MD05G1071800.v1.1

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0007484
Physical Location & Seq
Reverse (-)
15144765 .. 15145550
786 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1071800.v1.1.491

Sequence Viewer

Length: 786 bp
ATGGAAGTCGTCTATCACGAGATGAGCCAGAACATTTACTATCACGTTGATCCCCTTGACACACAAGTACTAGACCCCCTCGGCCATCATGAGAAATATTCTCCTTGCGTTCCACTGGAAATGTGCTTCGAAGTCTCCAGAACTACAGCTTCTTCAACCGCGCGATCTCTCGGAAGACGGACCCTGGTGCACCAATCAGAAGTTCAAGGGAGAAGTTACAAAGGCAAGATAATTGATGCGTGCTGGTTAGAAACCCGAAACGAATATGTCACACCTCATCTTTCTCAGGTGTTGTACTTTCTTAAAATCCACGAGGACAAGCACAGTGCTATGGTGGAGACGATTATAGACAGGGGTATCCTAATTAGAAACTTGGATTCCAAGAAAGGTCCTAAAGTCCTGAGCATGACGGTTGATATGAAAAGATACCATAACTGGATTCGCTGTGAAGATTGTTCGAAAGAAAAACAACTCAAGAGGGATGCGTTGATCAAGAAGACGCTGAAGAGGGTTAGAGTGGTGGCTTATGCCGATGATGTTAATGAGGAAGAGAGAGGTCGAGGGAGAAAAAGACGATGCATAGTGCGTGAAAGTGAGGTTTGCCCTATTTGTCTCGAAGAGTTCGTTGTTGGATTAGAGGATGTGAGGTCCATGCCTTGCACGCATGTGTTTCATGAAAACTGCATATGGAGATGGTTCGAACGGAGTCACCTTGATTGCCCTATTTGCCCCTTCGATATTTTTATTGAACCTGGATCACATGGCGAAGGCGACATCAGGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

262

Amino Acids

30.53

Weight (kDa)

6.89

Isoelectric Point (pI)

55.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 201 - 243 3.2e-11 Ring finger domain
zf-RING_11 PF17123 201 - 229 9.2e-09 RING-like zinc finger
zf-C3HC4_2 PF13923 201 - 243 1.1e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 201 - 241 1.3e-08 RING-type zinc-finger
zf-C3HC4 PF00097 201 - 243 6.7e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 161, 163
AciI CCGC 1 cut(s) 159
AclWI GGATC 2 cut(s) 44, 763
AcoI YGGCCR 1 cut(s) 82
AcuI CTGAAG 1 cut(s) 524
AfaI GTAC 2 cut(s) 69, 296
AgsI TTSAA 3 cut(s) 156, 206, 749
AjnI CCWGG 2 cut(s) 183, 751
AjuI GAANNNNNNNTTGG 2 cut(s) 186, 218
AleI CACNNNNGTG 1 cut(s) 665
AluBI AGCT 1 cut(s) 149
AluI AGCT 1 cut(s) 149
Alw21I GWGCWC 1 cut(s) 192
Alw26I GTCTC 3 cut(s) 139, 332, 617
Alw44I GTGCAC 1 cut(s) 188
AlwI GGATC 2 cut(s) 44, 763
AoxI GGCC 1 cut(s) 82
ApaLI GTGCAC 1 cut(s) 188
AspLEI GCGC 1 cut(s) 163
AspS9I GGNCC 3 cut(s) 180, 389, 648
AsuHPI GGTGA 1 cut(s) 701
AsuII TTCGAA 3 cut(s) 129, 458, 699
AvaII GGWCC 3 cut(s) 180, 389, 648
BaeGI GKGCMC 1 cut(s) 192
BaeI ACNNNNGTAYC 2 cut(s) 340, 373
BauI CACGAG 2 cut(s) 17, 311
BbsI GAAGAC 2 cut(s) 181, 503
Bbv12I GWGCWC 1 cut(s) 192
BccI CCATC 2 cut(s) 93, 687
BciT130I CCWGG 2 cut(s) 185, 753
BciVI GTATCC 1 cut(s) 368
BclI TGATCA 1 cut(s) 489
BcoDI GTCTC 3 cut(s) 139, 332, 617
BfaI CTAG 1 cut(s) 71
BfmI CTRYAG 1 cut(s) 144
BfuI GTATCC 1 cut(s) 368
BmcAI AGTACT 1 cut(s) 69
Bme1390I CCNGG 2 cut(s) 185, 753
Bme18I GGWCC 3 cut(s) 180, 389, 648
BmgT120I GGNCC 3 cut(s) 180, 389, 648
BmiI GGNNCC 1 cut(s) 182
BmrFI CCNGG 2 cut(s) 185, 753
BmsI GCATC 3 cut(s) 226, 472, 566
BpiI GAAGAC 2 cut(s) 181, 503
BpmI CTGGAG 1 cut(s) 121
Bpu10I CCTNAGC 1 cut(s) 401
Bpu14I TTCGAA 3 cut(s) 129, 458, 699
BpuEI CTTGAG 1 cut(s) 458
BsaJI CCNNGG 2 cut(s) 79, 183
Bse1I ACTGG 2 cut(s) 120, 440
BseBI CCWGG 2 cut(s) 185, 753
BseDI CCNNGG 2 cut(s) 79, 183
BseGI GGATG 2 cut(s) 487, 646
BseMII CTCAG 2 cut(s) 299, 392
BseNI ACTGG 2 cut(s) 120, 440
BseSI GKGCMC 1 cut(s) 192
Bsh1236I CGCG 2 cut(s) 161, 163
BshFI GGCC 1 cut(s) 84
BsiHKAI GWGCWC 1 cut(s) 192
BsmAI GTCTC 3 cut(s) 139, 332, 617
BsmBI CGTCTC 1 cut(s) 332
BsnI GGCC 1 cut(s) 84
Bsp119I TTCGAA 3 cut(s) 129, 458, 699
Bsp1286I GDGCHC 1 cut(s) 192
Bsp143I GATC 4 cut(s) 49, 164, 489, 755
BspACI CCGC 1 cut(s) 159
BspANI GGCC 1 cut(s) 84
BspCNI CTCAG 2 cut(s) 298, 393
BspFNI CGCG 2 cut(s) 161, 163
BspHI TCATGA 2 cut(s) 88, 673
BspLI GGNNCC 1 cut(s) 182
BspPI GGATC 2 cut(s) 44, 763
BspT104I TTCGAA 3 cut(s) 129, 458, 699
BsrI ACTGG 2 cut(s) 120, 440
BssECI CCNNGG 2 cut(s) 79, 183
BssMI GATC 4 cut(s) 49, 164, 489, 755
BssSI CACGAG 2 cut(s) 17, 311
Bst2BI CACGAG 2 cut(s) 17, 311
Bst2UI CCWGG 2 cut(s) 185, 753
Bst4CI ACNGT 2 cut(s) 326, 412
Bst6I CTCTTC 3 cut(s) 500, 543, 612
BstBI TTCGAA 3 cut(s) 129, 458, 699
BstC8I GCNNGC 2 cut(s) 241, 662
BstDEI CTNAG 2 cut(s) 285, 401
BstF5I GGATG 2 cut(s) 487, 646
BstFNI CGCG 2 cut(s) 161, 163
BstHHI GCGC 1 cut(s) 163
BstKTI GATC 4 cut(s) 52, 167, 492, 758
BstMAI GTCTC 3 cut(s) 139, 332, 617
BstMBI GATC 4 cut(s) 49, 164, 489, 755
BstMWI GCNNNNNNNGC 2 cut(s) 661, 726
BstNI CCWGG 2 cut(s) 185, 753
BstNSI RCATGY 1 cut(s) 668
BstSCI CCNGG 2 cut(s) 183, 751
BstSFI CTRYAG 1 cut(s) 144
BstSLI GKGCMC 1 cut(s) 192
BstUI CGCG 2 cut(s) 161, 163
BstV2I GAAGAC 2 cut(s) 181, 503
BsuI GTATCC 1 cut(s) 368
BsuRI GGCC 1 cut(s) 84
BtsCI GGATG 2 cut(s) 487, 646
BtsIMutI CAGTG 2 cut(s) 113, 331
Cac8I GCNNGC 2 cut(s) 241, 662
CciI TCATGA 2 cut(s) 88, 673
CfoI GCGC 1 cut(s) 163
Cfr13I GGNCC 3 cut(s) 180, 389, 648
CseI GACGC 1 cut(s) 508
Csp6I GTAC 2 cut(s) 68, 295
CviAII CATG 6 cut(s) 89, 406, 652, 665, 674, 761
CviJI RGCY 4 cut(s) 27, 84, 149, 524
CviKI_1 RGCY 4 cut(s) 27, 84, 149, 524
CviQI GTAC 2 cut(s) 68, 295
DdeI CTNAG 2 cut(s) 285, 401
DpnI GATC 4 cut(s) 51, 166, 491, 757
DpnII GATC 4 cut(s) 49, 164, 489, 755
EaeI YGGCCR 1 cut(s) 82
Eam1104I CTCTTC 3 cut(s) 500, 543, 612
EarI CTCTTC 3 cut(s) 500, 543, 612
Eco47I GGWCC 3 cut(s) 180, 389, 648
Eco57I CTGAAG 1 cut(s) 524
EcoO109I RGGNCCY 1 cut(s) 389
EcoRII CCWGG 2 cut(s) 183, 751
EcoT22I ATGCAT 1 cut(s) 581
Esp3I CGTCTC 1 cut(s) 332
FaeI CATG 6 cut(s) 92, 409, 655, 668, 677, 764
FatI CATG 6 cut(s) 88, 405, 651, 664, 673, 760
FauNDI CATATG 1 cut(s) 686
FbaI TGATCA 1 cut(s) 489
FokI GGATG 2 cut(s) 494, 653
FspBI CTAG 1 cut(s) 71
GlaI GCGC 1 cut(s) 162
GsuI CTGGAG 1 cut(s) 121
HaeIII GGCC 1 cut(s) 84
HgaI GACGC 1 cut(s) 508
HhaI GCGC 1 cut(s) 163
Hin1II CATG 6 cut(s) 92, 409, 655, 668, 677, 764
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HinfI GANTC 3 cut(s) 377, 439, 706
HphI GGTGA 1 cut(s) 701
Hpy166II GTNNAC 1 cut(s) 190
Hpy188I TCNGA 2 cut(s) 173, 199
Hpy188III TCNNGA 8 cut(s) 17, 89, 138, 400, 475, 493, 614, 674
Hpy8I GTNNAC 1 cut(s) 190
HpyAV CCTTC 2 cut(s) 742, 761
HpyCH4III ACNGT 2 cut(s) 326, 412
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 4 cut(s) 190, 579, 660, 684
HpyF10VI GCNNNNNNNGC 2 cut(s) 661, 726
HpyF3I CTNAG 2 cut(s) 285, 401
HpySE526I ACGT 1 cut(s) 45
Hsp92II CATG 6 cut(s) 92, 409, 655, 668, 677, 764
HspAI GCGC 1 cut(s) 161
Ksp22I TGATCA 1 cut(s) 489
Kzo9I GATC 4 cut(s) 49, 164, 489, 755
LweI GCATC 3 cut(s) 226, 472, 566
MaeI CTAG 1 cut(s) 71
MaeII ACGT 1 cut(s) 45
MaeIII GTNAC 3 cut(s) 215, 268, 707
MalI GATC 4 cut(s) 51, 166, 491, 757
MboI GATC 4 cut(s) 49, 164, 489, 755
MboII GAAGA 7 cut(s) 144, 186, 461, 508, 517, 560, 629
MhlI GDGCHC 1 cut(s) 192
MluCI AATT 2 cut(s) 231, 363
MlyI GAGTC 1 cut(s) 715
MmeI TCCRAC 1 cut(s) 610
Mph1103I ATGCAT 1 cut(s) 581
MseI TTAA 3 cut(s) 303, 540, 784
MslI CAYNNNNRTG 1 cut(s) 665
MspR9I CCNGG 2 cut(s) 185, 753
MvaI CCWGG 2 cut(s) 185, 753
MvnI CGCG 2 cut(s) 161, 163
MwoI GCNNNNNNNGC 2 cut(s) 661, 726
NdeI CATATG 1 cut(s) 686
NdeII GATC 4 cut(s) 49, 164, 489, 755
NlaIII CATG 6 cut(s) 92, 409, 655, 668, 677, 764
NlaIV GGNNCC 1 cut(s) 182
NmeAIII GCCGAG 1 cut(s) 60
NmuCI GTSAC 2 cut(s) 268, 707
NsiI ATGCAT 1 cut(s) 581
NspI RCATGY 1 cut(s) 668
NspV TTCGAA 3 cut(s) 129, 458, 699
OliI CACNNNNGTG 1 cut(s) 665
PagI TCATGA 2 cut(s) 88, 673
PcsI WCGNNNNNNNCGW 2 cut(s) 15, 621
PfeI GAWTC 2 cut(s) 377, 439
PleI GAGTC 1 cut(s) 714
PpsI GAGTC 1 cut(s) 714
PpuMI RGGWCCY 1 cut(s) 389
Psp5II RGGWCCY 1 cut(s) 389
Psp6I CCWGG 2 cut(s) 183, 751
PspGI CCWGG 2 cut(s) 183, 751
PspN4I GGNNCC 1 cut(s) 182
PspPI GGNCC 3 cut(s) 180, 389, 648
PspPPI RGGWCCY 1 cut(s) 389
RsaI GTAC 2 cut(s) 69, 296
RsaNI GTAC 2 cut(s) 68, 295
RseI CAYNNNNRTG 1 cut(s) 665
SaqAI TTAA 3 cut(s) 303, 540, 784
Sau3AI GATC 4 cut(s) 49, 164, 489, 755
Sau96I GGNCC 3 cut(s) 180, 389, 648
ScaI AGTACT 1 cut(s) 69
SchI GAGTC 1 cut(s) 715
ScrFI CCNGG 2 cut(s) 185, 753
SduI GDGCHC 1 cut(s) 192
SfaNI GCATC 3 cut(s) 226, 472, 566
SfcI CTRYAG 1 cut(s) 144
SfuI TTCGAA 3 cut(s) 129, 458, 699
SinI GGWCC 3 cut(s) 180, 389, 648
SmiMI CAYNNNNRTG 1 cut(s) 665
SmlI CTYRAG 1 cut(s) 473
SmoI CTYRAG 1 cut(s) 473
Sse9I AATT 2 cut(s) 231, 363
SsiI CCGC 1 cut(s) 159
SspI AATATT 1 cut(s) 98
SspMI CTAG 1 cut(s) 71
StyD4I CCNGG 2 cut(s) 183, 751
TaaI ACNGT 2 cut(s) 326, 412
TaiI ACGT 1 cut(s) 48
TaqI TCGA 6 cut(s) 129, 458, 559, 615, 699, 735
TasI AATT 2 cut(s) 231, 363
TatI WGTACW 2 cut(s) 67, 294
TfiI GAWTC 2 cut(s) 377, 439
Tru1I TTAA 3 cut(s) 303, 540, 784
Tru9I TTAA 3 cut(s) 303, 540, 784
TscAI CASTG 2 cut(s) 120, 331
TseFI GTSAC 2 cut(s) 268, 707
Tsp45I GTSAC 2 cut(s) 268, 707
TspDTI ATGAA 3 cut(s) 434, 662, 690
TspGWI ACGGA 2 cut(s) 193, 718
TspRI CASTG 2 cut(s) 120, 331
VneI GTGCAC 1 cut(s) 188
VpaK11BI GGWCC 3 cut(s) 180, 389, 648
XceI RCATGY 1 cut(s) 668
XspI CTAG 1 cut(s) 71
ZrmI AGTACT 1 cut(s) 69
Zsp2I ATGCAT 1 cut(s) 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.