MD10G1083000.v1.1

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007484
Physical Location & Seq
Forward (+)
12177334 .. 12178215
882 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1083000.v1.1.491

Sequence Viewer

Length: 882 bp
ATGCTAGCGCAGTTGCGTCTATTGATATTCACTATTGCTGCTAGCTTTTTTTATTTTTTATTTTTTAATCGTCGGGTGACTTGCGATATGGAAGACGTCTTTCACGAGTTGAGCCAAAGCTTTAATTACTGGCACGTTGATCCCCTTGACACACAAGTACTAGACCCCCTCGGCCCTCATGAGAAATATTTCCCTTGTGTTCCCCTGGAAATCTACTTTGAAGTCTCCAGAAACTACAGCTACTTCGACCGCGATCTCTCAGAAGACGGAACCCTCACCCTGGTGCACCAATCTGAAGTACAAGTGAGAAGTTACCAAAGCAAAATAGTTGATGCGTCCTGGTTACAAACAAGAAACGCATACGTCAGACCCTATCTTTCTCGGGTGCTGTCCTTTCTTAAAATCCACGAGGATGTGCACAGTGGTATGGTAGAGACGATTATAGAAGGGGGTATCCGAATTAGAAACTTGGATTCCAAGAAAGGTCCTAAAGTCCTGCGCCTGAGGGTTGGCATACAAATATACCATATCCAGATTCGTTGTGAGGACTGCTCGAAACAAAGAGAACTCGAAAGGGATGCGTTGATCAAGAAGATGCTAAAGAGGGTTAGAGTGGTGGCTGATGCCGATGATGTTAATGAGGAGGAGAGAGGTCGAGGAAGAAAAAGACAACCCGTTGTGCGTGAAAGTGAGGTTTGCCCTATTTGTCTGGAAAAGTTTGTTGTGGGATCAGAGGATGTAACATCCATGCCTTGCACACATATGTTTCATGAAAACTGCATATGGAGATGGTTCAAACAGAGTCACCTTAATTGCCCTATTTGCCGCTTCGAGATGTTTATTGAACTTGGATCACAAGGGGAAGGAGACATTAGGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

294

Amino Acids

34.51

Weight (kDa)

6.38

Isoelectric Point (pI)

50.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 233 - 276 3.2e-12 Ring finger domain
zf-RING_11 PF17123 233 - 261 1.2e-08 RING-like zinc finger
zf-RING_UBOX PF13445 233 - 273 1.7e-08 RING-type zinc-finger
zf-C3HC4_2 PF13923 233 - 275 7.7e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 233 - 275 1.9e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 99
AccII CGCG 1 cut(s) 252
AciI CCGC 2 cut(s) 250, 826
AclWI GGATC 3 cut(s) 134, 736, 859
AcuI CTGAAG 1 cut(s) 315
AcyI GRCGYC 1 cut(s) 96
AfaI GTAC 2 cut(s) 159, 300
AfiI CCNNNNNNNGG 1 cut(s) 280
AgsI TTSAA 3 cut(s) 221, 796, 845
AjnI CCWGG 3 cut(s) 204, 279, 338
AleI CACNNNNGTG 1 cut(s) 281
AluBI AGCT 3 cut(s) 45, 120, 240
AluI AGCT 3 cut(s) 45, 120, 240
Alw21I GWGCWC 2 cut(s) 288, 420
Alw26I GTCTC 3 cut(s) 229, 428, 861
Alw44I GTGCAC 2 cut(s) 284, 416
AlwI GGATC 3 cut(s) 134, 736, 859
Ama87I CYCGRG 1 cut(s) 381
AoxI GGCC 1 cut(s) 172
ApaLI GTGCAC 2 cut(s) 284, 416
ApeKI GCWGC 1 cut(s) 38
Asp700I GAANNNNTTC 1 cut(s) 188
AspLEI GCGC 2 cut(s) 10, 501
AspS9I GGNCC 2 cut(s) 173, 485
AsuHPI GGTGA 3 cut(s) 88, 268, 797
AsuNHI GCTAGC 2 cut(s) 4, 41
AvaI CYCGRG 1 cut(s) 381
AvaII GGWCC 1 cut(s) 485
AxyI CCTNAGG 1 cut(s) 503
BaeGI GKGCMC 2 cut(s) 288, 420
BauI CACGAG 2 cut(s) 104, 407
BbsI GAAGAC 2 cut(s) 99, 270
Bbv12I GWGCWC 2 cut(s) 288, 420
BbvI GCAGC 1 cut(s) 25
BccI CCATC 1 cut(s) 783
BcgI CGANNNNNNTGC 2 cut(s) 560, 594
BciT130I CCWGG 3 cut(s) 206, 281, 340
BciVI GTATCC 1 cut(s) 464
BclI TGATCA 1 cut(s) 585
BcoDI GTCTC 3 cut(s) 229, 428, 861
BfaI CTAG 3 cut(s) 5, 42, 161
BfmI CTRYAG 1 cut(s) 235
BfuI GTATCC 1 cut(s) 464
BisI GCNGC 2 cut(s) 39, 826
BlsI GCNGC 2 cut(s) 40, 827
BmcAI AGTACT 1 cut(s) 159
Bme1390I CCNGG 3 cut(s) 206, 281, 340
Bme18I GGWCC 1 cut(s) 485
BmeT110I CYCGRG 1 cut(s) 381
BmgT120I GGNCC 2 cut(s) 173, 485
BmiI GGNNCC 1 cut(s) 271
BmrFI CCNGG 3 cut(s) 206, 281, 340
BmsI GCATC 4 cut(s) 322, 568, 585, 613
BmtI GCTAGC 2 cut(s) 8, 45
BpiI GAAGAC 2 cut(s) 99, 270
BplI GAGNNNNNCTC 2 cut(s) 536, 568
BpmI CTGGAG 1 cut(s) 211
BsaHI GRCGYC 1 cut(s) 96
BsaJI CCNNGG 3 cut(s) 169, 204, 279
Bsc4I CCNNNNNNNGG 1 cut(s) 280
Bse1I ACTGG 1 cut(s) 134
Bse21I CCTNAGG 1 cut(s) 503
BseBI CCWGG 3 cut(s) 206, 281, 340
BseDI CCNNGG 3 cut(s) 169, 204, 279
BseGI GGATG 4 cut(s) 418, 583, 742, 743
BseLI CCNNNNNNNGG 1 cut(s) 280
BseMII CTCAG 2 cut(s) 273, 494
BseNI ACTGG 1 cut(s) 134
BseRI GAGGAG 2 cut(s) 656, 659
BseSI GKGCMC 2 cut(s) 288, 420
BseXI GCAGC 1 cut(s) 25
Bsh1236I CGCG 1 cut(s) 252
Bsh1285I CGRYCG 1 cut(s) 250
BshFI GGCC 1 cut(s) 174
BsiEI CGRYCG 1 cut(s) 250
BsiHKAI GWGCWC 2 cut(s) 288, 420
BsiHKCI CYCGRG 1 cut(s) 381
BslI CCNNNNNNNGG 1 cut(s) 280
BsmAI GTCTC 3 cut(s) 229, 428, 861
BsmBI CGTCTC 1 cut(s) 428
BsnI GGCC 1 cut(s) 174
BsoBI CYCGRG 1 cut(s) 381
Bsp1286I GDGCHC 2 cut(s) 288, 420
Bsp143I GATC 5 cut(s) 139, 253, 585, 728, 851
BspACI CCGC 2 cut(s) 250, 826
BspANI GGCC 1 cut(s) 174
BspCNI CTCAG 2 cut(s) 272, 495
BspFNI CGCG 1 cut(s) 252
BspHI TCATGA 2 cut(s) 178, 769
BspLI GGNNCC 1 cut(s) 271
BspOI GCTAGC 2 cut(s) 8, 45
BspPI GGATC 3 cut(s) 134, 736, 859
BsrI ACTGG 1 cut(s) 134
BssECI CCNNGG 3 cut(s) 169, 204, 279
BssMI GATC 5 cut(s) 139, 253, 585, 728, 851
BssNI GRCGYC 1 cut(s) 96
BssSI CACGAG 2 cut(s) 104, 407
Bst2BI CACGAG 2 cut(s) 104, 407
Bst2UI CCWGG 3 cut(s) 206, 281, 340
Bst4CI ACNGT 1 cut(s) 422
BstACI GRCGYC 1 cut(s) 96
BstC8I GCNNGC 2 cut(s) 6, 43
BstDEI CTNAG 2 cut(s) 259, 503
BstF5I GGATG 4 cut(s) 418, 583, 742, 743
BstFNI CGCG 1 cut(s) 252
BstHHI GCGC 2 cut(s) 10, 501
BstKTI GATC 5 cut(s) 142, 256, 588, 731, 854
BstMAI GTCTC 3 cut(s) 229, 428, 861
BstMBI GATC 5 cut(s) 139, 253, 585, 728, 851
BstMCI CGRYCG 1 cut(s) 250
BstMWI GCNNNNNNNGC 1 cut(s) 822
BstNI CCWGG 3 cut(s) 206, 281, 340
BstSCI CCNGG 3 cut(s) 204, 279, 338
BstSFI CTRYAG 1 cut(s) 235
BstSLI GKGCMC 2 cut(s) 288, 420
BstUI CGCG 1 cut(s) 252
BstV1I GCAGC 1 cut(s) 25
BstV2I GAAGAC 2 cut(s) 99, 270
Bsu36I CCTNAGG 1 cut(s) 503
BsuI GTATCC 1 cut(s) 464
BsuRI GGCC 1 cut(s) 174
BtsCI GGATG 4 cut(s) 418, 583, 742, 743
BtsIMutI CAGTG 1 cut(s) 427
Cac8I GCNNGC 2 cut(s) 6, 43
CciI TCATGA 2 cut(s) 178, 769
CfoI GCGC 2 cut(s) 10, 501
Cfr13I GGNCC 2 cut(s) 173, 485
CseI GACGC 2 cut(s) 5, 324
Csp6I GTAC 2 cut(s) 158, 299
CviAII CATG 3 cut(s) 179, 748, 770
CviJI RGCY 6 cut(s) 45, 114, 120, 174, 240, 620
CviKI_1 RGCY 6 cut(s) 45, 114, 120, 174, 240, 620
CviQI GTAC 2 cut(s) 158, 299
DdeI CTNAG 2 cut(s) 259, 503
DpnI GATC 5 cut(s) 141, 255, 587, 730, 853
DpnII GATC 5 cut(s) 139, 253, 585, 728, 851
Eco47I GGWCC 1 cut(s) 485
Eco57I CTGAAG 1 cut(s) 315
Eco81I CCTNAGG 1 cut(s) 503
Eco88I CYCGRG 1 cut(s) 381
EcoO109I RGGNCCY 1 cut(s) 485
EcoRII CCWGG 3 cut(s) 204, 279, 338
Esp3I CGTCTC 1 cut(s) 428
FaeI CATG 3 cut(s) 182, 751, 773
FatI CATG 3 cut(s) 178, 747, 769
FauNDI CATATG 2 cut(s) 762, 782
FbaI TGATCA 1 cut(s) 585
Fnu4HI GCNGC 2 cut(s) 39, 826
FokI GGATG 4 cut(s) 425, 590, 730, 749
Fsp4HI GCNGC 2 cut(s) 39, 826
FspBI CTAG 3 cut(s) 5, 42, 161
GlaI GCGC 2 cut(s) 9, 500
GluI GCNGC 2 cut(s) 39, 826
GsuI CTGGAG 1 cut(s) 211
HaeIII GGCC 1 cut(s) 174
HgaI GACGC 2 cut(s) 5, 324
HhaI GCGC 2 cut(s) 10, 501
Hin1I GRCGYC 1 cut(s) 96
Hin1II CATG 3 cut(s) 182, 751, 773
Hin6I GCGC 2 cut(s) 8, 499
HinP1I GCGC 2 cut(s) 8, 499
HindIII AAGCTT 1 cut(s) 118
HinfI GANTC 3 cut(s) 473, 535, 802
HphI GGTGA 3 cut(s) 88, 268, 797
Hpy166II GTNNAC 2 cut(s) 286, 418
Hpy188I TCNGA 5 cut(s) 262, 295, 368, 458, 733
Hpy188III TCNNGA 8 cut(s) 104, 179, 228, 532, 589, 710, 770, 832
Hpy8I GTNNAC 2 cut(s) 286, 418
Hpy99I CGWCG 1 cut(s) 75
HpyAV CCTTC 2 cut(s) 440, 857
HpyCH4III ACNGT 1 cut(s) 422
HpyCH4IV ACGT 3 cut(s) 96, 135, 363
HpyCH4V TGCA 4 cut(s) 286, 418, 756, 780
HpyF10VI GCNNNNNNNGC 1 cut(s) 822
HpyF3I CTNAG 2 cut(s) 259, 503
HpySE526I ACGT 3 cut(s) 96, 135, 363
Hsp92I GRCGYC 1 cut(s) 96
Hsp92II CATG 3 cut(s) 182, 751, 773
HspAI GCGC 2 cut(s) 8, 499
Ksp22I TGATCA 1 cut(s) 585
Kzo9I GATC 5 cut(s) 139, 253, 585, 728, 851
Lsp1109I GCAGC 1 cut(s) 25
LweI GCATC 4 cut(s) 322, 568, 585, 613
MaeI CTAG 3 cut(s) 5, 42, 161
MaeII ACGT 3 cut(s) 96, 135, 363
MaeIII GTNAC 5 cut(s) 76, 311, 342, 739, 803
MalI GATC 5 cut(s) 141, 255, 587, 730, 853
MboI GATC 5 cut(s) 139, 253, 585, 728, 851
MboII GAAGA 4 cut(s) 104, 275, 604, 672
MhlI GDGCHC 2 cut(s) 288, 420
MluCI AATT 3 cut(s) 124, 459, 811
MlyI GAGTC 1 cut(s) 811
MroXI GAANNNNTTC 1 cut(s) 188
MseI TTAA 6 cut(s) 66, 123, 399, 636, 810, 880
MslI CAYNNNNRTG 3 cut(s) 281, 411, 761
MspR9I CCNGG 3 cut(s) 206, 281, 340
MvaI CCWGG 3 cut(s) 206, 281, 340
MvnI CGCG 1 cut(s) 252
MwoI GCNNNNNNNGC 1 cut(s) 822
NdeI CATATG 2 cut(s) 762, 782
NdeII GATC 5 cut(s) 139, 253, 585, 728, 851
NheI GCTAGC 2 cut(s) 4, 41
NlaIII CATG 3 cut(s) 182, 751, 773
NlaIV GGNNCC 1 cut(s) 271
NmeAIII GCCGAG 1 cut(s) 150
NmuCI GTSAC 2 cut(s) 76, 803
OliI CACNNNNGTG 1 cut(s) 281
PagI TCATGA 2 cut(s) 178, 769
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PdmI GAANNNNTTC 1 cut(s) 188
PfeI GAWTC 2 cut(s) 473, 535
PkrI GCNGC 2 cut(s) 40, 827
PleI GAGTC 1 cut(s) 810
PpsI GAGTC 1 cut(s) 810
PpuMI RGGWCCY 1 cut(s) 485
Psp5II RGGWCCY 1 cut(s) 485
Psp6I CCWGG 3 cut(s) 204, 279, 338
PspGI CCWGG 3 cut(s) 204, 279, 338
PspN4I GGNNCC 1 cut(s) 271
PspPI GGNCC 2 cut(s) 173, 485
PspPPI RGGWCCY 1 cut(s) 485
RsaI GTAC 2 cut(s) 159, 300
RsaNI GTAC 2 cut(s) 158, 299
RseI CAYNNNNRTG 3 cut(s) 281, 411, 761
SaqAI TTAA 6 cut(s) 66, 123, 399, 636, 810, 880
SatI GCNGC 2 cut(s) 39, 826
Sau3AI GATC 5 cut(s) 139, 253, 585, 728, 851
Sau96I GGNCC 2 cut(s) 173, 485
ScaI AGTACT 1 cut(s) 159
SchI GAGTC 1 cut(s) 811
ScrFI CCNGG 3 cut(s) 206, 281, 340
SduI GDGCHC 2 cut(s) 288, 420
SfaNI GCATC 4 cut(s) 322, 568, 585, 613
SfcI CTRYAG 1 cut(s) 235
SinI GGWCC 1 cut(s) 485
SmiMI CAYNNNNRTG 3 cut(s) 281, 411, 761
Sse9I AATT 3 cut(s) 124, 459, 811
SsiI CCGC 2 cut(s) 250, 826
SspI AATATT 1 cut(s) 188
SspMI CTAG 3 cut(s) 5, 42, 161
StyD4I CCNGG 3 cut(s) 204, 279, 338
TaaI ACNGT 1 cut(s) 422
TaiI ACGT 3 cut(s) 99, 138, 366
TaqI TCGA 5 cut(s) 246, 554, 570, 655, 831
TasI AATT 3 cut(s) 124, 459, 811
TatI WGTACW 2 cut(s) 157, 298
TauI GCSGC 1 cut(s) 828
TfiI GAWTC 2 cut(s) 473, 535
Tru1I TTAA 6 cut(s) 66, 123, 399, 636, 810, 880
Tru9I TTAA 6 cut(s) 66, 123, 399, 636, 810, 880
TscAI CASTG 1 cut(s) 427
TseFI GTSAC 2 cut(s) 76, 803
TseI GCWGC 1 cut(s) 38
Tsp45I GTSAC 2 cut(s) 76, 803
TspDTI ATGAA 2 cut(s) 758, 786
TspGWI ACGGA 1 cut(s) 282
TspRI CASTG 1 cut(s) 427
VneI GTGCAC 2 cut(s) 284, 416
VpaK11BI GGWCC 1 cut(s) 485
XmnI GAANNNNTTC 1 cut(s) 188
XspI CTAG 3 cut(s) 5, 42, 161
ZraI GACGTC 1 cut(s) 97
ZrmI AGTACT 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.