(s) 448
HindII GTYRAC 1 cut(s) 448
HinfI GANTC 7 cut(s) 164, 190, 347, 376, 404, 410, 501
HpaII CCGG 1 cut(s) 312
HphI GGTGA 3 cut(s) 25, 356, 614
Hpy166II GTNNAC 3 cut(s) 133, 448, 849
Hpy188I TCNGA 8 cut(s) 27, 79, 160, 169, 381, 409, 493, 528
Hpy188III TCNNGA 3 cut(s) 14, 68, 107
Hpy8I GTNNAC 3 cut(s) 133, 448, 849
Hpy99I CGWCG 1 cut(s) 179
HpyAV CCTTC 3 cut(s) 40, 237, 638
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4IV ACGT 2 cut(s) 147, 258
HpyCH4V TGCA 5 cut(s) 509, 640, 666, 738, 849
HpyF10VI GCNNNNNNNGC 5 cut(s) 81, 515, 715, 780, 789
HpyF3I CTNAG 2 cut(s) 239, 752
HpySE526I ACGT 2 cut(s) 147, 258
HspAI GCGC 1 cut(s) 355
KflI GGGWCCC 1 cut(s) 422
Kzo9I GATC 6 cut(s) 79, 111, 243, 274, 686, 818
LmnI GCTCC 2 cut(s) 80, 743
LpnPI CCDG 9 cut(s) 53, 99, 325, 371, 398, 454, 669, 719, 789
Lsp1109I GCAGC 1 cut(s) 758
LweI GCATC 1 cut(s) 31
MaeI CTAG 3 cut(s) 96, 362, 546
MaeII ACGT 2 cut(s) 147, 258
MalI GATC 6 cut(s) 81, 113, 245, 276, 688, 820
MboI GATC 6 cut(s) 79, 111, 243, 274, 686, 818
MboII GAAGA 5 cut(s) 118, 173, 182, 541, 683
MhlI GDGCHC 1 cut(s) 851
MluCI AATT 2 cut(s) 221, 831
MlyI GAGTC 3 cut(s) 356, 413, 510
MmeI TCCRAC 2 cut(s) 387, 592
MnlI CCTC 8 cut(s) 435, 534, 568, 571, 577, 583, 589, 643
Mph1103I ATGCAT 1 cut(s) 740
MroXI GAANNNNTTC 1 cut(s) 375
MseI TTAA 2 cut(s) 321, 871
MslI CAYNNNNRTG 1 cut(s) 570
MspI CCGG 1 cut(s) 312
MspR9I CCNGG 1 cut(s) 386
MvaI CCWGG 1 cut(s) 386
MwoI GCNNNNNNNGC 5 cut(s) 81, 515, 715, 780, 789
NdeII GATC 6 cut(s) 79, 111, 243, 274, 686, 818
NlaIV GGNNCC 2 cut(s) 423, 424
NsbI TGCGCA 1 cut(s) 356
NsiI ATGCAT 1 cut(s) 740
NspI RCATGY 4 cut(s) 24, 319, 478, 722
PaeI GCATGC 1 cut(s) 319
PciI ACATGT 2 cut(s) 20, 474
PdmI GAANNNNTTC 1 cut(s) 375
PfeI GAWTC 4 cut(s) 164, 190, 376, 410
PkrI GCNGC 2 cut(s) 773, 785
Ple19I CGATCG 1 cut(s) 82
PleI GAGTC 3 cut(s) 355, 412, 509
PpsI GAGTC 3 cut(s) 355, 412, 509
PpuMI RGGWCCY 1 cut(s) 422
PscI ACATGT 2 cut(s) 20, 474
Psp5II RGGWCCY 1 cut(s) 422
Psp6I CCWGG 1 cut(s) 384
PspGI CCWGG 1 cut(s) 384
PspN4I GGNNCC 2 cut(s) 423, 424
PspPI GGNCC 1 cut(s) 422
PspPPI RGGWCCY 1 cut(s) 422
PvuI CGATCG 1 cut(s) 82
RsaI GTAC 2 cut(s) 143, 252
RsaNI GTAC 2 cut(s) 142, 251
RseI CAYNNNNRTG 1 cut(s) 570
SalI GTCGAC 1 cut(s) 446
SaqAI TTAA 2 cut(s) 321, 871
SatI GCNGC 2 cut(s) 772, 784
Sau3AI GATC 6 cut(s) 79, 111, 243, 274, 686, 818
Sau96I GGNCC 1 cut(s) 422
ScaI AGTACT 1 cut(s) 143
SchI GAGTC 3 cut(s) 356, 413, 510
ScrFI CCNGG 1 cut(s) 386
SduI GDGCHC 1 cut(s) 851
SfaNI GCATC 1 cut(s) 31
SinI GGWCC 1 cut(s) 422
SmiMI CAYNNNNRTG 1 cut(s) 570
SmlI CTYRAG 1 cut(s) 107
SmoI CTYRAG 1 cut(s) 107
SphI GCATGC 1 cut(s) 319
Sse9I AATT 2 cut(s) 221, 831
SsiI CCGC 3 cut(s) 262, 436, 784
SspI AATATT 2 cut(s) 198, 808
SspMI CTAG 3 cut(s) 96, 362, 546
StyD4I CCNGG 1 cut(s) 384
StyI CCWWGG 1 cut(s) 95
TaaI ACNGT 1 cut(s) 250
TaiI ACGT 2 cut(s) 150, 261
TaqI TCGA 2 cut(s) 114, 447
TasI AATT 2 cut(s) 221, 831
TatI WGTACW 2 cut(s) 141, 250
TauI GCSGC 1 cut(s) 786
TfiI GAWTC 4 cut(s) 164, 190, 376, 410
Tru1I TTAA 2 cut(s) 321, 871
Tru9I TTAA 2 cut(s) 321, 871
TseI GCWGC 1 cut(s) 771
TspDTI ATGAA 6 cut(s) 345, 467, 510, 621, 657, 716
TspGWI ACGGA 2 cut(s) 683, 831
VneI GTGCAC 1 cut(s) 847
VpaK11BI GGWCC 1 cut(s) 422
XceI RCATGY 4 cut(s) 24, 319, 478, 722
XmaJI CCTAGG 1 cut(s) 95
XmiI GTMKAC 1 cut(s) 447
XmnI GAANNNNTTC 1 cut(s) 375
XspI CTAG 3 cut(s) 96, 362, 546
ZrmI AGTACT 1 cut(s) 143
Zsp2I ATGCAT 1 cut(s) 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.