MD12G1121000.v1.1

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007484
Physical Location & Seq
Forward (+)
19403592 .. 19404383
792 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1121000.v1.1.491

Sequence Viewer

Length: 792 bp
ATGGAAGTCGTCTATCACGAGATGAGCCAGAATTTTTACTGTCACGTTGATTCACTTGACACACAAGTACTACACCCCCTCGGCCATCACGAGCAATATTCTCCTTGGGTTCCACTTGAAATCTACTTCGAAATCTCCAGAAACTACAGCTTCTTCGACCGCGATCTCTCACAAGACGGAACCCTAACCCTGGTTCACCAATCTGAAGTGCAAGTCAGAAGTTACGAAGGCAAAAGAATTGATGCGTGCTGGTTTGGAACCCGAAACGAATACGTCAGACCCTATCTTTCTCAGGTGTTGTCCTTTCTTAAAATTCCTGAGGACATGCACAGTGATTTAGTGGAGACGATTATAGACAGGGGTATCCAAATCAGAAACTTGGATTCCAAGAAAGGTCCTAAAGTCCTGCGTATGAGGGTTGGTATAAAAAAATACCATGACTGGATTCGTTGTGAGTATTGTTCTAAAAAAAAACAACTCGAGAGGGATGCGCTGATCAAGAAGACGCTAAAGAGGGTTAGAGTGGTGACTGATGCTGATGATGTTAATGAGGTAGAGAGAGGCCGAGGAAGAAAAAGACGACGCGTAGTGCGTGAAAGTGAGGTTTGCTCTATTTGTTTGGAAGAGTTTGTTGTGGGATTAGAGGATTTGAGGTCCATGCCTTGCACTCATGTGTTTCATGAAAACTGCATATGGAGATGGTTCGAACGGAGTCACCTTAATTGCCCTATCTGCCGCTTCGAGATTTTTATTGAACGTGGATCACAAGACGAAGGCGACATTAGGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

264

Amino Acids

31.37

Weight (kDa)

6.83

Isoelectric Point (pI)

53.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 203 - 246 1.7e-11 Ring finger domain
zf-RING_11 PF17123 203 - 231 1.9e-09 RING-like zinc finger
zf-RING_UBOX PF13445 203 - 243 6.1e-09 RING-type zinc-finger
zf-C3HC4_2 PF13923 203 - 245 3.8e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 203 - 245 8.7e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 162, 585
AciI CCGC 2 cut(s) 160, 736
AclWI GGATC 1 cut(s) 769
AcoI YGGCCR 1 cut(s) 82
AcsI RAATTY 2 cut(s) 31, 312
AcuI CTGAAG 1 cut(s) 225
AfaI GTAC 1 cut(s) 69
AfiI CCNNNNNNNGG 1 cut(s) 190
AflIII ACRYGT 1 cut(s) 583
AgsI TTSAA 2 cut(s) 119, 755
AjnI CCWGG 1 cut(s) 189
AleI CACNNNNGTG 1 cut(s) 671
AluBI AGCT 1 cut(s) 150
AluI AGCT 1 cut(s) 150
Alw26I GTCTC 1 cut(s) 338
AlwI GGATC 1 cut(s) 769
Ama87I CYCGRG 1 cut(s) 479
AoxI GGCC 2 cut(s) 82, 562
ApoI RAATTY 2 cut(s) 31, 312
AspLEI GCGC 1 cut(s) 493
AspS9I GGNCC 2 cut(s) 395, 654
AsuHPI GGTGA 3 cut(s) 188, 538, 707
AsuII TTCGAA 2 cut(s) 129, 705
AvaI CYCGRG 1 cut(s) 479
AvaII GGWCC 2 cut(s) 395, 654
AxyI CCTNAGG 1 cut(s) 318
BaeI ACNNNNGTAYC 2 cut(s) 346, 379
BauI CACGAG 2 cut(s) 17, 89
BbsI GAAGAC 1 cut(s) 509
BccI CCATC 2 cut(s) 93, 693
BcgI CGANNNNNNTGC 2 cut(s) 470, 504
BciT130I CCWGG 1 cut(s) 191
BciVI GTATCC 1 cut(s) 374
BclI TGATCA 1 cut(s) 495
BcoDI GTCTC 1 cut(s) 338
BfmI CTRYAG 1 cut(s) 145
BfuI GTATCC 1 cut(s) 374
BisI GCNGC 1 cut(s) 736
BlsI GCNGC 1 cut(s) 737
BmcAI AGTACT 1 cut(s) 69
Bme1390I CCNGG 1 cut(s) 191
Bme18I GGWCC 2 cut(s) 395, 654
BmeT110I CYCGRG 1 cut(s) 479
BmgT120I GGNCC 2 cut(s) 395, 654
BmiI GGNNCC 3 cut(s) 111, 181, 259
BmrFI CCNGG 1 cut(s) 191
BmsI GCATC 3 cut(s) 232, 478, 523
BpiI GAAGAC 1 cut(s) 509
BplI GAGNNNNNCTC 2 cut(s) 593, 625
BpmI CTGGAG 1 cut(s) 121
Bpu14I TTCGAA 2 cut(s) 129, 705
BsaJI CCNNGG 4 cut(s) 79, 104, 189, 565
Bsc4I CCNNNNNNNGG 1 cut(s) 190
Bse1I ACTGG 1 cut(s) 446
Bse21I CCTNAGG 1 cut(s) 318
BseBI CCWGG 1 cut(s) 191
BseDI CCNNGG 4 cut(s) 79, 104, 189, 565
BseGI GGATG 1 cut(s) 493
BseLI CCNNNNNNNGG 1 cut(s) 190
BseMII CTCAG 2 cut(s) 305, 309
BseNI ACTGG 1 cut(s) 446
Bsh1236I CGCG 2 cut(s) 162, 585
Bsh1285I CGRYCG 1 cut(s) 160
BshFI GGCC 2 cut(s) 84, 564
BsiEI CGRYCG 1 cut(s) 160
BsiHKCI CYCGRG 1 cut(s) 479
BslI CCNNNNNNNGG 1 cut(s) 190
BsmAI GTCTC 1 cut(s) 338
BsmBI CGTCTC 1 cut(s) 338
BsnI GGCC 2 cut(s) 84, 564
BsoBI CYCGRG 1 cut(s) 479
Bsp119I TTCGAA 2 cut(s) 129, 705
Bsp143I GATC 3 cut(s) 163, 495, 761
BspACI CCGC 2 cut(s) 160, 736
BspANI GGCC 2 cut(s) 84, 564
BspCNI CTCAG 2 cut(s) 304, 310
BspFNI CGCG 2 cut(s) 162, 585
BspHI TCATGA 1 cut(s) 679
BspLI GGNNCC 3 cut(s) 111, 181, 259
BspPI GGATC 1 cut(s) 769
BspT104I TTCGAA 2 cut(s) 129, 705
BsrI ACTGG 1 cut(s) 446
BssECI CCNNGG 4 cut(s) 79, 104, 189, 565
BssMI GATC 3 cut(s) 163, 495, 761
BssSI CACGAG 2 cut(s) 17, 89
BssT1I CCWWGG 1 cut(s) 104
Bst2BI CACGAG 2 cut(s) 17, 89
Bst2UI CCWGG 1 cut(s) 191
Bst4CI ACNGT 2 cut(s) 41, 332
Bst6I CTCTTC 1 cut(s) 618
BstBI TTCGAA 2 cut(s) 129, 705
BstC8I GCNNGC 1 cut(s) 247
BstDEI CTNAG 2 cut(s) 291, 318
BstF5I GGATG 1 cut(s) 493
BstFNI CGCG 2 cut(s) 162, 585
BstHHI GCGC 1 cut(s) 493
BstKTI GATC 3 cut(s) 166, 498, 764
BstMAI GTCTC 1 cut(s) 338
BstMBI GATC 3 cut(s) 163, 495, 761
BstMCI CGRYCG 1 cut(s) 160
BstMWI GCNNNNNNNGC 1 cut(s) 732
BstNI CCWGG 1 cut(s) 191
BstNSI RCATGY 1 cut(s) 328
BstSCI CCNGG 1 cut(s) 189
BstSFI CTRYAG 1 cut(s) 145
BstUI CGCG 2 cut(s) 162, 585
BstV2I GAAGAC 1 cut(s) 509
Bsu36I CCTNAGG 1 cut(s) 318
BsuI GTATCC 1 cut(s) 374
BsuRI GGCC 2 cut(s) 84, 564
BtsCI GGATG 1 cut(s) 493
BtsIMutI CAGTG 1 cut(s) 337
Cac8I GCNNGC 1 cut(s) 247
CciI TCATGA 1 cut(s) 679
CfoI GCGC 1 cut(s) 493
Cfr13I GGNCC 2 cut(s) 395, 654
CseI GACGC 2 cut(s) 514, 591
Csp6I GTAC 1 cut(s) 68
CviAII CATG 5 cut(s) 325, 437, 658, 671, 680
CviJI RGCY 4 cut(s) 27, 84, 150, 564
CviKI_1 RGCY 4 cut(s) 27, 84, 150, 564
CviQI GTAC 1 cut(s) 68
DdeI CTNAG 2 cut(s) 291, 318
DpnI GATC 3 cut(s) 165, 497, 763
DpnII GATC 3 cut(s) 163, 495, 761
EaeI YGGCCR 1 cut(s) 82
Eam1104I CTCTTC 1 cut(s) 618
EarI CTCTTC 1 cut(s) 618
Eco130I CCWWGG 1 cut(s) 104
Eco47I GGWCC 2 cut(s) 395, 654
Eco57I CTGAAG 1 cut(s) 225
Eco81I CCTNAGG 1 cut(s) 318
Eco88I CYCGRG 1 cut(s) 479
EcoO109I RGGNCCY 1 cut(s) 395
EcoRII CCWGG 1 cut(s) 189
EcoT14I CCWWGG 1 cut(s) 104
ErhI CCWWGG 1 cut(s) 104
Esp3I CGTCTC 1 cut(s) 338
FaeI CATG 5 cut(s) 328, 440, 661, 674, 683
FatI CATG 5 cut(s) 324, 436, 657, 670, 679
FauNDI CATATG 1 cut(s) 692
FbaI TGATCA 1 cut(s) 495
Fnu4HI GCNGC 1 cut(s) 736
FokI GGATG 1 cut(s) 500
Fsp4HI GCNGC 1 cut(s) 736
GlaI GCGC 1 cut(s) 492
GluI GCNGC 1 cut(s) 736
GsuI CTGGAG 1 cut(s) 121
HaeIII GGCC 2 cut(s) 84, 564
HgaI GACGC 2 cut(s) 514, 591
HhaI GCGC 1 cut(s) 493
Hin1II CATG 5 cut(s) 328, 440, 661, 674, 683
Hin6I GCGC 1 cut(s) 491
HinP1I GCGC 1 cut(s) 491
HinfI GANTC 4 cut(s) 50, 383, 445, 712
HphI GGTGA 3 cut(s) 188, 538, 707
Hpy166II GTNNAC 1 cut(s) 196
Hpy188I TCNGA 4 cut(s) 205, 218, 278, 374
Hpy188III TCNNGA 8 cut(s) 17, 89, 138, 317, 481, 499, 680, 742
Hpy8I GTNNAC 1 cut(s) 196
Hpy99I CGWCG 1 cut(s) 585
HpyAV CCTTC 2 cut(s) 221, 767
HpyCH4III ACNGT 2 cut(s) 41, 332
HpyCH4IV ACGT 3 cut(s) 45, 273, 757
HpyCH4V TGCA 4 cut(s) 211, 328, 666, 690
HpyF10VI GCNNNNNNNGC 1 cut(s) 732
HpyF3I CTNAG 2 cut(s) 291, 318
HpySE526I ACGT 3 cut(s) 45, 273, 757
Hsp92II CATG 5 cut(s) 328, 440, 661, 674, 683
HspAI GCGC 1 cut(s) 491
Ksp22I TGATCA 1 cut(s) 495
Kzo9I GATC 3 cut(s) 163, 495, 761
LweI GCATC 3 cut(s) 232, 478, 523
MaeII ACGT 3 cut(s) 45, 273, 757
MaeIII GTNAC 4 cut(s) 41, 221, 526, 713
MalI GATC 3 cut(s) 165, 497, 763
MboI GATC 3 cut(s) 163, 495, 761
MboII GAAGA 4 cut(s) 145, 514, 582, 635
MluCI AATT 4 cut(s) 31, 237, 312, 721
MluI ACGCGT 1 cut(s) 583
MlyI GAGTC 1 cut(s) 721
MseI TTAA 4 cut(s) 309, 546, 720, 790
MslI CAYNNNNRTG 1 cut(s) 671
MspR9I CCNGG 1 cut(s) 191
MvaI CCWGG 1 cut(s) 191
MvnI CGCG 2 cut(s) 162, 585
MwoI GCNNNNNNNGC 1 cut(s) 732
NdeI CATATG 1 cut(s) 692
NdeII GATC 3 cut(s) 163, 495, 761
NlaIII CATG 5 cut(s) 328, 440, 661, 674, 683
NlaIV GGNNCC 3 cut(s) 111, 181, 259
NmeAIII GCCGAG 2 cut(s) 60, 590
NmuCI GTSAC 3 cut(s) 41, 526, 713
NspI RCATGY 1 cut(s) 328
NspV TTCGAA 2 cut(s) 129, 705
OliI CACNNNNGTG 1 cut(s) 671
PaeR7I CTCGAG 1 cut(s) 479
PagI TCATGA 1 cut(s) 679
PcsI WCGNNNNNNNCGW 3 cut(s) 15, 87, 589
PfeI GAWTC 3 cut(s) 50, 383, 445
PkrI GCNGC 1 cut(s) 737
PleI GAGTC 1 cut(s) 720
PpsI GAGTC 1 cut(s) 720
PpuMI RGGWCCY 1 cut(s) 395
Psp5II RGGWCCY 1 cut(s) 395
Psp6I CCWGG 1 cut(s) 189
PspGI CCWGG 1 cut(s) 189
PspN4I GGNNCC 3 cut(s) 111, 181, 259
PspPI GGNCC 2 cut(s) 395, 654
PspPPI RGGWCCY 1 cut(s) 395
RsaI GTAC 1 cut(s) 69
RsaNI GTAC 1 cut(s) 68
RseI CAYNNNNRTG 1 cut(s) 671
SaqAI TTAA 4 cut(s) 309, 546, 720, 790
SatI GCNGC 1 cut(s) 736
Sau3AI GATC 3 cut(s) 163, 495, 761
Sau96I GGNCC 2 cut(s) 395, 654
ScaI AGTACT 1 cut(s) 69
SchI GAGTC 1 cut(s) 721
ScrFI CCNGG 1 cut(s) 191
SfaNI GCATC 3 cut(s) 232, 478, 523
SfcI CTRYAG 1 cut(s) 145
Sfr274I CTCGAG 1 cut(s) 479
SfuI TTCGAA 2 cut(s) 129, 705
SinI GGWCC 2 cut(s) 395, 654
SlaI CTCGAG 1 cut(s) 479
SmiMI CAYNNNNRTG 1 cut(s) 671
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
Sse9I AATT 4 cut(s) 31, 237, 312, 721
SsiI CCGC 2 cut(s) 160, 736
SspI AATATT 1 cut(s) 98
StyD4I CCNGG 1 cut(s) 189
StyI CCWWGG 1 cut(s) 104
TaaI ACNGT 2 cut(s) 41, 332
TaiI ACGT 3 cut(s) 48, 276, 760
TaqI TCGA 5 cut(s) 129, 156, 480, 705, 741
TasI AATT 4 cut(s) 31, 237, 312, 721
TatI WGTACW 1 cut(s) 67
TauI GCSGC 1 cut(s) 738
TfiI GAWTC 3 cut(s) 50, 383, 445
Tru1I TTAA 4 cut(s) 309, 546, 720, 790
Tru9I TTAA 4 cut(s) 309, 546, 720, 790
TscAI CASTG 1 cut(s) 337
TseFI GTSAC 3 cut(s) 41, 526, 713
Tsp45I GTSAC 3 cut(s) 41, 526, 713
TspDTI ATGAA 2 cut(s) 668, 696
TspGWI ACGGA 2 cut(s) 192, 724
TspRI CASTG 1 cut(s) 337
VpaK11BI GGWCC 2 cut(s) 395, 654
XapI RAATTY 2 cut(s) 31, 312
XceI RCATGY 1 cut(s) 328
XhoI CTCGAG 1 cut(s) 479
ZrmI AGTACT 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.